Search

In Northeast China's black soil rice cropping area, nitrogen (N) application is lower than in the south, yet excessive N fertilizer use persists, particularly in base fertilizers. This study aimed to assess the impact of reduced N and increased planting density on rice yields and photosynthetic matter production. From 2019 to 2020, a field split-plot experiment was conducted with two N rates (conventional N, CN: 120 kg ha−1 and reduced basal N, RN: 108 kg ha−1) and two planting densities (D1: 33.3 × 104 hills ha−1 and D2: 27.8 × 104 hills ha−1) using the rice varieties Kongyu131 (KY131) and Kendao24 (KD24). The results showed that RN increased the effective panicle formation rate but decreased the tiller numbers, dry matter accumulation, stems/sheaths transport capacity, leaf photosynthetic capacity, and yield by 2.67% compared to CN. D1 significantly boosted the dry matter accumulation, stems/sheaths transport, effective panicles, grains per square meter, and yield by 8.26% compared to D2. Interaction analysis revealed that RN under D2 conditions reduced the effective panicle percentage, harvest index, filled grain number, leaf area index (LAI), crop growth rate (CGR), and net assimilation rate (NAR) but increased the seed setting rate and 1000-grain weight. Under D1, RN reduced the LAI, CGR, and NAR at the tillering and heading stages but increased the NAR post-heading. Compared to CND2, RND1 increased the biomass, stems/sheaths transport, LAI, CGR, NAR, seed setting rate, 1000-grain weight, panicle numbers, and filled grains per square meter, compensating for the lower harvest index and effective panicle rate, achieving a 5.36% yield increase. KD24 outperformed KY131 in yield improvement. In summary, using 108 kg ha−1 with a planting density of 33.3 × 104 hills ha−1 promotes tillering, enhances photosynthetic substance production stems/sheaths dry matter transport, and increases rice yields. [ABSTRACT FROM AUTHOR]

In this study, we sequenced the complete mitochondrial genome of the shield-faced leaf-nosed bat (Hipposideros lylei Thomas, 1914) using the Illumina platform. The mitochondrial genome of H. lylei is 16,856 bp in length, encoding 37 genes, which include 13 protein-coding genes, 22 tRNA genes, two rRNA genes, one replication start, and one non-coding control region (D-loop) of 417 bp in length. It has a G + C content of 42.0%, lower than the A + T content, indicating an obvious AT base preference. Phylogenetic analyses revealed that H. lylei clusters with three species of the genus Hipposideros in one branch and is relatively closely related to H. armiger and H. larvatus. [ABSTRACT FROM AUTHOR]

The taste of umami peptide H-Lys-Gly-Asp-Glu-Glu-Ser-Leu-Ala-OH (LGAGGSLA) is controversial. One possible reason for this controversy is the use of chemically synthesized LGAGGSLA to confirm its taste. To explore other ways to further confirm the flavor of LGAGGSLA, we developed a new strategy to prepare a bio-source peptide by adopting a gene engineering method to express LGAGGSLA in recombinant Escherichia coli. In our previous work, we structured the LGAGGSLA recombinant expression system and optimized the culturing conditions for preparing a fusion protein. However, the fusion protein was not cleaved by enterokinase to obtain LGAGGSLA. Because the cleavage conditions of commercial enterokinase were not specific and recombinant engineered bacteria had the potential to be used in industrial processes, in this addendum, we calculated the mass and volume yields of key processing steps in the preparation of LGAGGSLA, and established a model of cleavage conditions with the cleavage ratio of LGAGGSLA. When the LGAGGSLA was confirmed to show umami taste, it is considered as a new umami or umami enhancer. The gene information of LGAGGSLA should have a great potential in the development of new flavor product and food product containing high umami flavor.

