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Copper oxide nanoparticles (CuO-NPs) have piqued the interest of agricultural researchers due to their potential application as fungicides, insecticides, and fertilizers. The Serratia sp. ZTB29 strain, which has the NCBI accession number MK773873, was a novel isolate used in this investigation that produced CuO-NPs. This strain can survive concentrations of copper as high as 22.5 mM and can also remove copper by synthesizing pure CuO-NPs. UV-VIS spectroscopy, DLS, Zeta potential, FTIR, TEM, and XRD techniques were used to investigate the pure form of CuO-NPs. The synthesized CuO-NPs were crystalline in nature (average size of 22 nm) with a monoclinic phase according to the XRD pattern. CuO-NPs were found to be polydisperse, spherical, and agglomeration-free. According to TEM and DLS inspection, they ranged in size from 20 to 40 nm, with a typical particle size of 28 nm. CuO-NPs were extremely stable, as demonstrated by their zeta potential of −15.4 mV. The ester (C=O), carboxyl (C=O), amine (NH), thiol (S-H), hydroxyl (OH), alkyne (C-H), and aromatic amine (C-N) groups from bacterial secretion were primarily responsible for reduction and stabilization of CuO-NPs revealed in an FTIR analysis. CuO-NPs at concentrations of 50 μg mL−1 and 200 μg mL−1 displayed antibacterial and antifungal activity against the plant pathogenic bacteria Xanthomonas sp. and pathogenic fungus Alternaria sp., respectively. The results of this investigation support the claims that CuO-NPs can be used as an efficient antimicrobial agent and nano-fertilizer, since, compared to the control and higher concentrations of CuO-NPs (100 mg L−1) considerably improved the growth characteristics of maize plants.

Fungal communities in agricultural soils are assumed to be affected by climate, weather, and anthropogenic activities, and magnitude of their effect depends on the agricultural activities. Therefore, a study was conducted to investigate the impact of the portfolio of management practices on fungal communities and soil physical–chemical properties. The study comprised different climate-smart agriculture (CSA)-based management scenarios (Sc) established on the principles of conservation agriculture (CA), namely, ScI is conventional tillage-based rice–wheat rotation, ScII is partial CA-based rice–wheat–mungbean, ScIII is partial CSA-based rice–wheat–mungbean, ScIV is partial CSA-based maize–wheat–mungbean, and ScV and ScVI are CSA-based scenarios and similar to ScIII and ScIV, respectively, except for fertigation method. All the scenarios were flood irrigated except the ScV and ScVI where water and nitrogen were given through subsurface drip irrigation. Soils of these scenarios were collected from 0 to 15 cm depth and analyzed by Illumina paired-end sequencing of Internal Transcribed Spacer regions (ITS1 and ITS2) for the study of fungal community composition. Analysis of 5 million processed sequences showed a higher Shannon diversity index of 1.47 times and a Simpson index of 1.12 times in maize-based CSA scenarios (ScIV and ScVI) compared with rice-based CSA scenarios (ScIII and ScV). Seven phyla were present in all the scenarios, where Ascomycota was the most abundant phyla and it was followed by Basidiomycota and Zygomycota. Ascomycota was found more abundant in rice-based CSA scenarios as compared to maize-based CSA scenarios. Soil organic carbon and nitrogen were found to be 1.62 and 1.25 times higher in CSA scenarios compared with other scenarios. Bulk density was found highest in farmers' practice (Sc1); however, mean weight diameter and water-stable aggregates were found lowest in ScI. Soil physical, chemical, and biological properties were found better under CSA-based practices, which also increased the wheat grain yield by 12.5% and system yield by 18.8%. These results indicate that bundling/layering of smart agricultural practices over farmers' practices has tremendous effects on soil properties, and hence play an important role in sustaining soil quality/health.

Drip irrigation and mulching are often used to alleviate the problem of poor water management in many crops; however, these technologies have not yet been tested for applying water at critical stages of guava orchard growth in subtropical humid Tarai regions of India to improve the yield and quality. A field experiment was conducted over 2020 and 2021 which included three irrigation strategies: severe deficit irrigation (DI50), moderate deficit irrigation (DI75), and full irrigation (FI100), as well as four mulching methods: silver-black mulch (MSB), black mulch (MB), organic mulch (MOM), and a control without mulch (MWM). The results showed that both the relative leaf water content (RLWC) and the proline content exhibited an increasing trend with a decrease in the irrigation regime, resulting in a 123% increase in the proline content under DI50 conditions compared with FI100, while greater plant growth was recorded in fully irrigated plants and using silver-black mulch. Leaf nutrient analysis showed that FI100 and MOM produced significantly higher concentrations of all nutrients. However, moderate deficit irrigation (DI75) along with silver-black mulch (MSB) produced higher numbers of fruits per plant, higher average fruit weights, higher fruit yields, and maximum ascorbic acid contents. The irrigation water productivity (IWP) decreased with an increase in the irrigation regime; from severe water deficit to full irrigation, resulting in a 33.79% improvement in IWP under DI50 conditions as compared with FI100. Regression analysis outperforms principal component regression analysis for fruit yield prediction, with adjusted R2 = 89.80%, RMSE = 1.91, MAE = 1.52, and MAPE = 3.83. The most important traits affecting the fruit yield of guava, based on stepwise regression, were leaf proline, leaf Cu, fruit weight, and IWP.

Abstract There is an urgent need to develop disease-modifying therapies to treat neurodegenerative diseases which pose increasing challenges to global healthcare systems. Prion diseases, although rare, provide a paradigm to study neurodegenerative dementias as similar disease mechanisms involving propagation and spread of multichain assemblies of misfolded protein (“prion-like” mechanisms) are increasingly recognised in the commoner conditions such as Alzheimer’s disease. However, studies of prion disease pathogenesis in mouse models showed that prion propagation and neurotoxicity can be mechanistically uncoupled and in vitro assays confirmed that highly purified prions are indeed not directly neurotoxic. To aid development of prion disease therapeutics we have therefore developed a cell-based assay for the specific neurotoxicity seen in prion diseases rather than to simply assess inhibition of prion propagation. We applied this assay to examine an anti-prion protein mouse monoclonal antibody (ICSM18) known to potently cure prion-infected cells and to delay onset of prion disease in prion-infected mice. We demonstrate that whilst ICSM18 itself lacks inherent neurotoxicity in this assay, it potently blocks prion disease-associated neurotoxicity.

