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Sugarcane, a vital cash crop, contributes significantly to the world’s sugar supply and raw materials for biofuel production, playing a significant role in the global sugar industry. However, sustainable productivity is severely hampered by biotic and abiotic stressors. Genetic engineering has been used to transfer useful genes into sugarcane plants to improve desirable traits and has emerged as a basic and applied research method to maintain growth and productivity under different adverse environmental conditions. However, the use of transgenic approaches remains contentious and requires rigorous experimental methods to address biosafety challenges. Clustered regularly interspaced short palindromic repeat (CRISPR) mediated genome editing technology is growing rapidly and may revolutionize sugarcane production. This review aims to explore innovative genetic engineering techniques and their successful application in developing sugarcane cultivars with enhanced resistance to biotic and abiotic stresses to produce superior sugarcane cultivars.

The first case of human infection caused by the yeast Candida haemulonii was reported in 1984 (1). Recent research has indicated that the previously recognized C. haemulonii species is actually [...]

为明确元宝枫种仁的营养成分，对元宝枫种仁含油率、粗蛋白质含量，元宝枫种仁粕中氨基酸组成及含量、矿质元素组成及元宝枫种仁油的总脂肪酸、Sn-2位脂肪酸组成及含量、生育酚含量进行测定。结果表明：元宝枫种仁的含油率为43.7%，粗蛋白质含量为35.13%；元宝枫种仁粕中含有17种氨基酸，其中7种人体必需氨基酸占氨基酸总量的33.78%，是理想的蛋白质资源；元宝枫种仁粕中含有丰富的矿质元素，常量元素中K含量为6 372.8 μg/g，Na含量仅为346.5 μg/g，属于高钾低钠食品，微量元素中Mn含量较高，为68.1 μg/g，且未检出重金属元素；元宝枫种仁油主要由12种脂肪酸组成，神经酸含量达到5.89%，以亚油酸和α-亚麻酸为主的多不饱和脂肪酸主要分布在Sn-2位；元宝枫种仁油中生育酚总量为1 230.70 mg/kg，以γ-生育酚含量最高，达到792.62 mg/kg。元宝枫种仁营养丰富，可作为神经酸、生育酚和氨基酸的重要来源，其油脂可作为富含神经酸的保健食用油。 To clarify the nutritional components of Acer truncatum seed kernel, the contents of oil and crude protein in Acer truncatum seed kernel, the amino acid composition and mineral element composition of Acer truncatum seed kernel meal, the composition of total fatty acids, Sn-2 fatty acids and tocopherol content in Acer truncatum seed kernel oil were analyzed. The results showed that the contents of oil and crude protein in Acer truncatum seed kernel were 43.7% and 35.13% respectively. Acer truncatum seed kernel meal contained 17 kinds of amino acids, of which 7 kinds of essential amino acids accounted for 33.78%, and it was an ideal protein resource. Acer truncatum seed kernel meal was rich in mineral elements, with K content in major elements of 6 372.8 μg/g, and Na content of 346.5 μg/g, belonging to high potassium and low sodium food. The Mn content in trace elements was higher(68.1 μg/g), and no heavy metal element was detected. Acer truncatum seed kernel oil was mainly composed of 12 kinds of fatty acids, and the content of nervonic acid reached 5.89%. Polyunsaturated fatty acids, mainly linoleic acid and α-linolenic acid, were mainly distributed in Sn-2 position. The total contents of tocopherols in Acer truncatum seed kernel oil was 1 230.70 mg/kg, in which γ- tocopherol content was the highest, reaching 792.62 mg/kg. Acer truncatum seed kernel is rich in nutrition and can be used as an important source of nervonic acid, tocopherol and amino acids. Acer truncatum seed kernel oil can be used as a health edible oil.

