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Perfluorooctane sulfonamide (FOSA) is known as a key intermediate of perfluorooctane sulfonic acid (PFOS) precursors, which can be frequently detected in the environment and biota. FOSA could be bioaccumulated in earthworms from soil, but the contributions of enzymes and gut microbes involved in the biotransformation of FOSA in earthworms have not been identified. Therefore, the effects of enzyme inhibitors and intestinal microflora on biotransformation of FOSA in earthworms were investigated in the present study. FOSA was biotransformed to form PFOS by earthworms obtained from in vivo and in vitro tests. The addition of FOSA had significantly positive effects on cytolchrome P450 (CYP450) and glutathione-s-transferase (GST) activities, suggesting CYP450 and GST are likely involved in the enzymatic transformation. In addition, both 1-Aminobenzotriazole (ABT) and ezatiostat hydrochloride (TLK199), which were selected to inhibit the CYP and GST enzymes, respectively, demonstrated inhibition effects on biotransformation of FOSA in earthworms with a dose-dependent relationship. However, the concentrations of FOSA weren't changed by the bacteria isolated from worm gut, suggesting that gut bacteria did not contribute to FOSA biotransformation in earthworms. The results of this study confirm that the transformation of FOSA in earthworms is mediated mainly by enzymes rather than by gut microbes., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

New Jersey (NJ) residents in some areas may be exposed to perfluoroalkyl and polyfluoroalkyl substances (PFAS) due to PFAS contamination of public drinking water. This contamination stems from industrial discharges and the use of aqueous film-foaming foams at military bases and commercial airports for drills and locations where fires occurred. Exposure to PFAS has raised significant public health concerns due to its persistence both in the environment and human body. The potential toxicity of these chemicals may pose risks to human health. Statewide biomonitoring data is needed to establish a baseline of exposure and to identify the subpopulations at risk. The NJ Department of Health (NJDOH) conducted a statewide NJ Biomonitoring (NJBM) study for PFAS utilizing a cost-effective sampling approach, i.e. acquiring remnant sera from both clinical laboratories and blood banks across NJ. This convenience sampling approach was adopted as an alternative to a costly and labor-intensive probability-based population sampling. One thousand and thirty human sera were collected from NJ adults between 20 and 74 years of age from 2016 to 2018, with additional information of county, sex, and age. The serum collection was demographically and geographically dispersed across four seasons. Twelve PFAS analytes were measured for the specimens collected. The data were post-stratified by county, sex, and age groups (20-39, 40-59, and 60-74 years old). Stratified individual sample weights were developed and used to estimate population means, compare least-squared mean differences, and examine contributing variables. Geometric means (GMs) and percentiles with 95% confidence intervals (CIs) of the target analytes are presented, providing preliminary baselines of the statewide PFAS exposure for NJ adults. PFOA, PFNA, PFHxS, and PFOS were selected for in-depth analyses because their GMs were greater than 0.5 ng/mL and they were detected over 99% in study population. Subjects from this study had higher serum levels of PFOA, PFNA, and PFHxS compared to the general U.S. population reported by the latest National Health and Nutrition Examination Survey (NHANES in 2015-2016). However, the distributions of PFOA, PFNA, PFHxS, and PFOS across sex and age groups were consistent with the patterns found in NHANES, i.e. all differed by sex and age group. Further sex and age stratification showed significantly lower concentrations of the 4 analytes in younger females (20-59 years old) than in older females (60-74 years old) and males (20-74 years old). Future research is needed to identify PFAS exposure sources and to develop effective intervention strategies. Continuing PFAS biomonitoring using population sampling is recommended for tracking trends and better identifying subpopulations at risk., (Copyright © 2019 Elsevier GmbH. All rights reserved.)

Per- and polyfluoroalkyl substances (PFASs) have been used for decades within industrial processes and consumer products, resulting in frequent detection within the environment. Using zebrafish embryos, we screened 38 PFASs for developmental toxicity and revealed that perfluorooctanesulfonamide (PFOSA) was the most potent developmental toxicant, resulting in elevated mortality and developmental abnormalities following exposure from 6 to 24 h post fertilization (hpf) and 6 to 72 hpf. PFOSA resulted in a concentration-dependent increase in mortality and abnormalities, with surviving embryos exhibiting a >12-h delay in development at 24 hpf. Exposures initiated at 0.75 hpf also resulted in a concentration-dependent delay in epiboly, although these effects were not driven by a specific sensitive window of development. We relied on mRNA-sequencing to identify the potential association of PFOSA-induced developmental delays with impacts on the embryonic transcriptome. Relative to stage-matched vehicle controls, these data revealed that pathways related to hepatotoxicity and lipid transport were disrupted in embryos exposed to PFOSA from 0.75 to 14 hpf and 0.75 to 24 hpf. Therefore, we measured liver area as well as neutral lipids in 128-hpf embryos exposed to vehicle (0.1% DMSO) or PFOSA from 0.75 to 24 hpf and clean water from 24 to 128 hpf, and showed that PFOSA exposure from 0.75 to 24 hpf resulted in a decrease in liver area and increase in yolk sac neutral lipids at 128 hpf. Overall, our findings show that early exposure to PFOSA adversely impacts embryogenesis, an effect that may lead to altered lipid transport and liver development., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Background: Epidemiological evidence remains equivocal on the associations between environmentally relevant levels of per-/polyfluoroalkyl substances (PFASs) and human semen quality., Objectives: We aimed to test whether the potential effects on semen quality could be better observed when seminal PFAS levels were used as an exposure marker compared with serum PFAS levels., Methods: Matched semen and serum samples from 664 adult men were collected from a cross-sectional population in China from 2015 to 2016. Multiple semen parameters were assessed, along with measurement of 16 target PFASs in semen and serum. Partitioning between semen and serum was evaluated by the ratio of matrix-specific PFAS concentrations. Regression model results were expressed as the difference in each semen parameter associated with the per unit increase in the ln-transformed PFAS level after adjusting for confounders., Results: Perfluorooctanoate (PFOA), perfluorooctane sulfonate (PFOS), and emerging chlorinated polyfluorinated ether sulfonate (6:2 Cl-PFESA) were detected at their highest concentrations in both semen and serum, with median concentrations of 0.23, 0.10, and 0.06   ng / mL in semen, respectively, and a semen-to-serum ratio of 1.3:3.1. The between-matrix correlations of these PFAS concentrations were high ( R = 0.70 - 0.83 ). Seminal PFOA, PFOS, and 6:2 Cl-PFESA levels were significantly associated with a lower percentage of progressive sperm and higher percentage of DNA fragmentation (false discovery rate-adjusted p -values   of < 0.05 ). Associations between serum PFAS levels and semen parameters were generally statistically weaker, except for DNA stainability, which was more strongly associated with serum-based PFASs than with semen-based PFASs., Conclusions: Our results suggest the potential for deleterious effects following exposure to 6:2 Cl-PFESA and other PFASs. Compared with serum PFAS levels, the much clearer association of seminal PFAS levels with semen parameters suggests its advantage in hazard assessment on semen quality, although the potential for confounding might be higher. Exposure measurements in target tissue may be critical in clarifying effects related to PFAS exposure. https://doi.org/10.1289/EHP4431.

Hypoxic pulmonary vasoconstriction (HPV) is a well-characterized vascular response to low oxygen pressures and is involved in life-threatening conditions such as high-altitude pulmonary edema (HAPE) and pulmonary arterial hypertension (PAH). While the efficacy of oral therapies can be affected by drug metabolism, or dose-limiting systemic toxicity, inhaled treatment via pressured metered dose inhalers (pMDI) may be an effective, nontoxic, practical alternative. We hypothesized that a stable water-in-perfluorooctyl bromide (PFOB) emulsion that provides solubility in common pMDI propellants, engineered for intrapulmonary delivery of pulmonary vasodilators, reverses HPV during acute hypoxia (HX). Male Sprague Dawley rats received two 10-min bouts of HX (13% O 2 ) with 20 min of room air and drug application between exposures. Treatment groups: intrapulmonary delivery (PUL) of (1) saline; (2) ambrisentan in saline (0.1 mg/kg); (3) empty emulsion; (4) emulsion encapsulating ambrisentan or sodium nitrite (NaNO 2 ) (0.1 and 0.5 mg/kg each); and intravenous (5) ambrisentan (0.1 mg/kg) or (6) NaNO 2 (0.5 mg/kg). Neither PUL of saline or empty emulsion, nor infusions of drugs prevented pulmonary artery pressure (PAP) elevation (32.6 ± 3.2, 31.5 ± 1.2, 29.3 ± 1.8, and 30.2 ± 2.5 mmHg, respectively). In contrast, PUL of aqueous ambrisentan and both drug emulsions reduced PAP by 20-30% during HX, compared to controls. IL6 expression in bronchoalveolar lavage fluid and whole lung 24 h post-PUL did not differ among cohorts. We demonstrate proof-of-concept for delivering pulmonary vasodilators via aerosolized water-in-PFOB emulsion. This concept opens a potentially feasible and effective route of treating pulmonary vascular pathologies via pMDI.

