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When circularly polarized light travels through a ferrofluid film in the presence of an applied magnetic field, the transmitted light will behave as elliptically polarized light, because of magnetic birefringence and dichroism. The angular distribution of the relative intensity of the transmitted light can be obtained using a polarizer, so that the ratio of both the long and short axes—as well as the orientation angle of the ellipse, corresponding to the direction of the applied magnetic field—can be determined, and whether the ferrofluids samples are stable during the measurement can be directly judged from the shape of the distribution curves. Thus, the ratio of the amplitudes Ax/Ay and the added phase difference Δτ can be resolved in the elliptically polarized light, and information on both the magnetic birefringence Δn and the dichroism Δk can be deduced for the ferrofluid sample. From the orientation angles of both right-handed and left-handed elliptically polarized transmitted light, the direction of the applied magnetic field can be accurately determined. Using circularly polarized light, the magnetic birefringence and dichroism of pure γ-Fe2O3 ferrofluids and γ-Fe2O3/ZnFe2O4 binary ferrofluids were studied. For the binary ferrofluids, a modulating effect on the magnetic birefringence and dichroism was revealed.

In this study, γ-Fe2O3 ferrimagnetic nanoparticles and paramagnetic nanoparticles of p-MgFe2O4 (a hydroxide precursor for the preparation of magnesium ferrite materials) are produced by chemical precipitation technology. The γ-Fe2O3 ferrofluids and p-MgFe2O4 paramagnetic fluids are synthesised by Massart's method. The binary ferrofluids are obtained by mixing the ferrofluids and the paramagnetic fluids. There is insufficient magnetic interaction to aggregate the γ-Fe2O3 ferrimagnetic system and the p-MgFe2O4 paramagnetic system, so the magnetisation behaviour of the binary ferrofluids can be explored with reference to those of the single fluids. The magnetisation behaviour of single γ-Fe2O3 ferrofluids may be described by a model of gas-like compression. In the absence of a magnetic field, some particles can self-assemble into aggregates with a closed ring-like structure which make no contribution to the magnetisation of the γ-Fe2O3 ferrofluids. These ring-like aggregates result in the measured saturation...

The thermal aging experiments on nitrile rubber and rubber additives were carried out for different time in 25# transformer oil at 70°C. The amount of CH4 released from non-rubber group, nitrile rubber group and rubber additives group were measured by Gas Chromatography (GC). The molecular structure of NBR matrix and liquid extracted from NBR were analyzed with fourier transform infrared analysis (FTIR), respectively. At last, the formation mechanism of CH4 in nitrile rubber were investigated. The results illustrated that the rubber additive RD released methyl radicals and hydrogen radicals, and generated CH4 by reacted with each other.

This paper presents a mold cavity surface subdivision method by using image boundary detection technology to realize high efficiency NC machining. From the new perspective of image processing, the method constructed an optimal cutting direction angle field image in the u-v system of parameters according to the relationship between maximum cutting bandwidth and cutting direction in the theory of iso-scallop height firstly, and then fuzzy c - means clustering method was applied for subdivision surface boundary detection. Experimental results demonstrate good performance of the proposed method in distinguishing and extracting boundary points of surface subdivision.

Bacillus subtilis B47 is an endophytic bacterium of tomato, and produces substance that strongly inhibits the growth of Bipolaris maydis, the pathogen of southern corn leaf blight (SCLB), as well as several other phytopathogenic fungi. The antifungal substance was purified from the broth culture of the bacterium using acid precipitation, methanol extraction, and three-step chromatography. Based on FT-IR spectrometry, amino acid composition, and MALDI-TOF-MS/MS CID analyses, the antifungal substance was identified as iturin A2, a cyclic lipopeptide antibiotic. To evaluate the efficacy of iturin A2 for control of SCLB, partially purified iturin A2 (75%, w/v) was applied under different conditions. At the concentration of 300 mg kg-1, iturin A2 showed efficacy ranging from 100 to 53.1% under in vitro, in plot and in field conditions. This efficacy was higher than or similar to that of the fungicide chlorothalonil. When the concentration of iturin A2 was increased to 500 mg kg-1, the control efficacy was enhanced to 64.2% in field, which was significantly higher than that of chlorothalonil. These results indicate that iturin A2 has potential for SCLB control and could be a substitute to synthetic fungicides. To our knowledge, this is the first report on using partially purified iturin A for control of SCLB under field conditions.