Abstract Background The role of serum Procalcitonin (PCT) in adults in diagnosis of Community acquired pneumonia (CAP) is well established, however, role in pediatric CAP remains controversial. Objectives The objective of this study was to investigate the utility of serum procalcitonin in differentiating bacterial community-acquired lower respiratory tract infection from non-bacterial respiratory infection in children; radiologically confirmed pneumonia was used as the reference. In addition, we assessed the utility of adding the PCT assay to the clinical criteria for diagnosis of pneumonia. Study design Subanalysis of a larger prospective,multicentriccohort study. Participants Children, 2 months to 59 months of age, attending paediatric OPD of 5 urban tertiary care hospitals, suffering from acute respiratory infection (ARI). Intervention Detailed clinical history and examination findings of enrolled children were recorded on predesigned case record form. Samples for PCT were obtained at admission and were measured centrally at the end of the study except for one site using VIDAS® B.R.A.H.M.S PCT kit (Biomerieux SA, France). Outcomes Sensitivity and specificity of procalcitonin for diagnosis of radiologically confirmed pneumonia. Results Serum Procalcitonin was measured in 370 patients; median (IQR) age of these children being 12 (7, 22) months, 235 (63.5%) were boys. The median (IQR) serum procalcitonin concentration was 0.1(0.05, 0.4) ng/mL.Sensitivity and specificity of raised PCT (> 0.5 ng/mL) for pneumonia as per any CXR abnormalities were 29.7% and87.5%,(P 0.5 ng/mL) is significantly associated with radiographic pneumonia but not with pneumonia based on WHO criteria.However, it can act as a surrogate marker for severe pneumonia.

Abstract Background Acute respiratory infections (ARI) are the leading cause of morbidity and mortality in children below 5 years of age. Methods This multisite prospective observational study was carried out in the Pediatrics’ out-patient departments of 5 medical colleges across India with an objective to assess the feasibility of establishing Acute Respiratory Infection Treatment Unit (ATU) in urban medical college hospitals. ATU (staffed with a nurse and a medical officer) was established in the out-patient areas at study sites. Children, aged 2–59 months, with cough and/ breathing difficulty for

Abstract In water scarce regions of South Asia, diversification of rice with maize is being advocated towards sustainability of cereal-based cropping systems. Adoption of innovative agronomic management practices, i.e., conservation agriculture (CA) and sub-surface drip irrigation (SSDI) are considered as key strategies for much needed interventions to address the challenges of water scarcity under projected climate change. Benefits from CA and SSDI concerning water economy are well-established, however, information about their complementarity and water budgeting in cereal-based systems are lacking. A field study was conducted with process-based model (HYDRUS-2D) to understand water transport, root water uptake and components of soil water balance in maize grown in rotation with wheat after five years of continuous adoption of conservation agriculture. In this study, altogether eight treatments comprising of 6 CA+ treatments (CA coupled with SSDI); permanent beds using sub-surface drip (PB-SSD) with (WR) and without (WOR) crop residue at different N rates, 0, 120 and 150 kg N ha−1 were compared with CA (PB using furrow irrigation-FI with crop residue-120 kg N ha−1) and conventional tillage practices (CT) (CT using FI without crop residue-120 kg N ha−1). Results showed that the model could simulate the daily changes in profile soil water content with reasonable accuracy in all the treatments. Simulated soil water balance indicated higher cumulative root water uptake (CRWU), lower cumulative evaporation (CE) and higher soil water retention in CA+ (PB-SSD+ crop residue at 150 and 120 kg N ha−1) than CA and CT plots. Hydrus-2D model efficiency > 0, RMSE between 0.009–0.026 and R2 value between 0.80–0.92 at P

Abstract Climate-smart agriculture (CSA)-based management practices are getting popular across South-Asia as an alternative to the conventional system for particular weed suppression, resources conservation and environmental quality. An 8-year study (2012–2013 to 2019–2020) was conducted to understand the shift in weed density and diversity under different CSA-based management practices called scenarios (Sc). These Sc involved: Sc1, conventional tillage (CT)-based rice–wheat system with flood irrigation (farmers’ practice); Sc2, CT-rice, zero tillage (ZT)-wheat–mungbean with flood irrigation (partial CA-based); Sc3, ZT rice–wheat–mungbean with flood irrigation (partial CSA-based rice); Sc4, ZT maize–wheat–mungbean with flood irrigation (partial CSA-based maize); Sc5, ZT rice–wheat–mungbean with subsurface drip irrigation (full CSA-based rice); and Sc6, ZT maize–wheat–mungbean with subsurface drip irrigation (full CSA-based maize). The most abundant weed species were P. minor > A. arvensis > M. indicus > C. album and were favored by farmers’ practice. However, CSA-based management practices suppressed these species and favored S. nigrum and R. dentatus and the effect of CSAPs was more evident in the long-term. Maximum total weed density was observed for Sc1, while minimum value was recorded under full CSA-based maize systems, where seven weed-species vanished, and P. minor density declined to 0.33 instead of 25.93 plant m−2 after 8-years of continuous cultivation. Full CSA-based maize–wheat system could be a promising alternative for the conveniently managed rice–wheat system in weed suppression in north-west India.

For the characterization of fiber-reinforced soil under static loading conditions, there is a wealth of literature. However, study on the dynamic behavior of fiber-reinforced soil is very limited. Now that earthquakes are occurring frequently around the world, the dynamic soil analysis has become important for all classes of geotechnical engineering problems. Through numerical simulation of triaxial specimens, the current study explores the behaviour of fiber-reinforced cohesionless soil. The numerical model was validated using existing laboratory triaxial compression testing literature. The stress–strain response of fiber-reinforced sand has been investigated using static and cyclic triaxial testing, as well as other combinations of fiber contents. Fiber-reinforced sand is tested for bulk modulus, shear modulus, and damping values. Effects of fiber contents on static and dynamic stress–strain response of fiber-reinforced soil are highlighted. It has been observed from results that, with an increase in fiber content modulus of elasticity, bulk modulus and shear modulus values increase while damping coefficients decrease for the same. It is believed that the highlighted numerical approach will be an alternative to laboratory experiments to determine the dynamic properties of fiber-reinforced soil. [ABSTRACT FROM AUTHOR]

Chest X-ray, the established standard of confirming endotracheal tube (ETT) position, has important drawbacks including radiation exposure. Point-of-care airway ultrasound, which has been insufficiently studied in children, can overcome these problems. This was a prospective cross-sectional study done on children aged 2 months to 17 years undergoing intubation with cuffed ETT in the PICU. The ETT cuff was filled with saline and three ultrasonographic techniques were used– 1) Suprasternal (SS) method 2) Cricoid (CC) metho and 3) Tracheal ring (TR) method. Position of the ETT as determined by ultrasound and X-ray were compared. The main outcomes were sensitivity, specificity, and area under curve (AUC) for ultrasound-based methods vs. X-ray. For the TR method, concordance between the X-ray and ultrasound categories were taken. Total 62 patients were enrolled. The sensitivity and specificity of SS method were 71% (95% CI: 57–83%) and 100% (40–100%). The CC distance method had an AUC of 0.94 (95% CI: 0.86, 1.0). In the TR method, 98% of correct position on X-ray were correctly classified by USG. The agreement between X-ray and ultrasound categories with the cuff between the first and third tracheal rings, was very good [kappa (95% CI): 0.87 (0.70, 1.00), p ≤0.001)]. Bedside ultrasound is a good method to confirm ETT depth in children. The tracheal ring method had the best diagnostic accuracy and is easy to perform. The new method using cricoid cuff distance needs further validation in different ICU settings. • Chest X-ray the current gold standard for confirming correct endotracheal tube (ETT) position is unsafe. • Ultrasonography in comparison is safer, faster to obtain and easily accessible in ICUs. • We conducted this prospective cross-sectional study in 62 patients. • Three techniques of USG: suprasternal, cricoid and tracheal ring methods were studied. • We found USG to reliably confirm ET tube depth at the bedside. • Of the three methods, the tracheal ring method had the best diagnostic accuracy. [ABSTRACT FROM AUTHOR]

