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Sulfated polysaccharides (SPs), especially those with immunostimulant effects on shrimp and fish, are bioactive compounds with potential use in aquaculture. In this study, Pacific white shrimp (Penaeus vannamei) were fed diets containing 0%, 0.5%, 1.0%, 1.5%, or 2.0% crude extracts from the microalga Porphyridium cruentum for 30 days. After dietary supplementation, the shrimp were exposed to Vibrio alginolyticus, allowing the assessment of phenoloxidase activity (PO) and total hemocyte count (THC) pre- and post-challenge, as well as shrimp survival rates. Shrimp that received the 1.0% treatment had greater survival (90%) at 48 h post-challenge, while the 0% and 2.0% treatments had survival rates close to 63% in the same period. Furthermore, at 48 h post-challenge, THC was significantly greater (p < 0.05) in the shrimp that received crude extract supplementation compared to that in shrimp of the control group (0%). PO showed a proportional increase according to crude extract inclusion levels. Such dose-dependency in response to PO increases became significantly greater (p < 0.05) in the 2.0% treatment group compared to that in the 0% treatment group in the pre-challenge period and that in the 0% and 0.5% treatment groups in the post-challenge period. These results showed that crude extracts from P. cruentum could be considered immunostimulants by increasing the resistance of P. vannamei exposed to V. alginolyticus, potentially representing a major breakthrough for marine shrimp farming. [ABSTRACT FROM AUTHOR]

In aquaculture, the presence of dead fish on the water surface can serve as a bioindicator of health issues or environmental stressors. To enhance the precision of detecting dead fish floating on the water's surface, this paper proposes a detection approach that integrates data-driven insights with advanced modeling techniques. Firstly, to reduce the influence of aquatic disturbances and branches during the identification process, prior information, such as branches and ripples, is annotated in the dataset to guide the model to better learn the scale and shape characteristics of dead fish, reduce the interference of branch ripples on detection, and thus improve the accuracy of target identification. Secondly, leveraging the foundational YOLOv8 architecture, a DD-IYOLOv8 (Data-Driven Improved YOLOv8) dead fish detection model is designed. Considering the significant changes in the scale of dead fish at different distances, DySnakeConv (Dynamic Snake Convolution) is introduced into the neck network detection head to adaptively adjust the receptive field, thereby improving the network's capability to capture features. Additionally, a layer for detecting minor objects has been added, and the detection head of YOLOv8 has been modified to 4, allowing the network to better focus on small targets and occluded dead fish, which improves detection performance. Furthermore, the model incorporates a HAM (Hybrid Attention Mechanism) in the later stages of the backbone network to refine global feature extraction, sharpening the model's focus on dead fish targets and further enhancing detection accuracy. The experimental results showed that the accuracy of DD-IYOLOv8 in detecting dead fish reached 92.8%, the recall rate reached 89.4%, the AP reached 91.7%, and the F1 value reached 91.0%. This study can achieve precise identification of dead fish, which will help promote the research of automatic pond patrol machine ships. [ABSTRACT FROM AUTHOR]

As the world's largest producer of fish, China has faced significant challenges in developing its fisheries. Over the past four decades, China has implemented and adjusted multiple regulations and measures to reverse the decline of aquatic resources and achieve sustainable fisheries. This study analyzes the production trends and efforts during the period 1980–2019, highlighting the key features of and structural shifts in China's fisheries. Our results reveal a significant shift in seafood production from capture to aquaculture and from offshore to inshore waters. Moreover, China's fishery policy has transitioned from resource exploitation to ecological conservation, with aquaculture production in 2019 being nearly 38 times higher than that in 1980. However, not all the management measures have been successful; for example, the "Dual Control" policy failed to achieve its goals. Despite this, some measures that require adjustments, such as the "Aquatic Germplasm Resource Reserves" and "Nature Reserve for Aquatic Animals and Plants" policies, show promise for further improving the sustainability of China's fisheries, particularly if the focus is on marine resources. [ABSTRACT FROM AUTHOR]

In prawn farming, the main protein source used in aquafeed formulations is fishmeal. Nevertheless, one estimates that in the coming years, this protein source will no longer be able to meet the demand for the activity. The search for new ingredients is important to meet the increasing demand and minimize environmental impacts, such as the reduction in fish stocks and the eutrophication of aquatic systems. The objective of this study was to determine the apparent digestibility coefficients of dry matter (DM), crude protein (CP), gross energy (GE), and ether extract (EE) of fishmeal, poultry co-products (viscera and hydrolysed feather), and insect meal (Gromphadorhina portentosa) for giant river prawn (Macrobrachium rosenbergii) juveniles. To determine the apparent digestibility coefficients (ADCs), 90 prawns (average weight, 15 g) were randomly distributed among three experimental units. The reference feed was formulated according to the requirements of the giant river prawn, with 35% crude protein and a gross energy of 3600 kcal kg−1. The test diets comprised 70% of the reference diet and 30% of the respective test ingredients. Prawns were fed three times a day until apparent satiety. Faeces were collected using the indirect siphoning method, twice a day at the same feeding site (at 7:30 a.m. and 6:30 p.m.). The water parameters were temperature (27 °C), dissolved oxygen (6.65 mg L−1) and pH (7.76). The ACDs of DM, CP, EE, and GE were, respectively, 61.48; 88.28; 99.89 and 88.25 for fishmeal; 76.48; 81.55; 97.29 and 85.13 for poultry viscera meal; 73.82; 75.21; 73.17 and 76.42 for hydrolysed feather meal; and 52.35; 59,48; 87.95 and 67.64 for G. portentosa meal. The values of protein (%) and digestible energy (kcal kg−1) were 55.20 and 3711 for fishmeal; 47.27 and 4285 for poultry viscera's meal; 65.03 and 4145 for hydrolysed feather meal; and 47.72 and 3616 for G. portentosa meal. These results showed the potential use of insect meals and poultry co-products as ingredients for the diets of M. rosenbergii juveniles, as they present digestible values close to those found for fishmeal, the main raw material used in aquaculture diets. [ABSTRACT FROM AUTHOR]

(1) Background: Genotype-by-environment interaction (G×E) can adversely impact genetic improvement programs. The presence of G×E is mainly measured as the genetic correlation between the same trait measured in different environments where departure from unity can be taken as presence of G×E. (2) Methods: To understand the extent of G×E in shrimp production, a review and meta-analysis was conducted using the results from 32 peer-reviewed studies. (3) Results: Of these, 22 G×E studies were conducted on Pacific white shrimp (Litopenaeus vannamei) with fewer studies reported in other shrimp species. The most frequently studied traits were growth and survival, with relatively few studies on traits of economic importance. The meta-analysis demonstrated a moderately high genetic correlation (rg = 0.72 ± 0.05) for growth, indicating low to moderate levels of G×E with some re-ranking of breeding values across environments. However, substantial G×E was evident for survival where only a moderate genetic correlation (rg = 0.58 ± 0.07) was observed for survival across different environments. A re-ranking of breeding values is likely for this trait and genetic improvement of shrimp for survival in one environment may not be effective in other environments. The results from ANOVA-based studies show that G×E accounted for 6.42 ± 1.05% and 7.13 ± 3.46% of the variation for growth and survival traits, respectively. (4) Conclusion: The significance of G×E necessitates tailored genetic improvement programs in commercial shrimp breeding. We discuss the scope and challenges of G×E for shrimp breeding programs, including opportunities of implementing G×E in genomic selection programs. [ABSTRACT FROM AUTHOR]

The fastest-growing animal food-producing agricultural sector in the world is aquaculture, which accounts for almost half of the world's food fish production. However, the expansion of high-density fish populations also brings forth a challenge-the rapid transmission and spread of infectious agents including several viral, bacterial, and parasitic diseases. Expansion and successful development of sustainable aquaculture practice and increasing production largely depends upon the prevention and control of outbreaks of several emerging and re-emerging infectious diseases which can result in economic loss, food safety hazards, and environmental hazards. Vaccination strategies have become highly effective and economical in protecting the health of fish and other aquaculture organisms from various infectious diseases such as edwardsiellosis, motile aeromonas septicemia (MAS), Tilapia Lake Virus (TiLV) disease, infectious salmon anemia (ISA), vibriosis, and white spot disease etc. An ideal vaccine is expected to be safe, effective, economical, and easily administered. Most of the available fish vaccines are empirically designed vaccines based on inactivated or live attenuated bacterin vaccines. Novel advances in the fields of immunology, biotechnology, and molecular biology have led to the development in designing novel and effective fish vaccines and the improvement of the existing vaccines to provide sufficient immune protection against diseases. This review investigates the currently available fish vaccines for use in finfish aquaculture against different infectious diseases different mode of vaccine administration with addressing pros and cons in detail. The information was collected from different secondary sources, and then compiled systematically. [ABSTRACT FROM AUTHOR]

