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Waterborne polyurethane (WPU) dispersions were prepared by post-chain extension and crosslinking reactions using 2-[(2-aminoethyl)-amino]ethyl sulfonic acid sodium (AAS), diethylenetriamine (DETA), triethylenetetramine (TETA) and AAS/DETA as post-chain extenders, and their influence on the properties of WPUs was studied. Results showed the introduction of trifunctional DETA chain extenders significantly improved the molecular weight, T-peel strength, and water resistance of WPUs, suggesting that DETA was an effective crosslinking agent. The WPU dispersions with a 2 : 3 molar ratio of AAS/DETA chain extenders exhibited excellent properties. At a molar ratio of 1.6 for NCO groups to OH groups, a stable WPU dispersion possessed higher solid content of 42.8%. Furthermore, only 4 wt% organic solvents were used in the preparation of WPU dispersions. Results revealed that the obtained WPU dispersions are a potential candidate for binders in water-based inks, without removing organic solvents. This study may provide some insight or guidance for the design and preparation of water-based ink binders.

Sulfidation of zero-valent iron (SZVI) emerged as a promising approach to enhance both the reactivity and selectivity of nano zero-valent iron (ZVI).

In recent years, the demand for cytopathological accurate diagnoses has increased as expanding minimally invasive procedures obtain materials from patients with advanced cancer for diagnostic, prognostic, and predictive purposes. However, inadequate knowledge of cytopathological technical procedures and ancillary techniques by clinicians remains the most common reason for the limited availability of cytopathology. The objectives of this review were to understand the technical procedures, ancillary techniques, and application and effectiveness of various types of tests in cytopathology. Each of the many ancillary technologies described in the literature has specific advantages and limitations and laboratories select one or more methods depending on their infrastructure and expertise to achieve the goal from initial screening of the disease to the final diagnosis of the cytopathology. This paper systematically reviews the development of cytopathology, summarizes the existing problems in cytopathology and the new progress of auxiliary examination, to provide a theoretical basis for the advanced development of cytopathological diagnostic technologies and to consolidate the minimally invasive and accurate diagnosis of cytopathologies for clinicians. Cytopathology offers many advantages over other clinical examinations, particularly for minimally invasive and accurate diagnosis.

Human pose estimate can be used in action recognition, video surveillance and other fields, which has received a lot of attentions. Since the flexibility of human joints and environmental factors greatly influence pose estimation accuracy, related research is confronted with many challenges. In this paper, we incorporate the pyramid convolution and attention mechanism into the residual block, and introduce a hybrid structure model which synthetically applies the local and global information of the image for the analysis of keypoints detection. In addition, our improved structure model adopts grouped convolution, and the attention module used is lightweight, which will reduce the computational cost of the network. Simulation experiments based on the MS COCO human body keypoints detection data set show that, compared with the Simple Baseline model, our model is similar in parameters and GFLOPs (giga floating-point operations per second), but the performance is better on the detection of accuracy under the multi-person scenes.

Aim: There is a critical need to validate biofluid-based biomarkers as diagnostic and drug development tools for traumatic brain injury (TBI). As part of the TBI Endpoints Development Initiative, we identified four potentially predictive and pharmacodynamic biomarkers for TBI: astroglial markers GFAP and S100B and the neuronal markers UCH-L1 and Tau. Materials & methods: Several commonly used platforms for these four biomarkers were identified and compared on analytic performance and ability to detect gold standard recombinant protein antigens and to pool control versus TBI cerebrospinal fluid (CSF). Results: For each marker, only some assay formats could differentiate TBI CSF from the control CSF. Also, different assays for the same biomarker reported divergent biomarker values for the same biosamples. Conclusion: Due to the variability of TBI marker assay in performance and reported values, standardization strategies are recommended when comparing reported biomarker levels across assay platforms.

