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Introduction. Sources of non-centralized water supply are the most important resource of drinking water for the population of rural areas. As a rule, water is taken from small depths, which makes it vulnerable to pollution. This type of water use is characterized by the absence of any preliminary water treatment. The constant use of groundwater for drinking can negatively affect the health of the local population since the indicators of its chemical and microbiological composition are not controlled in any way and do not always meet the quality standards. All this determines the relevance of the research conducted in the territory of the Tomsk District (Tomsk Region). Methods. Groundwater quality was assessed by comparing the concentrations of substances in the studied water samples with the background values established for the given territory in relation to Sanitary Norms and Regulations as well as microbiological indicators. Changes in the chemical and microbiological composition of the studied water, caused by economic activity, were taken as “pollution” as compared to the natural (background) state for this territory as well as sanitary and hygienic standards. Results. According to the results of studying the chemical composition of water, high levels of the following components were observed: total hardness, Na+, K+, SO42–, Cl–, NO3–, Fe, and Mn. As for NO3–, Fe, and Mn, their concentrations exceed manifold not only the background values but also the maximum allowable concentrations for drinking water. Microbiological analysis showed a large number of microorganisms from various physiological groups, which indicates a high degree of bacterial contamination of water. We present possible reasons for the input of pollutants into the water and review briefly the adverse health effects. Conclusion. In order to prevent the possible negative impact of excessive contents of various components in the chemical composition of water on the health of the local population, preliminary water treatment using available methods is recommended.

Introduction According to recent research violations of the oxidative-antioxidant balance may play an important role in the pathogenesis of schizophrenia, by changing generate, conduct and reproduce a nerve impulse. Antibodies with oxidoreductase activity may be involved in protection against oxidative stress in schizophrenia. Objectives The study was to compare the superoxide dismutase (SOD) activity of IgG in patients with acute schizophrenia and during remission. Methods The study included 20 patients with acute schizophrenia (mean PANSS total score 94,3±14), 18 people with schizophrenia during remission (mean PANSS total score 54,7±9), and 10 healthy individuals. All participants signed informed consent for the research. Isolation of IgG from blood serum was performed using affinity chromatography on Protein-G-Sepharose columns. The homogeneity of the substances is confirmed by the SDS PAGE method. SOD activity of IgG was carried out spectrophotometrically. Statistical processing was conducted with Statistica v.10. Results IgG of schizophrenia patients and healthy individuals have a SOD activity and studied activity is proved to be antibodies intrinsic property. The activity of antibodies in acute schizophrenia was 1.7 times higher than in healthy individuals (p Conclusions We can assume that in the conditions of oppression antioxidant activity in schizophrenia patients, antibodies partially take over the function of protecting the body from patients with generalized oxidative stress. This work is supported by the Russian Scientific Foundation, grant # 18-15-00053P. Disclosure No significant relationships.

Introduction Difficulties in the diagnosis of bipolar disorder (BD) are associated with a lack of understanding of the mechanisms of its pathogenesis. Identification of proteins involved in the pathogenesis of BD will bring us closer to an understanding of its mechanisms and can help in diagnosis. Objectives The search of proteomic biomarkers of bipolar disorder. Methods We performed a proteomic analysis of the serum of 16 healthy people and 33 patients with BD. Patients were hospitalized in an acute state of the depressive phase, and they did not receive therapy for more than 6 months. Blood was collected before the start of therapy. Serum was purified from major proteins by affinity chromatographyandseparatedby1D-electrophoresis. After trypsinolysis, the proteins were identified by HPLC/mass spectrometry. The ELISA kit was used to determine the amount of zNMDAR1. Results We identified a protein that does not occur in healthy people: a subunit of the glutamate NMDA receptor zeta-1 (zNMDAR1). As a result, we found a statistically significant (p = 0.037) almost fivefold increase in the concentration of this protein in the serum of patients with bipolar disorder (0.64 [0.18; 0.78] ng/ml) compared with healthy individuals. Conclusions Thus, in bipolar disorder NMDAR is damaged, which can lead appearance of their subunits in the serum, and which indicated a violation of glutamatergic neurotransmission. Then this protein claims the role of markers of bipolar disorder. Mass spectrometric analysis was carried out of the “Human Proteome” Core Facility of the Institute of Biomedical Chemistry Moscow. RSW project, state registration number AAAA-A19-119020690013-2. Disclosure No significant relationships.

The article deals with olfactory perception modus comparisons in postmodern literature (on the example of literary text by V. Pelevin: «Omon Ra», «Life of Insects», «Chapaev and Emptiness», «Generation «П», «Numbers» (as a part of «DTP»), «The Sacred Book of the Werewolf», «Empire V », «S.N.U.F.F.»). The aim of the study is to consider the olfactory perception modus comparison features in V. Pelevin's literary texts. During the study the following research methods were applied: theoretical, statistical, distributional analysis, descriptive, the method of continuous sampling. The special feature of postmodernism – the equalization of opposites – has been analyzed. The following peculiarities of olfactory perception modus comparisons in postmodernism – intertextuality, use of various types of comic means, re-thinking, rhizomatics, rejection of hierarchies – have been revealed and analyzed. The names of researchers considering these features of postmodernism are listed below: I.S. Golovanova, E.I. Dashevskaya, T.G. Prokhorova, I.S. Skoropanova. The article identifies features of rhizomatics that are expressed in fragmentation and uncertainty of comparisons. Results. The features of postmodernism manifestation in olfactory comparisons of V. Pelevin's literary texts are given. The rejection of hierarchy feature is represented by the frequency use of axiologically unmarked comparisons, rather than axiologically positively and negatively marked comparisons. The use of different types of comic means is represented by derision, ironization and impersonation of phenomena that are created by introducing comparisons into the text. Intertextuality consists of remembering the smell by a character. The feature of re-thinking the comparison of the olfactory perception modus is represented by a qualitative change in the consciousness of reality facts perception.