In recent years, nanobubbles have become a focal point of academic research. Unlike macroscopic bubbles, nanobubbles possess unique properties that have led to their widespread application in various fields such as medicine, aquaculture, and mineral flotation. Particularly, the ultra-high stability of nanobubbles, which can persist for hours or even months, has become a hot topic. However, a systematic summary of the development history of nanobubbles is still lacking. Addressing this gap, this paper outlines the development history of nanobubbles and discusses the research progress on their stability. The paper also highlights the applicability and limitations of existing theories and summarizes the impact of nanobubble stability on flotation processes. This study aims to expand the potential research value of nanobubbles and provide new directions for further studies on their stability. [ABSTRACT FROM AUTHOR]

Maintaining genetic diversity in cultured shellfish can be challenging due to high variance in individual reproductive success, founder effects, and rapid genetic drift, but is important to retain adaptive potential and avoid inbreeding depression. To support broodstock management and selective breeding in cultured Pacific oysters (Crassostrea (Magallana) gigas), we developed an amplicon panel targeting 592 genomic regions and SNP variants with an average of 50 amplicons per chromosome. Target SNPs were selected based on elevated observed heterozygosity or differentiation in Pacific oyster populations in British Columbia, Canada. The use of the panel for parentage applications was evaluated using multiple generations of oysters from a breeding program on Vancouver Island, Canada (n = 181) and families selected for Ostreid herpesvirus-1 resistance from the Molluscan Broodstock Program in Oregon, USA (n = 136). Population characterization was evaluated using wild, naturalized, farmed, or hatchery oysters sampled throughout the Northern Hemisphere (n = 189). Technical replicates showed high genotype concordance (97.5%; n = 68 replicates). Parentage analysis found suspected pedigree and sample handling errors, demonstrating the panel's value for quality control in breeding programs. Suspected null alleles were identified and found to be largely population dependent, suggesting population-specific variation impacting target amplification. Null alleles were identified using existing data without the need for pedigree information, and once they were removed, assignment rates increased to 93.0 and 86.0% of possible assignments in the two breeding program datasets. A pipeline for analyzing the amplicon sequence data from sequencer output, amplitools , is also provided. [ABSTRACT FROM AUTHOR]

Aquaculture, commonly conceived as "fish farming," includes the culture of anininls, plants, or other species in water. Although about 70% of Earth's surfnce is covered by water, aquaculture often uses much snialler spaces such as tanks, ponds, mceways, or aquatic enclosures to grow aquatic food, fiber, mid other resources. Theologically, humans are called to "protect and serve" (Gen. 2:15), and throughout the Bible, there nre calls to good stezoardship and cultivation while allowing for fruitfulness of other creatures. Biblically, fish are seen as God-created aquatic creatures, often used as food, with implications for wise stewardship (e.g., Psalm 8). At the present, many fisheries around the world are overfished. Sustainable nqunculture should address environmental, economic, and health concerns, and it could help reduce the stress on natural fisheries. As the fastest-growing protein sector, aquaculture now produces more seafood than the wild / mrvest of all the world 's oceans (now approximately 120 niillion metric tons per year). This promising and expanding field (approximately 6-8% growth per year over the last 50 years) includes extremely efficient converters of protein, micro- and nincro-algae (seaweeds) that con absorb unwanted wastes and clean the water, and filter feeders such as oysters and clams that Clear the water of algne and other particles, simultaneously contributing various eco-system services and habitat. Ongoing problems include pathogenic and related disease issues. environmental pollution in sulface waters, food st#ty, increasing automation utilization, potential genetic concerns, and the relatively recent start of modern aqua-culture (most nqunculture growth has occurred since 1970). This article addresses each of these hurdles, identifies areas of theological and ethical concern, and clarifies matters of interest to Christians and others, suggesting possible ways forzoard in this fast-growing but cliallenging field. [ABSTRACT FROM AUTHOR]

Aquaculture, the world's fastest-growing food production sector, is critical for addressing food security concerns because of its potential to deliver high-quality, nutrient-rich supplies by 2050. This review assesses the effectiveness of CRISPR/Cas9 genome editing technology in enhancing desirable traits in fish species, including growth rates, muscle quality, disease resistance, pigmentation, and more. It also focuses on the potential effectiveness of the technology in allowing precise and targeted modifications of fish DNA to improve desirable characteristics. Many studies have reported successful applications of CRISPR/Cas9, such as knocking out reproductive genes to control reproduction and sex determination, enhancing feed conversion efficiency, and reducing off-target effects. Additionally, this technology has contributed to environmental sustainability by reducing nitrogen-rich waste and improving the nutritional composition of fish. However, the acceptance of CRISPR/Cas9 modified fish by the public and consumers is hindered by concerns regarding public perception, potential ecological impacts, and regulatory frameworks. To gain public approval and consumer confidence, clear communication about the editing process, as well as data on the safety and environmental considerations of genetically modified fish, are essential. This review paper discusses these challenges, provides possible solutions, and recommends future research on the integration of CRISPR/Cas9 into sustainable aquaculture practices, focusing on the responsible management of genetically modified fish to enable the creation of growth and disease-resistant strains. In conclusion, this review highlights the transformative potential of CRISPR/Cas9 technology in improving fish traits, while also considering the challenges and ethical considerations associated with sustainable and responsible practices in aquaculture. [ABSTRACT FROM AUTHOR]

Artificial Intelligence (AI) and Machine Learning (ML) can assist producers to better manage recirculating aquaculture systems (RASs). ML is a data-intensive process, and model performance primarily depends on the quality of training data. Relatively higher fish density and water turbidity in intensive RAS culture produce major challenges in acquiring high-quality underwater image data. Additionally, the manual image annotation involved in model training can be subjective, time-consuming, and labor-intensive. Therefore, the presented study aimed to simulate fish schooling behavior for RAS conditions and investigate the feasibility of using computer-simulated virtual images to train a robust fish detection model. Additionally, to expedite the model training and automate the virtual image annotation, a process flow was developed. The 'virtual model' performances were compared with models trained on real-world images and combinations of real and virtual images. The results of the study indicate that the virtual model trained solely with computer-simulated images could not perform satisfactorily (mAP = 62.8%, F1 score = 0.61) to detect fish in a real RAS environment; however, replacing a small number of the virtual images with real images in the training dataset significantly improved the model's performance. The M6 mixed model trained with 630 virtual and 70 real images (virtual-to-real image ratio: 90:10) achieved mAP and F1 scores of 91.8% and 0.87, respectively. Furthermore, the training time cost for the M6 model was seven times shorter than that for the 'real model'. Overall, the virtual simulation approach exhibited great promise in rapidly training a reliable fish detection model for RAS operations. [ABSTRACT FROM AUTHOR]

Simple Summary: Natural feed attractants are regularly used in aquafeeds to improve their palatability but show a number of disadvantages related to their natural origin, such as inconsistent quality of the raw materials, variable availability, and their dependency from natural sources. Contrariwise, synthetic flavors obtained through standardized processes are emerging as novel, more sustainable and effective substitutes to natural ones. The aim of the present study was to evaluate the use of synthetic flavors as novel feed attractants in zebrafish (Danio rerio) diet. A multidisciplinary approach was used to evaluate the dietary effects of the tested flavors—two attractive and one repulsive—on fish feed intake and growth, as well as on appetite regulation, brain reward system, and reproductive performances. Both diets containing the attractive flavors enhanced zebrafish feed intake and growth, promoting the fish reproductive performances without affecting fish welfare. Having ingested more feed, fish were able to invest to a greater extent in high-metabolic-demanding processes such as growth and reproduction. Improving the feeding practices of aquaculture, especially obtaining a quick feeding response by the fish, is a critical aspect for aquafeed production to minimize both the economic and environmental impacts. The present study aimed to test over a six-month period different synthetic flavors in zebrafish (Danio rerio) as an experimental model. Specifically, two attractive and one repulsive synthetic flavors were added (1% w/w) to a specific zebrafish diet, which was administered to the fish during the whole life cycle (from larvae to adults), to evaluate their physiological responses, emphasizing fish welfare, feed intake, growth, reward mechanisms, and reproductive performances. Fish welfare was not affected by all tested flavors, while both attractive flavors promoted fish feed ingestion and growth. The results were supported by both molecular and immunohistochemical analyses on appetite-regulating neurohormonal signals, along with the influence of the feed hedonic properties induced by the brain reward sensation, as demonstrated by the dopamine receptor gene expression. Finally, the present study demonstrated that a higher feed intake also had positive implications on fish reproductive performances, suggesting a promising role of synthetic flavors for the aquaculture industry. In conclusion, the results highlighted the potential of synthetic flavors to improve fish feeding strategies by providing a consistent and effective alternative to traditional stimulants, thereby reducing dependence on natural sources. [ABSTRACT FROM AUTHOR]

