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In this work, a novel, simple and label-free electrochemiluminescence (ECL) strategy was designed for a sensitive carcinoembryonic antigen (CEA) assay on the basis of ZnS–CdS nanoparticle (NP)-decorated molybdenum disulfide (MoS2) nanocomposites. Layered-MoS2 nanocomposites with a large surface area were prepared with a facile hydrothermal method and used as an electrode matrix for subsequent ZnS–CdS NP electrodeposition. The ZnS–CdS NP decorated MoS2 electrode shows a higher ECL intensity than an electrode with only ZnS–CdS electrodeposition. For the fabrication of the aptasensor, chitosan was coated on a ZnS–CdS/MoS2/glass carbon electrode for CEA aptamer immobilization through a covalent coupling method. The ECL aptasensor for CEA detection exhibits a linear range from 0.05 to 20 ng mL−1 (R = 0.9975) with a low detection limit of 0.031 ng mL−1, and presents satisfactory specificity against some possible interfering proteins. Moreover, the method was successfully applied for CEA determination in human serum with recoveries of 80–111%, showing great promise for application in practice.

Metal oxide photocatalysts have high chemical stability in aqueous solutions, but their photocatalytic activity is usually restricted by poor electron–hole separation. Although impurity doping has been demonstrated to be an effective way to tune photocatalytic properties, the mechanisms of enhanced photocatalytic activity remain ambiguous. Here we show that appropriately incorporated impurities can markedly improve the spatial carrier separation and photocatalytic activity. This concept is demonstrated by experimental and density functional theory studies on the effects of fluorine (F) and nitrogen (N) dopants on photocatalytic H2 evolution performance of m-ZrO2 nanocrystals. We found that F readily enters the lattice of m-ZrO2 to form F substitutional defect (FO) with low defect formation energy (DFE), but N has difficulty entering the lattice to form N substitutional defect (NO) unless N is codoped with F. The F doping doubled the photocatalytic activity of m-ZrO2 without reducing its bandgap, which is attributed to the enhanced spatial carrier separation caused by defect FO. The F–N codoping quadrupled the photocatalytic activity of m-ZrO2, with the enhanced photocatalytic activities attributed to the synergetic effect of the impurity-enhanced spatial carrier separation and the increased light absorption. We observed that F–N codoping enhances photocatalytic performance by affecting both separation and recombination of the carriers: (1) F–N codoping enhances spatiotemporal separation of electrons and holes, and (2) F–N codoping reduces spatial overlap between the electron and hole distributions and inhibits recombination of the carriers. Our findings may provide novel insights into designing high-performance photocatalysts.

Previous studies have revealed that some proteins in Bombyx mori larvae digestive juice show antiviral activity. Here, based on the label-free proteomics data, BmLipase member H-A (BmLHA) was identified as being involved in the response to BmNPV infection in B. mori larvae digestive juice. In the present study, a gene encoding the BmLHA protein in B. mori was characterized. The protein has an open reading fragment of 999 bp, encoding a predicted 332 amino acid residue-protein with a molecular weight of approximately 35.9 kDa. The phylogenetic analysis revealed that BmLHA shares a close genetic distance with Papilio xuthus Lipase member H-A. BmLHA was highly expressed in the middle part of the B. mori gut, and the expression level increased with instar rising in larvae. There was higher expression of BmLHA in A35 than in P50 strains, and it was upregulated in both A35 and P50 strains, following BmNPV infection. The expression level of VP39 decreased significantly in appropriate recombinant-BmLHA-treated groups compared with the PBS-treated group in B. mori larvae and BmN cells. Meanwhile, overexpression of BmLHA significantly reduced the infectivity of BmNPV in BmN cells. These results indicated that BmLHA did not have digestive function but had anti-BmNPV activity. Taken together, our work provides valuable data for the clarification of the molecular characterization BmLHA and supplements research on proteins of anti-BmNPV activity in B. mori.

Background P53 is the most frequently mutated gene in most tumour types, and the mutant p53 protein accumulates at high levels in tumours to promote tumour development and progression. Thus, targeting mutant p53 for degradation is one of the therapeutic strategies used to manage tumours that depend on mutant p53 for survival. Buxus alkaloids are traditionally used in the treatment of cardiovascular diseases. We found that triterpenoid alkaloids extracted from Buxus sinica found in the Yunnan Province exhibit anticancer activity by depleting mutant p53 levels in colon cancer cells. Purpose To explore the anticancer mechanism of action of the triterpenoid alkaloid KBA01 compound by targeting mutant p53 degradation. Study design and methods Different mutant p53 cell lines were used to evaluate the anticancer activity of KBA01. MTT assay, colony formation assay and cell cycle analysis were performed to examine the effect of KBA01 on cancer cell proliferation. Western blotting and qPCR were used to investigate effects of depleting mutant p53, and a ubiquitination assay was used to determine mutant p53 ubiquitin levels after cells were treated with the compound. Co-IP and small interfering RNA assays were used to explore the effects of KBA01 on the interaction of Hsp90 with mutant p53. Results The triterpenoid alkaloid KBA01 can induce G2/M cell cycle arrest and the apoptosis of HT29 colon cancer cells. KBA01 decreases the stability of DNA contact mutant p53 proteins through the proteasomal pathway with minimal effects on p53 mutant protein conformation. Moreover, KBA01 enhances the interaction of mutant p53 with Hsp70, CHIP and MDM2, and knocking down CHIP and MDM2 stabilizes mutant p53 levels in KBA01-treated cells. In addition, KBA01 disrupts the HSF1-mutant p53-Hsp90 complex and releases mutant p53 to enable its MDM2- and CHIP-mediated degradation. Conclusion Our study reveals that KBA01 depletes mutant p53 protein in a chaperone-assisted ubiquitin/proteasome degradation pathway in cancer cells, providing insights into potential strategies to target mutant p53 tumours.

