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Data transformation of the reference soil organic matter (SOM) decomposition rates (kref), often derived as turnover times or in alternative formats, is commonly used to develop ecological models for projecting the persistence of SOM. However, the effects of reciprocal or logarithmic transformation of kref on model performance and edaphic‐climatic patterns remain uncertain. Here, we convert published kref values into reciprocal or logarithmic formats and establish machine learning models between the transformed kref and edaphic‐climatic predictors. We show that models trained with the transformed kref exhibit a 11.6%−68.4% reduction in performance upon re‐conversion to kref compared to those trained with the original kref. The variable importance analysis identifies distinct key predictors governing the original kref and its transformed counterparts. This suggests that data transformation alters the relative significance of predictors without necessarily improving kref prediction performance. Consequently, our study underscores the importance of directly focusing on the original values rather than alternative representations when dissecting a given variable's patterns and mechanisms in ecological modeling. Core Ideas: Data transformation caused reduced predictive performance upon re‐conversion to kref compared to the original kref.Models trained with data transformation exhibited divergent edaphic‐climatic controls for different formatted kref.This study underscores the importance of directly focusing on original values rather than alternative representations. Plain Language Summary: The first‐order model is commonly used to simulate the dynamics of soil organic matter decomposition. The reference decomposition rates (kref) in the first‐order model are usually transformed into reciprocal or logarithmic formats, which may affect model performance as well as the regulatory patterns of edaphic‐climatic factors. In this study, we investigated the effects of data transformation on predictive performance and variable importance. We found that models trained with transformed kref exhibited reduced performance upon re‐conversion to kref, compared to those trained with the original kref. In addition, data transformation altered the distribution and range of the dependent variable, leading to divergent controlling roles of explanatory variables pertaining to models trained with different formatted kref. Our study underscores the need to apply models directly to the original variables rather than their transformed representations. [ABSTRACT FROM AUTHOR]

Li, W.-Y., 2019. Interaction mode of marine economic management talents cultivation and marine industry. In: Gong, D.; Zhu, H., and Liu, R. (eds.), Selected Topics in Coastal Research: Engineering, Industry, Economy, and Sustainable Development. Journal of Coastal Research, Special Issue No. 94, pp. 577–580. Coconut Creek (Florida), ISSN 0749-0208. In recent years, due to the rise of emerging marine industries and the state's support for marine technology, many universities in China have added specialties related to marine technology, focusing on cultivating marine technology talents. In contrast, the state does not pay enough attention to the training of marine economic management talents, making such marine talents scarce and the structure of marine talents unbalanced.This paper analyzes the current situation and reasons of the imbalance between supply and demand of marine economic management talents in China, and puts forward specific countermeasures and suggestions on how to develop the marine economy and give play to the subjective initiative of marine technology and marine economic management talents. [ABSTRACT FROM AUTHOR]

This paper reports that the in situ growth magnetic field (Hg) during magnetic-phase CoFeB deposition impacts the electric-field control of magnetic anisotropy in Co40Fe40B20/(011)-Pb(Mg1/3Nb2/3)0.7Ti0.3O3 [CoFeB/(011)-PMN-PT] composite multiferroic heterostructures at room temperature. In the Hg1 mode (in situ Hg along the [ 01 1 ¯ ] direction of the ferroelectric PMN-PT substrate), the electric-field-controlled modulation ratios of the magnetic coercivity HC and saturation magnetic field HS are approximately −47% and +156%, respectively. However, in the Hg2 mode (in situ Hg along the [100] direction of the ferroelectric PMN-PT substrate) of the CoFeB/(011)-PMN-PT multiferroic heterostructure, the electric-field-controlled modulation ratios of the magnetic coercivity HC and saturation magnetic field HS can reach as high as +162% and +393%, respectively. Moreover, the electric-field-controlled magnetic coercive field HC exhibits a butterfly shape when plotted versus the applied electric fields in both modes, which matches the in-plane butterfly strain loop of the ferroelectric PMN-PT substrate. However, the electric-field-controlled saturation magnetic field HS presents a square loop, which is very consistent with the ferroelectric loop of the PMN-PT substrate. This result may be ascribed to the distinct pathway of the ferroelastic domain switching in the (011)-oriented PMN-PT substrate. This study provides a new idea for the design of spintronic devices based on multiferroic heterostructures. [ABSTRACT FROM AUTHOR]

Glyceraldehyde-3-phosphate dehydrogenase-C (GapC) is a highly conserved surface protein of Staphylococcus aureus , with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity, which represents an excellent vaccine candidate antigen. It can induce protective immune responses to S. aureus infections. However, CD4+ T cell epitopes of GapC that induce CD4+ T cell immune responses are currently unclear. In this study, we used bioinformatics prediction algorithms to predict CD4+ T cell epitopes of GapC. Ten peptides were synthesized to investigate the candidate epitopes. Our results showed that the peptides, G4 (GapC 104-123) and G10 (GapC 314-333) were able to induce proliferation of CD4+ T cells and secrete high levels of interferon (IFN)-γ, respectively. In addition, they significantly reduced bacterial loads in tissue and induced immunoprotective effects. It is suggested that G4 and G10 are Th1-type epitopes of S. aureus GapC. This study provides the potential development of the design of epitope-based vaccine against S. aureus. • We identified two CD4+ T cell epitopes on GapC of S.aureus , which named G4 (GapC 104-123) and G10 (GapC 314-333). • G4 and G10 induced CD4+ T cell proliferation and secretion of IFN-γ, and reduced bacterial loads in tissues. • G4 and G10 are Th1 type CD4+ T cell epitopes and can induce a certain immune protection against S. aureus challenge. [ABSTRACT FROM AUTHOR]

We perform a CRISPR-Cas9 genome-wide screen in glioblastoma stem cells and identify integrin αvβ5 as an internalization factor for Zika virus (ZIKV). Expression of αvβ5 is correlated with ZIKV susceptibility in various cells and tropism in developing human cerebral cortex. A blocking antibody against integrin αvβ5, but not αvβ3, efficiently inhibits ZIKV infection. ZIKV binds to cells but fails to internalize when treated with integrin αvβ5-blocking antibody. αvβ5 directly binds to ZIKV virions and activates focal adhesion kinase, which is required for ZIKV infection. Finally, αvβ5 blocking antibody or two inhibitors, SB273005 and cilengitide, reduces ZIKV infection and alleviates ZIKV-induced pathology in human neural stem cells and in mouse brain. Altogether, our findings identify integrin αvβ5 as an internalization factor for ZIKV, providing a promising therapeutic target, as well as two drug candidates for prophylactic use or treatments for ZIKV infections. • CRISPR screens identify 92 GSC-specific factors linked to ZIKV infection • Integrin αvβ5 mediates ZIKV internalization into hNSCs providing two drug candidates • Integrin αvβ5 expression correlates with ZIKV susceptibility and neurotropism • Inhibition of αvβ5 alleviates ZIKV-induced pathology in hNSCs and in mouse brain Wang et al. show that Zika virus (ZIKV) uses integrin αvβ5 to infect neural stem cells. ZIKV infection can be inhibited by αvβ5 blocking antibody or inhibitors, SB273005 and cilengitide, in human neural stem cells and in mouse brain, providing drug candidates for prophylactic use or treatments for ZIKV infections. [ABSTRACT FROM AUTHOR]