Simple Summary: Aquaculture is presented as a viable solution to overfishing and the growing demand for marine products. This study investigates the rearing of rainbow trout (Oncorhynchus mykiss) in a recirculating system at 3000 m above sea level, in Copaquilla, northern Chile. A total of 5000 juvenile trout were transported from the Rio Blanco fish farm over 2100 km. For 20 months, growth parameters were evaluated, including specific growth rate, weight gain, feed conversion, survival, and Fulton's condition factor. The results indicated normal growth and good quality, confirming the feasibility of trout aquaculture at a high altitude. This suggests new opportunities for aquaculture in the Andean region of northern Chile. Given the overexploitation of fisheries and the growing consumption of sea products, aquaculture is emerging as an alternative to meet the demand for protein at regional, national, and global levels. In northern Chile, the foothills of the Andes offer an opportunity for sustainable economic diversification. In this study, results of a rainbow trout (Oncorhynchus mykiss) culture in a recirculation system are presented, analyzing its growth and performance under altitude conditions. The research was carried out in Copaquilla, a small area in the foothills of northern Chile, 3000 m above sea level. Five thousand 15 g juvenile trout were acquired and transported by land from the Rio Blanco fish farm, successfully traveling more than 2100 km. During the 20-month-long culture, several growth parameters were evaluated, including specific growth rate, percentage of weight growth, feed conversion factor, survival, and Fulton's condition factor. All these parameters were within the normal growth range for trout, evidencing good-quality growth of the organisms. The results offer evidence that rainbow trout farming at 3000 m above sea level is viable and presents a viable performance, opening new opportunities for aquaculture in northern Chile. [ABSTRACT FROM AUTHOR]

Simple Summary: Freshwater mussels create habitat, filter water, and enhance food webs, but they are also among the world's most imperiled taxa. Conservation efforts largely rely on captive propagation in which mussels are grown in protected aquaculture environments (hatcheries) for later release. Recent evidence has highlighted the importance of pathogens in population losses of freshwater mussels. In response to ongoing mass mortality events of freshwater mussels in the Upper Tennessee River Basin in Virginia and Tennessee, USA, we conducted a multi-year study to document viruses across multiple restoration sites and compare them to viruses in mussels from the hatchery. Viral communities changed greatly after mussels were released. Of the 681 viruses of the 27 families we documented, only 20 viruses were found exclusively in hatchery mussels, compared to 451 viruses found only in mussels stocked to the wild. After release, mussels rapidly acquired new viruses, and the number of viruses increased steadily over time. These findings have implications for how mussel introduction programs might be managed for greater success, for example, by incorporating acclimatization periods prior to full release. Freshwater mussels (order: Unionida) are highly imperiled globally and are increasingly the focus of captive propagation efforts to protect and restore wild populations. The Upper Tennessee River Basin (UTRB) in Virginia is a freshwater biodiversity hotspot hosting at least 45 of North America's ~300 species of freshwater mussels, including 21 threatened and endangered species listed under the U.S. Endangered Species Act. Recent studies have documented that viruses and other microbes have contributed to freshwater mussel population declines in the UTRB. We conducted a multi-year longitudinal study of captive-reared hatchery mussels released to restoration sites throughout the UTRB to evaluate their viromes and compare them to captive hatchery environments. We documented 681 viruses from 27 families. The hatchery mussels had significantly less viruses than those deployed to wild sites, with only 20 viruses unique to the hatchery mussels. After the hatchery mussels were released into the wild, their number of viruses initially spiked and then increased steadily over time, with 451 viruses in total unique to the mussels in the wild. We found Clinch densovirus 1 (CDNV-1), a virus previously associated with mass mortality events in the Clinch River, in all samples, but the wild site mussels consistently had significantly higher CDNV-1 levels than those held in the hatchery. Our data document substantial differences between the viruses in the mussels in the hatchery and wild environments and rapid virome shifts after the mussels are released to the wild sites. These findings indicate that mussel release programs might benefit from acclimatization periods or other measures to mitigate the potential negative effects of rapid exposure to infectious agents found in natural environments. [ABSTRACT FROM AUTHOR]

Antibiotics can be introduced from aquaculture facilities into marine sediments, where they may affect benthic communities and generate antimicrobial resistance (AMR). Antibiotics can be used in tandem with antiparasitics, and the effects of their combined usage could affect AMR patterns and transmit antibiotic resistance genes. This study compiles patterns of antibiotic and antiparasitic drug usage from 2016 to 2021 in British Columbia, New Brunswick, Newfoundland and Labrador, and Nova Scotia (Canada) and compares them internationally. There is an overall reduction in rates of antibiotic use per fish produced in Canadian aquaculture sites from 2016 to 2021. Compared to other salmon-producing countries, Canadian antibiotic rates per tonne of fish are lower than in Chile, but higher, on average, than in Norway and Scotland. Florfenicol (FLO) and oxytetracycline (OTC) are the prevalent antibiotics used in most salmon-producing countries, including Canada. We also note that <30% of sites used two drugs per year and <18% of sites used three drugs, with most of these sites using one antibiotic and one antiparasitic. There is an increase in FLO and a decrease use in OTC use in Canada, which could be positive environmentally considering FLO's lower dose and environmental persistence. The east and west coasts show comparable antibiotic rates, with an average rate of 78 mg/kg in BC and 76 mg/kg in the Atlantic provinces; however, they have differences in the type of antibiotic (average use of OTC: BC 38 mg/kg vs. Atlantic 214 mg/kg; average use of FLO: BC 40 mg/kg vs. Atlantic 10 mg/kg). Other distinctions between coasts include variation in the timing of treatments, with seasonal use in the Atlantic provinces (May–November) and year-round use in BC. This pattern is likely influenced by differences in water temperature and, subsequently, potential susceptibility to aquatic diseases. Disease prevalence and veterinarian choice/preferred treatments are factors that, we hypothesize, may impact treatment choice; however, additional information is required to adequately comment on this point. This data compilation includes spatial descriptions that could be used for area prioritization in future studies. [ABSTRACT FROM AUTHOR]

Some hosts harbor more parasites than others. Overdispersion of parasitism suggests that coevolution with parasites may be more important to the biology and ecology of certain species. We examined patterns of parasitism and host traits in fished decapod crustaceans, which are economically and ecologically important worldwide. Using a synthesis approach, we determine that host life history, including habitat, longevity, sociality, invasion history, and fisheries involvement, correlate with the number and type of parasite species harbored. Indicator species analysis revealed close relationships between decapods and certain parasite groups, including crabs with rhizocephalans and dinoflagellates; crayfish with mesomycetozoans, oomycetes, branchiobdellids, and fungi; lobsters with copepods and amoebae; and shrimp with viruses. In contrast, Nematomorpha and Nemertea appear to be under-represented and under-studied as parasite groups in decapods. Decapods that are commercially fished, aquacultured, introduced outside their native range, and/or exhibit parental care tend to have higher parasite species richness (PSR). Parasite richness also increases with how well-studied a host group is, which we addressed with a machine learning algorithm that predicts false negative associations. Geographic range is commonly positively correlated with parasite richness, however reliable ranges are not available for most decapod species, highlighting a significant future research need. Identifying patterns such as these increases our broad understanding of decapod disease ecology but also enabled us to develop a series of recommendations on how to focus future research, management, and aquaculture development efforts. [ABSTRACT FROM AUTHOR]