Ovarian cancer is the second leading cause of death in women with gynecological malignancy in China. Circular RNAs are a class of noncoding regulatory RNAs reported to be involved in cancer development and progression. Previous studies, including our own, have indicated that hsa_circ_0007444 is downregulated in ovarian cancer tissues. This study aims to elucidate the function and mechanism of hsa_circ_0007444 in ovarian cancer progression. The expression of hsa_circ_0007444 is determined by quantitative real-time PCR (qRT-PCR). Cell proliferation, invasion, migration and apoptosis are examined by cell counting-kit 8 (CCK-8), transwell and flow cytometry assays. Tumor growth and metastasis are assessed in vivo using Balb/c nude mouse xenograft model and tail vein injection model. And the mechanism of action of hsa_circ_0007444 is analysed by RNA-binding protein immunoprecipitation (RIP), luciferase reporter and rescue assays. hsa_circ_0007444 is downregulated in ovarian cancer tissues and cell lines compared with that in normal ovarian tissues and normal epithelial cell line. Gain- and loss-of-function results indicate that hsa_circ_0007444 inhibits cell proliferation, invasion, migration and increases cell apoptosis of ovarian cancer cells in vitro, and inhibits tumor growth and lung metastasis in vivo. Mechanistically, hsa_circ_0007444 can interact with AGO2 and sponge miR-23a-3p, thereby upregulating DICER1 expression, which is an important tumor suppressor in ovarian cancer. And miR-23a-3p mimics can rescue the inhibitory effect of hsa_circ_0007444 on ovarian cancer cell proliferation, invasion and migration. Therefore, hsa_circ_0007444 can inhibit ovarian cancer progression through the hsa_circ_0007444/miR-23a-3p/DICER1 axis.

This paper summarized the nursing management for a patient undergoing bedside continuous renal replacement therapy (CRRT) for acute exacerbation of chronic renal failure. Key issues of nursing including hemofiltration catheter maintenance, illness condition observation, psychological care and complications prevention and control. Clinical symptoms such as limb edema and dyspnea were successful relieved after implementation of CRRT and related nursing interventions, and patient had completed the following treatment. (本文回顾1例慢性肾衰竭急性加重期患者行床旁连续性肾脏替代治疗的效果, 总结护理经验, 包括血液滤过置管护理、病情观察、心理护理、并发症护理等。通过床旁连续性肾脏替代治疗治疗后患者水肿、喘憋等临床症状明显好转。)

