1,029 results on '"Carvalho, Edgar M."'
Search Results
202. IL-17 and Regulatory Cytokines (IL-10 and IL-27) in L. braziliensis Infection
- Author
-
Novoa, Roberto, Bacellar, Olívia, Nascimento, Márcia, Cardoso, Thiago M., Ramasawmy, Rajendranath, Oliveira, Walker N., Schriefer, Albert, and Carvalho, Edgar M.
- Subjects
Male ,Reverse Transcriptase Polymerase Chain Reaction ,Tumor Necrosis Factor-alpha ,Gene Expression Profiling ,Interleukins ,Interleukin-17 ,Infant ,Leishmaniasis, Cutaneous ,Enzyme-Linked Immunosorbent Assay ,Article ,Leishmania braziliensis ,Interleukin-10 ,Interferon-gamma ,Child, Preschool ,Humans ,Female ,Child ,Asymptomatic Infections - Abstract
Cutaneous leishmaniasis (CL) is characterized by high production of pro-inflammatory cytokines and development of pathology. Individuals with subclinical L. braziliensis infection (SC) have a positive skin test to leishmania, but do not develop disease. We evaluated whether the downregulation of inflammatory response in SC is mediated by IL-10 and IL-27 and whether IL-17 is associated with control of infection. Participants include SC individuals, patients with CL and healthy subjects. Cytokines protein and mRNA were detected by ELISA and real-time PCR. IFN-γ and TNF-α levels were higher in CL than in SC group. The IL-10 levels and mRNA for IL-10 were similar in both SC and CL. mRNA for IL-27 was increased in cells from SC after stimulation with L. braziliensis antigen. There was a tendency for increased levels of IL-17 in SC compared to CL. The weak type 1 immune response observed in SC L. braziliensis infection is not because of the regulatory effects of IL-10 and IL-27. The control of Leishmania infection may be mediated by innate immune response with participation of IL-17. The results from this pilot study warrant further larger studies to investigate the potential contributions of IL-17 and IL-27 to the control of L. braziliensis infection.
- Published
- 2011
203. Consequences of the association between HTLV-1 and tuberculosis
- Author
-
de Lourdes Santana Bastos, Maria, primary, Neves, Yuri, additional, Carvalho, Natália, additional, Souza, Anselmo, additional, Neto, Abraão, additional, Siqueira, Isadora, additional, Santos, Silvane, additional, and Carvalho, Edgar M, additional
- Published
- 2015
- Full Text
- View/download PDF
204. The clinical spectrum of HTLV-1 infection
- Author
-
Tanajura, Davi, primary, Neto, Abraão, additional, Siqueira, Isadora, additional, Gusmão, Valéria, additional, Oliveira, Paulo, additional, Giozza, Silvana, additional, Castro, Neviton, additional, Carvalho, Natalia, additional, Santos, Silvane, additional, and Carvalho, Edgar M, additional
- Published
- 2015
- Full Text
- View/download PDF
205. Role of Urine Neutrophil Gelatinase-Associated Lipocalin in the Early Diagnosis of Amphotericin B-Induced Acute Kidney Injury
- Author
-
Rocha, Paulo Novis, primary, Macedo, Michael Nascimento, additional, Kobayashi, Carla Dinamérica, additional, Moreno, Lis, additional, Guimarães, Luiz Henrique Santos, additional, Machado, Paulo Roberto Lima, additional, Badaró, Roberto, additional, Carvalho, Edgar M., additional, and Glesby, Marshall Jay, additional
- Published
- 2015
- Full Text
- View/download PDF
206. IL-22 Protects against Tissue Damage during Cutaneous Leishmaniasis
- Author
-
Gimblet, Ciara, primary, Loesche, Michael A., additional, Carvalho, Lucas, additional, Carvalho, Edgar M., additional, Grice, Elizabeth A., additional, Artis, David, additional, and Scott, Phillip, additional
- Published
- 2015
- Full Text
- View/download PDF
207. Age Modifies the Immunologic Response and Clinical Presentation of American Tegumentary Leishmaniasis
- Author
-
Carvalho, Augusto M., primary, Amorim, Camila F., additional, Lago, Alexsandro S., additional, Barbosa, Juliana L. S., additional, and Carvalho, Edgar M., additional
- Published
- 2015
- Full Text
- View/download PDF
208. Wound healing genes and susceptibility to cutaneous leishmaniasis in Brazil: Role of COL1A1
- Author
-
Almeida, Lucas, primary, Oliveira, Joyce, additional, Guimarães, Luiz Henrique, additional, Carvalho, Edgar M., additional, Blackwell, Jenefer M., additional, and Castellucci, Léa, additional
- Published
- 2015
- Full Text
- View/download PDF
209. Protective and Pathological Functions of CD8 + T Cells in Leishmania braziliensis Infection
- Author
-
Cardoso, Thiago Marconi, primary, Machado, Álvaro, additional, Costa, Diego Luiz, additional, Carvalho, Lucas P., additional, Queiroz, Adriano, additional, Machado, Paulo, additional, Scott, Phillip, additional, Carvalho, Edgar M., additional, and Bacellar, Olívia, additional
- Published
- 2015
- Full Text
- View/download PDF
210. Genomic Profiling of Human Leishmania braziliensis Lesions Identifies Transcriptional Modules Associated with Cutaneous Immunopathology
- Author
-
Novais, Fernanda O., primary, Carvalho, Lucas P., additional, Passos, Sara, additional, Roos, David S., additional, Carvalho, Edgar M., additional, Scott, Phillip, additional, and Beiting, Daniel P., additional
- Published
- 2015
- Full Text
- View/download PDF
211. Functional Activity of Monocytes and Macrophages in HTLV-1 Infected Subjects
- Author
-
Amorim, Camila F., primary, Souza, Anselmo S., additional, Diniz, Angela G., additional, Carvalho, Natália B., additional, Santos, Silvane B., additional, and Carvalho, Edgar M., additional
- Published
- 2014
- Full Text
- View/download PDF
212. Matrix Metalloproteinase 9 Production by Monocytes is Enhanced by TNF and Participates in the Pathology of Human Cutaneous Leishmaniasis
- Author
-
Campos, Taís M., primary, Passos, Sara T., additional, Novais, Fernanda O., additional, Beiting, Daniel P., additional, Costa, Rúbia S., additional, Queiroz, Adriano, additional, Mosser, David, additional, Scott, Phillip, additional, Carvalho, Edgar M., additional, and Carvalho, Lucas P., additional
- Published
- 2014
- Full Text
- View/download PDF
213. Interleucinas proinflamatorias en pacientes con dolor neuropático sometidos a tratamiento con Tramadol
- Author
-
Kraychete, Durval Campos, Sakata, Rioko Kimiko [UNIFESP], Issy, Adriana Machado [UNIFESP], Bacellar, Olívia, Jesus, Rogério Santos, Carvalho, Edgar M, UFBA Anestesiologia, Universidade Federal de São Paulo (UNIFESP), UFBA Laboratório de Imunologia, and UFBA
- Subjects
PAIN, neuropathic ,ANALGESIC ,CITOCINAS/IL-6 ,ANALGÉSICO ,CYTOKINES ,DOR, neuropática/hérnia de disco ,DOR, neuropática ,ANALGÉSICO/tramadol ,CITOCINAS/IL-1 ,CITOCINAS/TNF-± ,CITOCINAS/sTNF-R ,DOR, neuropática/síndrome do túnel do carpo ,CITOCINAS - Abstract