A self-exchange aquaculture vessel stands as an environmentally sustainable solution for fish farming, capitalising on seawater utilization and minimising the risk of fish escapes through the implementation of perforated culture tanks. This research aims to lay the groundwork for the conceptual design, modelling, and simulation analysis of such vessels, focusing on how near-bottom perforation placement affects flow field characteristics within the culture tank. This paper presents a computational study using Computational Fluid Dynamics (CFD) to analyse self-exchange aquaculture vessels under both head and beam current conditions. The solution of conservation equations governing tank hydrodynamics is achieved using an implicit unsteady second-order Eulerian (finite volume) technique on optimised trimmed meshes. Experimental and predicted values for the vessel model's total resistance were evaluated using uncertainty analysis, validating the numerical model. It was found that proper positioning of perforations near the bottom significantly enhances the synergistic effect of fluid within the culture tank and the mixing characteristics of the flow field. To enhance water circulation, it is recommended to install two or more rows of perforations on the sides of self-exchange aquaculture vessels. The coordination between perforation placement and vessel structure should be considered to determine the optimal layout. By offering valuable insights into the effects of perforation placement, this study contributes to the development of more efficient and environmentally friendly aquaculture practices. • Hydrodynamics of a tank on the self-exchange aquaculture vessel studied by numerical methods. • The jet-like effect in the perforated culture tank was explored. • The influence mechanism of perforation placement on tank hydrodynamics was revealed. • Well-placed perforations boost fluid linkage inside and outside the tank. [ABSTRACT FROM AUTHOR]

The effects of different dietary lipid levels on lipid accumulation, inflammatory response, serum bio-chemical index and histological features of intestine and hepatopancreas of O. macrolepis was experimentally evaluated in an eight-weeks study. Fish (initial weight 50.11 ± 2.86 g) were fed with five iso-nitrogenous diets (around 390 g/kg protein) varying with lipid level (5%, 7%, 9%, 11%, 13%, being LL5, LL7, LL9, LL11, and LL13 respectively) in triplicates. Results showed that the content of crude lipid in carcass and hepatopancreas were not affected by dietary lipid levels (P > 0.05). Serum ALT, TP, HDL-c and MDA, etc., were not significantly affected by diets (P > 0.05), while serum total antioxidant capacity in LL9 and LL11 groups were significantly higher than the other groups (P < 0.05). Histological features of hepatopancreas and intestine showed no significant difference among the five diets (P > 0.05), while the height of intestine villus showed the higher trend in LL9 compared with other groups. The relative expression of lipid metabolism related genes (ppar α, cpt-1α, fas, and hsl) and immune response related genes (tlr 22, nrf 2, tnfα, and il-γ) in the hepatopancreas of fish fed diets differing in their crude lipid levels were not significantly different (P > 0.05). The results suggested that a proper dietary lipid level of 9%--11% could maintain higher antioxidant and health status of adult O.macrolepis. [ABSTRACT FROM AUTHOR]

Protein deficiency is a major difficulty for human needs in the past few decades, while different types of aquatic species are rich in high quality protein. Therefore, aquaculture is considered as the main developing food production sector globally. Bacterial infections are the main problem for aquaculture, and their outbreaks have a great impact on productivity, and previously indiscriminate use of antibiotics to control them. However, the emergence of multidrug-resistant pathogens might lead to sudden infectious disease outbreaks resulting in serious economic loss. Immunostimulants application is an effective technique to protect and enhance the immune system of aquatic animals and therefore improve aquaculture production. Nutrient immunostimulants such as essential fatty acids, amino acids, vitamins, and minerals are the most important responsibility to improve aquaculture production, as well as the cost of this method, which is effective, non-toxic, and environment friendly. These nutrient immunostimulants are supportive to increase the immune system, antioxidant, antiinflammatory, and infection resistance of aquatic animals. In addition, nutritional feed additives improved feed palatability and the excellence of aquatic products and also enhance gut functions. Some information is available on nutrient immunostimulants in aquaculture applications, and this review provides information on different kinds of nutritional administration used in aquaculture to enhance positive impacts on aquatic animals' health as well as feed quality development. This review will provide theoretical references for the application of nutrient immunostimulants in aquatic feeds. [ABSTRACT FROM AUTHOR]

Tetrodotoxin (TTX) is a potent neurotoxin firstly discovered in the ovary of pufferfish. The genetic basis of voltage-gated sodium channel resistance to TTX has been widely studied, but it remains unclear in the evolution history of voltage-gated sodium channel resistance to TTX in pufferfish with different TTX concentrations. In this study, six scn4aa coding sequences of pufferfish were firstly cloned and sequenced, then used to investigate the adaptive evolution of scn4aa associated with TTX concentration and reconstruct ancestral sequences with seven scn4aa of other fishes. The result of CODEML (codon substitution model) program from the PAML (phylogenetic analysis by maximum likelihood) package shows only in the genus of Takifugu, which contains TTX highly in the liver, under positive selection. The result also indicates that three of four positively selected sites are located in the intracellular regions, which may compensate for normal function. The ancestral sequence reconstruction may suggest that the replacements providing weak toxin resistance might have appeared first in scn4aa, then the genus Takifugu evolved stronger resistance to TTX later. These results contribute to the explanation of the evolutional history of voltage-gated sodium channel resistance to TTX in pufferfish. [ABSTRACT FROM AUTHOR]

The orange-spotted grouper (Epinephelus coioides) is one of the most popular fish in aquaculture due to its high economic value. However, the random occurrence of slow-growing population has led to profit loss. Therefore, transcriptomic analysis was performed to identify the molecular mechanisms involved in driving slow and fast growth, towards addressing this issue. Results show that low expression of developmental genes such as bone morphogenetic protein 1 (BMP 1) and somatostatin (SST), as well as GH/IGF axis-related genes (IGF-1, IGF2, IGFBP1, IGFBP2) and its downstream signalling pathways are correlated with the metabolism and digestive-system-related pathways in slow-growing groupers. Enrichment analysis further revealed that cellular process and biological regulation, as well as several pathways including glycolysis/gluconeogenesis, linoleic acid metabolism, protein digestion and absorption, and cholesterol metabolism were differentially expressed in slow-growing groups. The findings implied that the genomic architecture of growth between the different families raised and the likely problem of inbreeding have led to significantly reduced growth. It is also suggested that better husbandry practices (optimal dietary requirement) is also vital in supporting the success of a genetic improvement programme. These findings recommend growth improvement of orange-spotted groupers via molecular monitoring and artificial selection, complemented by specific dietary feeding. [ABSTRACT FROM AUTHOR]

Aquaculture has emerged as a pivotal alternative for seafood production, addressing the challenges posed by depleting wild fish stocks. However, the industry faces substantial challenges, notably the presence of harmful algal blooms (HABs), which can cause serious economic consequences. The proliferation of major species of phytoflagellates leading to massive fish mortality is on the rise, possibly attributed to increasing global temperatures and eutrophication. This underscores the critical need for robust detection, prevention, and mitigation strategies to safeguard the aquaculture industry. This contribution offers valuable insights into comprehending and addressing the challenges posed by HABs in the fish aquaculture sector in an era of global change. [ABSTRACT FROM AUTHOR]

The upsurge in the usage of plastics on a global scale has led to the widespread occurrence of microplastics (MPs) in aquatic environments. This review starts by outlining the current global scenario of plastic production. It then delves into the various sources of plastics and their entry into aquatic systems, including their impact on fisheries and the aquaculture sector. The detailed analysis of plastic entry and various methods of degradation into small sized, MPs followed by their transport, uptake and trophic transference have been reviewed systematically. Besides, the review summarizes the current knowledge on impacts of MPs, their additives and associated contaminants on organisms living in the aquatic environment, particularly fish thriving in cultured conditions. The analysis of effects of MPs alone and in association with other contaminants like heavy metals, organic pollutants and leachates reveal serious effects such as cytotoxicity, immune response, oxidative stress, neurotoxicity, barrier attributes and genotoxicity among various aquatic species particularly, fish. In addition, the present review discusses MPs invasion into aquaculture systems, explores risk assessment both in terms of exposure risk and toxicological risks associated with biochemical nature, size, shape and concentration of MPs. Besides, risk management strategies and future prospective to control MPs associated hazards have also been highlighted. In summary, the present review outlines the various sources, fate, impact of MPs in aquatic environment highlighting the need to recognize MPs pollution as a serious threat to aquatic organisms and to formulate strategies including prevention, reduce, reuse and safe disposal of plastic material. [ABSTRACT FROM AUTHOR]

