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SUMMARY: Pollen tube integrity, growth and guidance are crucial factors in plant sexual reproduction. Members of the plant Skewed5 (SKU5) Similar (SKS) family show strong similarity to multicopper oxidases (MCOs), but they lack conserved histidines in MCO active sites. The functions of most SKS family members are unknown. Here, we show that Arabidopsis pollen‐expressed SKS11 and SKS12 play important roles in pollen tube integrity, growth and guidance. The sks11sks12 mutant exhibited significantly reduced male fertility. Most of the pollen from sks11sks12 plants burst when germinated, and the pollen tubes grew slowly and exhibited defective growth along the funiculus and micropyle. SKS11‐GFP and SKS12‐mCherry were detected at the cell wall in pollen tubes. The contents of several cell wall polysaccharides and arabinogalactans were decreased in the pollen tube cell walls of sks11sks12 plants. Staining with a reactive oxygen species (ROS)‐sensitive dye and use of the H2O2 sensor HyPer revealed that the ROS content in the pollen tubes of sks11sks12 plants was remarkably reduced. SKS11444His‐Ala, in which the last conserved histidine was mutated, could restore the mutant phenotypes of sks11sks12. Thus, SKS11/12 are required for pollen tube integrity, growth and guidance possibly by regulating the ROS level and cell wall polysaccharide deposition or remodeling in pollen tubes. Significance Statement: Pollen tube integrity, growth and guidance are crucial for plant sexual reproduction. Arabidopsis SKU5 Similar 11 and 12, which are highly expressed in pollen and pollen tubes, function in pollen tube integrity, growth and guidance to ensure successful double fertilization. [ABSTRACT FROM AUTHOR]

The bread with Agaricus bisporus powder has the defects of poor texture and taste, so it is necessary to optimize the appropriate additives in order to improve its quality. The purpose of this study was to evaluate improvement of the combination of vital wheat gluten, sucrose fatty acid esters and cellulase on the improved Agaricus bisporus powder bread (IABPB), with wheat bread (WB) and bread with Agaricus bisporus powder (ABPB) as control. The results of rheological properties indicated the dough samples improved with three improvers had higher solid-like behaviour than the control sample. The results of nutritional quality analysis showed that the protein and dietary fiber content of IABPB was higher than those of WB and ABPB, but the fat content was relatively low. In addition, the additives combination could effectively improve the baking quality of ABPB. Compared with ABPB without additives, the specific volume increased by 21.22%, the brightness of bread crumb increased by 8.75%, but the crumb hardness decreased by 32.57%. Furthermore, the study on texture property and water migration during the storage showed that the addition of three improvers could delay the aging of bread. Therefore, it was feasible to use additives combination as a special quality improver for ABPB, which could effectively improve its quality. [ABSTRACT FROM AUTHOR]

To meet the Chinese government's energy-saving and emission-reduction policies, flexible peak regulation is necessary for traditional coal-fired boilers. Flexible peaking leads to large changes in boiler load, which affects the safety of the boiler water wall. In this paper, a 1000 MW ultra-supercritical unit was tracked for three years, and effective data were selected to study the temperature characteristics of the water wall under flexible peak regulation. The results show that the lower the load, the greater the temperature fluctuation of the water wall. The temperature distribution of the spiral water wall is more uniform. The position of the temperature valley value of the rear spiral water wall was found, and the load of more even temperature distribution was also found. The temperature change of the front vertical water wall was the most complex of all the water walls. The 643.9 MW load case showed different behavior to the temperature distribution of the water wall. The side water walls were heated evenly under the different loads. The characteristics of the temperature distribution of the side vertical water wall were found through statistical analysis. The fitting equation for the change rule of the temperature is presented. The higher the load, the better the equations. Finally, this paper gives some advice on how to avoid temperature deviation in the water wall, and the detailed research highlights the safe running of water walls. [ABSTRACT FROM AUTHOR]

For the position synchronization control problem of multiple nonlinear manipulator systems, a bounded distributed cooperative controller is designed in this paper. Based on a fixed‐time position observer, a distributed finite‐time synchronization controller is designed by using the saturated control technique and homogenous system theory. Compared with the existing results, the proposed distributed cooperative control algorithm not only guarantees finite‐time convergence but also satisfies the input saturation. Finally, some numerical simulation results are presented to verify the feasibility of the theory results with six‐degree‐of‐freedom robots. [ABSTRACT FROM AUTHOR]

