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We investigated whether the alteration of the motor unit recruitment threshold (MURT) caused by quercetin ingestion intervention for 7 days modifies motor unit activation patterns before and after a single session of resistance exercise. Twenty young male and female adults were divided into two groups: ingestion of placebo (PLA) or quercetin glycosides at 200 mg/day (QUE). High-density surface electromyography during submaximal contractions was measured to assess the motor unit firing rate (MUFR) and MURT of the vastus lateralis muscle before (PRE) and after (POST) resistance exercise (DAY1). The same measurements were repeated after 7 days of placebo or quercetin glycoside ingestion (DAY8). In QUE, MURT decreased more from DAY1-PRE to DAY8-PRE (29.1 ± 9.1 to 27.1 ± 9.5% MVC, p  < 0.001) but not in PLA (29.8 ± 10.4 to 28.9 ± 9.7% MVC, p  < 0.167). For percentage change in MUFR following resistance exercise, there was a significant interaction (day × group, p  < 0.001). The degree of changes in MURT from DAY1-PRE to DAY8-PRE was significantly correlated with the percentage change of MUFR from DAY8-PRE to DAY8-POST in QUE ( p  = 0.014, r = -0.363) but not in PLA ( p  = 0.518). The study suggests that 7-day quercetin ingestion alters the motor unit recruitment pattern, and this may induce changes in motor unit firing patterns during a single session of resistance training (Trial registration: UMIN000052255, R000059650)., Competing Interests: No conflicts of interest, financial or otherwise, are declared by the authors

Several methods have been put forward to quantify cumulative loads; however, limited evidence exists as to the subsequent damages and the role of muscular fatigue. The present study assessed whether muscular fatigue could affect cumulative damage imposed on the L5-S1 joint. Trunk muscle electromyographic (EMG) activities and kinematics/kinetics of 18 healthy male individuals were evaluated during a simulated repetitive lifting task. A traditional EMG-assisted model of the lumbar spine was modified to account for the effect of erector spinae fatigue. L5-S1 compressive loads for each lifting cycle were estimated based on varying (i.e. actual), fatigue-modified, and constant Gain factors. The corresponding damages were integrated to calculate the cumulative damage. Moreover, the damage calculated for one lifting cycle was multiplied by the lifting frequency, as the traditional approach. Compressive loads and the damages obtained through the fatigue-modified model were predicted in close agreement with the actual values. Similarly, the difference between actual damages and those driven by the traditional approach was not statistically significant ( p  = 0.219). However, damages based on a constant Gain factor were significantly greater than those based on the actual ( p  = 0.012), fatigue-modified ( p  = 0.017), and traditional ( p  = 0.007) approaches. Practitioner summary: In this study, we managed to include the effect of muscular fatigue on cumulative lumbar damage calculations. Including the effect of muscular fatigue leads to an accurate estimation of cumulative damages while eliminating computational complexity. However, using the traditional approach also appears to provide acceptable estimates for ergonomic assessments.

Purpose: To determined sex differences in absolute- and %-reductions in blood flow during intermittent muscular contractions as well as relationships between blood flow reductions and time to task failure (TTF)., Methods: Thirteen males (25 ± 4 years) and 13 females (22 ± 5 years) completed intermittent isometric trapezoidal forearm flexion at 50% maximal voluntary contraction until task failure. Doppler ultrasound was used to measure brachial artery blood flow (BABF) during the 12-s plateau phase and 12-s relaxation phase., Results: Target torque was less in females than males (24 ± 5 vs. 42 ± 7 Nm; p < 0.001); however, TTF was not different between sexes (F: 425 ± 187 vs. M: 401 ± 158 s; p = 0.72). Relaxation-phase BABF at end-exercise was less in females than males (435 ± 161 vs. 937 ± 281 mL/min; p < 0.001) but contraction-phase BABF was not different (127 ± 46 vs. 190 ± 99 mL/min; p = 0.42). Absolute- and %-reductions in BABF by contraction were less in females than males (309 ± 146 vs. 747 ± 210 mL/min and 69 ± 10 vs. 80% ± 6%, respectively; both p < 0.01) and were associated with target torque independent of sex (r = 0.78 and 0.56, respectively; both p < 0.01). Absolute BABF reduction per target torque (mL/min/Nm) and TTF were positively associated in males (r = 0.60; p = 0.031) but negatively associated in females (r = - 0.61; p = 0.029)., Conclusions: This study provides evidence that females incur less proportional reduction in limb blood flow from muscular contraction than males at a matched relative intensity suggesting females may maintain higher levels of muscle oxygen delivery and metabolite removal than males across the contraction-relaxation cycle of intermittent exercise., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Background: Kinesiology Taping(KT) is commonly used as a physical therapy to prevent exercise-induced fatigue. This study aims to evaluate the immediate effects of KT on muscle strength, static balance, and proprioception after eccentric muscle fatigue on ankle., Methods: Twenty healthy male university students were recruited. The experimental protocol was structured into four sessions, each separated by a one-week washout period to prevent carryover effects. Participants were randomly allocated to one of four intervention conditions in each session, ensuring no participant received the same intervention twice. These conditions were: no taping(NT),sham taping(ST),athletic taping(AT),and kinesiology taping(KT).Taping was applied immediately following an eccentric muscle fatigue protocol targeting the ankle, and assessments were conducted in the order of proprioception, muscle strength and static balance. Isometric muscle strength and proprioception were evaluated using the Biodex isokinetic system. Static balance was measured using the TecnoBody balance platform., Results: KT had a significantly higher plantarflexion/dorsiflexion peak torque, dorsiflexion average peak torque, and plantarflexion/dorsiflexion average power at 60°/s compared with NT and ST in terms of isometric muscle strength (p < 0.05).Furthermore, the plantarflexion peak torque of KT was significantly greater than AT at 60°/s[p = 0.005,95% confidence interval(CI) = 3.39 to 18.20] and 180°/s[p = 0.006,95%CI(2.62,21.98)]. In terms of proprioception, KT showed a lower absolute error in 25° plantarflexion and 10° dorsiflexion compared to NT, ST and AT. For static balance with eyes-open and eyes-closed conditions, AT and KT had a lower total sway area than NT and ST (p < 0.05). Additionally, a significant difference in total sway length with eyes-open condition was observed between AT and KT[p < 0.001,95%CI(-431.81,-168.25)];total sway area and the center of pressure(COP) velocity in the mediolateral(ML) and anteroposterior(AP) directions with eyes-closed condition were significantly lower in AT compared to KT., Conclusion: This study suggests that KT is more effective than other taping conditions in improving muscle strength and proprioception after eccentric muscle fatigue on ankle. However, AT is more helpful in increasing static postural control ability after ankle muscle fatigue than KT., Trial Registration: This study was registered with www.chictr.org.cn (registration number: ChiCTR2300068278) on 13/2/2023., (© 2024. The Author(s).)

Background and Objectives : Lean body mass loss after bariatric surgery (BS) is remarkable, despite an effective long-term mass reduction and significant declines in comorbidities. A person's functional capacity is adversely affected when their skeletal muscle strength declines by up to 30%. This study aimed to assess the isokinetic trunk muscle strength and fatigue rate in individuals after BS. Materials and Methods : This study included fifty-eight patients, both male and female, ranging in age from 19 to 45. Twenty-seven individuals had BS and twenty-seven healthy people served as the control group. The primary outcomes were the measurement of the concentric and eccentric isokinetic muscle strength of the trunk flexor and extensor muscles. An isokinetic dynamometer (Biodex Rehabilitation and Testing System 3) was used for the assessment of the isokinetic muscle strength. Noraxon EMG was used to determine a secondary outcome, which was the median frequency slop (MF/time) and root mean square slop (RMS/time) of the lumbar erector spinea muscle at 50% of the Maximum Voluntary Isometric Contraction (MVIC). Outcome measures were assessed for both groups. Results : Compared to the control group, the bariatric group showed a lower mean value of both concentric and eccentric isokinetic muscle strength for the flexor and extensor trunk muscles ( p < 0.05). In terms of the EMG fatigue rate, the RMS slope increased significantly more than that of the control group, while the MF slope decreased ( p > 0.05). Conclusions : The current study found that, in comparison to the healthy subjects, the BS group showed reduced levels of fatigue and isokinetic strength in the trunk muscles. Based on these results, it is recommended that individuals who underwent BS take part in tailored rehabilitation programs to avoid potential musculoskeletal issues in the future.

