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806 results on '"Chai, Renjie"'

208. Pre-treatment With Fasudil Prevents Neomycin-Induced Hair Cell Damage by Reducing the Accumulation of Reactive Oxygen Species

Author

Zhang, Yanqiu, primary, Li, Wei, additional, He, Zuhong, additional, Wang, Yunfeng, additional, Shao, Buwei, additional, Cheng, Cheng, additional, Zhang, Shasha, additional, Tang, Mingliang, additional, Qian, Xiaoyun, additional, Kong, Weijia, additional, Wang, Hui, additional, Chai, Renjie, additional, and Gao, Xia, additional

Published
2019
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210. Suction‐Cup‐Inspired Adhesive Micromotors for Drug Delivery.

Author

Cai, Lijun, Zhao, Cheng, Chen, Hanxu, Fan, Lu, Zhao, Yuanjin, Qian, Xiaoyun, and Chai, Renjie

Subjects
MICROMOTORS, GASTRIC juice, COLLOIDAL crystals, SOLVENT extraction, STOMACH ulcers, ADHESIVES, HYDROGELS
Abstract

Micromotors have opened novel avenues for drug delivery due to their capacity for self‐propelling. Attempts in this field trend towards ameliorating their functions to promote their clinical applications. In this paper, an ingenious suction‐cup‐inspired micromotor is presented with adhesive properties for drug delivery in the stomach. The micromotors are fabricated by using hydrogel replicating the structure of suction‐cup‐like microparticles, which derive from self‐assembly of colloidal crystals under rapid solvent extraction, followed by loading magnesium (Mg) in the bottom spherical surface. The Mg‐loaded micromotors can realize spontaneous movement due to the continual generation of hydrogen bubbles in gastric juice. The combination of unique suction‐cup‐like structure with excellent motion performance makes the micromotor an ideal carrier for drug delivery as they can efficiently adhere to the tissue. Moreover, benefiting from the porous structure, the hydrogel micromotors exhibit a high volume‐surface ratio, which enables efficient drug loading. It is demonstrated that the suction‐cup‐inspired micromotors can adhere efficiently to the ulcer‐region in the stomach and release drugs due to their distinctive architecture and spontaneous motion, exhibiting desirable curative effect of gastric ulcer. Thus, the suction‐cup‐inspired micromotors with adhesive properties are expected to advance the development of micromotor in clinical applications. [ABSTRACT FROM AUTHOR]

Published
2022
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211. Regulation of Neural Stem Cell Proliferation and Differentiation by Graphene-Based Biomaterials

Author

Xia, Lin, Zhu, Wenjuan, Wang, Yunfeng, He, Shuangba, and Chai, Renjie

Subjects
nervous system, Article Subject
Abstract

The transplantation of neural stem cells (NSCs) has become an emerging treatment for neural degeneration. A key factor in such treatments is to manipulate NSC behaviors such as proliferation and differentiation, resulting in the eventual regulation of NSC fate. Novel bionanomaterials have shown usefulness in guiding the proliferation and differentiation of NSCs due to the materials’ unique morphological and topological properties. Among the nanomaterials, graphene has drawn increasing attention for neural regeneration applications based on the material’s excellent physicochemical properties, surface modifications, and biocompatibility. In this review, we summarize recent works on the use of graphene-based biomaterials for regulating NSC behaviors and the potential use of these materials in clinical treatment. We also discuss the limitations of graphene-based nanomaterials for use in clinical practice. Finally, we provide some future prospects for graphene-based biomaterial applications in neural regeneration.

Published
2019
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213. Cochlear implant-based electric-acoustic stimulation modulates neural stem cell-derived neural regeneration.

