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El objetivo de este artículo es provocar un debate sobre el futuro de la universidad pública y su modelo de organización. El modelo actual de universidad parece estar en crisis. De los varios factores que contribuyen a esta afirmación, este artículo centra su atención en la idiosincrasia de su modelo organizativo y en las dificultades que éste plantea a la hora de amoldarse a los nuevos retos sociales. Por ello, en los últimos tiempos han aumentado las demandas externas reclamando mayores controles burocráticos que garanticen mayor eficacia en los logros docentes e investigadores. Sin embargo, la consolidación de este modelo supondría sacrificar la capacidad innovadora y de respuesta académica en pos de modelos de trabajo que fomentan menor audacia y mayor uniformidad. A este respecto, se ofrece una propuesta de reformulación de los principios organizativos académicos orientándolos a un modelo de universidad permeable a las necesidades de la sociedad y los diversos grupos de interés a través de la puesta en marcha de prácticas organizativas colaborativas.

Many physics faculty are aware of and interested in using research-based instructional strategies. However, knowledge and motivation are not sufficient to support successful and sustained adoption. To address this problem, we present a faculty online learning community (FOLC) model for educational change and describe its application (the New Faculty Workshop FOLC, NFW-FOLC) to provide a year of support to new faculty following attendance at the Physics and Astronomy New Faculty Workshop. FOLCs are an extension of the faculty learning community model. Here we present the theoretical underpinnings of the general FOLC model, the design principles of our particular NFW-FOLCs, and the learning objectives for NFW-FOLC participants. We demonstrate the efficacy of our NFW-FOLCs by analyzing postexperience interviews with participants from the first four NFW-FOLC cohorts. The primary motivation given by faculty for joining our FOLCs is to improve their teaching as they learn more about teaching strategies, get implementation help, and connect to a broader community. These motivations align with our NFW-FOLC design principles. Participants overwhelmingly report the experience was valuable and impacted them positively with the most commonly reported impacts of NFW-FOLC membership being changes in their implementation of teaching strategies, increased reflection about teaching, increased confidence as teachers, increased knowledge about teaching, benefits to their students, time saved, and gaining a resource. The reported impacts provide evidence that the NFW-FOLC is successfully meeting its goals. We argue that the reported impacts are uniquely supported by the FOLC model of professional development. We advance FOLCs as a generalizable model of professional development offering a number of advantages over traditional reform efforts as well as traditional Faculty Learning Communities.

Electronic money (e-money or e-Cash) is the digital representation of physical banknotes augmented by added use cases of online and remote payments. This paper presents a novel, anonymous e-money transaction protocol, built based on physical unclonable functions (PUFs), titled PUF-Cash. PUF-Cash preserves user anonymity while enabling both offline and online transaction capability. The PUF’s privacy-preserving property is leveraged to create blinded tokens for transaction anonymity while its hardware-based challenge−response pair authentication scheme provides a secure solution that is impervious to typical protocol attacks. The scheme is inspired from Chaum’s Digicash work in the 1980s and subsequent improvements. Unlike Chaum’s scheme, which relies on Rivest, Shamir and Adlemans’s (RSA’s) multiplicative homomorphic property to provide anonymity, the anonymity scheme proposed in this paper leverages the random and unique statistical properties of synthesized integrated circuits. PUF-Cash is implemented and demonstrated using a set of Xilinx Zynq Field Programmable Gate Arrays (FPGAs). Experimental results suggest that the hardware footprint of the solution is small, and the transaction rate is suitable for large-scale applications. An in-depth security analysis suggests that the solution possesses excellent statistical qualities in the generated authentication and encryption keys, and it is robust against a variety of attack vectors including model-building, impersonation, and side-channel variants.

Obscure gastrointestinal bleeding (OGIB) can present as a diagnostic dilemma and management can be challenging. The search for causes of OGIB is usually centered on visualizing the small bowel, and in the past decade, the technology to visualize the entire small bowel has significantly advanced. Moreover, small bowel endoscopic imaging has replaced, in many instances, prior radiographic evaluation for obscure GI bleeding. These new modalities, such as small bowel capsule endoscopy (CE), balloon-assisted deep enteroscopy [double balloon enteroscopy (DBE) and single balloon enteroscopy (SBE)], and overtube-assisted deep enteroscopy (spiral enteroscopy), are paving the way toward more accurately identifying and treating patients with OGIB. We will review the diagnostic modalities available in evaluating a patient with OGIB and also propose the management based on clinical and endoscopic findings.

School transmission of an intelligibility of the past is subject to all kinds of pressure in the public space, and even a real tyrannical orthodoxy. Countless actors proclaim a sententious tone that should be school history, usually in a straitjacket of identity and prescriptive far from any scientific requirement. But this is for the school context, the didactic transposition of a social science discipline whose purpose is to promote a self-critical thought and discernment to act in the social world. This objective requires giving up both to a fall on a closed and inculcation of cumulative knowledge and education is sententious, is so entertaining it would not allow students to work this feeling of strangeness that should mark any relationship past and its differences. It is however possible to make them tasty build knowledge that enable them to better understand the world in which they live.

Chronic Budd-Chiari syndrome can present with cirrhosis and signs and symptoms similar to those of other chronic liver diseases. We present a case of Budd-Chiari syndrome discovered during attempted transjugular intrahepatic portosystemic shunting in a patient with decompensated cirrhosis believed to be secondary to hepatitis C. Although the patient had hepatocellular carcinoma, the Budd-Chiari syndrome was a primary disease due to hepatic venous webs. Angioplasty was performed in this case, which resolved the patient’s symptoms related to portal hypertension. Follow-up venography 5 months after angioplasty demonstrated continued patency of the hepatic veins. A biopsy was obtained in the same setting, which showed centrilobular fibrosis indicating that venous occlusion was indeed the cause of cirrhosis. It is important to consider a second disease when treating a patient with difficult to manage portal hypertension.