This study aimed to test the hypotheses that fatiguing unilateral handgrip contraction exhibits different changes in corticomotor excitability, which is evaluated by motor-evoked potentials (MEPs), in the lower limbs ipsilateral and contralateral to the fatigued hand, and that the changes in corticomotor excitability estimated by MEPs in the non-fatigued lower limbs affect the force exerted based on the sense of effort. Ten healthy males completed fatiguing unilateral handgrip contraction and force-matching tasks by static dorsiflexion before, immediately after, and 10 min after handgrip contraction. MEPs in the tibialis anterior (TA) were also measured before, immediately after, and 10 min after handgrip contraction. Fatiguing handgrip contractions resulted in asymmetrical MEPs in the TA muscles. Specifically, MEPs in the contralateral TA muscle were significantly increased (158 ± 60 %) and MEPs in the contralateral TA muscle were greater after the handgrip contraction than the ipsilateral MEPs (111 ± 30 %). Moreover, the torque exerted during the force-matching task significantly increased only in the contralateral ankle after the fatiguing handgrip contraction. Fatiguing unilateral handgrip contraction results in asymmetric changes in corticomotor excitability in the TA muscle, and the force exerted during the force-matching task based on the sense of effort becomes higher in the TA muscle with greater corticomotor excitability than that before fatiguing unilateral handgrip contraction., Competing Interests: Declaration of competing interest There are no conflicts of interest regarding this manuscript., (Copyright © 2023. Published by Elsevier Inc.)

Purpose: This study aimed to examine if peripheral fatigue is adjusted during knee extensor (KE) exercise in order not to surpass a critical threshold patient with type 1 diabetes (T1D) and the consequences of this mechanism on the force-duration relationship., Methods: Eleven T1D individuals randomly performed two different sessions in which they performed 60 maximum voluntary contractions (MVC; 3 s contraction, 2 s relaxation). One trial was performed in the non-fatigued state (CTRL) and another after fatiguing neuromuscular stimulation of the KE (FNMES). Peripheral and central fatigue were quantified by the difference between pre and post exercise in quadriceps voluntary activation (ΔVA) and potentiated twitch (ΔPtw). Critical torque (CT) was determined as the average force of the last 12 contractions, whereas W' was calculated as the area above the CT., Results: Although FNMES led to a significant decrease in potentiated twitch (Ptw) before performing the 60-MVCs protocol (p < 0.05), ΔVA (∼ -7.5%), ΔP tw (∼ -39%), and CT (∼816 N) post-MVCs were similar between the two conditions. The difference in W' between CTRL and FNMES was correlated with the level of pre-fatigue induced in FNMES (r 2  = 0.60). In addition, W' was correlated with ΔP tw (r 2  = 0.62) in the CTRL session., Conclusion: Correlative results in the present study indicate that regulating peripheral fatigue mechanisms at a critical threshold limit W'. Additionally, peripheral fatigue during KE exercise is limited to an individual threshold in T1D patients., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Measuring muscle fatigue involves assessing various components within the motor system. While subjective and sensor-based measures have been proposed, a comprehensive comparison of these assessment measures is currently lacking. This study aims to bridge this gap by utilizing three commonly used measures: participant self-reported perceived muscle fatigue scores, a sports physiotherapist's manual palpation-based muscle tightness scores, and surface electromyography sensors. Compensatory muscle fatigue occurs when one muscle group becomes fatigued, leading to the involvement and subsequent fatigue of other muscles as they compensate for the workload. The evaluation of compensatory muscle fatigue focuses on nine different upper body muscles selected by the sports physiotherapist. With a cohort of 30 male subjects, this study provides a valuable dataset for researchers and healthcare practitioners in sports science, rehabilitation, and human performance. It enables the exploration and comparison of diverse methods for evaluating different muscles in isometric contraction., (© 2024. The Author(s).)

After a unilateral muscle exercise, the performance of the non-exercised contralateral limb muscle can be also impaired. This crossover fatigue phenomenon is still debated in the literature and very few studies have investigated the influence of eccentric contractions. This study was designed to assess neuromuscular adaptations involved in the crossover fatigue of the non-exercised contralateral knee flexor muscles. Seventeen healthy young men performed a unilateral submaximal eccentric exercise of the right knee flexors until a 20% reduction in maximal voluntary isometric contraction torque was attained in the exercised limb. Before (PRE), immediately after exercise cessation (POST) and 24 hours later (POST24), neuromuscular function and perceived muscle soreness were measured in both the exercised limb and non-exercised limb. In addition, global perceived fatigue was assessed at each measurement time. At POST, significant reductions in maximal voluntary isometric contraction were observed in the exercised limb (-28.1%, p < 0.001) and in the non-exercised limb (-8.5%, p < 0.05), evidencing crossover fatigue. At POST, voluntary activation decreased in the exercised limb only (-6.0%, p < 0.001), while electrically evoked potentiated doublet torque was impaired in both the exercised limb and the non-exercised limb (-11.6%, p = 0.001). In addition, global perceived fatigue significantly increased at POST (p < 0.001). At POST24, all measured variables returned to PRE values, except for perceived muscle soreness scores exhibiting greater values than PRE (p < 0.05). A possible cumulative interaction between peripheral alterations and global perceived fatigue may account for the immediate crossover fatigue observed in the non-exercised limb., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Gioda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The Biering-Sørensen test is commonly used to assess paraspinal muscle endurance. Research using a single repetition of the test has provided conflicting evidence for the contribution of impaired paraspinal muscle endurance to low back pain (LBP). This study investigated how Sørensen test duration, muscle activation, and muscle fatigability are affected by multiple repetitions of the test and determined predictors of Sørensen test duration in young adults with and without a history of LBP. Sixty-four young individuals performed three repetitions of the Sørensen test. Amplitude of activation and median frequency slope (fatigability) were calculated for the lumbar and thoracic paraspinals and hamstrings. Duration of the test was significantly less for the 3rd repetition in individuals with LBP. In individuals without LBP, test duration was predicted by fatigability of the lumbar paraspinals. In individuals with LBP, Sørensen test duration was predicted by fatigability of the hamstrings and amplitude of activation of the thoracic and lumbar paraspinals. Our findings demonstrate that it is necessary to amplify the difficulty of the Sørensen test to reveal impairments in young, active adults with LBP. Training programs aiming to improve lumbar paraspinal performance should monitor performance of other synergist muscles during endurance exercise., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

We investigated whether a single heart rate clamped cycling session under systemic hypoxia affects the recovery of physical and psycho-physiological responses from residual fatigue compared to normoxia. On separate occasions, twelve trained males performed a 3-d acute training camp scenario. On days 1 and 3, participants cycled for 60 min at a constant heart rate (80% of ventilatory threshold). On day 2, fatigue was induced through a simulated team game circuit (STGC), followed by a 60-min intervention of either: (1) heart rate clamped cycling in normoxia; (2) heart rate clamped cycling in hypoxia (simulated altitude ~ 3500 m); or (3) no cycling. Countermovement jump height and leg stiffness were assessed before and after every session. Perceptual fatigue was evaluated daily. Compared to baseline, jump height decreased at all timepoints following the STGC (all p  < 0.05). Leg stiffness and cycling power output only decreased immediately following the STGC, with a 48% further decrease in cycling power output in hypoxia compared to normoxia ( p  < 0.05). Perceived fatigue, decreased sleep quality, and increased muscle soreness responses occurred on day 3 ( p  < 0.05). A single heart rate-clamped cycling session in hypoxia reduced mechanical output without affecting recovery of physical performance and perceptual measures from residual fatigue induced through team sport activity.