Author

Guo, Rongrong, Liao, Menghui, Ma, Xiaofeng, Hu, Yangnan, Qian, Xiaoyun, Xiao, Miao, Gao, Xia, Chai, Renjie, and Tang, Mingliang

Abstract

Cochlear implantation is considered to be the best therapeutic method for profound sensorineural hearing loss, but insufficient numbers of functional spiral ganglion neurons hinder the clinical effects of cochlear implantation. Stem cell transplantation has the potential to provide novel strategies for spiral ganglion neuron regeneration after injury. However, some obstacles still need to be overcome, such as low survival and uncontrolled differentiation. Several novel technologies show promise for modulating neural stem cell behaviors to address these issues. Here, a device capable of electrical stimulation was designed by combining a cochlear implant with a graphene substrate. Neural stem cells (NSCs) were cultured on the graphene substrate and subjected to electrical stimulation transduced from sound waves detected by the cochlear implant. Cell behaviors were studied, and this device showed good biocompatibility for NSCs. More importantly, electric-acoustic stimulation with higher frequencies and amplitudes induced NSC death and apoptosis, and electric-acoustic stimulation could promote NSCs to proliferate and differentiate into neurons only when low-frequency stimulation was supplied. The present study provides experimental evidence for understanding the regulatory role of electric-acoustic stimulation on NSCs and highlights the potentials of the above-mentioned device in stem cell therapy for hearing loss treatment. [ABSTRACT FROM AUTHOR]

Published
2021
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214. Designs of Biomaterials and Microenvironments for Neuroengineering

Author

Yang, Yanru, Zhang, Yuhua, Chai, Renjie, and Gu, Zhongze

Subjects
Neurons, 0301 basic medicine, Scaffold, Materials science, Tissue Engineering, Tissue Scaffolds, Biomaterial, Biocompatible Materials, Nanotechnology, Review Article, 02 engineering and technology, Neural engineering, 021001 nanoscience & nanotechnology, Biocompatible material, lcsh:RC321-571, 03 medical and health sciences, 030104 developmental biology, Neurology, Tissue engineering, Tissue scaffolds, Mechanical strength, Humans, Neurology (clinical), 0210 nano-technology, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Abstract

Recent clinical research on neuroengineering is primarily focused on biocompatible materials, which can be used to provide electroactive and topological cues, regulate the microenvironment, and perform other functions. Novel biomaterials for neuroengineering have been received much attention in the field of research, including graphene, photonic crystals, and organ-on-a-chip. Graphene, which has the advantage of high mechanical strength and chemical stability with the unique electrochemical performance for electrical signal detection and transmission, has significant potential as a conductive scaffolding in the field of medicine. Photonic crystal materials, known as a novel concept in nerve substrates, have provided a new avenue for neuroengineering research because of their unique ordered structure and spectral attributes. The “organ-on-a-chip” systems have shown significant prospects for the developments of the solutions to nerve regeneration by mimicking the microenvironment of nerve tissue. This paper presents a review of current progress in the designs of biomaterials and microenvironments and provides case studies in developing nerve system stents upon these biomaterials. In addition, we compose a conductive patterned compounded biomaterial, which could mimic neuronal microenvironment for neuroengineering by concentrating the advantage of such biomaterials.

Published
2018

215. Transcriptomic profiling of neural stem cell differentiation on graphene substrates

Author

Tang, Mingliang, primary, Li, Jian, additional, He, Li, additional, Guo, Rongrong, additional, Yan, Xiaoqian, additional, Li, Dan, additional, Zhang, Yuhua, additional, Liao, Menghui, additional, Shao, Buwei, additional, Hu, Yangnan, additional, Liu, Yun, additional, Tang, Qilin, additional, Xia, Lin, additional, Guo, Xing, additional, and Chai, Renjie, additional

Published
2019
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217. Knockdown of Foxg1 in supporting cells increases the trans-differentiation of supporting cells into hair cells in the neonatal mouse cochlea

