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1,727 results on '"Li, Dongfang"'

224. Commentary of WHO classification in odontogenic tumours (2017 edition)

Author

JI Li, ZHU Zhinong, LIAN Wenwei, and LI Dongfang

Subjects
Classification of tumors, Odontogenic tumors, Odontogenic cysts, Pathology, Molecular genet⁃ ics, World Health Organization, Medicine
Abstract

The 4th edition of WHO Classification of Head and Neck Tumours has been published in Jan. 2017. In Chapter 8‘Odontogenic and maxillofacial bone tumours’, the classification of odontogenic tumors was recomposed from the previous edition in 2005. In this paper, we introduced the modification of odontogenic tumors classification between 2017 and 2005 in a comprehensive discussion.

Published
2018
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234. Long-term corneal recovery by simultaneous delivery of hPSC-derived corneal endothelial precursors and nicotinamide

Author

Li, Zongyi, Duan, Haoyun, Jia, Yanni, Zhao, Can, Li, Wenjing, Wang, Xin, Gong, Yajie, Dong, Chunxiao, Ma, Bochao, Dou, Shengqian, Zhang, Bin, Li, Dongfang, Cao, Yihai, Xie, Lixin, Zhou, Qingjun, and Shi, Weiyun

Subjects
Care and treatment, Development and progression, Patient outcomes, Methods, Health aspects, Corneal diseases -- Development and progression -- Care and treatment, Niacinamide -- Health aspects, Stem cell transplantation -- Methods -- Patient outcomes, Stem cells -- Transplantation
Abstract

Introduction Corneal endothelium maintains corneal hydration and transparency through its barrier and pump function. When the endothelial cell density diminishes to fewer than 500 cells/[mm.sup.2] due to dystrophy, trauma, or [...], Human pluripotent stem cells (hPSCs) hold great promise for the treatment of various human diseases. However, their therapeutic benefits and mechanisms for treating corneal endothelial dysfunction remain undefined. Here, we developed a therapeutic regimen consisting of the combination of hPSC-derived corneal endothelial precursors (CEPs) with nicotinamide (NAM) for effective treatment of corneal endothelial dysfunction. In rabbit and nonhuman primate models, intracameral injection of CEPs and NAM achieved long-term recovery of corneal clarity and thickness, similar with the therapeutic outcome of cultured human corneal endothelial cells (CECs). The transplanted human CEPs exhibited structural and functional integration with host resident CECs. However, the long-term recovery relied on the stimulation of endogenous endothelial regeneration in rabbits, but predominantly on the replacing function of transplanted cells during the 3-year follow-up in nonhuman primates, which resemble human corneal endothelium with limited regenerative capacity. Mechanistically, NAM ensured in vivo proper maturation of transplanted CEPs into functional CECs by preventing premature senescence and endothelial-mesenchymal transition within the TGF-[beta]-enriched aqueous humor. Together, we provide compelling experimental evidence and mechanistic insights of simultaneous delivery of CEPs and NAM as a potential approach for treating corneal endothelial dysfunction.

Published
2022
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237. Development of immunoassay screening methods using long wavelength fluorescence

Author

Li, Dongfang

Subjects
616.9950756, Immunoassay, FIA, Fluorescence, Immunochromatography
Abstract

The developments of immunoassay methods for the early stage diagnosis of tuberculosis (TB) are described. These went through two different routes, one through flow injection analysis (FIA), and the other using immunochromatography methodology. The design of a simple longwavelength fluorescence detector to serve the above purposes has also been described. The FIA immunoassay methods involve immobilising antibodies on to beads, either directly or through protein A based solid phases. The beads are then packed into a micro-column reactor for incorporation into the FIA system. In this case reactor-bound molecules are eluted from the system by a change of pH, thus limiting the available fluorophores to those that are reasonably fluorescent in acid solution. Sandwich (reagent excess) assays have been investigated. A couple of long wavelength (600-800) fluorophores have been studied. The bead injection option has also been investigated. The immunochromatographic method uses a lateral flow system and a sandwich (two-site) immunometric assay. Capture antibodies are immobilised on a coated membrane matrix at a pre-determined position and the antigen is analysed after binding to a fluorescence-labelled antibody. Both fluorescent latex preparations and conventional fluorescent labels have been used and compared. The strips are simply immersed in a small volume of sample to start the analysis. The chromatographic step is rapid and extremely simple. The fluorescence detector is fitted with a motor-driven sample holder to allow the length of the immunochromatographic strip to be scanned. The detector utilises a diode laser light source, optical filters in the emission beam and a miniaturised photomultiplier. It can be easily modified for the FIA, and can readily be adapted to operate from batteries, so is suitable for field use.

Published
2004

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