Lithosiini genera of the Asura / Miltochrista generic complex related to Barsine Walker, 1854 sensu lato and Asura Walker, 1854 are overviewed. Barsine is considered to be a group having such an autapomorphic feature as a basal saccular process of valva only. Many species without this process are separated to the diverse and species-rich genus Ammatho stat. nov., which is subdivided here into eight subgenera including Idopterum Hampson, 1894 downgraded here to a subgenus level, and six new subgenera: Ammathella Volynkin, subgen. nov., Composine Volynkin, subgen. nov., Striatella Volynkin & Huang, subgen. nov., Conicornuta Volynkin, subgen. nov., Delineatia Volynkin & Huang, subgen. nov. and Rugosine Volynkin, subgen. nov. A number of groups of species considered previously by various authors as members of Barsine are erected here to 20 new genera and four subgenera: Ovipennis (Barsipennis) Volynkin, subgen. nov., Ovipennis (Coccinigripennis) Volynkin & Huang, subgen. nov., Barsura (Tenebrasura) Volynkin, subgen. nov., Argentosine Volynkin, gen. nov., Esmasura Volynkin & Huang, gen. nov., Matsumursine Volynkin & Huang, gen. nov., Floridasura Volynkin, gen. nov., Fossia Volynkin, Ivanova & Huang, gen. nov., Wittasura Volynkin, gen. nov., Disparsine Volynkin, gen. nov., Moorasura Volynkin & Huang, gen. nov., Sarbine Volynkin, gen. nov., Sarbine (Processine) Volynkin, subgen. nov., Hampsonascia Volynkin, gen. nov., Cernysura Volynkin, gen. nov., Barsilene Volynkin & Huang, gen. nov., Nanarsine Volynkin, gen. nov., Amphisine Volynkin, gen. nov., Karolia Volynkin, gen. nov., Niveutane Volynkin, gen. nov., Rubrindiania Volynkin & Huang, gen. nov., Barsaurea Volynkin & Huang, gen. nov., Integrivalvia Volynkin & Huang, gen. nov. and Aberrasine Volynkin & Huang, gen. nov. The genus Nebulene Volynkin & Černý is downgraded to a subspecies of Ovipennis. The genus Eutane Walker, 1854 is downgraded to a subspecies of Asura. The genera Miltasura Roepke, 1946 and Gymnasura Hampson, 1900 are synonymised here with Cyme Felder, 1861 and Asura respectively. The genera Asuropsis Matsumura, 1927, Neasuroides Matsumura, 1927 and Asuridoides Daniel, 1951 are synonymised with Miltochrista Hübner, [1819]. The genus Allochrista Roepke, 1946 is synonymised with the subgenus Thyrgorina Walker, [1865] of the genus Lemyra Walker, 1856 (tribe Arctiini) with establishing a new combination Lemyra (Thyrgorina) toxopei (Roepke, 1946), comb. nov. Other six new synonyms are established: Barsine pardalis (Mell, 1922) = Barsine miranda Kishida & Wang, 2017, syn. nov., Barsine striata striata (Bremer & Grey, 1852) = Miltochrista quelparta Okamota, 1924, syn. nov., Floridasura tricolor (Wileman, 1910) = Barsine coccinea Moore, 1886, syn. nov., Disasuridia metaphaea (Hampson, 1900) = Disasuridia flava Fang, 1991, syn. nov., Aberrasine aberrans aberrans (Butler, 1877) = Miltochrista decussata (Moore, 1877), syn. nov. and Cabarda nigripuncta (Wileman & South, 1919) = Asura lunilinea Schaus, 1922, syn. nov. In addition, it is stated that Miltochrista quadrifasciata Rothschild, 1913 described from New Guinea and currently belonging to the genus Cyme (a junior synonym of Cyme sexualis (Felder, 1864)) is an invalid name, being a secondary junior homonym (homonym nov.) of Cyme quadrifasciata (Rothschild, 1913), comb. nov. described from Sulawesi. A full check-list of members of the Asura / Miltochrista generic complex with 370 new combinations is present.

The genus Longarista Volynkin, 2019 is reported for the first time in India. The genus is represented in India by Longarista kareli Volynkin, 2019 previously known from Vietnam, Laos, Thailand, and Myanmar. The female of L. kareli is described for the first time. Illustrations of adults and genitalia of both species of the genus are provided. Keywords: Asia, Female, India, Longarista, New record

Microfertilizers is an important factor for good production of the mycelium of Lentinula edodes mushrooms. The commercial cultivation of mushrooms depends on the correct adjustment of the components of the nutrient media. Three formulas of nutrient media, including decoctions of oak, oatmeal and potatoes. Most mushrooms grow and function well at a pH close to neutral or light basic. Method of light microscopy. Biotechnological (obtaining and subcultuvating of strain 3667 in vitro), microbiological (obtaining pure mushroom culture, studying the cultural properties of the colonies, determining the hydrogen index (pH) of the nutrient medium), mycological (measurement of growth, growth density and dry weight of mycelium, determination of mycelial radial growth, analysis for the presence of buckles and hyphae), light microscopy and statistical methods were used. Performed experiments showed that acceleration of mycelial growth and the greatest yield of mycelium L. edodes were observed on a nutrient medium that contained microfertilizer «Avatar-1». The performed experiments showed that the acceleration of mycelial growth and the greatest yield of mass of mycelium L. edodes were observed on nutrient media with microfertilizer «Avatar-1». During the experiment, it was found that the maximum overgrowth of the medium by mycelium occurs at 7 days. It has been proved that in the «Avatar-1» environments, there was an increase and consolidation of bifurcated hyphae and buckles. The dependence of growth rate on the type of nutrient medium, the administration of doses of the drug, which effectively influences and reduces the technology of obtaining primary mycelium L. edodes, is demonstrated.