Author

Zhang, Shasha, primary, Zhang, Yuan, additional, Dong, Ying, additional, Guo, Lingna, additional, Zhang, Zhong, additional, Shao, Buwei, additional, Qi, Jieyu, additional, Zhou, Han, additional, Zhu, Weijie, additional, Yan, Xiaoqian, additional, Hong, Guodong, additional, Zhang, Liyan, additional, Zhang, Xiaoli, additional, Tang, Mingliang, additional, Zhao, Chunjie, additional, Gao, Xia, additional, and Chai, Renjie, additional

Published
2019
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220. Frizzled-9+ Supporting Cells Are Progenitors for the Generation of Hair Cells in the Postnatal Mouse Cochlea

Author

Zhang, Shasha, primary, Liu, Dingding, additional, Dong, Ying, additional, Zhang, Zhong, additional, Zhang, Yuan, additional, Zhou, Han, additional, Guo, Lingna, additional, Qi, Jieyu, additional, Qiang, Ruiying, additional, Tang, Mingliang, additional, Gao, Xia, additional, Zhao, Chunjie, additional, Chen, Xiaoyun, additional, Qian, Xiaoyun, additional, and Chai, Renjie, additional

Published
2019
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221. THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis

Author

Zhang, Luping, primary, Gao, Yu, additional, Zhang, Ru, additional, Sun, Feifei, additional, Cheng, Cheng, additional, Qian, Fuping, additional, Duan, Xuchu, additional, Wei, Guanyun, additional, Pang, Xiuhong, additional, Chen, Penghui, additional, Chai, Renjie, additional, Yang, Tao, additional, Wu, Hao, additional, and Liu, Dong, additional

Published
2019
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223. TeraVR Empowers Precise Reconstruction of Complete 3-D Neuronal Morphology in the Whole Brain

Author

Wang, Yimin, primary, Li, Qi, additional, Liu, Lijuan, additional, Zhou, Zhi, additional, Wang, Yun, additional, Kong, Lingsheng, additional, Zhong, Ning, additional, Chai, Renjie, additional, Luo, Xiangfeng, additional, Guo, Yike, additional, Hawrylycz, Michael, additional, Luo, Qingming, additional, Gu, Zhongze, additional, Xie, Wei, additional, Zeng, Hongkui, additional, and Peng, Hanchuan, additional

Published
2019
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225. Wnt Signaling Activates TP53-Induced Glycolysis and Apoptosis Regulator and Protects Against Cisplatin-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea

Author

Liu, Wenwen, primary, Xu, Xiaochen, additional, Fan, Zhaomin, additional, Sun, Gaoying, additional, Han, Yuechen, additional, Zhang, Daogong, additional, Xu, Lei, additional, Wang, Mingming, additional, Wang, Xue, additional, Zhang, Shasha, additional, Tang, Mingliang, additional, Li, Jianfeng, additional, Chai, Renjie, additional, and Wang, Haibo, additional

Published
2019
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226. Critical role of spectrin in hearing development and deafness

Author

Liu, Yan, primary, Qi, Jieyu, additional, Chen, Xin, additional, Tang, Mingliang, additional, Chu, Cenfeng, additional, Zhu, Weijie, additional, Li, Hui, additional, Tian, Cuiping, additional, Yang, Guang, additional, Zhong, Chao, additional, Zhang, Ying, additional, Ni, Guangjian, additional, He, Shuijin, additional, Chai, Renjie, additional, and Zhong, Guisheng, additional

Published
2019
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227. Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function

Author

Fang, Qiaojun, primary, Zhang, Yuhua, additional, Da, Peng, additional, Shao, Buwei, additional, Pan, Haolai, additional, He, Zuhong, additional, Cheng, Cheng, additional, Li, Dan, additional, Guo, Jiaqi, additional, Wu, Xiaohan, additional, Guan, Ming, additional, Liao, Menghui, additional, Zhang, Yuan, additional, Sha, Suhua, additional, Zhou, Zikai, additional, Wang, Jian, additional, Wang, Tian, additional, Su, Kaiming, additional, Chai, Renjie, additional, and Chen, Fangyi, additional

Published
2019
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231. The role of FOXG1 in the postnatal development and survival of mouse cochlear hair cells

