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152. Effect of PNPLA3 polymorphism on diagnostic performance of various noninvasive markers for diagnosing and staging nonalcoholic fatty liver disease

Author

Liu, Wen‐Yue, primary, Zheng, Kenneth I., additional, Pan, Xiao‐Yan, additional, Ma, Hong‐Lei, additional, Zhu, Pei‐Wu, additional, Wu, Xi‐Xi, additional, Rios, Rafael S., additional, Targher, Giovanni, additional, Byrne, Christopher D, additional, Wang, Xiao‐Dong, additional, Chen, Yong‐Ping, additional, and Zheng, Ming‐Hua, additional

Published
2019
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153. Genomic epidemiology of vancomycin resistantEnterococcus faecium(VREfm) in Latin America: Revisiting the global VRE population structure

Author

Rios, Rafael, primary, Reyes, Jinnethe, additional, Carvajal, Lina P., additional, Rincon, Sandra, additional, Panesso, Diana, additional, Planet, Paul J., additional, Echeverri, Aura M., additional, Dinh, An, additional, Kolokotronis, Sergios-Orestis, additional, Narechania, Apurva, additional, Tran, Truc T., additional, Munita, Jose M., additional, Murray, Barbara E., additional, Arias, Cesar A., additional, and Diaz, Lorena, additional

Published
2019
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154. Heavy and Light Chain Monitoring in High Risk Smoldering Multiple Myeloma Patients Included in the GEM-CESAR Trial: Comparison with Conventional and Minimal Residual Disease IMWG Response Assessment

Author

Puig, Noemi, primary, Contreras, Teresa, additional, Paiva, Bruno, additional, Cedena, María Teresa, additional, Pérez, José J, additional, Aires, Irene, additional, Agullo, Cristina, additional, Martinez-Lopez, Joaquin, additional, Rodriguez Otero, Paula, additional, Gonzalez De La Calle, Veronica, additional, Gonzalez, Marta Sonia, additional, Oriol, Albert, additional, Gutierrez, Norma C., additional, Rios, Rafael, additional, Rosiñol, Laura, additional, Alvarez, Miguel Angel, additional, Calasanz, Maria Jose, additional, Bargay, Joan, additional, Gonzalez, Ana Pilar, additional, Alegre, Adrián, additional, Escalante, Fernando, additional, Martínez, Rafael, additional, de la Rubia, Javier, additional, Teruel, Ana Isabel, additional, De Arriba, Felipe, additional, Palomera, Luis, additional, Hernández, Miguel-Teodoro, additional, López, Javier, additional, Martín, Jesús, additional, García Mateo, Aránzazu, additional, Ocio, Enrique M., additional, García-Sanz, Ramón, additional, Bladé, Joan, additional, Lahuerta, Juan Jose, additional, San-Miguel, Jesus F., additional, and Mateos, Maria-Victoria, additional

Published
2019
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155. Circulating Tumor Cells (CTCs) for Comprehensive and Multiregional Non-Invasive Genetic Characterization of Multiple Myeloma (MM)

Author

Garcés, Juan José, primary, Bretones, Gabriel, additional, Burgos, Leire, additional, Valdes-Mas, Rafael, additional, Alignani, Diego, additional, Álvarez Puente, Diana, additional, Goicoechea, Ibai, additional, García Álvarez, Miguel, additional, Garate, Sonia, additional, Rodriguez, Idoia, additional, Calasanz, Maria Jose, additional, Rodriguez, Paula, additional, Rios, Rafael, additional, Martinez-Lopez, Joaquin, additional, Millacoy, Pamela, additional, Palomera, Luis, additional, Del Orbe, Rafael, additional, Pérez, Albert, additional, Agirre, Xabier, additional, Flores-Montero, Juan, additional, Sanoja-Flores, Luzalba, additional, Orfao, Alberto, additional, El Omri, Halima, additional, Prosper, Felipe, additional, Lopez-Otin, Carlos, additional, San-Miguel, Jesús, additional, and Paiva, Bruno, additional

Published
2019
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156. Single-Cell Characterization of the Multiple Myeloma (MM) Immune Microenvironment Identifies CD27-Negative T Cells As Potential Source of Tumor-Reactive Lymphocytes

Author

Botta, Cirino, primary, Pérez Ruiz, Cristina, primary, Goicoechea, Ibai, primary, Puig, Noemi, primary, Cedena, María Teresa, primary, Cordon, Lourdes, primary, Zabaleta, Aintzane, primary, Burgos, Leire, primary, Maia, Catarina, primary, Rodríguez, Sara, primary, Rodriguez, Idoia, primary, Sarvide, Sarai, primary, Alignani, Diego, primary, Vilas-Zornoza, Amaia, primary, Lorenzo-Vivas, Erika, primary, Rosinol Dachs, Laura, primary, Oriol, Albert, primary, Blanchard, María Jesús, primary, Rios, Rafael, primary, Sureda, Anna, primary, Martínez, Rafael, primary, Martín, Jesús, primary, Bargay, Joan, primary, De La Rubia, Javier, primary, Rossi, Marco, primary, Tagliaferri, Pierosandro, primary, Tassone, Pierfrancesco, primary, Gentile, Massimo, primary, Merino, Juana, primary, Prosper, Felipe, primary, Orfao, Alberto, primary, Mateos, Maria-Victoria, primary, Lahuerta, Juan-José, primary, Bladé, Joan, primary, San-Miguel, Jesús, primary, and Paiva, Bruno, primary

