161 results on '"Watanabe, Sadayoshi"'
Search Results
152. Synthesis of a naphthalene diimide derivative having four ferrocene moieties as an electrochemical DNA hybridization indicator
- Author
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Sato, Shinobu, Tsueda, Masato, Watanabe, Sadayoshi, Ohtsuka, Keiichi, and Takenaka, Shigeori
- Abstract
A new naphthalene diimide derivative carrying four ferrocene moieties, F
4 ND, was synthesized by the reaction of trimethylammoniummethylferrocene with the terminal amino moieties of two imide substituents of naphthalene diimide. Spectrophotometric binding studies of F4 ND with calf thymus DNA showed its high affinity of 2 × 105 M−1 in 10 mM MES buffer and 1 mM EDTA (pH 6.25) containing 0.10 M NaCl. The Osteryoung square wave voltammetry (SWV) of a DNA probe-immobilized electrode before and after hybridization with target DNA gave a larger peak current in an electrolyte containing F4 ND than that containing FND carrying two ferrocene moieties. The current peak difference between mismatched and fully matched DNA was also greater with F4 ND than that with FND.- Published
- 2008
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153. Further stabilization of the double stranded DNA complex by naphthalene diimide derivative having 2,2-dipicolylamine-zinc complex
- Author
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Watanabe, Sadayoshi, Ohtsuka, Keiichi, and Takenaka, Shigeori
- Abstract
Designed naphthalene diimide derivative having 2,2-dipicolylamine (Dpa), NDI-Dpa, could form a Zn2+ complex and bind to calf thymus DNA with 10-times higher stabilization than that of NDI-Dpa. The obtained SDS-driven dissociation rate constant was 1.0 s−1, which is smaller than that of NDI-Dpa. These results suggested that NDI-Dpa-Zn can bind to double stranded DNA through threading intercalation and its complex was stabilized by the interaction of Dpa-Zn parts of NDI-Dpa-Zn and phosphodiester parts of dsDNA.
- Published
- 2007
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154. Abstract T P96.
- Author
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Miyata, Haruka, Nakahara, Ichiro, Matsumoto, Shoji, Ohta, Tsuyoshi, Fukushima, Yutaka, Ishibashi, Ryota, Gomi, Masanori, Nishi, Hidehisa, and Watanabe, Sadayoshi
- Published
- 2014
155. Glioependymal cyst in the medulla oblongata - A case report.
- Author
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Abdulateef AA, Morita S, Hoz SS, Atallah O, Numazawa S, Ito Y, Watanabe S, and Mori K
- Abstract
Background: Glioependymal cysts (GECs) are rare benign lesions that can be found anywhere along the neuroaxis, with most of the reports denoting supratentorial location. Here, we introduce a rare case of successfully treated glioependymal cysts lying in an uncommon location, namely medulla oblongata., Case Description: A 69-year-old lady presented with progressive unsteadiness and swallowing disturbances, and brain magnetic resonance imaging showed a dorsally located lesion within the medulla oblongata; based on the presentation and radiological features, surgical intervention was deemed mandatory. The suboccipital midline approach was used to perform marsupialization of the cyst with shunting through a syringosubarachnoid shunt to prevent future recurrence, and the patient outcome was improved., Conclusion: Medulla Oblongata's location for glioependymal cysts proposed unique diagnostic and operative challenges that may require highlighting for practicing neurosurgeons., Competing Interests: There are no conflicts of interest., (Copyright: © 2023 Surgical Neurology International.)
- Published
- 2023
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156. Validation of Efficacy and Safety of TachoSil ® Tissue Sealant for Vessel Transposition in Microvascular Decompression.
- Author
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Mori K, Otani N, Toyooka T, Morita S, Numazawa S, Wada K, and Watanabe S
- Subjects
- Humans, Follow-Up Studies, Retrospective Studies, Reproducibility of Results, Microvascular Decompression Surgery methods, Hemifacial Spasm surgery, Hemifacial Spasm etiology, Trigeminal Neuralgia surgery, Trigeminal Neuralgia etiology
- Abstract
Background and Objectives: Use of TachoSil ® as the transposition material of microvascular decompression (MVD) for hemifacial spasm (HFS) and trigeminal neuralgia (TN) is easy and safe to perform, but the efficacy and safety of this technique are unknown. This study attempted to validate the efficacy and safety of TachoSil ® as a transposition material of MVD., Methods: A retrospective study of the surgical results and complications of 63 patients (35 HFS and 28 TN) treated by the TachoSil ® technique between January 2011 and December 2021 was conducted. The efficacy of the treatment was evaluated by Kaplan-Meier survival analysis. Magnetic resonance imaging follow-up study was performed to detect any adverse events including a mass formation., Results: The rate of complete disappearance of HFS was 91.4% at 1 year and estimated to be 85.7% after a 10-year follow-up. The rate of no pain without medication for TN was 85.4% at 1 year and estimated to be 69.0% after a 9-year follow-up. These surgical results are comparable with those previously reported. Flaking of TachoSil ® releasing the offending artery was only recognized in one case (1.6%). Therefore, TachoSil ® can be considered as an effective transposition material for MVD. TachoSil ® did not increase the rate of acute and subacute adverse events such as inflammation and delayed facial palsy. Magnetic resonance imaging follow-up identified no abnormalities including mass that suggested granuloma formation., Conclusion: The efficacy of the TachoSil ® technique for HFS and TN and the reliability of TachoSil ® as an adhesive material in MVD were verified. No adverse events associated with TachoSil ® use in MVD were found. We conclude that the TachoSil ® technique has relatively long efficacy and safety for MVD., (Copyright © Congress of Neurological Surgeons 2023. All rights reserved.)