Acute coronary syndrome (ACS) is a term used to describe major cardiovascular diseases, and treatment of in-stent restenosis in patients with ACS remains a major clinical challenge. Further investigation into molecular markers of ACS may aid early diagnosis, and the treatment of ACS and post-treatment recurrence. In the present study, total RNA was extracted from the peripheral blood samples of 3 patients with ACS, 3 patients with percutaneous coronary intervention (PCI)_non-restenosis, 3 patients with PCI_restenosis and 3 healthy controls. Subsequently, RNA library construction and high-throughput sequencing were performed. DESeq2 package in R was used to screen genes that were differentially expressed between the different samples. Moreover, the intersection of the differentially expressed mRNAs (DEmRNAs) and differentially expressed long noncoding RNAs (DElncRNAs) obtained. GeneCodis4.0 was used to perform function enrichment for DEmRNAs, and lncRNA-mRNA co-expression network was constructed. The GSE60993 dataset was utilized for diagnostic analysis, and the aforementioned investigations were verified using in vitro studies. Results of the present study revealed a large number of DEmRNAs and DElncRNAs in the different groups. We selected genes in the top 10 of differential expression and also involved in the co-expression of lncRNA-mRNA for diagnostic analysis in the GSE60993 dataset. The area under curve (AUC) of PDZK1IP1 (0.747), PROK2 (0.769) and LAMP3 (0.725) were all >0.7. These results indicated that the identified mRNAs and lncRNAs may act as potential clinical biomarkers, and more specifically, PDZK1IP1, PROK2 and LAMP3 may act as potential biomarkers for the diagnosis of ACS.

Image block matching is one of active research fields in image processing, which has been widely used in security monitoring and motion estimation. Due to great disparities in images of the same scene under various illumination, block matching has been a challenging task. To this end, we propose a hierarchical block matching method which is adaptive to the computational complexity and suited to complicated illumination environments. The approach is divided into two parts. First, in order to reduce searching time, the whole algorithm is established in the framework of pyramid structures. Second, the correlation coefficient and structural functions are adopted to evaluate the similarity between two images so as to get better matching results. Simulation results show that, compared with the classical three-step, four-step searching algorithms and OHBM algorithm, this algorithm can reduce the time complexity efficiently. Moreover, since the algorithm makes use of the structure details of images, it is robust to complicated scenes.

Metal-organic framework (MOFs) composites are new kind of porous materials, with great specific surface area and extremely high porosity. MOFs have been in the catalytic application due to excellent catalytic properties such as, their unique structure and dispersed active centers. Hence, the chemical, environment and energy-related fields disciplines have made substantial applications of MOFs and its composites. Moreover, several researchers have made conducted extensive studies regarding the application of MOFs materials for air pollution control such as, including functionalization, loading, carbonization, oxidation of MOFs materials, and application of MOFs as sensors for air pollution monitoring. This review article is aimed at providing precise information about the efforts made by some researchers in the field of MOFs and MOFs derived materials and their application in other disciplines. The following areas have been succinctly reviewed: (1) general introduction of MOFs materials, (2) synthesis of various MOFs materials, composites and their derivatives, (3) catalysis of MOFs materials, composites and their derivatives on different air pollution, (4) summary and future prospects. We hope this review will help researchers understand more about the research progress of MOFs materials in the catalysis of air pollution.

Background Appropriate and effective use of biological agents is important to improve the benefits of psoriasis patients. We examined how the effects of interleukin (IL) inhibitors vary across patients' demographic, social, and clinical characteristics in treating psoriasis, and whether IL inhibitors are effective for managing mild-to-moderate psoriasis. Methods Data were collected from a large national registry in China from Sep 2020 to Sep 2021. Clinical benefits, measured by 75% (and 90%) or more improvement from baseline Psoriasis Area and Severity Index (PASI 75 and PASI 90), were contrasted using the propensity-score-based causal inference methodology between the IL inhibitors and the conventional therapies. Candidates that can differentiate the benefits with P-values less than 0.05 were identified as predictors. Results Baseline PASI, DLQI, and employment demonstrated stronger predictability in achieving the benefits of IL inhibitors. For weeks 5-46, baseline PASI predicted an increasing relative benefit of biologics as the value increased from 0 to 5, followed by a V-shaped benefit as the value further increased. Baseline PASI scores at 5.4 and 1.0 predicted the maximal and minimal benefits on achieving PASI 75, with an increase in probabilities of 0.36 (95CI 0.28 to 0.44) and 0.03 (-0.14 to 0.20), respectively. Higher DLQI predicted the maximal benefit (0.55, 0.26 to 0.83) of achieving PASI 75 and lower DLQI predicted the minimal benefit of 0.13 (0.04 to 0.23). Part-time job predicted the maximal benefit of 0.28 (0.21 to 0.36) and full-time job predicted the minimal benefit of 0.15 (0.10 to 0.21). These findings were consistent in achieving PASI 75 and PASI 90. Conclusions This article fills the gap in treating mild psoriasis with IL inhibitor biologics. Patients with mild psoriasis, i.e. with PASI below 5 or BSA scores below 5, had statistically significant benefits from treatment with IL inhibitors. The studying provides evidence from real-world data on patients’ heterogeneous responses to IL inhibitor biologics. Identified clinical and social predictors can be used for treatment differentiation in clinical practice.

