201. Inhibitory Effects of Melanin Secretion on B16 Melanoma cell of Cordyceps militaris Water Extract
- Author
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Wool-Soon Jo, Eun Young Kang, Min-Ho Jeong, Jae-Dong Lee, Jae-Yun Lee, Yoo Jin Choi, and Byung-Hyouk Nam
- Subjects
chemistry.chemical_classification ,Ecology ,Melanoma ,Tyrosinase ,Cell ,Plant Science ,Biology ,medicine.disease ,Inhibitory postsynaptic potential ,biology.organism_classification ,medicine.anatomical_structure ,Enzyme ,chemistry ,Biochemistry ,Cordyceps militaris ,medicine ,MTT assay ,Tyrosine ,Ecology, Evolution, Behavior and Systematics - Abstract
The present study aims to evaluate Cordyceps militaris water extract (CMWE) with a view to develop melanogenesis inhibitors. Inhibitory activities of CMWE against tyrosinase, L-DOPA(L-3,4-dihydroxyphenylalanine) oxidation, and melanin biosynthesis in B16 mouse melanoma cells were investigated. CMWE, at 5000 μg/ml, inhibited tyrosinase activity of 71% and DOPA oxidation of 40% as reacting with L-DOPA. Furthermore, B16 mouse melanoma cell survived over 50% from low to high dose on MTT assay, and CMWE markedly inhibited (>50%) melanin synthesis at 5000 μg/ml. The inhibitory effect of CMWE on melanogenesis was attributed to enhancement of tyrosinase degradation. Key enzyme of melanin biosynthesis is tyrosinase which catalyses a beginning step from tyrosine to DOPA quinine and melanin formation step, respectively. These results indicated that CMWE may be a potential source of novel whitening agents for cosmetic or therapeutic application.
- Published
- 2010