The review is based on the results of the operation of the total ozone (TO) monitoring system in Russia and adjoining territories that functions in the operational mode at the Central Aerological Observatory. The monitoring system uses data from the national network equipped with M-124 filter ozonometers being under the methodological supervision of the Main Geophysical Observatory. The quality of the system functioning is operationally controlled using OMI satellite equipment observations (NASA, the USA). Basic TO observation data are generalized for each month of the third quarter of 2019 and for the third quarter. Data of routine observations of surface ozone values in the Moscow region and Crimea are also considered.

The objective of this research was to focus on a new approach to consequence assessment of a company’s technological development. In view of the impact of a company’s technical and technological development regime on the efficiency of its operation, we used the method of assessing the core business along with pairwise comparisons to assess the basic business potential of a single company and related groups. Our proposed method for evaluating the business core was based on the technique of pairwise comparisons using the work of T. Saaty along with Fishburn’s method. We analyzed the hierarchical structure of the chosen enterprises using the following metrics: products, consumers, intangible assets, distribution channels and capital. In order to identify the technological basis of the enterprise’s competitive advantages, we first initiated a decomposition of the enterprise’s technological structure: business core, periphery and prospects. We used data obtained from the machine-tool sector of Ukraine, a country which demonstrates how a lack of effective technological development along with inadequate tools for the development and implementation of technological strategies can lead to significant destructive effects on national competitiveness. In particular, we pointed to a decrease in rates of return and profitability of production. In conclusion, we argue that a passive management policy of an enterprise with regard to its technological development can lead to catastrophic consequences for competitiveness in terms of innovation and development.

Purpose: In article on the basis of the formal legal analysis of the national legal system and the international jurisprudence the key principles of state legal regulation of the confessional relations in the sphere of the general education are distinguished; the need of their addition and unification at the national level for the purpose of providing the balanced ratio of public and private interests in the context of providing the integrated rights and personal freedoms is demonstrated. Methodology: In work, various general scientific methods, acceptances, and methods of logical knowledge are used: analysis and synthesis, system, formal and logical, formal legalistic. Result: It should be emphasized that the process of legal regulation of the modern secular state is not enough to adhere to the principle of religious neutrality and minimize the amount of religious component in the public spheres of life of the individual and society, including the system of General education. Currently, such a state is designed to ensure a balance between public and private interests in this area. Applications: This research can be used for universities, teachers, and students. Novelty/Originality: In this research, the model of Legal Regulation of Interreligious Relations in the Field of General Education: The Ratio of Public and Private Interests is presented in a comprehensive and complete manner.

Mercury (Hg) and its compounds are among the most hazardous environmental pollutants with a high cumulative potential and they can have toxic effects on human and animal health even in low concentrations. Due to the increasing rate of human economic activity and the increase in the amount of Hg in the total cycling of matter, the study of its distribution, transformation, redistribution and accumulation in the abiotic and biotic components of various ecosystems remains important up to the present time. We assessed the content of metal in organs and tissues of the bank vole Myodes (Clethrionomys) glareolus Schreber, 1780 (Rodentia, Cricetidae), a widespread small rodent, caught in different biotopes of forest-steppe and steppe zones of Voronezh region. Measurements of Hg in samples were carried out with a mercury analyzer RA-915+ with the accessory PYRO (Lumex) using the atomic absorption method of cold steam without preliminary sample preparation (the lower limit of mercury detection in samples was 0.001 mg/kg). The sample size was 344 specimens. Mean Hg concentrations ranged from values below the analytical determination threshold to 0.887 mg/kg dry weight in the kidneys, 0.411 in the liver, 0.031 in the muscle tissue, and 0.040 in the brain. A positive correlation was found between the metal content in all possible pairs of organs (except for the “muscle – brain” pair) and a weak negative correlation was found between the Hg level and the mass of the animals. Hg concentrations in the studied organs did not differ between males and females. The metal content in the liver and kidneys of voles from the forest-steppe zone was significantly higher than in those from the steppe zone. Among all studied biotopes (meadow, pine and mixed forest, shrub thickets), the lowest concentrations were observed in animals living in pine forests, while the highest one – in more humidified bush thickets. During the vegetation season, there was a decrease in the average values of animal body mass in the samples and an increase in the content of Hg in the liver and kidneys. The results of the study are relevant in the assessment of atmospheric mercury pollution of terrestrial ecosystems using small mammals, such as Myodes glareolus, as a model object.

Materials science studies the relationship between the structure and properties of materials and their changes under external impacts, and it belongs to the priority domains of science and technology. The creation of new materials, improvement of existing materials, and development of efficient technologies for their processing are the main goals of materials science. Today a substantial number of new metal alloys with special properties, different composites, ceramics, polymers, nanostructured functional powder and synthetic superhard materials, multifunctional coatings, etc., have been designed for various economic activities. But the creation of materials and technologies remains an urgent need because of the progressive development of manufacturing which requires adaptive properties of materials and technologies for their processing. This review analyzes the directions of research at North-Eastern Federal University in the field of tribotechnical materials for use in the Arctic regions. The main areas of research are identified, namely, development of new technologies of production of polymer composite materials (PCM) and improving their performance properties. The prospects of physical processing and chemical modification of the polymer matrix surface are shown. Physicomechanical and tribotechnical properties of antifrictional PCM are discussed. The results of the main theoretical generalizations in the field of analysis of PСM supramolecular structures are presented: (1) effects of critical filler concentrations on the structure and properties of PCM; (2) hypothesis of the nature of intermolecular interaction between the filler and the polymer. The modern trends of scientific investigation are shown, one of which is tribooxidative processes in PCM. The specific development of scientific knowledge and the use of polymers for the northern regions are revealed.