Background: The global spread of mosquito-borne diseases (MBD) has presented increasing challenges to public health. The transmission of MBD is mainly attributable to the biting behaviors of female mosquitoes. However, the ecological pattern of hourly host-seeking behavior in Aedes albopictus and its association with climatic variables are still not well understood, especially for a precise requirement for establishing an effective risk prediction system of MBD transmission. Methods: Mosquito samples and data on mosquito hourly density and site-specific climatic variables, including temperature, relative humidity, illuminance and wind speed, were collected simultaneously in urban outdoor environments in Guangzhou during 2016–2018. Kernel regression models were used to assess the temporal patterns of hourly host-seeking behavior in mosquito populations, and negative binomial regression models in the Bayesian framework were used to investigate the associations of host-seeking behavior with climatic variables. Results: Aedes albopictus was abundant, constituting 82% (5569/6790) of the total collected mosquitoes. Host-seeking behavior in Ae. albopictus varied across time and was significantly influenced by climatic variables. The predicted hourly mosquito densities showed non-linear relationships with temperature and illuminance, whereas density increased with relative humidity but generally decreased with wind speed. The range of temperature estimates for female biting was 16.4–37.1 °C, peaking at 26.5 °C (95% credible interval: 25.3–28.1). During the favorable periods, biting behavior of female Ae. albopictus was estimated to occur frequently all day long, presenting a bimodal distribution with peaks within 2–3 h around both dawn and dusk (05:00–08:00 h and 16:00–19:00 h). Moreover, a short-term association in hourly density between the females and males was found. Conclusions: Our field-based modeling study reveals that hourly host-seeking behavior of Ae. albopictus exhibits a complex pattern, with hourly variation constrained significantly by climatic variables. These findings lay a foundation for improving MBD risk assessments as well as practical strategies for vector control. For instances of all-day-long frequent female biting during the favorable periods in Guangzhou, effective integrated mosquito control measures must be taken throughout the day and night. [ABSTRACT FROM AUTHOR]

Background: The rapid development of the telecommunications industry in the post-COVID-19 era has brought tremendous pressure to employees making them a high-risk group for job burnout. However, prior research paid less attention to the burnout of employees. Furthermore, social support and gender have separate effects on job burnout. This study explores the mechanism of stress perception on job burnout and examines the roles of social support and gender amid it. Method: This cross-sectional study was conducted from June 2023 to August 2023 in mainland China. A total of 39,507 were recruited by random sampling and online questionnaires, and 28,204 valid questionnaires were retained. SPSS (version 26.0) and PROCESS (Model 4 & 7) were used for correlation analysis, mediation analysis, and mediated moderation analysis. Result: Stress perception can positively predict the level of job burnout of employees in the telecommunications industry, and social support plays a partial mediating role, accounts for 8.01% of the total effect, gender moderates the first half of the path in this mediation model. At the same pressure level, female can perceive more social support than male. Conclusions: Under high pressure background, employees' job burnout varies depending on gender and the perception of social support. Therefore, telecommunications industry managers should adopt decompression measures and targeted social support resources for different groups. [ABSTRACT FROM AUTHOR]

The structural plane characteristic was the most critical factor for determining the self-stability ability of deep foundation pit vertical-rock-wall in layered rock stratum. Multiple methods such as model testing, numerical calculation, and theoretical calculation were utilized comprehensively in this paper. The self-stabilizing control effect on the deep foundation pits vertical-rock-wall that under the different structural plane inclination angle (α) and under the different structural plane strength was systematically studied. The results indicated that the overall variation trend of "Sharp decrease ~ Slow decrease ~ Slow increase ~ Sharp increase" in the symmetrical distribution for the self-stability critical height (Hcr) varied with the gradually increasing of α was presented. Meanwhile, the variation trend of "continuously decreasing and rapidly decreasing first, and then slowly decreasing and tending to stabilize" with the structural plane strength reduction coefficient (k). The key factor to control the self-stability of the deep foundation pit vertical-rock-walls lied in fully grasping and utilizing the basic characteristics of rock structural planes. The research results of this paper provided the theoretical basis for scientifically determining the safety level and designing reasonable support structures of the deep foundation pit vertical-rock-walls in layered rock stratum. [ABSTRACT FROM AUTHOR]

To address the need for multivalent vaccines against Coronaviridae that can be rapidly developed and manufactured, we compared antibody responses against SARS-CoV, SARS-CoV-2, and several variants of concern in mice immunized with mRNA-lipid nanoparticle vaccines encoding homodimers or heterodimers of SARS-CoV/SARS-CoV-2 receptor-binding domains. All vaccine constructs induced robust anti-RBD antibody responses, and the heterodimeric vaccine elicited an IgG response capable of cross-neutralizing SARS-CoV, SARS-CoV-2 Wuhan-Hu-1, B.1.351 (beta), and B.1.617.2 (delta) variants. [ABSTRACT FROM AUTHOR]

Tetrabromobisphenol A (TBBPA) has attracted a great deal of attention due to its side effects and potential bioaccumulation properties. It is of great importance to construct and develop novel electrochemical sensors for the sensitive and selective detection of TBBPA. In the present study, cobalt (Co) based metal–organic frameworks (MOFs) were synthesized on carbon cloth (CC) by using cobalt nitrate hexahydrate and 2-methylimidazole. The morphological characterization was carried out by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). The results showed that Co-MOFs/CC have a leaf-like structure and abundant surface functional groups. The electrochemical properties of the sensor were investigated by differential pulse voltammetry (DPV). The effects of different ratios of metal ions to organic ligands, reaction temperature, time, concentration, pH value of the electrolyte, and incubation time on the oxidation peak current of TBBPA were studied. Under the optimal conditions, the linear range of the designed sensor was 0.1 μM–100 μM, and the limit of detection was 40 nM. The proposed sensor is simple, of low cost and efficient, which can greatly facilitate the detection tasks of environmental monitoring workers. [ABSTRACT FROM AUTHOR]

The main objective of this study is to evaluate the influence of exosomes derived from endothelial progenitor cells (EPC-Exo) on neointimal formation induced by balloon injury in rats. Furthermore, the study aims to investigate the potential of EPC-Exo to promote proliferation, migration, and anti-apoptotic effects of vascular endothelial cells (VECs) in vitro. The underlying mechanisms responsible for these observed effects will also be thoroughly explored and analyzed. Endothelial progenitor cells (EPCs) was isolated aseptically from Sprague–Dawley (SD) rats and cultured in complete medium. The cells were then identified using immunofluorescence and flow cytometry. The EPC-Exo were isolated and confirmed the identities by western-blot, transmission electron microscope, and nanoparticle analysis. The effects of EPC-Exo on the rat carotid artery balloon injury (BI) were detected by hematoxylin and eosin (H&E) staining, ELISA, immunohistochemistry, immunofluorescence, western-blot and qPCR. LPS was used to establish an oxidative damage model of VECs. The mechanism of EPC-Exo repairing injured vascular endothelial cells was detected by measuring the proliferation, migration, and tube function of VECs, actin cytoskeleton staining, TUNEL staining, immunofluorescence, western-blot and qPCR. In vivo, EPC-Exo exhibit inhibitory effects on neointima formation following carotid artery injury and reduce the levels of inflammatory factors, including TNF-α and IL-6. Additionally, EPC-Exo downregulate the expression of adhesion molecules on the injured vascular wall. Notably, EPC-Exo can adhere to the injured vascular area, promoting enhanced endothelial function and inhibiting vascular endothelial hyperplasia Moreover, they regulate the expression of proteins and genes associated with apoptosis, including B-cell lymphoma-2 (Bcl2), Bcl2-associated x (Bax), and Caspase-3. In vitro, experiments further confirmed that EPC-Exo treatment significantly enhances the proliferation, migration, and tube formation of VECs. Furthermore, EPC-Exo effectively attenuate lipopolysaccharides (LPS)-induced apoptosis of VECs and regulate the Bcl2/Bax/Caspase-3 signaling pathway. This study demonstrates that exosomes derived from EPCs have the ability to inhibit excessive carotid intimal hyperplasia after BI, promote the repair of endothelial cells in the area of intimal injury, and enhance endothelial function. The underlying mechanism involves the suppression of inflammation and anti-apoptotic effects. The fundamental mechanism for this anti-apoptotic effect involves the regulation of the Bcl2/Bax/Caspase-3 signaling pathway. [ABSTRACT FROM AUTHOR]