Published
2019
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157. Qip-Mass Spectrometry in High Risk Smoldering Multiple Myeloma Patients Included in the GEM-CESAR Trial: Comparison with Conventional and Minimal Residual Disease IMWG Response Assessment

Author

Puig, Noemi, primary, Mateos, Maria-Victoria, primary, Contreras, Teresa, primary, Paiva, Bruno, primary, Cedena, María Teresa, primary, Pérez, José J, primary, Aires, Irene, primary, Agullo, Cristina, primary, Martinez-Lopez, Joaquin, primary, Rodriguez Otero, Paula, primary, Gonzalez De La Calle, Veronica, primary, Gonzalez, Marta Sonia, primary, Oriol, Albert, primary, Gutierrez, Norma C., primary, Rios, Rafael, primary, Rosiñol, Laura, primary, Alvarez, Miguel Angel, primary, Calasanz, Maria Jose, primary, Bargay, Joan, primary, Gonzalez, Ana Pilar, primary, Alegre, Adrián, primary, Escalante, Fernando, primary, Martínez, Rafael, primary, de la Rubia, Javier, primary, Teruel, Ana Isabel, primary, De Arriba, Felipe, primary, Palomera, Luis, primary, Hernández, Miguel-Teodoro, primary, López, Javier, primary, Martín, Jesús, primary, García Mateo, Aránzazu, primary, García-Sanz, Ramón, primary, Ocio, Enrique M., primary, Bladé, Joan, primary, Lahuerta, Juan-José, primary, and San-Miguel, Jesus F., primary

Published
2019
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158. Home chemical and microbial transitions across urbanization

Author

McCall, Laura-Isobel, primary, Callewaert, Chris, additional, Zhu, Qiyun, additional, Song, Se Jin, additional, Bouslimani, Amina, additional, Minich, Jeremiah J., additional, Ernst, Madeleine, additional, Ruiz-Calderon, Jean F., additional, Cavallin, Humberto, additional, Pereira, Henrique S., additional, Novoselac, Atila, additional, Hernandez, Jean, additional, Rios, Rafael, additional, Branch, OraLee H., additional, Blaser, Martin J., additional, Paulino, Luciana C., additional, Dorrestein, Pieter C., additional, Knight, Rob, additional, and Dominguez-Bello, Maria G., additional

Published
2019
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159. Evaluation of aquafitness exercise on the physical and mental health of older women: a pilot study.

Author

Perkins, Krystal M., Munguia, Nora, Angulo, Aracely, Anaya, Carlos, Rios, Rafael, and Velazquez, Luis

Subjects
PILOT projects, EXPERIMENTAL design, SEDENTARY lifestyles, AQUATIC exercises, CONFIDENCE intervals, FUNCTIONAL status, ANTHROPOMETRY, MENTAL health, DESCRIPTIVE statistics, BODY mass index, ODDS ratio, SWIMMING, ADIPOSE tissues
Abstract

Physical activity is a priority to improve health. However, a sedentary lifestyle is increasingly becoming the norm. For example, in Mexico, sedentarism has increased, especially among older women. This study evaluated the effects of aquafitness on the health of older women in Mexico. Healthy older women performed aquafitness exercise and were compared to a control group of comparable women. Outcome assessments performed at baseline and after 17-weeks included psychological and physical/anthropometric measures. Participants in aquafitness became more optimistic, lost more weight, body fat, and a subsequent decrease in BMI, compared to controls. The results suggest important avenues for future research. [ABSTRACT FROM AUTHOR]

Published
2021
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162. Proposta de classificação climática diária e de perfis higrotérmicos diários para medições térmicas em inspeções de fachada

Author

Rios, Rafael Barbosa and Zanoni, Vanda Alice Garcia

Subjects
Classificação climática, Inspeção predial, Comportamento higrotérmico, Fachadas de edificações, Revestimento cerâmico
Abstract