Background: the feasibility of the capacitance method for detecting the water content in standing tree trunks was investigated using capacitance-based equipment that was designed for measuring the water content of standing tree trunks. Methods: In laboratory experiments, the best insertion depth of the probe for standing wood was determined by measurement experiments conducted at various depths. The bark was to be peeled when specimens and standing wood were being measured. The actual water content of the test object was obtained by specimens being weighed and the standing wood being weighed after the wood core was extracted. Results: A forecast of the moisture content of standing wood within a range of 0 to 180% was achieved by the measuring instrument. The feasibility of the device for basswood and fir trees is preliminarily studied. When compared to the drying method, the average error of the test results was found to be less than 8%, with basswood at 7.75%, and fir at 7.35%. Conclusions: It was concluded that the measuring instrument has a wide measuring range and is suitable for measuring wood with low moisture content, as well as standing timber with high moisture content. The measuring instrument, being small in size, easy to carry, and capable of switching modes, is considered to have a good application prospect in the field of forest precision monitoring and quality improvement. [ABSTRACT FROM AUTHOR]

In order to explore the characteristics of the mitochondrial genome sequence of Pratt's leaf-nosed bat (Hipposideros pratti Thomas 1891) and understand their phylogenetic status in Chiroptera, this study determined the mitochondrial genome sequences of H. pratti from five regions in China using high-throughput sequencing technology, sequence assembly, and genome annotation. The results showed that these sequences contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 non-coding region, all exhibiting a significant AT bias. Based on the phylogenetic tree constructed using 13 protein-coding genes from 15 Chiroptera species, the study found that H. pratti from the five regions clustered together, and then clustered with H. lylei into a single clade. Meanwhile, H. pratti from Jiangxi, Fujian, and Guangdong regions of China showed closer genetic relationships, while H. pratti from Yunnan and Henan regions of China exhibited closer genetic relationships. This study not only supplemented the mitochondrial genome database of H. pratti but also laid a foundation for genetic variation, molecular classification, and evolutionary studies of H. pratti. [ABSTRACT FROM AUTHOR]

The seismic attributes of water‐rich sandstone contain much information about the rock's physical properties and seismic wave parameters. They are commonly used to predict the rock's physical properties (e.g. porosity). However, the seismic attributes of water‐rich sandstone are affected by porosity, water saturation and thickness. To eliminate the influence of thickness on the porosity prediction of water‐rich sandstone and improve the accuracy of the porosity prediction, the authors propose a Lambert W–R transform method to isolate the contribution of thickness and porosity from seismic attributes. First, a rock physical model is used to calculate the equivalent elastic parameters of water‐rich sandstones with different porosity values and water saturation levels. Second, the seismic attribute dataset of water‐rich sandstone is established by forward modelling the seismic response of the wedge‐shaped water‐rich sandstone model, and the selection of sensitive physical properties is completed. Then, the transformation parameters (ζAhRs and ηAhRs) are obtained by Lambert W–R transformation, which is exponentially related to instantaneous amplitude. ζAhRs and ηAhRs are sensitive to thickness and porosity, respectively. Finally, an interpretative template for porosity prediction of water‐rich sandstone is established by cross‐plot analysis (ζAhRs and ηAhRs) and verified by a practical case. The verification results show that the porosity predicted by the interpretation template is consistent with drilling fluid consumption. However, it is lower than the porosity of logging constrained P‐wave impedance inversion. [ABSTRACT FROM AUTHOR]

Diabetic nephropathy (DN) is a primary diabetic complication ascribed to the pathological changes in renal microvessels. This study investigated the nuclear factor erythroid 2‐related factor 2 (Nrf2)/Kelch ECH associating protein (Keap1)/antioxidant response element (ARE) signaling pathway impact of chitooligosaccharides (COS) with a certain degree of polymerization (DP) on DN mouse models and high glucose‐damaged human kidney 2 (HK‐2) cells. The findings indicated that COS effectively reduced the renal function indexes (uric acid [UA], urinary albumin excretion rate [UAER], urine albumin‐to‐creatinine ratio [UACR], blood urea nitrogen [BUN], and creatinine [Cre]) of DN mice. It increased (p <.05) the superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), and catalase (CAT) antioxidant enzyme activity in the serum and kidneys, and decreased (p <.05) the malondialdehyde (MDA) content. The mechanistic investigation showed that COS significantly increased (p <.05) Nrf2 and downstream target gene (GCLM, GCLC, HO‐1, and NQO‐1) expression, and substantially decreased (p <.05) Keap1 expression. The protein level was consistent with the messenger RNA (mRNA) level in in vitro and in vivo models. The docking data indicated that COS and Keap1 protein binding included six hydrogen bond formation processes (Gly364, Arg415, Arg483, His436, Ser431, and Arg380). The COS intervention mechanism may be related to the Nrf2/Keap1/ARE antioxidant pathway. Therefore, it provides a scientific basis for COS application in developing special medical food for DN patients. [ABSTRACT FROM AUTHOR]