Background: The most serious challenges in medicinal 'Sanghuang' mushroom production are the fungal diseases caused by various molds. Application of biological agents has been regarded as a potential crop disease management strategy. Here, the soil microbiome associated with 'Sanghuang' mushroom affected by fungal diseases grown under field cultivation (FC) and hanging cultivation (HC) was characterized using culture-dependent and culture-independent methods. Results: A total of 12,525 operational taxonomic units (OTUs) and 168 pure cultures were obtained using high-throughput sequencing and a culture-dependent method, respectively. From high-throughput sequencing, we found that HC samples had more OTUs, higher α-diversity, and greater microbial community complexity than FC samples. Analysis of β-diversity divided the soil microbes into two groups according to cultivation mode. Basidiomycota (48.6%) and Ascomycota (46.5%) were the two dominant fungal phyla in FC samples, with the representative genera Trichoderma (56.3%), Coprinellus (29.4%) and Discosia (4.8%), while only the phylum Ascomycota (84.5%) was predominant in HC samples, with the representative genera Discosia (34.0%), Trichoderma (30.2%), Penicillium (14.9%), and Aspergillus (7.8%). Notably, Trichoderma was predominant in both the culture-independent and culture-dependent analyses, with Trichoderma sp. FZ0005 showing high host pathogenicity. Among the 87 culturable bacteria, 15 exhibited varying extents of antifungal activity against Trichoderma sp. FZ0005, with three strains of Bacillus spp. (HX0037, HX0016, and HX0039) showing outstanding antifungal capacity. Conclusions: Overall, our results suggest that Trichoderma is the major causal agent of 'Sanghuang' fungal diseases and that Bacillus strains may be used as biocontrol agents in 'Sanghuang' cultivation. [ABSTRACT FROM AUTHOR]

Soil respiration (Rs) during daytime (Rsday) and nighttime (Rsnight) remains poorly understood owing to the limited availability of high‐resolution temporal measurements of Rs.Using continuous automatic chamber measurements at a half‐hourly interval, we explored Rs and its temperature sensitivity (Q10) during the daytime and nighttime over 10 years and determined the best sampling timeslot in a temperate deciduous broadleaf forest.We demonstrate a higher Rsnight than Rsday in spring and summer, a lower Rsnight than Rsday in autumn, but an insignificant difference between them in winter, contrasting with the consensus of a higher Rsday than Rsnight. Such discrepancy may result from the effect of factors other than temperature (e.g. reallocation of photosynthesis carbon, precipitation and soil moisture). We also reveal significant differences in apparent Q10 between seasons, indicating the highest Q10 in spring and significantly higher Q10 at night than at day in autumn. The Rs measured around midday (9:00–11:00 for autumn and 12:00–15:00 for other seasons) and midnight (20:00–22:00 for autumn and 0:00–2:00 for other seasons) provides the lowest bias to the daily mean Rs, whereas the measurement timeslots around midday are preferred due to practical considerations.Our findings highlight the significant diel variation of Rs and its temperature sensitivity across seasons and the necessity to account for Rsnight for a more accurate estimate of soil carbon budget. Read the free Plain Language Summary for this article on the Journal blog. [ABSTRACT FROM AUTHOR]

Background: Hepatoid adenocarcinoma of the stomach (HAS) is a highly malignant and rare extrahepatic tumor. The prognosis is controversial because of its rarity and the lack of multi-center cohort studies, especially on the influence of serum Alpha-fetoprotein (AFP) level on prognosis. We aimed to analyze the clinicopathological characteristics and prognosis of HAS, particularly the effect of serum AFP on the prognosis of HAS. Methods: We retrospectively reviewed clinical data of one HAS patient treated at our institution in 2019 and of 252 patients reported between 1984 and 2020 in research databases. Results: Among these patients, 60.1% were > 60 years, 51% had lesions in the gastric antrum, and 51.0% (73/143) had the ulcerative lesion type. The preoperative elevated levels of serum alpha-fetoprotein (AFP) were detected in most patients (76.7%). Lymph-node (84.6%) and preoperative liver metastasis (39.1%) were often found. The high-AFP group was characterized by a higher rate of stage IV (P = 0.000682) and liver metastasis (P = 0.000068). The 1-, 3-and 5-year progression-free survival(PFS) rates were 41%, 18%, and 0%, and the 1-, 3-, and 5-year overall survival (OS) rates were 64%, 26%, and 21%, respectively. The survival analysis showed that OS was significantly shorter for HAS with high-AFP (> 300 ng/ml) than with low-AFP (≤ 300 ng/ml) (P = 0.023). The univariate analysis indicated that the OS of HAS was associated with tumor location, pTNM stage, lymph-node metastasis, surgical resection, and serum AFP > 300 ng/ml. However,the prognostic factors for PFS was only pTNM stage and surgical resection. The multivariate analysis confirmed that the independent prognostic factor affecting OS of HAS included pTNM stage and surgical resection. Conclusions: Liver metastasis was increasingly more likely with increasingly higher serum AFP, but the prognosis of HAS is not necessarily poor. Serum AFP level is an important prognostic indicator in HAS and should be monitored. [ABSTRACT FROM AUTHOR]

Adenosine N6‐methylation (m6A) and N6,2′‐O‐dimethylation (m6Am) are regulatory modifications of eukaryotic mRNAs. m6Am formation is catalyzed by the methyl transferase phosphorylated CTD‐interacting factor 1 (PCIF1); however, the pathophysiological functions of this RNA modification and PCIF1 in cancers are unclear. Here, we show that PCIF1 expression is upregulated in colorectal cancer (CRC) and negatively correlates with patient survival. CRISPR/Cas9‐mediated depletion of PCIF1 in human CRC cells leads to loss of cell migration, invasion, and colony formation in vitro and loss of tumor growth in athymic mice. Pcif1 knockout in murine CRC cells inhibits tumor growth in immunocompetent mice and enhances the effects of anti‐PD‐1 antibody treatment by decreasing intratumoral TGF‐β levels and increasing intratumoral IFN‐γ, TNF‐α levels, and tumor‐infiltrating natural killer cells. We further show that PCIF1 modulates CRC growth and response to anti‐PD‐1 in a context‐dependent mechanism with PCIF1 directly targeting FOS, IFITM3, and STAT1 via m6Am modifications. PCIF1 stabilizes FOS mRNA, which in turn leads to FOS‐dependent TGF‐β regulation and tumor growth. While during immunotherapy, Pcif1‐Fos‐TGF‐β, as well as Pcif1‐Stat1/Ifitm3‐IFN‐γ axes, contributes to the resistance of anti‐PD‐1 therapy. Collectively, our findings reveal a role of PCIF1 in promoting CRC tumorigenesis and resistance to anti‐PD‐1 therapy, supporting that the combination of PCIF1 inhibition with anti‐PD‐1 treatment is a potential therapeutic strategy to enhance CRC response to immunotherapy. Finally, we developed a lipid nanoparticles (LNPs) and chemically modified small interfering RNAs (CMsiRNAs)‐based strategy to silence PCIF1 in vivo and found that this treatment significantly reduced tumor growth in mice. Our results therefore provide a proof‐of‐concept for tumor growth suppression using LNP‐CMsiRNA to silence target genes in cancer. Synopsis: m6Am formation in eukaryotic mRNAs is catalyzed by PCIF1, a cap‐specific adenine N6‐methyltransferase; however, the function and mechanism of m6Am modification and PCIF1 in colorectal cancer remain unclear. Here, we reveal that PCIF1 modulates colorectal cancer malignancy and response to anti‐PD‐1 immunotherapy in a context‐dependent manner. PCIF1 is overexpressed in colorectal cancer (CRC) and its increased expression negatively correlates with patient survival.Disruption of PCIF1 inhibits tumor malignancy and sensitizes colorectal cancer to immunotherapy in a murine model.FOS, IFITM3, and STAT1 are targets regulated by PCIF1‐mediated m6Am modification.Pcif1 depletion in tumors enhances the number of tumor‐infiltrating NK cells and modulates TGF‐β, IFN‐γ and TNF‐α secretion during anti‐PD1 treatment.Lipid‐nanoparticles (LNPs) and chemically modified small interfering RNAs (CMsiRNAs) mediated silencing of PCIF1 in vivo significantly reduces tumor growth in mice. [ABSTRACT FROM AUTHOR]