Water sorption on cellulose was studied in detail. The mechanism of the adsorption–desoprtion hysteresis loop was considered using NMR relaxation methods and sorption phenomena. The degree of crystallinity was found to decrease in cellulose during water sorption, while the specific surface area of cellulose increased because a wedging pressure arose as a new adsorbent–adsorbate interface formed. The most significant changes in these parameters were observed during desorption and were accompanied by the formation of an additional capillary-porous system in cellulose. Correlations were observed between the surface tension coefficient at the solid–liquid interface, the adsorption equilibrium constant, and the net heat of adsorption.

The ability of cerium(IV) hydrogen phosphate Ce(PO4)(HPO4)0.5(H2O)0.5 to adsorb radionuclides was studied. The degree of sorption from aqueous solutions exceeds 95% at pH > 7 for Np(V), at pH > 9 for Sr(II), at pH > 5 for Am(III) and U(VI), and at pH > 2 for Th(IV). The radionuclides are rapidly and quantitatively leached from the sorbent surface in acid solutions; i.e., the sorption is reversible.

Purpose of the study: The urgency of the problem under study is due to the need to ensure the substantial unity of the two stages of vocational education: undergraduate and graduate. In connection with the increase in the proportion and importance of independent learning activities of students, the problem of self-regulation and self-control of students in the organization of this process and the provision of conditions for the formation of educational autonomy of bachelors and undergraduates from the side of university teachers becomes more acute. The aim of the article is to determine the principles and effective technologies of organizing activities for the formation of the educational autonomy of students in a two-level education. Methodology: The leading principles of the study were the principles of human-like education and student-centered education. Results: The authors formulated the principles, presented copyright technology to ensure the educational autonomy of students. The results of the use of the technological complex in the framework of the educational activities of physical culture students are indicated. The created scientific and methodological complex allowed to improve the level of self-organization and self-government of students in educational activities. Applications of this study: Materials may be applied to solve a wide range of vocational education tasks. The results of the study may be applied by teachers of higher education in order to develop the professional creativity of students and the formation of their professional identity. Novelty/Originality of this study: The problem of the formation of the educational autonomy of students is exacerbated in the conditions of modern higher education due to the increasing proportion of independent work of students and the possibilities of an open educational environment. The originality of the research lies in the development of technology for the use of anthropic educational technologies, focused more on students' activities, rather than regulating the activities of university teachers.

The purpose of the article: This was aimed at conducting a system analysis of the forms of interaction between religious organizations and modern states implementing the secular model regarding the regulation of religion component in the field of general education.Materials and methods: Various general scientific methods and methods of logical cognition were used in this study including analysis and synthesis, systemic, formal-logical. The subject under study was investigated by the use of a formal legal method and content analysis.Results of the research: The necessity with respect to the role of the state to guarantee activities of confessional educational organizations implementing general education programs (their components) in legal, organizational, methodological and financial spheres were substantiated. It was proposed to unify the approach according which the state regulating the participation of religious organizations in the field of general education in order to ensure equal opportunities for students in implementation of freedom of conscience and religion. Applications: This research can be used for the universities, teachers, and students. Novelty/Originality: In this research, the model of investigation of the role of religious organizations in system of general education: forms of state-confessional interaction is presented in a comprehensive and complete manner.

Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease that combines various clinical manifestations and pathophysiological mechanisms. It underlies the separation of patients with COPD by phenotypes, endotypes and a personalized therapy of this disease. The implementation of this approach is possible only with the use of appropriate biomarkers. One of the most important biomarkers of COPD is eosinophilia of blood and/or sputum, which is considered as a predictor of frequent exacerbations and the effectiveness of inhaled glucocorticosteroids in patients with COPD. The literature discusses the impact of eosinophilic inflammation on the prognosis, clinical and functional parameters in COPD, and the role of the targeted therapy in the treatment of eosinophilic COPD.Хроническая обструктивная болезнь легких (ХОБЛ) - гетерогенное заболевание, сочетающее в себе различные клинические проявления и патофизиологические механизмы, что лежит в основе разделения больных ХОБЛ по фенотипам, эндотипам и персонифицированной терапии этого заболевания. Реализация данного подхода возможна только при использовании соответствующих биомаркеров. Одним из наиболее важных биомаркеров при ХОБЛ является эозинофилия крови и/или мокроты, рассматриваемая как предиктор частых обострений и эффективности ингаляционных глюкокортикостероидов у пациентов с ХОБЛ. В литературе обсуждаются вопросы влияния эозинофильного воспаления на прогноз, клинические и функциональные показатели при ХОБЛ, а также роли таргетной терапии в лечении эозинофильной ХОБЛ.