Rinsing the mouth with a carbohydrate (CHO) solution has been shown to enhance exercise performance while reducing neuromuscular fatigue. This effect is thought to be mediated through the stimulation of oral receptors, which activate brain areas associated with reward, motivation, and motor control. Consequently, corticomotor responsiveness is increased, leading to sustained levels of neuromuscular activity prior to fatigue. In the context of endurance performance, the evidence regarding the central involvement of mouth rinse (MR) in performance improvement is not conclusive. Peripheral mechanisms should not be disregarded, particularly considering factors such as low exercise volume, the participant's fasting state, and the frequency of rinsing. These factors may influence central activations. On the other hand, for strength-related activities, changes in motor evoked potential (MEP) and electromyography (EMG) have been observed, indicating increased corticospinal responsiveness and neuromuscular drive during isometric and isokinetic contractions in both fresh and fatigued muscles. However, it is important to note that in many studies, MEP data were not normalised, making it difficult to exclude peripheral contributions. Voluntary activation (VA), another central measure, often exhibits a lack of changes, mainly due to its high variability, particularly in fatigued muscles. Based on the evidence, MR can attenuate neuromuscular fatigue and improve endurance and strength performance via similar underlying mechanisms. However, the evidence supporting central contribution is weak due to the lack of neurophysiological measures, inaccurate data treatment (normalisation), limited generalisation between exercise modes, methodological biases (ignoring peripheral contribution), and high measurement variability.Trial registration: PROSPERO ID: CRD42021261714., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Females demonstrate greater fatigue resistance during a range of exercise modalities; however, this may be confounded by the lower mechanical work completed. Accordingly, this study examined the sex-specific peripheral and central fatigue mechanisms during repeated all-out cycling and whether they are affected by total mechanical work performed. A total of 26 healthy young adults (12 females) performed 10 × 10 s all-out cycling interspersed by 30 s passive recovery. Metabolic responses, peripheral and central fatigue, were quantified via changes in pre- to post-exercise blood lactate, potentiated quadriceps twitch force (and contractile properties) evoked via supramaximal electrical stimulation of the femoral nerve, and voluntary activation of the knee extensors, respectively. During exercise, mechanical work, vastus lateralis muscle activation (via surface electromyography), and deoxygenation (via near-infrared spectroscopy) were recorded. Sex comparison analyses were performed before and after statistically controlling for total mechanical work (via ANCOVA). Mechanical work and muscle activation plateaued at similar sprint repetition (sprint 5) and voluntary activation change (pre vs. post) was similar between the sexes. Females, however, showed lower %work decrement (i.e., fatigability; P  = 0.037) and peripheral responses as evident by lower reductions in quadriceps twitch force ( P  < 0.001) and muscle deoxygenation ( P  = 0.001). Adjusting for total mechanical work did not change these sex comparison results. We show that females' greater fatigue resistance during repeated all-out cycling may not be attributed to the greater total mechanical work performed but could be mediated by lower peripheral fatigue in the knee extensor muscles., Competing Interests: The authors declare there are no competing interests.

Muscle fatigue is prevalent across different aspects of daily life. Tracking muscle fatigue is useful to understand muscle overuse and possible risk of injury leading to musculoskeletal disorders. Current fatigue models are not suitable for real-world settings as they are either validated using simulations or non-functional tasks. Moreover, models that capture the changes to muscle activity due to fatigue either assume a linear relationship between muscle activity and muscle force or utilize a simple muscle model. Personalised electromygraphy (EMG)-driven musculoskeletal models (pEMS) offer person-specific approaches to model muscle and joint kinetics during a wide repertoire of daily life tasks. These models utilize EMG, thus capturing central fatigue-dependent changes in multi-muscle bio-electrical activity. However, the peripheral muscle force decay is missing in these models. Thus, we studied the influence of fatigue on a large scale pEMS of the trunk. Eleven healthy participants performed functional asymmetric lifting task. Average peak body-weight normalized lumbosacral moments (BW-LM) were estimated to be 2.55 ± 0.26 Nm/kg by reference inverse dynamics. After complete exhaustion of the lower back, the pEMS overestimated the peak BW-LM by 0.64 ± 0.37 Nm/kg. Then, we developed a time-varying muscle force decay model resulting in a time-varying pEMS (t-pEMS). This reduced the difference between BW-LM estimated by the t-pEMS and reference to 0.49 ± 0.14 Nm/kg. We also showed that five fatiguing contractions are sufficient to calibrate the t-pEMS. Thus, this study presents a person and muscle specific model to track fatigue during functional tasks., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Background: Reaction time is a reliable indicator of the velocity and efficiency of neuromuscular control and may be associated with fear-avoidance beliefs. However, the effect of exercise-induced muscle fatigue on reaction time in chronic low back pain (cLBP) and its relationship with fear-avoidance beliefs remains poorly understood., Objectives: This study aimed to reveal the relationship between fear-avoidance beliefs and reaction time changes before and after exercise-induced muscle fatigue in cLBP., Methods: Twenty-five patients with cLBP were tested by the Biering-Sorensen test (BST) to induce exhaustive muscle fatigue. Total reaction time (TRT), premotor time (PMT), and electromechanical delay (EMD) of dominated deltoid muscle were recorded by surface electromyography during the arm-raising task with visual cues before and after muscle fatigue. The mean difference (MD) of TRT (MD TRT ), PMT (MD PMT ), and EMD (MD EMD ) was calculated from the changes before and after muscle fatigue. Fear-avoidance beliefs questionnaire (FABQ) was applied to evaluate fear-avoidance beliefs before muscle fatigue. In addition, the duration time of BST was recorded for each subject., Results: TRT and PMT of dominated deltoid muscle were prolonged after exercise-induced muscle fatigue ( Z  = 3.511, p < 0.001; t  = 3.431, p = 0.001), while there was no statistical difference in EMD ( Z  = 1.029, p = 0.304). Correlation analysis showed that both the MD TRT and MD PMT were positively correlated with FABQ ( r  = 0.418, p = 0.042; r  = 0.422, p = 0.040)., Conclusions: These findings suggested that we should pay attention to both muscle fatigue-induced reaction time delay in cLBP management and the possible psychological mechanism involved in it. Furthermore, this study implied that FABQ-based psychotherapy might serve as a potential approach for cLBP treatment by improving reaction time delay. This trial is registered with ChiCTR2300074348., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2024 Wenwu Xiao et al.)

Purpose: Fran is one of the most popular CrossFit benchmark workouts used to control CrossFitters' improvements. Detailed physiological characterization of Fran is needed for a more specific evaluation of CrossFitters' training performance improvements. The aim of the study was to analyze the oxygen uptake (V˙O2) kinetics and characterize the energy system contributions and the degree of postexercise fatigue of the unbroken Fran., Methods: Twenty trained CrossFitters performed Fran at maximal exertion. V˙O2 and heart-rate kinetics were assessed at baseline and during and post-Fran. Blood lactate and glucose concentrations and muscular fatigue were measured at baseline and in the recovery period., Results: A marked increase in V˙O2 kinetics was observed at the beginning of Fran, remaining elevated until the end (V˙O2peak: 49.2 [3.7] mL·kg-1·min-1, V˙O2 amplitude: 35.8 [5.2] mL·kg-1·min-1, time delay: 4.7 [2.5] s and time constant: 23.7 [11.1] s; mean [SD]). Aerobic, anaerobic lactic, and alactic pathways accounted for 62% (4%), 26% (4%), and 12% (2%) of energy contribution. Reduction in muscle function in jumping ability (jump height: 8% [6%], peak force: 6% [4%], and maximum velocity: 4% [2%]) and plank prone test (46% [20%]) was observed in the recovery period., Conclusions: The Fran unbroken workout is a high-intensity effort associated with an elevated metabolic response. This pattern of energy response highlights the primary contribution of aerobic energy metabolism, even during short and very intense CrossFit workouts, and that recovery can take >24 hours due to cumulative fatigue.