In this paper fuzzy logic control is introduced in auto adjust control system of air in bus. Fuzzy controller employs fuzzy control model of two inputs and one output. The paper also introduces the hardware framework, working principle and software of the system, analyzing merits of fuzzy logic technique, putting emphasis on the design of the fuzzy controller. In the controlling process, fuzzy controller samples temperature signal in bus and calculate deflection and deflection rate of temperature, looks up corresponding output in controlling table, and calculates its precision, then fuzzy controller gives related signal to motors and valve, acquiring satisfactory effect.

With the rapid development of the global economy, the vehicle population increases rapidly year by year, but the traffic accidents have also increased. The vehicle population in China only accounts for 2-3% of the world at present, but the death is about 20% of the world. China has become the world's most serious national in the traffic accidents. In order to make the traffic accident rescue convenient, the automatic asking for help system is studied, and the software and hardware are also designed. The control system uses the PIC18F458 as a controller, which combines with the modern sensor technology and GPRS, and realizes the automatic asking for help in traffic accidents. Experiments show that the system has the advantages of stability, reliability and strong anti-interference ability.

With the rapid development of the global economy, automobile industry and high-grade highway create the necessary tools and infrastructure for the fast and convenient traffic. However, the cars also bring some negative effects of human life, such as traffic accidents. China has become the world's most serious national in the traffic accidents. In order to make the traffic accident rescue convenient, the automatic positioning system is studied, and the software and hardware are also designed. The control system uses the PIC18F458 as a controller, which combines with the modern sensor technology and GPS, and realizes the automatic positioning in traffic accidents. Experiments show that the system has the advantages of stability, reliability and strong anti-interference ability.

In this paper, three-dimensional automatic fuel measuring device of diesel engine test bed was created. The function of virtual assembly was achieved using virtual reality modeling language. Network virtual assembly can operate, browse and assemble automatic fuel measuring device remotely by embedding virtual assembly in web pages. The virtual assembly method presented in this paper can be easily extended to deal with many other problems in engineering system if control parameters are modified properly.

A series of substituted imidazo[1,2-a]pyrazin-8-amines were discovered as novel breast tumor kinase (Brk)/protein tyrosine kinase 6 (PTK6) inhibitors. Tool compounds with low-nanomolar Brk inhibition activity, high selectivity towards other kinases and desirable DMPK properties were achieved to enable the exploration of Brk as an oncology target.

In this paper, γ-Fe2O3 nanoparticles have been prepared by treating the hydroxide precursor in FeCl2 solution. A new transition process induced chemically for preparing oxide nanoparticles is revealed by energy dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction and electron microscopy. The precursor, consisting of a mixture of FeOOH and Mg(OH)2, was synthesized using a common co-precipitation method. After treatment by boiling in FeCl2 solution, γ-Fe2O3 nanoparticles, about 10 nm diameter, were formed. The nanoparticles are highly crystalline and the specific saturation magnetization σs reaches 69.20 emu g−1, which is 90 percent of the value for bulk γ-Fe2O3 and is higher than the value for γ-Fe2O3 nanoparticles of the same size prepared by other methods. The nanoparticles have an inert FeCl2 surface, giving them relatively good chemical stability and dispersivity.

In this paper, the thinning rate and die elastic deformation under large forming force are investigated by finite element simulation with experimental validation focused on the problem of coloring rate between die and panel in automotive die manufacturing. The study suggests that when the panel is formed, the thinning rate of panel has little influence on the coloring rate and the die elastic deformation under large forming force is the main influence factor. The contact pressure of die face and punch surface displacement distribution under forming force are got with simulation. With these results the method that die face is compensated reasonably is also proposed. The industrial application shows that the method can reduce the die polishing workloads obviously compared to the traditional method of die face equivalent offset. This paper has a guide for comprehensive understanding the influence factors of coloring rate and greatly reducing the die tryout period.

The competitive adsorption properties of the heavy metal ions Pb(II), Cu(II), and Cd(II) ions in binary and ternary systems on black carbon (BC) isolated from the burning residue of wheat straw were studied in batch systems. The BC samples were characterized by Fourier transform infrared spectroscopy (FTIR). Equilibrium and kinetic adsorption data showed that the selectivity of metal ions on BC followed the order of Pb(II) > Cu(II) > Cd(II). Kinetic studies were performed, and the rate kinetics was fitted well with the pseudosecond-order model. The Freundlich adsorption isotherm was applicable to the adsorption process, and its constants were evaluated.