Objectives: The aim of the present study was to examine the association between exposure to polyfluoroalkyl substances (PFASs) and mortality (1970-2018) in a cohort of 462 male employees who had worked at least six months before 2009 for a factory (14,658 person-years; 107 deaths, average follow-up time 31.7 years), which had been producing perfluorooctanoic acid (PFOA), perfluorooctanesulfonyl fluoride (PFOS) and other chemicals since 1968., Methods: Employees were classified as follows: 1) by probability of exposure to PFASs; 2) by tertiles of PFOA serum concentrations. In a fraction (n = 120) of workers measurements of internal PFOA serum concentration were used to predict a cumulative serum PFOA concentration of each cohort member. Mortality rates were compared to that of the regional population using the standardized mortality ratio (SMR), and to that of the workers of a nearby metalworking factory in terms of risk ratio (RR), across categories of probability of PFASs exposure and tertiles of cumulative serum PFOA concentrations., Results: Internal PFOA serum concentration among 120 workers in the 2000-2013 period was very high (Geometric Mean: 4048 ng/mL; range 19-91,900 ng/mL). The mortality of the chemical cohort was increased for liver cancer (SMR: 2.32; CI: 1.11-4.87), malignant neoplasm of lymphatic and haematopoietic tissue (SMR: 2.26; CI: 1.08-4.73). In the comparison with the cohort of workers from the metalworking factory, the RRs for mortality of the cohort were increased for overall mortality (RR: 1.42; CI: 1.12-1.79), diabetes (RR: 5.95; CI: 1.08-32.8), liver cancer (RR: 6.69; CI: 1.71-26.2) and liver cirrhosis (RR: 3.87; CI: 1.18-12.7). Mortality for these causes increased in association with probability of PFASs exposure and with tertiles of cumulative PFOA serum concentrations., Conclusion: The present is a small observational study with limited control over confounding factors. The cohort showed increased mortality for all causes and subjects in the highest cumulative internal dose of PFOA had a statistically significant increase for mortality of liver cancer, liver cirrhosis, diabetes, malignant neoplasms of lymphatic and haematopoietic tissue in both comparisons. Toxicological studies on PFOA and PFOS provide support for causality for the observed association with the risk for liver cirrhosis and liver cancer., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Perfluorooctane sulfonate (PFOS) has been widely utilized in numerous industries. Due to long environmental and biological half-lives, PFOS is a major public health concern. Although the literature suggests that PFOS may induce neurotoxicity, neurotoxic mechanisms, and neuropathology are poorly understood. Thus, the primary goal of this study was to determine if PFOS is selectively neurotoxic and potentially relevant to specific neurological diseases. Nematodes (Caenorhabditis elegans) were exposed to PFOS or related per- and polyfluoroalkyl substances (PFAS) for 72 h and tested for evidence of neuropathology through examination of cholinergic, dopaminergic, gamma-amino butyric acid (GABA)ergic, and serotoninergic neuronal morphologies. Dopaminergic and cholinergic functional analyses were assessed through 1-nonanol and Aldicarb assay. Mechanistic studies assessed total reactive oxygen species, superoxide ions, and mitochondrial content. Finally, therapeutic approaches were utilized to further examine pathogenic mechanisms. Dopaminergic neuropathology occurred at lower exposure levels (25 ppm, approximately 50 µM) than required to produce neuropathology in GABAergic, serotonergic, and cholinergic neurons (100 ppm, approximately 200 µM). Further, PFOS exposure led to dopamine-dependent functional deficits, without altering acetylcholine-dependent paralysis. Mitochondrial content was affected by PFOS at far lower exposure level than required to induce pathology (≥1 ppm, approximately 2 µM). Perfluorooctane sulfonate exposure also enhanced oxidative stress. Further, mutation in mitochondrial superoxide dismutase rendered animals more vulnerable. Neuroprotective approaches such as antioxidants, PFAS-protein dissociation, and targeted (mitochondrial) radical and electron scavenging were neuroprotective, suggesting specific mechanisms of action. In general, other tested PFAS were less neurotoxic. The primary impact is to prompt research into potential adverse outcomes related to PFAS-induced dopaminergic neurotoxicity in humans., (© The Author(s) 2019. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Per- and poly-fluorinated substances (PFAS) are widely found in freshwater ecosystems because of their resistance to degradation. Among them, several long-chain perfluoroalkyl acids bioaccumulate in aquatic vertebrates, but our understanding of the mechanisms of absorption, distribution and elimination is still limited in fish. For this purpose, we developed a 10-compartment physiologically-based toxicokinetic (PBTK) model to elucidate perfluorooctane sulfonate (PFOS) kinetics in adult rainbow trout. This PBTK model included various physiological characteristics: blood perfusion to each organ, plasmatic fraction, PFOS free fraction, and growth of individuals. The parameters were optimized using Bayesian inferences. First, only PFOS absorption by diet was considered in the model as well as its elimination by urine, bile and feces. Then two mechanistic hypotheses, assumed to govern PFOS toxicokinetics in fish, namely the enterohepatic cycle and the absorption and elimination though gills, were tested. Improvement of the model's fit to the data was studied in each organ by comparing predictions with observed data using relative error. The experimental data set was obtained from an exposure experiment, where adult rainbow trout were fed with a PFOS-spiked diet for 42 days, followed by a 35-day depuration period. In all cases, PFOS concentrations were accurately predicted in organs and feces by the model. The results of this PBTK model demonstrated that feces represented the major elimination route for PFOS while urine was a minor route. Also, PFOS branchial uptake can be substantial despite low concentrations of the compound in water, and elimination through gills should not be neglected. Finally, the enterohepatic cycle is likely to play a minor role in PFOS toxicokinetics. Overall, this PBTK model accurately described PFOS distribution in rainbow trout and provides information on the relative contribution of absorption and elimination pathways., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The present work examined the trophic transfer of perfluoroalkyl and polyfluoroalkyl substances (PFASs) in a typical urban river (Orge River, near Paris, France), and aimed to investigate the potential contribution of precursors to the biomagnification of perfluoroalkyl acids (PFAAs). Sixteen PFAAs, twelve of their precursors (pre-PFAAs targeted ) and two fluorinated alternatives to long-chain PFASs were analyzed in water, sediments and biota (including biofilm, invertebrates and fish). Twenty two compounds were detected in biological samples (2.0-147 ng g -1 wet weight), perfluorooctane sulfonate (PFOS) and C 12 -C 14 perfluoroalkyl carboxylates (PFCAs) being predominant while ∑pre-PFAAs targeted contributed to 1-18% of ∑PFASs. Trophic magnification factors (TMFs) were >1 (i.e. denoting biomagnification) for C 9 -C 14 PFCAs, C 7 -C 10 perfluoroalkyl sulfonates (PFSAs) and several pre-PFAAs (e.g. 8 : 2 and 10 : 2 fluorotelomer sulfonates). The significant decrease in ∑pre-PFCAs/∑PFCAs concentration ratio with trophic level suggested a likely contribution of selected precursors to the biomagnification of PFCAs through biotransformation, while this was less obvious for PFOS. The total oxidizable precursor assay, applied for the first time to sediment and biota, revealed the presence of substantial proportions of extractable unknown pre-PFAAs in all samples (i.e. 15-80% of ∑PFASs upon oxidation). This proportion significantly decreased from sediments to invertebrates and fish, thereby pointing to the biotransformation of unattributed pre-PFAAs in the trophic web, which likely contributes to the biomagnification of some PFAAs (i.e. C 9 -C 12 PFCAs and C 7 -C 10 PFSAs).

Perfluoroalkyl acids (PFAAs) are persistent in the environment, highly bio-accumulative in the body, and likely hepatotoxic in humans. There is evidence of sex-specific physiological responses to PFAA exposure. However, epidemiological studies seldom stratify the analyses by sex. Given the high prevalence of liver disease in general population adolescents, this study was designed to determine whether or not there is association between exposure to PFAAs and biomarkers of liver function in adolescent participants of the 2013-2016 National Health and Nutrition Examination Survey, and whether or not such association is sex-specific. Multivariate linear regressions were performed to examine the association between single PFAAs [perfluorooctane sulfonic acid (PFOS); linear form of perfluorooctanoic acid (PFOA); perfluorohexane sulfonic acid (PFHxS); perfluorononanoic acid (PFNA)], and biomarkers of liver function - gamma glutamyltransferase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin. Multivariate logistic regressions were performed to estimate adjusted odd ratios (aOR) of elevated ALT, AST and GGT. The study results show that, in females, there was a positive association of the highest PFOA quartile with increased ALT, AST and GGT, and the highest PFNA quartile with increased ALT and AST. Conversely, in male adolescents there was an association of the highest linear PFOA quartile with decreased ALT, and the highest PFNA quartile with ALT and AST. Females had higher odds of clinically-defined elevated ALT with increased PFOA (aOR = 1.79; 95% CI: 1.05, 3.04) or PFNA (aOR = 2.28; 95% CI: 1.08, 2.28), whereas males had decreased odds of clinically-defined elevated ALT with increased n-PFOA (aOR = 0.43; 95% CI: 0.20, 0.93) or PFNA (aOR = 0.5; 95% CI: 0.28, 0.89). In conclusion, there were sex differences in the association between serum PFAA levels and biomarkers of liver function. These results may provide support for analyzing sex-based adverse effects of PFAAs., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Major point sources of per- and polyfluoroalkyl substances (PFASs) cause ubiquitous spread of PFASs in the environment. In this study, surface water and aquatic invertebrates at three Swedish sites impacted by PFAS point sources were characterized, using homologue, isomer and extractable organofluorine (EOF) profiling as well as estimation of bioaccumulation factors (BAFs) and mass discharge. Two sites were impacted by fire training (sites A and R) and the third by industrial runoff (site K). Mean Σ 25 PFASs concentration in water was 1920 ng L -1 at site R (n = 3), which was more than 20- and 10-fold higher than those from sites A and K, respectively. PFOS was the most predominant PFAS in all waters samples, constituting 29-79% of Σ 25 PFAS concentrations. Several branched isomers were detected and they substantially contributed to concentrations in surface water (e.g. 49-78% of ΣPFOS) and aquatic invertebrates (e.g. 15-28% of ΣPFOS). BAFs in the aquatic invertebrates indicated higher bioaccumulation for long chain PFASs and lower bioaccumulation for branched PFOS isomers compared to linear PFOS. EOF mass balance showed that Σ 25 target PFASs in water could explain up to 55% of EOF at site R. However, larger proportions of EOF (>92%) remained unknown in water from sites A and K. Mass discharges were for the first time estimated for EOF and revealed that high amounts of EOF (e.g. 8.2 g F day -1 at site A) could be transported by water to recipient water bodies relative to Σ 25 PFASs (e.g. 0.15 g day -1 at site A). Overall, we showed that composition profiling, BAFs and EOF mass balance can improve the characterization of PFASs around point sources.