A coordination polymer [Zn(H-ntb)]n (1) based on the tritopic carboxylate linker of 4,4′,4′'-nitrilotribenzoate (H3-ntb) was reported here. Compound 1 crystallizes in a polar space group of Pca21 and contains two-dimensional layers formed from the left- and right-handed helical chains pillared by the benzoate moieties to give a three-dimensional structure. The present compound 1 features a noncentrosymmetric structure and displays a strong phase-matchable second-harmonic-generation (SHG) response of 1.2 times that of potassium dihydrogen phosphate (KDP). The inherent polarity of the 4,4′,4′'-nitrilotribenzoate and distortive [ZnO4] tetrahedrons as well as the asymmetric arrangement of the building blocks are responsible for the SHG activity for 1.

It is valuable to develop a sensing platform for not only detecting a tumor marker in body fluids but also measuring its expression at single cells. In the present study, a simple closed bipolar electrodes-based electrochemiluminescence (BPEs-ECL) imaging strategy was developed for visual immunoassay of prostate specific antigen (PSA) at single cells using functional nanoprobes of heterogeneous Ru(bpy)

Adjuvant tamoxifen reduces the recurrence rate of estrogen receptor (ER)-positive breast cancer. Previous in vitro studies have suggested that tamoxifen can affect the cancerous inhibitor of protein phosphatase 2A (CIP2A)/protein phosphatase 2A (PP2A)/phosphorylation Akt (pAkt) signaling in ER-negative breast cancer cells. In addition to CIP2A, SET nuclear proto-oncogene (SET) oncoprotein is another intrinsic inhibitor of PP2A, participating in cancer progression. In the current study, we explored the clinical significance of SET, CIP2A, PP2A, and Akt in patients with ER-positive breast cancer receiving adjuvant tamoxifen. A total of 218 primary breast cancer patients receiving adjuvant tamoxifen with a median follow-up of 106 months were analyzed, of which 17 (7.8%) experienced recurrence or metastasis. In an immunohistochemical (IHC) stain, SET overexpression was independently associated with worse recurrence-free survival (RFS) (hazard ratio = 3.72, 95% confidence interval 1.26–10.94, p = 0.017). In silico analysis revealed mRNA expressions of SET, PPP2CA, and AKT1 significantly correlated with worse RFS. In vitro, SET overexpression reduced tamoxifen-induced antitumor effects and drove luciferase activity in an Estrogen receptor element (ERE)-dependent manner. In conclusion, SET is a prognostic biomarker in patients with primary ER-positive breast cancer receiving adjuvant tamoxifen and may contribute to the failure of the tamoxifen treatment by modulating the ER signaling. Our study warrants further investigation into the potential role of SET in ER-positive breast cancer.

Aims Invasive breast cancer patients with human epidermal growth factor receptor 2 (HER2) immunohistochemical (IHC) scores of 3+ or 2+ with reflex in-situ hybridisation (ISH) positivity are suitable for anti-HER2 therapies. The aim of this study is to investigate whether the prognoses between IHC 3+ patients and IHC 2+/ISH+ patients are different. Methods and results We analysed the clinicopathological information of 886 consecutive cases of HER2-positive early breast cancer. The influences of the patients' age, cancer stage, hormone receptor status and anti-HER2 treatment were adjusted using a multivariate Cox regression model. Both HER2 copy numbers and HER2 ISH ratios of the IHC 3+ group were significantly higher than those of the IHC 2+/ISH+ group. The outcomes of IHC 3+ patients were significantly better than those of IHC 2+/ISH+ patients in the univariate and multivariate analyses. HER2 copy numbers of ≥8 represented the best prognostic value, and it was chosen to be the cut-off value. The reflex ISH for IHC 2+ patients with high HER2 copy numbers (≥8) predicted a better overall survival than that for those with low HER2 copy numbers. Conclusion HER2 IHC scores and HER2 copy numbers can provide prognostic information for patients with HER2-positive invasive breast cancer. Both IHC 3+ and IHC 2+ patients with high HER2 copy numbers had a better prognosis.

Background: This systematic review was aimed to evaluate the efficacy and safety of cupping therapy for treating patients with knee osteoarthritis (KOA). Methods: The following databases were searched from their inception until June 2017: the Cochrane Central Register of Controlled Trials, PubMed, EMBASE, and 4 Chinese databases (Wan Fang Data, Chinese Biomedical Literature Database (CBM), VeiPu, and China National Knowledge Infrastructure (CNKI)). Randomized controlled trials (RCTs) assessing the effects of cupping therapy on KOA were included in this systematic review. A quantitative synthesis of the RCTs was conducted using RevMan 5.3 software. Study selection and data extraction and validation were performed independently by 2 reviewers. Cochrane criteria for risk of bias were used to assess the methodological quality of the trials. Results: A total of 5 studies met our inclusion criteria. We analyzed the data from these 5 RCTs involving 535 participants. All included studies were judged to be at high risk for bias. Dry cupping therapy plus Western medicine therapy was more effective than Western therapy alone in reducing the pain scores (mean difference (MD) = -1.79, 95% confidence interval (CI) -2.40 to -1.18; p < 0.01). In addition, the study participants in the dry cupping therapy plus Western medicine therapy group showed significantly greater improvements in the pain (MD = -0.73, 95% CI -1.61 to -0.41; p < 0.01), stiffness (MD = -0.94, 95% CI -1.30 to -0.58; p < 0.01), and physical function (MD = -10.07, 95% CI -13.45 to -6.69; p < 0.01) domains of the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) compared to participants in the Western medicine therapy group. Moreover, when compared with Western medicine therapy alone, a meta-analysis of 4 RCTs suggested statistically significant favorable effects of wet cupping therapy plus Western medicine on the Lequesne Algofunctional Index (LAI) (MD = -3.44, 95% CI -4.21 to -2.68; p < 0.01). Conclusion: There is weak evidence to support the hypothesis that cupping therapy has beneficial effects on reducing the pain intensity and improving the physical function in patients with KOA.