In this paper, the thinning rate and die elastic deformation under large forming force are investigated by finite element simulation with experimental validation focused on the problem of coloring rate between die and panel in automotive die manufacturing. The study suggests that when the panel is formed, the thinning rate of panel has little influence on the coloring rate and the die elastic deformation under large forming force is the main influence factor. The definition of high contact pressure area is proposed by the comparison between the simulation and industrial experiment results. The definition greatly benefits the predication of die face coloring rate. The method that die face is compensated reasonably is also proposed. It can reduce the die polishing workloads obviously compared to the traditional method of die face equivalent offset. This paper has a guide for comprehensive understanding the influence factors of coloring rate and greatly reducing the die tryout period.

The kinetics of Pb (II) adsorption onto black carbon (BC) has been investigated. The BC samples were characterized by the techniques of X-ray diffraction (XRD), scanning electron microscope equipped with the energy dispersive X-ray spectroscope (SEM-EDS) and Fourier transform-infrared spectroscopy (FTIR). Some characteristic parameters including specific surface area, point of zero point and surface acidity/basicity were also determined. The experimental data was analyzed using various adsorption kinetic models – pseudo-first order, the Ritchie second order, the modified second order, the Elovich and the parabolic diffusion models – to determine the best-fit equation for the adsorption of metal ions onto BC. The results show that the modified Ritchie second order equation and Elovich equation fitted the experimental data well. Also, the parabolic diffusion model fitted the experimental data well at the initial stage of adsorption (

In this study, the NiFe2O4 nanoparticles have been prepared by co-precipitation and calcination process. Using a vibrating sample magnetometer (VSM), transmission electron microscopy (TEM), X-ray diffraction (XRD), energy dispersive spectrometer of X-ray (EDX), and X-ray photoelectron spectroscopy (XPS), the samples obtained by co-precipitation and then by further calcination have been analyzed. The experimental results show that the precursor synthesized by co-precipitation is the composite of both amorphous FeOOH and Ni(OH)2, but has no amorphous NiFe2O4. The results of both EDX and XPS revealed that the FeOOH species is wrapped up by Ni(OH)2 species. In the calcination process, the amorphous composite is dehydrated and transformed gradually into crystalline NiFe2O4 nanoparticles, with the metal ions diffusing. The reaction is different from the one used to prepare other ferrite (e.g., CoFe2O4, MnFe2O4, Fe3O4, etc.) nanoparticles directly by co-precipitation. With increasing calcination temperature, the...

Dietary absorption is a major way for mammals to obtain cholesterol, which is mediated by Niemann-Pick C1-like 1 (NPC1L1) via vesicular endocytosis. One fundamental question in this process is how free cholesterol is efficiently taken up through the internalization of NPC1L1. Using exogenously expressed NPC1L1-EGFP, we show that the lipid raft proteins flotillins associate with NPC1L1 and their localization is regulated by NPC1L1 during intracellular trafficking. Furthermore, flotillins are essential for NPC1L1-mediated cellular cholesterol uptake, biliary cholesterol reabsorption, and the regulation of lipid levels in mice. Together with NPC1L1, they form cholesterol-enriched membrane microdomains, which function as carriers for bulk of cholesterol. The hypocholesterolemic drug ezetimibe disrupts the association between NPC1L1 and flotillins, which blocks the formation of the cholesterol-enriched microdomains. Our findings reveal a functional role of flotillins in NPC1L1-mediated cholesterol uptake and elucidate the formation of NPC1L1–flotillins-postive cholesterol-enriched membrane microdomains as a mechanism for efficient cholesterol absorption.

Weak magnetic ZnFe2O4 nanoparticles were prepared by coprecipitation and treated with different concentrations of Fe(NO3)3 solution. Untreated and treated particles were studied using a vibrating sample magnetometer, transmission electron microscope, by X-ray diffraction, X-ray energy dispersive spectroscopy and X photoelectron spectroscopy. The results showed that, after treatment, the ZnFe2O4/γ-Fe2O3 forms disphase nanoparticles, with enlarged size, enhanced magnetic properties and with a surface parceled with Fe(NO3)3. The size of the particles and their magnetic properties are related to the concentration of the treatment solution. The particle size and magnetic properties could be controlled by controlling the concentration of treating solution, therefore nanoparticles can be more widely used.