Author

He, Zuhong, primary, Fang, Qiaojun, additional, Li, He, additional, Shao, Buwei, additional, Zhang, Yuan, additional, Zhang, Yuhua, additional, Han, Xiao, additional, Guo, Rongrong, additional, Cheng, Cheng, additional, Guo, Lingna, additional, Shi, Lusen, additional, Li, Ao, additional, Yu, Chenjie, additional, Kong, Weijia, additional, Zhao, Chunjie, additional, Gao, Xia, additional, and Chai, Renjie, additional

Published
2019
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232. Gene editing in a Myo6semi-dominant mouse model rescues auditory function

Author

Xue, Yuanyuan, Hu, Xinde, Wang, Daqi, Li, Di, Li, Yige, Wang, Fang, Huang, Mingqian, Gu, Xi, Xu, Zhijiao, Zhou, Jinan, Wang, Jinghan, Chai, Renjie, Shen, Jun, Chen, Zheng-Yi, Li, Geng-Lin, Yang, Hui, Li, Huawei, Zuo, Erwei, and Shu, Yilai

Abstract

Myosin VI（MYO6） is an unconventional myosin that is vital for auditory and vestibular function. Pathogenic variants in the human MYO6gene cause autosomal-dominant or -recessive forms of hearing loss. Effective treatments for Myo6mutation causing hearing loss are limited. We studied whether adeno-associated virus (AAV)-PHP.eB vector-mediated in vivodelivery of Staphylococcus aureusCas9 (SaCas9-KKH)-single-guide RNA (sgRNA) complexes could ameliorate hearing loss in a Myo6WT/C442Ymouse model that recapitulated the phenotypes of human patients. The in vivoediting efficiency of the AAV-SaCas9-KKH-Myo6-g2 system on Myo6C442Yis 4.05% on average in Myo6WT/C442Ymice, which was ∼17-fold greater than editing efficiency of Myo6WTalleles. Rescue of auditory function was observed up to 5 months post AAV-SaCas9-KKH-Myo6-g2 injection in Myo6WT/C442Ymice. Meanwhile, shorter latencies of auditory brainstem response (ABR) wave I, lower distortion product otoacoustic emission (DPOAE) thresholds, increased cell survival rates, more regular hair bundle morphology, and recovery of inward calcium levels were also observed in the AAV-SaCas9-KKH-Myo6-g2-treated ears compared to untreated ears. These findings provide further reference for in vivogenome editing as a therapeutic treatment for various semi-dominant forms of hearing loss and other semi-dominant diseases.

Published
2022
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236. Bio-inspired intestinal scavenger from microfluidic electrospray for detoxifying lipopolysaccharide

Author

Zhao, Cheng, Chen, Guopu, Wang, Huan, Zhao, Yuanjin, and Chai, Renjie

Abstract

Lipopolysaccharide (LPS) plays an important role in metabolic syndrome (MetS) and other gut-derived diseases, and detoxifying LPS is considered to be a fundamental approach to prevent and treat these diseases. Here, inspired by the feeding behaviour of scavenger, novel microfluidic microcapsules with alkaline phosphatase (ALP) encapsulation and the scavenger-like molecular sieve shell are presented for cleaning intestinal LPS. Benefiting from the precisely controlled of the pore size and microfluidic electrospray, the generated microcapsules were imparted with porous molecular-sieve shells and ALP encapsulated active cores. These microcapsules could continuously work as an intestinal scavenger after colonized in intestine. It has been demonstrated that the microcapsules could englobe LPS while inhibit the permeation of digestive enzyme, and this ability contributes to promising ALP's activity, protecting cells at the presence of LPS and reducing inflammation. In addition, this scavenger inspired microcapsule could effectively decrease the LPS in organs, reduce inflammation and regulating fat metabolism in vivo. These features make the ALP encapsulated microcapsules an ideal candidate for treating MetS and other LPS related diseases.