The benzophenone-3,3′-disulfonyl-4,4′-dicarboxylic acid (H4–BODSDC) ligand and compounds, {(H3O)[Ln(BODSDC)(H2O)2]}n (Ln = Tb(1), Eu(2), and Gd(3)), were synthesized and structurally characterized. The lanthanide centers are bridged by the carboxylate groups of BODSDC4– ligands to give a one-dimensional (1D) chain. The 1D chains are connected by the BODSDC4– ligands to yield a three-dimensional (3D) structure featuring 1D channels. The lanthanide ions are efficiently sensitized by the BODSDC4– ligand with an appropriate triplet excited state to generate characteristic Tb(III) and Eu(III) emissions in Tb(1) and Eu(2), respectively. Thus the binary compound, {(H3O)[Tb0.93Eu0.07(BODSDC)(H2O)2]}n (abbreviated as Tb0.93Eu0.07-BODSDC), was achieved for use as a ratiometric temperature sensor. The ratio values of Tb(III) emission at 544 nm (ITb) and Eu(III) emission at 616 nm (IEu) for Tb0.93Eu0.07-BODSDC linearly vary with temperature over a wide range, which indicates that the Tb0.93Eu0.07-BODSDC is a thermome...

Total flavones ofRhododendron simsiiPlanch flower (TFR) have a significant protective effect against cerebral ischemia-reperfusion injury. However, its mechanism is unclear. This study investigated the protection of TFR against cerebral ischemia-reperfusion injury via cystathionine-γ-lyase- (CSE-) produced H2S mechanism. CSE-/-mice and CSE-siRNA-transfected rat were used. Relaxation of cerebral basilar artery (CBA), H2S, and CSE mRNA were measured. TFR significantly inhibited cerebral ischemia-reperfusion-induced abnormal neurological symptom and cerebral infarct in the normal rats and the CSE+/+mice, but not in the CSE-/-mice, and the inhibition was markedly attenuated in CSE-siRNA-transfected rat; TFR elicited a significant vasorelaxation in rat CBA, and the relaxation was markedly attenuated by removal of endothelium or CSE-siRNA transfection or coapplication of NO synthase inhibitor L-NAME and PGI2synthase inhibitor Indo. CSE inhibitor PPG drastically inhibited TFR-evoked vasodilatation resistant to L-NAME and Indo in endothelium-intact rat CBA. TFR significantly increased CSE mRNA expression in rat CBA endothelial cells and H2S production in rat endothelium-intact CBA. The increase of H2S production resistant to L-NAME and Indo was abolished by PPG. Our data indicate that TFR has a protective effect against the cerebral ischemia-reperfusion injury via CSE-produced H2S and endothelial NO and/or PGI2to relax the cerebral artery.

An efficient, environmentally benign, and inexpensive procedure has been developed for the synthesis of fluorinated 2-alkylthio-4-aminoquinazolines by microwave irradiation using basic alumina as a solid-support agent as well as a solid base. Notably, this protocol features improved energy efficiency, broad isothiourea substrate scope, easily available starting materials, and high atom efficiency and applicability toward gram-scale synthesis. Additionally, the target compounds were evaluated for the cytotoxic effect against human colon adenocarcinoma (HCT116 and HT29), human gastric cancer (SGC-7901), human lung adenocarcinoma (A549), and human hepatocyte carcinoma (HepG2) cells, and it was found that these compounds have excellent antitumor activities. Among them, compound 3e was found to be one of the most potent derivatives with IC50 values lower than 9.44 μM against five human tumor cell lines, making it more active than cisplatin (DDP). Furthermore, for the first time, the fluorinated 2-alkylthio-substituted 4-aminoquinazolines were identified as phosphatase CDC25B inhibitors.

Forest musk deer(Moschus berezovskii) is mainly distribute in Shaanxi and Sichuan provinces of China. The musk is a dry exudates in the mature male sachet with complex chemical composition, abundant modern identification methods, various pharmacological action, extensive clinical application. The population quantity and quality of wild and captive forest musk deer failed to improve because of lacking scientific management, excessive hunting and habitat destruction, etc. Carrying out the captive forest musk deer is an effective way to protect them, fast breeding of forest musk deer is the key to increase the yield of musk. Therefore, we should develop new technologies such as natural estrus and artificial insemination in order to speed up the breeding of musk deer and increase the yield of musk. This article reviews the research progress of new artificial propagation technique and musk, which teases some feasible research basis for optimization of forest musk deer species and the research of musk.