Using co-precipitation and processing in FeCl2 solution, Ni–Fe bioxide composite nanoparticles have been prepared. The precursor and the as-prepared nanoparticles are characterized by TEM, XRD, EDX, XPS and VSM. The precursor, consisting of a mixture of FeOOH and Ni(OH)2, has been synthesized using the well-known co-precipitation method. Using heat treatment in FeCl2 solution, at 100 °C, in atmosphere and autoclave, a transition took place in which in addition to the Ni(OH)2 partially dissolving, the precursor was transformed into Ni2O3–Fe2O3 composite nanoparticles of about 10 nm diameter. The transition in FeCl2 solution denoted a transition induced chemical reaction, which is different from the transition induced thermally by calcination technology. In the chemically induced transition, following the dehydration, there is no diffusion of metal ions, as would arise from a thermally induced transition but there is local migration of metal ions to form grains. The chemically induced transition in FeCl2 solution can be part of a new route for the preparation of composite or single phase oxide nanoparticles by the co-precipitation method.

The magnetization curves of MnFe2O4 nanoparticles and self-formed ferrofluids based on these particles have been measured at room temperature. The median size of the particles is 13.67 nm. The specific saturation magnetization is less than the theoretical value for the ferrofluids. In the high field range from 5 kOe to 10 kOe, the higher the particle volume fraction is, the steeper the slope of the magnetization curves is when it approaches saturation. The behavior of the saturation magnetization and the law of approach to saturation are due to the presence of self-assembled aggregates of ring-like micelle structures which form in the absence of the magnetic field and field-induced aggregates, respectively. The field-induced aggregates have a dissipative structure, so that at high field, the law of approach to saturation magnetization is different from the one described using Langevin paramagnetism theory. The large particles in the ferrofluids result in apparent hysteresis.

The Sphingomonas paucimobilis biomass has been successfully utilized to degrade several persistent organic pollutants (POPs). However, few studies have been conducted to use it to remove heavy metals from aqueous solutions. In the present study, biosorption experiments for Cr (VI) were investigated using nonliving biomass of S. paucimobilis isolated from activated sludge, Lianyungang Dapu sewage treatment plant, China. The effects of several parameters including solution pH, contact time, and ionic strength, etc. on Cr (VI) uptake were studied. The biomass was characterized by scanning electron microscopy and energy dispersive X-ray spectroscopy (SEM-EDS) and Fourier transform infrared spectrometer (FTIR). The applicability of the Langmuir and Freundlich models was tested. The correlation coefficients (R) of both models were greater than 0.95. The maximum adsorption capacities were found to be 28.5 mg/g for Cr (VI) at 20°C. The adsorption process was quick and found to follow the pseudo-second-order equat...

With an external magnetic field, a relaxation process is observed when the light transmit through binary ferrofluids composed of ferrimagnetic CoFe2O4 and paramagnetic p-NiFe2O4 nanoparticles similar to ferrofluids consisting only of CoFe2O4. Since only the ferrimagnetic nanoparticles are able to form field-induced chainlike structures for such binary ferrofluids by magnetic interaction between the particles, so the relaxation behavior of the transmitted light is caused mainly by the ferrimagnetic system. In the binary ferrofluids, the paramagnetic nanoparticles, regarded as magnetically polarized gas molecules, are restrained to occupy the space between the ferrimagnetic chains and distribute following the CoFe2O4 particle chains covering and diverging, producing a modulation effect on the relaxation behavior of the transmitted light. The modulation effect can be characterized by range and time parameters that describe the relationship of the relaxation behavior of the transmitted light to the properties of the binary ferrofluids and single CoFe2O4 ferrofluids.

Two new ursane-type triterpene glucosides, 2alpha,3alpha,24-trihydroxyurs-12,18-dien-28-oic acid beta-D-glucopyranosyl ester (1) and 2alpha,3alpha,23-trihydroxyurs-12,19(29)-dien-28-oic acid beta-D-glucopyranosyl ester (2), were isolated from the roots of Rosa laevigata, together with three known compounds: 2alpha,3beta,19alpha-trihydroxyurs-12-en-28-oic acid beta-Dglucopyranosyl ester (3), 2alpha,3alpha,19alpha-trihydroxyurs-12-en-28-oic acid beta-D-glucopyranosyl ester (4) and 2alpha,3beta,19alpha,23-tetrahydroxyurs-12-en-28-oic acid beta-D-glucopyranosyl ester (5). The structures of new compounds were established on the basis of detailed 1D and 2D NMR spectroscopic analyses. Compounds 2 and 5 exhibited modest in vitro antifungal activities against Candida albicans and C. krusei.

