201. Identification and analysis of polyaromatic hydrocarbons (PAHs)--biodegrading bacterial strains from refinery soil of India
- Author
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Anil Kumar Saxena, Harmesh Sahay, Lata Nain, Shashi Bala Singh, Richa Sharma, Priyanka Chaudhary, and Alok Kumar Pandey
- Subjects
India ,Bacillus ,Management, Monitoring, Policy and Law ,Biology ,Serratia ,Extraction and Processing Industry ,Microbiology ,chemistry.chemical_compound ,Soil ,Bioremediation ,Soil Pollutants ,Petroleum Pollution ,Polycyclic Aromatic Hydrocarbons ,Soil Microbiology ,General Environmental Science ,Fluorenes ,Pyrenes ,Bacteria ,Brevibacterium ,General Medicine ,Phenanthrene ,Acinetobacter ,Phenanthrenes ,biology.organism_classification ,Pollution ,Biodegradation, Environmental ,chemistry ,Environmental chemistry ,Stenotrophomonas ,Alcaligenes ,Soil microbiology ,Environmental Monitoring - Abstract
Polyaromatic hydrocarbons (PAHs) utilizing bacteria were isolated from soils of seven sites of Mathura refinery, India. Twenty-six bacterial strains with different morphotypes were isolated. These strains were acclimatized to utilize a mixture of four polycyclic aromatic hydrocarbons, i.e., anthracene, fluorene, phenanthrene, and pyrene, each at 50 mg/L concentration as sole carbon source. Out of total isolates, 15 potent isolates were subjected to 16S rDNA sequencing and identified as a member of diverse genera, i.e., Bacillus, Acinetobacter, Stenotrophomonas, Alcaligenes, Lysinibacillus, Brevibacterium, Serratia, and Streptomyces. Consortium of four promising isolates (Acinetobacter, Brevibacterium, Serratia, and Streptomyces) were also investigated for bioremediation of PAH mixture. This consortium was proved to be efficient PAH degrader resulting in 40-70 % degradation of PAH within 7 days. Results of this study indicated that these genera may play an active role in bioremediation of PAHs.
- Published
- 2015