Although many studies have evaluated the fate of per- and polyfluoroalkyl acids (PFAAs) in aquatic environments, few have observed their fate in terrestrial environments. It has been proposed that ingestion could be a major PFAA exposure route for humans. We determined PFAA uptake in radish, carrot, and alfalfa under a maximum bioavailability scenario. Bioconcentration factors (BCFs) were determined in the edible tissue of radish (perfluorobutanesulfonate [PFBS] = 72; perfluorohexanesulfonate [PFHxS] = 13; perfluoroheptanoate [PFHpA] = 65; perfluorooctanoate [PFOA] = 18; perfluorooctanesulfonate [PFOS] = 2.9; and perfluorononanoate [PFNA] = 9.6), carrot (PFBS = 5.9; PFHxS = 1.1; PFHpA = 29; PFOA = 3.1; PFOS = 1; and PFNA = 1.4), and alfalfa (PFBS = 107; PFHxS = 12; PFHpA = 91; PFOA = 10; PFOS = 1.4; and PFNA = 1.7). Some of these PFAA BCFs are as much as 2 orders of magnitude higher than those measured previously in plants grown in biosolid-amended soils. Environ Toxicol Chem 2019;38:2497-2502. © 2019 SETAC., (© 2019 SETAC.)

While N-ethyl perfluorooctane sulfonamidoethanol (EtFOSE) is a precursor of perfluorooctane sulfonate (PFOS), its bioaccumulation, transformation and toxicological effects in earthworms (Eisenia fetida) exposed to quartz sands are poorly understood. The present study showed that except for parent EtFOSE, N-ethylperfluorooctane sulfonamide acetate (EtFOSAA), N-ethyl perfluorooctane sulfonamide (EtFOSA), perfluorooctane sulfonamide acetate (FOSAA), perfluorooctane sulfonamide (FOSA) and PFOS were detected in earthworms, with EtFOSAA as the primary biotransformation product. The biota-to-sand accumulation factor (BSAF) and uptake rate coefficient (k u ) of EtFOSE were 5.7 and 0.542/d, respectively. The elimination rate constants (k e ) decreased in the order EtFOSA (0.167/d) ∼ FOSAA (0.147/d) > FOSA (0.119/d) ∼ EtFOSAA (0.117/d) > EtFOSE (0.095/d) > PFOS (0.069/d). No significant effects were observed in malondialdehyde (MDA) contents and acetylcholinesterase (AChE) activities between EtFOSE treatments and controls. EtFOSE could cause significant accumulation of reactive oxygen species (ROS) in earthworms. Peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) were significantly activated by 41.4-74.3%, 37.2-44.4% and 32.4-52.3% from day 4-10, respectively, while 8-Hydroxy-2-deoxyguanosine (8-OHdG) levels were elevated by 47.7-70.3% from day 8-10, demonstrating that EtFOSE induced oxidative stress and oxidative DNA damage in earthworms. Significant increase of glutathione-S-transferase (GST) with 41.6-62.8% activation (8-10 d) gave indirect evidence on the conjugation of EtFOSE or its corresponding metabolites during phase II of detoxication. This study provides important information on the fate and potential risks of EtFOSE to terrestrial invertebrates., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Titanium dioxide nanoparticles (TiO 2 -NPs) are widely distributed in the environment. It has been demonstrated that TiO 2 -NPs could modify the environmental fate and bioavailability of organic pollutants, which affects ecological risks of TiO 2 -NPs and organic pollutants. In this study, the uptake, translocation and accumulation of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonate (PFOS) in pumpkin plants was investigated in the presence of TiO 2 -NPs. We reported for the first time the negligible effects of TiO 2 -NPs at environmentally relevant concentrations (0.05-5 mg/L) on the uptake and accumulation of PFOA and PFOS in hydroponically grown pumpkin seedlings regardless of root, stem and leaf. This phenomenon was independent of the initial concentrations of PFOA/PFOS and TiO 2 -NPs in the exposure solution. Also, seedling mass and contents of chlorophyll and anthocyanin were not affected by the co-exposure. Adsorption tests demonstrated the negligible adsorption of PFOA/PFOS on TiO 2 -NPs in the exposure solution. Moreover, uptake of PFOA/PFOS was insensitive to aquaporin inhibitor AgNO 3 but significantly inhibited by niflumic acid (anion channel blocker) and 2,4-dinitrophenol (metabolic inhibitor) whereas Ti concentration in root was not affected by niflumic acid and 2,4-dinitrophenol but significantly decreased by AgNO 3 , indicating that transport of PFOA/PFOS and TiO 2 -NPs were via different routes into the pumpkin seedling. It was proposed that different pathways by which TiO 2 -NPs and PFOA/PFOS transported into the pumpkin seedling and negligible adsorption of PFOA/PFOS on TiO 2 -NPs contributed to the negligible effects of TiO 2 -NPs on the uptake, translocation and accumulation of PFOA/PFOS in pumpkin seedlings. In total, this work would improve our understanding of the ecological risks of TiO 2 -NPs in the environment., (Copyright © 2019. Published by Elsevier B.V.)

The wide application of 6:2 fluorotelomer sulfonate (6:2 FTS) and its precursors results in their ubiquitous occurrence in the environment. This study investigated the biotransformation of 6:2 FTS using fresh slurries collected from constructed wetland under aerobic and anoxic conditions. Biotransformation rates of 6:2 FTS was extremely slow under both aerobic and anoxic conditions. The analysis of transformation products showed that less than 0.7 mol% of initial concentration of 6:2 FTS was biotransformed under anoxic conditions. Effects of 6:2 FTS on the shifts in microbial community in wetland slurry during aerobic and anoxic transformation were evaluated using 16S rRNA gene amplicon sequencing. Under aerobic conditions, 6:2 FTS altered the microbial community structure, in which Methylocaldum (Gamma-proteobacteria) became the predominant genus after being exposed to 6:2 FTS. This means that Methylocaldum appears to have higher tolerance to 6:2 FTS and seems to be potential 6:2 FTS-degrading bacteria. Under anoxic transformation, only the genus Methanomethylovorans (Euryarchaeota) was increased remarkably in wetland slurry exposed to 6:2 FTS., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Poly- and perfluoroalkyl substances (PFAS) are ubiquitous contaminants that can bioaccumulate in aquatic taxa. Amphibians are particularly vulnerable to contaminants and sensitive to endocrine disruptors during their aquatic larval stage. However, few studies have explored PFAS uptake rates in amphibians, which is critical for designing ecotoxicology studies and assessing the potential for bioaccumulation. Uptake rates of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) were measured for larval northern leopard frogs (Rana pipiens), American toads (Anaxyrus americanus), and eastern tiger salamanders (Ambystoma tigrinum) during a 240-h exposure to 10 and 1000 μg/L concentrations. We measured body burden and calculated bioconcentration factor (BCF) every 48 h during the experiments. For all species and exposures, body burdens often reached steady state within 48-96 h of exposure. Steady-state body burdens for PFOA and PFOS ranged from 3819 to 16,481 ng/g dry weight (BCF = 0.46-2.5) and 6955-489,958 ng/g dry weight (47-259 BCFs), respectively. Therefore, PFAS steady state occurs rapidly in the larval amphibians we studied and particularly for PFOS. This result reflects a high potential for PFAS trophic transfer because amphibians are often low in trophic position and are important prey for many aquatic and terrestrial species., (Copyright © 2019. Published by Elsevier Inc.)

New surface initiators for ARGET ATRP (activators regenerated by electron transfer atomic transfer radical polymerization) have been prepared by the plasma deposition of haloester monomers. Specifically, methyl 3-bromopropionate (M3BP), methyl 2-chloropropionate, and ethyl 2-fluoropropionate (E2FP) were plasma deposited onto glass discs using RF glow discharge plasma. This technique creates surface coatings that are resistant to delamination and rich in halogen species making them good candidates for surface initiators for ARGET ATRP. Of all the plasma polymerized surface coatings, M3BP showed the highest halogen content and was able to grow 2-hydroxyethyl methacrylate (HEMA) polymer brushes on its surface via ARGET ATRP in as little as 15 min as confirmed by XPS. Surprisingly, E2FP, a fluoroester, was also able to grow HEMA polymer brushes despite fluorine being a poor leaving group for ARGET ATRP. The versatility of RF glow discharge plasma offers a clear advantage over other techniques previously used to immobilize ARGET ATRP surface initiators.