An epoxide derived from (-)-shikimic acid was attached to a solid support and used to synthesize over 5000 diverse small molecules. Key transformations include a Lewis acid-catalyzed epoxide opening with amines and an intramolecular Heck reaction with aryl iodides. Compounds derived from this pathway were printed onto small-molecule microarrays and screened for binding to proteins. Compounds that bound to Aurora A kinase were characterized using surface plasmon resonance.

According to the transcriptome dataset of Panax notoginseng, the key geranylgeranyl pyrophosphate synthase gene (GGPPS) in terpenoid backbone biosynthesis was selected to be cloned. Using specific primer pairs combining with RACE (rapid amplification of cDNA ends) technique, the full-length cDNA sequence with 1 203 bp, which containing a 1 035 bp open reading frame, was cloned and named as PnGGPPS. The corresponding full-length DNA sequence contained 2 370 bp, consisted of 1 intron and 2 exons. The deduced protein PnGGPPS contained 344 amino acids and shared more than 73% identity with GGPPS from Ricinus communis and Salvia miltiorrhiza. PnGGPPS also had specific Aspartic acid enrichment regions and other conserved domains, which belonged to the Isoprenoid-Biosyn-C1 superfamily. The quantitative real-time PCR showed that PnGGPPS expressed in different tissues of 1, 2, 3 years old root, stem, leaf and 3 years old flower, and the expression level in 3 years old leaf was significant higher than that in other organs, which suggested that it might not only be involved in the regulation of the growth and development, but also be associated with the biosynthesis of chlorophyll and carotenoids, the development of chloroplast, the shade habit and the quality formation of P. notoginseng.

Two new phenylethanoid glycosides, longissimosides A (I) and B (II), are isolated from the EtOH extract of leaves and stems of Callicarpa longissima together with eight structurally related known compounds.

In this study, two secondary metabolite compounds, trichodimerol and sorbicillin were isolated from the mycelial mass of the marine fungus Trichothecium sp.. It was found that trichodimerol and sorbicillin exhibited strong cytotoxic activity with IC50 values from 6.55 μM to 28.55 μM on three cancer cell lines, HL-60, U937 and T47D. Then HL-60 cells were employed for apoptotic assay. The two compounds could significantly increase the levels of activated caspase-3/7 in a dose-dependent manner and remarkably increase sub-G1 fraction in the cell cycle using flow cytometry, indicating that trichodimerol and sorbicillin potently induced apoptosis in HL-60 cells. Trichodimerol or sorbicillin induced ROS levels also showed dose-dependent increases in HL-60 cells as measured by 2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA), while trichodimerol or sorbicillin induced apoptosis was effectively blocked by the ROS inhibitor N-acetyl-L-cysteine (NAC). Western blot results showed that trichodimerol or sorbicillin could increase phosphorylated p38 levels and decrease ERK and phosphorylated ERK levels in a concentration-dependent manner. Our findings suggest that the pro-apoptosis effects of trichodimerol and sorbicillin were mediated by ROS, while activation of p38 and inhibition of ERK may be involved in these effects.

The application of lipidomics, after genomics, proteomics and metabolomics, offered largely opportunities to illuminate the entire spectrum of lipidome based on a quantitative or semi-quantitative level in a biological system. When combined with advances in proteomics and metabolomics high-throughput platforms, lipidomics provided the opportunity for analyzing the unique roles of specific lipids in complex cellular processes. Abnormal lipid metabolism was demonstrated to be greatly implicated in many human lifestyle-related diseases. In this review, we focused on lipidomic applications in brain injury disease, cancer, metabolic disease, cardiovascular disease, respiratory disease and infectious disease to discover disease biomarkers and illustrate biochemical metabolic pathways. We also discussed the analytical techniques, future perspectives and potential problems of lipidomic applications. The application of lipidomics in disease biomarker discovery provides the opportunity for gaining novel insights into biochemical mechanism.