A novel method was developed for the synergetic accumulation and simultaneous determination of naphthol isomers (α-N and β-N) at electrochemically reduced graphene oxide modified glassy carbon electrode (E-rGO/GCE). One-step electrochemical reduction was employed for the fabrication of E-rGO/GCE, then the modified material was characterized by scanning electron microscope, Raman spectroscopy and Fourier transform infrared spectroscopy. Electrochemical behavior of naphthol isomers at E-rGO/GCE shows that the sensitivity of the isomers could be increased due to the synergistic effect of π–π conjugating and electrochemical accumulation. The oxidation peaks of α- and β-naphthol could be completely separated and the isomers were simultaneously detected with the linear ranges of 5–400 nM and 0.5–20 μM for α-N, 5–350 nM and 0.5–20 μM for β-N. The detection limits of α-N and β-N were 1.01 nM and 0.43 nM (S/N = 3), and the corresponding sensitivity were 0.0097 μA/μM and 0.0113 μA/μM, respectively. More importantly, the naphthol isomers electrochemical sensor on E-rGO film with synergistic effect can be expected to result in significant improvements in the sensor performance and the developed method has been successfully applied to human urine sample with a satisfactory result. [ABSTRACT FROM AUTHOR]

[Display omitted] • River algal blooms are complicated and difficult to forecast. • Convergent Crossing-Mapping detects causality for river algal blooms. • Machine learning models exhibit robust performance in predicting chlorophyll-a. River algal blooms pose a global ecological and environmental problem, resulting in serious consequences for watershed ecosystems and human health. However, current research has yet to fully identify factors influencing river algal blooms or accurately predict chlorophyll-a concentration, a key indicator, at various lead-times using high-frequency data. Here, we employed Empirical Dynamic Modeling (EDM) and machine learning techniques to forecast daily chlorophyll-a concentration in the Han River of China. The Convergent Cross-Mapping (CCM) analysis revealed the causal relationships between chlorophyll-a concentration and eight variables: pH, total nitrogen, water level in the Han River, water level in the Yangtze River, water temperature, permanganate index, sunlight duration and total phosphorus in the Han River. Subsequent multivariate EDM models with three lead-times (i.e., 1-day, 5-day, and 10-day) only showed acceptable performance in model training (R2 = 0.19–0.73, MAE = 2.76–9.17 μg/L), while exhibited poor predictive performance in model testing (R2 = − 0.50–0, MAE = 9.64–13.09 μg/L). However, Gradient Boosting Machine (GBM) and Random Forest (RF) models with three lead-times exhibited robust performance in both model training (R2 ≥ 0.8, MAE < 4 μg/L) and testing (R2 > 0.6, MAE < 6 μg/L), demonstrating that machine learning models were more suitable than multivariate EDM models for reliably predicting algal blooms. Our study contributes valuable tools for predicting daily chlorophyll-a concentration. The methods presented herein hold broad applicability and offer insights into predicting the entire process of river algal blooms based on daily monitoring data. [ABSTRACT FROM AUTHOR]

[Display omitted] • Novel TDES was prepared to realize the double donor grafting of lignin. • Regenerated lignin had low condensation structure and high hydroxyl content. • The correlation between lignin structure and fluorescence properties was constructed. • Lignin-Lum-MDI with high fluorescence intensity was applied to information encryption. The high-valued utilization of biomass components through pretreatment technology can effectively alleviate the pressure of energy and environment. In this study, a ternary deep eutectic solvent (TDES) was prepared by using glycolic acid (GA) and ethylene glycol (EG) as double hydrogen donors and benzyl trimethyl ammonium chloride (TMBAC) as hydrogen acceptor. The corn stalk was separated by TDES to obtain cellulose-rich solid residue and low-condensed lignin. Moreover, the solid residue was converted into bio-oil by rapid pyrolysis, and the low-condensed lignin was converted into fluorescent lignin by grafting luminol for information encryption. The results showed that under the pretreatment condition of 150℃ for 3 h, TDES with the molar ratio of 1:1:2 had a lignin removal ratio of 90.60 %, and the yield of levoglucosan obtained from solid residue pyrolysis bio-oil reached 36.38 %. Furthermore, TDES prevented lignin from forming a C-C polycondensation structure and endowed it with polyhydroxy properties, which was beneficial to graft luminol to enhance the fluorescence intensity efficiently. The high-intensity fluorescent grafted lignin was used for information encryption of various materials. The mechanism of TDES pretreatment and luminol grafting were explored, and the mass balance of corn stalk during pretreatment was described. This study provides a green, environmentally friendly, and efficient pretreatment process for the high-value utilization of biomass. [ABSTRACT FROM AUTHOR]

Saccharomyces boulardii (Sb) is a probiotic yeast for the treatment of gastrointestinal disorders, including inflammatory bowel disease (IBD). Little is known about the modulatory capacity of the Sb in IBD. Here, we found that oral gavage of Sb supernatant (SbS) alleviated gut inflammation, protected the intestinal barrier, and reversed DSS-induced down-regulated activation of epidermal growth factor receptor (EGFR) in colitis. Mass spectrum analysis showed that thioredoxin (Trx) is one of the critical secreted soluble proteins participating in EGFR activation detected in SbS. Trx exerted an array of significant effects on anti-inflammatory activity, including alleviating inflammation, protecting gut barrier, suppressing apoptosis, as well as reducing oxidative stress. Mechanistically, Trx promoted EGFR ligand gene expression and transactivated EGFR in a concentration-dependent manner. EGFR kinase inhibitor could block Trx-mediated preventive effects of intestinal epithelial injury. Our data suggested that Sb -derived soluble protein Trx could serve as a potential prophylactic, as a novel postbiotic against colitis, which provides a new strategy for the precision prevention and treatment of IBD. [Display omitted] • Oral gavage of SbS alleviated gut inflammation and protected the intestinal barrier in DSS-induced colitis. • Trx, a soluble protein derived from Sb , could transactivate the EGFR/Akt pathway in a concentration-dependent manner. • Trx could suppress intestinal inflammation, protect the gut barrier and lessen oxidative injury to alleviate colitis. • Trx may be a novel prophylactic as a postbiotic for the therapy of colitis. [ABSTRACT FROM AUTHOR]