Abstract The purpose of this study was to investigate the relationship between Sense of Coherence (SOC) and oral health clinical variables (number of teeth present and absence of need for dental prostheses). The sample consisted of 720 adults and elderly Brazilians. The data were collected at home using the SOC-13 scale – a form of clinical examination for the evaluation of oral conditions – and a questionnaire evaluating socioeconomic aspects and the use of dental services. Statistical analysis, both univariate and multivariate, was performed by Poisson regression with robust variance adjustment. The average age of the participants was 60.2 years, and they were predominantly female (57.8%). Among the participants who had a strong SOC, the absence of the need for dental prostheses was 34% higher than among those with a weak SOC, demonstrating a significant difference between the groups (PR = 1.34, 95%CI = 1.06–1.70; p = 0.015). Individuals who had a strong SOC had a 5% higher prevalence of 14 or more existing teeth than those with a weak SOC, which was statistically significant after adjustment for co-factors (PR = 1.05, 95%CI = 1.01–1.11, p = 0.033). A strong personal Sense of Coherence has a beneficial influence on the oral health of adults and older people in Brazil.

Objective: To analyze the structure of prenatal assistance and pharmaceutical service in ten family health centers in the municipality of Fortaleza - CE, Brazil. Methods: A descriptive study with quantitative approach, conducted in the period from January to December, 2009. The studied variables were: health center and attendance room structure conditions; available materials and equipments; tools for registration and follow up of activities; human resources; local pharmaceutical assistance management; medicine storage and distribution. Data collection consisted of a systematic observation of the structure of the prenatal care in the centers and of a form applied to pharmacists about the pharmacy service management. The variables related to the structure of the family health centers and of the pharmacy services were analyzed and measured by scale: insufficient (

Objective: To analyze the structure of prenatal assistance and pharmaceutical service in ten family health centers in the municipality of Fortaleza - CE, Brazil. Methods: A descriptivestudy with quantitative approach, conducted in the period from January to December, 2009. The studied variables were: health center and attendance room structure conditions; available materials and equipments; tools for registration and follow up of activities;human resources; local pharmaceutical assistance management; medicine storage and distribution. Data collection consisted of a systematic observation of the structure of the prenatal care in the centers and of a form applied to pharmacists about the pharmacy servicemanagement. The variables related to the structure of the family health centers and of the pharmacy services were analyzed and measured by scale: insufficient (

Objective: To investigate the pattern of Non-obstetrical genital fistula (NOGF) in Cameroonian context. Materials and methods: This was a cross-sectional analytic study, with two groups of patients, including those treated for non-obstetrical fistula as subject, and their obstetrical counterparts as the control. Data were retrieved from the obstetric fistula database of the Department of Obstetrics and Gynecology in the University Teaching Hospital of Yaoundé Cameroon for patients operated from January, 1, 2009 to December 31, 2012. Results: NOGF represented 19.78% of genital fistula. Uro-genital fistula (UGF) represented 72.20% while non-obstetrical genito-digestive fistula (GDF) represented 27.80% of NOGF. The main cause of UGF was hysterectomy (46.15%) while the main cause of genito-digestive fistula was vaginal infections (40.00%). Most patients had never been operated before their arrival in the YUTH (84.60% for UGF and 60.00% for GDF). We had one failure at first surgical attempt. Conclusion: UGF fistula is the main type of NOGF in Cameroonian context, with hysterectomy being the leading cause. Proper knowledge on NOGF will enable better strategies to fight against genital fistula.

The paper investigated the residual strain and stress distribution, microstructure, and macro-texture along the transverse direction of commercially pure titanium grade 2 samples prepared by the CONFORM ECAP technique. This method belongs to the severe plastic deformation methods; hence, it could be assumed that residual stress fields would be present in the work-pieces. Residual stresses cannot be directly measured; thus, neutron diffraction measurements, Electron back-scatter diffraction (EBSD) investigations, and local X-ray macro-texture measurements were performed in different regions of the sample to determine the data for the residual stress calculation. The calculation was based on the modified Kröner model. Neutron diffraction strain scans and residual stress calculations revealed that symmetrical residual strain and stress gradients with compression character were present in the axial and hoop direction after one and two passes. Asymmetric distribution of the residual strains and stresses remained after the third pass of the CONFORM ECAP. EBSD investigations showed that after the first pass, significant grain refinement occurred; however, further passes did not cause any dramatic grain refinement. X-ray texture measurements revealed that local macro-texture was dependent on the number of passes of the CONFORM ECAP and on the investigated area in the samples.

Heparan sulfate 3-O-sulfotransferases (HS3STs) catalyze the maturation step of heparan sulfate (HS) 3-O-sulfation. This modification is relatively rare. Moreover, only a few biological processes have been described to be influenced by 3-O-sulfated HS, and few ligands have been identified so far. Among them, neuropilin-1 (Nrp1) was reported to exhibit tumor-promoting properties by enhancing the action of various growth factors. We recently demonstrated that transient overexpression of HS3ST2, 3B or 4 enhanced the proliferation of breast cancer MDA-MB-231 cells and promote efficient protection against pro-apoptotic stimuli. Hence, we hypothesized that the pro-tumoral activity of these HS3STs could depend on the expression of Nrp1. To test this, MDA-MB-231 cells were stably transfected with a construct encoding HS3ST3B and the expression of Nrp1 was down-regulated by RNA interference. First, we confirmed that stable expression of HS3ST3B effectively increased cell proliferation and viability. Silencing the expression of Nrp1 markedly attenuated the promoting effects of HS3ST3B, while the same treatment had only a moderate effect on the behavior of the parental cells. Altogether, our findings support the idea that the tumor-promoting effects of HS3ST3B could be dependent on the expression of Nrp1 in cancer cells.