Health of farmed fish and shellfish deserves a top priority. To help prevent and fight the global disease crisis in aquaculture, it is high time to further explore immunology. And high time for immunologists specialized in aquaculture to start taking aquaculture more seriously. As a first step forward, find here some challenges and many opportunities. [ABSTRACT FROM AUTHOR]

High‐intensity aquaculture may cause the aquatic animals to experience elevated stress levels, which in turn will increase their susceptibility to illness and result in substantial losses. Farmers frequently use chemical and antibiotic treatments for fish diseases, which have led to drug‐resistant and hazardous residues in farmed fish and the environment. Consequently, finding substitutes for chemicals and antibiotics is crucial for disease management in aquaculture. Terminalia catappa L. or commonly called Indian almond is well known for its medicinal properties and widely distributed in tropical and subtropical regions. T. catappa contains chemical compounds such as tannin and flavonoid that play an important role in antibacterial, anti‐allergy, anti‐inflammation, and anti‐cancer. Numerous studies have been conducted on leaves, bark, fruits, and seed of T. catappa for their applications, including medicinal, bioremediation (dye absorption), and aquaculture use. Much research can be found on the utilization of T. catappa leaves to improve fish well‐being, mostly in the ornamental fish industry. Extract of T. catappa has been found to improve fish survival, growth rate, inhibit bacterial infection, improve water quality, enhance fish colouration, and boost the immune response. Although T. catappa leaves have undergone substantial research, it has not been systematically described in connection to fish species used in aquaculture. This review will systematically discuss the biotoxicity of T. catappa evaluation of growth‐promoting properties, eggs and larviculture performance, and enhancement of fish resistance to various bacteria, fungi, and parasites. In addition, the article also highlights the future perspectives of using T. catappa to improve fish culture. [ABSTRACT FROM AUTHOR]

The effective implementation of machine vision has played a crucial role in advancing intelligent aquaculture across various domains. Stereo vision, as a branch of machine vision, has become a mainstream technology in aquaculture. Its distinctive capability to conduct comprehensive underwater monitoring from multiple angles, unaffected by object occlusion has propelled it to the forefront of aquaculture applications. This article offers a comprehensive review of the diverse applications of stereo vision in aquaculture spanning from its inception to present. The exploration encompasses its role in crucial areas such as biomass estimation and behavioural analysis, which include fish counting, weight estimation, swimming behaviour, feeding behaviour and abnormal behaviour. Furthermore, the paper delves into the advantages of stereo vision over traditional 2D machine vision approaches, while also acknowledging limitations, and identifying future challenges that must be addressed to fully leverage its potential in aquaculture. The review emphasizes the prospect of advancement in deep learning stereo‐matching algorithms specifically designed for underwater environments to catalyse a breakthrough in stereo vision technology. In summary, this review aims to provide researchers and practitioners with a better understanding of the current development of stereo vision in aquaculture, optimizing stereo vision technology and better serving the aquaculture field. [ABSTRACT FROM AUTHOR]

Mass mortality events (MMEs) are defined as the death of large numbers of fish over a short period of time. These events can result in catastrophic losses to the Atlantic salmon aquaculture industry and the local economy. However, they are challenging to understand because of their relative infrequency and the high number of potential factors involved. As a result, the causes and consequences of MMEs in Atlantic salmon aquaculture are not well understood. In this study, we developed a structural network of causal risk factors for MMEs for aquaculture and the communities that depend on Atlantic salmon aquaculture. Using the Interpretive Structural Modeling (ISM) technique, we analysed the causes of Atlantic salmon mass mortalities due to environmental (abiotic), biological (biotic) and nutritional risk factors. The consequences of MMEs were also assessed for the occupational health and safety of aquaculture workers and their implications for the livelihoods of local communities. This structural network deepens our understanding of MMEs and points to management actions and interventions that can help mitigate mass mortalities. MMEs are typically not the result of a single risk factor but are caused by the systematic interaction of risk factors related to the environment, fish diseases, feeding/nutrition and cage‐site management. Results also indicate that considerations of health and safety risk, through pre‐ and post‐event risk assessments, may help to minimize workplace injuries and eliminate potential risks of human fatalities. Company and government‐assisted socio‐economic measures could help mitigate post‐mass mortality impacts. Appropriate and timely management actions may help reduce MMEs at Atlantic salmon cage sites and minimize the physical and social vulnerabilities of workers and local communities. [ABSTRACT FROM AUTHOR]

Kappaphycus alvarezii is a superior product of Indonesian fisheries, producing carrageenan which is used as a food, cosmetic, and medicinal ingredient. K. striatus is a carrageenan-producing red alga that began to be cultivated in the Ekas waters of East Lombok in 2023. K. striatus is more resistant to epiphytes and sudden climate change. The purpose of this study was to analyze the growth of K. alvarezii and K. striatus. The research was conducted in Ekas Bay, Jerowaru District, East Lombok Regency. The research was conducted from November 8, 2023, to January 8, 2024. This research used an experimental method with T-test analysis. Treatments in this research are the cultivation of K. alvarezii and K. striatus. Cultivation was carried out at Ekas Bay. Analysis of seaweed carrageenan was carried out in the Laboratory of Fish Production and Reproduction, Aquaculture Study Program, Faculty of Agriculture, Mataram University. The result of this research is K. striatus has a higher absolute weight of 154 g compared to K. alvarezii seaweed which is 72 g. The specific growth rate of K. striatus higher at 2,98 % / day compared to K. alvarezii is 1, 98 %/day. The yield of carrageenan of K. alvarezii is 1,95% and K. striatus 0,88%. Antioxidant of K. alvarezii is 5,46 % and K. striatus is 7,42%. Chlorophyll K. alvarezii is 5,77 mg/l and K. striatus is 3,51 mg/l. The conclusion of this study is the growth of K. striatus faster than K. alvarezii. [ABSTRACT FROM AUTHOR]

The understanding of the increasing population density of copepods in culture tanks contributes to establish their stable cultures at high productions for aquaculture industry, which are preferred live feeds for fish larvae. In the present study, the semibenthic copepod Pseudodiaptomus nihonkaiensis were used as target species. This genus is often found from brackish water and its tolerance to a wide range of salinity and water quality has attracted attention as a potential commercial live feed for aquaculture. Population growth and egg production in the semi-benthic copepod P. nihonkaiensis are investigated. Copepods were cultured in tanks without mesh, with mesh lining tank walls, and with 2, 4 or 8 mesh partitions in each tank to identify the effect of substratum surface area on copepod population density. Linear regression revealed peaks in nauplius, copepodite, adult and total densities, and the cumulative number of eggs for all sampling events to correlate positively with substratum surface area (p<0.01). Greater substratum area within a culture tank may improve egg production by increasing habitat for ovigerous females. The simple installation of inexpensive artificial substrata in existing copepod culture tanks might improve the feasibility of using copepods as a live food in commercial-scale aquaculture [ABSTRACT FROM AUTHOR]

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Increasing need for food resources, State of Indonesia to strive to maximize the output of food production. Not only in agriculture but also aquaculture results are also trying to be improved. This is also supported by the increase of Indonesia’s national fish consumption rate from 50.69 Kg per capita in 2018 to 55.37 Kg per capita in 2021. Recent aquaculture research only explored topics about monitoring the cultivation environment. But there have been no studies exploring how bad the impact of weather on the process of farming. Hence, this study aims to measure the influence of weather on freshwater aquaculture pond water quality and analyze its impact on fish growth namely Oreochromis Sp., using pH sensors and dissolved oxygen (DO). Then a weather simulation was carried out based on Indonesia’s tropical climate, which majorly consists of sunny and rainy weather. The experimental results indicate the instability of the pH value during the rainy period. DO values tend to decrease at the end of periods of sunny weather. Moreover, fish growth analysis showed that there was a decrease in food conversion ratio (FCR) by 0.956, specific growth rate (SGR) by 2.13% and survival rate (SR) by 5.715% during rainy weather. [ABSTRACT FROM AUTHOR]

