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152 results on '"Takehisa Dewa"'

151. Reconstitution and Organization of Photosynthetic Antenna Protein Complex Bearing Functional Hydrophilic Domains.

Author

Shunsuke Sakai, Akito Hiro, Ayumi Sumino, Toshihisa Mizuno, Toshiki Tanaka, Hideki Hashimoto, Takehisa Dewa, and Mamoru Nango

Abstract

Bacterial photosynthetic antenna polypeptide (LH1-α) was synthesized as a water-soluble fusion protein with maltosebinding protein (MBP) and a His-tag portion (MBPrubα-YH) using an E. coli expression system. Reconstitution experiments indicated that LH1-type complexes with pigments were successfully formed, regardless of the presence of a large hydrophilic MBP portion. The reconstituted complex was immobilized onto a Au electrode via a His-tagNiNTA interaction. When the complex was incorporated into a planar lipid bilayer, protruding MBP portions were clearly observed by AFM. [ABSTRACT FROM AUTHOR]

Published
2011
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152. Ultrafast energy transfer in LH2 photosynthetic antenna conjugated with artificial fluorescent dyes

Author

Tetsuro Katayama, Hiroshi Miyasaka, Yutaka Nagasawa, Takehisa Dewa, Yusuke Yoneda, Tomoyasu Noji, and Naoto Mizutani

Subjects
biology, Absorption spectroscopy, Chemistry, business.industry, Conjugated system, Photochemistry, biology.organism_classification, Purple bacteria, Fluorescence, Femtosecond, Ultrafast laser spectroscopy, Optoelectronics, Antenna (radio), business, Spectroscopy
Abstract

Femtosecond transient absorption spectroscopy was carried out for energy transfer in photosynthetic purple bacteria LH2 antenna complex conjugated with artificial fluorescent dyes. Time constants of 3 ps and 18 ps were obtained by global analysis.

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