JUSTIFICATIVA E OBJETIVOS: As citocinas pró-inflamatórias têm função importante na fisiopatologia das síndromes dolorosas neuropáticas. O objetivo desse estudo foi avaliar os níveis plasmáticos de citocinas pró-inflamatórias antes e após o tratamento com tramadol em pacientes com hérnia discal e síndrome do túnel do carpo e compará-los com indivíduos normais. MÉTODO: Investigou-se 38 pacientes com dor neuropática por hérnia discal ou síndrome do túnel do carpo. Todos os pacientes foram tratados com tramadol de liberação controlada (100 mg em 12h) durante 10 dias. Realizaram-se coletas de sangue venoso (5 mL), no período matutino, antes do tratamento e no 11º dia e as amostras foram armazenadas até análise (-70ºC). Foram utilizados testes enzimáticos ELISA para dosagem de citocinas plasmáticas (TNF-±, IL-1, IL-6) e receptores sTNF-R1, (R & D Systems). Realizou-se dosagem de citocinas em soro de 10 voluntários sadios. RESULTADOS: A concentração de TNF-± antes (5,8 ± 2,8 pg.mL-1) foi significativamente maior que após o tramadol (4,8 ± 2,1 pg.mL-1; p = 0,04, Teste Mann-Whitney). Não houve diferença significativa de IL-1², IL-6 e sTNF-R1 antes e após o tratamento. As concentrações plasmáticas de TNF-± (sadios: 1,4 ± 0,5; pacientes com dor: 5,8 ± 2,8 pg.mL-1; p = 0.01) e IL-6 (sadios: 1,2 ± 0,8; pacientes com dor: 3,5 ± 2,6 pg.mL-1; p = 0,01) foram significativamente maiores nos pacientes com dor neuropática que nos voluntários, Teste de Mann-Whitney. CONCLUSÕES: Nos pacientes com hérnia discal e síndrome do túnel do carpo as concentrações plasmáticas de TNF-± e IL-6 foram maiores que em voluntários sadios, não havendo diferença das concentrações de sTNF-R e IL-1². Houve redução da concentração plasmática de TNF-± após tratamento com tramadol (100 mg em 12h), mas não de IL-6, sTNF-R e IL-1². BACKGROUND AND METHODS: Proinflammatory cytokines play an important role in the pathophysiology of neuropathic pain syndromes. The objective of this study was to evaluate plasma levels of proinflammatory cytokines before and after treatment with tramadol in patients with herniated intervertebral disks and carpal tunnel syndrome, and to compare them with normal individuals. METHODS: Thirty-eight patients with neuropathic pain secondary to herniated intervertebral disks or carpal tunnel syndrome participated in this study. All patients were treated with controlled release tramadol (100 mg every 12 hours) for 10 days. Venous blood (5 mL) was collected in the morning, before treatment and on the 11th day, and stored (-70° C) until analysis. ELISA was used to determine the plasma levels of cytokines (TNF-±, IL-1, IL-6) and receptors sTNF-R1 (R & D Systems). Plasma levels of cytokines of 10 healthy volunteers were also determined. RESULTS: The concentration of TNF-± before (5.8 ± 2.8 pg.mL-¹) was significantly higher than after treatment with tramadol (4.8 ± 2.1 pg.mL-1; p = 0.04, Mann-Whitney test). The levels of IL-1², IL-6, and sTNF-R1 before and after treatment with tramadol showed no significant differences. Plasma levels of TNF-± (healthy individuals: 1.4 ± 0.5; pain patients: 5.8 ± 2.8 pg.mL-1; p = 0.01) and IL-6 (healthy individuals: 1.2 ± 0.8; pain patients: 3.5 ± 2.6 pg.mL-1; p = 0.01) were significantly higher in patients with neuropathic pain, Mann-Whitney Test. CONCLUSIONS: In patients with herniated intervertebral disks and carpal tunnel syndrome, plasma levels of TNF-± and IL-6 were higher than in healthy volunteers, while differences in the concentrations of sTNF-R and IL-1² were not observed. Plasma levels of TNF-±, but not of IL-6, sTNF-R, and IL-1², decreased after treatment with tramadol (100 mg every 12 hours). JUSTIFICATIVA Y OBJETIVOS: Las interleucinas proinflamatorias tienen una función importante en la fisiopatología de los síndromes dolorosos neuropáticos. El objetivo de este estudio, fue evaluar los niveles plasmáticos de interleucinas proinflamatorias antes y después del tratamiento con tramadol en pacientes con hernia de disco y síndrome del túnel del carpo, y compararlos con individuos normales. MÉTODO: Se investigaron 38 pacientes con dolor neuropático por hernia de disco o síndrome del túnel del carpo. Todos los pacientes fueron tratados con tramadol de liberación controlada (100 mg en 12h) durante 10 días. Se realizaron muestras de sangre venosa (5 mL), por la mañana, antes del tratamiento y en el 11º día, y las mismas se almacenaron para ser analizadas (-70ºC). Se utilizaron test enzimáticos ELISA para la dosificación de las interleucinas plasmáticas (TNF-±, IL-1, IL-6) y receptores sTNF-R1, (R & D Systems). Se realizó la dosificación de interleucinas en suero de 10 voluntarios sanos. RESULTADOS: La concentración de TNF-± antes (5,8 ± 2,8 pg.mL-1) fue significativamente mayor que después del tramadol (4,8 ± 2,1 pg.mL-1; p = 0,04, Test de Mann-Whitney). No hubo diferencia significativa de IL-1², IL-6 y sTNF-R1 antes y después del tratamiento. Las concentraciones plasmáticas de TNF-± (sanos: 1,4 ± 0,5; pacientes con dolor: 5,8 ± 2,8 pg.mL-1; p = 0.01) y IL-6 (sanos: 1,2 ± 0,8; pacientes con dolor: 3.5 ± 2,6 pg.mL-1; p = 0,01) fueron significativamente mayores en los pacientes con dolor neuropático que en los voluntarios, test de Mann-Whitney. CONCLUSIONES: En los pacientes con hernia discal y síndrome del túnel del carpo, las concentraciones plasmáticas de TNF-± y IL-6, fueron más elevadas que en los voluntarios sanos, no habiendo ninguna diferencia en las concentraciones de sTNF-R y IL-1². Hubo una reducción de la concentración plasmática de TNF-±; después del tratamiento con tramadol (100 mg en 12h), pero no de IL-6 sTNF-R y IL-1². UFBA Anestesiologia UNIFESP UNIFESP Disciplina de Anestesiologia, Dor e Terapia Intensiva UFBA Laboratório de Imunologia UFBA UNIFESP, Disciplina de Anestesiologia, Dor e Terapia Intensiva SciELO
- Published
- 2009
214. Leishmaniose mucosa: aspectos clínicos e epidemiológicos
- Author
-
Lessa,Marcus Miranda, Lessa,Hélio Andrade, Castro,Thomas W. N., Oliveira,Adja, Scherifer,Albert, Machado,Paulo, and Carvalho,Edgar M.
- Subjects
leishmaniose mucosa ,leishmaniose ,doença granulomatosa - Abstract
A leishmaniose tem sido documentada em diversos países, sendo estimada uma prevalência mundial de 12 milhões, com 400.000 casos novos de doença por ano. A leishmaniose tegumentar americana encontra-se situada entre as grandes endemias existentes no Brasil e na América Latina. OBJETIVO: O objetivo deste estudo é complementar o conhecimento sobre leishmaniose mucosa, apresentando a experiência dos Serviços de Imunologia e de Otorrinolaringologia do Hospital Universitário Professor Edgar Santos da Universidade Federal da Bahia. COMENTÁRIOS: A leishmaniose cutânea é a forma mais comum de leishmaniose tegumentar americana, contudo, concomitantemente ou após anos de doença cutânea podem ocorrer lesões mucosas. A leishmaniose mucosa é causada principalmente pela L. braziliensis braziliensis e, apesar de a mucosa nasal ser a área principalmente acometida, lesões podem também ser documentadas nos lábios, boca, na faringe e na laringe. Fatores do parasito, bem como da resposta imune do hospedeiro podem estar envolvidos na patogênese da lesão tissular na leishmaniose mucosa.