- Published
- 2023
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157. Factors influencing early obliteration during flow diverter treatment of cerebral aneurysms: Establishment of an early obliteration inhibition score.
- Author
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Hasebe A, Nakahara I, Matsumoto S, Morioka J, Tanabe J, Watanabe S, Suyama K, Ishihara T, and Hirose Y
- Abstract
Objective: This retrospective study aimed to investigate factors associated with inhibition of early aneurysm obliteration after flow diverter (FD) treatment. We also created the early obliteration inhibition (EOI) score for pre-operative evaluation., Methods: We examined 110 cerebral aneurysms in 104 patients who underwent FD treatment. The following parameters were investigated: age, sex, symptoms, aneurysm location and type, maximum aneurysm diameter, parent vessel diameter, neck diameter, and dome-neck ratio. We also noted aneurysm location relative to the curvature of the parent artery and any branches arising from the aneurysm dome. Procedural factors such as FD diameter and length, number of FDs placed, type of FD, and use of adjunctive coiling were also investigated. Aneurysm obliteration was evaluated using digital subtraction angiography 3 months after the procedure. Adequate obliteration was defined as grade C or D on the O'Kelly-Marotta scale., Results: The following factors inhibited early obliteration: 1) extradural location, 2) saccular aneurysm, 3) aneurysm neck located at the outer convexity of the parent artery, and 4) arterial branch arising from the aneurysm dome. Odds ratios were used to create an EOI score. Receiver operating characteristic curve analysis showed that the optimal cut-off EOI score for adequate obliteration was 1.5 (area under the curve, 0.81; 95% confidence interval, 0.73-0.9; sensitivity, 0.9; specificity, 0.57)., Conclusion: The EOI score, which is based on factors that inhibit early obliteration, may predict early treatment outcomes of FD placement., Competing Interests: One of the authors (I.N.) is a technical advisor for the Terumo Corporation and has a financial relationship with the company. The other authors have no conflicts of interest to declare.
- Published
- 2023
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158. Delays in initial workflow cause delayed initiation of mechanical thrombectomy in patients with in-hospital ischemic stroke.
- Author
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Suyama K, Matsumoto S, Nakahara I, Suyama Y, Morioka J, Hasebe A, Tanabe J, Watanabe S, Kuwahara K, and Hirose Y
- Abstract
Objectives: The benefit of mechanical thrombectomy for acute ischemic stroke is highly time dependent. However, time to treatment is longer for in-hospital stroke patients than community-onset stroke patients. This study aimed to clarify the cause of this difference., Methods: A retrospective single-center study was performed to analyze patients with large vessel occlusion who underwent mechanical thrombectomy between January 2017 and December 2019. Patients were divided into in-hospital stroke and community-onset stroke groups. Clinical characteristics and treatment time intervals were compared between groups., Results: One hundred four patients were analyzed: 17 with in-hospital stroke and 87 with community-onset stroke. Patient characteristics did not significantly differ between groups. Median door (stroke recognition)-to-computed tomography time (36 min vs. 14 min, P<0.01) and door-to-puncture time (135 min vs. 117 min, P=0.02) were significantly longer in the in-hospital stroke group than the community-onset stroke group. However, median computed tomography-to-puncture time (104 min vs. 104 min, P=0.47) and puncture-to-reperfusion time (53 min vs. 38 min, P=0.17) did not significantly differ., Conclusions: Longer door-to-puncture time in in-hospital stroke patients was mostly caused by longer door-to-computed tomography time, which is the initial part of the workflow. An in-hospital stroke protocol that places importance on early stroke specialist consultation and prompt transportation to the computed tomography scanner might hasten treatment and improve outcomes in patients with in-hospital stroke., Competing Interests: The authors declare no conflicts of interest.
- Published
- 2022
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159. [A Case of Bilateral Trigeminal Neuralgia and the Possible Onset Mechanisms Based on the Operative Findings].