Gum chewing is used in orofacial therapy to improve oral functions, such as a patient's chewing ability, bite force, tongue pressure, and lip closure strength. However, its effects on masseter muscle oxygen dynamics and muscle activity may vary with the hardness and features of the gum base. Therefore, when considering gum chewing for therapeutic purposes, it is essential to select gum of an appropriate hardness. This study aimed to elucidate the impact of gum hardness differences on masseter muscle tissue oxygen dynamics and muscle activity. We conducted a 120-s gum chewing study using three types of gum with different hardness levels on 11 healthy adult males. Each patient's masseter muscle tissue oxygen dynamics, muscle activity, and heart rate were measured, and the extent of masseter fatigue was assessed using a visual analogue scale (VAS). Per our findings, as gum hardness increased, significant reductions in oxygen saturation (StO 2 ) and significant increases in deoxyhaemoglobin (Deoxy-Hb) concentrations were observed in masseter muscle tissue oxygen dynamics. Likewise, muscle activity, heart rate, and muscle fatigue (according to VAS) also increased significantly as gum hardness increased. The findings of this study reveal that increasing gum base hardness not only affects masseter muscle tissue oxygen dynamics but also increases muscle activity, masseter fatigue, and heart rate. When selecting gum for orofacial therapy involving gum chewing exercises, it is crucial to choose the appropriate gum., (© 2024. Oxygen Transport to Tissue International.)

This study aimed to clarify the effect of 1 month of gum chewing (GCh) training on masseter muscle oxygen dynamics and determine its effectiveness in improving muscle endurance and fatigue. A total of 16 healthy adult subjects were included in this study. The subjects were randomly assigned to two groups: control and training groups (eight subjects each). GCh training using prototype gum of moderate hardness was performed for 10 min (5 min for each side) three times a day before each meal for 30 days. Clenching effects were evaluated before and after interventions. Masseter muscle oxygen dynamics were measured using spatially resolved near-infrared spectroscopy. Furthermore, masseter muscle fatigue was assessed using the Visual Analog Scale (VAS). After training, a significant increase was observed in total haemoglobin and oxygenated haemoglobin during clenching, and the 1/2 recovery time was significantly shortened. The changes in the VAS score showed a decreasing trend after training. In conclusion, 1 month of GCh training changed masseter oxygen dynamics during clenching and recovery and improved muscle aerobic capacity., (© 2024. Oxygen Transport to Tissue International.)

The purpose of this study was to clarify the characteristics of muscle fatigue by measuring the oxygenation of muscles during wheelchair sprinting in wheelchair basketball players, using near-infrared spectroscopy (NIRS). We believe that this information will be helpful in planning rehabilitation and training programmes.Five male wheelchair basketball players (mean age 30.0 ± 12.0 years, mean height 166.0 ± 12.1 cm, and mean weight 61.0 ± 15.8 kg) participated in this study.These participants performed six 20 m sprint tests after warming up. NIRS measurements were obtained at rest before the start of exercise, during sprinting movements, and during the recovery time after sprinting. Measurements were taken using a wireless NIRS device (PortaMon, Artinis, The Netherlands; sampling rate, 10 Hz) with a single-channel system for tissue oxygenation measurements. The NIRS sensor was applied to the triceps muscle on the participant's dominant arm and fixed with an upper arm supporter. The measurement data were imported into a dedicated computer and the tissue saturation index (TSI) was calculated. Statistical analysis was performed using the Friedman test to compare the TSI before sprinting and at each sprint, and a post hoc Bonferroni test was conducted.Compared to the pre-sprint measurements, TSI significantly decreased during sprints second, fourth, and fifth.In the present study, changes in the oxygen status of the triceps muscle during wheelchair driving varied greatly among individuals, and a tendency was observed towards the oxygen saturation being lowest by the fourth sprint, with the decrease being suppressed from this point on., (© 2024. Oxygen Transport to Tissue International.)

Vibration exercise (VE) has shown promising results for improving muscle strength and power performance when superimposed on high-level muscle contraction. However, low-level contraction may be more preferable in many rehabilitation programs due to the weakness of the patients. Unfortunately, the effects and underlying physiological mechanisms of VE superimposed on low-level contraction are unclear. This study aims to investigate the fatiguing effects and motor unit (MU) firing patterns during VE with low-level muscle contraction. Twenty-one healthy participants performed 60-s isometric contraction of the upper limb under a baseline force at 30% maximum voluntary contraction and superimposed vibration with an amplitude of 50% baseline and different frequencies at 0 Hz (control), 15, 25, 35, and 45 Hz. High-density surface electromyography (EMG) was recorded on the biceps brachii. The decay in muscle fiber conduction velocity, calculated in 3-s sliding windows, was employed as an indicator of myoelectric fatigue. MU firing patterns were obtained by decomposing the high-density EMG into MU spike trains. VE, particularly at 25 Hz, produces increased myoelectric fatigue as compared to the control condition. Besides, synchronized MU discharges are observed at the vibration frequency for 15- and 25-Hz VE and the sub-harmonics for 35- and 45-Hz VE. Furthermore, VE-induced increase in MU synchronization (as compared to control) seems to decrease with myoelectric fatigue. Significance: Our findings suggest that VE may be a suitable modality for rehabilitation programs, providing useful insights for subscribing appropriate VE training protocols.

Muscle fatigue impacts upper extremity function but is often overlooked in biomechanical models. The present work leveraged a transfer learning approach to improve torque predictions during fatiguing upper extremity movements. We developed two artificial neural networks to model sustained elbow flexion: one trained solely on recorded data (i.e., direct learning) and one pre-trained on simulated data and fine-tuned on recorded data (i.e., transfer learning). We simulated muscle activations and joint torques using a musculoskeletal model and a muscle fatigue model (n = 1,701 simulations). We also recorded static subject-specific features (e.g., anthropometric measurements) and dynamic muscle activations and torques during sustained elbow flexion in healthy young adults (n = 25 subjects). Using the simulated dataset, we pre-trained a long short-term memory neural network (LSTM) to regress fatiguing elbow flexion torque from muscle activations. We concatenated this pre-trained LSTM with a feedforward architecture, and fine-tuned the model on recorded muscle activations and static features to predict elbow flexion torques. We trained a similar architecture solely on the recorded data and compared each neural network's predictions on 5 leave-out subjects' data. The transfer learning model outperformed the direct learning model, as indicated by a decrease of 24.9% in their root-mean-square-errors (6.22 Nm and 8.28 Nm, respectively). The transfer learning model and direct learning model outperformed analogous musculoskeletal simulations, which consistently underpredicted elbow flexion torque. Our results suggest that transfer learning from simulated to recorded datasets can decrease reliance on assumptions inherent to biomechanical models and yield predictions robust to real-world conditions.

This study aimed to systematically review and summarise the evidence about the effect of muscle fatigue on the knee proprioception of trained and non-trained individuals. A search in PubMed, Scopus, Web of Science and EBSCO databases and Google Scholar was conducted using the expression: "fatigue" AND ("proprioception" OR "position sense" OR "repositioning" OR "kinesthesia" OR "detection of passive motion" OR "force sense" OR "sense of resistance") AND "knee". Forty-two studies were included. Regarding joint-position sense, higher repositioning errors were reported after local and general protocols. Kinesthesia seems to be more affected when fatigue is induced locally, and force sense when assessed at higher target forces and after eccentric protocols. Muscle fatigue, both induced locally or generally, has a negative impact on the knee proprioception.

Muscle fatigue significantly impacts coordination, stability, and speed in daily activities. Accurate assessment of muscle fatigue is vital for effective exercise programs, injury prevention, and sports performance enhancement. Current methods mostly focus on individual muscles and strength evaluation, overlooking overall fatigue in multi-muscle movements. This study introduces a comprehensive muscle fatigue model using non-negative matrix factorization (NMF) weighting. NMF is employed to analyze the duration multi-muscle weight coefficient matrix (DMWCM) during synergistic movements, and four electromyographic (EMG) signal features in time, frequency, and complexity domains are selected. Particle Swarm Optimization (PSO) optimizes feature weights. The DMWCM and weighted features combine to calculate the Comprehensive Muscle Fatigue Index (CMFI) for multi-muscle synergistic movements. Experimental results show that CMFI correlates with perceived exertion (RPE) and Speed Dynamic Score (SDS), confirming its accuracy and real-time tracking in assessing multi-muscle synergistic movements. This model offers a more comprehensive approach to muscle fatigue assessment, with potential benefits for exercise training, injury prevention, and sports medicine.