- Published
- 2007
215. Colaboradores
- Author
-
Abrams, Charles S., Accurso, Frank J., Adler, Ronald S., Akin, Cem, Aksamit, Allen J., Jr., Al-Awqati, Qais, Allos, Ban Mishu, Altshuler, David, Aminoff, Michael, Anderson, Jeffrey L., Anderson, Larry J., Antony, Aśok C., Appel, Gerald B., Appelbaum, Frederick R., Apte, Suneel S., Armitage, James O., Arnaout, M. Amin, Arnold, Robert M., Atkins, David, Atkinson, John P., Bacon, Bruce R., Baddour, Larry M., Bagby, Grover C., Bain, Barbara J., Bajorin, Dean F., Baloh, Robert W., Barasch, Jonathan, Barbano, Richard L., Barrett-Connor, Elizabeth, Bartholomew, John R., Barton, Mary, Basner, Robert C., Baum, Stephen G., Bausch, Daniel G., Bayer, Arnold S., Bazari, Hasan, Beigel, John H., Beller, George A., Bennett, Robert M., Berger, Joseph R., Berk, Paul D., Berliner, Nancy, Bernat, James L., Bierman, Philip J., Bishop, Michael R., Bistrian, Bruce R., Biundo, Joseph J., Black, Adrian R., Blanke, Charles D., Blankson, Joel N., Blaser, Martin J., Blattner, William A., Bleck, Thomas P., Block, Joel A., Blom, Henk, Bodamer, Olaf A., Boden, William E., Bolognia, Jean, Bonomo, Robert A., Borish, Larry, Bosque, Patrick J., Brenner, David J., Brook, Itzhak, Brunetti, Enrico, Buchner, David M., Buffet, Pierre A., Bunn, H. Franklin, Bushinsky, David A., Bykerk, Vivian P., Calabresi, Peter A., Calfee, David P., Cameron, Douglas, Camilleri, Michael, Cannon, Grant W., Cappellini, Maria Domenica, Carabello, Blase A., Carvalho, Edgar M., Catherino, William H., Cauley, Jane A., Chalasani, Naga P., Chambers, Henry F., Cheshire, William P., Jr., Cho, Ilseung, Chockalingam, Arun, Christiani, David C., Chu, David H., Cieslak, Theodore J., Clancy, Carolyn, Clemmons, David R., Cohen, David, Cohen, Jeffrey, Cohen, Myron S., Cohen, Steven P., Cohn, Steven L., Colebunders, Robert, Connors, Joseph M., Cook, Deborah J., Cowan, Kenneth H., Craft, Joseph, Crandall, Jill Patricia, Croft, Simon L., Crothers, Kristina, Crow, Mary K., Crump, John A., Cullen, Mark R., Cunningham-Rundles, Charlotte, Damon, Inger K., Daniels, Troy E., Davidson, Nancy E., DeAngelis, Lisa M., DeCamp, Malcolm M., del Rio, Carlos, Deuster, Patricia A., Diasio, Robert B., Diemert, David J., Digre, Kathleen B., Doroshow, James H., Douglas, John M., Jr., Drazen, Jeffrey M., Dreskin, Stephen C., Drew, W. Lawrence, Drusano, George L., DuBose, Thomas D., Jr., Duffy, F. Daniel, DuPont, Herbert L., Duvic, Madeleine, Edwards, Kathryn M., Edwards, N. Lawrence, Einhorn, Lawrence H., Elin, Ronald J., Eliopoulos, George M., Elliott, Perry, Ellner, Jerrold J., Elston, Dirk M., Emanuel, Ezekiel J., Ernst, Joel D., Everson, Gregory T., Evoli, Amelia, Faigel, Douglas O., Falagas, Matthew E., Falk, Gary W., Feder, Gene, Feller-Kopman, David J., Firestein, Gary S., Fishman, Glenn I., Fleisher, Lee A., Flint, Paul W., Fogel, Evan L., Ford, Marsha D., Forsmark, Chris E., Fowler, Vance G., Jr., Franco, Manuel A., Freedman, David O., French, Martyn A., Freund, Karen, Gabay, Cem, Gage, Kenneth L., Galgiani, John N., Gallagher, Patrick G., Ganz, Leonard, Garan, Hasan, Garcia-Tsao, Guadalupe, Geisler, William M., George, Tony P., Gepstein, Lior, Gerber, Susan I., Gerding, Dale N., Gertz, Morie A., Ginder, Gordon D., Ginsberg, Jeffrey S., Ginsburg, Geoffrey S., Glogauer, Michael, Gnann, John W., Jr., Golden, Matthew R., Goldman, Lee, Goldstein, Larry B., Goodnough, Lawrence T., Gotuzzo, Eduardo H., Grady, Deborah, Grammer, Leslie C., Greco, F. Anthony, Greenberg, Harry B., Greenberg, Steven A., Griggs, Robert C., Grinberg, Lev M., Grossman, Daniel, Guay-Woodford, Lisa M., Guerrant, Richard L., Gulick, Roy M., Hagspiel, Klaus D., Hainsworth, John D., Hamsten, Anders, Hande, Kenneth R., Handsfield, H. Hunter, Hansson, Göran K., Harris, Raymond C., Hauser, Stephen Crane, Hayden, Frederick G., Heimburger, Douglas C., Hewlett, Erik L., Hift, Richard J., Hill, David R., Hill, Nicholas S., Hillis, L. David, Hirsh, Jack, Holland, Steven M., Hollenberg, Steven M., Hook, Edward W., III, Hunter, David J., Hussain, Khalid, Hyman, Steven E., Iannuzzi, Michael C., Inman, Robert D., Inouye, Sharon K., Isbister, Geoffrey K., Ison, Michael G., Jabbour, Elias, Jaff, Michael R., Jen, Joanna C., Jensen, Dennis M., Jensen, Michael D., Jensen, Robert T., Johnson, Stuart, Jordan, Richard C., Józefowicz, Ralph F., Kaler, Stephen G., Kamya, Moses R., Kao, Louise W., Kaplan, Steven A., Kastner, Daniel L., Kathiresan, Sekar, Katzka, David A., Katzman, Debra K., Kauffman, Carol A., Kaushansky, Kenneth, Kaye, Keith S., Keating, Armand, Kelley, Robin K., Kern, Morton, Keusch, Gerald T., Khuri, Fadlo R., Kim, David H., Kim, Matthew, Kirchhoff, Louis V., Knopman, David S., Knox, Tamsin A., Kontoyiannis, D.P., Koppel, Barbara S., Korenblat, Kevin M., Korf, Bruce R., Korman, Neil J., Kortepeter, Mark G., Kovacs, Joseph A., Kovacs, Thomas O., Kraft, Monica, Kramer, Christopher M., Krasnewich, Donna M., Krause, Peter J., Kuemmerle, John F., Kuipers, Ernst J., Ladenson, Paul W., Laheru, Daniel, Landry, Donald W., Lang, Anthony E., Lange, Richard A., Lederle, Frank A., Lee, Thomas H., Lee, William M., Leggett, James E., Levin, Stuart, Levine, Stephanie M., Lichtenstein, Gary R., Lim, Henry W., Lima, Aldo A.M., Ling, Geoffrey S.F., Little, William C., Lloyd-Jones, Donald M., Lorber, Bennett, Low, Donald E., Lucey, Daniel R., Lupski, James R., Lyness, Jeffrey M., Lytle, Bruce W., MacKenzie, C. Ronald, MacMillan, Harriet L., Madoff, Robert D., Maldarelli, Frank, Malhotra, Atul, Manary, Mark J., Mancini, Donna, Mandell, Lionel A., Manu, Peter, Marelli, Ariane, Mariette, Xavier, Marks, Andrew R., Marr, Kieren A., Marrie, Thomas J., Martin, Paul, Mason, Joel B., Masur, Henry, Matteson, Eric L., Matthay, Michael A., Maurer, Toby A., Mayer, Emeran A., Mayer, Stephan A., McClave, Stephen A., McCool, F. Dennis, McCulloch, Charles E., McKenna, William J., McLaughlin, Vallerie, McMurray, John J.V., McQuaid, Kenneth R., Michel, Marc, Mink, Jonathan W., Mitch, William E., Molitch, Mark E., Molitoris, Bruce A., Montoya, Jose G., Morris, Alison, Moy, Ernest, Muehlenbachs, Atis, Murr, Andrew H., Musher, Daniel M., Myerburg, Robert J., Nagamani, Sandesh C.S., Naides, Stanley J., Naka, Yoshifumi, Nash, Theodore E., Nath, Avindra, Neilson, Eric G., Neinstein, Lawrence S., Nelson, Lewis S., Nestler, Eric J., Newman, Anne B., Newman, Thomas B., Nichols, William L., Nicolle, Lindsay E., Nieman, Lynnette K., Niewoehner, Dennis E., Norrby, S. Ragnar, O’Brien, Susan, O’Connor, Christopher M., O’Connor, Francis G., O’Connor, Patrick G., O’Dell, James R., O’Donnell, Anne E., Oh, Jae K., Olgin, Jeffrey E., Orenstein, Walter A., Osmon, Douglas R., Otto, Catherine M., Papania, Mark, Pappas, Peter G., Pasricha, Pankaj Jay, Paterson, David L., Patrono, Carlo, Pawlotsky, Jean-Michel, Pearson, Richard D., Perl, Trish M., Perlman, Adam, Petri, William A., Jr., Pfeffer, Marc A., Pickhardt, Perry J., Pisetsky, David S., Posner, Marshall R., Powell, Frank, Pyeritz, Reed E., Quinn, Thomas C., Radhakrishnan, Jai, Rafailidis, Petros I., Raghu, Ganesh, Ragni, Margaret, Raja, Srinivasa N., Rajkumar, S. Vincent, Ralston, Stuart H., Raoult, Didier, Rebar, Robert W., Reboli, Annette C., Reddy, K. Rajender, Redelmeier, Donald A., Reef, Susan E., Resnick, Neil M., Reuben, David B., Rivers, Emanuel P., Rogers, Joseph G., Rolain, Jean-Marc, Romero, José R., Rosene-Montella, Karen, Rosenthal, Philip J., Rothenberg, Marc E., Russell, James A., Rustgi, Anil K., Rusyniak, Daniel E., Salata, Robert A., Salmon, Jane E., Salvana, Edsel Maurice T., Santos, Renato M., Sawka, Michael N., Scanlon, Paul D., Scanzello, Carla, Schafer, Andrew