Bitter gourd (Momordica charantia L.) is an important vine crop of the Cucurbitaceae family and is well known for its high nutritional and medicinal values. However, the genetic variation remains largely unknown. Herein, 96 diverse bitter gourd genotypes were undertaken for diversity analysis using 10 quantitative traits, and 82 simple sequence repeat (SSR) markers. Out of 82 SSRs, 33 were polymorphic and the mean polymorphism information content (PIC) value was 0.38. Marker, JY-003 revealed a maximum (0.81) PIC value and, the number of alleles per locus ranged from 2 to 7 (average 3.46). The value of gene diversity showed the presence of a significant level of polymorphism among these genotypes. The unweighted pair group method (UPGMA) cluster analysis grouped the genotypes into two major clusters of which Cluster I comprised mostly small and medium-fruited genotypes of both M. charantia var. charantia and M. charantia var. muricata, whereas Cluster II included mostly long and extra-long fruited genotypes. Furthermore, these genotypes were divided into six distinct groups based on population structure analysis. The diversity analysis based on 10 quantitative traits revealed that earliness and high-yielding ability were exhibited by the predominantly gynoecious line DBGS-21-06 followed by DBGS-48-00. The principal component analysis (PCA) revealed that the first two components exhibited more than 50% of the total genetic variation. The present study deciphered a higher magnitude of agro-morphological and genetic diversity in 96 bitter gourd genotypes. Therefore, trait-specific genotypes identified in this study could be utilized in breeding programmes directed towards the development of improved cultivars and hybrids of bitter gourd.

A field experiment was conducted at the Instructional farm, Rajasthan College of Agriculture, Udaipur for two years (2017–18 and 2018–19) during the winter (rabi) season. The experiments were arranged in a randomized block design (RBD) manner with 3 replications. The results showed that, seed inoculation with biofertilizers (Azotobacter + Phosphorus solubilizing bacteria + Potash mobilizing bacteria + Zinc solubilizing bacteria) improved physico-chemical properties of soil except bulk density, particle density, pH, EC and showed higher availability of nutrients over control plot. Whereas in case of vermicompost (VC), physical properties such as BD, PD, porosity, WHC and chemical properties like pH, EC, CEC, OC and available nutrients were found distinctly enhanced under 50% VC at sowing + 50% VC at tillering in post-harvest soil over control. However, 50% VC at sowing + 50% VC at tillering significantly reduced bulk density, particle density, pH and EC. Furthermore, both biofertilizers and split application of vermicompost also significantly improved the productivity of wheat. It is concluded that application of biofertilizers and vermicompost could reliably be used to improve soil physico-chemical properties of wheat cultivated soils.

South Asia is a global hotspot for climate change with enormous pressure on land and water resources for feeding the burgeoning population. The agricultural production systems are highly vulnerable in the region and is primarily dominated by small and marginal farmers with intensive farming practices that had favored the loss of carbon (C) from soil. This review discusses the potential of soil and crop management practices such as minimum/reduced/no-tillage, use of organic manure, balanced and integrated plant nutrient application, precision land levelling, precision water and pest management, residue management, and cropping system optimization to maintain the C-equilibrium between soil and atmosphere and to enhance the C-sequestration in the long run. Results of meta-analysis show a potential 36% increase in soil organic C stock in the top 0–15 cm layer in this region which amounts to ∼18 Mg C stocks ha−1. Improved management practices across crops and environment may reduce methane em0ission by 12% resulting in an 8% reduction in global warming potential (GWP), while non-submerged condition led to a 51% GWP reduction in rice. Conservation agriculture and precision fertilization also reduced GWP by 11 and 14%, respectively. Although several innovative climate resilient technologies having significant potential for C-sequestration have been developed, there is an urgent need for their scaling and accelerated adoption to increase soil C-sequestration. Policies and programs need to be devised for incentivizing farmers to adopt more C-neutral or C-positive agricultural practices. The national governments and other agencies should work towards C farming together with global initiatives such as the “4 per 1000” Initiative and Global Soil Partnership, and regional public-private partnership initiatives on carbon credits for Regenerative Agriculture such as by Grow Indigo-CIMMYT-ICAR in India, in addition to research and policy changes. This will be vital for the success of soil C sequestration towards climate action in South Asia.

OBJECTIVE: To determine the frequency of cognitive deficits in patients of Depressive disorder. METHODOLOGY: This cross-sectional study was conducted at Jinnah Post Graduate Medical Centre (JPMC), Karachi,from September 2018 to March 2019. The sample size of 250 was calculated through customary techniques, and the sampling technique was non-probability consecutive sampling. Those patients who were diagnosed with cases of depressive disorder were enrolled in the study. The data were analyzed using SPSS (Statistical Packages of Social Sciences) version 22.0. RESULTS: Out of the total of 250 cases, 114 (45.60%) were males, and 136 (54.40%) were females with an average age of 33.62±11.07 years. Among 250, the majority, 178 (71.20%), were married and Illiterate 90 (36.00%). Among all participants, 136 (54.4%) belonged to middle socio-economic and 120 (68.0%) were household by occupation. Out of 250 cases, 135 (54%) were drug naïve, while 114 (45.6%) were on active treatment. Cognitive dysfunction was present among 169 (67.6%). Educational status, treatment status, and duration of diseases were considerably related, with cognitive dysfunction having a p-value of less than 0.05. CONCLUSION: The rate of Cognitive dysfunctions among patients with depressive disorder is high and alarming.

Abstract Micronutrient malnutrition or hidden hunger remains a major global challenge for human health and wellness. The problem results from soil micro- and macro-nutrient deficiencies combined with imbalanced fertilizer use. Micronutrient-embedded NPK (MNENPK) complex fertilizers have been developed to overcome the macro- and micro-element deficiencies to enhance the yield and nutritive value of key crop products. We investigated the effect of foliar applications of an MNENPK fertilizer containing N, P, K, Fe, Zn and B in combination with traditional basal NPK fertilizers in terms of eggplant yield, fruit nutritive quality and on soil biological properties. Applying a multi-element foliar fertilizer improved the nutritional quality of eggplant fruit, with a significant increases in the concentration of Fe (+ 26%), Zn (+ 34%), K (+ 6%), Cu (+ 24%), and Mn (+ 27%), all of which are essential for human health. Increasing supply of essential micronutrients during the plant reproductive stages increased fruit yield, as a result of improved yield parameters. The positive effect of foliar fertilizing with MNENPK on soil biological parameters (soil microbial biomass carbon, dehydrogenase, alkaline phosphatase) also demonstrated its capacity to enhance soil fertility. This study suggests that foliar fertilizing with a multi-nutrient product such as MNENPK at eggplant flowering and fruiting stages, combined with the recommended-doses of NPK fertilizers is the optimal strategy to improve the nutritional quality of eggplant fruits and increase crop yields, both of which will contribute to reduce micronutrient malnutrition and hunger globally.