Recently, fuzzy dispersion entropy (DispEn) has attracted much attention as a new nonlinear dynamics method that combines the advantages of both DispEn and fuzzy entropy. However, it suffers from limitation of insensitivity to dynamic changes. To solve this limitation, we proposed fractional fuzzy dispersion entropy (FFDispEn) based on DispEn, a novel fuzzy membership function and fractional calculus. The fuzzy membership function was defined based on the Euclidean distance between the embedding vector and dispersion pattern. Simulated signals generated by the one-dimensional (1D) logistic map were used to test the sensitivity of the proposed method to dynamic changes. Moreover, 29 subjects were recruited for an upper limb muscle fatigue experiment, during which surface electromyography (sEMG) signals of the biceps brachii muscle were recorded. Both simulated signals and sEMG signals were processed using a sliding window approach. Sample entropy (SampEn), DispEn and FFDispEn were separately used to calculate the complexity of each frame. The sensitivity of different algorithms to the muscle fatigue process was analyzed using fitting parameters through linear fitting of the complexity of each frame signal. The results showed that for simulated signals, the larger the fractional order q , the higher the sensitivity to dynamic changes. Moreover, DispEn performed poorly in the sensitivity to dynamic changes compared with FFDispEn. As for muscle fatigue detection, the FFDispEn value showed a clear declining tendency with a mean slope of -1.658 × 10 -3 as muscle fatigue progresses; additionally, it was more sensitive to muscle fatigue compared with SampEn (slope: -0.4156 × 10 -3 ) and DispEn (slope: -0.1675 × 10 -3 ). The highest accuracy of 97.5% was achieved with the FFDispEn and support vector machine (SVM). This study provided a new useful nonlinear dynamic indicator for sEMG signal processing and muscle fatigue analysis. The proposed method may be useful for physiological and biomedical signal analysis.

Muscle length changes may evoke alternating activity and consequently reduce local fatigue and pain during prolonged static bending. The aim of this study was to assess whether a postural intervention involving intermittent trunk extensor muscle length changes (INTERMITTENT) can delay muscle fatigue during prolonged static bending when compared to a near-isometric condition (ISOMETRIC) or when participants were allowed to voluntarily vary muscle length (VOLUNTARY). These three conditions were completed by 11 healthy fit male participants, in three separate sessions of standing with 30 ± 3 degrees trunk inclination until exhaustion. Conventional and high-density electromyography (convEMG and HDsEMG, respectively) were measured on the left and right side of the spine, respectively. The endurance time for INTERMITTENT was 33.6% greater than ISOMETRIC (95% CI: [3.8, 63.5]; p = 0.027) and 29.4% greater than VOLUNTARY (95% CI: [7.0, 51.7]; p = 0.010), but not different between ISOMETRIC and VOLUNTARY. The convEMG and HDsEMG amplitude coefficient of variation was significantly greater for INTERMITTENT versus ISOMETRIC. The rate of change in convEMG and HDsEMG spectral content did not reveal significant differences between conditions as found in endurance time. Additional regression analyses between endurance time and rate of change in convEMG (p > 0.05) and HDsEMG (R 2  = 0.39-0.65, p = 0.005-0.039) spectral content indicated that HDsEMG better reflects fatigue development in low-level contractions. In conclusion, imposed intermittent trunk extensor muscle length changes delayed muscle fatigue development when compared to a near-isometric condition or when participants were allowed to voluntarily vary muscle length, possibly due to evoking alternating activity between/within trunk extensor muscles., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Hypoxia is known to increase muscle fatigue via both central and peripheral mechanisms. Females are typically less fatigable than males during isometric fatiguing contractions due to greater peripheral blood flow. However, sex differences in fatigue are blunted during dynamic fatiguing tasks. Thus, this study determined the interactions of sex and hypoxia on knee extensor muscle contractile function during a dynamic, ischemic fatiguing contraction. Electrical stimulation was used to determine contractile properties of the knee extensor muscles in eight males and eight females before and after an ischemic, dynamic fatiguing task while inspiring room air or a hypoxic gas mixture (10% O 2 :90% N 2 ). Fatigue (assessed as time-to-task failure) was ∼10% greater during the hypoxic condition (94.3 ± 33.4 s) compared with normoxic condition (107.0 ± 42.8 s, P = 0.041) and ∼40% greater for females than males (77.1 ± 18.8 vs. 124.2 ± 38.7, P < 0.001). Immediately after the dynamic fatiguing task, there were reductions in maximal voluntary contraction force ( P = 0.034) and electrically evoked twitch force ( P < 0.001), and these reductions did not differ based on sex or inspirate. Cerebral tissue oxygenation showed a significant interaction of time and inspirate ( P = 0.003) whereby it increased during normoxia and remained unchanged in hypoxia. No sex-related differences in the changes of cerebral tissue oxygenation were observed ( P = 0.528). These data suggest that acute hypoxia increases central fatigue during ischemic single-leg exercise resulting in earlier exercise termination, but the effect does not differ based on sex. NEW & NOTEWORTHY Hypoxia exacerbates fatigue via central mechanisms after ischemic single-leg exercise. The greater fatigue observed during ischemic dynamic fatiguing exercise with hypoxia inspirate did not differ between the sexes. Hypoxia-induced central limitations are present in acute ischemic exercise and do not appear different in males and females.

Background: Many people work in a sitting position where they have to use their upper extremities and hands. Muscle activity in sitting position is affected by the chair height of, the height of the worktable, and the distance to the working target., Objective: The purpose of this study is to investigate differences in muscle activity between men and wemen at different chair heghts and working distances., Methods: Surface electromyography (EMG) was employed to determine the muscle activity of the upper limbs and the trunk, while an arm-stretching task was performed in a sitting position., Results: Women's muscle activity was higher than that of men in most muscles as the chair height and working distance changed., Conclusions: As the height of the chair or the working distance was changed, muscle activity was greater in women than in men for most of the muscles analyzed. Under the same conditions, women may feel more muscle fatigue than men, so it is necessary to consider gender in the working environment.

Myoelectric indices forecasting is important for muscle fatigue monitoring in wearable technologies, adaptive control of assistive devices like exoskeletons and prostheses, functional electrical stimulation (FES)-based Neuroprostheses, and more. Non-stationary temporal development of these indices in dynamic contractions makes forecasting difficult. This study aims at incorporating transfer learning into a deep learning model, Myoelectric Fatigue Forecasting Network (MEFFNet), to forecast myoelectric indices of fatigue (both time and frequency domain) obtained during voluntary and FES-induced dynamic contractions in healthy and post-stroke subjects respectively. Different state-of-the-art deep learning models along with the novel MEFFNet architecture were tested on myoelectric indices of fatigue obtained during [Formula: see text] voluntary elbow flexion and extension with four different weights (1 kg, 2 kg, 3 kg, and 4 kg) in sixteen healthy subjects, and [Formula: see text] FES-induced elbow flexion in sixteen healthy and seventeen post-stroke subjects under three different stimulation patterns (customized rectangular, trapezoidal, and muscle synergy-based). A version of MEFFNet, named as pretrained MEFFNet, was trained on a dataset of sixty thousand synthetic time series to transfer its learning on real time series of myoelectric indices of fatigue. The pretrained MEFFNet could forecast up to 22.62 seconds, 60 timesteps, in future with a mean absolute percentage error of 15.99 ± 6.48% in voluntary and 11.93 ± 4.77% in FES-induced contractions, outperforming the MEFFNet and other models under consideration. The results suggest combining the proposed model with wearable technology, prosthetics, robotics, stimulation devices, etc. to improve performance. Transfer learning in time series forecasting has potential to improve wearable sensor predictions.