A new compound, {[Co(bmzbc)(2)]center dot 2DMF}(n) (JXNU-1, JXNU denotes Jiangxi Normal University), based on the 4-(benzimidazole-1-yl)benzoate (bmzbc(-)) ligand has been synthesized and structurally characterized. The Co(II) ions are bridged by the rod-like bmzbc(-) ligands to give a two-dimensional (2D) sheet wherein the Co(II) ions are spatially separated from each other by the long bmzbc- rods. The 2D sheets are further stacked into a 3D framework with ID channels occluding the guest DMF molecules. Detailed magnetic studies show that the individual octahedral Co(II) ions in JXNU-1 exhibit field-induced slow magnetic relaxation, which is characteristic behavior of single-ion magnets (SIMs). The rarely observed positive value of zero-field splitting (ZFS) parameter D for the Co(II) ion in JXNU-1 demonstrates that JXNU-1 is a unique example of Co(II)-based SIMs with easy-plane anisotropy, which is also confirmed by the calculations. The microporous nature of JXNU-1 was established by measuring CO2 sorption isotherms. The abrupt changes observed in the C3H8 and C2H6 adsorption isotherms indicate that a structural transformation occurred in the gas-loading process. The long connection between the magnetic metal centers in JXNU-1 meets the requirements for construction of porosity and SIM in a well-defined network, harmoniously providing a good candidate of functional molecular materials exhibiting SIM and porosity.

In order to exactly master effect of plutonium material’s heat power on safety and reliability of nuclear weapon, weapon-grade plutonium(WgPu) was regarded as research object in this paper. According to its component, decay heat power of WgPu per kilogram was calculated through dynamic decay cascade and decay law of plutonium. The maximum decay heat power of WgPu is 2.6158 watt per kilogram at seventy years.

With advances in biomedical methods, tissue-engineered materials have developed rapidly as an alternative to nerve autografts for the repair of peripheral nerve injuries. However, the materials selected for use in the repair of peripheral nerve injuries, in particular multiple injuries and large-gap defects, must be chosen carefully. Various methods and materials for protecting the healthy tissue and repairing peripheral nerve injuries have been described, and each method or material has advantages and disadvantages. Recently, a large amount of research has been focused on tissue-engineered materials for the repair of peripheral nerve injuries. Using the keywords "pe-ripheral nerve injury", "autotransplant", "nerve graft", and "biomaterial", we retrieved publications using tissue-engineered materials for the repair of peripheral nerve injuries appearing in the Web of Science from 2010 to 2014. The country with the most total publications was the USA. The institutions that were the most productive in this field include Hannover Medical School (Germany), Washington University (USA), and Nantong University (China). The total number of publications using tissue-engineered materials for the repair of peripheral nerve injuries grad-ually increased over time, as did the number of Chinese publications, suggesting that China has made many scientific contributions to this field of research.

Many flavonoids have cardioprotection against myocardial ischemia/reperfusion (I/R) injury. Total flavones fromRhododendron simsiiPlanch flower (TFR) can protect myocardial ischemic injuries. However, its protective mechanism is still unknown. The present study was designed to investigate the mechanism of TFR on myocardial I/R and anoxia/reoxygenation (A/R) injuries. Rat model of myocardial I/R injury was made, and myocardial infarction was determined. A/R injury was induced in cultured rat cardiomyocytes; cellular damage was evaluated by measuring cell viability, LDH and cTnT releases, and MDA content. Expressions of ROCK1and ROCK2protein were examined by Western blot analysis, and K+currents were recorded by using whole-cell patch clamp technique. TFR 20~80 mg/kg markedly reduced I/R-induced myocardial infarction. TFR 3.7~300 mg/L significantly inhibited A/R-induced reduction of cell viability, LDH and cTnT releases, and MDA production. Exposure to A/R significantly increased ROCK1and ROCK2expressions in rat cardiomyocytes, but TFR 33.3~300 mg/L obviously inhibited this increase. 300 mg/L TFR significantly augmented inward rectifier K+current and other K+currents in rat cardiomyocytes. These results indicate that TFR has a protective effect on rat cardiomyocytes A/R damage, and the protective mechanism may be engaged with the inhibition of ROCK1and ROCK2and activation of K+channels.

2-Fold interpenetrating chiral quartz nets with opposite handedness have been observed in a centrosymmetric coordination polymer {[Zn3(bmzbc)6]·11.5DMF}n (Hbmzbc = 4-(benzimidazole-1-yl)benzoic acid), which is a unique example of an interpenetrating enantiomorphic pair of quartz nets that have never been realized before.

The highly odd-numbered 15-connected nonanuclear [Ln9(μ3-O)2(μ3-OH)12(O2C−)12(HCO2)3] and 9-connected trinuclear [Ln3(μ3-O)(O2C−)6(HCO2)3] lanthanide-carboxylate clusters with triangular and linear carboxylate bridging ligands were synergistically combined into Ln-MOFs, [(CH3)2NH2]3{[Ln9(μ3-O)2(μ3-OH)12(H2O)6][Ln3(μ3-O)(H2O)3](HCO2)3(BTB)6}·(solvent)x (abbreviated as JXNU-3, Ln = Gd, Tb, Er; BTB3– = benzene-1,3,5-tris(4-benzoate)), displaying a (3,9,15)-connected topological net. The JXNU-3(Tb) exhibits highly selective CO2 adsorption capacity over CH4 that resulted from the high localized charge density induced by the presence of the nonanuclear and trinuclear cluster units. In addition, JXNU-3(Tb) with high chemical stability and characteristic bright green color exhibits fluorescent pH sensing, which is pertinent to the different protonation levels of the carboxylate groups of the benzene-1,3,5-tris(4-benzoate) ligand with varying pH.

A dysprosium-carboxylate coordination polymer, {[Dy2(2,2′-bpdc)3(e-urea)(H2O)](e-urea)}n (1) (2,2′-bpdc2 − = 2,2′-biphenyldicarboxylate and e-urea = ethyleneurea), has been synthesized using a deep-eutectic solvent of choline chloride/e-urea. Four Dy3 + ions are linked by the carboxylate oxygen atoms of the 2,2′-bpdc2 − ligands to form a linear Dy4 unit, which is further linked by the carboxylate groups of the 2,2′-bpdc2 − ligands to give a rare example of one-dimensional chain based on the linear Dy4 secondary building units. Detailed static and dynamic magnetic analysis of 1 revealed that 1 exhibits a slow magnetic relaxation behavior.