I., Schaffner, William, Scheld, W. Michael, Schiff, Manuel, Schilsky, Michael L., Schooley, Robert T., Schriger, David L., Schroeder, Steven A., Schuchter, Lynn M., Schulman, Sam, Schwartz, Lawrence B., Seas, Carlos, Seifert, Steven A., Seifter, Julian L., Selcen, Duygu, Semenkovich, Clay F., Semrad, Carol E., Shamoon, Harry, Shaw, James C., Shaw, Pamela J., Sheridan, Robert L., Sherman, Stuart, Shy, Michael E., Sidransky, Ellen, Siegel, Richard M., Siliciano, Robert F., Simberkoff, Michael S., Simel, David L., Singh, Kamaljit, Skorecki, Karl, Slotki, Itzchak, Slutsky, Arthur S., Small, Eric J., Smetana, Gerald W., Southwick, Frederick S., Spiegel, Allen M., Spiera, Robert F., Spinola, Stanley M., Spriggs, David, Stankiewicz, Paweł, Stark, Paul, Steensma, David P., Steinberg, Martin H., Steiner, Theodore S., Stephens, David S., Stevens, David A., Stoller, James K., Stone, John H., Stone, Richard M., Strikas, Raymond A., Su, Edwin P., Sutter, Roland W., Swerdloff, Ronald S., Swygard, Heidi, Sykes, Megan, Tanofsky-Kraff, Marian, Tarlo, Susan M., Taylor, Victoria M., Tefferi, Ayalew, Teirstein, Paul S., Telford, Sam R., III, Thakker, Rajesh V., Tosti, Antonella, Trehan, Indi, Turner, Ronald B., Uldrick, Thomas S., Valeri, Anthony M., Varga, John, Vaughn, Bradley V., Venook, Alan P., Verbalis, Joseph G., Victor, Ronald G., Vincent, Angela, Wachter, Robert M., Wagner, Edward H., Walsh, Edward E., Walsh, Thomas J., Walston, Jeremy D., Wang, Christina, Wanke, Christine, Wasserman, Stephen I., Weber, Thomas J., Weinberg, Geoffrey A., Weinstein, David A., Weinstein, Robert S., Weiss, Roger D., Weisse, Martin, Weitz, Jeffrey I., Wells, Samuel A., Jr., Wenzel, Richard P., Werth, Victoria P., West, Sterling G., White, A. Clinton, Jr., White, Christopher J., White, Perrin C., Whitley, Richard J., Whyte, Michael P., Wiebe, Samuel, Wiener-Kronish, Jeanine P., Wijdicks, Eelco F.M., Wilber, David J., Winikoff, Beverly, Wormser, Gary P., Yanoff, Myron, Yarchoan, Robert, Young, Neal S., Young, William F., Jr., Yu, Alan S.L., Zaki, Sherif R., Zeidel, Mark L., Ziegler, Thomas R., and Zimetbaum, Peter
- Published
- 2017
- Full Text
- View/download PDF
216. 355 - Esquistossomíase (Bilharziose)
- Author
-
Carvalho, Edgar M. and Lima, Aldo A.M.
- Published
- 2017
- Full Text
- View/download PDF
217. Associação do anticorpo anticitrulina e gravidade da artrite reumatóide
- Author
-
Silva, Aldifran Ferreira da, Matos, Afonso Napoleão, Lima, Áurea Maria Santana, Lima, Elízia Fernandes, Correa, Maria Isabel Campos de Couto, and Carvalho, Edgar M.
- Subjects
musculoskeletal diseases ,rheumatoid arthritis ,anti-CCP ,fator reumatóide ,autoantibodies ,artrite reumatóide ,auto-anticorpos ,HAQ ,skin and connective tissue diseases ,rheumatoid factor - Abstract
OBJETIVOS: Avaliar a associação do anticorpo antipeptídeo citrulinado cíclico (anti-CCP) com distintos parâmetros clínicos, sorológicos e radiológicos. MÉTODOS: Anti-CCP e fator reumatóide (FR) foram pesquisados no soro de 100 pacientes com artrite reumatóide (AR). A atividade da doença foi definida por meio de um índice combinado compreendendo cinco parâmetros: número de juntas inflamadas, número de juntas doloridas, rigidez matinal, escala visual analógica (EVA) de dor e velocidade de hemossedimentação (VHS). A capacidade funcional foi medida pelo índice HAQ (Health Assessment Questionnaire) e a classe funcional foi determinada mediante aplicação dos critérios revisados do American College of Rheumatology (ACR), de 1991. Erosão e pinçamento articular foram graduados pelo índice de Sharp modificado. A análise estatística empregou os testes do Qui-quadrado, Mann Whitney e Kruskal-Wallis. RESULTADOS: Nenhum dos dois anticorpos demonstrou associação significativa com atividade da doença, sexo, idade de início da doença, presença de nódulos subcutâneos e síndrome de Sjögren. A média de idade foi significativamente menor nos pacientes com AR anti-CCP positivos. A positividade para o FR e anti-CCP foi maior nos pacientes com AR com menos de 50 anos em comparação com os pacientes com mais de 50 anos. A AR de início recente (< 2 anos) não se associou a uma maior prevalência de soropositividade para o anti-CCP e FR. O anti-CCP apresentou uma correlação positiva moderada com o FR. Houve uma correlação direta entre o anti-CCP e a VHS e a proteína C reativa (PCR). Houve também uma correlação direta entre o FR, a VHS e a PCR. A classificação funcional dos critérios revisados do ACR de 1991 não se associou a qualquer dos dois auto-anticorpos. O índice de HAQ não se correlacionou com a atividade da doença, duração da doença, positividade do FR e atividade medida pela PCR, mas associou-se nitidamente à positividade do anti-CCP e à atividade medida pelo VHS. Os índices de Sharp para erosão e pinçamento se associaram à positividade do anti-CCP. Tal associação não foi evidenciada com a positividade do FR. CONCLUSÃO: Embora o anti-CCP apresente boas propriedades diagnósticas, ele não apresenta associação com a atividade da doença, sexo, idade de início da doença, tempo de evolução de doença, presença de nódulos subcutâneos, síndrome de Sjögren ou classificação funcional do ACR de 1991. Houve associação da positividade do anti-CCP com a menor idade dos pacientes, fator reumatóide, VHS, PCR, índice de HAQ e índices de Sharp para erosão e pinçamento. OBJECTIVE: Evaluate the association of Anti-Cyclic Citrullinated Peptide Antibody (anti-CCP) with distinct clinic, serological and radiological parameters. METHODS: anti-CCP and rheumatoid factor (RF) were determined in the serum of 100 patients with rheumatoid arthritis (RA). Disease activity was defined by means of a combined index with five parameters: number of swollen joints, number of painful joints, morning stiffness, pain visual analogue scale (VAS), and erythrocyte sedimentation rate (ESR). Functional capacity was measured by (Health Assessment Questionnaire) HAQ index and the functional class was ascribed according to American College of Rheumatology criteria (1991). Articular erosion and narrowing were estimated by the modified Sharp's index. Statistical analysis was performed by chi-square, Mann-Whitney, and Kruskal-Wallis tests. A value of less 0.05 was considered significant. RESULTS: None of the two antibodies showed association with disease flare, gender, age in the time of diagnosis, secondary Sjögren's syndrome or subcutaneous nodules. The mean age was significantly lower in RA patients with positive anti-CCP. The RF and anti-CCP positivity was higher in RA patients below 50 years old than patients above 50 years old. The early RA, up to 2 years of evolution, was not associated with a higher prevalence of anti-CCP and RF reactivity. Anti-CCP showed direct moderate correlation to RF and also direct correlation to ESR and CRP. FR reactivity showed direct correlation to ESR and CRP. Functional class showed no association with neither autoantibodies. HAQ index showed correlation with anti-CCP-positive patients and activity assessed by ESR, but did not show association with disease activity, disease duration, presence of the RF and activity assessed by ESR. Sharp's erosion and narrowing index showed association to presence of anti-CCP, but not with RF positive patients. CONCLUSIONS: Although anti-CCP displays good diagnosis properties for RA, it appears not to be associated with activity disease, gender, age at the disease beginning, disease evolution, presence of the secondary Sjögren's syndrome and subcutaneous nodules or functional class. Anti-CCP reactivity showed association with early patients, RF positivity, ESR, CRP, HAQ index or Sharp'erosion and narrowing index.