To explore the global-scale coupling characteristics between ionospheric disturbances and geomagnetic storms, and research the near-Earth space environment, the time synchronization, correlation, and fitting relationship of geomagnetic field and ionosphere are analyzed in 78 geomagnetic storms from 2013 to 2017. Simultaneously, in order to improve the accuracy of ionospheric disturbance monitoring, a method for detecting ionospheric disturbances based on wavelet transform is proposed. Results show that the signal noise of global electron content is abnormally enhanced in 76% of the geomagnetic storm events, and the abnormal period coincides with the fluctuation period of the geomagnetic index. Two parameters, named Match and non-Match, are proposed to quantify the time synchronization between geomagnetic storms and ionospheric disturbances, it is found that a high synchronization exists between the two. The weighted polynomial function model, which used the ionospheric and geomagnetic parameters, is established. The fitting results show a significant functional relationship between the two, indicating that geomagnetic storm has a significant effect on ionospheric disturbance. The T-test results indicate a significant correlation between the fitted function and the measured data in 86% storm events. [ABSTRACT FROM AUTHOR]

Developmental pluripotency-associated 2 (Dppa2) and developmental pluripotency-associated 4 (Dppa4) as positive drivers were helpful for transcriptional regulation of zygotic genome activation (ZGA). Here, we systematically assessed the cooperative interplay of Dppa2 and Dppa4 in regulating cell pluripotency and found that simultaneous overexpression of Dppa2/4 can make induced pluripotent stem cells closer to embryonic stem cells (ESCs). Compared with other pluripotency transcription factors, Dppa2/4 can regulate majorities of signaling pathways by binding on CG-rich region of proximal promoter (0–500 bp), of which 85% and 77% signaling pathways were significantly activated by Dppa2 and Dppa4, respectively. Notably, Dppa2/4 also can dramatically trigger the decisive signaling pathways for facilitating ZGA, including Hippo, MAPK and TGF-beta signaling pathways and so on. At last, we found alkaline phosphatase, placental-like 2 (Alppl2) was completely silenced when Dppa2 and 4 single- or double-knockout in ESC, which is consistent with Dux. Moreover, Alppl2 was significantly activated in mouse 2-cell embryos and 4–8 cells stage of human embryos, further predicted that Alppl2 was directly regulated by Dppa2/4 as a ZGA candidate driver to facilitate pre-embryonic development. [ABSTRACT FROM AUTHOR]

Recent studies have shown that the combined use of renin angiotensin system inhibitor, SGLT2 inhibitors and/or mineralocorticoid receptor antagonist provides additional renal protection for patients with diabetic kidney disease (DKD). Similarly, in traditional Chinese medicine, the synergistic application of multiple herbs often brings more significant therapeutic effects. However, the synergistic or additive mechanisms of traditional Chinese medicine in combination therapy are not fully understood. In our previous studies, we show that arctigenin (ATG), a major component of Fructus Arctii , attenuates proteinuria and renal injury in diabetic mice by activating PP2A, and puerarin (a class of known isoflavones) can also reduce proteinuria and renal injury in diabetic mice via activation of Sirt1. Here, we further explored the potential additive renal protection of these two compounds in diabetic mice. Research has found that ATG and puerarin have a synergistic effect in reducing albuminuria in db/db mice. Mechanistically, we found that ATG reduced NF-κB p65 phosphorylation likely through activation of PP2A while puerarin reduced p65 acetylation via Sirt1 activation. Therefore, ATG and puerarin have additive inhibitory effects on the NF-κB activation, which is a key inflammatory pathway in DKD. RNA-sequencing analysis revealed distinct pathways activated by ATG and puerarin in the diabetic kidney, which may provide an additional mechanism for their additive effects in DKD. Our study suggests that ATG and puerarin could be a new combination therapy for DKD and reveals its underlined mechanisms. [Display omitted] • Multiple agents are used in combination with unclear mechanisms in TCM. • ATG and puerarin have synergistic effects in reducing albuminuria in db/db mice. • ATG reduced NF-κB p65 phosphorylation while puerarin reduced p65 acetylation. [ABSTRACT FROM AUTHOR]