Published
2021
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237. Knockdown of Foxg1 in supporting cells increases the trans-differentiation of supporting cells into hair cells in the neonatal mouse cochlea.

Author

Zhang, Shasha, Zhang, Yuan, Dong, Ying, Guo, Lingna, Zhang, Zhong, Shao, Buwei, Qi, Jieyu, Zhou, Han, Zhu, Weijie, Yan, Xiaoqian, Hong, Guodong, Zhang, Liyan, Zhang, Xiaoli, Tang, Mingliang, Zhao, Chunjie, Gao, Xia, and Chai, Renjie

Subjects
HAIR cells, INNER ear, CELL determination, NOTCH signaling pathway, COCHLEA, CELL cycle, MICE
Abstract

Foxg1 is one of the forkhead box genes that are involved in morphogenesis, cell fate determination, and proliferation, and Foxg1 was previously reported to be required for morphogenesis of the mammalian inner ear. However, Foxg1 knock-out mice die at birth, and thus the role of Foxg1 in regulating hair cell (HC) regeneration after birth remains unclear. Here we used Sox2CreER/+ Foxg1loxp/loxp mice and Lgr5-EGFPCreER/+ Foxg1loxp/loxp mice to conditionally knock down Foxg1 specifically in Sox2+ SCs and Lgr5+ progenitors, respectively, in neonatal mice. We found that Foxg1 conditional knockdown (cKD) in Sox2+ SCs and Lgr5+ progenitors at postnatal day (P)1 both led to large numbers of extra HCs, especially extra inner HCs (IHCs) at P7, and these extra IHCs with normal hair bundles and synapses could survive at least to P30. The EdU assay failed to detect any EdU+ SCs, while the SC number was significantly decreased in Foxg1 cKD mice, and lineage tracing data showed that much more tdTomato+ HCs originated from Sox2+ SCs in Foxg1 cKD mice compared to the control mice. Moreover, the sphere-forming assay showed that Foxg1 cKD in Lgr5+ progenitors did not significantly change their sphere-forming ability. All these results suggest that Foxg1 cKD promotes HC regeneration and leads to large numbers of extra HCs probably by inducing direct trans-differentiation of SCs and progenitors to HCs. Real-time qPCR showed that cell cycle and Notch signaling pathways were significantly down-regulated in Foxg1 cKD mice cochlear SCs. Together, this study provides new evidence for the role of Foxg1 in regulating HC regeneration from SCs and progenitors in the neonatal mouse cochlea. [ABSTRACT FROM AUTHOR]

Published
2020
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238. Increased mitochondrial DNA copy number protects hair cells and HEI-OC1 cells against drug-induced apoptosis.

Author

Mei, Honglin, Mei, Dongmei, Yu, Huiqian, Sun, Shan, Chen, Yan, Zhang, Yanping, Chai, Renjie, and Li, Huawei

Subjects
HAIR cells, MITOCHONDRIAL DNA, DNA, MITOCHONDRIAL membranes, CELLS, CELL physiology, MEMBRANE permeability (Biology)
Abstract

Several factors trigger apoptosis in cochlear hair cells. Previous studies have shown that mitochondria play key roles in apoptosis, but the role of mitochondrial deoxyribonucleic acid (mtDNA) copy number in the pathogenesis of hair cell apoptosis remains largely unknown. We used mouse cochlear hair cells and House Ear Institute-Organ of Corti 1 (HEI-OC1) cells to explore the relationship between mtDNA copy number and cell apoptosis. We found that the mtDNA copy number of hair cells was reduced relative to mitochondrial mass and hypothesized that increasing it might have a protective effect. We then increased the mtDNA copy number of the hair and HEI-OC1 cells by transfecting them with an adeno-associated virus (AAV) vector containing mitochondrial transcription factor A (TFAM). We found that the apoptosis rates decreased upon inducing apoptosis with neomycin or cisplatin (DDP). To elucidate the mechanisms, we analyzed the mitochondrial-membrane permeability and mitochondrial function of HEI-OC1 cells. Our results suggested that the increase in mtDNA copy number could protect hair cells and HEI-OC1 cells against drug-induced apoptosis by stabilizing the permeability of the mitochondrial membrane and mitochondrial function. [ABSTRACT FROM AUTHOR]