[Display omitted] • Organic solvent-assisted EDA pretreatment separated 96% lignin and grafted with –NH 2. • One-pot pretreatment realized integrated preparation of fluorescent L-CPD and H-CPD. • The separated components were used in pyrolysis saccharification, information encryption and Fe3+ detection respectively. • Interaction and reaction sites between solvent and lignin were calculated by DFT. In order to achieve the goal of carbon neutrality, it is an important way and a great challenge to realize the full-component conversion of lignocellulosic biomass via bio-refinery. In this study, the pretreatment method of organic solvent-assisted ethylenediamine was used to separate cellulose, hemicellulose, and lignin in corn stalk. The effects of different organic solvents on the separation ability were studied, and the separated products were transformed into high-value products. Specifically, the structures of solid residue, regenerated hemicellulose, and regenerated lignin were analyzed and applied to pyrolysis saccharification (at 500℃ for 30 min), information anti-counterfeiting, and selective detection of Fe3+ respectively. The results showed that the lignin removal ratio of 1-butyl-3-lethylimidazolyl chloride/ethylenediamine (IL/EDA) system could reach 96.05 % under the pretreatment condition of 200℃ for 2 h, and the yield of Levoglucosan obtained from pyrolysis bio-oil reached 169.70 mg·g−1 with high selectivity. Moreover, IL/EDA pretreatment increased the nitrogen content of lignin and hemicellulose, enhanced the fluorescence intensity, and effectively realized selective detection and information encryption. Furthermore, Density functional theory (DFT) calculation proved that the addition of organic solvents was beneficial to the reaction between lignin and ethylenediamine (EDA). The Fukui function was used to explore the reaction site between EDA and lignin. In addition, the mass balance of the pretreatment process was also described. This study realized the high-value application of all components of lignocellulose and provided a new process for the efficient utilization of green carbon resources. [ABSTRACT FROM AUTHOR]

[Display omitted] • Coupling pretreatment of DDQ pre-oxidation and HCOOH extraction for SCB. • Yield of lignin monomer increased by nearly 2 times via inhibiting condensation. • Tensile strength of LCS increased by nearly 2 times. • Mechanism of coupling effects of pre-oxidation and extraction was investigated. The full utilization of biomass plays an important role in the environment and energy. In this study, the coupling pretreatment of DDQ pre-oxidation and HCOOH extraction was employed to realize the separation and modification of three components of sugarcane bagasse for full utilization. Especially, the modified lignin was depolymerized to obtain aromatic monomers, and cellulose-rich residue was used to prepare lignocellulose straws. Furthermore, the coupling effects between DDQ pre-oxidation and HCOOH extraction on chemical and physical properties of modified lignin and lignocellulose straws were studied. In addition, the mechanism of lignin depolymerization and lignocellulose straw synthesis were explored as well as the mass balance of the biorefinery process. The results showed that the total yield of aromatic monomers increased by nearly 2 times and the conversion of lignin was 95.1 % after DDQ pre-oxidation. The mechanism showed that DDQ selectively oxidized Cα-OH bond of lignin to Cα = O bond, which not only avoided the formation of carbocation, but also reduced the bond dissociation energy of β-O-4 bond. Moreover, the tensile strength of hydrophobic lignocellulose straw increased by nearly 2 times and its flexural strength was 10.36 MPa. Modified lignin and modified cellulose were connected via hydrogen bonds, which enhanced the intermolecular crosslinking of molecules, thus improving the mechanical properties of lignocellulose straws. Besides, the maximum xylose relative content and concentration of xylose were 61.16 % and 17.57 g/L, respectively. This study provides a method for high-value and green utilization of sugarcane bagasse. [ABSTRACT FROM AUTHOR]

• The Multi-FVC model was developed based on PROSAIL model and Beer-Lambert law to retrieve FVC. • Multi-FVC model was more potential in estimating FVC and suppressing the influence of soil. • Soil effect caused by the second axis in the soil spectral line could be solved by NDVI and EVI. • Multi-FVC model could realize the conversion of FVC retrieval results at any two view zenith angles. Fractional vegetation cover (FVC) plays an important role in spectral unmixing, crop growth monitoring, crop light interception calculation, and yield estimation. However, the spectral reflectance would change with view zenith angles (VZAs), and retrieved FVC is also affected by VZAs. Therefore, in this paper, the observed multi-angular (±45°, ±30°, 0°) spectral datasets with different crops (wheat and rice), along with simulated spectral dataset, were used to explore method of correcting the influence of angle effect in FVC retrieval. Firstly, a simulated dataset of multi-angular hyperspectral data and directional FVC were constructed using the PROSAIL model, and a single-angular FVC retrieval model (Sin-FVC) was established based on the Gaussian process regression (GPR) algorithm. Then, the single-angular FVC (i.e., FVC θ) was converted into vertical FVC (FVC θv) based on the Beer-Lambert law. Finally, a Multi-FVC model was developed, which summed outputs of the Sin-FVC model (FVC 0° , FVC θv) according to their respective weights (i.e., FVC correct), to correct the angle effect. Using the pixel bisection model (FVC VI) as a comparison, different FVC retrieval models (Sin-FVC, Multi-FVC, and FVC VI) were compared and evaluated in terms of FVC retrieval accuracy, ability in weakening soil background, and LNC retrieval accuracy after spectral decomposition using different retrieved FVC (FVC 0° , FVC -30°v , FVC -45°v , FVC correct). The results showed that FVC correct had the highest FVC retrieval accuracy (RRMSE = 8.5 %) compared with FVC retrieved from other models (FVC VI , FVC 0° , FVC -30°v and FVC -45°v). Meanwhile, when using the reflectance at NIR band in the black soil background as the baseline, after decoupling mixing spectra based on FVC correct , the relative offset (i.e., RO correct) of each vegetation index (NDVI, EVI, SAVI, OSAVI) was minimum (least-RO correct = 0.42 %). That is, the soil background was effectively suppressed by FVC correct spectral decomposition, along with the highest LNC retrieval accuracy, with an RRMSE of 14.2 %. [ABSTRACT FROM AUTHOR]

Background and aims: Hepatitis B surface antigen (HBsAg) seroconversion is considered the optimal outcome of the treatment of chronic hepatitis B virus (HBV) infection. In this study, we aimed to determine the cellular and molecular mechanisms by which pegylated interferon alpha (PEG-IFN-α) improves the seroconversion rate in patients with chronic hepatitis B (CHB). Methods: Flow cytometry was performed using circulating T follicular helper (TFH) cells from 15 healthy individuals and 45 patients with CHB presenting different treatment responses [complete response group (CRG), incomplete response group (ICRG), and nonresponse group (NRG)] to the standard 48-week regimen of PEG-IFN-α monotherapy to examine the significance of circulating TFH cells in the therapeutic response of patients with CHB to PEG-IFN-α. In addition, the capacities of different TFH subsets to activate B cells and stimulate IgG production were assessed by performing coculture experiments. Results: Longitudinal analysis revealed specific and significant increases in the numbers of CD40L+CD4+CXCR5+ TFH cells in the CRG compared with the NRG and ICRG. According to the results of in vitro coculture experiments, blocking CD40-CD40L signaling, but not ICOS–ICOSL signaling, specifically inhibits B-cell activation and IgG production. HBV may impair TFH cell function by enhancing inhibitory regulatory T-cell activity. Transcriptome analysis further revealed the upregulation of CD40L, but not of ICOS, in TFH cells isolated from the CRG. Conclusions: TFH cells, particularly those with CD40L expression, stimulate B-cell differentiation and improve the HBsAg seroconversion rate in patients with CHB treated with PEG-IFN-α monotherapy. [ABSTRACT FROM AUTHOR]