Until now there have been no reliable historical or skeletal fossil records for the giraffe (Giraffa camelopardalis) south of the Orange River or northern Namaqualand. The recent discovery of fossil giraffe tracks in coastal aeolianites east of Still Bay, South Africa, significantly increases the geographical range for this species, and has implications for Late Pleistocene climate and vegetation in the southern Cape. Giraffe populations have specialised needs, and require a savanna ecosystem. Marine geophysical and geological evidence suggests that the broad, currently submerged floodplains of the Gouritz and Breede Rivers likely supported a productive savanna of Vachellia karroo during Pleistocene glacial conditions, which would have provided a suitable habitat for this species. We show evidence for the hypothesis that the opening of the submerged shelf during glacial periods acted as a pathway for mammals to migrate along the southern coastal plain. Significance: • The identification of fossil giraffe tracks on the Cape south coast, far from the area in which giraffe have previously been known to occur, is unexpected; conclusions about prehistoric conditions and vegetation can be drawn from this discovery.

Natasha Trethewey welcomes the 2014 Callaloo Conference, "Making Art: Writing, Authorship, and Critique," and Charles Henry Rowell describes the significance of this scholarly gathering hosted at Emory University.

Aging is a multifactorial process that includes the lifelong accumulation of molecular damage, leading to age-related frailty, disability and disease, and eventually death. In this study, we report evidence of a significant correlation between the number of genes encoding the immunomodulatory CD33-related sialic acid-binding immunoglobulin-like receptors (CD33rSiglecs) and maximum lifespan in mammals. In keeping with this, we show that mice lacking Siglec-E, the main member of the CD33rSiglec family, exhibit reduced survival. Removal of Siglec-E causes the development of exaggerated signs of aging at the molecular, structural, and cognitive level. We found that accelerated aging was related both to an unbalanced ROS metabolism, and to a secondary impairment in detoxification of reactive molecules, ultimately leading to increased damage to cellular DNA, proteins, and lipids. Taken together, our data suggest that CD33rSiglecs co-evolved in mammals to achieve a better management of oxidative stress during inflammation, which in turn reduces molecular damage and extends lifespan.

Objectives: Recent reports have highlighted the benefits of surgical division of tongue-tie (frenulotomy) in infants with breastfeeding difficulties. There is no clear consensus defining the appropriate age for this procedure to be undertaken in selected infants. We aimed to evaluate the impact of delays in time between referral and frenulotomy in relation to maternal abandonment of breastfeeding. Materials and Methods: This was a prospective cohort study done in out-patient Neonatal Surgery Department, Birmingham Heartlands Hospital, Birmingham, UK, between April 2013 and July 2013. All infants, referred to our tongue-tie clinic between April and July 2013, were studied prospectively. Referral time lags were calculated using computer records; details regarding breastfeeding were collected by an independent interviewer completing a questionnaire. Results: Seventy patients were included. The median infant age at clinic was 28.5 days [range 1-126]. Fifty eight [82%] of mothers had breastfeeding difficulty and their infants were confirmed to have a prominent tongue-tie. By the time of their clinic attendance, breastfeeding had either not been established or abandoned in 21%. Despite difficulty, 61% of mothers persisted breastfeeding and all these mothers consented for frenulotomy. At time of clinic, median age of infants whose mothers had abandoned breastfeeding was 37 days [range 1-80] compared to 27 days [range 1-126] in infants whose mothers had persisted. Conclusions: We demonstrated a time-critical dimension for frenulotomy: delay beyond 4-weeks from referral to assessment of neonatal tongue-tie is more likely to be associated with abandonment of breastfeeding. Timely assessment and division of tongue-tie in selected infants can therefore play an important role in a birthing unit’s breastfeeding strategy.

Post-tsunami field surveys of the Maldives Islands where carried out to document the effects of the tsunami inundation. The study area was situated in the islands of South Male Atoll that were some of the most heavily damaged islands of the Maldive Islands. The tsunami damaged the natural environment, vegetation, man-made structures, and residents. The maximum tsunami wave height was 3-4 m. This level of inundation exceeded the height of most residents. The wave height was greatest on the eastern rim of the South Male Atoll (closest to the tsunami source) and these islands were completely flooded. The islands within the interior of the atoll saw the lowest wave heights, and these were only marginally flooded.Surveys of flood lines left on the exterior and interior of structures were measured but proved to be substantially less than that reported by survivors. It appears that the highest inundation was not preserved as flood lines. We suggest that the turbulence associated with the tsunami inundation erased the highest lines or that they did not form due to an absence of debris and organic compounds that acted as adhesion during the initial flooding.Significant erosion was documented. Deposition took place in the form of sand sheets while only desultory deposition of coral clasts in marginal areas was found. Seasonal erosion, and storms are likely to remove most or all of the traces of the tsunami within these islands.

Control of West Nile virus (WNV) can only be effective if the vectors and reservoirs of the virus are identified and controlled. Although mosquitoes are the primary vectors, WNV has repeatedly been isolated from ticks. Therefore tick-borne transmission studies were performed with an ixodid (Ixodes ricinus) and an argasid tick species (Ornithodoros moubata). Both species became infected after feeding upon viremic hosts, but I. ricinus ticks were unable to maintain the virus. In contrast, O. moubata ticks were infected for at least 132 days, and the infection was maintained through molting and a second bloodmeal. Infected O. moubata ticks transmitted the virus to rodent hosts, albeit at a low level. Moreover, the virus was nonsystemically transmitted between infected and uninfected O. moubata ticks co-fed upon uninfected hosts. Although ticks are unlikely to play a major role in WNV transmission, our findings suggest that some species have the potential to act as reservoirs for the virus.