Uganda's aquaculture sub-sector is rapidly developing, contributing up to 22% of all fish produced in the country. However, this sub-sector still faces challenges related to limited access to extension services, production inputs, post-harvest handling facilities, disease incidences, value-addition skills, and weak market linkages. Although there have been isolated interventions at different aquaculture value-chain nodes, the registered challenges continue to recur. To address prioritized issues in Uganda's aquaculture sub-sector, nine zonal aquaculture innovation platforms were established based on the country's nine agro-ecological zones. These platforms brought together different stakeholders to identify solutions to common problems in each aquaculture value-chain node. Each innovation platform consisted of fish farmers, hatchery operators, traders, extension workers, researchers, input dealers, local leaders, and financial institutions. During a series of zonal workshops, participants identified constraints on the aquaculture sub-sector in their respective zones. From the findings, there are technical knowledge gaps across the entire aquaculture value chain, mainly affecting hatchery operators, input suppliers, and farmers. Therefore, there is need for coordinated interventions to enhance access to production technologies, innovations, knowledge, and skill transfer to promote gender-centered aquaculture development. [ABSTRACT FROM AUTHOR]

Firms must increasingly grapple with complex sustainability challenges. Business model innovation is needed to achieve radical sustainability improvements. Recent research highlights the interrelatedness between business model innovation, dynamic capabilities, and organizational design, calling for empirical work to better illuminate these relationships. These connections are relevant in sustainability contexts, where organizational barriers and drivers have been shown to either facilitate or hinder the development of dynamic capabilities for sustainable business model innovation (SBMI). This paper presents a case study of an emergent low‐carbon offshore aquaculture value chain in Norway to illuminate the importance and roles of these organizational factors. Based on our analysis, we identify those barriers and drivers present in the Norwegian salmon aquaculture industry; add to the body of theory around organizational design, dynamic capabilities, and SBMI; and offer insights for practitioners in the aquaculture sector aiming to develop dynamic capabilities for SBMI. [ABSTRACT FROM AUTHOR]

Recycled Concrete Aggregate (RCA) of the chemical and biological effects must be understood to avoid potential adverse impacts to the bay's aquatic ecosystem. RCA application as a base material for oyster reefs did not adversely affect oyster spat growth and survival, or the surrounding environment. Evaluated RCA leaching for petroleum byproducts showed that RCA as a base material for oyster reefs did not leach any hydrocarbon chemicals, and no water extractable SVOC were detected. The research found potential RCA application to the Chesapeake Bay watershed as a bottom conditioning material for oyster aquaculture. Overall, the findings support the use of RCA for oyster aquaculture. [ABSTRACT FROM AUTHOR]

There is little information available on antibiotic resistance (ABR) within shrimp aquaculture environments. The aim of this study was to investigate the presence of antibiotic resistance genes (ARGs) in shrimp farming operations in Atacames, Ecuador. Water samples (n = 162) and shrimp samples (n = 54) were collected from three shrimp farming operations. Samples were cultured and a subset of isolates that grew in the presence of ceftriaxone, a third-generation cephalosporin, were analyzed using whole-genome sequencing (WGS). Among the sequenced isolates (n = 44), 73% of the isolates contained at least one ARG and the average number of ARGs per isolate was two, with a median of 3.5 ARGs. Antibiotic resistance genes that confer resistance to the β-lactam class of antibiotics were observed in 65% of the sequenced isolates from water (20/31) and 54% of the isolates from shrimp (7/13). We identified 61 different ARGs across the 44 sequenced isolates, which conferred resistance to nine antibiotic classes. Over half of all sequenced isolates (59%, n = 26) carried ARGs that confer resistance to more than one class of antibiotics. ARGs for certain antibiotic classes were more common, including beta-lactams (26 ARGs); aminoglycosides (11 ARGs); chloramphenicol (three ARGs); and trimethoprim (four ARGs). Sequenced isolates consisted of a diverse array of bacterial orders and species, including Escherichia coli (48%), Klebsiella pneumoniae (7%), Aeromonadales (7%), Pseudomonadales (16%), Enterobacter cloacae (2%), and Citrobacter freundii (2%). Many ARGs were shared across diverse species, underscoring the risk of horizontal gene transfer in these environments. This study indicated the widespread presence of extended-spectrum β-lactamase (ESBL) genes in shrimp aquaculture, including blaCTX-M, blaSHV, and blaTEM genes. Increased antibiotic resistance surveillance of shrimp farms and identification of aquaculture operation-level risk factors, such as antibiotic use, will likely be important for mitigating the spread of ARGs of clinical significance. [ABSTRACT FROM AUTHOR]

Flexible cages are widely used in marine aquaculture, yet their mechanical features in extreme seas are still unclear. This study proposes a numerical algorithm to solve the coupled response of the multiple cage systems. The net and mooring lines are modeled using the lumped-mass model, while the flexible floating collar system is assessed with the large-deformation FEM model, and the two models are coupled through an iterative scheme. Sea trials are conducted, and the motion of the cage is obtained using an image processing technique, which validates the numerical algorithm. Using the proposed numerical algorithm, a series of simulations are performed to investigate the response of flexible cages in extreme seas. Motions, line tensions, and structural sectional forces are studied, and the effects of factors such as the wavelength of incident waves and the diameter of collar pipes are investigated. [ABSTRACT FROM AUTHOR]

To investigate the influence of the chord length and frequency of an oscillating hydrofoil device on the discharge characteristics of floating particulate matter, in this study, we take raceway aquaculture as an example and systematically compare and analyze the flow field characteristics of the hydrofoil device with different chord lengths and frequencies, as well as the sewage discharge performance of the raceway based on Computational Fluid Dynamics (CFD). The results indicate that in the particulate matter discharge process of raceway aquaculture, when the chord length and motion frequency of the hydrofoil device are 0.1 W (W is the width of the raceway) and 1.0 Hz, respectively, the anti-Karman vortex streets produced by the hydrofoil device are less affected by the wall, the flow field is the most uniform, the particulate matter discharge performance is the best, and the final floating particulate matter discharge rate reaches up to 99.09%. Adjusting the chord length of the hydrofoil can effectively ameliorate flow field reflux issues, enhancing the uniformity and flow performance of the flow field. When the chord length is 0.1 W, the uniformity of the flow field is optimal. When the chord length is 0.2 W, the flow performance of the flow field is superior. Increasing the frequency enhances the flow performance of the flow field, with an average increase of 0.1 Hz in motion frequency leading to a 19.42% improvement in the average velocity at the outlet. Based on this, we recommend the use of a hydrofoil device with a chord length of 0.1 W and a motion frequency of 1.0 Hz in the raceway aquaculture system to achieve optimal particulate matter discharge performance, providing a theoretical basis and practical guidance for using hydrofoil devices to improve the efficiency of floating particulate matter treatment in raceway aquaculture environments. [ABSTRACT FROM AUTHOR]

The salmon industry's challenges with skin health and sea lice emphasize the necessity for fish-sensitive measures like functional nutrition to boost skin health and fish welfare. The present study investigated the efficacy of krill meal (KM) for skin mucosal health and sea lice in Atlantic salmon (170 g). Following an 8-week feeding period, in duplicate tanks, on test diets (8% KM, 12% KM, and the control group), fish underwent a 2-week sea lice challenge, reaching 350 g. The 8% KM diet group had thicker skin epithelium (72.3 µ) compared to the 12% KM (51.3 µ) and the control groups (43.8 µ) after 8 weeks. Additionally, skin mucosal health parameters—cell size (208 µ2), cell density (25.2%), and defense activity (1.19)—were significantly enhanced with 8% KM compared to the 12% KM (cell size: 162.3 µ2, cell density: 17%, defense activity: 1.04) and the control group (cell size: 173.5 µ2, cell density: 16.4%, defense activity: 0.93). Furthermore, fish fed with 8% KM significantly showed the lowest sea lice, along with reduced cell size while maintaining a high abundance of skin mucous cells, suggesting efficient turnover of the skin mucosal layer to remove sea lice effectively. This study highlights the potential of KM as part of a functional nutrition strategy to enhance skin mucosal health and mitigate sea lice challenges. [ABSTRACT FROM AUTHOR]