A photovoltaic cell company located in China used with double pass reverse osmosis method to treat low concentration fluoride wastewater. Product water entered the pure water preparation system to recycle. The design capacity of wastewater was 180m3/h. The project improved the pretreatment methods, the operating conditions and the cleaning methods of membrane, so as to prevent membrane blocked and increase of service life. This project ran stability.The water treatment cost was about 4 yuan/t, lower than the local water price.

In this paper, the problem of adaptive synchronization in pth moment is considered for stochastic complex networks with time varying delayed and no-delayed couping. By using the Lyapunov Krasovskii functional, stochastic analysis theory, sufficient condition to ensure adaptive synchronization in pth moment for stochastic time varying delayed complex networks is derived. To illustrate the effectiveness of the synchronization condition derived in this paper, a numerical example is provided finally.

Two cadmium(II) coordination polymers, namely, [Cd-3(m-phth)(2)(atz)(2)](n) (1) (m-phth = m-phthalate and atz = 3-amino-1,2,4-triazolate) and [Cd(atphth)(H2O)](n) (2) (atphth = 2-aminoterephthalate), were synthesized and structurally characterized. Compound 1 features a three-dimensional (3D) pillared framework based on two-dimensional (2D) cadmium-benzenedicarboxylate ladders pillared by the triazolate ligands. Compound 2 has a 3D framework constructed from 2D cadmium-benzenedicarboxylate layers, which are further linked by Cd-N bonds between the cadmium ions and amino groups of the atphth(2-) ligands of the adjacent layers to form the final 3D structure. Compounds 1 and 2 exhibit solid-state photoluminescence with emission maxima at 448 and 470 nm, respectively.

Bollobas and Gyarfas conjectured that for n > 4 (k − 1) every 2-edge-coloring of K n contains a monochromatic k-connected subgraph with at least n − 2k + 2 vertices. Liu, et al. proved that the conjecture holds when n ≥ 13k − 15. In this note, we characterize all the 2-edge-colorings of K n where each monochromatic k-connected subgraph has at most n−2k+2 vertices for n ≥ 13k − 15.

In order to exactly master effect of uranium material’s heat power on safety and reliability of nuclear weapon, weapon-grade uranium(WgU) and depleted uranium(DU) were regarded as research object in this paper. According to their component, decay heat power of WgU and DU per kilogram was calculated through dynamic decay cascade and decay law of uranium. Decay heat power of WgU and DU was respectively 1.8152 and 0.4075 milliwatt per kilogram. Main nuclide influencing on decay heat power of WgU and DU is uranium-234(U-234).

Two new cadmium(II) coordination compounds, [Cd4(SBTC)2(2,2′-bpy)6(H2O)4](2,2′-bpy) (1) (2,2′-bpy = 2,2′-bipyridyl) and {[K2Cd(SBTC)(H2O)4](H2O)2}n (2), were synthesized using a novel ligand of 5-sulfonyl-1,2,4-bezenetricarboxylic acid (H4-SBTC) featuring three carboxylate groups and one sulfonate group stationed on a phenyl central motif. Compound 1 is a discrete tetranuclear [Cd4(SBTC)2(2,2′-bpy)6(H2O)4] structure with a solvated 2,2′-bpy molecule, wherein the four cadmium ions are bridged by two μ3-SBTC4– ligands. The discrete tetranuclear [Cd4(SBTC)2(2,2′-bpy)6(H2O)4] motif is extended into a three-dimensional (3D) supramolecular framework through hydrogen bonds and π–π interactions. In compound 2 the cadmium ions and potassium ions are interconnected by the SBTC4– ligands and bridging water molecules to give a complex 3D structure. The 3D structure is constructed by a rare 3D inorganic cadmium-potassium-oxide framework, in which the cadmium and potassium ions are bridged by the sulfonate groups and bridging water molecules. In the solid state, compounds 1 and 2 exhibit photoluminescence with emission maximum at 380 and 445 nm, respectively.

Three-dimensional electrode method is especially suitable for the treatment of low concentration wastewater containing Cu2+. Study the changes on the Cu2+ removal rate, under the condition of different electrode filling material (such as steel ball, activated carbon, activated carbon and resin). The results show steel ball as filling-material is best. Steel ball has higher removal efficiency of Cu2+, up to more than 80%. Steel ball as filling-material the effect of electrode distance on Cu2+ removal is smallest. Compared with steel ball as filling-material, the response time of activated charcoal is no obvious disadvantage.

Two mononuclear compounds Ln(e-urea)8(NO3)3 (Ln = Dy(1) and Tb(2)) have been urothermally synthesized by using the ethyleneurea (e-urea) as an organic ligand and a solvent. The e-urea ligand with a single donor atom is a unique ligand for coordinating to lanthanide ions, which is very different from those commonly observed polytopic ligands with suitable coordination pockets to encapsulate lanthanide(III) ions or chelating organic ligands. The lanthanide center surrounded by eight e-urea ligands is in a square antiprismatic environment with an approximate D4d local symmetry. Magnetic properties of both compounds have been investigated in detail using direct-current and alternating-current susceptibility measurements. The Dy(1) analogue displays slow magnetic relaxation behavior with an effective energy barrier of the magnetic relaxation of 37.68 K and the pre-exponential factor τ0 of 1.47 × 10−9 s in the presence of direct-current field of 2 kOe.