Introduction: Dyslipidemia-hypertension proves to be a major risk factor for cardiovascular diseases. In order to achieve better adherence and cost-effectiveness than free equivalent combination therapies, a fixed-dose combination therapy with telmisartan (TEL), rosuvastatin calcium (ROS) and amlodipine besylate (AML) is required in this type of patients. Aim: A simple, selective and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated for estimation of telmisartan, rosuvastatin calcium, and amlodipine besylate in synthetic mixture. Materials and methods: Chromatographic separation was performed on a reversed-phase Luna C18 100Å column (250 mm × 4.6 mm i.d., particle size 5 μ) using an isocratic elution of mobile phase consisting of methanol and acetonitrile (pH 3.5 adjusted by ortho-phosphoric acid) (60:40 v/v) at a flow rate of 1.0 ml/min. Results: Ultraviolet (UV) detection was performed at 242 nm and retention time of telmisartan, rosuvastatin calcium and amlodipine besylate was found to be 2.67, 4.70, and 7.44 min, respectively. The calibration curve was linear (correlation coefficient >0.999) in the selected range of analyte. Conclusions: The method was validated for accuracy, precision, linearity, limit of detection, limit of quantitation and ruggedness. The system suitability parameter, such as theoretical plate, asymmetry, and resolution between standard five replicate were well within the limits.

Agriculture in Indo-Gangetic plains (IGP) of India is facing challenges of food security, low factor productivity, lowest per capita landholding and natural resource sustainability in the changing climate. Prevalent rice-wheat system (RWS) in this region requires more labour, water and chemical inputs leading to excessive groundwater exploitation and soil degradation. Present enterprise mix diversification study was carried out to improve productivity, profitability and resource use efficiency of the smallholders in reclaimed sodic lands of IGP during 2007–2015 at ICAR-Central Soil Salinity Research Institute, Karnal, India. Total 2.0 ha area was diversified under grain, vegetable, horticulture, fodder, fisheries, livestock and poultry production systems. This system produced ∼20% higher (13.3 tha−1) rice equivalent yield (REY) than RWS(11.1 tha−1). This system also generated 15.3% higher eight years average net income ($ 5363), than RWS ($ 4650/-), and 875 man-days/annum employment. By recycling this system used ∼50% lesser water than RWS and also improved soil health. On farm production and availability of cereals, vegetables, fruits, milk, eggs, fish, etc. in the system also to ensure food and nutritional security of the farm family. Overall the enterprise mix diversification system enhanced agricultural sustainability with higher productivity, income, employment generation and minimized climatic hazards.

Conventional weed management approaches are inefficient and non-suitable for integration with smart agricultural machinery. Automatic identification and classification of weeds can play a vital role in weed management contributing to better crop yields. Intelligent and smart spot-spraying system's efficiency relies on the accuracy of the computer vision based detectors for autonomous weed control. In the present study, feasibility of deep learning based techniques (Alexnet, GoogLeNet, InceptionV3, Xception) were evaluated in weed identification from RGB images of bell pepper field. The models were trained with different values of epochs (10, 20,30), batch sizes (16, 32), and hyperparameters were tuned to get optimal performance. The overall accuracy of the selected models varied from 94.5 to 97.7%. Among the models, InceptionV3 exhibited superior performance at 30-epoch and 16-batch size with a 97.7% accuracy, 98.5% precision, and 97.8% recall. For this Inception3 model, the type 1 error was obtained as 1.4% and type II error was 0.9%. The effectiveness of the deep learning model presents a clear path towards integrating them with image-based herbicide applicators for precise weed management.

Introduction: We aimed to describe the clinical profile and risk factors for severe disease in adolescents hospitalised with coronavirus disease 2019 (COVID-19). Methods: A retrospective analysis of an admitted cohort of COVID-19 patients was performed at a tertiary hospital in North India. Adolescents aged 12–18 years who were hospitalised during the first wave (March–December, 2020) and the second wave (March–June, 2021) were included. Data on the demographic details, clinical presentation, laboratory parameters, disease severity at admission, treatments received, and in-hospital outcomes were retrieved. Results: The study included 197 adolescents with a median [inter-quartile range (IQR)] age of 15 (13–17) years, of whom 117 (59.4%) were male. Among these, 170 (86.3%) were admitted during the first wave. Underlying co-morbidities were present in nine (4.6%) patients. A total of 60 (30.9%) patients were asymptomatic. In the severity grading, 148 (84.6%) had mild, 16 (9.1%) had moderate, and 11 (6.3%) had severe disease. Fever (14.9%) and cough (14.9%) were the most commonly encountered symptoms. The median (IQR) duration of hospital stay was 10 (8–13) days, and six (3.1%) patients died in the hospital. Conclusion: Adolescents admitted with COVID-19 had predominantly asymptomatic or mild disease, and the mortality rate was 3.1%.

Long-term (13 years) impact of organic and conventional farming on soil physical properties was evaluated under a rice (Oryza sativa L.) – wheat (Triticum aestivum L.) cropping system in a sandy clay loam soil at New Delhi. The treatments included unfertilized control (T1), conventionally managed (T2) and five organic treatments (T3: FYM @ 10 t/ha to rice and wheat, T4: SGM for rice and LGLM for wheat, T5: SGM + BGA for rice and LGLM + Azotobacter for wheat, T6: SGM + FYM for rice and LGLM + FYM for wheat, and T7: SGM + FYM + BGA for rice and LGLM + FYM + Azotobacter for wheat). The application of organic amendments had positive influence on soil physical properties such as saturated hydraulic conductivity (Ks), field capacity (FC) and permanent wilting point (PWP), plant-available water capacity (PAWC), water holding capacity (WHC), distribution of aggregate size fractions, bulk density (Db), and porosity in the 0-15 and 15-30 cm soil layer. A favourable effect of organic amendments was also found on soil strength (at depth 0-70 cm). Even, values related to these properties were significantly affected by the kinds of organic amendments. But, T1 and T2 had no improvement in soil physical properties over the organic ones. Value of Ks decreased with increase in soil depths. T7 and T3 were superior in Ks to other treatments for 0-15 and 15-30 cm soil depths. Volumetric moisture content at FC (θFC) and PWP (θWP) increased with addition of organic amendments and got varying response due to the kinds of organic amendments or their combinations. θFC and θWP were decreased substantially under T1 and T2, because of no addition of organic inputs. As a result of θFC and θWP, PAWC had also signified an improvement under the influence of different organic amendments over T1 and T2. Generally (for both depths), PAWC of T3, T6 and T7 were 17-26% more than T1, T2, T4 and T5. WHC of upper soil layer (0-15 cm) was greater for organic amended plots than plots devoid of organic inputs and lower values were recorded for 15-30 cm soil layer. WHC of T3, T7 and T6 was highest in comparison to plots amended only with (without FYM) green manure, BGA or Azotobacter as well as T1 and T2 for both depths (0-15 and 15-30 cm). A better soil aggregation was observed under organic amended plots for large and small macro-aggregates (LM - >2 and SM-0.25-2 mm) in both soil depths. Lowest value of Db with highest percentage of pore space was noted under T7 than other treatments in both soil layers. Soil strength was significantly lower in organic amended plots than T1 and T2. This study clearly indicated that application of organic inputs especially FYM, improved the soil physical properties, which resulted in better soil aggregation, porosity and soil moisture retention.