BACKGROUND Uranium-series nuclides are one of the three major radioactive decay systems, which are suitable for studying various geological processes at different time scales. In addition, 87Sr/86Sr and 143Nd/144Nd isotopes (Sr-Nd isotopes for short) are two commonly used isotopes, for rock dating, chemical weathering assessment and tracing sediment sources. In this case, the combination of Sr-Nd-U isotopes can provide more comprehensively knowledge of the element cycle on the earth's surface and deepen our understanding of the sediment "Source to Sink"processes.Most previous studies involving the Sr-Nd-U isotopes have been established by separating Sr-Nd and U isotopes respectively. In this way, the digestion operation must be performed twice, one for separating Sr-Nd and the other for separating U. Alternatively, if only one sample is digested for measuring all three isotopes, between each element separation, the residue must be dried and dissolved in another solution so as to start a new column work. The former increases the amount of samples, which is not conducive to analysis of precious and trace samples; the latter adds additional drying operation, which is time consuming and increases the risk of sample loss and contamination. OBJECTIVES To establish a new Sr-Nd-U combined separation scheme. In this method, only one sample is dissolved, avoiding solution transfer between each separation, so as to reduce sample amount and improve the efficiency of separation and purification of Sr-Nd-U isotopes. METHODS A new chromatographic scheme of separating Sr-Nd-U with one sample digestion using a tandem column scheme is presented. Three columns were overlain sequentially to separate Sr in Sr Spec column, Nd in AG50W-X8 column and U in UTEVA column. 3mol/L HNO3 was used to pre-condition, load the sample, and rinse the matrix. After rinsing the matrix, the tandem column was separated to 3 independent columns to elute the target elements (Sr, Nd, U) respectively.As the connection sequence of different resin columns may interfere with the recovery of target elements, two different chromatographic schemes were compared. In Scheme 1, U column was placed on top of Sr column, while in Scheme 2 the positions of U column and Sr resin column were exchanged. In both schemes, the AG50W-X8 resin column was set at the bottom as the cationic resin can adsorb the most complex elements.All separated elution was tested for element concentration using inductively coupled plasma-mass spectrometry (ICP-MS). The basalt standard sample (BCR-2) was used to examine the behavior and recovery of each element in the separation procedure. RESULTS A total of 10 fractions were recovered from the tandem column scheme, among which fraction 1 represented the leachate recovered by loading samples and rinsing matrix from the tandem three columns. Fraction 2, 5 and 8 represented the leachate recovered by Sr Spec resin, AG50W-X8 resin and UTEVA resin respectively rinsing matrix after separation of the three columns. Fraction 3, 6 and 9 represented the leachate recovered by Sr, REE and U columns, which was Sr, Nd and U collection. Fraction 4, 7 and 10 represented the leachate from each resin recycle stage.In either Scheme 1 or Scheme 2, most matrix elements (high content of K, Ca, Na, Mg, Al, Fe, Ti and P and low content of Rb, Hf and Th) were mainly concentrated in fraction 1. The elution rate of Na, Ti, Rb and Hf was up to 99%. The elution rate of K and Ca was slightly lower at about 85% and the elution rate of Fe was about 56%. Sr was mainly concentrated in fraction 3, which contained only a small amount of P and Ba. Nd was mainly concentrated in fraction 6, which also contained both Sm and Ce. U was mainly concentrated in fraction 9, which only contained a very small amount of P and Pb. The column recovery was almost 99.9% for U, 90% for Sr and over 80% for Nd.The removal rate of major matrix elements (K, Ca, Na, Ba, Fe, Rb, etc.) exceeded 99%, which reduced interference with high-precision isotope analysis of Sr, Nd, and U. The recovery and purity of Sr, Nd, U were all quite high. A very small amount of P and Ba in fraction 3 had no interference with Sr isotopes (87Sr/86Sr). The Rb which was isomorphism of Sr was removed completely. With regard to Sm and Ce in fraction 6, previous studies had shown that 142Ce could not interfere with Nd isotope (143Nd/144Nd), and Sm could be further separated by Ln resin, so as not to affect Nd isotopic test. Fraction 9 contained nearly 100% U with no other elements.The sequence of resin column splicing is a crucial consideration which may impact the element separation. Hence, the position of Sr Spec column and UTEVA column was exchanged to compare the influence of different column sequences on eluting target elements. Both Scheme 1 and Scheme 2 can effectively wash off most of the matrix elements, and the target elements Sr, Nd and U can be efficiently adsorbed on the resin. There is no significant difference on target element separation between the two different column sequences. This indicates that Sr Spec and UTEVA resins do not interfere with each other on the target elements. CONCLUSIONS The new chromatographic scheme of separating Sr-Nd-U with one sample digestion using a tandem column scheme can be used to quickly and efficiently separate Sr, Nd and U elements from silicate rock samples. The recovery rate for U, Sr and Nd is 99.9%, 92.5% and 82.1%, respectively, which meet the requirements of subsequent isotope analysis. This Sr-Nd-U combined separation method can be used to reduce the sample consumption by about 50%, which is beneficial to the analysis of precious and trace samples. Meanwhile, as no solution transfer is needed between each column separation, this method can also save time for column work and increase the efficiency of chemical separation. A new idea for Sr-Nd-U multi-isotope separation is provided. If the recovery of Pb in the fraction 4 of this chromatographic scheme can be improved in further studies, the application of this new method may be expanded to more fields in the future.