Objective: To observe the clinical effect of warm moxibustion therapy and acupoint application therapy on recovery of gastrointestinal functions in postoperative patients with colorectal cancer. Method: Eighty patients with colorectal cancer were admitted to Department of Gastrointestinal surgery Tianjin Nankai Hospital. A retrospective analysis of 20 cases as control group. 60 patients near admitted and the use of randomly divided into three treatment groups, 20 cases of each group. The control group received conventional therapy and nursing, and three treatment groups respectively received warm moxibustion therapy, acupoint application therapy and both the two methods above together additionally. Then compared the intervention effect of them. Results: General information on four groups of comparison, there was no statistics significance (P > 0.05), between the four groups were comparable. Analysis of variance of repeated measures indicated that the interaction effect of intervention time and grouping had significant influence on Chinese medicine symptom. Two groups of the Chinese medicine symptom integral changing are inconsistent (P < 0.01). Chinese medicine symptom efficacy total effective rate of treatment group was superior to the control group (P < 0.01). And first postoperative exhaust time, defecating time and eating time were earlier than control group (P < 0.01). Conclusion: Warm moxibustion therapy and acupoint application therapy had certain clinical effect on the recovery of gastrointestinal function in patients with colorectal cancer after operation, and worthy of further application in clinical practice.

Objective: To explore and analyze the effect of moxibustion combined with acupoint application on enteral nutrition tolerance, nutritional status and comfort in patients with severe acute pancreatitis. Methods: Ninety-six patients who were hospitalized in a third-grade class-A general hospital in Tianjin were selected and divided into three groups: A, B, and C, each with 32 cases. Group A uses usual treatment and care methods, group B implements acupoint application treatment, group C was treated with moxibustion on the basis of acupoint application. Intervention time is from the first day of the enteral nutrition to the 7th day, the tolerance of enteral nutrition in patients 7 days during the intervention and the level of serum albumin and pre-protein in the nutritional indicators of patients on day 3 of intervention and inter-vention on day 7, as well as the comfort level of the patients on the 7th day of intervention were observed and recorded. Results: (1) The difference in the incidence of enteral nutrition intolerance among the three groups was significantly statistical (P < 0.05). The incidence of enteral nutrition intolerance in group C was lower than that in group B (P < 0.05) and group A (P < 0.05). There was no significant dif-ference between group B and group C (P > 0.05). (2) Nutritional index levels of three groups of patients, on the third day of intervention, the serum albumin and serum pre-protein were significantly statistical (P < 0.05). Comparing the two groups, the serum albumin level in group A was significantly different from that in group B (P < 0.05). There was significant difference between group A and group C (P < 0.05). There was no significant difference between the group and the group C (P > 0.05). On the 7th day of intervention, the difference between group A and group B was statistically significant (P < 0.05). There was a statistically significant difference between group A and group C (P < 0.05), group B and group C (P < 0.05). Pre-protein levels in three groups, there was a significantly statistical difference be-tween intervention days 3 and 7 (P < 0.05), further comparison was made between the two groups. On the third day of intervention, there were significant differences between group A and group B, group A and group C, group B and group C (P < 0.05). On the 7th day of in-tervention, there were significant differences between group A and group B, group A and group C, group B and group C (P < 0.05). (3) On the 7th day of intervention, the comfort level of the three groups was significantly different (P < 0.05). Further comparison between the two groups was made. The comfort score of group C was higher than that of group B and group A (P < 0.05), and the score of group B was higher than group A (P < 0.05). Conclusion: Moxibustion combined with acupoint application can effectively improve the tolerance level of enteral nutrition and improve the nutritional status of patients and increase patient comfort.

To study the effects and optimal operating parameters of three-dimensional electrode electrolysis process for PCB wastewater containing Cu2+, the effects of electrolytic voltage, plate distance, material of plate, filling element and electrolyte were inspected to decide optimal experiment conditions. The experimental result showed that the optimal conditions were 3.0cm plate distance, steel ball as the filling material, 10V electrolytic voltage, and 45 min electrolytic time, and the removal rate of Cu2+ wastewater reaches 82.3%. The electricity costs were 1.11 yuan/m3.

A solution based flexible N-type organic TFTs (OTFTs) backplane on polymer film is developed and processed in an existing Gen-2 a- Si TFT production line. The integrated OTFTs device shows good mobility at 1.5 cm2/Vs, Vth at 2V and SS smaller than 1. A Flexible Electrophoretic Display (EPD) module driven by this N-type OTFTs backplane is also developed. The optical performance of this flexible EPD module remains unchanged even after bending operation, which enables the advent of a truly flexible display application in the near future.

2-Hydroxy-4-methoxy acetophenone condensed the Aminobenzenearsonic Acid was synthesized by paeonol and aminobenzenearsonic acid. that is, arsanilic compounds (3b). At the same time, the compound was characterized by IR-NMR-MS, as well as four methyl thiazolyl tetrazolium (MTT) colorimetric assay the arsine compounds (3b) on growth inhibition of human hepatoma cell lines HepG2 cell at different times under different concentration. It is shown that the arsine compounds (3b) can significantly inhibit the growth of human hepatoma cell line HepG2. Its effects were dose-and time-dependent. It is indicated a potential anti-tumor activity.

Analyze the safety of the wire rope fixtures through experiment while constructing the COMAC ministry of installed plant. Pointed out the proper use of the provisions of the steel erection specification, and the number of provisions chuck is unsafe. The timely replacement of the shackle wire rope connection ensured the security of the lift process through experiments. This method can provide some experiences to the construction and fabrication of the steel member of the project.