Purpose: Hot water immersion (HWI) has gained popularity to promote muscle recovery, despite limited data on the optimal heat dose. The purpose of this study was to compare the responses of two exogenous heat strains on core body temperature, hemodynamic adjustments, and key functional markers of muscle recovery following exercise-induced muscle damage (EIMD)., Methods: Twenty-eight physically active males completed an individually tailored EIMD protocol immediately followed by one of the following recovery interventions: HWI (40°C, HWI 40 ), HWI (41°C, HWI 41 ) or warm water immersion (36°C, CON 36 ). Gastrointestinal temperature (T gi ), hemodynamic adjustments (cardiac output [CO], mean arterial pressure [MAP], and systemic vascular resistance [SVR]), pre-frontal cortex deoxyhemoglobin (HHb), ECG-derived respiratory frequency, and subjective perceptual measures were tracked throughout immersion. In addition, functional markers of muscle fatigue (maximal concentric peak torque [T peak ]) and muscle damage (late-phase rate of force development [RFD 100-200 ]) were measured prior to EIMD (pre-), 24 h (post-24 h), and 48 h (post-48 h) post-EIMD., Results: By the end of immersion, HWI 41 led to significantly higher T gi values than HWI 40 (38.8 ± 0.1 vs. 38.0°C ± 0.6°C, p < 0.001). While MAP was well maintained throughout immersion, only HWI 41 led to increased (HHb) (+4.2 ± 1.47 μM; p = 0.005) and respiratory frequency (+4.0 ± 1.21 breath.min -1 ; p = 0.032). Only HWI 41 mitigated the decline in RFD 100-200 at post-24 h (-7.1 ± 31.8%; p = 0.63) and T peak at post-48 h (-3.1 ± 4.3%, p = 1)., Conclusion: In physically active males, maintaining a core body temperature of ~25 min within the range of 38.5°C-39°C has been found to be effective in improving muscle recovery, while minimizing the risk of excessive physiological heat strain., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Although the underlying mechanisms behind upper limb (e.g., finger) motor slowing during movements performed at the maximum voluntary rate have been explored, the same cannot be said for the lower limb. This is especially relevant considering the lower limb's larger joints and different functional patterns. Despite the similar motor control base, previously found differences in movement patterns and segment inertia may lead to distinct central and peripheral manifestations of fatigue in larger joint movement. Therefore, we aimed to explore these manifestations in a fatiguing knee maximum movement rate task by measuring brain and muscle activity, as well as brain-muscle coupling using corticomuscular coherence, during this task. A significant decrease in knee movement rate up to half the task duration was observed. After an early peak, brain activity showed a generalized decrease during the first half of the task, followed by a plateau, whereas knee flexor muscle activity showed a continuous decline. A similar decline was also seen in corticomuscular coherence but for both flexor and extensor muscles. The electrophysiological manifestations associated with knee motor slowing therefore showed some common and some distinct aspects compared with smaller joint tasks. Both central and peripheral manifestations of fatigue were observed; the changes seen in both EEG and electromyographic (EMG) variables suggest that multiple mechanisms were involved in exercise regulation and fatigue development. NEW & NOTEWORTHY The loss of knee movement rate with acute fatigue induced by high-speed movement is associated with both central and peripheral electrophysiological changes, such as a decrease in EEG power, increased agonist-antagonist cocontraction, and impaired brain-muscle coupling. These findings had not previously been reported for the knee joint, which shows functional and physiological differences compared with the existing findings for smaller upper limb joints.

Surface electromyogram (EMG) signals find diverse applications in movement rehabilitation and human-computer interfacing. For instance, future advanced prostheses, which use artificial intelligence, will require EMG signals recorded from several sites on the forearm. This requirement will entail complex wiring and data handling. We present the design and evaluation of a bespoke EMG sensing system that addresses the above challenges, enables distributed signal processing, and balances local versus global power consumption. Additionally, the proposed EMG system enables the recording and simultaneous analysis of skin-sensor impedance, needed to ensure signal fidelity. We evaluated the proposed sensing system in three experiments, namely, monitoring muscle fatigue, real-time skin-sensor impedance measurement, and control of a myoelectric computer interface. The proposed system offers comparable signal acquisition characteristics to that achieved by a clinically-approved product. It will serve and integrate future myoelectric technology better via enabling distributed machine learning and improving the signal transmission efficiency.

The aim of the present study was to assess the effect of beetroot juice supplementation (BEET) on neuromuscular fatigue etiology during simulated soccer match play. In a randomized, double-blind, crossover design, 13 soccer players completed the Loughborough Intermittent Shuttle Test (LIST). Players received either BEET (2×150 mL; ∼8 mmol/L nitrate) or placebo (PLA) for 7 days (6 days prior to the experimental session and on the day of trial, 2 h before LIST). Neuromuscular assessments were performed at baseline, 45 min (half time: HT), and 90 min (full time: FT) following LIST. Maximal voluntary contraction (MVC) and twitch responses, delivered through electrical femoral nerve stimulation, were used to assess peripheral (quadriceps resting twitch force Q tw,pot ) and central fatigue (voluntary activation, VA). Compared with baseline, MVC Q tw,pot and VA values decreased in PLA and BEET conditions at HT and FT ( P  < 0.05). Compared with PLA, the decrease in MVC and Q tw,pot was significantly attenuated with BEET at HT and FT ( P  < 0.001). Likewise, BEET attenuated the decrease in VA at HT ( P  < 0.001, d  = 1.3) and FT ( P  < 0.001, d  = 1.5) compared with the PLA condition. Chronic beetroot juice supplementation attenuates neuromuscular fatigue development during simulated soccer matches, and this is due to both central and peripheral factors. Consequently, chronic beetroot may optimize physical performance., Competing Interests: The authors have no conflict of interest to report.

Previous studies have identified some sex differences in how individual muscles change their activation during repetitive multi-joint arm motion-induced fatigue. However, little is known about how indicators of multi-muscle coordination change with fatigue in males and females. Fifty-six (29 females) asymptomatic young adults performed a repetitive, forward-backward pointing task until scoring 8/10 on a Borg CR10 scale while surface electromyographic activity of upper trapezius, anterior deltoid, biceps brachii, and triceps brachii was recorded. Activation coefficient, synergy structure, and relative weight of each muscle within synergies were calculated using the non-negative matrix factorization method. Two muscle synergies were extracted from the fatiguing task. The synergy structures were mostly preserved after fatigue, while the activation coefficients were altered. A significant Sex × Fatigue interaction effect showed more use of the anterior deltoid in males especially before fatigue in synergy 1 during shoulder stabilization (p = 0.04). As for synergy 2, it was characterized by variations in the relative weight of biceps, which was higher by 16 % in females compared to males (p = 0.04), and increased with fatigue (p = 0.03) during the elbow flexion acceleration phase and the deceleration phase of the backward pointing movement. Findings suggest that both sexes adapted to fatigue similarly, using fixed synergy structures, with alterations in synergy activation patterns and relative weights of individual muscles. Results support previous findings of an important role for the biceps and anterior deltoid in explaining sex differences in patterns of repetitive motion-induced upper limb fatigue., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The absolute magnitude and rate of arterial desaturation each independently impair whole-body aerobic exercise. This study examined potential mechanisms underlying the rate-dependent relationship. Utilizing an exercise protocol involving unilateral, intermittent, isometric knee extensions (UIIKE), we provided sufficient reperfusion time between contractions to reduce the accumulation of intramuscular metabolic by-products that typically stimulate muscle afferents. The objective was to create a milieu conducive to accentuating any influence of arterial desaturation rate on muscular fatigue. Eight participants completed four UIIKE sessions, performing one 3 s contraction every 30s at a perceived intensity of 50% MVC for 25 min. Participants voluntarily adjusted their force generation to maintain perceptual effort at 50% MVC without feedback. Reductions in inspired oxygen fraction (F I O 2 ) decreased arterial saturation from >98% to 70% with varying rates in three trials: FAST (5.3 ± 1.3 min), MED (11.8 ± 2.7 min), and SLOW (19.9 ± 3.7 min). F I O 2 remained at 0.21 during the control trial. Force generation and muscle activation remained at baseline levels throughout UIIKE trials, unaffected by the magnitude or rate of desaturation. Minute ventilation increased with hypoxia (p < 0.05), and faster desaturation rates magnified this response. These findings demonstrate that arterial desaturation magnitude and rate independently affect ventilation, but do not influence fatigue development during UIIKE., (© 2024 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)

Introduction: This case study aimed to describe potential changes in neuromuscular activation and synergies after an 8-day cross-country mountain-bike stage race., Methods: A peak power output test was performed 5 days before the race. Two days before the start and after 7 days of racing, the athlete performed a power-based Lamberts Submaximal Cycling Test, including surface electromyography, and completed a Daily Analysis of Life Demands of Athletes questionnaire. Neuromuscular activation, in terms of root mean square, timing (onset-offset) of muscle activation, and synergies, was obtained from electromyography recordings., Results: The athlete reported an increase in symptoms of experienced stress after the stage race on the Daily Analysis of Life Demands of Athletes questionnaire. Both biceps femoris and tibialis anterior muscles showed a reduction in normalized amplitude after the stage race. In addition, the number of synergies that was necessary to describe neuromuscular activation increased from 2 to 3., Conclusions: In this case study, the increase in synergies suggests that, after the stage race, the athlete showed a more complex muscle-recruitment pattern. This may indicate that muscle coordination can change when muscle fatigue occurs; however, further research is needed to confirm these results in a larger sample.