In a low emulsifier system, the MMA-BA-MAA copolymer emulsions were prepared as seed latices and the seeded emulsion polymerization of MMA-MAA-DVB was consequently carried out to prepare carboxylated core particles. The hydrophobic shell was then synthesized onto the core using styrene, acrylonitrile, and divinylbenzene as comonomers. The hollow latex particles were obtained by alkalization treatment of the core-shell latex particles. The effects of the feeding rate of monomer mixture, contents of emulsifier SDBS and crosslinking agent DVB, and ratio of the monomers during the core stage and shell stage on the morphology and volume expansion of the latex particles were investigated. The results show that the monodispersed hollow latex particles with large size can be obtained when the feeding rate is 0.1 g/min, SDBS content is 0.15 and 0.2 wt % during the core stage and shell stage, respectively, DVB contents are 1% during the preparation of shell copolymers, and the monomer ratio of the core particle to shell layer is 1: 8.

C23H24N4O9S, triclinic, P1̄ (no. 2), a = 8.297(1) Å, b = 11.199(1) Å, c = 13.876(2) Å, α = 88.856(2)°, β = 76.540(2)°, γ = 80.098(2)°, V = 1235.0 Å3, Z = 2, Rgt(F) = 0.0405, wRref(F2) = 0.1084, T = 296 K.

We have developed a high-throughput yeast two-hybrid screening system (HTP-YTH) that incorporates yeast gap-repair cloning, multiple positive ( ADE2, HIS3, lacZ) and negative ( URA3-based) selection schemes to reduce the incidence of negative and false positive clones, and automation of laboratory procedures to increase throughput. This HTP-YTH system has been applied to the study of protein-protein interactions that are involved in rice defense signal transduction pathways. More than 100 genes involved in plant defense responses were selected from DuPont's rice expressed sequence tag (EST) databases as baits for HTP-YTH screening. Results from YTH screening of eight of these rice genes are presented in this paper. Not only have we identified known protein-protein interactions, but we have also discovered novel interactions, which may ultimately reveal the regulatory network of host defense signal transduction pathways. We have demonstrated that our HTP-YTH method can be used to map protein-protein interaction networks and signal transduction pathways in any system. In combination with other approaches, such efficient YTH screens can help us systemically to study the functions of known and unknown genes in the genomics era.

The present study investigated the effects of saikosaponin a (SSA), a natural bioactive compound from Radix bupleuri, on memory deficit and neuroinflammation in the Aβ-treated C57BL/6 mouse model of AD. Different doses of SSA (4, 8, or 16 mg/kg) were intragastrically administered once a day after intracerebroventricular Aβ injection (2.5 μg/μl, 4 μl). 15 days after Aβ injection, behavioural experiments including the Morris water maze and novel object recognition (NOR) tests were performed. 21 days after the Aβ injection, the concentrations of ROS, IL-1β and TNF-α, as well as expression of NADPH oxidase subunits in hippocampal tissue were all assessed. Results from the Morris water maze test revealed that Aβ injection could remarkably increase the escape latency time while SSA could significantly decrease it. In the NOR test, SSA significantly increased the Preference Index (PI), which remarkably decreased in the Aβ injection group. ROS, IL-1β and TNF-α levels all increased after Aβ injection, while SSA significantly reduced the concentration of ROS, IL-1β and TNF-α at doses of 8 mg/kg and 16 mg/kg. Protein expression of NADPH oxidase subunits gp91phox and p47phox were significantly reduced by SSA at doses of 8 and 16 mg/kg. The results indicated that SSA could ameliorate learning and memory impairment induced by Aβ treatment and these effects were mediated through inhibition of pro-inflammatory mediators in hippocampal tissue. Furthermore, NADPH oxidase may be involved in this effect. SSA may be a potent therapeutic drug for the treatment of neurodegenerative diseases associated with neuroinflammation and cognitive impairment such as AD.

To reflect the extent of thermolesion of ganglion by testing the change of trigeminal somatosensory-evoked potential (TSEP) before and after ganglion radiofrequency thermocoagulation surgery (GRT), and evaluate long-term clinic effect by follow-up visiting of 1 year.Patients with idiopathic