Guhan Yangsheng Jing (GHYSJ) is a traditional Chinese patent medicine, that has the function of nourishing the kidney and replenishing the essence, invigorating the brain and calming the mind. It is often used to treat dizziness, memory loss, sleep disorders, fatigue, and weakness, etc. However, its mechanism for improving sleep has not yet been determined. This study aims to explore the effects of GHYSJ on Sleep Deprivation (SD)-induced hippocampal neuronal pyroptotic injury, learning and cognitive abilities, and sleep quality in mice. In this study, a PCPA-induced SD mouse model was established. We assessed the influence of GHYSJ on sleep quality and mood by using the pentobarbital-induced sleep test (PIST) and sucrose preference test (SPT). The pharmacological effects of GHYSJ on learning and memory impairment were evaluated by the Morris Water Maze (MWM) and Open Field Test (OFT). Pathological changes in the hippocampal tissue of the SD rats were observed via HE staining and Nissl staining. The severity of neuronal damage was evaluated by detecting the expression of the neuronal marker Microtubule-associated protein 2 (MAP2), via immunohistochemistry and immunofluorescence. Furthermore, the levels of neurotransmitter 5-hydroxytryptophan (5-HTP), 5-hydroxy tryptamine (5-HT), γ-aminobutyric acid (GABA), and Glutamic acid (Glu) in hippocampal tissues, as well as the expression of inflammatory factors Interleukin-1β (IL-1β) and Interleukin-18 (IL-18) in serum, were determined by ELISA. The expressions of mRNA and protein NOD-like receptor thermal protein domain associated protein 3 (NLRP3), Gasdermin D (GSDMD), Cysteinyl aspartate specific proteinase1 (Caspase1), High mobility group box-1 protein (HMGB1) and Apoptosis-associated speck-like protein containing CARD (ASC) related to the cellular ferroptosis pathway were tested and analyzed by RT-PCR and WB respectively. PCPA significantly diminishes the sleep span of experimental animals by expediting the expenditure of 5-HT, consequently establishing an essentially direct SD model. The intervention of GHYSJ displays remarkable efficacy in mitigating insomnia symptoms, encompassing difficulties in initiating sleep and insufficient sleep duration. Likewise, it ameliorates memory function impairments induced by sleep deprivation, along with symptoms such as fatigue and depletion of vitality. GHYSJ exerts a protective influence on hippocampal neurons facilitated by inhibiting the down regulation of MAP2 and maintaining the equilibrium of neurotransmitters (5-HTP, 5-HT, GABA, and Glu). It diminishes the expression of intracellular pyroptosis-associated inflammatory factors (IL-1β and IL-18) and curbs the activation of the NLRP3/Caspase1/GSDMD pyroptosis-related signaling pathways, thereby alleviating the damage caused by hippocampal neuronal pyroptosis. [Display omitted] • GHYSJ intervention can effectively reduce symptoms of insomnia, including difficulty falling asleep and insufficient sleep duration. • GHYSJ improves symptoms caused by sleep deprivation, such as decline in learning and memory functions, fatigue, and loss of vitality. • GHYSJ has the effect of alleviating pyroptosis-induced damage to hippocampal neurons. By inhibiting the reduction of MAP2 and regulating neurotransmitter balance ， it reduces the expression of inflammation factors related to cellular pyroptosis, and plays a role by inhibiting the activation of the NLRP3/Caspase1/GSDMD pyroptosis-related signaling pathway. [ABSTRACT FROM AUTHOR]

• Kupffer cell-specific Cre-driven RiboTag reporter allows translatome analysis. • Fetal-derived Kupffer cells are relatively radioresistant. • Adult monocyte-derived Kupffer cells are sensitive to lethal irradiation. • p21Cip/WAF1 regulates the radioresistance of the fetal subset. Cells of hematopoietic origin, including macrophages, are generally radiation sensitive, but a subset of Kupffer cells (KCs) is relatively radioresistant. Here, we focused on the identity of the radioresistant KCs in unmanipulated mice and the mechanism of radioresistance. We employed Emr1- and inducible CX3Cr1-based fate-mapping strategies combined with the RiboTag reporter to identify the total KCs and the embryo-derived KCs, respectively. The KC compartment was reconstituted with adult bone-marrow-derived KCs (bm-KCs) using clodronate depletion. Mice were lethally irradiated and transplanted with donor bone marrow, and the radioresistance of bone-marrow- or embryo-derived KCs was studied. Gene expression was analyzed using in situ mRNA isolation via RiboTag reporter mice, and the translatomes were compared among subsets. Here, we identified the radioresistant KCs as the long-lived subset that is derived from CX3CR1-expressing progenitor cells in fetal life, while adult bm-KCs do not resist irradiation. While both subsets upregulated the Cdkn1a gene, encoding p21-cip1/WAF1 protein, radioresistant embryo-derived KCs showed a greater increase in response to irradiation. In the absence of this molecule, the radioresistance of KCs was compromised. Replacement KCs, derived from adult hematopoietic stem cells, differed from radioresistant KCs in their expression of genes related to immunity and phagocytosis. Here, we show that, in the murine liver, a subset of KCs of embryonic origin resists lethal irradiation through Cdkn1a upregulation and is maintained for a long period, while bm-KCs do not survive lethal irradiation. Kupffer cells (KCs) are the tissue-resident macrophages of the liver. KCs can be originated from fetal precursors and from monocytes during the fetal stage and post-birth, respectively. Most immune cells in mice are sensitive to lethal-irradiation-induced death, while a subset of KCs resists radiation-induced death. These radioresistant KCs continue to live in the irradiated mice. We discovered that this relatively radioresistant KC subset are the fetal-derived KCs, and they achieve this through cell-cycle arrest. Understanding the radiobiology of KCs will provide valuable insights into the mechanisms that elicit radiation-induced liver disease. [ABSTRACT FROM AUTHOR]

• In this paper, a new composite phase change material with date nucleus occupation structure based on Na 2 CO 3 -K 2 CO 3 /SiO 2 phase change material was proposed and designed. • The thermal transport properties of a new composite material from 600 to 1300 K were studied by molecular dynamics. • The heat transport prediction formula and method with a correlation coefficient of up to 0.99 were obtained. • In-depth analysis of the microscopic mechanisms of heat transport properties from a nanoscale energy perspective. Carbonate molten salts play a crucial role in energy transmission and storage for concentrated solar power (CSP) plants. In this study, a design strategy employing a date nucleus occupation structure was proposed for carbonate molten salt/ceramic SiO 2 composite phase change materials (PCMs). Molecular dynamics (MD) simulations were conducted to investigate the thermal transport properties and nanoscale atomic structure of the Nanocomposite phase change materials (NPCM) system. The findings revealed that the incorporation of SiO 2 nanoparticles significantly reduced the melting point of NPCM system, offering a favorable way to regulate the operating temperature range of the system. Moreover, the thermal conductivity and specific heat capacity of NPCM system increased with the increase in the radii of nanoparticles, reaching up to 12.32% and 6.061%, respectively. However, it was observed that larger nanoparticles limited the migration of particles, leading to a decrease in the self-diffusion coefficient (D) of the system. This study provides valuable insights in the design and application of CSP power plants utilizing nano-phase change materials. [ABSTRACT FROM AUTHOR]

• Three novel SDES were prepared for corn stalks pretreament. • The yield of carbohydrates in pyrolysis oil increased from 0.63% to 49.37%. • Multi-purpose fluorescent lignin was prepared from regenerated lignin. • Weak interaction and frontier orbital between molecules were calculated by DFT. In this study, β -CD was used as a receptor to prepare three novel SDES, which were used to pretreat corn stalks for obtaining fluorescent lignin and promoting biomass pyrolytic saccharification. It was found that GA-residue had a high cellulose retention ratio (94.63%) and the highest lignin removal ratio (61.78%). Besides, the yield of carbohydrates in bio-oil was increased from 0.63% to 49.37%, and fluorescent lignin was prepared for explosion detection, fluorescent film, and information encryption. It was confirmed that the weak interaction between β -CD and HBDs or dimer was mainly performed by hydrogen bond and van der Waals force. The minimum frontier orbital energy difference ΔE U (0.1976 a.u.) and high binding energy (-5456.71 kJ/mol) between molecules were calculated by DFT. Moreover, the mechanism of biomass pretreatment was explored. The green and efficient SDES developed in this study were of great significance for biomass pretreatment and efficient utilization of components. [ABSTRACT FROM AUTHOR]