Muscle fatigue has proven to be a main factor in developing work-related musculoskeletal disorders. Taking small breaks or performing stretching routines during a work shift might reduce workers' fatigue. Therefore, our objective was to explore how breaks and/or a stretching routine during a work shift could impact muscle fatigue and body kinematics that might subsequently impact the risk of work-related musculoskeletal disorder (WMSD) risk during material handling jobs. We investigated muscle fatigue during a repetitive task performed without breaks, with breaks, and with a stretching routine during breaks. Muscle fatigue was detected using muscle activity (electromyography) and a validated kinematic score measured by wearable sensors. We observed a significant reduction in muscle fatigue between the different work-rest schedules ( p < 0.01). Also, no significant difference was observed between the productivity of the three schedules. Based on these objective kinematic assessments, we concluded that taking small breaks during a work shift can significantly reduce muscle fatigue and potentially reduce its consequent risk of work-related musculoskeletal disorders without negatively affecting productivity.

The purpose of this study was to investigate the effect of inducing post-activation potentiation (PAP) during prolonged low-frequency force depression (PLFFD) on motor unit (MU) firing rates. In 10 participants, grouped firing rates of 3027 MUs from the tibialis anterior were recorded with tungsten microelectrodes. Baseline MU firing rates at 25% isometric maximal voluntary contraction (MVC) were ∼14 Hz. A 1 min dorsiflexion MVC reduced torque and maximal MU firing rates (36 Hz) by 49% and 52%, respectively. Following task completion, firing rates at 25% of baseline MVC torque and torque in response to electrically evoked (single twitch, 10 Hz and 50 Hz) stimulation were assessed before and after a 5 s MVC (to induce PAP) every 10 min for 60 min. From 10 to 60 min after task completion, the torque ratios (twitch:50 Hz and 10:50 Hz) were depressed (∼30%) relative to baseline (P < 0.001), indicating PLFFD; and firing rates were higher by ∼15% relative to baseline (P < 0.001). This occurred despite recovery of MVC rates (∼99%) and torque (∼95%) by 10 min (P > 0.3). Inducing PAP during PLFFD increased both low to high torque ratios (twitch and 10:50 Hz) by ∼200% and ∼135%, respectively (P < 0.001) and firing rates were ∼18% lower relative to PLFFD rates (P < 0.001), despite a speeding of evoked contractile properties (P = 0.001). Thus, firing rates appear strongly matched to alterations in torque, rather than contractile speed when modified by contractile history, and lower rates during PAP may be a mechanism to mitigate effects of PLFFD. The effect of activation history on contractile function demonstrates acute compensatory responses of motoneuron output. KEY POINTS: Prolonged low frequency force depression (PLFFD) following a sustained 1 min isometric maximal voluntary contraction causes an increase in submaximal mean motor unit (MU) firing rates. Inducing post-activation potentiation (PAP) during PLFFD, however, causes a reduction in mean submaximal MU firing rates to a level below those at baseline. The mean firing rate reduction during PAP occurs despite a speeding of evoked contractile properties and thus firing rates are more strongly matched to alterations in torque, rather than contractile speed when modified by various contractile histories. The reductions in firing rates during PAP may mitigate the effects of PLFFD during voluntary contractions. These results demonstrate that firing rates are highly responsive to opposing influences on the contractile state and can make rapid compensatory rate adjustments dependent on the active state of the muscle., (© 2023 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.)

Previous studies have shown that the dominant arm is generally stronger and more resistant to fatigue. However, whether there are side differences in shoulder muscle activation during a fatiguing upper limb task, and whether this varies according to sex, is unknown. Thirty right-handed adults (15 females) were recruited to complete two sessions of an overhead repetitive fatiguing task (shoulder flexion between 90 and 135° at 1 Hz), performed in two separate sessions with their dominant arm (DA) and non-dominant arm (NDA) until exhaustion. Electromyographic (EMG) data was collected from 11 shoulder muscles of the moving arm, and their activation amplitude (RMS) and activation variability (SD) were assessed. Results show that time to exhaustion was not affected by arm or by sex. There were some main arm effects on EMG activity amplitude, with higher activity on the DA's pectoralis major (p < 0.001), and on the NDA's middle (p = 0.009) and posterior deltoid (p = 0.001) and infraspinatus (p < 0.001). The pectoralis major was affected by arm and fatigue mostly in males. Their DA's pectoralis major activity amplitude was higher, and the amplitude variability was lower, compared to the NDA, with both parameters showing fatigue-dependent decreases at the NDA only (arm x sex x fatigue: RMS: p = 0.007; SD: p = 0.001). As for females, the DA variability of their lower trapezius was smaller, and that of their subscapularis was higher, compared to the NDA (sex x arm, p = 0.028, p = 0.05). There was also more EMG variability on the supraspinatus' dominant side, and on the posterior deltoid and infraspinatus ND side. Results show an overhead shoulder flexion task dependency on pectoralis major control in males, and on lower trapezius and shoulder girdle stabilizers in females, which could be related to both sex- and gender-based factors. This knowledge can help identify side-specific injury risk factors due to overhead work in males and females, and help determine the appropriateness of implementing sex-specific workplace protocols, including alternating arms as fatigue compensatory and recovery strategies., Competing Interests: Declaration of Competing Interest None., (Copyright © 2023 Elsevier B.V. All rights reserved.)

This study aims to identify how spatial distribution of lumbar muscle activity is modulated by different fatigue tasks. Twenty healthy adults performed two different isometric trunk extension endurance tasks (the modified Sorensen test and the inverted modified Sorensen test) until exhaustion. During these tasks, bilateral superficial lumbar muscle activity was recorded using high-density electromyography. The spatial distribution of activation within these muscles was obtained using the centroid coordinates in the medio-lateral and cranio-caudal directions. The effects of task and endurance time (left and right sides) were investigated using repeated measures ANOVA. Results revealed a significant lateral shift of the centroid throughout the fatigue tasks on both sides and no difference between tasks. Significant task × time interaction effects were found for the cranio-caudal direction on both sides showing a significantly more caudal location of the centroid in the modified Sorensen test compared to the inverted test at the beginning of the tasks. Our findings suggest that spatial distribution of lumbar muscle activity is task-dependent in a pre-fatigue stage while an alternative but similar muscle recruitment strategy is used in both tasks to maintain performance in the later stages of muscle fatigue., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