People living in coastal areas have the advantage of accessing a high level of ecosystem services, but are also exposed to the dangers inherent in living by the sea in an area prone to natural disasters. To achieve sustainable utilization of the high ecosystem services of coastal areas, establishment of an effective marine policy based on regional characteristics of human–nature interactions is essential. We compared the structure of perceived satisfaction for five components of human well-being ('Security', 'Basic material for a good life', 'Good social relations', 'Health', and 'Freedom of choice and action') derived from coastal ecosystem services among three sites in Japan (Wakasa, Sanriku, and Shima) that share the same natural and social backgrounds: highly dependent on fisheries industry, located in the same climate regime (temperate zone), and have similar topographical features (ria coast). Structural equation modeling using the results from questionnaire surveys showed that the three sites shared a common basic structure of the interactions among the five components of human well-being. However, the intensity of interactions among components differed among the three sites. Questionnaire surveys on anxiety about natural disasters and future access to marine ecosystem services were simultaneously conducted at the three sites. The site-specific anxieties were responsible for the difference in the interactions among the five components of human well-being. We propose a method that quantitatively evaluates both positive (satisfaction) and negative (anxiety) aspects of respondents' subjective assessment as a useful tool for understanding the diversity of human–nature interactions caused by the region-specific natural and social backgrounds of coastal areas. [ABSTRACT FROM AUTHOR]

Microalgae offer distinct advantages as a nutritional source for aquaculture and as a means of wastewater bioremediation. Studying the phycosphere bacteria and understanding their complex interactions is essential to optimizing high-quality biomass growth. This study aimed to isolate, characterize, and identify bacteria from the phycosphere of marine microalgae and to determine their potential to enhance growth, metabolism, and bioremediation capabilities of Chaetoceros calcitrans in stress nutrient-poor media simulating aquaculture wastewater enriched with nitrate, nitrite, or phosphorus. Bacterial characterization included tests for auxin and siderophore production, biofilm formation, amylase activity, phosphate solubilization, mobility, and antagonism evaluation. When Alteromonas macleodii, Bacillus cereus, and Marinobacter sp. were selected and then enriched (107 CFU/mL) in co-culture with C. calcitrans, growth levels significantly increased in four of six Synthetic Aquaculture Wastewater (SAW) media. Pigment levels were higher in five of six SAW media, and lipid levels were higher in SAW rich in nitrite (SAWni50) and phosphorus (SAWpho50). In addition, C. calcitrans with or without the bacterial consortium demonstrated excellent phosphorus bioremediation, achieving 67.6% average removal in SAWpho50. Nitrate and nitrite assimilation rates were approximately 10% in SAWna and SAWni50. This study marks the inaugural identification of these bacteria as microalga growth-promoting bacteria (MGPB) for enhancing growth and lipid and pigment production in C. calcitrans, and it also documents a maximum of 69.13% phosphorus removal. [ABSTRACT FROM AUTHOR]

In the latest years, the concentration levels of certain metals and metalloids in the sediments of the Galician Rías have shown an increasing trend (e.g., As, Zn, Cu, Pb, Hg). These areas are also characterized by their richness in nutrients and their great aquaculture or mariculture activity, with the presence of more than 3500 mussel rafts in the Rías Baixas. The inner areas of the Galician Rías are subjected to activities that resuspend the sediment such as high levels of maritime traffic and dredging or cleaning operations. It is likely that a transfer of these elements to the water column happens during the resuspension of sediments caused by natural events or anthropogenic activities. In this study, selected samples of surface sediments of the Ría de Pontevedra (NW Spain) were subjected to a procedure of aerobic oxidation to determine the concentration of some elements (Fe, Mn, Cu, Cr, Pb, Hg, and Zn) released from the sediment to the aqueous phase. The experiment was carried out within 5 days. Measurements of pH and total concentration were taken both in water and sediment samples. Furthermore, speciation of trace elements was carried out in the sediment samples. Trace element concentrations were lower in the sediments during aerobic oxidation, being released to the aqueous phase. From an environmental point of view, Cu was the only trace element released in quantities that may be toxic for the organisms in the area. This problem of sediment oxidation related to dredging activities or natural storm conditions should be considered in environmental impact studies and transferred to stakeholders. [ABSTRACT FROM AUTHOR]

Metal contamination is a crucial environmental issue because of its persistence and toxicity, which can have deleterious effects on aquatic ecosystems. Coastal sediments serve as reservoirs for metals. Aquaculture is an important human activity in coastal areas; however, the actual status of metal distribution in these areas has not been well characterized. We investigated the distribution and geochemical speciation of metals in the sediments of the Japanese aquaculture areas of Shidugawa and Furue Bays. Total metals were generally in the order Zn > Cu > Ni > Cd > Pb and occurred at high levels at the sites in the inner parts and near the fish cages in the sampling areas. Pearson's correlations and principal component analysis results suggested that the metals in these aquaculture areas might originate from several sources, such as fish feces, fish feeds, corrosion of antifouling material residues, and local anthropogenic activities, including fertilizers and manures. Furthermore, metals were mainly in silts and fine sediments, associated with residual speciation, followed by organic fractions, oxides, and high fractions of exchangeable Cd. These results provide important insights into the accumulation of metals in sediments for better managing marine aquaculture in Japan. [ABSTRACT FROM AUTHOR]

With the burgeoning global demand for seafood, potential solutions like aquaculture are increasingly significant, provided they address issues like pollution and food security challenges in a sustainable manner. However, significant obstacles such as disease outbreaks and inaccurate biomass estimation underscore the need for optimized solutions. This paper proposes "Fish-Sense", a deep learning-based pipeline inspired by the human visual system's ability to recognize and classify objects, developed in conjunction with fish farms, aiming to enhance disease detection and biomass estimation in the aquaculture industry. Our automated framework is two-pronged: one module for biomass estimation using deep learning algorithms to segment fish, classify species, and estimate biomass; and another for disease symptom detection symptoms, employing deep learning algorithms to classify fish into healthy and unhealthy categories, and subsequently identifying symptoms and locations of bacterial infections if a fish is classified as unhealthy. To overcome data scarcity in this field, we have created four novel real-world datasets for fish segmentation, health classification, species classification, and fish part segmentation. Our biomass estimation algorithms demonstrated substantial accuracy across five species, and the health classification. These algorithms provide a foundation for the development of industrial software solutions to improve fish health monitoring in aquaculture farms. Our integrated pipeline facilitates the transition from research to real-world applications, potentially encouraging responsible aquaculture practices. Nevertheless, these advancements must be seen as part of a comprehensive strategy aimed at improving the aquaculture industry's sustainability and efficiency, in line with the United Nations' Sustainable Development Goals' evolving interpretations. The code, trained models, and the data for this project can be obtained from the following GitHub repository: https://github.com/Vision-At-SEECS/Fish-Sense. [ABSTRACT FROM AUTHOR]

The study aimed to monitor the prevalence of ectoparasites and bacterial infestations in carp and to determine effective dosages of a new iodine-based treatment for disease prevention. The research found that carp were affected by ectoparasites G. kobayashii at 43.32%, L. cyprinacea at 46.67%, and P. lusiana at 31.71% in fish farming facilities within the Sumy Region. Treatment with the iodine-based product resulted in a reduction of infestation levels to 0.2%, including a decrease in the incidence of gyrodactylosis by 89.92-97.35%, lernaeosis by 79.13-95.91%, and philometroidosis by 81.30-91.16% compared to monitoring results. In investigations of bacterial infestations, it was found that the contamination of carp was higher for E. coli at the PC 'Shmatukha' by 8.0%, Aeromonas hydrophila by 47.97%, and Aeromonas salmonicida by 31.39%. Additionally, Pseudomonas anguilliseptica was detected at 19.48%, Streptococcus iniae at 13.27%, and Micrococcus spp. at 38.14% at LLC 'Ryasnyanske'. The presence of Staphylococcus aureus was recorded at 21.37%, and Listeria monocytogenes at 11.43% at LLC 'Bdzhola'; these figures were lower than those observed in other farms. Following treatment with the iodine-based agent, contamination of fish by E. coli decreased by 98.96% at the PC 'Shmatukha', 99.2% at LLC 'Bdzhola', and 84.32% at LLC 'Ryasnyanske'. Contamination of carp by A. hydrophila decreased by 94.51% at the PC 'Shmatukha', 80.28% at LLC 'Bdzhola', and 99.15% at LLC 'Ryasnyanske'. The presence of A. salmonicida was reduced by 97.23% at the PC 'Shmatukha', 94.00% at LLC 'Bdzhola', and 79.06% at LLC 'Ryasnyanske'. Contamination by S. aureus decreased by 92.15% at the PC 'Shmatukha', 98.74% at LLC 'Bdzhola', and 93.89% at LLC 'Ryasnyanske'. The levels of S. iniae decreased by 80.90% at the PC 'Shmatukha', 99.63% at LLC 'Bdzhola', and 83.30% at LLC 'Ryasnyanske'. Contamination by L. monocytogenes was reduced by 96.25% at the PC 'Shmatukha', 96.08% at LLC 'Bdzhola', and 98.13% at LLC 'Ryasnyanske'. Furthermore, Micrococcus spp. levels decreased by 93.74% at the PC 'Shmatukha', 96.37% at LLC 'Bdzhola', and 92.26% at LLC 'Ryasnyanske', in comparison to the monitoring results. The practical value of this study lies in controlling the spread of ectoparasites and developing preventive measures to eliminate ectoparasites and bacterial infestations in carp prior to their release into water bodies. [ABSTRACT FROM AUTHOR]