The Acinetobacter sp. strain M isolated from a contaminated soil sample in Jiangsu Province of China was found to be able to degrade perfluorooctane sulfonamide (PFOSA) effectively. Fluoride anion (F - ) released from PFOSA degradation was detected by ion chromatography, and showed positive correlation to the growth curve of Acinetobacter sp. strain M. The PFOSA degradation efficiency of strain M was approximately 27 %, as assessed by GC analysis. It was shown that enzymes localized outside of cells of Acinetobacter sp. strain M catalyzed the degradation of PFOSA. This further indicates a possibly new (multi-step/pathway) mechanism for PFOSA degradation. It revealed that the extracellular enzyme of the Acinetobacter strain M preferentially cleaves carbon-carbon and carbon-fluorine bonds instead of destroying the carbon-sulfur bond. The growth condition for Acinetobacter sp. strain M was optimized at 30 °C and pH 7.0 in the presence of 2000 mg L -1 of PFOSA and 0.5 % (v/v) of Tween-20. The optimal PFOSA degradation time was found to be 12 h, with a degradation efficiency of 76 % by extracellular enzymes in strain M as determined by GC analysis. The result may provide potential applications for biodegradition of perfluoro organic compounds, such as derivatives of perfluorooctane (C8)., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

This study investigated the adverse effects of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) on mouse primary hepatocytes by conducting cell viability, apoptosis, intracellular oxidative stress level, superoxide dismutase (SOD), catalase (CAT) activity and glutathione level assays. It was shown that PFOA and PFOS altered antioxidant enzymes activities and triggered oxidative stress, and thus exhibited cytotoxicity to the hepatocytes. Molecular mechanisms of SOD activities were measured and structural changes were explored by isothermal titration calorimetry and multiple spectroscopy. PFOA and PFOS bind to SOD via electrostatic forces with 7.634 ± 0.06 and 9.7 ± 0.4 sites, respectively, leading to structural and conformational changes. The overall results demonstrated that PFOS and PFOA are able to interact with SOD directly, resulting in producing oxidative stress and induce apoptosis., (Copyright © 2019 Elsevier Inc. All rights reserved.)

The concentrations of 17 perfluoroalkyl substances (PFASs) were investigated in paddy soil, void water, and brown rice collected from 30 paddy fields to examine the uptake of PFASs from soil by rice. The total concentrations of PFASs ranged from 7.76 to 3020 ng/L (average = 166 ng/L) in void water, 0.120 to 13.9 ng/g dry-weight (dw) (1.92 ng/g dw) in paddy soils, and from not-detected to 1.85 ng/g (0.403 ng/g) in brown rice samples. The highest PFAS concentrations were observed in brown rice cultivated in a paddy field where high levels of PFASs were observed in void water and paddy soil. Among target PFAS compounds, perfluorocarboxylic acids were dominant and detected in all matrices, and perfluorooctanoic acid (PFOA) was the most predominant compound in brown rice and void water. Significant positive correlations were examined for some detected PFASs between each matrix. PFOA in brown rice was positively correlated with PFOA in void water as well as perfluorodecanoic acid (PFDA) in paddy soil (p < 0.01). PFOA in void water also had correlated with PFDA in paddy soil. However, there was no correlation of other compounds between each matrix, except for correlations of perfluorononanoic acid (PFNA) and PFDA in paddy soil with those in void water, respectively (p < 0.05). Moreover, PFOA concentration in brown rice (0.093 ng/g) was much higher than one in white rice detected with a non-detectable level., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Introduction: Exposure to perfluoroalkyl substances (PFAS), synthetic and persistent chemicals used in commercial and industrial processes, are associated with cardiometabolic dysfunction and related risk factors including reduced birth weight, excess adiposity, and dyslipidemia. Identifying the metabolic changes induced by PFAS exposure could enhance our understanding of biological pathways underlying PFAS toxicity., Objective: To identify metabolic alterations associated with serum concentrations of four PFAS in children using a metabolome-wide association study., Methods: We performed untargeted metabolomic profiling by liquid chromatography with ultra-high-resolution mass spectrometry, and separately quantified serum concentrations of perfluorooctanoic acid, perfluorooctanesulfonic acid, perfluorononanoic acid, and perfluorohexanesulfonic acid (PFHxS) for 114 8-year old children from Cincinnati, OH. We evaluated associations between each serum PFAS concentration and 16,097 metabolic features using linear regression adjusted for child age, sex, and race with a false discovery rate < 20%. We annotated PFAS-associated metabolites and conducted pathway enrichment analyses., Results: Serum PFAS concentrations were associated with metabolic features annotated primarily as lipids and dietary factors. Biological pathways associated with all four PFAS included arginine, proline, aspartate, asparagine, and butanoate metabolism., Conclusions: In this cross-sectional study, childhood serum PFAS concentrations were correlated with metabolic pathways related to energy production and catabolism. Future studies should determine whether these pathways mediate associations between PFAS exposure and childhood cardiometabolic health.

Perfluoroalkyl acids (PFAAs) are a class of emerging pollutants. However, the bioconcentration and tissue distribution of shorter chain PFAAs in aquatic animals are not well understood. Here, we investigated the effects of perfluorinated carbon chain length of PFAAs and protein content of tissues on the bioconcentration and tissue distribution of both shorter chain PFAAs (linear C-F = 3-6) and longer chain PFAAs (linear C-F = 7-11) in zebrafish. The results showed that the uptake rate constants (k u ) and the bioconcentration factors (BCF ss ) of the shorter chain PFAAs (0.042-32 L·kg ww -1 ·d -1 and 0.12-24 L·kg ww -1 , respectively) in tissues were significantly lower than those of the longer chain PFAAs (2.8-1.4 × 10 3  L·kg ww -1 ·d -1 and 9.7-1.9 × 10 4  L·kg ww -1 , respectively). Moreover, the concentrations of both longer and shorter chain PFAAs were lowest in the muscle where the protein content was lowest, and they were highest in blood and liver where the protein content was highest among tissues except brain. The protein content of the brain was higher than that of the liver but the concentrations of PFAAs in the brain were significantly lower than those in the liver because of the blood-brain barrier. In addition, the ovary/blood and brain/blood ratios of concentrations for the shorter chain PFAAs were lower than those for the longer chain PFAAs. Generally, both log k u and log BCF ss showed a significantly positive correlation with either perfluorinated carbon number of PFAAs or protein content of tissues (P < 0.05). Further nonlinear surface fitting revealed that the effect of perfluorinated carbon number was more significant than protein content on the PFAA bioconcentration in zebrafish tissues. These results suggest that there are differences in the bioconcentration and tissue distribution between longer and shorter chain PFAAs and the shorter chain PFAAs seem to be safe compared with the longer chain PFAAs., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Eggs of snow buntings (Plectrophenax nivealis) were applied as a bio-indicator to examine differences in exposure to legacy persistent organic pollutants (POPs) and perflouroalkyl subtances (PFAS) from the terrestrial environment surrounding the settlements of Longyearbyen, Barentsburg and Pyramiden on Svalbard, Norway. Significantly higher concentrations of summed polychlorinated biphenyls (sumPCB7) in eggs collected from Barentsburg (2980 ng/g lipid weight (lw)) and Pyramiden (3860 ng/g lw) compared to Longyearbyen (96 ng/g lw) are attributed to local sources of PCBs within these settlements. Similar findings were observed for p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) where higher median concentrations observed in Pyramiden (173 ng/g lw) and Barentsburg (75 ng/g lw) compared to Longyearbyen (48 ng/g lw) may be influenced by guano inputs from breeding seabird populations, although other point sources cannot be ruled out. Concentrations of perfluorooctane sulphonate (PFOS) and several perfluorinated carboxylic acids (PFCAs) in snow bunting eggs were found to be statistically higher in the populated settlements of Longyearbyen and Barentsburg compared to the abandoned Pyramiden. Narrow foraging ranges of snow buntings during breeding season was useful in assessing point sources of exposure for PCBs and PFAS at particular sites with extreme differences observed between nest locations. SumPCB7 concentrations ranged from 2 μg/g ww to below detection limits between nest sites located less than a kilometer from each other in Pyramiden. Similar findings were observed in Longyearbyen, where several PFCAs ranged from 2 to 55 times higher between nest sites with similar spatial distances. These findings indicate that snow buntings can be a useful bio-indicator offering high spatial resolution for contaminant source apportionment in terrestrial environments on Svalbard., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Herein, we maximize the labeling efficiency of cardiac progenitor cells (CPCs) using perfluorocarbon nanoparticles (PFCE-NP) and 19 F MRI detectability, determine the temporal dynamics of single-cell label uptake, quantify the temporal viability/fluorescence persistence of labeled CPCs in vitro, and implement in vivo, murine cardiac CPC MRI/tracking that could be translatable to humans. FuGENE HD -mediated CPC PFCE-NP uptake is confirmed with flow cytometry/confocal microscopy. Epifluorescence imaging assessed temporal viability/fluorescence (up to 7 days [D]). Nonlocalized murine 19 F MRS and cardiac MRI studied label localization in terminal/longitudinal tracking studies at 9.4 T (D1-D8). A 4-8 fold 19 F concentration increase is evidenced in CPCs for FuGENE vs. directly labeled cells. Cardiac 19 F signals post-CPC injections diminished in vivo to ~31% of their values on D1 by D7/D8. Histology confirmed CPC retention, dispersion, and macrophage-induced infiltration. Intra-cardiac injections of PFCE-NP-labeled CPCs with FuGENE can be visualized/tracked in vivo for the first time with 19 F MRI., (Copyright © 2018 Elsevier Inc. All rights reserved.)