Colloids may play an important role in the geochemical cycle of antimony (Sb). However, the controlling behaviors of colloids on Sb fate in contaminated groundwater are not available. To investigate the effects of colloids on Sb mobility, groundwater samples from Xikuangshan Sb Mine's two main aquifers (the D 3 s2 aquifer and the D 3 x4 aquifer) were successively (ultra)filtered through progressively decreasing pore sizes (0.45 µm, 100 kDa, 50 kDa and 5 kDa). The results showed that 0.1–84.1% of Sb was adsorbed or carried by colloids, which corresponded to Sb concentration ranging between 0 and 2973 μg/L in the colloids (0.45 µm - 5 kDa). In both aquifers, Sb was closely associated with organic colloids (r = 0.72 p < 0.05 for the D 3 x4 aquifer, r = 0.94 p < 0.01 for the D 3 s2 aquifer). Parallel factor analysis of the three-dimensional fluorescence spectra determined that the protein-like substances in the D 3 x4 aquifer and the humus-like substances in the D 3 s2 aquifer controlled Sb behavior. X-ray absorption spectroscopy confirmed Sb complexing with organic substances. Competitive adsorption of As and Sb suppressed the complexation of colloids with Sb, particularly in the D 3 x4 aquifer (r = –0.71, p < 0.05). Sb mobility was also influenced by the redox of the groundwater system. As the oxidation-reduction potential and dissolved oxygen increased, Sb in the colloidal fractions decreased. These findings provide new insights into the mechanisms involved in Sb fate affected by colloids, establishing the theoretical basis for developing effective Sb and even metalloid pollution remediation strategies. [Display omitted] • Colloids are important in Sb geochemical cycling. • Colloidal Sb behavior was mainly controlled by organic colloids. • Arsenic competes for Sb adsorption sites on colloids. • Oxidizing conditions reduced the proportion of Sb in colloids. [ABSTRACT FROM AUTHOR]

A comprehensive demonstration of Zika virus (ZIKV) molecular evolution is essential for understanding its adaptation and expansion in its recent pandemics. Despite several studies on mutations and codon usage in ZIKVs, the variations in codon usage patterns across individual genes and their biological implication remains unclear. Here, we performed a gene-by-gene comparison of the codon usage variation in ZIKVs of the African and Asian lineages. We found that besides the evidence of positive selection (Ka/Ks >1) in the Asian lineage of the ZIKV genome, codon usage patterns were gene-specific and codon usage variation of ZIKV genes, was possibly constrained by their individual functional features, such as transmembrane domains, or antigenicity. In particular, the NS2B and NS4A genes showed distinct codon usage patterns, clearly separating them from the clusters of other genes in the correspondence analysis (CA). In the Asian lineage, the NS2B and NS4A genes showed the highest codon usage bias (ENC values: 51.01 ± 0.72 and 48.89 ± 0.99 respectively), and were subjected to the highest translation selection (ENC obs /ENC exp ratio: 0.847 ± 0.0297 and 0.828 ± 0.0233 respectively) in comparison to the African lineages of ZIKV. The CpG frequency of the NS2B showed a gradual ascending trend in the Asian ZIKV lineages, while in NS4A it was constrained along with the expansion of the Asian lineage. Furthermore, between the African and Asian lineages, differentiated and specific over-represented codons were more prominent in the NS2B and NS4A. Together, our study implies that ZIKVs are in the process of evolutionary fine tuning their codon as seen in the recent pandemics, and NS2B and NS4A could have played a potential role in the molecular evolution of the Asian lineage and their establishment. [ABSTRACT FROM AUTHOR]

Manganese transport protein C (MntC) of Staphylococcus aureus represents an excellent vaccine-candidate antigen. The important role of CD4 + T cells in effective immunity against S. aureus infection was shown; however, CD4 + T cell-specific epitopes on S. aureus MntC have not been well identified. Here, we used bioinformatics prediction algorithms to evaluate and identify nine candidate epitopes within MntC. Our results showed that peptide M8 emulsified in Freund's adjuvant induced a much higher cell-proliferation rate as compared with controls. Additionally, CD4 + T cells stimulated with peptide M8 secreted significantly higher levels of interferon-γ and interleukin-17A. These results suggested that peptide M8 represented an H-2d (I-E)-restricted Th17-specific epitope. [ABSTRACT FROM AUTHOR]

Objective: To compare the prevalence of polycystic ovary syndrome (PCOS) and related clinical characteristics between metabolically unhealthy obese (MUO) and metabolically healthy obese (MHO) women of reproductive age.Design: Cross-sectional clinical study.Setting: Tertiary hospital.Patient(s): We studied 299 MUO and 122 MHO Chinese women matched on body mass index. Metabolically healthy obese was defined as obesity with no more than one metabolic abnormality. Diagnosis of PCOS was based on the revised Rotterdam criteria.Intervention(s): Each subject underwent physical examination, laboratory evaluation, and gynecologic ultrasound for a diagnosis of PCOS or metabolic syndrome (MetS).Main Outcome Measure(s): Prevalence of PCOS was calculated in both groups. Insulin resistance was determined by homeostasis model assessment of insulin resistance or by the insulin sensitivity index derived from Bergman's minimal model. Fat distribution was measured with computerized tomography scan.Result(s): Prevalence of PCOS and its components did not differ between MUO and BMI-matched MHO groups (67.89% and 66.96%, respectively). In logistic regression analysis, MetS did not predict the presence of PCOS after adjusting for confounding factors. The MHO group had lower visceral adipose tissue, relatively higher insulin sensitivity, and better β-cell function, compared with those in the MUO group; but there were no significant differences in sex hormones (except for free T and sex hormone-binding globulin) and ultrasound manifestations between MHO and MUO women.Conclusion(s): For the first time, our findings suggest that MetS does not add additional risk for PCOS. In addition, we found that both MUO and MHO are associated with insulin resistance to some extent. [ABSTRACT FROM AUTHOR]

The removal effect of nitrogen, sulfur and chlorine from the excavated waste utilizing the microwave-assisted hydrothermal method was investigated in this work. Speciation changes of nitrogen, sulfur and chlorine in hydrochars with different treatment times were determined by X-ray photoelectron spectroscopy analysis. Results showed that 90% chlorine, 65% nitrogen and 63% sulfur were removed from the excavated waste after treatment at 230 °C for 100 min, while 82% energy and 85% carbon element were recovered at the same time. The conversion of organic nitrogen, sulfur and chlorine into the corresponding water-soluble inorganic forms was enhanced by the combined microwave irradiation and hydrothermal reaction, which promoted the migration of Cl, S, N into the liquid phase. However, some original and generated species of nitrogen, sulfur and chlorine remained in the hydrochars due to the polymerization of organic forms and the adsorption of inorganic forms. These findings suggested that the efficient purification of nitrogen, sulfur and chlorine in excavated waste could be obtained using the microwave-assisted hydrothermal treatment without additives, which provided a reference for the recycling and cleaning utilization of excavated waste. [Display omitted] [ABSTRACT FROM AUTHOR]