Published
2020
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239. Loss of ARHGEF6 Causes Hair Cell Stereocilia Deficits and Hearing Loss in Mice

Author

Zhu, Chengwen, primary, Cheng, Cheng, additional, Wang, Yanfei, additional, Muhammad, Waqas, additional, Liu, Shuang, additional, Zhu, Weijie, additional, Shao, Buwei, additional, Zhang, Zhong, additional, Yan, Xiaoqian, additional, He, Qingqing, additional, Xu, Zhengrong, additional, Yu, Chenjie, additional, Qian, Xiaoyun, additional, Lu, Ling, additional, Zhang, Shasha, additional, Zhang, Yuan, additional, Xiong, Wei, additional, Gao, Xia, additional, Xu, Zhigang, additional, and Chai, Renjie, additional

Published
2018
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240. Hoxc-Dependent Mesenchymal Niche Heterogeneity Drives Regional Hair Follicle Regeneration

Author

Yu, Zhou, primary, Jiang, Kaiju, additional, Xu, Zijian, additional, Huang, Huanwei, additional, Qian, Nannan, additional, Lu, Zhiwei, additional, Chen, Daoming, additional, Di, Ruonan, additional, Yuan, Tianyi, additional, Du, Zhenhai, additional, Xie, Wei, additional, Lu, Xiaoling, additional, Li, Huawei, additional, Chai, Renjie, additional, Yang, Yong, additional, Zhu, Bing, additional, Kunieda, Tetsuo, additional, Wang, Fengchao, additional, and Chen, Ting, additional

Published
2018
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243. Meclofenamic Acid Reduces Reactive Oxygen Species Accumulation and Apoptosis, Inhibits Excessive Autophagy, and Protects Hair Cell-Like HEI-OC1 Cells From Cisplatin-Induced Damage

Author

Li, He, primary, Song, Yongdong, additional, He, Zuhong, additional, Chen, Xiaoyun, additional, Wu, Xianmin, additional, Li, Xiaofei, additional, Bai, Xiaohui, additional, Liu, Wenwen, additional, Li, Boqin, additional, Wang, Shanshan, additional, Han, Yuechen, additional, Xu, Lei, additional, Zhang, Daogong, additional, Li, Jianfeng, additional, Chai, Renjie, additional, Wang, Haibo, additional, and Fan, Zhaomin, additional

Published
2018
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244. Characterization of Lgr6+ Cells as an Enriched Population of Hair Cell Progenitors Compared to Lgr5+ Cells for Hair Cell Generation in the Neonatal Mouse Cochlea

Author

Zhang, Yanping, primary, Guo, Luo, additional, Lu, Xiaoling, additional, Cheng, Cheng, additional, Sun, Shan, additional, Li, Wen, additional, Zhao, Liping, additional, Lai, Chuijin, additional, Zhang, Shasha, additional, Yu, Chenjie, additional, Tang, Mingliang, additional, Chen, Yan, additional, Chai, Renjie, additional, and Li, Huawei, additional

Published
2018
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250. Hedgehog Signaling Promotes the Proliferation and Subsequent Hair Cell Formation of Progenitor Cells in the Neonatal Mouse Cochlea

Author

Chen, Yan, primary, Lu, Xiaoling, additional, Guo, Luo, additional, Ni, Wenli, additional, Zhang, Yanping, additional, Zhao, Liping, additional, Wu, Lingjie, additional, Sun, Shan, additional, Zhang, Shasha, additional, Tang, Mingliang, additional, Li, Wenyan, additional, Chai, Renjie, additional, and Li, Huawei, additional

Published
2017
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