A probe 3 (2-ethoxy-N-(2-(2-(2-hydroxyethoxy)ethyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de] isoquinolin-6-yl)benzamide) that could selectively respond to Cr

Background Fluid therapy-the administration of fluids to maintain adequate organ tissue perfusion and oxygenation-is essential in patients admitted to the intensive care unit (ICU) with traumatic brain injury. We aimed to quantify the variability in fluid management policies in patients with traumatic brain injury and to study the effect of this variability on patients' outcomes.Methods We did a prospective, multicentre, comparative effectiveness study of two observational cohorts: CENTER-TBI in Europe and OzENTER-TBI in Australia. Patients from 55 hospitals in 18 countries, aged 16 years or older with traumatic brain injury requiring a head CT, and admitted to the ICU were included in this analysis. We extracted data on demographics, injury, and clinical and treatment characteristics, and calculated the mean daily fluid balance (difference between fluid input and loss) and mean daily fluid input during ICU stay per patient. We analysed the association of fluid balance and input with ICU mortality and functional outcome at 6 months, measured by the Glasgow Outcome Scale Extended (GOSE). Patient-level analyses relied on adjustment for key characteristics per patient, whereas centre-level analyses used the centre as the instrumental variable.Findings 2125 patients enrolled in CENTER-TBI and OzENTER-TBI between Dec 19, 2014, and Dec 17, 2017, were eligible for inclusion in this analysis. The median age was 50 years (IQR 31 to 66) and 1566 (74%) of patients were male. The median of the mean daily fluid input ranged from 1middot48 L (IQR 1middot12 to 2middot09) to 4middot23 L (3middot78 to 4middot94) across centres. The median of the mean daily fluid balance ranged from -0middot85 L (IQR -1middot51 to -0middot49) to 1middot13 L (0middot99 to 1middot37) across centres. In patient-level analyses, a mean positive daily fluid balance was associated with higher ICU mortality (odds ratio [OR] 1middot10 [95% CI 1middot07 to 1middot12] per 0middot1 L increase) and worse functional outcome (1middot04 [1middot02 to 1middot05] per 0middot1 L increase); higher mean daily fluid input was also associated with higher ICU mortality (1middot05 [1middot03 to 1middot06] per 0middot1 L increase) and worse functional outcome (1middot04 [1middot03 to 1middot04] per 1-point decrease of the GOSE per 0middot1 L increase). Centre-level analyses showed similar associations of higher fluid balance with ICU mortality (OR 1middot17 [95% CI 1middot05 to 1middot29]) and worse functional outcome (1middot07 [1middot02 to 1middot13]), but higher fluid input was not associated with ICU mortality (OR 0middot95 [0middot90 to 1middot00]) or worse functional outcome (1middot01 [0middot98 to 1middot03]).Interpretation In critically ill patients with traumatic brain injury, there is significant variability in fluid management, with more positive fluid balances being associated with worse outcomes. These results, when added to previous evidence, suggest that aiming for neutral fluid balances, indicating a state of normovolaemia, contributes to improved outcome. Copyright (C) 2021 Elsevier Ltd. All rights reserved.

Hypertension induced by hypoxia at high altitude is one of the typical symptoms of high-altitude reactions (HARs). Emerging evidence indicates that endothelial abnormalities, including increases in angiotensin-2 (Ang-2) and endothelin-1 (ET-1), are closely associated with hypertension. Thus, low blood oxygen-induced endothelial dysfunction through acceleration of Ang-2 and ET-1 synthesis may alleviate HARs. In this study, we investigated the effects of hypoxia on rat blood pressure (BP) and endothelial injury. We found that BP increased by 10 mmHg after treatment with 10% O2 (~5500 m above sea level) for 24 h. Consistently, serum Ang-2 and ET-1 levels were increased along with decreases in NO levels. In endothelial cells, angiotensin-1-converting enzyme (ACE) and ET-1 expression levels were upregulated. Interestingly, nuclear respiratory factor 1 (NRF1) levels were also upregulated, consistent with the changes in ACE and ET-1 levels. We further demonstrated that NRF1 transcriptionally activated ACE and ET-1 by directly binding to their promoter regions, suggesting that the endothelial cell dysfunction induced by hypoxia was due to NRF1-dependent upregulation of ACE and ET-1. Surprisingly, testosterone supplementation showed significant protective effects on BP, while castration induced even higher BPs in rats exposed to hypoxia. We further showed that physiological testosterone repressed NRF1 expression in vivo and in vitro and thereby reduced Ang-2 and ET-1 levels, which was dependent on hypoxia. In summary, we have identified that physiological testosterone protects against hypoxia-induced hypertension through inhibition of NRF1, which transcriptionally regulates ACE and ET-1 expression.