The continuing decline in water resources under the ever-changing climate compels us to re-orient our focus to a more sustainable practice. This study investigates the performance of Triticum aestivum wheat genotypes viz. HD-2967, HD-3086, HD-3249, DBW-187, and HD-3226 under well- and deficit-watered conditions for their root-traits, biomass and nitrogen accumulation and remobilization, and water use efficiencies, grown in PVC-tubes. The genotypes HD-2967, HD-3086, HD-3249, DBW-187, and HD-3226 under well-watered (WW) resulted in 36, 35, 38, 33, and 42% more grain yield compared to deficit-watered (DW). Among the genotypes, HD-3249 had the highest grain yield under both well- and deficit-watered conditions. Compared to DW, the WW had 28%, 30%, and 28% greater root length, biomass, and root length density at flowering {102 days (d), Z61}, while among the genotypes, HD-3249 had relatively greater root-traits. At flowering (Z61) and maturity (132 d, Z89), genotypes under WW accumulated 30-46% and 30-53%, respectively greater shoot biomass over the DW. Furthermore, the shoot biomass remobilised for HD-2967, HD-3086, HD-3249, DBW-187, and HD-3226 under the WW was 32, 37, 39, 35, and 35% greater than the DW. The nitrogen partitioning to different plant parts at flowering (Z61) and maturity (Z89) was significantly greater with the WW than with DW. The total nitrogen- remobilized and contribution to grain-N under the WW was 55, 58, 52, 53, 58% and 9, 19, 15, 17, 17% greater than the DW for the genotypes HD-2967, HD-3086, HD-3249, DBW-187, and HD-3226. The irrigation water use efficiency (WUE) at flowering (Z61) was more under the deficit-watered, but the biomass and grain total WUE was improved with the well-watered condition. Hence, it is apparent that proper scheduling of irrigation and N applications, along with the adoption of a genotype suited to a particular environment, will result in better WUE and grain yields, along with better utilization of scarce resources.

Bitter gourd is an important vegetable crop grown throughout the tropics mainly because of its high nutritional value. Sex expression and identification of gynoecious trait in cucurbitaceous vegetable crops has facilitated the hybrid breeding programme in a great way to improve productivity. In bitter gourd, gynoecious sex expression is poorly reported and detailed molecular pathways involve yet to be studied. The present experiment was conducted to study the inheritance, identify the genomic regions associated with gynoecious sex expression and to reveal possible candidate genes through QTL-seq. Segregation for the gynoecious and monoecious sex forms in the F2 progenies indicated single recessive gene controlling gynoecious sex expression in the genotype, PVGy-201. Gynoecious parent, PVGy-201, Monoecious parent, Pusa Do Mausami (PDM), and two contrasting bulks were constituted for deep-sequencing. A total of 10.56, 23.11, 15.07, and 19.38 Gb of clean reads from PVGy-201, PDM, gynoecious bulk and monoecious bulks were generated. Based on the ΔSNP index, 1.31 Mb regions on the chromosome 1 was identified to be associated with gynoecious sex expression in bitter gourd. In the QTL region 293,467 PVGy-201 unique variants, including SNPs and indels, were identified. In the identified QTL region, a total of 1019 homozygous variants were identified between PVGy1 and PDM genomes and 71 among them were non-synonymous variants (SNPS and INDELs), out of which 11 variants (7 INDELs, 4 SNPs) were classified as high impact variants with frame shift/stop gain effect. In total twelve genes associated with male and female gametophyte development were identified in the QTL-region. Ethylene-responsive transcription factor 12, Auxin response factor 6, Copper-transporting ATPase RAN1, CBL-interacting serine/threonine-protein kinase 23, ABC transporter C family member 2, DEAD-box ATP-dependent RNA helicase 1 isoform X2, Polygalacturonase QRT3-like isoform X2, Protein CHROMATIN REMODELING 4 were identified with possible role in gynoecious sex expression. Promoter region variation in 8 among the 12 genes indicated their role in determining gynoecious sex expression in bitter gourd genotype, DBGy-1. The findings in the study provides insight about sex expression in bitter gourd and will facilitate fine mapping and more precise identification of candidate genes through their functional validation.

Pulses are an important source of energy and protein, essential amino acids, dietary fibers, minerals, and vitamins, and play a significant role in addressing global nutritional security. The global pulse area, production, and average productivity increased from 1961 to 2020 (60 years). Pulses are usually grown under rainfed, highly unstable, and complex production environments, with substantial variability in soil and environmental factors, high year-to-year output variability, and variation in soil moisture. Since the last six decades, there is not much satisfactory improvement in the yield of pulses because of their cultivation in harsh environments, coupled with their continuous ignorance of the farmers and governments in policy planning. As a result, the global food supplies through pulses remained negligible and amounted to merely ~1.0% of the total food supply and 1.2% of the vegan food system. In this situation, protein-rich food is still a question raised at the global level to make a malnutrition-free world. Pulses are a vital component of agricultural biological diversity, essential for tackling climate change, and serve as an energy diet for vegetarians. Pulses can mitigate climate change by reducing the dependence on synthetic fertilizers that artificially introduce nitrogen (N) into the soil. The high demand and manufacture of chemical fertilizers emit greenhouse gases (GHGs), and their overuse can harm the environment. In addition, the increasing demand for the vegetal protein under most global agroecosystems has to be met with under a stressed rainfed situation. The rainfed agroecosystem is a shelter for poor people from a significant part of the globe, such as Africa, South Asia, and Latin America. Nearly, 83% [over 1,260 million hectares (ha)] of cultivated land comes under rainfed agriculture, contributing significantly to global food security by supplying over 60% of the food. In rainfed areas, the limitation of natural resources with the shrinking land, continuous nutrient mining, soil fertility depletion, declining productivity factor, constantly depleting water availability, decreasing soil carbon (C) stock, augmented weed menace, ecological instability, and reduced system productivity are creating a more challenging situation. Pulses, being crops of marginal and semi-marginal soils of arid and semi-arid climates, require less input for cultivation, such as water, nutrients, tillage, labor, and energy. Furthermore, accommodation of the area for the cultivation of pulses reduces the groundwater exploitation, C and N footprints, agrochemical application in the cropping systems, and ill effects of climate change due to their inherent capacity to withstand harsh soil to exhibit phytoremediation properties and to stand well under stressed environmental condition. This article focuses on the role of pulses in ecological services, human wellbeing, soil, environmental health, and economic security for advanced sustainability. Therefore, this study will enhance the understanding of productivity improvement in a system-based approach in a rainfed agroecosystem through the involvement of pulses. Furthermore, the present study highlighted significant research findings and policy support in the direction of exploring the real yield potential of pulses. It will provide a road map to producers, researchers, policymakers, and government planners working on pulses to promote them in rainfed agroecosystems to achieve the United Nations (UN's) Sustainable Development Goals (SDGs).

