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Vibrio alginolyticus infection is common in aquatic species. The species has been isolated from infected green mussels (Perna viridis) cultivated at Marudu Bay, Sabah. The infection of V. alginolyticus was prevalent since 2009 and caused more than 60% mortality of the mollusc. This study was conducted on V. alginolyticus isolated from clinically infected P. viridis at cultivation area in Marudu Bay from 2013. Twenty isolates were randomly selected and subjected to RAPD-PCR analysis using 20 sets of RAPD primers. Results showed that the most suitable primers were OPA 3, OPA 5, OPA 9, OPA 10 and OPA 12 where the significant bands ranging from 100-2500 bp. Genetic variability among the isolates as revealed by these primers indicated that RAPD-PCR method has good discriminative ability and can be used as a rapid typing method of differentiating V. alginolyticus strains isolated from infected green mussels for epidemiological investigation.

Vibrio alginolyticus infection is common in aquatic species. The species has been isolated from infected green mussels (Perna viridis) cultivated at Marudu Bay, Sabah. The infection of V. alginolyticus was prevalent since 2009 and caused more than 60% mortality of the mollusc. This study was conducted on V. alginolyticus isolated from clinically infected P. viridis at cultivation area in Marudu Bay from 2013. Twenty isolates were randomly selected and subjected to RAPD-PCR analysis using 20 sets of RAPD primers. Results showed that the most suitable primers were OPA 3, OPA 5, OPA 9, OPA 10 and OPA 12 where the significant bands ranging from 100-2500 bp. Genetic variability among the isolates as revealed by these primers indicated that RAPD-PCR method has good discriminative ability and can be used as a rapid typing method of differentiating V. alginolyticus strains isolated from infected green mussels for epidemiological investigation.

The volatile chemicals from species of wild Cinnamomum spp. (C. racemosum, C. cuspidatum, C. politum, C. javanicum), Etlingera spp. (E. pyramidosphaera, E. megalocheilos, E. coccinea, E. elatior) and Schizostachyum spp. (S. blumei, S. brachycladum, S. lima, S. pilosum) found in Sabah were investigated. The oils were obtained from the bark, rhizome and culm of respective specimens by hydrodistillation and the profile of volatile chemicals was obtained using Gas Chromatography- Mass Spectrometry (GCMS). Dominance of eucalyptol, terpinen-4-ol and eugenol were consistent among the species from genus Cinnamomum. Aromadendrane oxide, lauryl aldehyde, elemicin, borneol and 1-dodecanol were predominant among the species from genus Etlingera. α-elemol, coumaran, guiacol-4-vinyl, palmitic acid and phytol acetate predominate the species from genus Schizostachyum. Strong inhibition against Staphylococcus aureus (MIC: 5.62 ± 0.5 μg mL-1) were exhibited by essential oils of C. cuspidatum and E. coccinea, oil of S. blumei inhibited Listeria monocytogenes (MIC: 4.60 ± 0.5 μg mL-1), oil of C. javanicum inhibited Salmonella typhimurium (MIC: 5.50 ± 0.5 μg mL-1). Meanwhile the oil of C. politum suppressed Salmonella enteritidis (MIC: 5.20 ± 0.5 μg mL-1) was measured using microdilution method. These findings reveal the potential of selected plants used by indigenous communities of Borneo as antimicrobials in food, cosmetics and pharmaceutical indu

The volatile chemicals from species of wild Cinnamomum spp. (C. racemosum, C. cuspidatum, C. politum, C. javanicum), Etlingera spp. (E. pyramidosphaera, E. megalocheilos, E. coccinea, E. elatior) and Schizostachyum spp. (S. blumei, S. brachycladum, S. lima, S. pilosum) found in Sabah were investigated. The oils were obtained from the bark, rhizome and culm of respective specimens by hydrodistillation and the profile of volatile chemicals was obtained using Gas Chromatography- Mass Spectrometry (GCMS). Dominance of eucalyptol, terpinen-4-ol and eugenol were consistent among the species from genus Cinnamomum. Aromadendrane oxide, lauryl aldehyde, elemicin, borneol and 1-dodecanol were predominant among the species from genus Etlingera. α-elemol, coumaran, guiacol-4-vinyl, palmitic acid and phytol acetate predominate the species from genus Schizostachyum. Strong inhibition against Staphylococcus aureus (MIC: 5.62 ± 0.5 μg mL-1) were exhibited by essential oils of C. cuspidatum and E. coccinea, oil of S. blumei inhibited Listeria monocytogenes (MIC: 4.60 ± 0.5 μg mL-1), oil of C. javanicum inhibited Salmonella typhimurium (MIC: 5.50 ± 0.5 μg mL-1). Meanwhile the oil of C. politum suppressed Salmonella enteritidis (MIC: 5.20 ± 0.5 μg mL-1) was measured using microdilution method. These findings reveal the potential of selected plants used by indigenous communities of Borneo as antimicrobials in food, cosmetics and pharmaceutical indu

Vibrio alginolyticus infection is common in aquatic species. The species has been isolated from infected green mussels (Perna viridis) cultivated at Marudu Bay, Sabah. The infection of V. alginolyticus was prevalent since 2009 and caused more than 60% mortality of the mollusc. This study was conducted on V. alginolyticus isolated from clinically infected P. viridis at cultivation area in Marudu Bay from 2013. Twenty isolates were randomly selected and subjected to RAPD-PCR analysis using 20 sets of RAPD primers. Results showed that the most suitable primers were OPA 3, OPA 5, OPA 9, OPA 10 and OPA 12 where the significant bands ranging from 100-2500 bp. Genetic variability among the isolates as revealed by these primers indicated that RAPD-PCR method has good discriminative ability and can be used as a rapid typing method of differentiating V. alginolyticus strains isolated from infected green mussels for epidemiological investigation.

Vibrio alginolyticus infection is common in aquatic species. The species has been isolated from infected green mussels (Perna viridis) cultivated at Marudu Bay, Sabah. The infection of V. alginolyticus was prevalent since 2009 and caused more than 60% mortality of the mollusc. This study was conducted on V. alginolyticus isolated from clinically infected P. viridis at cultivation area in Marudu Bay from 2013. Twenty isolates were randomly selected and subjected to RAPD-PCR analysis using 20 sets of RAPD primers. Results showed that the most suitable primers were OPA 3, OPA 5, OPA 9, OPA 10 and OPA 12 where the significant bands ranging from 100-2500 bp. Genetic variability among the isolates as revealed by these primers indicated that RAPD-PCR method has good discriminative ability and can be used as a rapid typing method of differentiating V. alginolyticus strains isolated from infected green mussels for epidemiological investigation.