PFAAs have emerged as persistent organic pollutants (POPs) in a variety of environmental matrixes and biota, posing potential hazards for wildlife and humans. Diet has been considered as an important source for human exposure to PFAAs and seafood accounts for a relatively large proportion in human diets, especially for coastal residents. In an effort to clarify the impact of PFAAs in seafood on human health, 17 PFAAs were determined in 42 pooled seafood samples (14 species) from the Yellow Sea areas, China. The concentrations of total PFAAs (sum of 17 PFAAs, ∑PFAAs) were in the range of 1.10-1067 ng/g dry weight (dw), with the highest concentration found in swimming crab (Portunus trituberculatus) and the lowest in silvery pomfret (Pampus argenteus). Concentrations and composition profiles of PFAAs varied significantly among different species, suggesting that bioaccumulation potential of PFAAs differed from species to species. The distinct spatial distribution of PFAAs in four categories of seafood could be mainly attributed to the contamination patterns of PFAAs in three nearby cities. PFBA was presented as the most abundant PFAA in this study, which was different from the findings in many other studies where PFOS was the predominant compound. Furthermore, the human health risk assessment suggested that a comprehensive action plan is needed to protect people from high exposure to PFAAs through seafood consumption., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) have been reported to cause adverse health effects on wildlife as well as humans. Numerous studies demonstrated that PFOA and PFOS could interfere with the transcriptional activation of estrogen receptor α (ERα) by mimicking the function of endogenous ligand, whereas some reports suggested that the two compounds present non-estrogenic activities. These conflicting results bring a confusion to understand their molecular mechanism on the ERα-mediated signaling pathway. To address this issue, we performed the molecular docking and molecular dynamics simulations to elaborate the structural characteristics for the binding of PFOA and PFOS to ERα. Our results indicated that the two opposite binding orientations were modulated by the protonation states of key residue His524. In sub-acidic condition, PFOA and PFOS prefer to form the H-bonding interactions with the protonated His524, whereas Arg394 provided the H-bonding interactions for stable binding in sub-alkaline condition. Conformational analyses implied that the diverse binding modes were closely related to the conformational propensity of ERα for subsequent coactivator recruitment and transcription activation. Generally, our findings provide a flexible strategy to assess the pH impacts of microenvironment on the toxicities of perfluoroalkyl acids by their interactions with proteins., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Bacterial cytochrome P450s (P450s) are at the focus of attention as potential biocatalysts for applications in green synthetic chemistry, as they possess high activity for the hydroxylation of inert substrate C-H bonds. The high activity of bacterial P450s, such as P450BM3, is chiefly due to their high substrate specificity, and consequently, the catalytic activity of P450BM3 toward non-native substrates is very low, limiting the utility of bacterial P450s as biocatalysts. To enable oxidation of non-native substrates by P450BM3 without any mutagenesis, we have developed a series of "decoy molecules", inert dummy substrates, with structures that resemble those of the native substrates. Decoy molecules fool P450BM3 into generating the active species, so-called Compound I, enabling the catalytic oxidation of non-native substrates other than fatty acids. Perfluorinated carboxylic acids (PFCs) serve as decoy molecules to initiate the activation of molecular oxygen in the same manner as long-alkyl-chain fatty acids, due to their structural similarity, and induce the generation of Compound I, but, unlike the native substrates, PFCs are not oxidizable by Compound I, allowing the hydroxylation of non-native substrates, such as gaseous alkanes and benzene. The catalytic activity for non-native substrate hydroxylation was significantly enhanced by employing second generation decoy molecules, PFCs modified with amino acids (PFC-amino acids). Cocrystals of P450BM3 with PFC9-Trp revealed clear electron density in the fatty-acid-binding channel that was readily assigned to PFC9-Trp. The alkyl chain terminus of PFC9-Trp does not reach the active site owing to multiple hydrogen bonding interactions between the carboxyl and carbonyl groups of PFC9-Trp and amino acids located at the entrance of the substrate binding channel of P450BM3 that fix it in place. The remaining space above the heme after binding of PFC9-Trp can be utilized to accommodate non-native substrates. Further developments revealed that third generation decoy molecules, N-acyl amino acids, such as pelargonoyl-l-phenylalanine (C9-Phe), can serve as decoy molecules, indicating that the rationale "fluorination is required for decoy molecule function" can be safely discarded. Diverse carboxylic acids including dipeptides could now be exploited as building blocks, and a library of decoy molecules possessing diverse structures was prepared. Among the third-generation decoy molecules examined N-enanthyl-l-proline modified with l-phenylalanine (C7-Pro-Phe) afforded the maximum turnover rate for benzene hydroxylation. The structural diversity of third-generation decoy molecules was also utilized to control the stereoselectivity of hydroxylation for the benzylic hydroxylation of Indane, showing that decoy molecules can alter stereoselectivity. As both the catalytic activity and enantioselectivity are dependent upon the structure of the decoy molecules, their design allows us to regulate reactions catalyzed by wild-type enzymes. Furthermore, decoy molecules can also activate intracellular P450BM3, allowing the use of E. coli expressing wild-type P450BM3 as an efficient whole-cell bioreactor. It should be noted that Mn-substituted full-length P450BM3 (Mn-P450BM3) is also active for the hydroxylation of propane in which the regioselectivity diverged from that of Fe-P450BM3. The results summarized in this Account represent good examples of how the reactive properties of P450BM3 can be controlled for the monooxygenation of non-native substrates in vitro as well as in vivo to expand the potential of P450BM3.

Perfluoroalkyl substances (PFASs) are a family of synthetic, fluorinated organic compounds. They have been widely used in industrial applications and consumer products and widespread in the environment, wildlife and human. Experimental and epidemiologic evidence suggested that PFASs are capable of interfering with endocrine processes and have potential reproductive and developmental toxicities. Polycystic ovarian syndrome (PCOS), one of the main reasons of female infertility, is a common endocrine disorder in reproductive age women. We performed a case-control study to evaluate associations between PCOS-related infertility and PFASs concentrations in plasma. A total of 180 infertile PCOS-cases and 187 healthy controls were recruited from the Center for Reproductive Medicine of Shandong University. Blood specimens were collected at enrollment and analyzed for ten PFASs using liquid chromatography-tandem mass spectrometry. Multivariable logistic regression procedure was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for each PFAS. Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) were the dominant PFASs in the plasma of participants, with the median concentration of 5.07 ng/mL and 4.05 ng/mL, respectively. The median levels of individual PFAS were not significantly different between PCOS-cases and controls. While adjusted for the potential confounders (age, BMI, household income, education level, employment status, age at menarche, menstrual volume), the plasma concentration of perfluorododecanoic acid (PFDoA), a 12 carbons lengths of perfluorocarboxylic acids, was associated with a significantly increased risk of PCOS-related infertility (medium vs low tertile: OR = 2.36, 95% CI: 1.12, 4.99, P = 0.02; high vs low tertile: OR = 3.04, 95% CI: 1.19, 7.67, P = 0.02), with the P trend 0.01. No significant relationship was observed between PCOS-related infertility and other PFAS analytes in the adjusted model, despite perfluoroundecanoic acid showed a negative association (P trend 0.03). The potential reproductive health effects of PFASs and the underlying mechanisms merit further investigation in the future., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The bioavailability and bioaccumulation of sedimentary hydrophobic organic compounds (HOCs) is of concern at contaminated sites. Passive samplers have emerged as a promising tool to measure the bioavailability of sedimentary HOCs and possibly to estimate their bioaccumulation. We thus analyzed HOCs including organochlorine pesticides (OCPs), polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), and polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) in sediment, porewater, and river water using low-density polyethylene passive samplers and in 11 different finfish species and blue crab from the lower Passaic River. In addition, perfluorinated alkyl acids (PFAAs) were measured in grab water samples, sediment, and fish. Best predictors of bioaccumulation in biota were either porewater concentrations (for PCBs and OCPs) or sediment organic carbon (PBDEs and PFAAs), including black carbon (OCPs, PCBs, and some PCDD/F congeners)-normalized concentrations. Measured lipid-based concentrations of the majority of HOCs exceeded the chemicals' activities in porewater by at least 2-fold, suggesting dietary uptake. Trophic magnification factors were >1 for moderately hydrophobic analytes (log octanol-water partitioning coefficient [K OW ] = 6.5-8.2) with low metabolic transformation rates (<0.01 d -1 ), including longer alkyl chain PFAAs. For analytes with lower (4.5-6.5) and higher (>8.2) K OW s, metabolic transformation was more important in reducing trophic magnification. Environ Toxicol Chem 2019;38:872-882. © 2019 SETAC., (© 2019 SETAC.)