Various faulty farming practices and low-performance cultivars selection are reducing crop yields, factor productivity, and soil fertility. Therefore, there is an urgent need to achieve better nutrient dynamics and sustainable production by selecting more nutrient-responsive cultivars using efficient nutrient management. The present experiment aimed to enhance crop productivity, seed quality, nutrient efficiency, and soil nutrient dynamics through efficient nutrient management under different lentil cultivars. The experiment was laid out in a split-plot design, assigning three cultivars (viz. Sapna, Garima, and HM-1) in the main plots and ten nutrient management practices in the sub-plots, replicating them thrice. Results revealed that cultivar HM-1 recorded significantly higher seed yield (1.59-1.61 Mg ha-1) and the uptake of N (67.2-67.6 kg ha-1), P (6.8-7.0 kg ha-1), K (13.8-13.9 kg ha-1), Zn (60.4-61.1 g ha-1), and Fe (162.5-165.2 g ha-1) in seed compared to Sapna and Garima. Also, the cultivar HM-1 was more efficient in terms of partial factor productivity for NPK (PFP; 24.27-24.59 kg kg-1), partial nutrient balance (PNB; 2.09-2.13 kg kg-1) and internal utilisation efficiency (IUE; 11.64-11.85 kg kg-1). The study showed that the lentil cultivar HM-1 could be successfully grown by substituting 50% RDN with organic manures, i.e., vermicompost, without compromising crop productivity and soil fertility, thereby sustaining soil-human-environment health.

Present study was carried out during 2018–19 and 2019–20 at ICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan to evaluate the effect of nitrogen (N) and sulphur (S) nutrition on production efficiency, water productivity, and economics of Indian mustard [Brassica juncea (L.) Czern & Coss.] under irrigated conditions. The experiment was conducted in a split plot design with 5 nitrogen levels (0, 60, 80, 100 and 120 kg/ha) as the main plot treatment and 4 sulphur levels (0, 20, 40, and 60 kg/ha) as subplot treatment with 3 replications. Results revealed that the application of 120 kg N/ha recorded the highest plant height (205 cm), leaf area index (5.4), number of leaves/ plant (47.3), primary branches/plant (6.8), secondary branches/plant (18.9), SCMR (46.5), number of siliquae/plant (382), number of seeds/silique (18), stem girth (6.65 cm), test weight (5.79 g) and seed yield (3.04 t/ha). Among the different S levels, the maximum values of growth and physiological parameters, yield attributes and seed yield were recorded at 60 kg S/ha (2.71 t/ha). Similarly, production efficiency (22.1 kg/ha/day) and water productivity (1.53 kg seed/m3) were found higher than 120 kg N/ha and 60 kg S/ha. The highest net monetary returns and Benefit cost (B: C) ratio were recorded with 120 kg N/ha and 60 kg S/ha.Thus, the application of N and S may be revised to 120 and 60 kg/ha, respectively for Indian mustard in the irrigated semi-arid climates to achieve higher productivity, efficiency and profitability.

OBJECTIVES:. Our objective was to compare norepinephrine plus dobutamine versus epinephrine as the first-line agent in children with fluid refractory cold septic shock. DESIGN:. Open-label randomized controlled study. SETTING:. A single-center PICU from North India. PATIENTS:. Children 2 months to less than 18 years old with fluid refractory cold septic shock. INTERVENTIONS:. In the intervention group, norepinephrine and dobutamine were started and in the control group, epinephrine was started as the first-line vasoactive agent. The primary outcome was the proportion attaining shock resolution (attaining all the therapeutic endpoints) at 1 hour of therapy. MEASUREMENTS AND MAIN RESULTS:. We enrolled 67 children: 34 in the norepinephrine plus dobutamine group (intervention) and 33 in the epinephrine group (control). There was no difference in shock resolution at 1 hour (17.6% vs 9%; risk ratio [RR], 2.0; 95% CI, 0.54–7.35; p = 0.25), 6 hours (76.4% vs 54.5%; RR, 1.69; 95% CI, 0.92–3.13; p = 0.06), and 24 hours between the intervention and control groups, respectively. Children in the norepinephrine plus dobutamine group attained shock resolution earlier (measured from starting of vasoactive agents to attaining all the therapeutic endpoints) (hazard ratio, 1.84 [1.1–3.08]). The difference in 28-day mortality was not significant (23.5% vs 39.3% in the intervention and control groups, respectively [RR, 0.59; 95% CI, 0.28–1.25]). CONCLUSIONS:. In children with fluid refractory cold septic shock, with use of norepinephrine plus dobutamine as first-line agents, the difference in the proportion of children attaining shock resolution at 1 hour between the groups was inconclusive. However, the time to shock resolution was earlier in the norepinephrine plus dobutamine group. Also, fewer children in the intervention group were refractory to treatment. Further studies powered to detect (or exclude) an important difference would be required to test this intervention.

In the current scenario, it is estimated that by 2050, there will be an additional 2.5 billion people and a 70% increase in food demand. Crop yields are not increasing fast enough to support global needs, and world agriculture is facing several serious challenges. Therefore, insects can be a nutritious alternative to meet the ever-increasing food demand in the present and future. The majority of insect consumption occurs in developing countries, with approximately 1,900 insect species consumed worldwide. Food and feed derived from them are of high quality, have a high feed conversion ratio and emit a low level of greenhouse gases. Among insects silkworms are beneficial to humans, not only because of their high nutritional value, but also because of their several pharmacological properties. Silkworm eggs, larvae, and pupae contains high amount of proteins, oils, minerals, vitamins, and several other beneficial components which are nutritious as well as have positive effect on human health. Studies have shown that silkworm pupae protect the liver, enhance immunity, inhibit apoptosis, inhibit cancer, inhibit tumor growth, inhibit microbial growth, regulate blood glucose and blood lipids, and lower blood pressure. This review paper summerized the nutritional value of different life stages of silkworm, nutritional comparison of silkworm with the major human foods, and the effects of silkworm consumption on human health, thus ittargets to generate interest toward in sericulture and improve human health by using silkworm as a nutritious food and attain sustainability in food and nutritional security.