A pesca na Amazônia representa uma importante atividade social, econômica e cultural, com desembarque de pescado estimado em 25 mil toneladas por ano, somente no terminal de desembarque de Manaus, porém com poucas avaliações socioeconômicas que são fundamentais para o manejo da atividade. O presente trabalho visou avaliar aspectos funcionais das operações de pesca sob o ponto de vista econômico. As informações foram coletadas por intermédio de questionário estruturado junto aos proprietários das embarcações e, ou encarregados das expedições de pesca, entre outubro de 1999 e junho de 2000. Foram amostradas 92 embarcações, para destacar os aspectos referentes aos custos efetuados quando das expedições de pesca. Embarcações pequenas apresentaram custo operacional médio por expedição de 45,2% da renda bruta da venda do pescado, embarcações médias e grandes apresentaram valores de 42,0% e 47,8% dos custos respectivamente. Os combustíveis se destacaram como o componente principal destes custos.Fishery in the Amazon represents an important social, economic and cultural activity with yearly fish landings around 25 thousand tons in the fishing harbour of Manaus alone, with little socioeconomic evaluations that are fundamental for its management. The present paper sought to evaluate functional aspects of fishing operations from the economic point of view. Information was collected from 92 boats, between October of 1999 and June of 2000, through questionnaires given to boat owners or general managers of fishing expeditions. Small fishing boats presented mean operational cost of 45.2% of the gross income of fish sales. Medium and larger boats presented values of 42.0% and 47.8% costs, respectively, where fuel was the main expenditure.

OBJECTIVE: health promotion and disease prevention activities directed to osteoporosis might help to reduce the rate of osteoporotic fractures among elderly people. METHODS: in order to check whether osteoporotic women modify their habits after the fracture, 518 postmenopausal white Brazilian elderly women were recruited from the outpatient clinic of the Rheumatology Division (122 of them with fracture) and were followed for one year. Questionnaire of evaluation was based on the European Vertebral Osteoporosis Study (EVOS) and inquiries about topics related to falls, bone mass and fracture. Lateral thoracic and lumbar radiographs were taken according to a standard protocol in order to verify vertebral fracture. Bone mineral density was measured using a bone densitometer (Lunar DPX, Madison, WI). Women's behavior was analyzed before and after the fracture. RESULTS: before the fracture, 34% of them had poor health perception, 40.2% walked at least half an hour per day, 14.7% used canes, 56.6% complained of dizziness, 59.6% scattered rugs, 78.9% used public transportation, 21.1% used car, and 36.8% wore leather instead of rubber sole. After the fracture, 66.4% of those women had worse health perception; 69.7% became more sedentary, 27.9% used more canes, 63.4% complained of more dizziness, 38.3% removed rugs, 68.1% changed from public to private car transportation, and 55.7% modified their shoes from leather to rubber sole. Risk factors related to bone mass did not change before and after the fractures. CONCLUSIONS: these findings suggested that women modify only lifestyle habits related to falls but not those related to bone mass after osteoporotic fracture. Further research is needed in order to check which intervention strategies may lead to better results in preventing osteoporotic fractures.OBJETIVO: atividades de promoção de saúde e prevenção direcionadas para osteoporose podem ajudar a reduzir a taxa de fraturas osteoporóticas na população idosa. MÉTODOS: para avaliar se mulheres com osteoporose modificam seus hábitos de vida após a fratura, selecionamos 518 mulheres idosas caucasianas de nosso Serviço Ambulatorial de Reumatologia (122 com fratura), que foram seguidas por um ano. O questionário de avaliação foi baseado no European Vertebral Osteoporosis Study (EVOS) e contém tópicos relacionados com queda, massa óssea e fratura. Radiografias lateral da coluna torácica e lombar foram realizadas de acordo com protocolo padrão para verificar fratura vertebral. Densidade mineral óssea foi medida por meio de densitômetro Lunar DPX, Madison, WI. O comportamento das mulheres foi analisado antes e após a fratura. RESULTADOS: antes da fratura, 34% das mulheres tinham pior percepção da saúde, 40,2% caminhavam pelo menos meia hora por dia, 14,7% usavam bengalas, 56,6% queixavam-se de tonturas, 59,6% possuíam tapetes espalhados pela casa, 78,9% usavam transporte público, 21,1% utilizavam transporte privado (carro) e 36,8% usavam solado de couro e não de borracha. Após a fratura, 66,4% das mulheres tinham pior percepção da saúde; 69,7% ficaram mais sedentárias, 27,9% usavam mais bengalas, 63,4% queixavam-se de mais tonturas, 38,3% tinham retirado os tapetes de casa, 68,1% trocaram o transporte público por privado e 55,7% modificaram o solado dos sapatos de couro para borracha. Fatores de risco relacionados com a massa óssea não foram modificados após a fratura. CONCLUSÕES: estes achados sugerem que mulheres idosas modificam somente os hábitos de vida relacionados com quedas, mas não aqueles relacionados com a massa óssea após a fratura por osteoporose. Pesquisas são necessárias a fim de avaliar quais estratégias de intervenção podem ocasionar melhores resultados na prevenção de fraturas por osteoporose.

Herder et al. (IEEE Transactions on Dependable and Secure Computing, 2017) designed a new computational fuzzy extractor and physical unclonable function (PUF) challenge-response protocol based on the Learning Parity with Noise (LPN) problem. The protocol requires no irreversible state updates on the PUFs for security, like burning irreversible fuses, and can correct for significant measurement noise when compared to PUFs using a conventional (information theoretical secure) fuzzy extractor. However, Herder et al. did not implement their protocol. In this paper, we give the first implementation of a challenge response protocol based on computational fuzzy extractors. Our main insight is that “confidence information” does not need to be kept private, if the noise vector is independent of the confidence information, e.g., the bits generated by ring oscillator pairs which are physically placed close to each other. This leads to a construction which is a simplified version of the design of Herder et al. (also building on a ring oscillator PUF). Our simplifications allow for a dramatic reduction in area by making a mild security assumption on ring oscillator physical obfuscated key output bits.