We investigated the profiles and levels of perfluoroalkyl carboxylic acid (PFCA) contamination in Pacific cod (Gadus macrocephalus) from the North Pacific Ocean. The mean concentrations of PFCAs containing 8 to 14 carbon atoms (C8-C14) in edible Pacific cod muscle ranged from 216 to 670 pg g -1 wet weight in the Northeast Pacific Ocean (Seattle, Vancouver, Alaska, and Russia), from 819 to 1710 pg g -1 wet weight in Japanese coastal waters (Hokkaido, Aomori, Iwate, Miyagi, Tottori, and Shimane), and from 288 to 892 pg g -1 wet weight in Korean waters (Sokcho, Busan, and Yeosu). These results indicate there are geographical differences in the distribution of PFCAs. The long-chain PFCAs (C9-C14) contributed 96% of the total PFCA concentration across Japan, whereas they contributed only 33% of the total PFCA concentration in the USA and Canada. Long-chain PFCA concentrations in cod samples collected in Japanese and Korean waters were about three to four times those in samples from the USA, Canada, and Russia. Because seafood is considered an important dietary source of PFCAs, high concentrations of long-chain PFCAs in Pacific cod from Japanese and Korean waters may affect human dietary exposure and blood concentrations of long-chain PFCAs., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

National Health and Nutrition Examination Survey 2007-2014 data (N = 6844) for adults aged ≥ 20 years were analyzed to estimate associations of perfluoroalkyl substances (PFAS), namely, PFOA, PFOS, PFDA, PFHxS, and PFNA with uric acid across stages of declining glomerular function. The population was stratified by the estimated glomerular filtration rates (eGFR) stages accompanying kidney disease: GF-1 with eGFR > 90 mL/min/1.73 m 2 ; GF-2 with eGFR 60-89 mL/min/1.73 m 2 ; GF-3A with eGFR 45-59 mL/min/1.73 m 2 ; and GF-3B/4 with eGFR 15-44 mL/min/1.73 m 2 . Adjusted and unadjusted geometric means of uric acid increased from GF-1 to GF-3B/4 for males and females. Adjusted geometric means for uric acid were higher for males by 1.38, 1.03, and 0.62 mg/dL for GF-1, GF2, and GF-3 respectively but for GF-3B/4, females had higher adjusted geometric means than males by 0.16 mg/dL, revealing narrowing of sex differences in uric acid as glomerular function declines. The direction of association between PFAS and uric acid was positive for GF-1 and GF-2 for males and for every PFAS except PFDA for females. For males for GF-3B/4, association between every PFAS except PFHxS and uric acid was found to be negative (p < 0.01). For females, only PFHxS actually reverses its relationship with increasing stages of renal disease. Uric acid associations with PFAS reverse in males with advanced renal failure. An implication is that previously reported association of PFAS exposure with uric acid is not due to renal failure. Understanding of other biomarkers associated with both PFAS exposure and renal failure may benefit from similar evaluation.

Sixteen per- and polyfluoroalkyl substances (PFASs) were measured in liver (n = 52) and kidney (n = 18) tissues of Indo-Pacific humpback dolphins (Sousa chinensis) stranded in the Pearl River Estuary (PRE) of China between 2004 and 2016. The average concentrations of perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) and most of other PFASs in the liver samples were respectively greater than any records previously reported in cetaceans globally. PFOS levels in 46% of dolphin liver samples exceeded the hepatic toxicity threshold in cetaceans. For the first time, we found a U-shaped trend for the distribution pattern of perfluorinated carboxylic acids (PFCAs) between liver and kidney with increasing carbon chain lengths (C5-C16), whereas a descending trend (C4-C10) was found for perfluoroalkane sulfonic acids (PFASs), which may be explained by binding efficiencies of PFAS analogues to proteins. Dolphins with the highest levels of ∑PFASs (age-corrected) were clustered near the river outlets in Lingdingyang area, which agrees with the spatial distribution of PFASs in the environment. Significant temporal trends were observed for many PFASs. Concentrations of PFOA, perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA) and perfluoroheptanoic acid (PFHpA) all peaked in year 2011, followed by a decreasing trend, while a consistently descending trend was shown for perfluoroundecanoic acid (PFUdA) and perfluorodecane sulfonate (PFDS). Our findings contribute to the knowledge of tissue distribution and spatiotemporal trends of PFASs in the PRE dolphins, which are valuable for us to understand the PFASs exposure risk and their industrial emission in Southern China., (Copyright © 2018. Published by Elsevier B.V.)

Perfluoroalkylated acids (PFAAs) are persistent chemicals that have been detected globally in the environment and in wildlife. Although it is known that PFAAs sorb to solid matrices, little is known on PFAA concentrations in soils. PFAA pollution has often been studied in aquatic invertebrates. However, this has rarely been done on terrestrial species. In the present study, we examined whether the concentrations of 15 PFAAs in isopods (Oniscidae), collected at a fluorochemical plant and in four other areas, representing a gradient in distance from the pollution source (1 km to 11 km), were related to those in the soil and in eggs of a songbird, the great tit (Parus major), collected in the same areas. Additionally, we examined the effect of physicochemical properties such as total organic carbon (TOC) and clay content on the relationship between the concentrations in soil and isopods. Finally, we examined the composition profile in the soil and isopods. Mean PFOS and PFOA concentrations of 1700 ng/g dw and 24 ng/g dw were detected in the soil at the plant. PFOS and PFPeA were the dominant PFAAs in isopods and were detected at mean concentrations of 253 and 108 ng/g ww, respectively. The great tit eggs showed elevated mean PFOS concentrations of 55,970 ng/g ww. In most cases, PFAA concentrations decreased with increasing distance from the plant. As PFAA concentrations in isopods were correlated with concentrations in the soils, isopods could serve as a bioindicator for PFAA concentrations in soils. Additionally, there were indications that isopods could also serve as a bioindicator for PFAA concentrations in eggs of great tits. However, these indications were only the case at two locations, showing the need to further monitor the possibilities of using isopods as a bioindicator for PFAA concentrations in song bird eggs. CAPSULE: Elevated PFAA concentrations in isopods reflected concentrations in songbird eggs and in soil, indicating that trophic transfer of PFAAs from soil, via isopods, to songbirds might play a role in the PFAA exposure of terrestrial songbirds., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Polyfluoroalkyl phosphates esters (PAPs) are widely used in a variety of commercial products, and have been detected in many aquatic organisms. In this study, common carps (Cyprinus carpio) were administered with 6:2 and 8:2 diPAP in water to investigate their bio-accumulation and transformation in fish. Several degradation products, including fluorotelomer unsaturated carboxylic acids (6:2 and 8:2 FTUCA), 5:3 and 7:3 fluorotelomer carboxylic acids (5:3 and 7:3 FTCA), perfluoroalkyl carboxylates (PFCAs) were identified in the carp liver. In addition, several phase-II metabolites, such as glutathione- and glucuronide-conjugated compounds were detected in the carp bile. 8:2 diPAP displayed lower accumulation potential than 6:2 diPAP probably due to its relatively large molecular size. However, 8:2 diPAP experienced more extensive transformation (transformation rate 6.78-14.6 mol%) and produced more phase I metabolites than 6:2 diPAP (0.49-0.66 mol%). The in vitro incubation with the liver S9 fraction confirmed that biotransformation of 6:2 and 8:2 diPAP took place in the carp liver. Further analyses of enzyme activities indicated that acid phosphatase (ACP) could be involved in mediating phase I while glutathione S-transferase (GST) involved in phase II metabolism of 6:2 and 8:2 diPAP in carp., (Copyright © 2018 Elsevier B.V. All rights reserved.)