- Published
- 2006
218. Association of cytokines, neurological disability, and disease duration in HAM/TSP patients
- Author
-
Muniz,André Luiz, Rodrigues Jr.,Waldyr, Santos,Silvane B., Jesus,Amélia R. de, Porto,Aurélia F., Castro,Néviton, Oliveira-Filho,Jamary, Almeida,Juliana Passos, Moreno-Carvalho,Otávio, and Carvalho,Edgar M.
- Subjects
human T-lymphotropic virus 1 ,endocrine system ,nervous system diseases ,immune system diseases ,hemic and lymphatic diseases ,neurological disability ,virus diseases ,cytokines - Abstract
OBJECTIVE: To identify clinical and immunological markers associated with HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP). METHOD: 237 HTLV-I infected individuals were clinically assessed. They were classified according to the Expanded Disability Status Scale (EDSS) and Osames Motor Disability Score (OMDS). Cytokine levels were determined in HTLV-I seropositive individuals. RESULTS: 37 patients had HAM/TSP. There was a correlation between the degrees of disability assessed by both scales. There was also a correlation between the duration of HAM/TSP and the severity of disability assessed by either EDSS or OMDS. Higher levels of IFN-gamma were detected in unstimulated peripheral blood mononuclear cells (PBMC) from HAM/TSP patients as compared with HTLV-I carriers. CONCLUSION: This study shows the validity of the neurological scales to classify the degree of neurological disability in HTLV-I carriers and suggests a progressive behavior of HAM/TSP. This study also shows that IFN-gamma in PBMC supernatants are markers of HAM/TSP.
- Published
- 2006
219. Diagnostic value of anti-cyclic citrullinated peptide antibody in rheumatoid arthritis
- Author
-
Silva,Aldifran Ferreira da, Matos,Afonso Napoleão, Lima,Áurea Maria Santana, Lima,Elízia Fernandes, Gaspar,Antônio Pinheiro, Braga,José Antônio Fortes, and Carvalho,Edgar M.
- Subjects
rheumatoid arthritis ,autoantibodies ,artrite reumatóide ,antipeptídeos citrulinados cíclicos ,auto-anticorpos ,diagnostic ,anti-cyclic citrullinated peptide ,diagnóstico - Abstract
INTRODUÇÃO: a artrite reumatóide (AR) é considerada uma das doenças auto-imunes mais freqüentes, mas um teste viável e específico para seu diagnóstico não está disponível. OBJETIVOS: avaliar a eficiência diagnóstica de um novo teste comercial realizado pela técnica de ELISA (Immunoscan RA; EuroDiagnostica), que detecta o anticorpo antipeptídeo citrulinado cíclico (anti-CCP), para o diagnóstico da AR. MÉTODOS: anticorpo anti-CCP e fator reumatóide (FR) foram pesquisados no soro de 486 pacientes: 100 pacientes com AR e 386 controles, incluindo indivíduos normais, pacientes com outras doenças reumáticas e não-reumáticas, bem como pacientes com doenças infecciosas. Avaliação comparativa do desempenho diagnóstico dos testes foi feita mediante cálculo dos diversos índices diagnósticos e da curva ROC (receiver operator characteristic). A análise estatística foi feita utilizando os testes do qui-quadrado, exato de Fisher e Mann-Whitney. RESULTADOS: ao ponto de corte de 25 UI/ml, o anti-CCP apresentou sensibilidade de 68% (IC 95%, 57,8-76,8%), especificidade de 97,7% (IC 95%, 95,5-98,8%), valor preditivo positivo (VPP) de 88,3% (IC 95%, 78,5-94,2%), valor preditivo negativo (VPN) de 92,2% (IC 95%, 89-94,5%) e razão de verossimilhança (RV) de 29,2% (IC 95%, 15,1-56,4%). Os pacientes com AR anti-CCP positivos apresentavam titulação média igual a 920,7 UI/ml (variação, 70,5- 2000 UI/ml). Pacientes não-reumatóides anti-CCP positivos apresentavam titulação média de 38,7 UI/ml (variação, 29,5-47,4 UI/ml). O desempenho do anti-CCP, avaliado através da curva ROC, foi superior ao do FR. O FR apresentou uma sensibilidade maior (91%) e uma especificidade menor (78,8%) do que o anti-CCP. Quando os dois anticorpos foram usados em conjunto, a especificidade foi de 99,5%. CONCLUSÃO: o anti-CCP foi o teste de melhor desempenho diagnóstico para a AR e o mais específico. Pode ser útil quando realizado concomitantemente com o FR nas situações de difícil diagnóstico. INTRODUCTION: Rheumatoid arthritis (RA) is one of the most common autoimmune rheumatic diseases, but a specific and reproducible test for its diagnosis is still lacking. OBJECTIVES: To evaluate the diagnostic efficiency of a new commercial ELISA kit in detecting anti-cyclic citrullinated peptide antibodies (anti-CCP) for the diagnosis of RA. METHODS: Anti-CCP antibodies and rheumatoid factor (RF) were determined in the serum of 486 patients: 100 patients with RA and 386 controls, including healthy subjects, patients with other non-rheumatic and rheumatic disease, as well as patients with infections disease. Comparative evaluation of diagnostic performance of anti-CCP and RF was done by calculation the several diagnostic indexes and construction of the ROC (receiver operator characteristic) curve. Statistical analysis included chi-square, Fisher exact, and Mann-Whitney's test. RESULTS: At cutoff of 25 UI/ml, anti-CCP showed sensitivity of 68% (95% CI, 57,8-76,8%), specificity of 97,7% (95% CI, 95,5-98,8%), positive predictive value (VPP) of 88,3% (95% CI, 78,5-94,2%), negative predictive value (VPN) of 92,2% (95% CI, 89-94,5%) and likelihood ratios (LR) of 29,2% (95% CI, 15,1-56,4%). Anti-CCP-positive RA patients had a mean antibody concentration of 920,7 UI/ml (range, 70,5-2000 UI/ml). Anti-CCP-positive non-RA patients had a mean antibody concentration of 38,7 UI/ml (range, 29,5-47, 4 UI/ml). The diagnostic performance of anti-CCP, as estimated by the ROC curve, was superior to that of RF. RF had a higher sensitivity (91%) and a lower specificity (78,8%) than anti-CCP. When the two antibodies were used together, specificity was 99,5%. CONCLUSION: The anti-CCP testing presented the best diagnostic performance for RA and was the most specific test. It may be useful if performance concomitantly with RF in the diagnostic difficulty.
- Published
- 2006
220. Association of cytokines, neurological disability, and disease duration in HAM/TSP patients
- Author
-
Muniz, André Luiz, Rodrigues Jr., Waldyr, Santos, Silvane B., Jesus, Amélia R. de, Porto, Aurélia F., Castro, Néviton, Oliveira-Filho, Jamary, Almeida, Juliana Passos, Moreno-Carvalho, Otávio, and Carvalho, Edgar M.