At large research universities, physics graduate teaching assistants (TAs) are often responsible for grading in courses at all levels. However, few studies have focused on TAs’ grading practices in introductory and advanced physics courses. This study was designed to investigate whether physics graduate TAs grade students in introductory physics and quantum mechanics using different criteria and if so, why they may be inclined to do so. To investigate possible discrepancies in TAs’ grading approaches in courses at different levels, we implemented a sequence of instructional activities in a TA professional development course that asked TAs to grade student solutions of introductory physics and upper-level quantum mechanics problems and explain why, if at all, their grading approaches were different or similar in the two contexts. We analyzed the differences in TAs’ grading approaches in the two contexts and discuss the reasons they provided for the differences in their grading approaches in introductory physics and quantum mechanics in individual interviews, class discussions, and written responses. We find that a majority of the TAs graded solutions to quantum mechanics problems differently than solutions to introductory physics problems. In quantum mechanics, the TAs focused more on physics concepts and reasoning and penalized students for not showing evidence of understanding. The findings of the study have implications for TA professional development programs, e.g., the importance of helping TAs think about the difficulty of a problem from an introductory students’ perspective and reflecting on the benefits of formative assessment.

Risk assessment of tick-borne and zoonotic disease emergence necessitates sound knowledge of the particular microorganisms circulating within the communities of these major vectors. Assessment of pathogens carried by wild ticks must be performed without a priori, to allow for the detection of new or unexpected agents.We evaluated the potential of Next-Generation Sequencing techniques (NGS) to produce an inventory of parasites carried by questing ticks. Sequences corresponding to parasites from two distinct genera were recovered in Ixodes ricinus ticks collected in Eastern France: Babesia spp. and Theileria spp. Four Babesia species were identified, three of which were zoonotic: B. divergens, Babesia sp. EU1 and B. microti; and one which infects cattle, B. major. This is the first time that these last two species have been identified in France. This approach also identified new sequences corresponding to as-yet unknown organisms similar to tropical Theileria species.Our findings demonstrate the capability of NGS to produce an inventory of live tick-borne parasites, which could potentially be transmitted by the ticks, and uncovers unexpected parasites in Western Europe.

BACKGROUND AND AIMS: Ticks are highly susceptible to global environmental and socio-economical changes. Several tick-borne pathogens have been reported in new geographical regions while new species, strains or genetic variants of tick-borne microorganisms are continually being detected. However, tick-borne pathogens are still poorly understood, and it is estimated that half of all human tick-borne disease has an unknown origin. Therefore in order to prevent these diseases, more effort is required to identify unknown or unexpected tick-borne pathogens. Ixodes ricinus is the vector for a broad range of bacterial pathogens and the most prevalent tick in Europe. The aim of the present study was to evaluate the capability of Next Generation Sequencing (NGS) to extend the inventory of pathogenic bacteria carried by this species of tick in France. METHODS: RNA and DNA were extracted from 1450 I. ricinus questing nymphs collected by flagging in Alsace, France. RNA was pooled and used for NGS. Following de novo assembly, bacterial contigs were assigned to the closest known taxonomy. DNA was used for real time PCR to confirm taxonomic species assignment of NGS-derived contigs for the doubtful cases, and for determination of prevalence. RESULTS: We have generated a global in-depth picture of tick-borne bacteria. We identified RNA from the main pathogenic bacterial species known to be transmitted by I. ricinus. In addition we also identified unanticipated bacterial species for which we have estimated the prevalence within those ticks inhabiting the studied areas. CONCLUSIONS: The data obtained from this study has proven that NGS has an enormous potential to detect the unexpected and provides the means to monitor pathogen occurrence.

[This paper is part of the Focused Collection on Gender in Physics.] Sexism occurs when men are believed to be superior to women, and is thought to be one of the reasons for women’s underrepresentation in physics and astronomy. The issue of sexism in physics and astronomy has not been thoroughly explored in the physics education literature and there is currently no clear language for discussing sexism in the field. This article seeks to begin a conversation on sexism in physics and astronomy and offer a starting point for language to discuss sexism in research groups and departments. Interviews with 21 women in graduate physics and astronomy programs are analyzed for their individual experiences of sexism. Although a subset of women did not report experiencing sexual discrimination, the majority experienced subtle insults and slights known as microaggressions. Other participants also experienced more traditional hostile sexism in the form of sexual harassment, gender role stereotypes, and overt discouragement. These results indicate the existence of sexism in the current culture of physics and astronomy, as well as the importance departments must put on eliminating it and educating students about sexism and microaggressions.

[This paper is part of the Focused Collection on Preparing and Supporting University Physics Educators.] The physics education research community has produced a wealth of knowledge about effective teaching and learning of college level physics. Based on this knowledge, many research-proven instructional strategies and teaching materials have been developed and are currently available to instructors. Unfortunately, these intensive research and development activities have failed to influence the teaching practices of many physics instructors. This paper describes interim results of a larger study to develop a model of designing materials for successful propagation. The larger study includes three phases, the first two of which are reported here. The goal of the first phase was to characterize typical propagation practices of education developers, using data from a survey of 1284 National Science Foundation (NSF) principal investigators and focus group data from eight disciplinary groups of NSF program directors. The goal of the second phase was to develop an understanding of successful practice by studying three instructional strategies that have been well propagated. The result of the first two phases is a tentative model of designing for successful propagation, which will be further validated in the third phase through purposeful sampling of additional well-propagated instructional strategies along with typical education development projects. We found that interaction with potential adopters was one of the key missing ingredients in typical education development activities. Education developers often develop a polished product before getting feedback, rely on mass-market communication channels for dissemination, and do not plan for supporting adopters during implementation. The tentative model resulting from this study identifies three key propagation activities: interactive development, interactive dissemination, and support of adopters. Interactive development uses significant feedback from potential adopters to develop a strong product suitable for use in many settings. Interactive dissemination uses personal interactions to reach and motivate potential users. Support of adopters is missing from typical propagation practice and is important to reduce the burden of implementation and increases the likelihood of successful adoption.