As a result of their ubiquitous presence in the environment perfluoroalkyl acids (PFAAs) have been demonstrated in numerous organisms worldwide, in particular in those of higher trophic levels. The fact that PFAAs have been detected in various human matrices, together with the resultant potential human health risks, knowledge of possible paths of entry and distribution in various ecosystems, is of great importance. In this context pooled samples of terrestrial ecosystems - roe deer liver (n = 141), earthworms (n = 44) as well as leaves from beech and poplar trees (n = 70) - from the year 1989-2015 were obtained from the German Environmental Specimen Bank and examined for the presence of 11 PFAAs for the first time. In addition to determining individual and total PFAA concentrations, temporal trends have been deduced in order to determine the effectiveness of regulatory measures. The highest total mean concentration of PFAAs (sum of the concentrations of the 11 analytes) were 9.9 μg/kg in the roe deer liver samples, followed by earthworm samples with a mean PFAA concentration of 3.5 μg/kg and leaves with a mean total concentration of 2.5 μg/kg. In regard to temporal trends there was a significant reduction of concentrations for perfluorooctane sulfonate, perfluorooctanoic acid, perfluorononanoic acid and perfluorodecanoic acid in roe deer liver from 2003 to 2015, an indication of the effectiveness of regulatory measures. The same is true for the perfluorooctane sulfonate concentrations in earthworms and for perfluorooctanoic acid concentrations in the leaves. In contrast, an increase in perfluorobutanoic acid concentrations was observed from 2003 to 2015 in all three matrices. In summary it must be noted that in spite of the discernible effectiveness of minimization strategies, PFAAs are still detectable in terrestrial matrices and concentrations of other PFAAs as perfluorobutanoic acid appear to be increasing., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Organic compounds could be taken up by plants via different pathways, depending on chemical properties and biological species, which is important for the risk assessment and risk control. To investigate the transport pathways of perfluoroalkyl acids (PFAAs) by wheat (Triticum acstivnm L.), the uptake of five perfluoroalkyl carboxylic acids (PFCAs): TFA (C2), PFPrA (C3), PFBA (C4), PFHxA (C6), PFOA (C8), and a perfluoroalkyl sulfonic acid: PFOS (C8)) were studied using hydroponic experiments. Various inhibitors including a metabolic inhibitor (Na 3 VO 4 ), two anion channel blockers (9-AC, DIDS), and two aquaporin inhibitors (AgNO 3 , glycerol) were examined. The wheat root and shoot showed different concentration trends with the carbon chain length of PFAAs. The uptake of TFA was inhibited by Na 3 VO 4 and 9-AC whereas PFPrA was inhibited by Na 3 VO 4 , AgNO 3 and 9-AC. For the other four PFAAs, only Na 3 VO 4 was effective. These results together with the result of concentration-dependent uptake, which followed the Michaelis-Menten model, indicate that the uptake of PFAAs by wheat is mainly an energy-dependent active process mediated by carriers. For the ultra-short chain PFCAs (C2 and C3), aquaporins and anion channels may also be involved. A competition between TFA and PFPrA was determined during the plant uptake but no competition was observed between these two shorter chain analogues with other analogues, neither between PFBA and PFHxA, PFBA and PFBS, PFOA and PFOS., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Perfluorooctanoic acid (PFOA) has long been produced and widely used due to its excellent water and oil repellent properties. However, this trend has facilitated to the ubiquitous existence of PFOA in environmental matrix, and the potential ecotoxicity on aquatic organisms has not been fully elucidated. To study the tissue-specific bioconcentration and the nervous system- and energy-related biochemical effects of PFOA, as well as the phenotypic alterations by this chemical, male crucian carp (Carassius auratus) were exposed to gradient concentrations of PFOA (nominal 0.2, 10, 500 and 25,000 μg/L) in a flow-through apparatus for 7 days. PFOA was enriched in tissues following an order of blood > kidney ≥ liver > gill > brain > muscle. The bioconcentration factors ranged from 0.1 to 60.4. Acetylcholinesterase activity in the fish brain was inhibited, while liver carboxylesterase was induced in most cases and attenuated with time. The acyl-CoA oxidase activity was dose-dependently elevated and accompanied by a decline of ATP contents. PFOA treatments also inhibited the activity of the electron transport system (ETS). At the transcriptional level, ETS component complexes II and IV were concordantly depressed, and ATP synthesis was also downregulated. The mRNA level of peroxisome proliferator activated receptor α was increasingly upregulated, with related downstream genes upregulated in varying degrees. The phenotypes showed patterns of increased liver pathology and reduced swimming activity. In summary, PFOA leads to adverse effects in Carassius auratus related to multiple aspects, which may be associated with the nervous system, fundamental energy metabolism and other unpredictable factors. The results obtained in this study are expected to help clarify the PFOA toxic mechanisms on energy relevance., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Although bird eggs have been used in biomonitoring studies on perfluoroalkyl acids (PFAAs), effects of environmental concentrations on reproduction remain largely unknown in wild birds. In the present study we examined the associations between the concentrations of 4 perfluoroalkyl sulfonic acids (PFSAs) and 11 perfluoroalkyl carboxylic acids (PFCAs) in the eggs of great tits (Parus major), collected along a distance gradient from a pollution source, and multiple reproductive parameters (including the start of egg laying, clutch size, hatching success, fledging success and total breeding success) along with egg shell thickness and body condition of the nestlings. The PFAA concentrations measured at the plant site were among the highest ever reported in wild bird eggs. PFAA concentrations decreased sharply with increasing distance (0-11 km) from the plant, but remained relatively elevated in the adjacent sites. PFAAs were grouped into principal components (PCs) to prevent collinearity. High concentrations of PFOS, PFDS, PFDoDA, PFTrDA and PFTeDA (grouped as PC1) were associated with a reduced hatching success of nests where at least one egg hatched, thinner egg shells and increased survival of the hatched chicks. High concentrations of PFDA (PC2) were associated with a reduced hatching success, especially in nests where no eggs hatched, an earlier start of egg laying and a reduction of total breeding success, mainly caused by the failure in hatching. Although the major manufacturer of PFAAs phased out the production of perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) and related products in 2002, concentrations appear to have increased since previous measurements. Surprisingly, despite the very high concentrations close to the fluorochemical plant, there was no clear evidence for reproductive impairment as the observed associations between PFAA concentrations and reproductive parameters were rather limited compared to previous studies in songbirds. These findings also suggest potential differences in sensitivity between species. CAPSULE: Despite the very high PFAA concentrations at the perfluorochemical hotspot, correlations with reproductive parameters were limited., (Copyright © 2018 Elsevier B.V. All rights reserved.)

In this study, eggs from free-range and barn chickens in farms around a fluorochemical facility were collected to assess the distribution profiles of perfluoroalkyl acids (PFAAs), including isomers of perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), and perfluorohexane sulfonate (PFHxS), in egg yolk and albumen. The results revealed that the concentrations of PFAAs in yolks were significantly higher than those in albumen. All 17 PFAAs examined could be detected in yolks, showing decreasing concentrations with increasing distance from the fluorochemical facility. The three predominant compounds in yolks were perfluorobutanoic acid (PFBA, mean concentration 81.4 ng/g ww), PFOS (28.0 ng/g ww), and PFOA (4.83 ng/g ww), and this result is consistent with the product structure of the facility. Moreover, n-PFOA, n-PFOS, and n-PFHxS were the dominant contaminants in yolk, with mean concentrations of 4.75, 25.7, and 4.29 ng/g ww, respectively. In albumen, PFBA was still the predominant PFAA congener (mean concentration = 3.93 ng/g ww), followed by PFOA. Docking analysis indicated that the PFAAs presented higher binding abilities with the low density lipoprotein, high density lipoprotein, and vitellin proteins in yolk than that with ovalbumin albumen proteins, which might be the main factor influencing the possible difference in distributions of PFAAs in yolk and albumen., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Perfluorooctane sulfonyl fluoride (PFOSF) has been defined as persistent organic pollutant in the Stockholm Convention in 2009. Currently PFOSF and its substitutes (structural analogues in which octyl group is substituted for other aliphatic chains) are still scarcely studied. HSA is a main carrier for drugs in blood, and the influence of exogenous compounds including pollutants on HSA is of particular interest. In this work, the binding sites of HSA to Perfluoroalkane sulfonyl fluoride (PFASFs) were determined by fluorescence technique. The results demonstrated that PFASFs competitively bind to HSA at Sudlow's site I against warfarin and at Sudlow's site II against dansyl-proline and the related association constants were determined. The association constants of PFOSF, perfluorohexane sulfonyl fluoride (PFHSF) and perfluorobutane sulfonyl fluoride (PFBSF) were determined to be 2.59 × 10 -3  μM -1 , 4.65 × 10 -3  μM -1 and 2.85 × 10 -3  μM -1 at Sudlow's site I and 8.68 × 10 -4  μM -1 , 3.43 × 10 -2  μM -1 and 1.92 × 10 -2  μM -1 at Sudlow's site II, respectively. The results showed that PFASFs can bind tightly to HSA and thus migrate to all parts of the body through vascular system. Non-covalent interaction between HSA and PFASFs was confirmed with tryptic digestion experiment. The mass spectra results indicated that other binding sites of HSA are also involved in the binding of PFHSF and PFBSF. The total binding numbers of PFOSF, PFHSF and PFBSF on HSA are 2, 6 and 3, respectively., (Copyright © 2018 Elsevier B.V. All rights reserved.)

PFASs are a class of compounds that include perfluoroalkyl and polyfluoroalkyl substances, some of the most persistent pollutants still allowed - or only partially restricted - in several product fabrications and industrial applications worldwide. PFASs have been shown to interact with blood proteins and are suspected of causing a number of pathological responses, including cancer. Given this threat to living organisms, we carried out a broad review of possible sources of PFASs and their potential accumulation in agricultural plants, from where they can transfer to humans through the food chain. Analysis of the literature indicates a direct correlation between PFAS concentrations in soil and bioaccumulation in plants. Furthermore, plant uptake largely changes with chain length, functional group, plant species and organ. Low accumulations of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) have been found in peeled potatoes and cereal seeds, while short-chain compounds can accumulate at high levels in leafy vegetables and fruits. Significant variations in PFAS buildup in plants according to soil amendment are also found, suggesting a particular interaction with soil organic matter. Here, we identify a series of challenges that PFASs pose to the development of a safe agriculture for future generations., (Copyright © 2018 Elsevier Inc. All rights reserved.)

A suite of perfluoroalkyl substances (PFASs) and polychlorinated naphthalenes (PCNs) were determined in 41 peregrine falcon eggs collected in South Greenland between 1986 and 2014. Median concentrations of perfluorinated sulfonic acids (ΣPFSA) and perfluorinated carboxylic acids (ΣPFCA) were 303 ng/g dry weight (dw) (58 ng/g wet weight, ww) and 100 ng/g dw (19 ng/g ww), respectively, which was comparable to other studies. Perfluorooctane sulfonate (PFOS) accounted for 94% on average of all PFSAs, but did not show a significant time trend. Perfluorohexane sulfonate (PFHxS), perfluoroheptane sulfonate (PFHpS) and perfluorodecane sulfonate (PFDS) showed non-linear decreases over the study period, while some long-chain PFCAs increased significantly. The PCN profile was dominated by the penta-, hexa- and tetrachlorinated congeners CN-52/60, CN-66/67 and CN-42. CN-54, an indicator of combustion, accounted for 2.4% of ΣPCN on average. All PCN congeners showed a decreasing tendency, which was significant for lipid-normalized concentrations of CN-53, CN-54 and CN-63. The ΣPCN median concentration was 21 ng/g lipid weight, which is in the high end of concentrations reported for bird eggs. The PCN and PFAS concentrations add to an already high contaminant burden and a complex chemical cocktail in the peregrine falcon population in Greenland, mainly reflecting contaminant exposure during migration and winter stays in Central and South America., (Copyright © 2018 Elsevier B.V. All rights reserved.)