The dissimilatory Fe(III)-reducing bacteria play a significant role in the mobility of antimony (Sb) under reducing environment. Sb-rich smelting slag is iron (Fe)-containing antimonic mine waste, which is one of the main sources of antimony pollution. In this study, the soluble antimony reacted with Fe(III) by S. oneidensis (Shewanella oneidensis strain MR-1) was performed in reduction condition, then the dissolution behavior of the Sb-rich smelting slag with S. oneidensis was investigated. The results showed that the released Sb was immobilized by S. oneidensis and the strain adsorbed Sb(III) preferentially. Sb(V) can be reduced by S. oneidensis without aqueous Fe. In the presence of Fe(III), S. oneidensis mediated Sb bio-adsorption and the chemical redox of Sb-Fe occurred simultaneously. Sb was co-precipitated with Fe to form the Sb(V)-O-Fe(III) secondary mineral, which was identified as the bidentate mononuclear edge-sharing structure by extended X-ray absorption fine structure (EXAFS) analysis. These results suggest that S. oneidensis has a positive effect on the immobilization and minimizing toxicity of antimony in anoxic soil and groundwater, which provides a theoretical basis for the treatment of antimony contamination. [Display omitted] • Sb was immobilized by S. oneidensis and Sb(III) was bio-adsorbed preferentially. • Sb(III) was indirectly oxidized to Sb(V) by S. oneidensis with aqueous Fe(III). • Sb was co-precipitated with the released Fe from the Sb-rich smelting slag. • Secondary mineral was bidentate mononuclear structure with Sb(V)-O-Fe(III). [ABSTRACT FROM AUTHOR]

Sufficient interfacial contact and adhesion are important factors for ensuring effective load transfer in carbon nanotubes (CNTs)-reinforced copper matrix composites (CMCs). In this study, a chemical unzipping method and matrix-alloying (addition of Cr) were integrated to solve these two challenges in CMCs. The results showed that partially-unzipped CNTs (PUCNTs) improved the restricted interfacial contact areas of CNTs. Furthermore, the graphene layers of PUCNTs might improve the interfacial shear strength, and fully utilize the load transfer capacity of the inner walls. Trace amounts of interfacial carbides (Cr 7 C 3 or Cr 23 C 6) were formed in situ at the PUCNTs/CuCr interface, which further improved the interfacial bonding between PUCNTs and the CuCr matrix. The ultimate tensile strength (382.9 MPa) and elongation (37.02%) of the 2 vol% PUCNTs/CuCr composite were higher than similar reported materials. A balance between the strength and ductility of the PUCNTs/CuCr composites was obtained. This was ascribed to the combined effects of the interfacial carbides and interfacial contact area, which improved the load transfer ability and interfacial adhesion, and also promoted dislocation accumulation ability of the PUCNTs. The strengthening effect of PUCNTs was also investigated using the correctional shear-lag strengthening and dislocation strengthening models. This work shows that PUCNTs are better reinforcement fillers than CNTs for enhancing the mechanical properties of CMCs. [ABSTRACT FROM AUTHOR]

Salicylate preservatives are widely used in consumer products and pharmaceuticals. This study investigates their potential endocrine-disrupting effects on neurosteroidogenesis, focusing on 5α-reductase type 1 (SRD5A1). We evaluated the effects of 13 salicylates on human SRD5A1 using SF126 glioblastoma cell microsomes and rat brain microsomes, examining dihydrotestosterone production in SF126 cells. Results revealed a hierarchy of inhibitory potency against human SRD5A1, with methyl salicylate (IC 50 , 71.93 μM) to menthyl salicylate (2.41 μM), indicating increasing potency. Kinetic analysis indicates their mixed/noncompetitive inhibitions. In SF126 cells, all salicylates at 100 μM significantly reduced dihydrotestosterone production. Rat SRD5A1 showed reduced sensitivity, with menthyl salicylate as the most potent inhibitor (IC 50 , 17.12 μM). Docking analysis suggests salicylates bind to the reduced nicotinamide adenine dinucleotide phosphate site of both human and rat SRD5A1. Bivariate correlation analysis highlights the influence of LogP, molecular weight, carbon number in the alcohol moiety, and p K a on inhibitory potency. 3D-QSAR revealed the importance of hydrophobic aromatic regions in SRD5A1 binding. This study delineates the inhibitory effects of salicylates and binding mechanisms on human and rat SRD5A1, providing insights into their impact on neurosteroid production.

Despite the monumental success of immunotherapy in treating melanoma clinically, it still confronts significant challenges, chiefly that singular immunomodulatory tactics are insufficient to suppress the recurrence and metastasis of melanoma. Herein, these challenges are addressed by a hydrogel based on M1 macrophage lysate and alginate (M1LMHA) loaded with oxaliplatin (OXA), named M1LMHA@OXA.The results obtained from scanning electron microscopy and confocal microscopy indicate that the structure and morphology of M1LMHA@OXA remain unchanged. Flow cytometry results reveal that M1LMHA@OXA significantly promotes the maturation of dendritic cells (DCs) and enhances the proliferation of T lymphocytes. In a subcutaneous melanoma transplant model, M1LMHA@OXA effectively suppressed tumor growth in comparison to OXA alone and M1LMHA alone. Flow cytometry demonstrated that M1LMHA@OXA markedly increased the number of mature DCs and CD8 + T cells at the tumor site, while significantly reducing the quantity of M2-like tumor-associated macrophages (TAM) and enhancing the presence of M1 macrophages. Enzyme-linked immunosorbent assay (ELISA) results indicated that following treatment with M1LMHA@OXA, the levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in the bloodstream of mice were significantly elevated, whereas interleukin-10 (IL-10) exhibited no significant difference. This outcome further corroborates the ability of M1LMHA@OXA to substantially bolster the immune capability of mice. Similar results have also been observed in a melanoma subcutaneous transplantation recurrence model, and optical imaging of the lungs of mice revealed that M1LMHA@OXA inhibited tumor metastasis to the lungs. Notably, M1LMHA@OXA exhibits an exceptional therapeutic effect on the growth, post-surgical recurrence, and metastasis of the B16F10 melanoma. Therefore, this study provides a straightforward strategy that leverages the cooperative regulation of multiple immune cells to thwart the proliferation, recurrence, and spread of melanoma., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Objectives: Magnetic resonance imaging (MRI) is increasingly used to detect knee osteoarthritis (KOA). In this study, we aimed to systematically examine the global research status on the application of medical knee MRI in the treatment of KOA, analyze research hotspots, explore future trends, and present results in the form of a knowledge graph., Methods: The Web of Science core database was searched for studies on medical knee MRI scans in patients with KOA between 2004 and 2023. CiteSpace, SCImago Graphica, and VOSviewer were used for the country, institution, journal, author, reference, and keyword analyses., Results: A total of 2,904 articles were included. The United States and Europe are leading countries. Boston University is the main institution. Osteoarthritis and cartilage is the main magazine. The most frequently cocited article was "Radiological assessment of osteoarthrosis". Guermazi A was the author with the highest number of publications and total references. The keywords most closely linked to MRI and KOA were "cartilage", "pain", and "injury"., Conclusions: The application of medical knee MRI in KOA can be divided into the following parts: (1). MRI was used to assess the relationship between the characteristics of local tissue damage and pathological changes and clinical symptoms. (2).The risk factors of KOA were analyzed by MRI to determine the early diagnosis of KOA. (3). MRI was used to evaluate the efficacy of multiple interventions for KOA tissue damage (e.g., cartilage defects, bone marrow edema, bone marrow microfracture, and subchondral bone remodeling). Artificial intelligence, particularly deep learning, has become the focus of research on MRI applications for KOA., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2024 Chen, Xu, Zhou, Wang, Li, Guo and Zhou.)