[This paper is part of the Focused Collection on Preparing and Supporting University Physics Educators.] The lack of knowledge about how to effectively spread and sustain the use of research-based instructional strategies is currently a significant barrier to the improvement of undergraduate physics education. In this paper we address this lack of knowledge by reporting on an interview study of 35 physics faculty, of varying institution types, who were self-reported users of, former users of, or knowledgeable nonusers of the research-based instructional strategy Peer Instruction. Interview questions included in this analysis focused on the faculty’s experiences, knowledge, and use of Peer Instruction, along with general questions about current and past teaching methods used by the interviewee. The primary findings include the following: (i) Faculty self-reported user status is an unreliable measure of their actual practice. (ii) Faculty generally modify specific instructional strategies and may modify out essential components. (iii) Faculty are often unaware of the essential features of an instructional strategy they claim to know about or use. (iv) Informal social interactions provide a significant communication channel in the dissemination process, in contrast to the formal avenues of workshops, papers, websites, etc., often promoted by change agents, and (v) experience with research-based strategies as a graduate student or through curriculum development work may be highly impactful. These findings indicate that educational transformation can be better facilitated by improving communication with faculty, supporting effective modification by faculty during implementation, and acknowledging and understanding the large impact of informal social interactions as a mode of dissemination.

While many innovative teaching strategies exist, integration into undergraduate science teaching has been frustratingly slow. This study aims to understand the low uptake of research-based instructional innovations by studying 21 successful implementations of the Student Centered Active Learning with Upside-down Pedagogies (SCALE-UP) instructional reform. SCALE-UP significantly restructures the classroom environment and pedagogy to promote highly active and interactive instruction. Although originally designed for university introductory physics courses, SCALE-UP has spread to many other disciplines at hundreds of departments around the world. This study reports findings from in-depth, open-ended interviews with 21 key contact people involved with successful secondary implementations of SCALE-UP throughout the United States. We defined successful implementations as those who restructured their pedagogy and classroom and sustained and/or spread the change. Interviews were coded to identify the most common enabling and challenging factors during reform implementation and compared to the theoretical framework of Kotter’s 8-step Change Model. The most common enabling influences that emerged are documenting and leveraging evidence of local success, administrative support, interaction with outside SCALE-UP user(s), and funding. Many challenges are linked to the lack of these enabling factors including difficulty finding funding, space, and administrative and/or faculty support for reform. Our focus on successful secondary implementations meant that most interviewees were able to overcome challenges. Presentation of results is illuminated with case studies, quotes, and examples that can help secondary implementers with SCALE-UP reform efforts specifically. We also discuss the implications for policy makers, researchers, and the higher education community concerned with initiating structural change.

[This paper is part of the Focused Collection on Preparing and Supporting University Physics Educators.] In order to promote sustained and impactful educational transformation, it is essential for change agents to understand more about faculty perceptions associated with either adopting or not adopting a research-based instructional strategy (RBIS). In this paper, we use interviews with 35 physics faculty to examine barriers and affordances to the use of the research-based instructional strategy of Peer Instruction. We found that the most common reasons faculty give for aligning their instruction with Peer Instruction is that it is not lecture and they have had positive experiences with Peer Instruction. The most common reasons faculty give for not using Peer Instruction are concerns about the time it will take, the loss of content coverage, and having had bad experiences with it. Additionally, we found the perceived barriers to be very different depending on whether the interviewee was a user of Peer Instruction or not, with nonusers being more concerned with time and users being more concerned with implementation difficulties. It is important for change agents to understand and address concerns faculty have about implementing research-based instructional strategies. Based on these results we offer four recommendations for those interested in promoting educational transformation toward research-based instructional strategies: (1) do not waste a lot of time criticizing lecture-based instruction and convincing faculty of the value of research-based strategies (they are already dissatisfied with lecture), (2) understand and address concerns faculty have about implementing active learning techniques, (3) focus on supporting and encouraging faculty experiences with RBIS, (4) address concerns faculty new to RBIS have about the time and energy needed to change.

To study the impact of the mitochondrial ubiquitin ligase MARCH5 on mitochondrial morphology and induction of apoptosis using an in vitro model of neuronal precursor cells exposed to glaucoma-relevant stress conditions.RGC5 cells transfected with expression constructs for MARCH5, MARCH5(H43W), Dpr1(K38A) or vector control were exposed to either elevated pressure of 30 mmHg, oxidative stress caused by mitochondrial electron transport chain (ETC) inhibition, or hypoxia-reoxygenation conditions. Mitochondrial morphology of RGC5 cells was analyzed following staining of the mitochondrial marker cytochrome c and photoactivatable GFP (PAGFP) diffusion assay. Induction of apoptotic cell death in these cells was determined by analyzing the release of cytochrome c from mitochondria into the cytosol and flow cytometry.Exposure of RGC5 cells to oxidative stress conditions as well as to elevated pressure resulted in the fragmentation of the mitochondrial network in control cells as well as in cells expressing MARCH5. In cells expressing inactive MARCH5(H43W) or inactive Drp(K38A), mitochondrial fragmentation was significantly blocked and mitochondrial morphology was comparable to that of control cells under normal conditions. Exposure of RGC5 cells to elevated pressure or oxidative stress conditions induced apoptotic cell death as assessed by cytochrome c release and DNA staining, while expression of dominant-negative MARCH5(H43W) or Drp1(K38A) did significantly delay cell death.Preventing mitochondrial fragmentation through interference with the mitochondrial fission machinery protects neuronal cells from programmed cell death following exposure to stressors physiologically relevant to the pathogenesis of glaucoma.

