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1,237 results on '"Sakai, Yasuyuki"'

154. Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment

Author

Danoy, Mathieu, primary, Tauran, Yannick, additional, Poulain, Stephane, additional, Jellali, Rachid, additional, Bruce, Johanna, additional, Leduc, Marjorie, additional, Le Gall, Morgane, additional, Koui, Yuta, additional, Arakawa, Hiroshi, additional, Gilard, Francoise, additional, Gakiere, Bertrand, additional, Kato, Yukio, additional, Plessy, Charles, additional, Kido, Taketomo, additional, Miyajima, Atsushi, additional, Sakai, Yasuyuki, additional, and Leclerc, Eric, additional

Published
2021
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157. In vivo-Like Hierarchical Coculture of Hepatocytes, Hepatic Stellate Cells, and Sinusoidal Endothelial Cells for TGF-b-Induced Early Liver Fibrosis Studies in vitro

Author

Lau, Qiao You, Torizal, Fuad Gandhi, Shinohara, Marie, and Sakai, Yasuyuki

Subjects
automotive_engineering
Abstract

During chronic liver injury, inflammation leads to liver fibrosis— particularly due to the activation of hepatic stellate cells (HSCs). However, the involvement of inflammatory cytokines in HSC activation and the relationship among different liver cells is unclear. To examine their interactions, many in vitro liver models are performed in organoid or spheroid culture with random 3D structure, complicating analysis. Herein, we demonstrated the hierarchical coculture of primary rat hepatocytes with non-parenchymal cells such as the human-derived HSC line (LX-2) and liver sinusoidal endothelial cell line (TMNK-1). The cocultured tissue had high usability with simple operation of separating solid and liquid phases with improved liver functions such as albumin production and hepatic cytochrome P450 3A4 activity. We also studied the effects of stimulation by both oxygen tension and the key pro-fibrogenic cytokine, transforming growth factor beta (TGF-β), on HSC activation. Gene expression analysis revealed that lower oxygen tension and TGF-β1 stimulation enhanced collagen type I and alpha-smooth muscle actin expression from LX-2 cells in the hierarchical coculture. Therefore, this hierarchical in vitro cocultured liver tissue could provide an improved platform as a disease model for elucidating the interactions of various liver cell types and biochemical signals in future liver fibrogenesis studies.

Published
2020

158. Hierarchical coculture of hepatocytes, hepatic stellate cells, and sinusoidal endothelial cells for TGF-b-induced early liver fibrosis studies in vitro

Author

Lau, Qiao You, Torizal, Fuad Gandhi, Shinohara, Marie, and Sakai, Yasuyuki

Subjects
automotive_engineering
Abstract

During chronic liver injury, inflammation leads to liver fibrosis— particularly due to the activation of hepatic stellate cells (HSCs). However, the involvement of inflammatory cytokines in HSC activation and the relationship between each type of liver cells is still unclear. To examine their interactions, many existing in vitro liver models were performed in 3D organoid or spheroid culture with random 3D structure, which complicates analysis. Herein, we demonstrated the hierarchical coculture of primary rat hepatocytes with non-parenchymal cells such as the human-derived HSC line (LX-2) and the human-derived liver sinusoidal endothelial cell line (TMNK-1). The cocultured tissue had high usability with simple operation of separating solid and liquid phases with improved liver functions such as albumin production and hepatic cytochrome P450 3A4 activity. We also studied the effects of stimulation by both oxygen tension and the key pro-fibrogenic cytokine, transforming growth factor beta (TGF-β), on HSC activation. Gene expression analysis revealed that lower oxygen tension and TGF-β1 stimulation enhanced collagen type I and alpha-smooth muscle actin expression from LX-2 cells in the hierarchical coculture after TGF-β1 stimulation. Therefore, this hierarchical in vitro cocultured liver tissue could provide an improved platform as a disease model for elucidating the interactions of various liver cell types and biochemical signals in future liver fibrogenesis studies.

Published
2020

173. Fabrication of a Porous Three-Dimensional Scaffold with Interconnected Flow Channels: Co-Cultured Liver Cells and In Vitro Hemocompatibility Assessment

Author

Li, Muxin, primary, Khadim, Rubina Rahaman, additional, Nagayama, Mitsuru, additional, Shinohara, Marie, additional, Inamura, Kousuke, additional, Danoy, Mathieu, additional, Nishikawa, Masaki, additional, Furukawa, Katsuko, additional, Sakai, Yasuyuki, additional, and Niino, Toshiki, additional

Published
2021
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174. Multi‐omics analysis of hiPSCs‐derived HLCs matured on‐chip revealed patterns typical of liver regeneration