2D carbon ribbon/Al2O3 was synthesized with two-dimensional metal organic frameworks 2D-MOFs as precursors using the solvothermal and calcination methods. Batch experiments of adsorption parameters such as pH, liquid/solid ratio, adsorption kinetics, adsorption thermodynamics, and anions competitions were investigated to understand the adsorptive behavior of fluoride on carbon ribbon/Al2O3 and nitrogen-doped carbon ribbon/Al2O3. The adsorption of fluoride on carbon ribbon/Al2O3 could be described as the chemical and multilayer adsorption, while the adsorption of fluoride on nitrogen-doped carbon ribbon/Al2O3 followed the chemical and monolayer adsorption phenomenon. The fluoride on nitrogen-doped carbon ribbon/Al2O3 had a much faster adsorption rate of 3.1×10−7 m/s than carbon ribbon/Al2O3, which was 1.2×10−7 m/s. The nitrogen-doping on carbon ribbon enhances structural defects and improves the adsorption performance of fluoride. Also, the diacetylene linkages (—C≡C—) and pyridinic-N were studied to understand their influences on removing fluoride. The result indicates that carbon ribbon and nitrogen-doped ribbon could serve as good adsorbents for removing fluoride.

Multiple alleles were constantly detected in Alternaria solani isolates by simple sequence repeat (SSR) analysis, and sectors were also observed in their subcultures. These preliminary results and observations point to a possible parasexual cycle in A. solani. In this study, codominant SSR markers were used as molecular markers on the chromosomes of A. solani and single-conidium subculture was used to simulate the mitosis process of A. solani in nature. The number of alleles at locus As-95236 changed from 2 to 1 as a molecular marker for haploidy of parasexuality of A. solani. Fifty monosporic F1 strains were tested. The results showed that two parent strains lost allele with a haploid probability of 38%. For F2 strains, the results showed that all four F1 strains lost allele with a haploid probability of 75%. Since sexual recombination of A. solani has not been found so far, the allele lost in the subcultures of A. solani isolates provides molecular evidence for the existence of parasexual reproduction in A. solani.

Here, a novel phosphate-solubilizing bacterium (PSB), Klebsiella aerogenes Wn was applied to develop an environmental-friendly method to simultaneously stabilize Pb and Cd. The maximum dissolved phosphate was up to 701.36 mg/L by the strain Wn. The high performance liquid chromatography (HPLC) and Pearson correlation analyses showed that the acetic acid produced by the strain Wn was significantly positively associated with the released phosphate. Moreover, 100% of 500 mg/L of Pb and 100 mg/L of Cd were simultaneously stabilized in the classical NBRIP medium and the major products were Pb