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Arquitetura e Urbanismo, Programa de Pós-Graduação em Arquitetura e Urbanismo, 2019. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Nas inspeções prediais em sistemas de fachadas, a alta exposição aos agentes climáticos e às variações higrotérmicas dos sistemas construtivos ao longo do dia podem interferir nos procedimentos de coleta de dados e na representatividade dos resultados. A ausência de diretrizes, critérios e ferramentas para a caracterização climática em medições in loco dificulta a realização dos procedimentos de inspeção, a comparação entre resultados e a análise da ação dos agentes climáticos sobre o comportamento higrotérmico das edificações e de seus sistemas. O objetivo deste trabalho é propor um método de classificação climática diária e perfis higrotérmicos diários de referência para medições térmicas em inspeções de fachadas em revestimento cerâmico em Brasília-DF. Com base nos arquivos climáticos TMY-EPW, foi definida uma classificação climática diária. Em seguida, a partir da simulação realizada no programa WUFI Pro, de 6 tipologias de fachadas com revestimento cerâmico, para um ano típico de Brasília-DF, foram emitidos dados horários que, por meio da identificação de padrões por técnicas estatísticas, permitiu compor perfis higrotérmicos diários característicos de um ano típico, o qual considera as variações entre os períodos do ano, as orientações da fachada e a intensidade dos agentes climáticos. A classificação climática diária proposta contribui para a caracterização destes agentes em qualquer dia do ano, de modo a permitir a verificação da representatividade dos dados coletados em medições térmicas, quando comparados com os dias típicos ou de referência, ou entre medições realizadas em diferentes momentos ou locais. Os resultados desta pesquisa mostraram que, por meio de uma ferramenta visual, a definição dos perfis higrotérmicos diários facilitou a compreensão da ação dos agentes de exposição sobre os sistemas de fachada, e contribui para o levantamento dos fatores relevantes para a realização de medições térmicas em fachadas de modo a resultar em procedimentos de coleta de dados mais confiáveis, menos extensos, mais práticos e menos onerosos. A partir destas ferramentas, foi possível propor diretrizes para medições térmicas de fachadas em Brasília-DF. In building’s façade survey, the high exposure to the climate’s agents and the façade’s daily hygrothermal behavior may interfere with in situ tests and their result’s reliability. The lack of guidelines, criteria and tools for climate’s characterization during in situ tests may affect the inspection’s procedures, the comparison between results and the climate effect on building hygrothermal behavior analysis. The main purpose is the establishment of a method for daily weather classification and daily hygrothermal profiles as tools for thermal measurements in ceramic tile façade’s inspection in Brasília-DF. Based on the typical meteorological year weather files - TMY, it was developed the Daily Weather Classification. After that, the façade’s hygrothermal behavior for a typical year, for 6 different façade’s systems, was acquired by the software WUFI Pro. The data was applied on a statistical analysis, which identify patterns and allowed to establish daily hygrothermal profiles. Those profiles consider the year period, the façade’s orientation and the climate’s agents’ intensity. The Daily Weather Classification allows the characterization of climate’s agents, in any day of the year, which helps to verify the data relevance by comparing with typical project days, reference data or, also, other in situ measured data. The research results indicate that, by the establishment of visual tool, the daily hygrothermal profiles, there was a contribution for the comprehension of the influence of climate’s agents into building’s facades, which may lead to more practical, cheaper and faster façade thermal measurement’s procedures. Also, based on those tools, it was defined guidelines for thermal measurements in building’s facades in Brasília-DF.

Published
2019

163. Caso: Coroimport

Author

Urrunaga Rios, Rafael Alonso and Gonzales Huerta, Raúl Edgardo

Subjects
purl.org/pe-repo/ocde/ford#5.02.03 [http], Ferretería -- Comercialización -- Planificación estratégica -- Estudio de casos, Empresas industriales -- Planificación estratégica -- Estudio de casos, Coroimport -- Planificación estratégica -- Estudio de casos
Abstract

El presente trabajo de investigación es un caso que tiene como finalidad destacar las rutas de crecimiento de Coroimport, empresa peruana dedicada a la comercialización y distribución de productos ferreteros e industriales, y señalar los aspectos relevantes de análisis para la toma de una decisión conveniente.

Published
2019

164. LiaR-Independent Pathways to Daptomycin Resistance in Enterococcus faecalis Reveal A Multilayer Defense Against Cell Envelope Antibiotics

Author

Miller, William R., Tran, Truc T., Diaz, Lorena, Rios, Rafael, Khan, Ayesha, Reyes, Jinnethe, Prater, Amy G., Panesso, Diana, Shamoo, Yousif, and Arias, Cesar A.

Subjects
Whole Genome Sequencing, Ceftriaxone, Adaptation, Biological, Gene Expression Regulation, Bacterial, Microbial Sensitivity Tests, Article, Anti-Bacterial Agents, Bacitracin, Bacterial Proteins, Daptomycin, Drug Resistance, Bacterial, Mutation, Enterococcus faecalis, Serial Passage
Abstract

The lipopeptide antibiotic daptomycin (DAP) is a key drug against serious enterococcal infections, but the emergence of resistance in the clinical setting is a major concern. The LiaFSR system plays a prominent role in the development of DAP resistance (DAP-R) in enterococci, and blocking this stress response system has been proposed as a novel therapeutic strategy. In this work, we identify LiaR-independent pathways in Enterococcus faecalis that regulate cell membrane adaptation in response to antibiotics. We adapted E. faecalis OG1RF (a laboratory strain) and S613TM (a clinical strain) lacking liaR to increasing concentrations of DAP, leading to the development of DAP-R and elevated MICs to bacitracin and ceftriaxone. Whole genome sequencing identified changes in the YxdJK two-component regulatory system and a putative fatty acid kinase (dak) in both DAP-R strains. Deletion of the gene encoding the YxdJ response regulator in both the DAP-R mutant and wild-type OG1RF decreased MICs to DAP, even when a functional LiaFSR system was present. Mutations in dak were associated with slower growth, decreased membrane fluidity and alterations of cell morphology. These findings suggest that overlapping stress response pathways can provide protection against antimicrobial peptides in E. faecalis at a significant cost in bacterial fitness.

Published
2019

165. A continuous compact MOSFET model for fully- and partially-depleted SOI devices

Author

Sleight, Jeffrey W. and Rios, Rafael

Subjects
Metal oxide semiconductor field effect transistors -- Models, Electric circuit analysis -- Models, Business, Electronics, Electronics and electrical industries
Abstract

A continuous compact metal oxide semiconductor field effect transistor (MOSFET) model that considers the body depletion status of MOSFETs created using space-object identification technology is developed. It is based on the exact first integration of the one-dimensional Poisson equation. This model immediately accounts for changes in partially-depleted and fully-depleted behavior during the operation of the device.