Flocculated suspended sediments (flocs) are found in a variety of environments globally, and their transport and behavior bear substantial importance to several industries including fisheries, aquaculture, and shipping. Additionally, the modelling of their behavior is important for estuarine and coastal flood prediction and defence, and the process of flocculation occurs in other unrelated industries such as paper and chemical production. Floc porosity is conventionally assessed using inferential indirect or proxy data approaches. These methods underestimate floc porosity % by c. 30% and cannot measure the micro-scale complexity of these pore spaces and networks, rendering inputs to models sub-optimal. This study introduces a novel 3D porosity and pore space quantification protocol, that produces directly quantified porosity % and pore space data. • 3D floc data from micro-CT scanning is segmented volumetrically • This segmented volume is quantified to extract porosity and several pore space parameters from the floc structure

Abstract Conservation agriculture (CA)-based practices have been promoted and recouped, as they hold the potential to enhance farm profits besides a consistent improvement in soil properties. A 7 years' field experiment consisting of three crop establishment practices viz., zero-till flatbed (ZTFB), permanent beds (PNB), conventional system (CT) along with the three-nutrient management; nutrient expert-based application (NE), recommended fertilization (RDF), and farmers’ fertilizer practice (FFP), was carried out from 2013 to 2020. The CA-based practices (ZTFB/PNB) produced 13.9–17.6% greater maize grain-equivalent yield (MGEY) compared to the CT, while NE and RDF had 10.7–20% greater MGEY than the FFP. PNB and ZTFB gave 28.8% and 24% additional net returns than CT, while NE and RDF had 22.8% and 17.4% greater returns, respectively over FFP. PNB and ZTFB had 2.3–4.1% (0.0–0.20 m soil layers) lower bulk density than the CT. Furthermore, microbial biomass carbon (MBC) increased by 8–19% (0.0–0.50 m soil layers) in ZTFB/PNB over the CT, and by 7.6–11.0% in NE/RDF over FFP. Hence, CA-based crop establishment coupled with the NE or RDF could enhance the yields, farm profits, soil properties of the maize–chickpea rotation, thereby, could sustain production in the long run.

In the most productive area of the Indo-Gangetic Plains in Northwest India where high yields of rice and wheat are commonplace, a medium-term cropping system trial was conducted in Haryana State. The goal of the study was to identify integrated management options for further improving productivity and profitability while rationalizing resource use and reducing environmental externalities (i.e., "sustainable intensification", SI) by drawing on the principles of diversification, precision management, and conservation agriculture. Four scenarios were evaluated: Scenario 1 - "business-as-usual" [conventional puddled transplanted rice (PTR) followed by (fb) conventional-till wheat]; Scenario 2 - reduced tillage with opportunistic diversification and precision resource management [PTR fb zero-till (ZT) wheat fb ZT mungbean]; Scenario 3 - ZT for all crops with opportunistic diversification and precision resource management [ZT direct-seeded rice (ZT-DSR) fb ZT wheat fb ZT mungbean]; and Scenario 4 - ZT for all crops with strategic diversification and precision resource management [ZT maize fb ZT wheat fb ZT mungbean]. Results of this five-year study strongly suggest that, compared with business-as-usual practices, SI strategies that incorporate multi-objective yield, economic, and environmental criteria can be more productive when used in these production environments. For Scenarios 2, 3, and 4, system-level increases in productivity (10-17%) and profitability (24-50%) were observed while using less irrigation water (15-71% reduction) and energy (17-47% reduction), leading to 15-30% lower global warming potential (GWP), with the ranges reflecting the implications of specific innovations. Scenario 3, where early wheat sowing was combined with ZT along with no puddling during the rice phase, resulted in a 13% gain in wheat yield compared with Scenario 2. A similar gain in wheat yield was observed in Scenario 4 vis-à-vis Scenario 2. Compared to Scenario 1, wheat yields in Scenarios 3 and 4 were 15-17% higher, whereas, in Scenario 2, yield was either similar in normal years or higher in warmer years. During the rainy (kharif) season, ZT-DSR provided yields similar to or higher than those of PTR in the first three years and lower (11-30%) in Years 4 and 5, a result that provides a note of caution for interpreting technology performance through short-term trials or simply averaging results over several years. The resource use and economic and environmental advantages of DSR were more stable through time, including reductions in irrigation water (22-40%), production cost (11-17%), energy inputs (13-34%), and total GWP (14-32%). The integration of "best practices" in PTR in Scenario 2 resulted in reductions of 24% in irrigation water and 21% in GWP, with a positive impact on yield (0.9 t/ha) and profitability compared to conventional PTR, demonstrating the power of simple management changes to generate improved SI outcomes. When ZT maize was used as a diversification option instead of rice in Scenario 4, reductions in resource use jumped to 82-89% for irrigation water and 49-66% for energy inputs, with 13-40% lower GWP, similar or higher rice equivalent yield, and higher profitability (27-73%) in comparison to the rice-based scenarios. Despite these advantages, maize value chains are not robust in this part of India and public procurement is absent. Results do demonstrate that transformative opportunities exist to break the cycle of stagnating yields and inefficient resource use in the most productive cereal-based cropping systems of South Asia. However, these SI entry points need to be placed in the context of the major drivers of change in the region, including market conditions, risks, and declining labor availability, and matching with the needs and interests of different types of farmers.

Conventional- till (CT) agriculture is known to be ecologically indiscreet, economically and environmentally unsustainable, and leads to degradation of soil and environment in Indo-Gangetic Plains (IGP). Conservation Agriculture (CA) approach was introduced to manage agro-ecosystems for improved and sustained productivity, and increased farmers’ profits while maintaining the natural resources. This comprises the management of natural resources at the farm, village, and landscape scales to increase synergies between food production and ecosystem conservation. CA-based rice-wheat (RW) system integrated with mungbean improved the system productivity by ∼10%, profitability by 20–30% using 15–30% less irrigation water, and 20–25% less energy input compared to conventional RW system in the IGP. However, the replacement of rice with maize improved the productivity by 10-15% and profitability by 40–50% using ∼70% less irrigation water. CA layered with subsurface drip irrigation (SDI) in CA-based rice/maize systems recorded ∼5% higher system productivity and saved ∼50% of irrigation water compared to flood irrigation in CT-based systems. CA-based systems are found more adapted to extreme climatic conditions and can mitigate the negative effects of climatic stresses like terminal heat, water stress and thereby helps in increasing crop yields to the tune of 0.4–0.8 t ha−1 per season over the conventional system.

Abstract Reduction of excess nutrient application and balanced fertilizer use are the key mitigation options in agriculture. We evaluated Nutrient Expert (NE) tool-based site-specific nutrient management (SSNM) in rice and wheat crops by establishing 1594 side-by-side comparison trials with farmers’ fertilization practices (FFP) across the Indo-Gangetic Plains (IGP) of India. We found that NE-based fertilizer management can lower global warming potential (GWP) by about 2.5% in rice, and between 12 and 20% in wheat over FFP. More than 80% of the participating farmers increased their crop yield and farm income by applying the NE-based fertilizer recommendation. We also observed that increased crop yield and reduced fertilizer consumption and associated greenhouse gas (GHG) emissions by using NE was significantly influenced by the crop type, agro-ecology, soil properties and farmers’ current level of fertilization. Adoption of NE-based fertilizer recommendation practice in all rice and wheat acreage in India would translate into 13.92 million tonnes (Mt) more rice and wheat production with 1.44 Mt less N fertilizer use, and a reduction in GHG of 5.34 Mt CO2e per year over farmers’ current practice. Our study establishes the utility of NE to help implement SSNM in smallholder production systems for increasing crop yields and farmers’ income while reducing GHG emissions.