SUMMARY The molecular basis of higher regenerative capacity of cold-blooded animals comparing to warm-blooded ones is poorly understood. Although this difference in regenerative capacities is commonly thought to be a result of restructuring of the same regulatory gene network, we hypothesized that it may be due to loss of some genes essential for regeneration. We describe here a bioinformatic method that allowed us to identify such genes. For investigation in depth we selected one of them encoding transmembrane protein, named “c-Answer.” Using the Xenopus laevis frog as a model cold-blooded animal, we established that c-Answer regulates regeneration of body appendages and telencephalic development through binding to fibroblast growth factor receptors (FGFRs) and P2ry1 receptors and promoting MAPK/ERK and purinergic signaling. This suggests that elimination of c-answer in warm-blooded animals could lead to decreased activity of at least two signaling pathways, which in turn might contribute to changes in mechanisms regulating regeneration and telencephalic development., Graphical Abstract Poor regeneration in warm-blooded animals could be caused by gene loss in evolution. Here, we describe a bioinformatic approach to identify lost genes. One of these, c-answer, regulates regeneration and brain development in cold-blooded animals. Thus, loss of c-answer could promote evolutionary changes related to regeneration and brain development in warm-blooded animals.

Radiation therapy with heavy particles including neutrons, an otherwise therapeutically perspective because of its high tissue penetration and efficient tumor damage, is currently limited by the lack of adequate equipment. An NG-24 generator (140 kg, 42 110 cm, ~1011 particles/s, 14 MeV) has been designed and engineered to replace the huge and environmentally harmful neutron reactors, cyclotrons, and accelerators with a compact, portable, safe, and potent source of high-energy neutrons. We demonstrate that the neutron beam produced by NG-24 causes a significant antiproliferative effect on human tumor cell lines regardless of the status of the anti-apoptotic p53 protein. Phosphorylation of histone 2A and increased amounts of p21, cyclin D, and phospho-p53 were detectable in HCT116 colon carcinoma cells (wild-type p53) irradiated with 4 Gy several days post-treatment, accompanied by G2/M phase arrest. These treatments dramatically reduced the ability of single cells to form colonies. In the HCT116p53KO subline (p53 -/-), the G2/M arrest was independent of the aforementioned mechanisms. Hence, the NG-24 generator is a source of a powerful, therapeutically relevant neutron flux that triggers a p53-independent antiproliferative response in tumor cells.

Reversible photochromic hybrid organic–inorganic films containing nanocrystalline cellulose as a matrix and tungsten oxide as a photochromic component (CNC/WO3) were obtained via a simple and quick solvent casting method. The films were studied by scanning electron microscopy, together with element mapping, FT-IR spectroscopy and X-ray diffraction, confirming successful incorporation of WO3 nanoparticles into a nanocellulose matrix. Thermal analysis data indicated that the modification of a nanocellulose matrix with WO3 increases its thermal stability. The CNC/WO3 films showed a quick coloration-bleaching transition with good reversibility within 20 min, without notable degradation of photochromic properties after 10 cycles. The synthetic method proposed allows for scalable preparation of highly efficient low-cost WO3-based photochromic materials.

The temperature evolution of the dielectric spectra of TGS–TGS + Cr crystals (TGS stands for triglycine sulfate) with a periodic distribution of chromium impurity (Cr3+) has been studied in comparison with nominally pure TGS crystals in the frequency range from 10 to 107 Hz upon heating and cooling. It is shown that impurity crystals are characterized by the absence of dielectric dispersion in both measurement modes. An approach is proposed to explain the peculiarities of the dielectric dispersion of crystals with a periodic distribution of chromium impurity from the standpoint of mobile activity of certain elements of the TGS crystal structure.

The review is based on the results of the operation of the system of total ozone (TO) monitoring over Russia and neighboring territories that functions in the operational mode at the Ceniral Aerological Observatory (CAO). The monitoring system uses data from the national network equipped with M-124 filter ozonometers being under the methodological supervision of the Main Geophysical Observatory. The quality of the entire system functioning is operationally controlled by the comparison with the observations obtained from the OMI satellite equipment (NASA, USA). Basic TO observation data are generalized for each month of the second quarter of 2019 and for the second quarter. Data of routine observations of surface ozone values in the Moscow region and Crimea are also considered.

Clutter filtering plays an important role in constructing a quality color flow map in ultrasound Doppler imaging. Signals from slow-moving tissues and vessel walls are clutter as they often mix with reflections from blood and should be suppressed for the further correct estimation of flow parameters. Their complete suppression in color flow imaging is difficult, because these signals on average are 40-60 dB more powerful than the signals from blood, the length of the Doppler sequence is very short, and there is always a demand for a real-time operation. This article provides a general model of the Doppler signal and discusses filters based on polynomial and adaptive regression, empirical mode decomposition, and prospective combined approaches to blood flow filtering.

A layered model of the structural organization of macrofibrils of native cellulose, consisting of microfibrils, which include elementary fibrils, has been developed. A feature of the proposed model is the presence of slit-like pores between the crystalline elements of cellulose. It was found that, on average, each water molecule interacts with one glucose residue of the surface chains of cellulose with the formation of hydrogen bonds in the framework of monolayer adsorption. This allows to establish a correlation between the cellulose crystallinity and the capacity of the adsorption water monolayer on its active surface. Based on the condition of rapid molecular exchange between the adsorption water layers in the framework of the Bloembergen-Purcell-Pound theory, an approach is proposed for determination the capacity of water monolayer. The obtained values are consistent with the results of solving the Brunauer-Emmett-Teller equation for the adsorption isotherm of water on the active surface of cellulose. The Fourier transform of the free induction decay signal of cellulose allows to estimate its crystallinity at various moisture contents. Methods have been developed for assessing the crystallinity of different types of dry cellulose based on NMR relaxation parameters — spin-lattice relaxation time and spin-spin relaxation time. Using the method of deuteration of cellulose, the relaxation times of its crystalline regions were determined. The results of preliminary studies showed that the crystallinity of cotton cellulose is higher in comparison with the same parameter of woody types of cellulose. A comparison of the literature and the data we obtained using 1H-NMR relaxation confirmed the possibility of utilizing the developed methods to solve the tasks of scientific research and conducting quality control of cellulosic materials at specialized enterprises.

