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In this study, a myrosinase-producing strain named RJ35 was isolated from a rapeseed field soil, and it was identified by morphological and molecular biological methods. The myrosinase produced by strain RJ35 was purified by sequential ultrafiltration and gel filtration chromatography and its enzymatic properties were studied. Strain RJ35 was assigned to the genus Sphingobacter. The molecular mass of the myrosinase was about 100 kDa. The optimum pH and temperature of the myrosinase were 7.5 and 40 ℃, respectively. Moreover, metal ions had no significant activating effect on the myrosinase. The results of high performance liquid chromatography (HPLC) showed that this enzyme could hydrolyze radish seed extract to produce sulforaphane. Hydrolysis for 30 min under the optimum conditions resulted in a yield of sulforaphane of 5.95 mg/g (radish seed). These research results may lay an experimental foundation for the development of microbial myrosinase.

Objective: To assess the efficacy of Sanyrene liquid dressing (Urgo Medical) in preventing radiation dermatitis (RD) among patients with cancer after radiotherapy., Data Sources: The authors searched the China National Knowledge Infrastructure, SinoMed, WanFang Data, PubMed, Web of Science, EMBASE, and the Cochrane Library databases for articles published from inception to January 2021., Study Selection: The preliminary search identified 146 studies. After removing duplicates, applying exclusion criteria, and screening titles and abstracts, 19 studies met the inclusion criteria., Data Extraction: A standardized form was constructed to extract data from eligible studies. Two reviewers independently screened the literature, extracted data, and assessed the risk of bias of the included studies., Data Synthesis: The authors identified a total of 19 studies involving 1,508 patients that assessed the effectiveness of Sanyrene liquid dressing in preventing RD in patients with cancer after radiotherapy. The findings suggested that Sanyrene decreases the total incidence of RD (odds ratio [OR], 5.00; 95% CI, 2.77-9.03; P < .00001), as well as the incidence of RD grade 2 (OR, 0.55; 95% CI, 0.36-0.85; P = .007), grade 3 (OR, 0.22; 95% CI, 0.09-0.57; P = .002), and grade 4 (OR, 0.32; 95% CI, 0.13-0.78; P = .01). In addition, in comparison with controls, Sanyrene liquid dressing improves the cure rate (OR, 8.18; 95% CI, 4.03-16.60; P < .00001) and delays the occurrence of RD (mean difference, 3.69; 95% CI, 3.03-4.36; P < .00001)., Conclusions: Sanyrene liquid dressing can decrease both the total incidence of RD and the incidence of RD above grade 2. It also improves the cure rate and delays the occurrence of RD. Thus, Sanyrene may be a superior option for preventing RD after radiotherapy. However, the findings were assessed as moderate- to low-quality evidence and more high-quality trials are needed to support this result., (Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.)