The flexible overall hoisting technique is put forward for the construction of the 30000m2 COMAC roof which is made of solder ball grid structure. The numerical simulation is carried out and the effect of unequal lifting on structural behaviors is analyzed. Numerical analysis and engineering practicing show that the overall flexible hoisting technique is with sufficient safety and obvious advantage in economy.

Two coordination compounds with novel sulfonate–carboxylate ligand of 2,5-disulfonylterephthalate acid (H 4 DSBDC), namely, [Ni(2,2′-bpy)(H 2 O) 4 ](DSBDC) 0.5 ( 1 ) and [Mn(DSBDC) 0.5 (2,2′-bpy)(H 2 O) 2 ] n ( 2 ) (2,2′-bpy = 2,2′-bipyridyl), have been synthesized. In situ formation of H 4 DSBDC ligand derived from 5-sulfonyl-1,2,4-benzenetricarboxylic acid is unprecedented and involves a fundamentally new substitution of sulfonate for carboxylate to in situ ligand synthesis reaction. Compound 1 has a mononuclear nickel [Ni(2,2′-bpy)(H 2 O) 4 ] 2+ motif charged with tetraanionic DSBDC 4− ion. The [Ni(2,2′-bpy)(H 2 O) 4 ] cations and DSBDC 4− anions are linked by extensive hydrogen bonds to form a three-dimensional supramolecular structure. Compound 2 is a one-dimensional polymeric chain based on the dinuclear Mn 2 units. The one-dimensional chains are linked by hydrogen bonds and π–π interactions to form a three-dimensional supramolecular structure. The temperature-dependent magnetic susceptibility data of 1 and 2 have been investigated. The Curie constant C and Weiss constant θ are 1.29 cm 3 K mol −1 and −1.61 K for compound 1 , respectively, revealing antiferromagnetic interactions between the Ni(II) ions. The magnetic data of 2 are fitted with the dinuclear Mn 2 model giving the best fitting results of g = 2.04, J = −0.68, and zj ′ = −0.016 cm −1 , which indicate the antiferromagnetic interactions are present in 2 .

[Yb2(SBTC)(ox)(H2O)5] n (1) (H4-SBTC = 5-sulfonyl-1,2,4-benzenetricarboxylic acid and ox = oxalate) has been hydrothermally synthesized by reaction of 5-sulfonyl-1,2,4-benzenetricarboxylic acid with Yb(NO3)3∙5H2O. In situ formation of oxalate derived from H4-SBTC is unprecedented and adds a new oxalate formation reaction to in situ ligand syntheses. Compound 1 features a 2-D bilayered coordination network, which is further extended into a 3-D supramolecular framework through interlayered hydrogen bonds. Magnetic measurements on 1 indicate that there is an antiferromagnetic interaction between Yb(III) ions.

There are six key points, such as steel truss column, hoisting frame, hoisting point, wire rope, wire rope connecting in the hoisting system which we construct the COMAC ministry of installed plant. This timely monitoring of several key points in the promotion process is to enhance the process controlled several indicators. For different key points used different monitoring methods for monitoring. The results showed that 6 monitoring method is reasonable, feasible. This method can provide some experiences to the construction and fabrication of the steel member of the project.

The flexible overall hoisting technique is put forward for the construction of the 30000m2 COMAC roof which is made of solder ball grid structure. It is critical to study how to properly measure the four ropes length of each lifting point. Language tools use in AUTOCAD AUTOLISP appropriate programming has been an efficient tool to correctly output the length of the rope. This method can provide some experiences to the construction and fabrication of the steel member of the project.

Background: Airway granulation tissue and scar formation pose a challenge because of the high incidence of recurrence after treatment. As an emerging treatment modality, topical application of mitomycin C has potential value in delaying the recurrence of airway obstruction. Several animal and clinical studies have already proven its feasibility and efficacy. However, the ideal dosage has still not been determined. Objectives: To establish a novel method for culturing primary fibroblasts isolated from human airway granulation tissue, and to investigate the dose-effect of mitomycin C on the fibroblast proliferation in vitro, so as to provide an experimental reference for clinical practitioners. Methods: Granulation tissues were collected during the routine bronchoscopy at our department. The primary fibroblasts were obtained by culturing the explanted tissues. The cells were treated with different concentrations of mitomycin C (0.1, 0.2, 0.4, 0.8 and 1.6 mg/ml) for 5 min followed by additional 48-hour culture before an MTT assay was performed to measure cell viability. Results: MTT assay showed that mitomycin C reduced cell viability at all tested concentrations. The inhibitory ratios were 10.26, 26.77, 32.88, 64.91 and 80.45% for cells treated with mitomycin C at 0.1, 0.2, 0.4, 0.8 and 1.6 mg/ml, respectively. Conclusions: Explant culture is a reliable method for culturing primary fibroblasts from human airway granulation tissue, and mitomycin C can inhibit proliferation of the fibroblasts in vitro.

In this paper, the initial residual stress field for titanium alloy monolithic component blank is simulated and analyzed by applying a sequentially coupled thermal and mechanical procedure based on finite element method. The control equation of heat conduction of annealing process is studied, and the boundary condition and fundamental equations of simulation are given. The research results show that the initial residual stress field for titanium alloy monolithic component blank radiates from the center region to outside, the center region being the maximum value, then decreases gradually, until a minimum value is attained at the corner.

MAPK signaling pathways are crucial in regulating osteogenesis, a genetic disorder affecting the bones. Quercitrin, a type of flavonoid, is widely distributed in nature and involved in many pharmacological activities. But its osteoprotective functions and mechanism in osteoporosis are far from being understood clearly. In this paper, the MAPK upregulation was observed in the ovariectomy-induced bone loss. Quercitrin was found to downregulate MAPK signaling pathways and prevent the ovariectomy-induced deterioration of bone mineral density (BMD), trabecular microstructure, and bone mechanical characteristics. In this study, quercitrin was seen to prevent the progression of the postmenopausal osteoporosis among the rats, which may be mediated by the downregulated MAPK signaling pathways.