Seaweeds have been utilized for millennia in Asian countries, although they have only more recently become popular in Western society. They began to be used in ancient times because of their long-term properties and, nowadays, seaweeds are being targeted as a potential tool to combat climate change. There are not many laws governing seaweeds because they have just lately been utilized as food. However, guidelines are being developed to regulate their manufacture and use. Because of seaweed's tendency to accumulate components, whether helpful or poisonous, limited doses of certain substances have been established to prevent consumer overdosage. Aside from chemical safety, microbiological safety is important for people, and preventing any pathogen from spreading and infecting seaweeds is critical. As a result, systems and ways to safeguard consumers must be developed. Because various seaweed species have varied compositions, certain seaweeds may be safer nutraceuticals than others. To ensure the safety of seaweed-based food items, the HACCP (Hazard Analysis Critical Control Point) system needs to be used. The majority of seaweeds consumed come from aquaculture; however, others come from wild harvesting. To ensure the success of the cultures, the waters must be tested for chemicals and biological risks, as well as for the pH, salinity, and temperature. Seaweeds have enormous promise in many industries, but in the food industry, they are beginning to play a major role, and seizing the chances to produce innovative, safe, and sustainable food sources is strongly advised. This critical review investigates the real potential of seaweed as a human food source and as a nutraceutical solution. This review also focuses on the usage of seaweed as a food product and the procedures required to prepare it. In addition, it compiles information on the applicable legislation and regulations, and it addresses the lengthy road that has to be traveled to increase human well-being by employing a new food source in a controlled manner while simultaneously reducing the human population's health problems. [ABSTRACT FROM AUTHOR]

Aquaculture and waste valorization have the potential to show solid achievements toward food security and improvements in the circularity of resources, which are crucial aspects of achieving a sustainable lifestyle in agreeance with Agenda 2030 goals. This study aims to optimize and simplify the decision-making processes for the valorization of marine wastes (natural and from aquaculture) as secondary raw materials to produce high-value-added market goods. However, significant concentrations of pollutants may be present within wastes, compromising overall quality, and social dynamics can hinder their usage further. Goro's lagoon was chosen as a case study, where the relations between the ecosystem services, a thriving bivalve economy, and social dynamics are deeply rooted and intertwined. Therefore, in the manuscript cost–benefit and foresight analyses are conducted to determine the best usage for algal biomass considering pollution, social acceptance, and profitability. These analyses are virtually conducted on bio-refineries that could be operating in the case study's area: briefly, for a thirty-year running bio-plant, the CBA indicates the two best alternatives with an income of 5 billion euros (NPV, with a 5% discount rate) for a biofuel-only production facility, and a half for a multiproduct one, leading to the conclusion that the first is the best alternative. The foresight, instead, suggests a more cautious approach by considering external factors such as the environment and local inhabitants. Hence, the main innovation of this work consists of the decision-maker's holistic enlightenment toward the complexities and the hidden threats bound to this kind of closed-loop efficiency-boosting process, which eventually leads to optimized decision-making processes. [ABSTRACT FROM AUTHOR]

Plastics are a major source of marine pollution. One form of plastic pollution is microfibers, which are synthetic fibers five micrometers or smaller that are shed by artificial clothing. The size of microfibers enables them to easily be ingested by a number of marine organisms, including oysters. Oysters are filter feeders and a major aquaculture asset, which presents a concern for the effects of microfiber ingestion on human health. Very few studies have been conducted quantifying microfibers using Pacific oysters (Crassostrea gigas) sourced from California. This study quantifies microfiber content in the Pacific oyster farmed for human consumption in Morro Bay, California. Microfibers were quantified after being isolated from oyster samples. An average of 9.12 microfibers were recovered per oyster sample. Although some of the smaller oysters contained more microfibers compared to larger oysters, this difference was not significant. There also was no significant difference between the quantities of black and blue microfibers. However, there was a significant increase in quantities of black microfibers compared to green or red microfibers. The results of this study indicate that a large amount of microfibers are present in commercial oysters, but more research needs to be conducted to determine how this will impact human health. [ABSTRACT FROM AUTHOR]

Recirculating Aquaculture System (RAS) is introduced in aquaculture farming industry to reduce water resource utilization, efficient the energy and land uses, and also help minimalize the water exchange. This system enables utilization of unsuitable land and promotes a sustainable environment in aquaculture industry. Furthermore, this technology has been established and proved efficient in monitoring the aquatic animal condition subsequently helps in maintaining the water quality and help remove solid particle wastes from the aquaculture treatment. As today, RAS has been developed with more effective technologies such as the use of UV irradiation, solid capture, protein skimmer and also provided with highly techno bio-filtration set. Basically, this system was applied for broodstock maturation, nursery phase, and grow-out production. In this review article, we provide an overview of RAS between the clear water, probiotic, and biofloc technology, and the advantages of its combination. Even though RAS and biofloc is two different parallel system, the application of the probiotic and biofloc in the semi-RAS application system is intense to be investigated. The synergistic effect of RAS using this combination towards high yield aquaculture production will be highlighted in this review paper. Expectantly this review paper will generate awareness and useful information on the RAS application in the aquaculture system operation with help in maximize the impact to the aquaculture yield production.

Simvastatin (SIM), a commonly prescribed drug for lowering serum-lipid, has been detected in many aquatic environments. Exposure to high concentrations of SIM in rats and aquatic organisms resulted in toxicity to hepatocytes and negative effects on development. However, there is a paucity of studies on the effects of long-term exposure to low concentrations of SIM on aquaculture species. This study aimed to analyze the effects of environmentally relevant concentrations (0.1 μg/L and 1 μg/L) of SIM exposure for two months on developing Mozambique tilapias. Surprisingly, the growth of male tilapias was enhanced after a 2-month SIM exposure. The analysis of testis histology revealed that after the SIM exposure, the development of the testis was delayed, and sperm production was reduced. Sequencing of testis transcriptomes showed that the exposure to SIM altered the expression of many genes related to the development of testes and spermatogenesis. These data suggest that a 2-month exposure to environmentally relevant concentrations of increases the growth of male tilapias by inhibiting sexual development of males.

Aquaculture, while offering significant contributions to global food security, generates substantial amounts of industrial waste, posing environmental and economic challenges. However, this waste also presents untapped potential for innovation in gastronomy. This paper explores the emerging trend of utilizing aquaculture industrial wastes in culinary practices, aiming to reduce waste, promote sustainability, and create novel gastronomic experiences. Through a review of literature and case studies, we examine various methods for repurposing aquaculture waste, including upcycling into new food products, incorporation into culinary dishes, and extraction of valuable components. Additionally, we highlight successful initiatives that have integrated aquaculture waste into gastronomy, emphasizing the benefits of waste reduction, sustainable food practices, and culinary innovation. Challenges such as food safety, taste, and consumer acceptance are acknowledged, with strategies proposed for addressing these issues. Finally, we discuss future directions for research and development in this field, identifying opportunities for collaboration between the aquaculture industry and the gastronomy sector. By exploring the utilization of aquaculture industrial wastes in gastronomy, this paper contributes to a deeper understanding of sustainable food practices and culinary creativity in the context of aquaculture waste management.