Hybrid photoelectric joint transform correlator can realize automatic real-time recognition with high precision through the combination of optical devices and electronic devices. When recognizing targets with low contrast using photoelectric joint transform correlator, because of the difference of attitude, brightness and grayscale between target and template, only four to five frames of dynamic targets can be recognized without any processing. CCD camera is used to capture the dynamic target images and the capturing speed of CCD is 25 frames per second. Automatic threshold has many advantages like fast processing speed, effectively shielding noise interference, enhancing diffraction energy of useful information and better reserving outline of target and template, so this method plays a very important role in target recognition with optical correlation method. However, the automatic obtained threshold by program can not achieve the best recognition results for dynamic targets. The reason is that outline information is broken to some extent. Optimal threshold is obtained by manual intervention in most cases. Aiming at the characteristics of dynamic targets, the processing program of improved automatic threshold is finished by multiplying OTSU threshold of target and template by scale coefficient of the processed image, and combining with mathematical morphology. The optimal threshold can be achieved automatically by improved automatic threshold processing for dynamic low contrast target images. The recognition rate of dynamic targets is improved through decreased background noise effect and increased correlation information. A series of dynamic tank images with the speed about 70 km/h are adapted as target images. The 1st frame of this series of tanks can correlate only with the 3rd frame without any processing. Through OTSU threshold, the 80th frame can be recognized. By automatic threshold processing of the joint images, this number can be increased to 89 frames. Experimental results show that the improved automatic threshold processing has special application value for the recognition of dynamic target with low contrast.

The recent successes in the immunotherapy field demonstrate that a successful immune response to cancer can lead to meaningful anti-tumor responses. Antibodies blocking immune checkpoint molecules (e.g. CTLA4, and PD-1) lead to effective T cell responses especially in tumors that are immune active. Immune cells that have not received as much attention in cancer immunotherapy are neutrophils. Neutrophils are the most abundant white blood cells, which protect us from microbial infections. They are the first line of innate defense: they arrive early on in the course of infection, phagocytose, and release the content of their granules, including reactive oxygen species and enzymes. Neutrophils also communicate with other immune cells, such as macrophages, dendritic cells, and T cells, by releasing cytokines and chemokines. There has long been a call to recruit these professional killers to fight cancer. However, an approach that mediates the recruitment of neutrophils has to lead to acute as opposed to chronic inflammation to overcome the suppressive environment that tumors surround themselves with. We have developed a novel immunotherapy platform technology, termed mAbXcite, which directs and activates neutrophils to kill cancer cells in a targeted manner. The targeting is achieved by using monoclonal antibodies that are chemically linked to beta-1,6-glucan, a saccharide found on the cell walls of fungal species which recruits and activates neutrophils. The resulting mAb construct attracts mediators of acute inflammation, notably neutrophils, leading to rapid destruction of cancer cells. We will present proof-of concept of the mAbXcite technology using two validated antibodies, the anti-Her2 Trastuzumab and the anti-EGFR Cetuximab. In preclinical xenograft models, these mAbXcite constructs demonstrate significantly greater efficacy than the original antibody in resistant tumor models. Furthermore, some mice show complete regressions and do not grow tumors upon rechallenge, suggesting a long term immune response. We will also provide pharmacodynamics results, primarily neutrophil infiltration by histology and live imaging, as well as pharmacokinetics results, demonstrating that the linked oligosaccharide is stable and is not affecting the half-life of the antibodies. This novel immunotherapy platform is suitable for peripheral membrane targets that are overexpressed on tumors, and does not require antibody internalization. The unique mechanism of action of recruiting and activating neutrophils against cancer could be synergistic with other immunotherapeutic approaches.

Hyperlipidemia is an important public health problem with increased incidence and prevalence worldwide. Current clinical biomarkers, triglyceride, total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol lack the necessary specificity and sensitivity and only increase significantly after serious dyslipidemia. Therefore, sensitive biomarkers are needed for hyperlipidemia. Hyperlipidemia-specific biomarkers would improve clinical diagnosis and therapeutic treatment at early disease stages. The aim of metabolomics is to identify untargeted and global small-molecule metabolite profiles from cells, biofluids, and tissues. This method offers the potential for a holistic approach to improve disease diagnoses and our understanding of underlying pathologic mechanisms. This review summarizes analytical techniques, data collection and analysis for metabolomics, and metabolomics in hyperlipidemia animal models and clinical studies. Mechanisms of hypolipemia and antilipemic drug therapy are also discussed. Metabolomics provides a new opportunity to gain insight into metabolic profiling and pathophysiologic mechanisms of hyperlipidemia.