Excessive inflammation contributes greatly to the pathogenesis and development of rheumatoid arthritis (RA). Oroxylin A (OA) is a natural anti-inflammatory flavonoid compound. In this study, we investigated the effects of OA on collagen-induced arthritis (CIA) and human RA fibroblast-like synoviocytes (FLS). CIA was induced in DBA/1 mice and mice were intraperitoneally treated with OA (10mg/kg) for 10days. Arthritis severity was evaluated every day and the histopathologic examination of joints was done. Serum levels of anti-collagen II antibodies (anti-CII Abs) and cytokines were determined by ELISA. Frequency of regulatory T cells (Tregs) and Th17 cells in draining inguinal lymph nodes (ILN) was quantified by flow cytometry. FLS from patients with active RA were treated with varying doses of oroxylin A, followed by stimulation with tumor necrosis factor (TNF)-α (10ng/mL). The production of cytokines was measured by ELISA. Signal transduction proteins were examined by western blot. OA significantly diminished the arthritis and histological damage. Serum anti-CII Abs, IL-1β, IL-6, TNFα, and IL-17 were significantly diminished by OA treatment. Analysis of CD4+T cell populations in OA-treated mice showed an increase in Tregs and reduction in Th17 cells in the ILN. In vitro, OA decreased the secretion of IL-1β and IL-6 from TNFα-stimulated RA FLS in a dose-dependent manner. TNFα-induced p38 MAPK, ERK1/2 and NF-κB signaling pathways were suppressed by OA. Our results indicate that OA exerts an anti-inflammatory activity and may have therapeutic potential for human RA.

Adhesion molecules expressed on cerebral endothelial cells (ECs) mediate leukocyte recruitment and play a significant role in cerebral inflammation. Increased levels of adhesion molecules on the EC surface induce leukocyte infiltration into inflammatory areas and are thus hallmarkers of inflammation. Honokiol, isolated from the Chinese medicinal herb Magnolia officinalis, has various pharmacological activities, including anti-inflammatory effects, yet the nature of honokiol targeting molecules remains to be revealed. Here, we investigated the inhibitory effect of honokiol on neutrophil adhesion and vascular cell adhesion molecule-1 (VCAM-1) expression, which underlie its molecular target and mechanisms for inactivating nuclear factor κ enhancer binding protein (NF-κB) in mouse cerebral ECs. Honokiol inhibited tumour necrosis factor-α (TNF-α)-induced neutrophil adhesion and VCAM-1 gene expression in cerebral ECs. The inflammatory transcription factor NF-κB was downregulated by honokiol. Honokiol significantly blocked TNF-α–induced NF-κB p65 nuclear translocation and degradation of the proteasome-dependent inhibitor of NF-κB α (IκBα). From docking model prediction, honokiol directly targeted the ubiquitin–ubiquitin interface of Lys48-linked polychains. Moreover, honokiol prevented the TNF-α-induced Lys48-linked polyubiquitination, including IκBα-polyubiquitin interaction. Honokiol has protective anti-inflammatory effects on TNF-α-induced neutrophil adhesion and VCAM-1 gene expression in cerebral ECs, at least in part by directly inhibiting ubiquitination-mediated IκBα degradation and then preventing NF-κB nuclear translocation.

Organizing magnetically isolated 3d transition metal ions, which behave as single-ion magnet (SIM) units, in a coordination network is a promising approach to design novel single-molecule magnets (SMMs). Herein 3D chiral and 2D achiral cobalt(ii) coordination compounds based on single metal nodes with a 4-(benzimidazole-1-yl)benzoic acid (Hbmzbc) ligand, namely, [Co(bmzbc)2(1,2-etdio)]n () (1,2-etdio = 1,2-ethanediol) and [Co(bmzbc)2(Hbmzbc)]n (), have been synthesized and structurally characterized. The 3D chiral structure with 2-fold interpenetrating qtz topological nets consisting of totally achiral components was obtained via spontaneous resolution, while the achiral structure is a 2D (4,4) net. In both structures, individual cobalt(ii) ions are spatially well separated by the long organic ligands in the well-defined networks. Magnetic measurements on and showed field-induced slow magnetic relaxation resulting from single-ion anisotropy of the individual Co(ii) ions. Analysis of the dynamic ac susceptibilities with the Arrhenius law afforded an anisotropy energy barrier of 16.8(3) and 31.3(2) K under a 2 kOe static magnetic field for and , respectively. The distinct coordination environments of the Co(ii) ions in and lead to the different anisotropic energy barriers.

Two cadmium(II) coordination compounds, [Cd3(CH3CO2)4(ad)2(CH3CN)2]n (1) and [Cd3(5-SIP)2(H-ad)2(H2O)6]n (2) (H-ad = adenine and 5-SIP = 5-sulfoisophthalate), were synthesized and characterized. Compound 1 features a two-dimensional (2-D) layered structure based on linear trinuclear [Cd3(CH3CO2)4] units bridged by monoanionic adenine ligands. In 2, the 5-SIP3− ligands link Cd(II) ions to form a one-dimensional (1-D) ladder, which is further linked by neutral adenine ligands to give a 2-D layered structure. In both structures, the carboxylate ligands link Cd(II) ions to form low-dimensional structures, which are further connected by adenine ligands to give high-dimensional structures. Compounds 1 and 2 exhibit emissions centered at 382 and 416 nm, respectively, which can be attributed to the ligand centered π–π* transition.