Published
1998

166. P-090: Immune biomarkers of survival and infectionrelated mortality in multiple myeloma (MM) patients treated with lenalidomide and dexamethasone (Rd)

Author

Maia, Catarina, Puig, Noemí, Cedena, Maria-Teresa, Gutiérrez, Norma, Calassanz, María-J, Martín-Ramos, Maria-L, Garcia, Miguel Teodoro Hernandez, Rosiñol, Laura, González, Mª Esther, de la Fuente, Felipe de Arriba, Oriol, Albert, González, Verónica, Escalante, Fernando, De la Rubia, Javier, Mesa, Mercedes Gironella, Ríos, Rafael, García-Sánchez, Ricarda, Arguiñano, José-M, Alegre, Adrián, San-Miguel, Jesus, Palacios, Juan José Lahuerta, Blade, Joan, Niesvizky, Ruben, Mateos, María-Victoria, and Paiva, Bruno

Published
2022
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167. OAB-040: BUMEL vs MEL-200 prior autologous transplant for patients with newly diagnosed multiple myeloma previously treated with bortezomib, lenalidomide and dexamethasone: final results of a phase 3 trial

Author

Palacios, Juan José Lahuerta, Ubieto, Ana Jiménez, Rosiñol, Laura, Paiva, Bruno, López, Joaquín Martinez, Cedena, María Teresa, Puig, Noemí, Ríos, Rafael, Oriol, Albert, Blanchard, María Jesús, Bargay, Joan, Martín, Jesús, martinez, Rafael, Sureda, Anna, De la Rubia, Javier, Hernández, Miguel teodoro, Cabañas, Valentín, krsnik, Isabel, Palomera, Luis, Casado, Felipe, Gonzalez-Montes, Yolanda, de la Fuente, Felipe de Arriba, Mateos, María-Victoria, San-Miguel, Jesus, and Blade, Joan

Published
2022
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168. An accurate gate length extraction method for sub-quarter micron MOSFET's

Author

Cheng-Liang Huang, Faricelli, John V., Antoniadis, Dimitri A., Khalil, Nadim A., and Rios, Rafael A.

Subjects
Metal oxide semiconductor field effect transistors -- Design and construction, Simulation methods -- Usage, Capacitors -- Research, Business, Electronics, Electronics and electrical industries
Abstract

A novel method obtains accurate gate lengths for sub-quarter micron MOSFET applications by comparing measured and simulated gate-to-source/drain capacitances. Inclusion of the 2-D field effect on fringing capacitance, the polysilicon depletion and the quantum-well effects in the capacitance allow an accurate determination of the polysilicon gate length. The effects of the polysilicon depletion and quantum-well on the thin gate oxide capacitance are studied. The inaccuracy involved in determining the fringing capacitances is emphasized.

Published
1996

169. FNDC5 polymorphism influences the association between sarcopenia and liver fibrosis in adults with biopsy-proven non-alcoholic fatty liver disease.

Author

Gao, Feng, Zheng, Kenneth I., Zhu, Pei-Wu, Li, Yang-Yang, Ma, Hong-Lei, Li, Gang, Tang, Liang-Jie, Rios, Rafael S., Liu, Wen-Yue, Pan, Xiao-Yan, Targher, Giovanni, Byrne, Christopher D., Chen, Yong-Ping, and Zheng, Ming-Hua

Subjects
CONFIDENCE intervals, FATTY liver, GENETIC polymorphisms, SARCOPENIA, FIBROSIS, LIVER diseases, GENOTYPES, MEMBRANE proteins, LOGISTIC regression analysis, ODDS ratio
Abstract

The FNDC5 gene encodes the fibronectin type III domain-containing protein 5 that is a membrane protein mainly expressed in skeletal muscle, and the FNDC5 rs3480 polymorphism may be associated with liver disease severity in non-alcoholic fatty liver disease (NAFLD). We investigated the influence of the FNDC5 rs3480 polymorphism on the relationship between sarcopenia and the histological severity of NAFLD. A total of 370 adult individuals with biopsy-proven NAFLD were studied. The association between the key exposure sarcopenia and the outcome liver histological severity was investigated by binary logistic regression. Stratified analyses were undertaken to examine the impact of FNDC5 rs3480 polymorphism on the association between sarcopenia and the severity of NAFLD histology. Patients with sarcopenia had more severe histological grades of steatosis and a higher prevalence of significant fibrosis and definite non-alcoholic steatohepatitis than those without sarcopenia. There was a significant association between sarcopenia and significant fibrosis (adjusted OR 2·79, 95 % CI 1·31, 5·95, P = 0·008), independent of established risk factors and potential confounders. Among patients with sarcopenia, significant fibrosis occurred more frequently in the rs3480 AA genotype carriers than in those carrying the FNDC5 rs3480 G genotype (43·8 v. 17·2 %, P = 0·031). In the association between sarcopenia and liver fibrosis, there was a significant interaction between the FNDC5 genotype and sarcopenia status (P value for interaction = 0·006). Sarcopenia is independently associated with significant liver fibrosis, and the FNDC5 rs3480 G variant influences the association between sarcopenia and liver fibrosis in patients with biopsy-proven NAFLD. [ABSTRACT FROM AUTHOR]

Published
2021
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171. Modeling the polysilicon depletion effect and its impact on submicrometer CMOS circuit performance

Author

Arora, Narain D., Rios, Rafael, and Huang, Cheng-Liang

Subjects
Metal oxide semiconductor field effect transistors -- Models, Polysilicon -- Analysis, Business, Electronics, Electronics and electrical industries
Abstract

An effective analytical metal oxide semiconductor field effect transistor model for submicrometer devices accounts for the polysilicon depletion effect and precisely predicts both 2-D simulated and measured current-voltage and capacitance-voltage characteristics for different polysilicon gate concentrations. The model has been incorporated in the circuit simulator SPICE to analyze circuit performance due to polydepletion using inverter type circuits with varying loading conditions.