The human skin is a complex ecosystem that hosts a heterogeneous flora. Until recently, the diversity of the cutaneous microbiota was mainly investigated for bacteria through culture based assays subsequently confirmed by molecular techniques. There are now many evidences that viruses represent a significant part of the cutaneous flora as demonstrated by the asymptomatic carriage of beta and gamma-human papillomaviruses on the healthy skin. Furthermore, it has been recently suggested that some representatives of the Polyomavirus genus might share a similar feature. In the present study, the cutaneous virome of the surface of the normal-appearing skin from five healthy individuals and one patient with Merkel cell carcinoma was investigated through a high throughput metagenomic sequencing approach in an attempt to provide a thorough description of the cutaneous flora, with a particular focus on its viral component. The results emphasize the high diversity of the viral cutaneous flora with multiple polyomaviruses, papillomaviruses and circoviruses being detected on normal-appearing skin. Moreover, this approach resulted in the identification of new Papillomavirus and Circovirus genomes and confirmed a very low level of genetic diversity within human polyomavirus species. Although viruses are generally considered as pathogen agents, our findings support the existence of a complex viral flora present at the surface of healthy-appearing human skin in various individuals. The dynamics and anatomical variations of this skin virome and its variations according to pathological conditions remain to be further studied. The potential involvement of these viruses, alone or in combination, in skin proliferative disorders and oncogenesis is another crucial issue to be elucidated.

BACKGROUND:The Drosophila melanogaster gene CG11501 is up regulated after a septic injury and was proposed to act as a negative regulator of the JAK/STAT signaling pathway. Diedel, the CG11501 gene product, is a small protein of 115 residues with 10 cysteines. METHODOLOGY/PRINCIPAL FINDINGS:We have produced Diedel in Drosophila S2 cells as an extra cellular protein thanks to its own signal peptide and solved its crystal structure at 1.15 Å resolution by SIRAS using an iodo derivative. Diedel is composed of two sub domains SD1 and SD2. SD1 is made of an antiparallel β-sheet covered by an α-helix and displays a ferredoxin-like fold. SD2 reveals a new protein fold made of loops connected by four disulfide bridges. Further structural analysis identified conserved hydrophobic residues on the surface of Diedel that may constitute a potential binding site. The existence of two conformations, cis and trans, for the proline 52 may be of interest as prolyl peptidyl isomerisation has been shown to play a role in several physiological mechanisms. The genome of D. melanogaster contains two other genes coding for proteins homologous to Diedel, namely CG43228 and CG34329. Strikingly, apart from Drosophila and the pea aphid Acyrthosiphon pisum, Diedel-related sequences were exclusively identified in a few insect DNA viruses of the Baculoviridae and Ascoviridae families. CONCLUSION/SIGNIFICANCE:Diedel, a marker of the Drosophila antimicrobial/antiviral response, is a member of a small family of proteins present in drosophilids, aphids and DNA viruses infecting lepidopterans. Diedel is an extracellular protein composed of two sub-domains. Two special structural features (hydrophobic surface patch and cis/trans conformation for proline 52) may indicate a putative interaction site, and support an extra cellular signaling function for Diedel, which is in accordance with its proposed role as negative regulator of the JAK/STAT signaling pathway.

Extinction is an integral part of normal healthy fear responses, while it is compromised in several fear-related mental conditions in humans, such as post-traumatic stress disorder (PTSD). Although much research has recently been focused on fear extinction, its molecular and cellular underpinnings are still unclear. The development of animal models for extinction will greatly enhance our approaches to studying its neural circuits and the mechanisms involved. Here, we describe two gene-knockout mouse lines, one with impaired and another with enhanced extinction of learned fear. These mutant mice are based on fear memory-related genes, stathmin and gastrin-releasing peptide receptor (GRPR). Remarkably, both mutant lines showed changes in fear extinction to the cue but not to the context. We performed indirect imaging of neuronal activity on the second day of cued extinction, using immediate-early gene c-Fos. GRPR knockout mice extinguished slower (impaired extinction) than wildtype mice, which was accompanied by an increase in c-Fos activity in the basolateral amygdala and a decrease in the prefrontal cortex. By contrast, stathmin knockout mice extinguished faster (enhanced extinction) and showed a decrease in c-Fos activity in the basolateral amygdala and an increase in the prefrontal cortex. At the same time, c-Fos activity in the dentate gyrus was increased in both mutant lines. These experiments provide genetic evidence that the balance between neuronal activities of the amygdala and prefrontal cortex defines an impairment or facilitation of extinction to the cue while the hippocampus is involved in the context-specificity of extinction.

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Here we describe a Guadalupian brachiopod fauna from units III to V of the shallow water carbonate succession cropping out at Djebel Tebaga de Medenine, South Tunisia. The fauna comprises 29 taxa of the orders Productida, Orthotetida, Orthida, Rhynchonellida, Athyridida, Spiriferida and Terebratulida, whose distribution is consistent with the Wordian-Capitanian age suggested by the associated fusulinids and conodonts. Most of the brachiopod taxa are pedicle attached genera or cemented ones and thus were permanently attached, and only two were free living concavo-convex semi-infaunal genera; this indicates that they were mostly living in high energy environmental settings, where the settling strategies require firm attachment to the substrate at shallow depth under the influence of fair-weather waves. The brachiopods from South Tunisia are very similar to the fauna from the allochthonous limestones of Pietra di Salomone, Rupe del Passo di Burgio and Rocca di San Benedetto of Sosio Valley, Sicily, Italy, a fauna which is also dominated by cemented and pediculate taxa. Both regions were located at palaeoequatorial latitudes in the western termination of the Tethyan Gulf during Middle Permian time.

This study investigates how the beliefs and values of physics faculty influence their choice of physics problems for their students in an introductory physics course. The study identifies the goals these instructors have for their students, the problem features they believe facilitate those goals, and how those features correspond to problems they choose to use in their classes. This analysis comes from an artifact-based interview of 30 physics faculty teaching introductory calculus-based physics at a wide variety of institutions. The study concludes that instructors’ goals and the problem features they believe support those goals align with research-based curricular materials intended to develop competent problem solvers. However, many of these instructors do not use the beneficial problem features because they believe these features conflict with a more powerful set of values concerned with clarity of presentation and minimizing student stress, especially on exams.