The article considers filtering techniques used to suppress clutter signals from moving tissues and to improve reliability of blood flow estimation. It compares polynomial and adaptive bases such as the result of empirical mode decomposition and singular vectors obtained through Karhunen−Loeve transform. Filtering techniques are examined using a computer-simulated model, Doppler flow phantom and in vivo data. Filters are compared in terms of computational complexity, ability to retrieve flow profile without errors and through ROC curve analysis. Polynomial regression filters with tissue phase shift compensation were found to be the best fit for clutter suppression in terms of computational demands and accuracy of velocity estimation.

The article conducts the analysis of the practice in the application of patient registers with implanted medical devices by regulatory authorities of various countries for vigilance of medical devices, as well as review of current patient registers their categories and applicability. In addition, the article review the recommendations of The International Medical Device Regulators Forum (IMDRF) on development, implementation and application methodology of patient registers.

Hydrogen peroxide is widely used for disinfection purposes by food industry enterprises. Apart from other purposes it is also used to sterilize the packaging of dairy products. In some countries hydrogen peroxide is added to milk as an adulterant for shelf life extension. However, in most countries the concentration of hydrogen peroxide in milk is limited or it is even prohibited to add this substance to the product. This study is the first one to describe the method of determination of hydrogen peroxide in milk using high performance liquid chromatography. Indirect quantitative evaluation of hydrogen peroxide is carried out according to the amount of triphenyl oxide produced after the reaction between hydrogen peroxide with triphenylphosphine. It has been shown that interaction of triphenylphosphine and hydrogen peroxide required about 40 min. The detection limit of this method is 0.28 mg/L. The recovery of hydrogen peroxide was from 97.8% to 103.8%.

The review is compiled of the results of the total ozone (TO) monitoring in the CIS and Baltic countries that functions in the operational mode at the Central Aerological Observatory (CAO). The monitoring system uses data from the national net work equipped with M-124 filter ozonometers op er at -ing under the methodological supervision of the Main Geophysical Observatory. The quality of the functioning of the entire system is operation ally con trolled in CAO by the comparison with the observations obtained from the OMI satellite equipment (NASA, USA). Basic TO observation data are generalized for each month of the first quarter of 2019 and for the first quarter. Data of routine observations of surface ozone values in the Moscow region and Crimea are also considered.

Modernized model of microfibril cellulose layered structure is proposed. This model considers presence of slit-shaped micropores in space between elementary fibrils and cellulose microfibrils. It’s discussed the nature of donor-acceptor hydrogen bonds formation: intra-, intermolecular, and interlayer bonds inherent in each glucopyranous cellulose link. It’s described the mechanism of water molecules specific adsorption interactions in a monolayer with active centers located on the hydrophilic surfaces of elementary fibrils. Dipole-dipole energy transition into energy of hydrogen bond is discussed during adsorption process between active centers of cellulose and water adsorptive molecules. Analysis of water molecules dipole-dipole interactions with surface hydroxyl groups of cellulose showed that at distance of 2.5-3 Å energy of this interaction transforms into energy of hydrogen bond. It is discussed the formation mechanism of water molecules donor-acceptor bonds with cellulose surface hydroxyl groups. Thermodynamic parameters characterizing adsorbate state the in these layers are determined by proton magnetic relaxation and sorption measurements. It’s established the possibility of determining adsorption net heat in bilayer considering Arrhenius nature of adsorbate thermal molecular motions correlation times. Increase in entropy of adsorbed water during adsorption process is revealed basis on Vant Hoff equation and certain adsorption equilibrium constant. The calculation established that distance between nearest active centers of cellulose is 6.5 Å. This leads to disunity of adsorbed water molecules and allows application of Langmuir and BET adsorption theory. Analysis of spin-lattice relaxation times dependence on cellulose moisture content made it possible to establish the cause of its crystallite wedging from adsorbed water molecules at adsorption initial stages. Decline of the spin-lattice relaxation unambiguously indicates the process of cellulose dispersion into its structural elements. It was established that during adsorption a part of the internal regions of crystallites passes to their surface with participation of cellulose hydroxyl groups. During desorption reverse process is observed.

Aim. To perform an arterial hypertension (HT) screening for assessing the awareness of Moscow Region citizens about high blood pressure (BP) and draw their attention to this important risk factor. Material and methods . The screening was performed as a part of Standardized International Screening Study "May Measurement Month 2018” in five towns of Moscow Region: Domodedovo, Balashikha, Mytishchi, Solnechnogorsk, Klin. 510 participants (324 females, 186 males) were involved, aged from 18 to 86 years (median age 42 [28;57] years). People over 1 8 years were asked to measure BP levels and fill out a questionnaire: age, gender, height and weight, previous BP measurement date, bad habit (tobacco smoking, alcohol consumption), cardiovascular diseases and type 2 diabetes history, previous high BP levels and antihypertensive drugs taking. Elevated BP was considered systolic BP (SBP) ≥ 140 mm Hg and/or diastolic BP (DBP) ≥ 90 mm Hg. Mechanical and automatic tonometers were used. Results. Increased BP was registered in 24% of participants. High BP levels were more common among men of working age and women older than working age. Increased BP was registered for the first time in 7% of the participants, and half of the participants who had previously taken antihypertensive therapy had increased BP levels. The highest prevalence of elevated BP levels was detected in obese men (55%). Conclusion. Regular preventive medical examinations have the task of health keeping and timely disease prevention. The continuity in the medical care may reduce the incidence of socially significant diseases.