- Subjects
human T-lymphotropic virus 1 ,nervous system diseases ,doenças do sistema nervoso ,immune system diseases ,hemic and lymphatic diseases ,neurological disability ,vírus 1 linfotrópico T humano ,citocinas ,virus diseases ,incapacidade neurológica ,cytokines - Abstract
OBJECTIVE: To identify clinical and immunological markers associated with HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP). METHOD: 237 HTLV-I infected individuals were clinically assessed. They were classified according to the Expanded Disability Status Scale (EDSS) and Osame’s Motor Disability Score (OMDS). Cytokine levels were determined in HTLV-I seropositive individuals. RESULTS: 37 patients had HAM/TSP. There was a correlation between the degrees of disability assessed by both scales. There was also a correlation between the duration of HAM/TSP and the severity of disability assessed by either EDSS or OMDS. Higher levels of IFN-gamma were detected in unstimulated peripheral blood mononuclear cells (PBMC) from HAM/TSP patients as compared with HTLV-I carriers. CONCLUSION: This study shows the validity of the neurological scales to classify the degree of neurological disability in HTLV-I carriers and suggests a progressive behavior of HAM/TSP. This study also shows that IFN-gamma in PBMC supernatants are markers of HAM/TSP. OBJETIVO: Identificar marcadores clínicos e imunológicos associados com a mielopatia associada ao HTLV-I/paraparesia espástica tropical (MAH/PET). MÉTODO: 237 indivíduos infectados pelo HTLV-I foram clinicamente avaliados. Eles foram classificados de acordo com a escala expandida do estado de incapacidade de Kurtzke (EDSS) e escala de incapacidade motora de Osame (OMDS). Níveis de citocinas foram determinados nos indivíduos. RESULTADOS: 37 pacientes tinham MAH/PET. Houve correlação entre os graus de incapacidade pelas escalas. Houve também correlação entre a duração da MAH/PET e o grau da incapacidade pelas escalas. Níveis elevados de IFN-gama foram detectados em células mononucleares de sangue periférico (CMSP) não estimuladas de pacientes com MAH/PET quando comparados com indivíduos HTLV-I positivos assintomáticos. CONCLUSÃO: Os dados demonstram a validade das escalas neurológicas para classificar o grau de incapacidade neurológica em portadores do HTLV-I e sugerem o comportamento progressivo da MAH/PET. Este estudo também demonstra que os níveis de IFN-gama em sobrenadante de CMSP são marcadores da MAH/PET.
- Published
- 2006
221. Associação do anticorpo anticitrulina e gravidade da artrite reumatóide
- Author
-
Silva,Aldifran Ferreira da, Matos,Afonso Napoleão, Lima,Áurea Maria Santana, Lima,Elízia Fernandes, Correa,Maria Isabel Campos de Couto, and Carvalho,Edgar M.
- Subjects
anti-CCP ,fator reumatóide ,artrite reumatóide ,auto-anticorpos ,HAQ - Abstract
OBJETIVOS: Avaliar a associação do anticorpo antipeptídeo citrulinado cíclico (anti-CCP) com distintos parâmetros clínicos, sorológicos e radiológicos. MÉTODOS: Anti-CCP e fator reumatóide (FR) foram pesquisados no soro de 100 pacientes com artrite reumatóide (AR). A atividade da doença foi definida por meio de um índice combinado compreendendo cinco parâmetros: número de juntas inflamadas, número de juntas doloridas, rigidez matinal, escala visual analógica (EVA) de dor e velocidade de hemossedimentação (VHS). A capacidade funcional foi medida pelo índice HAQ (Health Assessment Questionnaire) e a classe funcional foi determinada mediante aplicação dos critérios revisados do American College of Rheumatology (ACR), de 1991. Erosão e pinçamento articular foram graduados pelo índice de Sharp modificado. A análise estatística empregou os testes do Qui-quadrado, Mann Whitney e Kruskal-Wallis. RESULTADOS: Nenhum dos dois anticorpos demonstrou associação significativa com atividade da doença, sexo, idade de início da doença, presença de nódulos subcutâneos e síndrome de Sjögren. A média de idade foi significativamente menor nos pacientes com AR anti-CCP positivos. A positividade para o FR e anti-CCP foi maior nos pacientes com AR com menos de 50 anos em comparação com os pacientes com mais de 50 anos. A AR de início recente (< 2 anos) não se associou a uma maior prevalência de soropositividade para o anti-CCP e FR. O anti-CCP apresentou uma correlação positiva moderada com o FR. Houve uma correlação direta entre o anti-CCP e a VHS e a proteína C reativa (PCR). Houve também uma correlação direta entre o FR, a VHS e a PCR. A classificação funcional dos critérios revisados do ACR de 1991 não se associou a qualquer dos dois auto-anticorpos. O índice de HAQ não se correlacionou com a atividade da doença, duração da doença, positividade do FR e atividade medida pela PCR, mas associou-se nitidamente à positividade do anti-CCP e à atividade medida pelo VHS. Os índices de Sharp para erosão e pinçamento se associaram à positividade do anti-CCP. Tal associação não foi evidenciada com a positividade do FR. CONCLUSÃO: Embora o anti-CCP apresente boas propriedades diagnósticas, ele não apresenta associação com a atividade da doença, sexo, idade de início da doença, tempo de evolução de doença, presença de nódulos subcutâneos, síndrome de Sjögren ou classificação funcional do ACR de 1991. Houve associação da positividade do anti-CCP com a menor idade dos pacientes, fator reumatóide, VHS, PCR, índice de HAQ e índices de Sharp para erosão e pinçamento.
- Published
- 2006
222. Cutaneous Manifestations of Human and Murine Leishmaniasis.
- Author
-
Scorza, Breanna M., Carvalho, Edgar M., and Wilson, Mary E.
- Subjects
- *
CUTANEOUS manifestations of general diseases , *LEISHMANIASIS treatment , *PATHOGENIC protozoa , *SKIN diseases , *PATHOLOGY , *THERAPEUTICS - Abstract
The leishmaniases are diseases caused by pathogenic protozoan parasites of the genus Leishmania. Infections are initiated when a sand fly vector inoculates Leishmania parasites into the skin of a mammalian host. Leishmania causes a spectrum of inflammatory cutaneous disease manifestations. The type of cutaneous pathology is determined in part by the infecting Leishmania species, but also by a combination of inflammatory and anti-inflammatory host immune response factors resulting in different clinical outcomes. This review discusses the distinct cutaneous syndromes described in humans, and current knowledge of the inflammatory responses associated with divergent cutaneous pathologic responses to different Leishmania species. The contribution of key hematopoietic cells in experimental cutaneous leishmaniasis in mouse models are also reviewed and compared with those observed during human infection. We hypothesize that local skin events influence the ensuing adaptive immune response to Leishmania spp. infections, and that the balance between inflammatory and regulatory factors induced by infection are critical for determining cutaneous pathology and outcome of infection. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
223. CD8+ T cell cytotoxicity mediates pathology in the skin by inflammasome activation and IL-1β production.
- Author
-
Novais, Fernanda O., Carvalho, Augusto M., Clark, Megan L., Carvalho, Lucas P., Beiting, Daniel P., Brodsky, Igor E., Carvalho, Edgar M., and Scott, Phillip
- Subjects
CELL-mediated cytotoxicity ,T cells ,IMMUNOPATHOLOGY ,LEISHMANIA ,INFLAMMATION - Abstract
Deregulated CD8+ T cell cytotoxicity plays a central role in enhancing disease severity in several conditions. However, we have little understanding of the mechanisms by which immunopathology develops as a consequence of cytotoxicity. Using murine models of inflammation induced by the protozoan parasite leishmania, and data obtained from patients with cutaneous leishmaniasis, we uncovered a previously unrecognized role for NLRP3 inflammasome activation and IL-1β release as a detrimental consequence of CD8+ T cell-mediated cytotoxicity, ultimately resulting in chronic inflammation. Critically, pharmacological blockade of NLRP3 or IL-1β significantly ameliorated the CD8+ T cell-driven immunopathology in leishmania-infected mice. Confirming the relevance of these findings to human leishmaniasis, blockade of the NLRP3 inflammasome in skin biopsies from leishmania-infected patients prevented IL-1β release. Thus, these studies link CD8+ T cell cytotoxicity with inflammasome activation and reveal novel avenues of treatment for cutaneous leishmaniasis, as well as other of diseases where CD8+ T cell-mediated cytotoxicity induces pathology. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
224. Mecanismos de resposta imune às infecções
- Author
-
Machado, Paulo R. L., Araújo, Maria Ilma A. S., Carvalho, Lucas, and Carvalho, Edgar M.