Early blight (EB) disease, caused mainly by

Contains fulltext : 251563.pdf (Publisher’s version ) (Closed access) BACKGROUND: Despite being well established, acute surgery in traumatic acute subdural haematoma is based on low-grade evidence. We aimed to compare the effectiveness of a strategy preferring acute surgical evacuation with one preferring initial conservative treatment in acute subdural haematoma. METHODS: We did a prospective, observational, comparative effectiveness study using data from participants enrolled in the Collaborative European Neurotrauma Effectiveness Research in Traumatic Brain Injury (CENTER-TBI) cohort. We included patients with no pre-existing severe neurological disorders who presented with acute subdural haematoma within 24 h of traumatic brain injury. Using an instrumental variable analysis, we compared outcomes between centres according to treatment preference for acute subdural haematoma (acute surgical evacuation or initial conservative treatment), measured by the case-mix-adjusted percentage of acute surgery per centre. The primary endpoint was functional outcome at 6 months as rated with the Glasgow Outcome Scale Extended, which was estimated with ordinal regression as a common odds ratio (OR) and adjusted for prespecified confounders. Variation in centre preference was quantified with the median OR (MOR). CENTER-TBI is registered with ClinicalTrials.gov, number NCT02210221, and the Resource Identification Portal (Research Resource Identifier SCR_015582). FINDINGS: Between Dec 19, 2014 and Dec 17, 2017, 4559 patients with traumatic brain injury were enrolled in CENTER-TBI, of whom 1407 (31%) presented with acute subdural haematoma and were included in our study. Acute surgical evacuation was done in 336 (24%) patients, by craniotomy in 245 (73%) of those patients and by decompressive craniectomy in 91 (27%). Delayed decompressive craniectomy or craniotomy after initial conservative treatment (n=982) occurred in 107 (11%) patients. The percentage of patients who underwent acute surgery ranged from 5·6% to 51·5% (IQR 12·3-35·9) between centres, with a two-times higher probability of receiving acute surgery for an identical patient in one centre versus another centre at random (adjusted MOR for acute surgery 1·8; p

Connection to nature is positively associated with happiness among adults. However, whether and how connection to nature is associated with children’s happiness remains unknown. The current study aims to examine the association between connection to nature and children’s happiness and the moderating effect of temperament (negative affectivity and effortful control) on this association. Four hundred fifty-eight children aged 7–12 years and one of their parents participated in this study. Our study found that connection to nature is positively associated with both children’s self- and parent-reported happiness. Children’s negative affectivity moderates the association between connection to nature and self-reported happiness, and the moderating effect differs by gender. Specifically, the negative affectivity of girls strengthens the association between connection to nature and happiness. These findings highlight the positive impact of connection to nature on children’s happiness and the importance of considering children’s negative affectivity and gender when exploring the association between connection to nature and positive development.

Fringe projection profilometry has gained significant interest due to its high precision, enhanced resolution, and simplified design. Typically, the spatial and perspective measurement capability is restricted by the lenses of the camera and projector in accordance with the principles of geometric optics. Therefore, large-size object measurement requires data acquisition from multiple perspectives, followed by point cloud splicing. Current point cloud registration methods usually rely on 2D feature textures, 3D structural elements, or supplementary tools, which will increase costs or limit the scope of the application. To address large-size 3D measurement more efficiently, we propose a low-cost and feasible method that combines active projection textures, color channel multiplexing, image feature matching and coarse-to-fine point registration strategies. Using a composite structured light with red speckle patterns for larger areas and blue sinusoidal fringe patterns for smaller ones, projected onto the surface, which allows us to accomplish simultaneous 3D reconstruction and point cloud registration. Experimental results demonstrate that the proposed method is effective for the 3D measurement of large-size and weak-textured objects.

This paper studied laser induced thermal-crack propagation (LITP) dicing of a glass-silicon double-layer wafer with high scanning speed. A defocusing continuous laser was used in the experimental system as the volumetric heat source for the glass layer and the surface heat source for the silicon layer. Based on the principle of thermal-crack propagation, the commercial software ABAQUS was used on the simulated analysis, and the results of temperature field and thermal stress field distribution with high and low speed were compared. The experiment was executed in accordance with the simulation parameters. The surface morphology of the cut section was described by optical microscopy and a profilometer, and combined with the results, the non-synchronous propagation process of the crack under high speed scanning was revealed. Most importantly, the scanning section with a nanoscale surface roughness was obtained. The surface roughness of the silicon layer was 19 nm, and that of glass layer was 9 nm.