The article presents data on the mercury content in the muscles of perch Perca fluviatilis (L.) in four large reservoirs of the Vologda region (Kubenskoe and Vozhe lakes, river and lake (Lake Beloe) parts of the Sheksna reservoir) collected during the period 2007–2018. The metal concentration varied widely: from 0,01 mg/kg for perch from Lake Vozhe to 1,51 mg/kg for perch from Lake Kubenskoe. The highest percentage of fish with mercury content exceeding the established SanPiN 2.3.2.1078-01 norms (29,4%) was recorded in perch from Lake Kubenskoe, while the smallest – in fish from Lake Vozhe (5,4%). In fish from the Sheksna reservoir the content of the toxicant in muscles did not go beyond the accepted SanPiN 2.3.2.1078-01 norms. For all perch samples, a statistically significant positive correlation between mercury content and linear-weight characteristics, as well as fish age, was established.

Scleroderma is a multiorgan disease characterized by progressive lesions of the connective tissue with a predominance of fibro-sclerotic and vascular disorders in the skin, musculoskeletal system, and also in the internal organs. The observation of the bullous form of localized scleroderma and scleroatrophic lichen is presented.

The aim of this paper is to define the set guidelines of conception of quality of life and its components, to present the results of the evaluation of quality of working life in an organisation, and to disclose discoveries of the study of the work-family conflict and the balance of work and personal life. This paper follows the analysis of scientific and methodological literature, the methodology of social research and a case study. A model of quality of life was designed, which includes eight domains of quality of life and their factors. This model was used to study the quality of working life in one particular company. The research findings show that studied organization has possibilities to increase their employee’s quality of life and loyalty by improving working conditions and environment. The findings suggest that designed research method is suitable for evaluation of quality of working life and quality of life. It is possible to generalize, that quality of life and quality of working life can be managed, measured and evaluated. Using “The reason for the intention to quit work model” (J.C. Rode, 2007), the research of balance of work and personal life was conducted in a form of a survey in which there participated representatives of two extremely distinct groups according to the nature of their work: creative workers and office workers. It was found that a different nature of work really determines a different work-life balance from the objective point of view. However, hardly has any influence towards the considered work-life balance of the exploratory groups themselves from a subjective point of view. It appears, even though both the creative and office workers are satisfied with their work-life balance, respectively, yet from the objective point of view, personal life of the creative workers suffers from work much more.

Currently, women-specific risk factors for cardiovascular diseases (CVD) are of particular importance, one of which is hypertensive disorders during pregnancy (HDP). The effect of hypertensive disorders on the formation of arterial stiffness as a predictor of CVD development remains insufficiently studied. Aim. To study the relationship of indicators of arterial stiffness, risk factors and metabolic changes with the presence of HDP in the history of women of different age groups. Materials and methods. The study included women aged 30 to 65 years, with one or more risk factors of CVD, which were divided into two groups: 1st - with preserved menstrual function and 2nd group - in the postmenopausal period with the presence or absence of HDP. All patients underwent daily blood pressure monitoring with determining the dynamics of central arterial pressure, determining the velocity of the pulse wave from the carotid to the femoral arteries, non-invasive determination of vascular stiffness indicators using the volume sphygmography method. Results. 90 women were examined. The first group consisted of 45 women (mean age 41±5.9 years); the second group - 45 women (mean age 55.3±5.8 years). In both groups, women with HDP are more likely to have burdened CVD heredity (p=0.02). In the 1st group with HDP, the average body weight and body mass index were significantly higher (p=0.02); menstrual disorders occurred more than twice as often (p=0.01) than in women without HDP. In the 2nd group with HDP abortions were more frequent (p=0.05) in history. In the 1st group with HDP, a significantly higher augmentation index AI (p=0.01); the average value of the average daily rate of the pulse wave in the aorta was also exceeded (11.7 m/s), which is 0.8 m/s higher than in women without HDP (p=0.02). Conclusion. A change in the AI and the average daily pulse wave velocity in the aorta, along with the presence of CVD risk factors and hormonal and metabolic disorders, are early markers of subclinical damage to target organs in women of reproductive age with a history of HDP.

An Erratum to this paper has been published: https://doi.org/10.1134/S0016702922190016

Pluripotent stem cells (PSCs) hold great potential both in studies on developmental biology and clinical practice. Mitochondrial metabolism that encompasses pathways that generate ATP and produce ROS significantly differs between PSCs and somatic cells. Correspondingly, for quite a long time it was believed that the redox homeostasis in PSCs is also highly specific due to the hypoxic niche of their origin—within the pre-implantation blastocyst. However, recent research showed that redox parameters of cultivated PSCs have much in common with that of their differentiated progeny cells. Moreover, it has been proven that, similar to somatic cells, maintaining the physiological ROS level is critical for the regulation of PSC identity, proliferation, differentiation, and de-differentiation. In this review, we aimed to summarize the studies of redox metabolism and signaling in PSCs to compare the redox profiles of pluripotent and differentiated somatic cells. We collected evidence that PSCs possess metabolic plasticity and are able to adapt to both hypoxia and normoxia, that pluripotency is not strictly associated with anaerobic conditions, and that cellular redox homeostasis is similar in PSCs and many other somatic cells under in vitro conditions that may be explained by the high conservatism of the redox regulation system.