- Subjects
helmintíase ,helminthiasis ,imunidade ,infecções por protozoários ,immunity ,infection ,viroses ,bacterial infections ,infecções bacterianas ,infecção ,virus diseases ,protozoan infections ,mycoses ,imunidade natural ,micoses ,infecção/imunologia - Abstract
O conhecimento dos principais mecanismos de defesa imune contra os diversos agentes infecciosos permite a compreensão da patogênese das doenças infectoparasitárias e das várias estratégias do hospedeiro e do parasita. O sistema imunológico atua numa rede de cooperação, envolvendo a participação de muitos componentes estruturais, moleculares e celulares. Nesse cenário encontra-se o delicado equilíbrio entre a saúde e a doença, em que tanto a deficiência quanto o exagero resultam em dano tecidual. Este artigo explora esses aspectos e algumas abordagens terapêuticas que surgem desse entendimento. Knowledge acquired about the main immune mechanisms protecting against various infectious agents leads to a better understanding of the pathogenesis of infectious/parasitic diseases and of various strategies specific to the host and parasite. The immune system performs along a cooperation network, which involves the participation of several structural, molecular and cellular components. The fine balance between health and disease is found in this scenario, in which deficiency as much as excess may result in tissue damage. This article explores such aspects and a number of therapies arising from the knowledge acquired.
- Published
- 2004
225. Association of Type 2 Cytokines with Hepatic Fibrosis in Human Schistosoma mansoni Infection
- Author
-
Silva, Ângela Maria da, Jesus, Amélia Maria Ribeiro de, Magalhães, Andréa, Miranda, Delfin Gonzalez, Miranda, Roberval Gonzalez, Araújo, Maria Ilma, Jesus, Adriana Almeida de, Santana, Luciana Barros de, Pearce, Edward, and Carvalho, Edgar M.
- Subjects
Fibrose hepática ,Esquistossomose ,Schistosoma mansoni - Abstract
The objective of this study was to evaluate the role of cytokines in hepatic fibrosis in the prehepatosplenic and early hepatosplenic stages of schistosomiasis mansoni. Hepatic fibrosis was classified by ultrasonography of 94 patients. Immunological evaluation was performed by the measurement of secreted cytokines (interleukin-5 [IL-5], IL-10, IL-13, gamma interferon, tumor necrosis factor alpha, and transforming growth factor β) in peripheral blood mononuclear cells (PBMC) stimulated by Schistosoma mansoni antigens. Significantly, higher levels of IL-5, IL-10, and IL-13 were found in supernatants of soluble egg antigen-stimulated PBMC from subjects with degree III hepatic fibrosis compared to patients with degree I or II fibrosis. Significant increases in IL-5 and IL-13 levels were also observed in some of the subjects who remained untreated for 1 year following initial assessment and developed more serious fibrosis during this period. The data suggest a role for type 2 cytokines in hepatic fibrosis in human schistosomiasis mansoni.
- Published
- 2004
226. Tr-1–Like CD4+CD25−CD127−/lowFOXP3− Cells Are the Main Source of Interleukin 10 in Patients With Cutaneous Leishmaniasis Due to Leishmania braziliensis
- Author
-
Costa, Diego L., primary, Cardoso, Tiago M., additional, Queiroz, Adriano, additional, Milanezi, Cristiane M., additional, Bacellar, Olívia, additional, Carvalho, Edgar M., additional, and Silva, João S., additional
- Published
- 2014
- Full Text
- View/download PDF
227. Intermediate Monocytes Contribute to Pathologic Immune Response inLeishmania braziliensisInfections
- Author
-
Passos, Sara, primary, Carvalho, Lucas P., additional, Costa, Rúbia S., additional, Campos, Taís M., additional, Novais, Fernanda O., additional, Magalhães, Andréa, additional, Machado, Paulo R. L., additional, Beiting, Daniel, additional, Mosser, David, additional, Carvalho, Edgar M., additional, and Scott, Phillip, additional
- Published
- 2014
- Full Text
- View/download PDF
228. Clinical and Immunological Outcome in Cutaneous Leishmaniasis Patients Treated with Pentoxifylline
- Author
-
Brito, Graça, primary, Machado, Paulo R. L., additional, Dourado, Mayra, additional, Queiroz, Adriano, additional, Carvalho, Lucas P., additional, Celestino, Daniela, additional, Passos, Sara, additional, Polari, Ludmila, additional, and Carvalho, Edgar M., additional
- Published
- 2014
- Full Text
- View/download PDF
229. Dendritic Cell Profile Induced bySchistosoma mansoniAntigen in Cutaneous Leishmaniasis Patients
- Author
-
Lopes, Diego Mota, primary, Fernandes, Jamille Souza, additional, Cardoso, Thiago Marconi de Souza, additional, Bafica, Aline Michele Barbosa, additional, Oliveira, Sérgio Costa, additional, Carvalho, Edgar M., additional, Araujo, Maria Ilma, additional, and Cardoso, Luciana Santos, additional
- Published
- 2014
- Full Text
- View/download PDF
230. Immune response, proviral load and serologic markers of neurologic disease expression in HTLV-1 infection
- Author
-
Santos, Silvane, primary, Toledo, Cristina, additional, Souza, Anselmo, additional, Tanajura, Davi, additional, Glesby, Marshall, additional, and Carvalho, Edgar M, additional
- Published
- 2014
- Full Text
- View/download PDF
231. Modulation of the immune response in HTLV-1-infections by SM29 antigen in vitro is dependent of IL-10, TGF-β and CTLA-4
- Author
-
Lima, Luciane Mota, primary, Santos, Silvane Braga, additional, Oliveira, Ricardo Riccio, additional, Cardoso, Luciana Santos, additional, Oliveira, Sergio Costa, additional, Góes, Alfredo Miranda, additional, Loukas, Alex, additional, De Carvalho, Edgar M, additional, and Araújo, Maria Ilma, additional
- Published
- 2014
- Full Text
- View/download PDF
232. Comment: seroprevalence of HTLV-1/2 among blood donors in the state of Maranhão Brazil✰✰See paper by Viana GM et al. on pages 50-3
- Author
-
Carvalho, Edgar M., primary
- Published
- 2014
- Full Text
- View/download PDF
233. Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis
- Author
-
Fernandes, Jamille Souza, primary, Araujo, Maria Ilma, additional, Lopes, Diego Mota, additional, Souza, Robson da Paixão de, additional, Carvalho, Edgar M., additional, and Cardoso, Luciana Santos, additional
- Published
- 2014
- Full Text
- View/download PDF
234. Impairment of humoral and cellular immune response to tetanus toxoid in HTLV-1 infected individuals
- Author
-
Souza, Anselmo S, primary, Amorim, Camila F, additional, Carvalho, Natália, additional, Santos, Silvane B, additional, and Carvalho, Edgar M, additional
- Published
- 2014
- Full Text
- View/download PDF
235. Prevalence and influence of tuberculosis in the neurologic manifestations of the Human T cell lymphotropic virus type 1 (HTLV-1)
- Author
-
de Lourdes Bastos, Maria, primary, Souza, Anselmo, additional, Carvalho, Natália, additional, Neves, Yuri, additional, Santos, Silvane, additional, and Carvalho, Edgar M, additional
- Published
- 2014
- Full Text
- View/download PDF
236. IL-1β Production by Intermediate Monocytes Is Associated with Immunopathology in Cutaneous Leishmaniasis
- Author
-
Santos, Daniela, Campos, Taís M., Saldanha, Maíra, Oliveira, Sergio C., Nascimento, Mauricio, Zamboni, Dario S., Machado, Paulo R., Arruda, Sérgio, Scott, Phillip, Carvalho, Edgar M., and Carvalho, Lucas P.
- Abstract
Cutaneous leishmaniasis due to Leishmania braziliensisinfection is an inflammatory disease in which skin ulcer development is associated with mononuclear cell infiltrate and high levels of inflammatory cytokine production. Recently, NLRP3 inflammasome activation and IL-1β production have been associated with increased pathology in murine cutaneous leishmaniasis. We hypothesized that cutaneous leishmaniasis patients have increased expression of NLRP3, leading to high levels of IL-1β production. In this article we show high production of IL-1β in biopsy samples and Leishmaniaantigen-stimulated peripheral blood mononuclear cells from patients infected with L. braziliensisand reduced IL-1β levels after cure. IL-1β production positively correlated with the area of necrosis in lesions and duration of the lesions. The main source of IL-1β was intermediate monocytes (CD14++CD16+). Furthermore, our murine experiments show that IL-1β production in response to L. braziliensiswas dependent on NLRP3, caspase-1, and caspase-recruiting domain (ASC). Additionally, we observed an increased expression of the NLRP3gene in macrophages and the NLRP3 protein in intermediate monocytes from cutaneous leishmaniasis patients. These results identify an important role for human intermediate monocytes in the production of IL-1β, which contributes to the immunopathology observed in cutaneous leishmaniasis patients.
