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6,613 results on '"He, Chuan"'

201. Epitranscriptional regulation of TGF-[beta] pseudoreceptor BAMBI by [m.sup.6]A/YTHDF2 drives extrinsic radioresistance

Author

Wang, Liangliang, Si, Wei, Yu, Xianbin, Piffko, Andras, Dou, Xiaoyang, Ding, Xingchen, Bugno, Jason, Yang, Kaiting, Wen, Chuangyu, Zhang, Linda, Chen, Dapeng, Huang, Xiaona, Wang, Jiaai, Arina, Ainhoa, Pitroda, Sean, Chmura, Steven J., He, Chuan, Liang, Hua Laura, and Weichselbaum, Ralph

Subjects
Metastasis -- Diagnosis -- Care and treatment, Activins -- Analysis, Immunotherapy -- Patient outcomes, Bone morphogenetic proteins -- Analysis, Radiotherapy -- Patient outcomes, Health care industry
Abstract

Activation of TGF-[beta] signaling serves as an extrinsic resistance mechanism that limits the potential for radiotherapy. Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) antagonizes TGF-[beta] signaling and is implicated in cancer progression. However, the molecular mechanisms of BAMBI regulation in immune cells and its impact on antitumor immunity after radiation have not been established. Here, we show that ionizing radiation (IR) specifically reduces BAMBI expression in immunosuppressive myeloid-derived suppressor cells (MDSCs) in both murine models and humans. Mechanistically, YTH N6-methyladenosine RNA-binding protein F2 (YTHDF2) directly binds and degrades Bambi transcripts in an [N.sup.6]-methyladenosine-dependent ([m.sup.6]A-dependent) manner, and this relies on NF-[kappa]B signaling. BAMBI suppresses the tumor-infiltrating capacity and suppression function of MDSCs via inhibiting TGF-[beta] signaling. Adeno-associated viral delivery of Bambi (AAV-Bambi) to the tumor microenvironment boosts the antitumor effects of radiotherapy and radioimmunotherapy combinations. Intriguingly, combination of AAV-Bambi and IR not only improves local tumor control, but also suppresses distant metastasis, further supporting its clinical translation potential. Our findings uncover a surprising role of BAMBI in myeloid cells, unveiling a potential therapeutic strategy for overcoming extrinsic radioresistance., Introduction Radiation therapy (RT) is a common treatment option for cancer patients (1). Radiation simultaneously induces biological effects on cancer cells and elicits an immune response (2). However, the immunogenic [...]

Published
2023
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219. Direct DNA crosslinking with CAP-C uncovers transcription-dependent chromatin organization at high resolution

Author

You, Qiancheng, Cheng, Anthony Youzhi, Gu, Xi, Harada, Bryan T, Yu, Miao, Wu, Tong, Ren, Bing, Ouyang, Zhengqing, and He, Chuan

Subjects
Biochemistry and Cell Biology, Biological Sciences, Bioengineering, Genetics, Stem Cell Research, Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, Animals, CCCTC-Binding Factor, Chromatin, Cross-Linking Reagents, DNA, DNA (Cytosine-5-)-Methyltransferases, Enzyme Inhibitors, Genome, Mice, Mouse Embryonic Stem Cells, Nucleic Acid Conformation, Promoter Regions, Genetic, Transcription, Genetic
Abstract

Determining the spatial organization of chromatin in cells mainly relies on crosslinking-based chromosome conformation capture techniques, but resolution and signal-to-noise ratio of these approaches is limited by interference from DNA-bound proteins. Here we introduce chemical-crosslinking assisted proximity capture (CAP-C), a method that uses multifunctional chemical crosslinkers with defined sizes to capture chromatin contacts. CAP-C generates chromatin contact maps at subkilobase (sub-kb) resolution with low background noise. We applied CAP-C to formaldehyde prefixed mouse embryonic stem cells (mESCs) and investigated loop domains (median size of 200 kb) and nonloop domains (median size of 9 kb). Transcription inhibition caused a greater loss of contacts in nonloop domains than loop domains. We uncovered conserved, transcription-state-dependent chromatin compartmentalization at high resolution that is shared from Drosophila to human, and a transcription-initiation-dependent nuclear subcompartment that brings multiple nonloop domains in close proximity. We also showed that CAP-C could be used to detect native chromatin conformation without formaldehyde prefixing.

Published
2021

231. YTHDF2 inhibition potentiates radiotherapy antitumor efficacy

Author

Wang, Liangliang, Dou, Xiaoyang, Chen, Shijie, Yu, Xianbin, Huang, Xiaona, Zhang, Linda, Chen, Yantao, Wang, Jiaai, Yang, Kaiting, Bugno, Jason, Pitroda, Sean, Ding, Xingchen, Piffko, Andras, Si, Wei, Chen, Chao, Jiang, Hualiang, Zhou, Bing, Chmura, Steven J., Luo, Cheng, Liang, Hua Laura, He, Chuan, and Weichselbaum, Ralph R.

Published
2023
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236. United test of the equivalence principle at $10^{-10}$ level using mass and internal energy specified atoms

Author

Zhou, Lin, He, Chuan, Yan, Si-Tong, Chen, Xi, Duan, Wei-Tao, Xu, Run-Dong, Zhou, Chao, Ji, Yu-Hang, Barthwal, Sachin, Wang, Qi, Hou, Zhuo, Xiong, Zong-Yuan, Gao, Dong-Feng, Zhang, Yuan-Zhong, Ni, Wei-Tou, Wang, Jin, and Zhan, Ming-Sheng

Subjects
Quantum Physics, General Relativity and Quantum Cosmology, Physics - Atomic Physics
Abstract

We use both mass and internal energy specified rubidium atoms to jointly test the weak equivalence principle (WEP). We improve the four-wave double-diffraction Raman transition method (FWDR) we proposed before to select atoms with certain mass and angular momentum state, and perform dual-species atom interferometer. By combining $^{87}$Rb and $^{85}$Rb atoms with different angular momenta, we compare the differential gravitational acceleration of them, and determine the value of E\"{o}tv\"{o}s parameter, $\eta$, which measures the strength of the violation of WEP. For one case ($^{87}$Rb$|\emph{F}=1\rangle$ - $^{85}$Rb$|\emph{F}=2\rangle$),the statistical uncertainty of $\eta$ is $1.8 \times 10^{-10}$ at integration time of 8960 s. With various systematic errors correction, the final value is $\eta=(-4.4 \pm 6.7) \times 10^{-10}$. Comparing with the previous WEP test experiments using atoms, this work gives a new upper limit of WEP violation for $^{87}$Rb and $^{85}$Rb atom pairs., Comment: 5 pages, 3 figures

Published
2019
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