Fe3O4@SiO2 core-shell nanoparticles (NPs) were synthesized with the co-precipitation method and functionalized with NH2 amino-groups. The nanoparticles were characterized by X-ray, FT-IR spectroscopy, transmission electron microscopy, selected area electron diffraction, and vibrating sample magnetometry. The magnetic core of all the nanoparticles was shown to be nanocrystalline with the crystal parameters corresponding only to the Fe3O4 phase covered with a homogeneous amorphous silica (SiO2) shell of about 6 nm in thickness. The FT-IR spectra confirmed the appearance of chemical bonds at amino functionalization. The magnetic measurements revealed unusually high saturation magnetization of the initial Fe3O4 nanoparticles, which was presumably associated with the deviations in the Fe ion distribution between the tetrahedral and octahedral positions in the nanocrystals as compared to the bulk stoichiometric magnetite. The fluorescent spectrum of eosin Y-doped NPs dispersed in water solution was obtained and a red shift and line broadening (in comparison with the dye molecules being free in water) were revealed and explained. Most attention was paid to the adsorption properties of the nanoparticles with respect to three dyes: methylene blue, Congo red, and eosin Y. The kinetic data showed that the adsorption processes were associated with the pseudo-second order mechanism for all three dyes. The equilibrium data were more compatible with the Langmuir isotherm and the maximum adsorption capacity was reached for Congo red.

Fe3O4@SiO2 core-shell nanoparticles (NPs) were synthesized with the co-precipitation method and functionalized with NH2 amino-groups. The nanoparticles were characterized by X-ray, FT-IR spectroscopy, transmission electron microscopy, selected area electron diffraction, and vibrating sample magnetometry. The magnetic core of all the nanoparticles was shown to be nanocrystalline with the crystal parameters corresponding only to the Fe3O4 phase covered with a homogeneous amorphous silica (SiO2) shell of about 6 nm in thickness. The FT-IR spectra confirmed the appearance of chemical bonds at amino functionalization. The magnetic measurements revealed unusually high saturation magnetization of the initial Fe3O4 nanoparticles, which was presumably associated with the deviations in the Fe ion distribution between the tetrahedral and octahedral positions in the nanocrystals as compared to the bulk stoichiometric magnetite. The fluorescent spectrum of eosin Y-doped NPs dispersed in water solution was obtained and a red shift and line broadening (in comparison with the dye molecules being free in water) were revealed and explained. Most attention was paid to the adsorption properties of the nanoparticles with respect to three dyes: methylene blue, Congo red, and eosin Y. The kinetic data showed that the adsorption processes were associated with the pseudo-second order mechanism for all three dyes. The equilibrium data were more compatible with the Langmuir isotherm and the maximum adsorption capacity was reached for Congo red.

Gd-based complexes are widely used as magnetic resonance imaging (MRI) contrast agents. The safety of previously approved contrast agents is questionable and is being re-assessed. The main causes of concern are possible gadolinium deposition in the brain and the development of systemic nephrogenic fibrosis after repeated use of MRI contrasts. Thus, there is an urgent need to develop a new generation of MRI contrasts that are safe and that have high selectivity in tissue accumulation with improved local contrast. Here, we report on a new type of theranostic MRI contrast, namely dextran stabilised, gadolinium doped cerium dioxide nanoparticles. These ultra-small (4-6 nm) Ce

The development of cell-based approaches to the treatment of various cornea pathologies, including limbal stem cell deficiency (LSCD), is an area of current interest in regenerative biomedicine. In this context, the shortage of donor material is urgent, and limbal mesenchymal stem cells (L-MSCs) may become a promising cell source for the development of these novel approaches, being established mainly within the rabbit model. In this study, we obtained and characterized rabbit L-MSCs and modified them with lentiviral transduction to express the green fluorescent protein EGFP (L-MSCs-EGFP). L-MSCs and L-MSCs-EGFP express not only stem cell markers specific for mesenchymal stem cells but also ABCG2, ABCB5, ALDH3A1, PAX6, and p63a specific for limbal epithelial stem cells (LESCs), as well as various cytokeratins (3/12, 15, 19). L-MSCs-EGFP have been proven to differentiate into adipogenic, osteogenic, and chondrogenic directions, as well as to transdifferentiate into epithelial cells. The possibility of using L-MSCs-EGFP to study the biocompatibility of various scaffolds developed to treat corneal pathologies was demonstrated. L-MSCs-EGFP may become a useful tool for studying regenerative processes occurring during the treatment of various corneal pathologies, including LSCD, with the use of cell-based technologies.

Successful implantation requires two-sided interaction between genetically normal embryo and properly prepared endometrium. At the cellular level the latter means hormone-regulated differentiation of endometrial stromal cells (ESCs) into decidual ones that create appropriate microenvironment for invading embryo. Impaired decidualization is proved to mediate implantation failures. Here we elicited ESCs’ senescence as the cause for disturbed decidualization of endometrial stroma and impaired blastocyst implantation. Ability to decidualize and to accept modeled blastocysts inversely correlated with senescence in patients’ ESCs lines. Reduced hormonal responsiveness of senescent ESCs led to inappropriate decidualization dynamics resulting in altered receptivity, disturbed ligand-receptor interaction with trophoblasts and modified architecture of extracellular matrix what hindered blastocysts’ invasion. Furthermore, senescent ESCs caused ‘bystander’ quenching of decidual reaction in adjacent cells reinforcing dysfunction of stromal compartment. Implementation of senomorphics reducing senescence phenotype diminished adverse effects of senescent ESCs on decidualization and implantation using both in vitro models and patients’ lines, what suggests a promising strategy to increase in vitro fertilization efficacy.

Synoecnemahirsutum Timm, 1959 (Ungellidae, Drilonematoidea) found in the body cavity of the pheretimoid earthworm at the border of Laos and Vietnam was redescribed and illustrated. It was molecularly characterised for the first time. It is also the first member of the superfamily Drilonematoidea for whom the mitochondrial genome was obtained.