Published
1995

172. PCIM: a physically based continuous short-channel IGFET model for circuit simulation

Author

Arora, Narain D., Rios, Rafael, Huang, Cheng-Liang, and Raol, Kartik

Subjects
Field-effect transistors -- Models, Integrated circuits -- Analysis, Business, Electronics, Electronics and electrical industries
Abstract

A physically based, analytical and continuous IGFET model with velocity field relations for carriers is developed for short-channel devices with sub-half micron channel lengths. Channel length modulation expressions coupled to velocity-field equations generate precise drain conductance values and intrinsic gate capacitances. The temperature dependence of the device lies between 0 and 120 degrees C. The device has improved convergence characteristics.

Published
1994

173. Requirements for accurate MOS-SOI device simulations

Author

Rios, Rafael, Amantea, Robert, Smeltzer, Ronald K., and Rothwarf, Allen

Subjects
Metal oxide semiconductors -- Research, Silicon-on-isolator -- Research, Numerical analysis -- Methods, Boundary value problems -- Research, Business, Electronics, Electronics and electrical industries
Abstract

Three numerical analysis methods are presented for overcoming the device simulation problem posed by the floating substrate region in metal oxide semiconductor (MOS) silicon-on-insulator transistors. Models of carrier mobilities and lifetime degradation are also introduced. The techniques are verified via a comparison between theoretical and measured current-voltage characteristics of n-MOS silicon-on-sapphire devices.

Published
1992

175. Clinical Impact of Next Generation Flow in Bone Marrow VsQip-Mass Spectrometry in Peripheral Blood to Assess Minimal Residual Disease in Newly Diagnosed Multiple Myeloma Patients Receiving Maintenance As Part of the GEM2014MAIN Trial

Author

Puig, Noemi, Agulló, Cristina, Contreras Sanfeliciano, Teresa, Paiva, Bruno, Cedena, María Teresa, Rosinol Dachs, Laura, García-Sanz, Ramón, Martínez-López, Joaquín, Oriol, Albert, Blanchard, María Jesús, Rios, Rafael, Martin, Jesus, Sureda, Anna, Hernández, Miguel, de la Rubia, Javier, Moraleda, Jose Maria, De Arriba, Felipe, Palomera, Luis, Iñigo, Belén, Bargay, Joan, Bladé Creixenti, Joan, San-Miguel, Jesús, Lahuerta, Juan Jose, and Mateos, María-Victoria

Published
2022
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176. In Vivo Resistance to Ceftolozane/Tazobactam in Pseudomonas aeruginosa Arising by AmpC- and Non-AmpC-Mediated Pathways

Author

Skoglund, Erik, Abodakpi, Henrietta, Rios, Rafael, Diaz, Lorena, De La Cadena, Elsa, Dinh, An Q., Ardila, Javier, Miller, William R., Munita, Jose M., Arias, Cesar A., Tam, Vincent H., and Tran, Truc T.

Subjects
Article Subject, polycyclic compounds, bacteria, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses
Abstract

Two pairs of ceftolozane/tazobactam susceptible/resistant P. aeruginosa were isolated from 2 patients after exposure to β-lactams. The genetic basis of ceftolozane/tazobactam resistance was evaluated, and β-lactam-resistant mechanisms were assessed by phenotypic assays. Whole genome sequencing identified mutations in AmpC including the mutation (V213A) and a deletion of 7 amino acids (P210–G216) in the Ω-loop. Phenotypic assays showed that ceftolozane/tazobactam resistance in the strain with AmpCV213A variant was associated with increased β-lactamase hydrolysis activity. On the other hand, the deletion of 7 amino acids in the Ω-loop of AmpC did not display enhanced β-lactamase activity. Resistance to ceftolozane/tazobactam in P. aeruginosa is associated with changes in AmpC; however, the apparent loss of β-lactamase activity in AmpC∆7 suggests that non-AmpC mechanisms could play an important role in resistance to β-lactam/β-lactamase inhibitor combinations.

Published
2018
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177. 612. Molecular Mechanisms Leading to Ceftolozane–Tazobactam Resistance in Clinical Isolates of Pseudomonas aeruginosa from Five Latin American Countries

Author

Mojica, Maria F, Rios, Rafael, De La Cadena, Elsa, Correa, Adriana, Diaz, Lorena, Millan, Lina V, Hernandez, Angie K, Reyes, Jinnethe, Hernández-Gómez, Cristhian, Radice, Marcela A, Castañeda-Méndez, Paulo, Jaime-Villalón, Diego A, Gales, Ana C, Munita, Jose M, López, Catalina, Rojas Rojas, Monica Maria, and Virginia Villegas, Maria