Abstract Renal stones constitute 40% of renal disorders in Pakistan with silent stones constituting up to 3%. Nephrolithotomy and Pyelolithotomy were the only surgical options available for the treatment of large renal stones, with high rate of complications. Percutaneous nephrolithotomy (PCNL) has now become the mainstay of treatment for large renal stones over the past 30 years. Recent advances in equipment and technology has made PCNL the gold standard for this disease, resulting in removal of stones with shorter recovery time and decreased morbidity and mortality, and with few complications Case Discussion A boy aged 13 years, underwent mini PCNL for treatment of left side renal stone causing hydronephrosis, previously evaluated by KUB X-Rray and non contrast CT scan. His retrograde-percutanous access to the collecting system was done under fluoroscopic guidance. At the end of procedure, patient revealed taut and distended abdomen. Aspiration revealed presence of intraperitoneal fluid. Patient was effectively treated with immediate placement of abdominal drain with improvement of clinical presentation Conclusion Hydroperitoneum is a rare complication of conventional PCNL. Based on our experience and review of published literature, our case of hydroperitoneum after mini PCNL, is the first of its kind. A high degree of sensitivity and knowledge of this complication during PCNL in children would help identify and manage this complication in future. We recommend examination of abdomen post-PCNL in every child before he/she is brought out of anesthesia.

Purpose – Increasing trends of climatic risk pose challenges to the food security and livelihoods of smallholders in vulnerable regions, where farmers often face loss of the entire crop, pushing farmers (mostly men) out of agriculture in destitution, creating a situation of agricultural making agriculture highly feminization and compelling male farmers to out-migrate. Climate-smart agricultural practices (CSAPs) are promoted to cope with climatic risks. This study aims to assess how knowledge related to CSAPs, male out-migration, education and income contribute to the determinants of male out-migration and CSAPs adoption and how they respond to household food security. Design/methodology/approach – Sex-disaggregated primary data were collected from adopter and non-adopter farm families. STATA 13.1 was used to perform principle component analysis to construct knowledge, yield and income indices. Findings – Yield and income index of adopters was higher for men than women. The probability of out-migration reduced by 21% with adoption of CSAPs. An increase in female literacy by 1 unit reduces log of odds to migrate by 0.37. With every unit increase in knowledge index, increase in log-odds of CSAPs adoption was 1.57. Male:female knowledge gap was less among adopters. Non-adopters tended to reduce food consumption when faced with climatic risks significantly, and the probability of migration increased by 50% with a one-unit fall in the nutrition level, thus compelling women to work more in agriculture. Gender-equitable enhancement of CSAP knowledge is, therefore, key to safeguarding sustainable farming systems and improving livelihoods. Social implications – The enhancement of gender equitable knowledge on CSAPs is key to safeguard sustainable farming systems and improved livelihoods. Originality/value – This study is based on the robust data sets of 100 each of male and female from 100 households (n = 200) using well-designed and validated survey instrument. From 10 randomly selected climate-smart villages in Samastipur and Vaishali districts of Bihar, India, together with focus group discussions, the primary data were collected by interviewing both men and women from the same household.

Abstract Cellular senescence is a stable cell cycle arrest that normal cells undergo after a finite number of divisions, in response to a variety of intrinsic and extrinsic stimuli. Although senescence is largely established and maintained by the p53/p21WAF1/CIP1 and pRB/p16INK4A tumour suppressor pathways, the downstream targets responsible for the stability of the growth arrest are not known. We have employed a stable senescence bypass assay in conditionally immortalised human breast fibroblasts (CL3EcoR) to investigate the role of the DREAM complex and its associated components in senescence. DREAM is a multi-subunit complex comprised of the MuvB core, containing LIN9, LIN37, LIN52, LIN54, and RBBP4, that when bound to p130, an RB1 like protein, and E2F4 inhibits cell cycle-dependent gene expression thereby arresting cell division. Phosphorylation of LIN52 at Serine 28 is required for DREAM assembly. Re-entry into the cell cycle upon phosphorylation of p130 leads to disruption of the DREAM complex and the MuvB core, associating initially to B-MYB and later to FOXM1 to form MMB and MMB-FOXM1 complexes respectively. Here we report that simultaneous expression of MMB-FOXM1 complex components efficiently bypasses senescence with LIN52, B-MYB, and FOXM1 as the crucial components. Moreover, bypass of senescence requires non-phosphorylated LIN52 that disrupts the DREAM complex, thereby indicating a central role for assembly of the DREAM complex in senescence.

Introduction: The Coronavirus Disease-19 (COVID-19) pandemic caused by 2019 novel coronavirus was first reported in Wuhan, China. It is a disease of the respiratory system with clinical presentation of fever, cough, breathlessness, headache, fatigue etc. Transmission route is through person to person contact and through direct contact with respiratory droplets generated when an infected person coughs or sneezes. In such cases usage of facemasks is very crucial. At the same time awareness regarding the same in the community is very important. Hence, through this survey authors have assessed and recorded the awareness about facemasks and its usage in the community in the Indian population along with the basic hygiene precautions taken by the people. Aim: To conduct a detailed survey regarding the awareness and usage of facemask in the rural population in Maharashtra. Materials and Methods: The study was a three month crosssectional online survey conducted by Maharashtra Academy of Engineering Education and Research’s (MAEER’s) Talegaon Dabhade Physiotherapy College in the rural community of western Maharashtra, India, from October to December 2020. A self-administered online questionnaire consisting of 20 questions was prepared regarding the awareness about masks usage and proper methods to be considered with reference to the guidelines given by World Health Organisation (WHO), Indian Council of Medical Research (ICMR), Centers for Disease Control (CDC) and Prevention and literature reviews. The forms were shared on social media platforms such as WhatsApp, Facebook, and Instagram using Google forms. Statistical analysis was done by descriptive analysis using percentage. Results: Out of total 390 participants, 210 (53.84%) were males and 180 (46.15%) were females. Considering the knowledge about the types of masks available was 67.1%, self-education was 88%. A 44% of the population thinks that one can wear a facemask up to eight hours if worn properly. Similarly knowledge about disinfecting the mask before throwing was 86%. Active efforts to maintain good hygiene by washing hands was 90%. Also, 55% of the population avoids touching their mask frequently. In practice, 56.1% of the population avoids wearing a mask while walking or jogging. Varieties of masks are being used by the people depending on the cost and availability. Conclusion: The coronavirus pandemic has evoked a sense of awareness about self-care, hygienic habits and being updated about one’s health. Further work can be done on cost effectiveness and availability of the good quality masks.