- Author
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Tanaka M, Toyooka T, Matsuzaki T, Yamakawa K, Akasu I, Kitagawa R, Sakai J, Numazawa S, Itoh Y, Watanabe S, Otani N, Wada K, and Mori K
- Subjects
- Cerebellopontine Angle, Dura Mater, Female, Humans, Middle Aged, Pain, Treatment Outcome, Microvascular Decompression Surgery, Trigeminal Neuralgia surgery
- Abstract
Several cases of bilateral trigeminal neuralgia(TN)have been reported;however, the possible onset mechanism has rarely been discussed. We encountered a case of bilateral TN occurring in two stages. A 64-year-old woman presented with left TN. Magnetic resonance imaging showed the transverse pontine vein adhering to the left trigeminal root and superior cerebellar artery adhering to the right trigeminal root;however, no symptoms were noted. Immediately after microvascular decompression(MVD)on the left side, TN disappeared completely. However, 2 years postoperatively, the patient presented with right TN. The second MVD surgery revealed that the right cerebellar surface severely adhered to the dura mater, particularly under the surface of the tentorium. The arachnoid membrane at the cerebellopontine angle was slightly adhered. The patient was completely free from pain after the second MVD. The intraoperative findings suggested that the brain stem may have shifted and the cerebellopontine cistern may have narrowed because of cerebellar adhesion to the surrounding structures and arachnoid adhesion. We speculate that such structural changes in the posterior fossa after the first operation may have caused the asymptomatic vascular adhesion to change into the symptomatic offending adhesion over time.
- Published
- 2020
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160. Platelet reactivity and hemorrhage risk in neurointerventional procedures under dual antiplatelet therapy.
- Author
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Nishi H, Nakahara I, Matsumoto S, Hashimoto T, Ohta T, Sadamasa N, Ishibashi R, Gomi M, Saka M, Miyata H, Watanabe S, Okata T, Sonoda K, Kouge J, Ishii A, Nagata I, and Kira J
- Subjects
- Aged, Aspirin adverse effects, Aspirin therapeutic use, Clopidogrel, Drug Therapy, Combination, Female, Humans, Intracranial Aneurysm drug therapy, Male, Middle Aged, Platelet Aggregation Inhibitors therapeutic use, Platelet Function Tests, Prospective Studies, ROC Curve, Risk, Ticlopidine adverse effects, Ticlopidine analogs & derivatives, Ticlopidine therapeutic use, Treatment Outcome, Carotid Stenosis therapy, Embolization, Therapeutic, Hemorrhage chemically induced, Intracranial Aneurysm therapy, Platelet Activation drug effects, Platelet Aggregation Inhibitors adverse effects, Stents
- Abstract
Background and Purpose: Hemorrhagic complications during neurointerventional procedures have various etiologies and can result in severe morbidity and mortality. This study investigated the possible association between low platelet reactivity measured by the VerifyNow assay and increased hemorrhagic complications during elective neurointervention under dual antiplatelet therapy., Methods: From May 2010 to April 2013 we recorded baseline characteristics, P2Y12 reaction units (PRU), and aspirin reaction units using VerifyNow. The primary endpoint was post-procedural hemorrhagic complications., Results: A total of 279 patients were enrolled and 31 major hemorrhagic complications (11.1%) were identified. From receiver-operating characteristic curve analysis, PRU values could discriminate between patients with and without major hemorrhagic complications (area under the curve 0.63). Aspirin reaction unit values had no association with the primary outcome. The optimal cut-off for the primary outcome (PRU ≤175) was used to identify the low platelet reactivity group. The incidence of hemorrhagic complications was 20.0% in this group and 8.9% in the non-low platelet reactivity group. Multivariate analysis identified low platelet reactivity as an independent predictor for hemorrhagic complications., Conclusions: The risk of hemorrhagic complications during elective neurointervention including cerebral aneurysm coil embolization and carotid artery stenting under dual antiplatelet therapy is associated with the response to clopidogrel but not to aspirin. A PRU value of ≤175 discriminates between patients with and without hemorrhagic complications. Future prospective studies are required to validate whether a specific PRU value around 170-180 is predictive of hemorrhagic complications., (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/)
- Published
- 2016
- Full Text
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161. Synthesis of naphthalenediimide having two beta-cyclodextrins at both of its substituent termini.
- Author
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Ohtsuka K, Sato S, Watanabe S, Komizo K, and Takenaka S
- Subjects
- Chromatography, High Pressure Liquid, Cyclodextrins chemistry, DNA chemistry, Imides chemistry, Intercalating Agents chemistry, Kinetics, Cyclodextrins chemical synthesis, Imides chemical synthesis, Intercalating Agents chemical synthesis, beta-Cyclodextrins chemistry
- Abstract
Naphthalenediimide (1) carrying two beta-cyclodextrins (beta CDs) was successfully synthesized by click chemistry between pentynylnaphthalenediimide and azido beta CD. Absorption spectra of 1 showed hypochromic and red shifts upon addition of sonicated calf thymus DNA as a double stranded DNA. Kinetic studies showed its slow association with and dissociation from calf thymus DNA. These results suggested that 1 can bind to double stranded DNA by the threading mode, where one of the beta CD is required to go through adjacent base pairs of double stranded DNA. If this is proven, beta CD is one of the largest groups ever reported which can go through a DNA duplex for threading.
- Published
- 2008
- Full Text
- View/download PDF
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