The World Health Organization (WHO) proposes a successful closure rate for first repair of vesico-vaginal obstetric fistula to be at 85% in each facility, with the continence achievement among the closed cases at 90 %. We are reporting the vesico-vaginal obstetric fistula outcome at the provincial hospital of Maroua-Cameroon from 2005 to August 2007. Among the overall 32 patients with vesico-vaginal fistula operated, 25 patients were at their first operation. The complete closure of vesico-vaginal fistula (VVF) was 23/25 (92%) and among the 23 patients with complete closure 17(74%) had good continence. When we consider only the 25 patients who were at their first operation, the overall closure of VVF was 23/25 (92%) and among them 17/23 (74%) were continent. Large lesion, bladder neck lesions, vaginal adherence and rigid margin are associated with failure/incontinence. These factors must be taken into consideration when preparing patients for surgery or when assigning them to a surgeon within the surgical team

During the Fall of 2008 we designed and administered a web survey to collect information about pedagogical knowledge and practices of physics faculty. The survey was completed by a representative sample of 722 physics faculty across the United States (a 50.3% response rate). This paper presents results of one part of the survey where faculty were asked to rate their level of knowledge and use of 24 Research-Based Instructional Strategies (RBIS) that are applicable to an introductory quantitative physics course. Almost all faculty (87.1%) indicated familiarity with one or more RBIS and approximately half of faculty (48.1%) said that they currently use at least one RBIS. Results also indicate that faculty rarely use RBIS as recommended by the developer, but instead commonly make significant modifications.

Studies indicate that computed radiography (CR) can lead to increased radiation dose to patients. It is therefore important to relate the exposure indicators provided by CR manufacturers to the radiation dose delivered so as to assess the radiation dose delivered to patients directly from the exposure indicators. The aim of this study was to investigate the performance of an Agfa CR system in order to characterize the dose indicators provided by the system. The imaging plate response was characterized in terms of entrance exposure to the plate and the digital signal indicators generated by the system (SAL - Scanning Average Level and lgM - Logarithmic median) for different beam qualities. Several exposures were performed on a mammography unit and the digital signal, expressed as SAL and lgM for each image was correlated with the entrance exposure on a standard ACR phantom. From this, the relationship between the Agfa dose indices (SAL and lgM) and the average glandular dose (AGD) in mammography was established. An equation was derived to calculate the AGD delivered to the patient as a function of the exposure indicator, lgM, and the kV. The results indicated that the measured AGD at 28kV for a standard breast thickness during routine calibration with the ACR phantom was 1.58mGy (lgM = 1.99), which is within 1.5% of the value calculated using the derived equation for a standard Perspex thickness of 4.2cm using the AEC (1.56mGy). The standard error in using this equation was calculated to be 8.3%.

To identify and describe the basis upon which instructors make curricular and pedagogical decisions, we have developed an artifact-based interview and an analysis technique based on multilayered concept maps. The policy capturing technique used in the interview asks instructors to make judgments about concrete instructional artifacts similar to those they likely encounter in their teaching environment. The analysis procedure alternatively employs both an a priori systems view analysis and an emergent categorization to construct a multilayered concept map, which is a hierarchically arranged set of concept maps where child maps include more details than parent maps. Although our goal was to develop a model of physics faculty beliefs about the teaching and learning of problem solving in the context of an introductory calculus-based physics course, the techniques described here are applicable to a variety of situations in which instructors make decisions that influence teaching and learning.

Many proven research-based instructional strategies have been developed for introductory college-level physics. Significant efforts to disseminate these strategies have focused on convincing individual instructors to give up their traditional practices in favor of particular research-based practices. Yet evidence suggests that the findings of educational research are, at best, only marginally incorporated into typical introductory physics courses. In this paper we present partial results of an interview study designed to generate new ideas about why proven strategies are slow to integrate in mainstream instruction. Specifically we describe the results of open-ended interviews with five physics instructors who represent likely users of educational research. We found that these instructors have conceptions about teaching and learning that are more compatible with educational research than with their self-described instructional practices. Instructors often blamed this discrepancy on situational factors that favor traditional instruction. A theoretical model is introduced to explain these findings.

We present and describe the development of a framework for assessing or categorizing instructional practices and related conceptions in the context of introductory physics instruction. The purpose of the framework is for articulating practices and conceptions of individual instructors or of curricula. It was developed based on the research literature and interviews with physics faculty and contains ideas with widespread acceptance as well as some outside mainstream rhetoric. We provide an example of how this framework can be applied by using it to categorize aspects of three well-known research-based curricula. The results of this categorization highlight areas that are common in research-based curricular designs and other areas that are less embraced but worthy of consideration.

Women currently outnumber men in obtaining undergraduate degrees but are underrepresented within STEM fields. However, women’s representation varies by STEM field, and even further by STEM subfield. One field that has held a persistent low representation of women is physics. This paper seeks to uncover the truth behind an anecdotal claim that the subfield of physics education research (PER) has a higher representation of women than physics as a whole. Graduate students in PER completed an online survey to assess their demographics, trajectory in PER, climate experiences, and goals for their research. The response rate for the survey was 68%, yielding 125 total respondents. This paper will focus on the 91 respondents enrolled in U.S. graduate programs. It was found that women make up 51% of the U.S. PER graduate students in this sample, as compared to only 19% of physics graduate students overall. Survey findings also revealed that both women and men in PER graduate programs experience similarly positive working relationships with faculty and fellow students. Last, both men and women reported building a stronger scientific workforce and becoming better teachers as goals for their PER research.

Recension

Physics education research (PER) is a rapidly growing area of PhD specialization. In this article we examine the trajectories that led respondents into a PER graduate program as well as their expected future trajectories. Data were collected in the form of an online survey sent to graduate students in PER. Our findings show a lack of visibility of PER as a field of study, a dominance of work at the undergraduate level, and a mismatch of future desires and expectations. We suggest that greater exposure is needed so PER is known as a field of inquiry for graduates, that more emphasis should be placed on research beyond the undergraduate level, and that there needs to be stronger communication to graduate students about potential careers.