- Published
- 2018
- Full Text
- View/download PDF
237. Association between urinary symptoms and quality of life in HTLV-1 infected subjects without myelopathy
- Author
-
Andrade, Rosana, primary, Tanajura, Davi, additional, Santana, Deise, additional, Santos, Dislene dos, additional, and Carvalho, Edgar M., additional
- Published
- 2013
- Full Text
- View/download PDF
238. Characterization of regulatory T cell (Treg) function in patients infected with Leishmania braziliensis
- Author
-
Costa, Diego L., primary, Guimarães, Luiz H., additional, Cardoso, Thiago M., additional, Queiroz, Adriano, additional, Lago, Ednaldo, additional, Roselino, Ana M., additional, Bacellar, Olívia, additional, Carvalho, Edgar M., additional, and Silva, João S., additional
- Published
- 2013
- Full Text
- View/download PDF
239. Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
- Author
-
Carvalho, Augusto M, primary, Magalhães, Andréa, additional, Carvalho, Lucas P, additional, Bacellar, Olívia, additional, Scott, Phillip, additional, and Carvalho, Edgar M, additional
- Published
- 2013
- Full Text
- View/download PDF
240. Spatial analysis and risk mapping of soil-transmitted helminth infections in Brazil, using Bayesian geostatistical models
- Author
-
Scholte, Ronaldo G. C., primary, Schur, Nadine, additional, Bavia, Maria E., additional, Carvalho, Edgar M., additional, Chammartin, Frédérique, additional, Utzinger, Jürg, additional, and Vounatsou, Penelope, additional
- Published
- 2013
- Full Text
- View/download PDF
241. Adult T-cell leukemia/lymphoma triggered by adalimumab
- Author
-
Bittencourt, Achilea L, primary, Oliveira, Pedro D, additional, Bittencourt, Valeria G, additional, Carvalho, Edgar M, additional, and Farre, Lourdes, additional
- Published
- 2013
- Full Text
- View/download PDF
242. Dermatological manifestations of individuals infected with human T cell lymphotropic virus type I (HTLV-I)
- Author
-
Dantas, Lorena, primary, Netto, Eduardo, additional, Glesby, Marshall J., additional, Carvalho, Edgar M., additional, and Machado, Paulo, additional
- Published
- 2013
- Full Text
- View/download PDF
243. Soluble IL‐2 receptor and beta‐2 microglobulin as possible serologic markers of neurologic disease in HTLV‐1 infection
- Author
-
Toledo‐Cornell, Cristina, primary, Santos, Silvane, additional, Orge, Gloria, additional, Glesby, Marshall J., additional, and Carvalho, Edgar M., additional
- Published
- 2013
- Full Text
- View/download PDF
244. Immunologic Response and Proviral Load in Human T-lymphotropic Virus Type 1 Infected Individuals With Erectile Dysfunction
- Author
-
Tannus, Matheus, primary, Costa, Davi T., additional, Castro, Néviton M., additional, Oliveira, Paulo, additional, Carvalho, Natália, additional, Andrade, Rosana, additional, Santos, Silvane, additional, and Carvalho, Edgar M., additional
- Published
- 2013
- Full Text
- View/download PDF
245. IL-17 Mediates Immunopathology in the Absence of IL-10 Following Leishmania major Infection
- Author
-
Gonzalez-Lombana, Claudia, primary, Gimblet, Ciara, additional, Bacellar, Olivia, additional, Oliveira, Walker W., additional, Passos, Sara, additional, Carvalho, Lucas P., additional, Goldschmidt, Michael, additional, Carvalho, Edgar M., additional, and Scott, Phillip, additional
- Published
- 2013
- Full Text
- View/download PDF
246. Novel IL33 Gene Polymorphisms Associated with Asthma Are Associated with Resistance to Schistosoma Mansoni
- Author
-
Masuko, Hironori, primary, Gao, Li, additional, Rafaels, Nicholas M., additional, Vergara, Candelaria I., additional, Huang, Lili, additional, Campbell, Monica, additional, Ruczinski, Ingo, additional, Beaty, Terri H., additional, Oliveira, Ricardo, additional, Cruz, Alvaro A., additional, Carvalho, Edgar M., additional, Mathias, Rasika A., additional, Araujo, Maria Ilma, additional, and Barnes, Kathleen C., additional
- Published
- 2013
- Full Text
- View/download PDF
247. Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis
- Author
-
Cardoso, Luciana Santos, primary, Barreto, Andréia de Souza Rocha, additional, Fernandes, Jamille Souza, additional, Oliveira, Ricardo Riccio, additional, Souza, Robson da Paixão de, additional, Carvalho, Edgar M., additional, and Araujo, Maria Ilma, additional
- Published
- 2013
- Full Text
- View/download PDF
248. Schistosoma Antigens Downmodulate the in vitro Inflammatory Response in Individuals Infected with Human T Cell Lymphotropic Virus Type 1
- Author
-
Lima, Luciane Mota, primary, Santos, Silvane Braga, additional, Oliveira, Ricardo Riccio, additional, Cardoso, Luciana Santos, additional, Oliveira, Sérgio Costa, additional, Góes, Alfredo Miranda, additional, Loukas, Alex, additional, Carvalho, Edgar M., additional, and Araújo, Maria Ilma, additional
- Published
- 2013
- Full Text
- View/download PDF
249. Immunologic Markers of Protection in Leishmania (Viannia) braziliensis Infection: A 5-Year Cohort Study.
- Author
-
Muniz, Aline C., Bacellar, Olívia, Lima Lago, Ednaldo, Carvalho, Augusto M., Carneiro, Pedro Paulo, Guimarães, Luiz Henrique, Rocha, Paulo N., Carvalho, Lucas P., Glesby, Marshall, Carvalho, Edgar M., and Lago, Ednaldo Lima
- Subjects
LEISHMANIA ,INFECTION ,CUTANEOUS leishmaniasis ,SKIN tests ,MONOCYTES ,LYMPHOCYTES - Abstract
Background: The control of Leishmania braziliensis by individuals with subclinical infection (SC) are unknown.Methods: A cohort of 308 household contacts (HCs) of patients with cutaneous leishmaniasis (CL) was established in 2010 in an endemic area and followed up for 5 years. Whole-blood cultures stimulated with soluble Leishmania antigen and a Leishmania skin test (LST) were performed in years 0, 2, and 4. The identification of the lymphocyte subsets secreting interferon (IFN) γ and the ability of monocytes to control Leishmania were determined.Results: During follow-up, 118 subjects (38.3%) had evidence of L. braziliensis infection. Of the HCs, CL was documented in 45 (14.6%), 101 (32.8%) had SC infection, and 162 (52.6%) did not have evidence of exposure to L. braziliensis The ratio of infection to disease was 3.2:1. IFN-γ production, mainly by natural killer cells, was associated with protection, and a positive LST result did not prevent development of disease. Moreover, monocytes from subjects with SC infection were less permissive to parasite penetration and had a greater ability to control L. braziliensis than cells from patients with CL.Conclusions: Protection against CL was associated with IFN-γ production, negative LST results, impaired ability of Leishmania to penetrate monocytes, and increased ability to control Leishmania growth. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
250. Chronic Chagas' Disease: Targeting the Interleukin-2 Axis and Regulatory T Cells in a Condition for Which There Is No Treatment.
- Author
-
Mengel, Jose, Cardillo, Fabíola, Pontes-de-Carvalho, Lain, Walochnik, Julia, and Carvalho, Edgar M.
- Subjects
CHAGAS' disease ,T cells - Abstract
The article discusses study on Chronic Chagas' Disease, targeting the Interleukin-2 Axis and regulatory T Cells in a condition for which there is no treatment and clinical trial that reveals treatment with benznidazole has failed to modify the clinical outcome of chronic Chagas' disease.
- Published
- 2016
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.