Subjects
Abstracts, Poster Abstracts
Abstract

Background Ceftolozane–tazobactam (C/T) is a combination of an antipseudomonal cephalosporin with a known β-lactamase inhibitor, with a potent in vitro activity against P. aeruginosa (Pae), without activity against carbapenemases. Among the mechanisms of resistance to C/T that have emerged, substitutions in the Pseudomonal-derived cephalosporinase (PDC), in AmpR, and in some ESBLs, are the most commonly described. The aim of this study was to identify the molecular mechanisms responsible for the in vitro non-susceptibility (NS) to C/T in a group of clinical Pae strains from Latin America. Methods Clinical Pae isolates (n = 508) were collected between January 2016 and October 2017 from 20 hospitals located in Argentina, Brazil, Chile, Colombia, and Mexico. Minimum inhibitory concentrations (MICs) to C/T were determined by standard broth microdilution and interpreted according to CLSI M100 S28 breakpoints. Production of carbapenemases in Pae isolates displaying NS to C/T was assessed by carbaNP® followed by PCR to detect blaKPC, blaNDM-1, blaVIM and blaIMP. Illumina whole-genome sequencing (WGS) was performed for isolates in which NS to C/T was not mediated by carbapenemases. The presence of mutations in PDC, AmpR, oprD and dacB as compared with PAO1, was evaluated. Results According to the CLSI breakpoints, 162/508 (32%) Pae isolates demonstrated NS to C/T. Due to the absence of growth, only 151/162 were further processed. Table 1 summarizes the results obtained by carbaNP®, PCR and WGS performed on these isolates. In 53% of the isolates, NS to C/T was explained by the production of at least one carbapenemase, KPC or VIM. WGS revealed that in addition to substitutions in PDC and AmpR, some isolates carried mutations in oprD and dacB (encoding PBP4) genes. The molecular mechanism of resistance in 4/56 isolates is yet to be determined. Conclusion Carbapenemase production is the most common mechanism of resistance to C/T detected in this study. VIM and KPC were detected in equal proportions, while none of the isolates was found to carry IMP or NDM. Further studies are warranted to establish the role of the novel substitutions found in PDC and AmpR, as well as the degree to which the mutations found in oprD and dacB contribute to the NS phenotype in some isolates. Disclosures All authors: No reported disclosures.

Published
2019

179. Transcriptional profiling of circulating tumor cells in multiple myeloma: a new model to understand disease dissemination

Author

Garcés, Juan-Jose, primary, Simicek, Michal, additional, Vicari, Marco, additional, Brozova, Lucie, additional, Burgos, Leire, additional, Bezdekova, Renata, additional, Alignani, Diego, additional, Calasanz, Maria-Jose, additional, Growkova, Katerina, additional, Goicoechea, Ibai, additional, Agirre, Xabier, additional, Pour, Ludek, additional, Prosper, Felipe, additional, Rios, Rafael, additional, Martinez-Lopez, Joaquin, additional, Millacoy, Pamela, additional, Palomera, Luis, additional, Del Orbe, Rafael, additional, Perez-Montaña, Albert, additional, Garate, Sonia, additional, Blanco, Laura, additional, Lasa, Marta, additional, Maiso, Patricia, additional, Flores-Montero, Juan, additional, Sanoja-Flores, Luzalba, additional, Chyra, Zuzana, additional, Vdovin, Alexander, additional, Sevcikova, Tereza, additional, Jelinek, Tomas, additional, Botta, Cirino, additional, El Omri, Halima, additional, Keats, Jonathan, additional, Orfao, Alberto, additional, Hajek, Roman, additional, San-Miguel, Jesus F., additional, and Paiva, Bruno, additional

Published
2019
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182. 1827. Genetic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates Associated with the Development of Reduced Susceptibility to Vancomycin from Latin America

Author

Liliana Vargas Ardila, Monica, primary, Castro-Cardozo, Betsy E, additional, Rios, Rafael, additional, Hernandez, Angie K, additional, Millan, Lina V, additional, Carvajal, Lina P, additional, Seas, Carlos, additional, Munita, Jose M, additional, Arias, Cesar A, additional, Reyes, Jinnethe, additional, and Diaz, Lorena, additional

Published
2019
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183. 556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America

Author

Martínez, Jose R W, primary, Diaz, Lorena, additional, Rojas, Marcelo, additional, Rios, Rafael, additional, Hanson, Blake, additional, Rivas, Lina M, additional, Spencer, Maria, additional, Moustafa, Ahmed M, additional, Araos Bralic, Rafael, additional, Peters, Anne, additional, Reyes, Jinnethe, additional, Carvajal, Lina P, additional, Luna, Carlos, additional, Salles, Mauro, additional, Alvarez, Carlos, additional, Labarca, Jaime, additional, Seas, Carlos, additional, Guzmán, Manuel, additional, Planet, Paul J, additional, Arias, Cesar A, additional, and Munita, Jose, additional

Published
2019
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184. 1546. Efficacy of Daptomycin Combinations Against Daptomycin-Resistant Enterococcus faecium Differs by β-lactam

Author

Kebriaei, Razieh, primary, Stamper, Kyle, additional, Rice, Seth, additional, Maassen, Philip, additional, Singh, Kavindra V, additional, Dinh, An Q, additional, Rios, Rafael, additional, Diaz, Lorena, additional, Murray, Barbara E, additional, Khan, Ayesha, additional, Tran, Truc T, additional, Arias, Cesar A, additional, and Rybak, Michael J, additional

Published
2019
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185. 625. Genomic Epidemiology of Carbapenem-Resistant Enterobacteriaceae from Colombia: A Prospective Multicenter Study

Author

Reyes, Jinnethe, primary, Diaz, Lorena, additional, Carvajal, Lina P, additional, Rios, Rafael, additional, Millan, Lina V, additional, Echeverri, Aura M, additional, Hernandez, Angie K, additional, Vargas, Sandra, additional, Salcedo, Soraya, additional, Marin, Adriana, additional, Mora, Laura, additional, Ordonez Diaz, Karen M, additional, Cristancho Quintero, Edilberto, additional, Valderrama, Sandra, additional, Elena Ariza, Beatriz, additional, Cortes, Gloria, additional, Rojas, Laura J, additional, Chambers, Henry F, additional, Fowler, Vance G, additional, Kreiswirth, Barry, additional, Virginia Villegas, Maria, additional, Bonomo, Robert A, additional, Hanson, Blake, additional, van Duin, David, additional, and Arias, Cesar A, additional

