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765 results on '"Middeldorp, Jaap"'

202. Morphological Evidence of an Activated Cytotoxic T-Cell Infiltrate in EBV-Positive Gastric Carcinoma Preventing Lymph Node Metastases

Author

van Beek, Josine, primary, Hausen, Axel zur, additional, Snel, Sander N, additional, Berkhof, Johannes, additional, Kranenbarg, Elma Klein, additional, van de Velde, Cornelis J. H, additional, van den Brule, Adriaan J. C, additional, Middeldorp, Jaap M, additional, Meijer, Chris J. L. M, additional, and Bloemena, Elisabeth, additional

Published
2006
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217. Human cytomegalovirus immediate-early mRNAemia versus pp65 antigenemia for guiding pre-emptive therapy in children and young adults undergoing hematopoietic stem cell transplantation: a prospective, randomized, open-label trial

Author

Gerna, Giuseppe, primary, Lilleri, Daniele, additional, Baldanti, Fausto, additional, Torsellini, Maria, additional, Giorgiani, Giovanna, additional, Zecca, Marco, additional, De Stefano, Piero, additional, Middeldorp, Jaap, additional, Locatelli, Franco, additional, and Revello, M. Grazia, additional

Published
2003
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225. High numbers of granzyme B/CD8‐positive tumour‐infiltrating lymphocytes in nasopharyngeal carcinoma biopsies predict rapid fatal outcome in patients treated with curative intent

Author

Oudejans, Joost J, primary, Harijadi, Hari, additional, Kummer, J Alain, additional, Tan, I Bing, additional, Bloemena, Elizabeth, additional, Middeldorp, Jaap M, additional, Bladergroen, Belinda, additional, Dukers, Danny F, additional, Vos, Wim, additional, and Meijer, Chris JLM, additional

Published
2002
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227. A Rapid and Reliable Enzyme Immunoassay PCR-Based Screening Method to Identify EBV-Carrying Gastric Carcinomas

Author

van Beek, Josine, primary, zur Hausen, Axel, additional, Kranenbarg, Elma Klein, additional, Warring, Ralph J., additional, Bloemena, Elisabeth, additional, Craanen, Mikael E., additional, van de Velde, Cornelis J.H., additional, Middeldorp, Jaap M., additional, Meijer, Chris J.L.M., additional, and van den Brule, Adriaan J.C., additional

Published
2002
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233. Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR.

Author

Walker, John M., Lieberman, Paul M., Stevens, Servi J. C., Brink, Antoinette A. T. P., and Middeldorp, Jaap M.

Abstract

We describe a two-step RT-PCR method for simultaneous detection of EBNA-1 (QK and Y3K splice variants), EBNA-2, LMP-1, LMP-2a and -2b, ZEBRA, and BARTs RNA encoded by Epstein-Barr virus. As a control for RNA integrity, the low-copy-number transcript derived from U1 A snRNP, a cellular housekeeping gene, is coamplified. Copy DNA (cDNA) for these nine targets is simultaneously synthesized in a gene-specific, multiprimed cDNA reaction, which strongly reduces the amount of required clinical specimen and allows more sensitive detection than random hexamer or oligo-dT priming. For amplification, cDNA synthesis is followed by nine separate PCRs for the mentioned targets. Primers were designed either as intron-flanking, to avoid background DNA amplification, or in different exons, allowing identification of differentiallly spliced RNA molecules. To increase specificity, PCR products are detected by autoradiography after hybridization with radiolabeled internal oligonucleotide probes. The method described is highly suitable for profiling EBV latent RNA expression in tissue biopsies, cultured or isolated cells, and unfractionated whole blood and for definition of EBV latency type I, II, or III gene expression in these samples. [ABSTRACT FROM AUTHOR]

Published
2005
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234. Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1.

Author

Walker, John M., Lieberman, Paul M., Stevens, Servi J. C., Verkuijlen, Sandra A. W. M., and Middeldorp, Jaap M.

Abstract

Here we describe a LightCycler-based real-time PCR for quantitative detection of EBV DNA in clinical samples such as unfractionated whole blood, serum, or plasma. This assay is based on amplification of a highly conserved 213-bp region of the EBNA-1 gene, a single-copy gene of EBV required for maintenance of the EBV genome within the infected host cell. For real-time detection of amplicons, two internal hybridization probes are added, labeled with the fluoregenic dyes fluorescein and LCRed640, respectively. Simultaneous hybridization of these probes to the amplification products brings them in close proximity. Subsequent excitation of the fluorescein label by filtered excitation light from a light source in the LightCycler device will lead to fluorescence energy transfer (FRET) from the fluorescein label to the LCRed640 label. The light emitted from the LCRed640 label is then measured and correlates to the amount of product generated. The cycle at which the fluorescence exceeds the background is designated the threshold cycle. By comparing the threshold cycle of a clinical specimen with those of standard curve samples, the amount of EBV DNA in clinical samples can be determined. This real-time PCR approach is extremely rapid owing to efficient heat conduction by using glass capillaries, small reaction volumes, and air as heating medium. The "closed-tube" system eliminates the risk of PCR contamination by product carryover and also the need for post-PCR detection. [ABSTRACT FROM AUTHOR]

Published
2005
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240. EVALUATION OF HUMAN CYTOMEGALOVIRUS GENE EXPRESSION IN THORACIC ORGAN TRANSPLANT RECIPIENTS USING NUCLEIC ACID SEQUENCE-BASED AMPLIFICATION

