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MicroRNAs (miRNAs) are kind of small non-coding RNAs that negatively regulate gene expression at post-transcription level, and those non-coding RNAs appear to play a key role in tumorigenesis. The aim of this study was to investigate the biological role of miR-96 in papillary thyroid carcinoma (PTC) cell lines. We identified miR-96 to be up-regulated in PTC specimens in comparison to matched normal tissues by microRNA microarray and RT-qPCR analysis (P < 0.05). Next, to explore the potential function of miR-96, PTC cell lines K1 and TPC1 were transiently transfected with miR-96 mimics and inhibitor. Successful transfection being confirmed by RT-qPCR. Ectopic expression of miR-96 promoted proliferation and colony formation ability, and inhibited apoptosis of K1 and TPC1 cells, whereas down-regulated expression of miR-96 suppressed those functions when compared with the control cells. According to a computational prediction, FOXO1 maybe a potential target of miR-96. Luciferase assays revealed that miR-96 is directly targeted to both binding sites of FOXO1 3’-untranslated region (3’-UTR) and suppressed the FOXO1 expression, and subsequently inhibited the expression of Bim protein in PTC cells. Moreover, the expression of FOXO1 had an inverse correlation with expression of miR-96 in PTC specimens by RT-qPCR and western blot analysis. The data from the present study demonstrated that miR-96 can promote proliferation, and inhibit apoptosis in PTC cell lines K1 and TPC1, thus miR-96 may play an oncogenic role in PTC by inhibiting the FOXO1 and regulating AKT/FOXO1/Bim pathway, and it may serve as a novel therapeutic target for miRNA-based PTC therapy.

CDK8 is a cyclin-dependent kinase (CDK) member of the mediator complex that couples transcriptional regulators to the basal transcriptional machinery, and it has been investigated for possible tumor promoting functions. However, it is unclear whether CDK8 is involved in breast tumor cells growth. The aim of this study was to determine whether the suppression of CDK8 by small interfering RNA (siRNA) inhibits the growth of human breast cancer cell. Methods: CDK8-siRNA transfection was used to silencing the CDK8 gene in established breast cancer cell line, MDA-MB-231 and MCF-7, successful transfection being confirmed by Real-time PCR and could be shown by Western Blotting. CDK8 deletion caused significant decline in cell proliferation was observed in breast cancer cell lines as investigated by MTS assay, the number and size of the colonies formed were also significantly reduced in the absence of CDK8. Furthermore, transwell test were conducted to explore the migration of breast cancer cells. Moreover CDK8 gene knockdown arrested cell cycle. Results: CDK8 mRNA expression was reduced after transfection with CDK8-siRNA, and protein expression had a similar trend. Transfection of CDK8-siRNA suppressed breast cancer cells proliferation and migration; meanwhile the cells were arrested at G0/G1 phase. Conclusions: CDK8 plays an essential role in breast cancer progression, which might inhibit the proliferation and migration in breast cancer cells.

In order to deal with the shortcoming of poor light effect in the scene control, a new intelligent lighting control system was designed based on the CIE gamut standard and the dual-CPU Embedded System Architecture. By employing light adjustment of the multi-colored LED, color control and scene settings of the assembled light, the intelligent lighting control system with the graphic user interface and the touch-control technology is designed and realized. Besides the easy operation, the flexible and reliable control makes the system widely used in the entertainment places, such as hotel, chamber, office building, urban landscape, etc

The flow of air entering engine cylinders is controlled by the electronic throttle, which is an important part of automotive engines and has a direct effect on the fuel efficiency and the security of automobiles. For the demand of quality sampling, an electronic throttle performance test system, which met the manufacturers’ requirements, was designed to achieve the test of the opening performance of electronic throttle on the bases of C++ Builder, NI’s PCI-6259 data acquisition card and PID throttle controller. At present, the system has been put into use in the electronic throttle factory. Compared with original test devices, the system is of more stability and higher accuracy, furthermore, more than 40% of the test time is saved.