Published
2019
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186. Corrigendum to “In Vivo Resistance to Ceftolozane/Tazobactam in Pseudomonas aeruginosa Arising by AmpC- and Non-AmpC-Mediated Pathways”

Author

Skoglund, Erik, primary, Abodakpi, Henrietta, additional, Rios, Rafael, additional, Diaz, Lorena, additional, De La Cadena, Elsa, additional, Dinh, An Q., additional, Ardila, Javier, additional, Miller, William R., additional, Munita, Jose M., additional, Arias, Cesar A., additional, Tam, Vincent H., additional, and Tran, Truc T., additional

Published
2019
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188. Extensively Drug-Resistant Pseudomonas aeruginosa ST309 Harboring Tandem Guiana Extended Spectrum β-Lactamase Enzymes: A Newly Emerging Threat in the United States

Author

Khan, Ayesha, primary, Tran, Truc T, additional, Rios, Rafael, additional, Hanson, Blake, additional, Shropshire, William C, additional, Sun, Zhizeng, additional, Diaz, Lorena, additional, Dinh, An Q, additional, Wanger, Audrey, additional, Ostrosky-Zeichner, Luis, additional, Palzkill, Timothy, additional, Arias, Cesar A, additional, and Miller, William R, additional

Published
2019
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189. Disrupting Membrane Adaptation Restores In Vivo Efficacy of Antibiotics Against Multidrug-Resistant Enterococci and Potentiates Killing by Human Neutrophils

Author

Rincon, Sandra, primary, Panesso, Diana, additional, Miller, William R, additional, Singh, Kavindra V, additional, Cruz, Melissa R, additional, Khan, Ayesha, additional, Dinh, An Q, additional, Diaz, Lorena, additional, Rios, Rafael, additional, Shamoo, Yousif, additional, Reyes, Jinnethe, additional, Tran, Truc T, additional, Garsin, Danielle A, additional, and Arias, Cesar A, additional

Published
2019
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191. Mutations in cdsA and pgsA Correlate with Daptomycin Resistance in Streptococcus mitis and S. oralis

Author

Tran, Truc T., primary, Mishra, Nagendra N., additional, Seepersaud, Ravin, additional, Diaz, Lorena, additional, Rios, Rafael, additional, Dinh, An Q., additional, Garcia-de-la-Maria, Cristina, additional, Rybak, Michael J., additional, Miro, Jose M., additional, Shelburne, Samuel A., additional, Sullam, Paul M., additional, Bayer, Arnold S., additional, and Arias, Cesar A., additional

Published
2019
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192. Extremely Drug-ResistantPseudomonas aeruginosaST309 Harboring Tandem GES Enzymes: A Newly Emerging Threat in the United States

Author

Khan, Ayesha, primary, Tran, Truc T., additional, Rios, Rafael, additional, Hanson, Blake, additional, Shropshire, William C., additional, Sun, Zhizeng, additional, Diaz, Lorena, additional, Dinh, An Q., additional, Wanger, Audrey, additional, Ostrosky-Zeichner, Luis, additional, Palzkill, Timothy, additional, Arias, Cesar A., additional, and Miller, William R., additional

Published
2019
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194. Integrated Analysis of Randomized Controlled Trials Evaluating Bortezomib + Lenalidomide + Dexamethasone or Bortezomib + Thalidomide + Dexamethasone Induction in Transplant-Eligible Newly Diagnosed Multiple Myeloma

Author

Rosinol Dachs, Laura, primary, Hebraud, Benjamin, additional, Oriol, Albert, additional, Colin, Anne-Laurène, additional, Rios, Rafael, additional, Hulin, Cyrille, additional, Blanchard, Maria Jesús, additional, Caillot, Denis, additional, Sureda, Anna, additional, Hernández, Miguel T, additional, Arnulf, Bertrand, additional, Mateos, Maria-Victoria, additional, Macro, Margaret, additional, San-Miguel, Jesús F, additional, Belhadj, Karim, additional, Lahuerta, Juan Jose, additional, Chen, Guang, additional, Garelik, M. Brigid, additional, Bladé, Joan, additional, Moreau, Philippe, additional, and Attal, Michel, additional

Published
2018
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196. Curativestategy (GEM-CESAR) for High-Risk Smoldering Myeloma (SMM): Carfilzomib, Lenalidomide and Dexamethasone (KRd) As Induction Followed By HDT-ASCT, Consolidation with Krd and Maintenance with Rd

Author

Mateos, Maria-Victoria, primary, Martínez-López, Joaquin, additional, Rodriguez Otero, Paula, additional, Ocio, Enrique M., additional, Gonzalez, Marta Sonia, additional, Oriol, Albert, additional, Gutierrez, Norma C., additional, Paiva, Bruno, additional, Rios, Rafael, additional, Rosinol, Laura, additional, Alvarez, Miguel Angel, additional, Calasanz, Maria Jose, additional, Bargay, Joan, additional, Gonzalez, Ana Pilar, additional, Alegre, Adrián, additional, Escalante, Fernando, additional, Martínez, Rafael, additional, Puig, Noemi, additional, De La Rubia, Javier, additional, Teruel, Ana Isabel, additional, Cedena, María Teresa, additional, De Arriba, Felipe, additional, Palomera, Luis, additional, Hernández, Miguel T, additional, Lopez Jimenez, Javier, additional, Martín, Jesús, additional, Piensa, Esther, additional, García Mateo, Aránzazu, additional, Gonzalez De La Calle, Veronica, additional, Bladé, Joan, additional, Lahuerta, Juan Jose, additional, and San-Miguel, Jesus F, additional