Author

Oldenburg, Nienke, primary, Lam, Kitty M. C., additional, Khan, Mahboob A., additional, Top, Bert, additional, Tacken, Nicole M. M., additional, McKie, Anne, additional, Mikhail, Ghada W., additional, Middeldorp, Jaap M., additional, Wright, Andrew, additional, Banner, Nicholas R., additional, and Yacoub, Magdi, additional

Published
2000
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243. Human Cytomegalovirus Immediate-Early mRNA Detection by Nucleic Acid Sequence-Based Amplification as a New Parameter for Preemptive Therapy in Bone Marrow Transplant Recipients

Author

Gerna, Giuseppe, primary, Baldanti, Fausto, additional, Lilleri, Daniele, additional, Parea, Maurizio, additional, Alessandrino, Emilio, additional, Pagani, Ambrogio, additional, Locatelli, Franco, additional, Middeldorp, Jaap, additional, and Revello, M. Grazia, additional

Published
2000
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246. Combination of SAHA and bortezomib up-regulates CDKN2A and CDKN1A and induces apoptosis of Epstein-Barr virus-positive Wp-restricted Burkitt lymphoma and lymphoblastoid cell lines.

Author

Hui, Kwai Fung, Leung, Yvonne Y., Yeung, Po L., Middeldorp, Jaap M., and Chiang, Alan K.S.

Subjects
BURKITT'S lymphoma, LYMPHOBLASTOID cell lines, EPSTEIN-Barr virus, BORTEZOMIB, HYDROXAMIC acids, APOPTOSIS, HISTONE deacetylase inhibitors, CYCLIN-dependent kinases
Abstract

Epstein-Barr virus (EBV) latent proteins exert anti-apoptotic effects on EBV-transformed lymphoid cells by down-regulating BCL2L11 (BIM), CDKN2A (p16INK4A) and CDKN1A (p21WAF1). However, the potential therapeutic effects of targeting these anti-apoptotic mechanisms remain unexplored. Here, we tested both in vitro and in vivo effects of the combination of histone deacetylase (HDAC) and proteasome inhibitors on the apoptosis of six endemic Burkitt lymphoma (BL) lines of different latency patterns (types I and III and Wp-restricted) and three lymphoblastoid cell lines (LCLs). We found that the combination of HDAC and proteasome inhibitors (e.g. SAHA/bortezomib) synergistically induced the killing of Wp-restricted and latency III BL and LCLs but not latency I BL cells. The synergistic killing was due to apoptosis, as evidenced by the high percentage of annexin V positivity and strong cleavage of PARP1 (PARP) and CASP3 (caspase-3). Concomitantly, SAHA/bortezomib up-regulated the expression of CDKN2A and CDKN1A but did not affect the level of BCL2L11 or BHRF1 (viral homologue of BCL2). The apoptotic effects were dependent on reactive oxygen species generation. Furthermore, SAHA/bortezomib suppressed the growth of Wp-restricted BL xenografts in nude mice. This study provides the rationale to test the novel application of SAHA/bortezomib on the treatment of EBV-associated Wp-restricted BL and post-transplant lymphoproliferative disorder. [ABSTRACT FROM AUTHOR]

Published
2014
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247. Epstein-Barr virus viral load and serology in childhood non-Hodgkin's lymphoma and chronic inflammatory conditions in Uganda: Implications for disease risk and characteristics.

Author

Orem, Jackson, Sandin, Sven, Mbidde, Edward, Mangen, Fred Wabwire, Middeldorp, Jaap, and Weiderpass, Elisabete

Abstract

Epstein-Barr virus (EBV) has been linked to malignancies and chronic inflammatory conditions. In this study, EBV detection was compared in children with non-Hodgkin's lymphoma and children with chronic inflammatory conditions, using samples and data from a case-control study carried out at the Mulago National Referral Hospital between 2004 and 2008. EBV viral load was measured in saliva, whole blood and white blood cells by real-time PCR. Serological values for IgG-VCA, EBNA1, and EAd-IgG were compared in non-Hodgkin's lymphoma and chronic inflammatory conditions; and in Burkitt's lymphoma and other subtypes of non-Hodgkin's lymphoma. Odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were calculated. Of the 127 children included (87 males and 40 females; median age 7 years, range 2-17), 96 had non-Hodgkin's lymphoma (46 Burkitt's lymphoma and 50 other non-Hodgkin's lymphoma), 31 had chronic inflammatory conditions, and only 10% were HIV-positive. The most common clinical presentations for all disease categories considered were fever, night sweats, and weight loss. EBV viral load in whole blood was elevated in Burkitt's lymphoma compared to other non-Hodgkin's lymphoma (OR 6.67, 95% CI 1.32, 33.69; P-value = 0.04), but EBV viral loads in saliva and white blood cells were not different in any of the disease categories considered. A significant difference in EAd-IgG was observed when non-Hodgkin's lymphoma was compared with chronic inflammatory conditions (OR 0.19, 95% CI 0.07, 0.51; P-value = 0.001). When compared to chronic inflammatory conditions, EBV viral load was elevated in Burkitt's lymphoma, and EA IgG was higher in non-Hodgkin's lymphoma. This study supports an association between virological and serological markers of EBV and childhood non-Hodgkin's lymphoma, irrespective of subtype, in Uganda. J. Med. Virol. 86: 1796-1803, 2014. © 2014 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Published
2014
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