Author

Danoy, Mathieu, primary, Tauran, Yannick, additional, Poulain, Stéphane, additional, Jellali, Rachid, additional, Bruce, Johanna, additional, Leduc, Marjorie, additional, Le Gall, Morgane, additional, Gilard, Francoise, additional, Kido, Taketomo, additional, Arakawa, Hiroshi, additional, Araya, Karin, additional, Mori, Daiki, additional, Kato, Yukio, additional, Kusuhara, Hiroyuki, additional, Plessy, Charles, additional, Miyajima, Atsushi, additional, Sakai, Yasuyuki, additional, and Leclerc, Eric, additional

Published
2021
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175. Sessile Liquid Marbles: Sessile Liquid Marbles with Embedded Hydrogels as Bioreactors for Three‐Dimensional Cell Culture (Adv. Biology 2/2021)

Author

Vadivelu, Raja, primary, Kashaninejad, Navid, additional, Nikmaneshi, Mohammad Reza, additional, Khadim, Rubina Rahaman, additional, Salehi, Seyedeh Sarah, additional, Ramulu, Naveen Chintala, additional, Sakai, Yasuyuki, additional, Nishikawa, Masaki, additional, Firoozabadi, Bahar, additional, and Nguyen, Nam‐Trung, additional

Published
2021
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186. Abstract of Poster Presentation

Author

Uchida, Hiroshi, Maruyama, Tetsuo, Sugiura, Jkuko, Kajitani, Takashi, Arase, Touru, Ono, Masanori, Nagashima, Takashi, Masuda, Hirotaka, Asada, Hironori, Yoshimura, Yasunori, Saito, Soichiro, Ghosh, Mila, Morita, Keiko, Miwa, Masanao, Hirano, Takashi, Todoroki, Takeshi, Kanao, Kento, Ohya, Mototsugu, Murai, Masaru, Ohnuki, Junko, Khor, Lee Wee, Kamiyama, Naoya, Tomioka, Nobumoto, Nakagawa, Takahito, Takahashi, Masato, Todo, Satoru, Kusakabe, Moriaki, Aotsuka, Satoshi, Inoue, Jun, Matsuba, Kyouichi, Hashimoto, Hisashi, Isonishi, Seiji, Yasuda, Makoto, Ishikawa, Hiroshi, Ueda, Masatsugu, Terai, Yoshito, Yamaguchi, Hiroyuki, Ueki, Minoru, Uchinokura, Shunro, Miyata, Shiro, Fukushima, Tsuyoshi, Itoh, Hiroshi, Nakano, Shinichi, Wakisaka, Shinichiro, Kataoka, Hiroaki, Ohi, Satoshi, Tabei, Isao, Hirabayashi, Tetsuya, Ninomiya, Kozou, Ichikawa, Ayumi, Ogata, Tomomi, Tachibana, Toshiyuki, Sato, Kahei, Yanaga, Katsuhiko, Tamagawa, Tomoharu, Iguchi, Megumi, Tokieda, Yuko, Ishiwata, Isamu, Ono, Isao, Kiguchi, Kazushige, Ishiwata, Chieko, Ishiwata, Emiko, Soma, Masayuki, Nakamura, Hiroko, Mizuno, Jinji, Fueta, Youichi, Kamakura, Hirokazu, Murayama, Yoshinobu, Omata, Sadao, Akaishi, Kazuyuki, Inui, Hiroaki, Mizuno, Jinii, Ostrovidov, Serge, Sakai, Yasuyuki, Fujii, Teruo, Watanabe, Natsumi, Hirayma, Kazuhiro, Kuriki, Eiko, Kobayashi, Mamoru, Yakuwa, Takushi, Okamoto, Naoki, Sato, Yorino, Tanaka, Fumi, Kazami, Ai, Hasegawa, Hisataka, Tanaka, Naoki, Araki, Yasuyuki, Yoshizawa, Midori, Araki, Yasuhisa, Sakaguchi, Eriko, Tomita, Kouichi, Ninomiya, Kozo, Ishida, Yuichi, Tachibana, Toshiaki, Tsuboi, Kumiko, Ishikawa, Mayumi, Ueshiba, Hajime, Kitahara, Shinzo, Yoshino, Gen, Tamagawa, Tomoharu, Ishiwata, Samu, Miharada, Kenichi, Sudo, Kazuhiro, Nakamura, Yukio, Li, Qiang, Ryu, Takashi, Azuma, Keiichi, Hosaka, Naoki, Ikehara, Susumu, Kawamoto, Keiji, Ono, Masanori, Hamatani, Toshio, Okano, Hideyuki, Matsuzaki, Yumi, Yamamoto, Matsuo, Sakoda, Kenji, Negishi, Yoichi, Sekiya, Hideki, Sakiyama, Yusuke, Suzuki, Kazunari, Yano, Tomoya, Fukawa, Yukiko, Node, Koichi, Izumi, Yuichi, Kobayashi, Makoto, Takagi, Takamasa, Takahashi, Noriko, Mitsui, Masashi, Murayama, Reiichiro, Moritaka, Shunichiro, Tsurumi, Ayuko, Hayashi, Iyou, Hayashi, Yukie, Okamatsu, Yoshimasa, Miharada, Ken-ichi, Hiroyama, Takashi, Fujioka, Tsuyoshi, Nagasawa, Toshiro, Suzuki, Nao, Orikawa, Kimiko, Tamada, Yutaka, Suzuki, Atsushi, Susumu, Nobuyuki, Tsukazaki, Katsumi, Mukai, Makio, Kojima-Aikawa, Kyoko, Ishida, Isao, Aoki, Daisuke, Kobayashi, Yoichi, Takahashi, Noriyuki, Ohara, Tatsuru, Okuda, Yoshiko, Sato, Sojiro, Ishizuka, Bunpei, Sato, Akinori, Ito, Keiichi, Ooya, Mototsugu, Asano, Takako, Sumitomo, Makoto, Horiguchi, Yutaka, Asano, Tomohiko, Hayakawa, Masamichi, Sasaki, Naoki, Kita, Tsunekazu, Okamoto, Sanshiro, Takano, Masashi, Kudoh, Kazuya, Furuya, Kenichi, Kikuchi, Yoshihiro, Nariai, Koichi, Yoshikawa, Tetsuya, Mitsunaga, Makoto, Sumi, Makoto, Yumoto, Yoko, Mabashi, Yasuo, Namiki, Yoshihisa, Tsubota, Akihito, Fujise, Kiyotaka, Takahashi, Hiroshi, Harada, Hideki, Suzu, Shinya, Ito, Takaaki, Okada, Seiji, Suzuki, Nagazumi, Ueda, Kazu, Yamada, Kyosuke, Tanaka, Tadao, Kondo, Kan, Shimada, Yutaka, Nakagami, Yoshihiro, Aoyagi, Teiichiro, Gondo, Tatsuo, Sakamoto, Noboru, Ohno, Yoshio, Koga, Shouji, Namiki, Kazunori, Yoshioka, Kunihiko, Ohori, Makoto, Hatano, Tadashi, Tachibana, Masaaki, Kobayashi, Yoichi, Watanabe, Mari, Wada, Yasuna, and Mizuhara, Hiroshi

