151. In vivo MRI tracking of exogenous monocytes/macrophages targeting brain tumors in a rat model of glioma
- Author
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Emmanuel L. Barbier, Simon Roussel, Chantal Rémy, Christoph Segebarth, Samuel Valable, Edwige Petit, Myriam Bernaudin, Grenoble Institut des Neurosciences ( GIN ), Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Neurodégénérescence : modèles et stratégies thérapeutiques ( NMST ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), The authors wish to thank the 'Association pour la Recherche sur le Cancer', the 'Region Rhône-Alpes', and the 'Cancéropôle Lyon Auvergne Rhône-Alpes' for their financial support., Neurodégénérescence : modèles et stratégies thérapeutiques (NMST), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Neuroimagerie Fonctionnelle et Metabolique, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Grenoble Institut des Neurosciences (GIN), and Dojat, Michel
- Subjects
Male ,Pathology ,MESH: Monocytes ,Ferric Compounds ,Monocytes ,MESH: Magnetic Resonance Imaging ,030218 nuclear medicine & medical imaging ,[ SDV.IB.MN ] Life Sciences [q-bio]/Bioengineering/Nuclear medicine ,[ SDV.CAN ] Life Sciences [q-bio]/Cancer ,MESH: Glioma ,Stereotaxic Techniques ,0302 clinical medicine ,Lectins ,MESH : Particle Size ,MESH : Injections, Intravenous ,Medicine ,MESH: Animals ,Coloring Agents ,MESH : Macrophages ,medicine.diagnostic_test ,Brain Neoplasms ,MESH : Rats ,Brain ,Glioma ,MESH : Ferric Compounds ,Immunohistochemistry ,Magnetic Resonance Imaging ,3. Good health ,Neurology ,Data Interpretation, Statistical ,Injections, Intravenous ,MESH: Brain Neoplasms ,Stereotactic injection ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,MESH: Ferric Compounds ,medicine.medical_specialty ,MESH: Rats ,Cognitive Neuroscience ,Iron ,MESH : Male ,Rat model ,Green Fluorescent Proteins ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,[SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,MESH : Rats, Wistar ,Fluorescence ,[SDV.IB.MN] Life Sciences [q-bio]/Bioengineering/Nuclear medicine ,C6 cells ,03 medical and health sciences ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,In vivo ,Cell Line, Tumor ,MESH : Magnetic Resonance Imaging ,Monocytes macrophages ,Animals ,MESH: Particle Size ,Rats, Wistar ,Particle Size ,MESH : Brain Neoplasms ,Cell Proliferation ,business.industry ,[ SDV.BC ] Life Sciences [q-bio]/Cellular Biology ,Leukemia P388 ,Macrophages ,MESH: Macrophages ,Magnetic resonance imaging ,Histology ,MESH: Rats, Wistar ,medicine.disease ,MESH: Injections, Intravenous ,MESH : Disease Models, Animal ,MESH: Male ,Rats ,Disease Models, Animal ,MESH : Monocytes ,MESH : Glioma ,[ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,MESH : Animals ,MESH: Disease Models, Animal ,business ,030217 neurology & neurosurgery ,Ferrocyanides - Abstract
International audience; This study has shown that murine monocytes/macrophages (Mo/Ma) can be labeled simply and efficiently with large, green-fluorescent, micrometer-sized particles of iron-oxide (MPIO). Neither size nor proliferation rate of the Mo/Ma is significantly affected by this labeling. The labeled Mo/Ma have been administered intravenously to rats that had developed a glioma following stereotactic injection of C6 cells. The labeled Mo/Ma were shown to target the brain tumors, a process that could be monitored non-invasively using T2*-weighted MRI. MRI observations were confirmed by Prussian blue staining, lectin staining and fluorescence histology. Overall, the results of this study suggest that the use of Mo/Ma may be envisaged in the clinic for vectorizing therapeutic agents toward gliomas.
- Published
- 2008