Published
2018
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197. Immunofixation (IF) in Urine Is Really Necessary to Define Complete Remission in Multiple Myeloma (MM)? a Subanalysis from the Pethema/GEM2012MENOS65 Phase III Clinical Trial

Author

Jiménez Ubieto, Ana, primary, Paiva, Bruno, additional, Martínez-López, Joaquin, additional, Rosinol, Laura, additional, Cedena Romero, Maria Teresa, additional, Calasanz, Maria Jose, additional, Puig, Noemi, additional, Garcia-Sanz, Ramon, additional, Gutierrez, Norma C., additional, Martin-Ramos, Maria Luisa, additional, Oriol, Albert, additional, Gonzalez Medina, José, additional, Fernandez, Rafael Alonso, additional, Blanchard, Jesús, additional, Rios, Rafael, additional, Martín, Jesús, additional, Martinez Martinez, Rafael, additional, Sarra, Josep, additional, Hernández, Miguel-Teodoro, additional, De La Rubia, Javier, additional, Krsnik, Isabel, additional, Moraleda, Jose Maria, additional, Palomera, Luis, additional, Mateos, Maria-Victoria, additional, San-Miguel, Jesús F, additional, Bladé, Joan, additional, and Lahuerta, Juan-José, additional

Published
2018
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198. Clinical Significance and Transcriptional Profiling of Persistent Minimal Residual Disease (MRD) in Multiple Myeloma (MM) Patients with Standard-Risk (SR) and High-Risk (HR) Cytogenetics

Author

Goicoechea, Ibai, primary, Puig, Noemi, additional, Cedena, María Teresa, additional, Cordon, Lourdes, additional, Vidriales, Maria-Belen, additional, Burgos, Leire, additional, Flores-Montero, Juan, additional, Gutierrez, Norma C., additional, Calasanz, Maria Jose, additional, Martin-Ramos, Maria Luisa, additional, Lara-Astiaso, David, additional, Vilas-Zornoza, Amaia, additional, Alignani, Diego, additional, Rodriguez, Idoia, additional, Sarvide, Sarai, additional, Garcia-Sanz, Ramon, additional, Martinez-Lopez, Joaquin, additional, Oriol, Albert, additional, Rios, Rafael, additional, Martín, Jesús, additional, Martínez, Rafael, additional, Sarra, Josep, additional, Hernández, Miguel T, additional, De La Rubia, Javier, additional, Krsnik, Isabel, additional, Moraleda, Jose Maria, additional, Palomera, Luis, additional, Bargay, Joan, additional, Orfao, Alberto, additional, Rosiñol, Laura, additional, Mateos, Maria-Victoria, additional, Lahuerta, Juan Jose, additional, Bladé, Joan, additional, San-Miguel, Jesus F, additional, and Paiva, Bruno, additional

Published
2018
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199. Detailed Phenotypic, Molecular and Functional Profiling of Myeloid Derived Suppressor Cells (MDSCs) in the Tumor Immune Microenvironment (TIME) of Multiple Myeloma (MM)

Author

Pérez Ruiz, Cristina, primary, Zabaleta, Aintzane, additional, Puig, Noemi, additional, Cedena, María Teresa, additional, Merino, Juana, additional, Alignani, Diego, additional, Garate, Sonia, additional, Goicoechea, Ibai, additional, Lara-Astiaso, David, additional, Sarvide, Sarai, additional, Botta, Cirino, additional, Martinez Lopez, Joaquin, additional, Oriol, Albert, additional, Rios, Rafael, additional, Rosinol, Laura, additional, Mateos, Maria-Victoria, additional, Lahuerta, Juan-José, additional, Bladé, Joan, additional, San-Miguel, Jesus F, additional, and Paiva, Bruno, additional

Published
2018
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200. Absence of Contribution to a Differential Outcome of the Stringent Complete Response IMWG Category Respect to the Conventional CR in Multiple Myeloma. a Validation Analysis Based on the Pethema/GEM2012MENOS65 Phase III Clinical Trial

Author

Jiménez Ubieto, Ana, primary, Martinez Lopez, Joaquin, additional, Rosinol, Laura, additional, Paiva, Bruno, additional, Cedena, María Teresa, additional, Puig, Noemi, additional, Calasanz, Maria Jose, additional, Gonzalez Medina, José, additional, Martin-Ramos, Maria Luisa, additional, Fernandez, Rafael Alonso, additional, Oriol, Albert, additional, Blanchard, Jesús, additional, Ocio, Enrique M., additional, De La Rubia, Javier, additional, Rios, Rafael, additional, Martín, Jesús, additional, Hernández, Miguel-Teodoro, additional, Krsnik, Isabel, additional, Moraleda, Jose Maria, additional, Palomera, Luis, additional, Bargay, Joan, additional, Mateos, Maria-Victoria, additional, San-Miguel, Jesús F, additional, Bladé, Joan, additional, and Lahuerta, Juan-José, additional

Published
2018
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