ImportanceUnderstanding the posttreatment prognosis of skin lesions in patients with psoriasis is essential for improving patients’ treatment satisfaction.ObjectivesTo model the prognosis of skin lesions for patients with psoriasis after 3 types of therapy.Design, Setting, and ParticipantsThis prospective cohort study included patients with psoriasis who visited a dermatologist and were enrolled in the platform of the Psoriasis Standardized Diagnosis and Treatment Center in China from August 2020 to December 2021.InterventionsBiologic, traditional, and systemic therapy for psoriasis.Main Outcomes and MeasuresSkin lesions were measured by the Investigator’s Global Assessment (IGA) scale subsumed into 4 stages of severity (IGA 0/1, IGA 2, IGA 3, and IGA 4), with higher scores indicating higher severity. The matching method was used to balance baseline covariates between patients receiving each of the 3 treatments. Transition probabilities from IGA scores at baseline to 0 to 1 month and 1 to 12 months were estimated.ResultsA total of 8767 patients were included in the final analysis (median age, 38.6 years [IQR, 28.7-52.8 years]; 5809 [66.3%] male). Across the 3 therapies, as the follow-up duration increased, the probability of improvement transition into a less severe IGA stage (from IGA 4 to IGA 0/1) increased from 0.19 (95% CI, 0.18-0.21) in 0 to 1 month to 0.36 (95% CI, 0.34-0.37) in 1 to 12 months. Biologic therapy was associated with greater improvement transitions for severe conditions, with transition probabilities from IGA 4 to IGA 0/1 increasing by 0.06 (95% CI, 0.02-0.09) vs traditional therapy and by 0.06 (95% CI, 0.03-0.09) vs systemic therapy in 0 to 1 month and by 0.08 (95% CI, 0.04-0.12) vs traditional therapy and 0.11 (95% CI, 0.07-0.14) vs systemic therapy in 1 to 12 months.Conclusions and RelevanceThis cohort study modeling psoriasis prognosis provided a complete prognosis of skin lesions, and biologic therapy was associated with improved prognosis of moderate to severe psoriasis compared with traditional and systemic therapies. The study provides insight on using transition diagrams to assess psoriasis prognosis and to communicate with patients in clinical practice.

Objective We aimed to explore the mechanisms underlying paraquat (PQ)-induced damage using cell lines (NCTC1469, TC-1, TCMK-1) and bioinformatic analysis of the GSE153959 dataset. Assessment of changes in the expression of ferroptosis-related genes in cellular damage due to paraquat poisoning and the important value of these genes in the pathogenesis. Methods Data were retrieved from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) related to ferroptosis were identified by Venn plots and analyzed for enrichment. Proteins encoded by these DEGs were studied for interactions. qRT-PCR and western blotting analyses of cultured cells were used to determine the expression of ferroptosis-related DEGs and their corresponding protein levels. Results We identified 25 DEGs primarily involved in epidermal growth factor receptor signaling, apoptotic signaling pathways, endoplasmic reticulum (ER) stress, and ferroptosis. From these, we uncovered eight ferroptosis-related DEGs, four of which were involved in ER response and regulators of ferroptosis— Chac1 (ChaC glutathione specific gamma-glutamylcyclotransferase 1), Atf3 (activating transcription factor 3), Tfrc (transferrin receptor), and Slc7a11 (solute carrier family 7 member 11). Significant changes in mRNA and protein levels of CHAC1, ATF3, TFRC, and SLC7A11 were confirmed in PQ-exposed cells. Conclusion ER stress and ferroptosis are critical for PQ-induced cell damage. CHAC1, ATF3, TFRC, and SLC7A11 are essential molecules implicated in PQ-induced ferroptosis that may serve as therapeutic targets for the amelioration of PQ poisoning.

Setting off fireworks during the Spring Festival are a popular traditional custom and recreational activity in China. Intensity of firecracker burning can quickly deteriorate air quality. Airborne bacteria are also important constituents of atmospheric particulate matter and contain many pathogens. However, few studies have reported variations in the bacterial community structure in PM2.5 during firecracker burning. In this study, PM2.5 samples were collected at four different sites in the city of Guilin during Spring Festival from February 4 to 8, 2019 and a high-throughput sequencing technology was used to investigate the variations of bacterial diversity and community structure in PM2.5. The results revealed that Proteobacteria was the predominant bacterial phylum comprising 94.3% of the total sequences, followed by Firmicutes, Actinobacteria, Bacteroidetes, and Deinococcus-Thermus. At the genus level, the top five abundant genera accounted for 81.8% of the total sequences. The proportions of major genera (Cupriavidus, Sphingobium, Brevundimonas, Sphingomonas, and Pseudomonas) were apparently affected by firecracker burning. In particular, the relative abundance of Brevundimonas genus increased dramatically from 3.2 to 60.1% at the Huaqiaonongchang (HQ) sampling site during intense firework burning, while those of other major genera and potential pathogens detected including Pseudomonas, Acinetobacter, and Serratia decreased. In addition, compared with the normal condition, the number of operational taxonomic units and the diversity and richness of microorganisms in PM2.5 samples decreased apparently. Pearson correlation results also revealed that they were negatively correlated with the NO2, SO2, and PM2.5 concentrations.