6:2 fluorotelomer sulfonamidoalkyl betaine (6:2 FTAB) is a major component of aqueous film-forming foams (AFFFs) used for firefighting and is frequently detected, along with one of its suspected transformation products, 6:2 fluorotelomer sulfonate (6:2 FTSA), in terrestrial and aquatic ecosystems impacted by AFFF usage. Biochemical processes underlying bacterial biodegradation of these compounds remain poorly understood due to a lack of pure culture studies. Here, we characterized the water-soluble and volatile breakdown products of 6:2 FTSA and 6:2 FTAB produced using Gordonia sp. strain NB4-1Y cultures over seven days under sulfur-limited conditions. After 168 h, 99.9% of 60 μM 6:2 FTSA was degraded into ten major breakdown products, with a mol% recovery of 88.2, while 70.4% of 60 μM 6:2 FTAB was degraded into ten major breakdown products, with a mol% recovery of 84.7. NB4-1Y uses two pathways for 6:2 FTSA metabolism, with 55 mol% of breakdown products assigned to a major pathway and <1.0 mol% assigned to a minor pathway. This work indicates that rapid transformation of 6:2 FTSA and 6:2 FTAB can be achieved under controlled conditions and improves the bacterial metabolism of these compounds., (Copyright © 2018 Elsevier B.V. All rights reserved.)

With regulations on the manufacture and usage of perfluorooctanoate (PFOA), perfluorooctane sulfonate (PFOS) and related compounds, short-chain perfluoroalkyl acids (PFAAs) are increasingly being used as alternatives. However, there are limited studies on their bioaccumulation mechanisms, especially for short-chain PFAAs. In this study, we examined the binding affinity of PFAAs with fish serum proteins and tissue distributions of perfluoroalkyl carboxylates (C7-C11 PFCAs) and perfluoroalkyl sulfonates (C4, C6, and C8 PFSAs) in carp (Cyprinus carpio), including the isomers of PFOS and perfluorohexane sulfonate (PFHxS). For both PFCAs and PFSAs, the fish serum protein binding constant (K A ) and bioconcentration factor (BCF) increased with an increase in the carbon chain length. PFHxS (C6 PFSA) had a much higher K A but displayed a much lower BCF than those of C7-C11 PFCAs. It indicated that not only fish blood proteins, but also other proteins in the liver and kidney, mediated the accumulation of PFAAs in fish. The lowest concentration ratios of PFHxS in liver to blood and in kidney to blood suggested that it could not be effectively transported to liver and kidney by fatty acid binding proteins and organic anion transporters. PFOS and PFHxS displayed different elimination pathways, although their linear (n-) isomers were accumulated more in fish than the corresponding branched (br-) isomers. The n-PFOS was eliminated more via the feces but br-PFOS was eliminated more via the urine; while the opposite trend was observed for PFHxS isomers., (Copyright © 2018 Elsevier B.V. All rights reserved.)

A field study was conducted to elucidate distribution and partitioning of perfluoroalkyl carboxylic acids (PFCAs; C7-12) in a terrestrial ecosystem that was contaminated with industrial sources of release. Surface soil (0-6 cm), plants, and earthworms were collected from a field located within a 1-mile radius of a fluoropolymer industry that had been manufacturing fluorochemicals for over five decades. The mean concentrations of ∑PFCAs were 150, 420, 61, 68, and 430 ng/g dry weight (dw) in surface soil, earthworms, grass roots, grass leaves, and tree leaves, respectively. The measured concentrations were higher than those reported for corresponding matrices in other locations worldwide, suggesting that fluorochemical manufacturing operations have contributed to the contamination of this nearby land. Soil and plant tissues consisted mainly of perfluorooctanoic acid (PFOA; C8) (77.3-97.1% of the total PFCAs), whereas longer-chain PFCAs, such as perfluoroundecanoic acid (PFUnDA; C11: 17.6%) and perfluorododecanoic acid (PFDoDA; C12: 31.9%), accounted for relatively higher proportions in earthworms. Spatial distribution of PFCAs at this site suggested that both atmospheric deposition and groundwater recharge have contributed to the sources of contamination. Both earthworm- and grass-accumulated PFCAs from soil with biota-soil accumulation factors and root concentration factors increased with perfluorocarbon chain length. The translocation factors of PFCAs in grass decreased as the number of carbons in the fluorocarbon moiety increased. This field study is appropriate to improve our understanding of partitioning of PFCAs among soil, plants, and earthworms., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) can be transferred from a mother to her fetus during pregnancy and adversely affect fetal development. However, the efficiency and influencing factors of PFASs maternal-fetal transfer remain unclear. We measured the levels of six perfluoroalkylcarboxylates, three perfluoroalkylsulfonates, and one sulfonamide in 369 pairs of maternal and umbilical cord serum and examined the transplacental transfer efficiency (TTE) of PFASs by the functional group and carbon chain length in a prospective birth cohort in Shandong, China. All ten PFASs were detected in both maternal and umbilical cord serum in nearly all samples. Maternal and cord levels were closely correlated (the correlation coefficient [r] ranging from 0.485 to 0.908) in most PFASs except perfluorobutane sulfonic acid (PFBS) (r = 0.159). TTE was significantly affected by the functional group and carbon chain length. Compared to perfluoroalkylcarboxylates, perfluoroalkylsulfonates had a lower ratio of maternal to fetal transfer. A U-shaped relationship between carbon chain length and TTE was observed for perfluoroalkylcarboxylates while a monotonic descending trend was identified between TTE and the increasing carbon chain length for perfluoroalkylsulfonates. PFASs can readily pass through the placenta. The functional group and carbon chain length are important determinants for the TTE of PFASs.

Key Message: Two lines of Taxus × media hairy roots harbouring or not the TXS transgene demonstrated diverse gene expression and taxane yield during cultivation in PFD-supported two liquid-phase culture system. Two lines of Taxus × media hairy roots were subjected to single or twice-repeated supplementation with methyl jasmonate, sodium nitroprusside, L-phenylalanine, and sucrose feeding. One line harboured transgene of taxadiene synthase (ATMA), while the second (KT) did not. Both hairy root lines were cultured in two-phase culture systems containing perfluorodecalin (PFD) in aerated or degassed form. The relationship between TXS (taxadiene synthase), BAPT (baccatin III: 3-amino, 3-phenylpropanoyltransferase), and DBTNBT (3'-N-debenzoyl-2-deoxytaxol-N-benzoyltransferase) genes and taxane production was analysed. The ATMA and KT lines differed in their potential for taxane accumulation, secretion, and taxane profile. In ATMA biomass, both paclitaxel and baccatin III were detected, while in KT roots only paclitaxel. The most suitable conditions for taxane production for ATMA roots were found in single-elicited supported with PFD-degassed cultures (2 473.29 ± 263.85 µg/g DW), whereas in KT roots in single-elicited cultures with PFD-aerated (470.08 ± 25.15 µg/g DW). The extracellular levels of paclitaxel never exceeded 10% for ATMA roots, while for KT increased up to 76%. The gene expression profile was determined in single-elicited cultures supported with PFD-degassed, where in ATMA roots, the highest taxane yield was obtained, while in KT the lowest one. The gene expression pattern in both investigated root lines differed substantially what resulted in taxane yield characterized particular lines. The highest co-expression of TXS, BAPT and DBTNBT genes noted for ATMA roots harvested 48 h after elicitation corresponded with their higher ability for taxane production in comparison with the effects observed for KT roots.

Although people are exposed daily to per- and polyfluorinated alkyl substances (PFASs), the biological consequences are poorly explored. The health risks associated with PFAS exposure are currently based on chemical analysis with a weak correlation to potential harmful effects in man and animals. In this study, we show that perfluorooctane sulfonic acid (PFOS), often the most enriched PFAS in the environment, can be transferred via bacteria to higher organisms such as Caenorhabditis elegans. C. elegans nematodes were exposed to PFOS directly in buffer or by feeding on bacteria pretreated with PFOS, and this led to distinct gene expression profiles. Specifically, heavy metal and heat shock associated genes were significantly, although inversely, expressed following the different PFOS exposures. The innate immunity receptor for microbial pathogens, clec-60, was shown for the first time to be down-regulated by PFOS. This is in line with a previous study indicating that PFOS is associated with children's susceptibility to certain infectious diseases. Furthermore, bar-1, a gene associated with various cancers was highly up-regulated only when C. elegans were exposed to PFOS pretreated live bacteria. Furthermore, dead bacterial biomass had higher binding capacity for linear and isomeric PFOS than live bacteria, which correlated to the higher levels of PFOS detected in C. elegans when fed the treated E. coli, respectively. These results reveal new aspects concerning trophic chain transport of PFOS., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Perfluorooctanesulfonate (PFOS) is a toxic and persistent organic pollutant that can be widely detected in agricultural soils. In this study, two lettuce cultivars with low PFOS accumulation were screened out to reduce the exposure of PFOS to the human body via vegetable consumption. The screened low-PFOS cultivars may help to ensure food safety, despite planting in highly PFOS-polluted soils (1.0 mg/kg), due to their high tolerance to PFOS and 4.4-5.7 times lower shoot PFOS concentration than the high-PFOS cultivars. Protein content and protein-mediated transpiration played key roles in regulating PFOS accumulation in the lettuce cultivars tested. Lower protein content, lower stomatal conductance, and lower transpiration rate resulted in low PFOS accumulation. This study reveals the mechanism of forming low-PFOS accumulation of lettuce cultivars at physiological and biochemical levels and lays a foundation for developing a cost-effective and safe approach to grow vegetables in PFOS-polluted soils.