151. Effects of Volatile Components of Modified Wendan Decoction on Improving the Behavior of Autistic Rats.
- Author
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Shengnan GUO, Jinqing LU, Lijuan WAN, Junjie XU, Qiang LI, and Junlong CAI
- Subjects
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LABORATORY rats , *INTRAPERITONEAL injections , *SALINE solutions , *DISTILLED water , *DISTILLATION , *SEIZURES (Medicine) , *PREVENTION - Abstract
[Objective] To research the effects of volatile components of Modified Wendan Decoction on improving the behavior of autistic rats. [Methods] Pregnant rats were randomly divided into two groups. On 12.5 d after pregnancy, intraperitoneal injection of 600 mg/kg VPA was carried out so as to establish autistic rats model. In control group, the same volume of 0.85% normal saline (NS) was intraperitoneal injected. The auxanology, behavior and morphology identification of rats were carried out. A total of 60 autistic rats and 20 rats in NS group were randomly collected on the 35th day after pregnancy. And autistic rats were randomly divided into distilled water group (DW), Modified Wendan Decoction group (VPA-1) and Modified Wendan Decoction and volatile oil group (VPA-2). There were 20 rats in each group. The volatile components of Modified Wendan Decoction were analyzed by steam distiilation method (SD) and GC-MS. [Results] VPA-1 group improved the behavior of autistic rats in certain degrees, but the effects were poorer than VPA-2 group. A total of 71 volatile components were detected. Among them, α-asarone, D-limonene and eugenol had relatively high content, accounting for 99.89% of the total volatile components. [Results] The volatile oils in Modified Wendan Decoction enhanced the therapeutic effects of autism. Literatures showed that α-asarone, D-limonene, eugenol and other volatile components adjusted the central nervous system, improved cerebral function, had the functions of anticonvulsion, anti-epileptic and diazepam, and improved the symptoms of autism. This research provided methodological basis for the analysis of volatile components in Modified Wendan Decoction, and provided references for the medicinal efficacy research on curing autism. [ABSTRACT FROM AUTHOR]
- Published
- 2015