Published
2005
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188. Development of microfluidic device for electrical/physical characterization of single cell

Author

Cho, Younghak H., Yamamoto, Takatoki, Sakai, Yasuyuki, Fujii, Teruo, and Kim, Beomjoon

Subjects
Impedance, Bioelectric -- Research, Microelectromechanical systems -- Research, Erythrocytes -- Observations, Engineering and manufacturing industries, Science and technology
Abstract

A novel device with microchannels for flowing cells and twin microcantilever arrays for measuring the electrical impedance of a single cell is proposed. The fabrication process is demonstrated and the twin microcantilever arrays have been successfully fabricated. In our research, we measured the electrical impedance for normal and abnormal red blood cell over the frequency range from 1 Hz to 10 MHz. From the electrical impedance experiment of normal and abnormal red blood cell, it was examined that the electrical impedance between normal and abnormal red blood cells was significantly different in magnitude and phase shift. In this paper, we show that the normal cell can be taken apart from the abnormal cell by electrical impedance measurement. Therefore, it is expected that the applicability of this technology can be used in cellular studies such as cell sorting, counting or membrane biophysical characterization. [1619] Index Terms--Electrical impedance, red blood cell, twin microcantilever array.

Published
2006

190. Amperometric biosensing systems based on motility and gravitaxis of flagellate algae for aquatic risk assessment

Author

Shitanda, Isao, Takada, Kazutake, Sakai, Yasuyuki, and Tatsuma, Tetsu

Subjects
Biosensors -- Research, Chlamydomonas -- Research, Chemistry
Abstract

Electrochemical biosensing systems for toxic substances were developed on the basis of motility and negative gravitaxis of the unicellular flagellate Chlamydomonas reinhardtii. Changes in the flagellar movement of the flagellates in response to three toxic chemicals, toluene, copper(II) sulfate, and nickel(II) chloride, were monitored as changes in the redox currents for a coexisiting redox marker. The gravitaxis-based flagellate biosensing system was more sensitive to toluene than the motility-based system. A thin-layer flagellate biosensor was also developed. In comparison with the conventional algal biosensors monitoring the photosynthetic activity, the gravitaxis-based thin-layer sensor was more sensitive by more than 1 order of magnitude.

Published
2005

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