Microglia‑mediated neuroinflammation is key in the pathogenesis of Alzheimer's disease (AD). Several studies have suggested that NADPH oxidase contributes to microglia‑mediated neuroinflammation. Resveratrol, which is a natural polyphenolic compound, exerts neuroprotective effects in AD due to its anti‑inflammatory properties. The present study aimed to investigate the effects of resveratrol on the activation of oligomeric amyloid β (oAβ)‑induced BV‑2 microglia, and to determine the role of NADPH oxidase in these effects. Microglial proliferation was measured by high‑content screening cell counting and using a bromodeoxyuridine incorporation assay. In addition, the levels of reactive oxygen species (ROS), nitric oxide (NO), tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β were assessed. The results of the present study demonstrated that resveratrol inhibited the proliferation of oAβ‑induced microglia and the production of pro‑inflammatory factors, including ROS, NO, TNF‑α and IL‑1β. Subsequent mechanistic investigations demonstrated that resveratrol inhibited the oAβ‑induced mRNA and protein expression levels of p47phox and gp91phox. These results suggested that NADPH oxidase may be a potential target for AD treatment, and resveratrol may be a valuable natural product possessing therapeutic potential against AD.

To classify colorectal carcinoma (CRC) by TNM staging integrated with the gene expression profile and copy number variation (CNV).Profile data of gene expression and CNV of CRC were downloaded from public database and processed with batch bias adjustment, quartile normalization, missing value estimation and feature filtration. The processed profiles of mRNA and CNV were introduced into the codes of Bayesian consensus clustering (BCC) method and were used to calculate the subclasses of CRC. With the follow-up information of disease free survival of CRC patients, the prognostic values of the subclasses was investigated and the software of function enrichment analysis was employed to discover the major pathway signaling to each interesting subclass. All statistic analyses were performed under R-3.0.1 environment or by using SPSS 16.0 software.Profile data of gene expression and corresponding CNV from 335 CRC patients with TNM stage Ⅱ-Ⅲ and followed-up information were obtained. After feature filtering, the profiles contained 1578 probes of mRNA and 345 location of CNV. Four CRC subclasses were identified by the integrative analysis with BCC, and the concordances of BCC subclasses and each of gene-based subclasses (Cramer's V=0.49), CNV-based subclasses (Cramer's V=0.51) and Marisa's subclasses (Cramer's V=0.32) were statistically significant (Ps0.001). Among BCC subclasses, BCC-I had a favorable prognosis, while BCC-Ⅳ had more unfavorable prognosis. The differences of prognosis were significant among BCC-I, BCC-(Ⅱ+Ⅲ) and BCC-Ⅳ with an overall log-rank P0.001. The top enriched function was DNA damage and repair signaling when BCC-I compared to BCC-Ⅳ, and the new subgroups classified by the genes associated with enriched signaling had the better prognostic value than BCC subclasses but both of them were significantly correlated (Cramer's V=0.39, P0.001).BCC method is effective to integrate multi-type genomic data for molecular classification of colorectal carcinoma, and the BCC-Ⅳ subclass has poor prognosis, which may be associated with the decreased repairing function of DNA damage.

Somatic cell genetics is a powerful approach for unraveling the regulatory mechanism of cholesterol metabolism. However, it is difficult to identify the mutant gene(s) due to cells are usually mutagenized chemically or physically. To identify important genes controlling cholesterol biosynthesis, an unbiased forward genetics approach named validation-based insertional mutagenesis (VBIM) system was used to isolate and characterize the 25-hydroxycholesterol (25-HC)-resistant and SR-12813-resisitant mutants. Here we report that five mutant cell lines were isolated. Among which, four sterol-resistant mutants either contain a truncated NH2-terminal domain of sterol regulatory element-binding protein (SREBP)-2 terminating at amino acids (aa) 400, or harbor an overexpressed SREBP cleavage-activating protein (SCAP). Besides, one SR-12813 resistant mutant was identified to contain a truncated COOH-terminal catalytic domain of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase). This study demonstrates that the VBIM system can be a powerful tool to screen novel regulatory genes in cholesterol biosynthesis.

License plate recognition (LPR) is an important part of the vehicle detection system, which plays a significant role in traffic management and has a variety of applications. This paper presents a license plate recognition method based on pulse coupled neural network (PCNN) and template matching. One PCNN is implemented to segment the gray image containing the License plate, and another PCNN is applied to extract the characters from the located plate. The method has some advantages when the image is polluted or photographed without enough illumination. After the plate characters are extracted, they are compared with the pre-defined template using template matching. Based on the similarity between the extracted characters and template characters, the license is recognized. Simulations have been presented for illustrative purposes.