A reduced muscle glycogen content and potassium (K + ) disturbances across muscle membranes occur concomitantly during repeated intense exercise and together may contribute to skeletal muscle fatigue. Therefore, we examined whether raised extracellular K + concentration ([K + ] o ) (4 to 11 mM) interacts with lowered glycogen to reduce force production. Isometric contractions were evoked in isolated mouse soleus muscles (37°C) using direct supramaximal field stimulation. (1) Glycogen declined markedly in non-fatigued muscle with >2 h exposure in glucose-free physiological saline compared with control solutions (11 mM glucose), i.e. to <45% control. (2) Severe glycogen depletion was associated with increased 5'-AMP-activated protein kinase activity, indicative of metabolic stress. (3) The decline of peak tetanic force at 11 mM [K + ] o was exacerbated from 67% initial at normal glycogen to 22% initial at lowered glycogen. This was due to a higher percentage of inexcitable fibres (71% vs. 43%), yet without greater sarcolemmal depolarisation or smaller amplitude action potentials. (4) Returning glucose while at 11 mM [K + ] o increased both glycogen and force. (5) Exposure to 4 mM [K + ] o glucose-free solutions (15 min) did not increase fatiguability during repeated tetani; however, after recovery there was a greater force decline at 11 mM [K + ] o at lower than normal glycogen. (6) An important exponential relationship was established between relative peak tetanic force at 11 mM [K + ] o and muscle glycogen content. These findings provide direct evidence of a synergistic interaction between raised [K + ] o and lowered muscle glycogen as the latter shifts the peak tetanic force-resting E M relationship towards more negative resting E M due to lowered sarcolemmal excitability, which hence may contribute to muscle fatigue. KEY POINTS: Diminished muscle glycogen levels and raised extracellular potassium concentrations ([K + ] o ) occur simultaneously during intense exercise and together may contribute to muscle fatigue. Prolonged exposure of isolated non-fatigued soleus muscles of mice to glucose-free physiological saline solutions markedly lowered muscle glycogen levels, as does fatigue then recovery in glucose-free solutions. For both approaches, the subsequent decline of maximal force at 11 mM [K + ] o , which mimics interstitial [K + ] levels during intense exercise, was exacerbated at lowered compared with normal glycogen. This was mainly due to many more muscle fibres becoming inexcitable. We established an important relationship that provides evidence of a synergistic interaction between raised [K + ] o and lowered glycogen content to reduce force production. This paper indicates that partially lowered muscle glycogen (and/or metabolic stress) together with elevated interstitial [K + ] interactively lowers muscle force, and hence may diminish performance especially during repeated high-intensity exercise., (© 2023 The Authors. The Journal of Physiology © 2023 The Physiological Society.)

This study aimed to investigate whether acute passive heat stress 1) decreases muscle Maximal Voluntary Contraction (MVC); 2) increases peripheral muscle fatigue; 3) increases spinal cord excitability, and 4) increases key skeletal muscle gene signaling pathways in skeletal muscle. Examining the biological and physiological markers underlying passive heat stress will assist us in understanding the potential therapeutic benefits. MVCs, muscle fatigue, spinal cord excitability, and gene signaling were examined after control or whole body heat stress in an environmental chamber (heat; 82 °C, 10% humidity for 30 min). Heart Rate (HR), an indicator of stress response, was correlated to muscle fatigue in the heat group (R = 0.59; p < 0.05) but was not correlated to MVC, twitch potentiation, and H reflex suppression. Sixty-one genes were differentially expressed after heat (41 genes >1.5-fold induced; 20 < 0.667 fold repressed). A strong correlation emerged between the session type (control or heat) and principal components (PC1) (R = 0.82; p < 0.005). Cell Signal Transduction, Metabolism, Gene Expression and Transcription, Immune System, DNA Repair, and Metabolism of Proteins were pathway domains with the largest number of genes regulated after acute whole body heat stress. Acute whole-body heat stress may offer a physiological stimulus for people with a limited capacity to exercise., Competing Interests: Declaration of competing interest The authors report no conflicts of interest., (Copyright © 2023. Published by Elsevier Ltd.)

Augmented tactile and performance feedback has been used to (re)train a modified lifting technique to reduce lumbar spine flexion, which has been associated with low back disorder development during occupational repetitive lifting tasks. However, it remains unknown if the presence of trunk extensor neuromuscular fatigue influences learning of this modified lifting technique. Therefore, we compared the effectiveness of using augmented tactile and performance feedback to reduce lumbar spine flexion during a repetitive lifting task, in both unfatigued and fatigued states. Participants completed repetitive lifting tests immediately before and after training, and 1-week later, with half of the participants completing training after fatiguing their trunk extensor muscles. Both groups demonstrated learning of the modified lifting technique as demonstrated by increased thorax-pelvis coordination variability and reduced lumbar range of motion variability; however, experiencing trunk extensor neuromuscular fatigue during lift (re)training may have slight negative influences on learning the modified lifting technique. Practitioner summary: An augmented lift (re)training paradigm using tactile cueing and performance feedback regarding key movement features (i.e. lumbar spine flexion) can effectively (re)train a modified lifting technique to reduce lumbar flexion and redistribute motion to the hips and knees. However, performing (re)training while fatigued could slightly hinder learning this lifting technique.

ABSTRACT Females demonstrate greater fatigue resistance compared to males in tasks ranging from single-limb contractions to whole-body exercise, including running. Many of the studies investigating sex differences in fatigability following running, however, occur after long duration, low-intensity tasks and it is unknown whether there is a sex difference in fatigability following high-intensity running. This study compared fatigability and recovery following a 5 km running time trial in young males and females. Sixteen recreationally active participants (8 males, 8 females, age: 23 ± 4 years) completed a familiarisation and experimental trial. Knee-extensor maximal voluntary contractions (MVCs) were performed before and up to 30 min after a 5 km time trial on a treadmill. Heart rate and rating of perceived exertion (RPE) were recorded after every kilometre during the time trial. Although not significantly different, males completed the 5 km time trial 15% faster than females ( p =  0.095). Heart rate ( p  = 0.843) and RPE ( p  = 0.784) were similar between the sexes during the trial. Prior to running, males had larger MVCs ( p  = 0.014). The relative decrease in MVC force was less in females than males immediately post-exercise (-4.6 ± 2.4% vs. -15.1 ± 3.0%, p  < 0.001) and at 10-minutes post-exercise ( p  = 0.018). At 20- and 30-minutes recovery, however, relative MVC force was not different between the sexes ( p  ≥ 0.129). These data demonstrate that females experienced less fatigability of the knee extensors than males following a high-intensity 5 km running time trial. The findings highlight the need to understand responses to exercise in both sexes and have implications for recovery from training and exercise prescription. Highlights Data regarding sex differences in fatigability following high-intensity running is relatively sparse.Therefore, this study quantified the decrease in knee-extensor maximum voluntary contraction force (MVC) following a 5-km self-paced running time trial.Despite similar heart rates and ratings of perceived exertion, the percentage decrease in MVC was three times greater in males compared to females.Relative MVCs remained greater in females compared to males until 20 min post-exercise.

The study evaluated the reliability and repeatability of the force and surface electromyography activity (EMG) outcomes obtained through voluntary and electrically evoked contractions of knee extensors in females ( n = 18) and males ( n = 20) and compared these data between sexes. Maximal isometric voluntary contractions (iMVCs) of knee extensors associated with electrical stimulation of the femoral nerve were performed over 4 days (48-h interval), with the first day involving familiarization procedures, the second involving three trials (1-h interval), and the third and fourth involving just one trial. The intraclass correlation coefficient (ICC), coefficient of variation (CV), and repeatability of outcomes from within- and between-day trials were determined for each sex. Females presented lower maximal voluntary force during iMVC (iMVC Force ) and associated vastus lateralis EMG activity (root mean square, RMS VL ), force evoked by potentiated doublet high-frequency (Db 100Force ) and single stimuli ( Q tw ), and M-wave amplitude than males ( P ≤ 0.01, partial eta squared ≥0.94). Voluntary activation (VA) and RMS VL /M-wave amplitude did not differ between sexes. iMVC Force , VA, Db 100Force , Q tw , and M-wave amplitude were the most reliable outcomes in within-day trials, with similar results between sexes (ICC > 0.62; CV < 6.4%; repeatability: 12.2%-22.6%). When investigating between-day trials, the iMVC Force , VA, Db 100Force , and Q tw were the most reliable (ICC > 0.66; CV < 7.5%; repeatability: 13.2%-33.45%) with similar results between sexes. In conclusion, females presented lower iMVC Force and evoked response than males. Although reliability and repeatability statistics vary between trials, data (e.g., from EMG or force signal), and sexes, most of the outcomes obtained through this technique are reliable in females and males. NEW & NOTEWORTHY Although reliability and repeatability of knee extensors vary according to the type of neuromuscular function outcome (e.g., from force or EMG responses), the trial intervals (i.e., hours or days), and the sex of the participant, most force and EMG outcomes obtained through these neuromuscular assessment protocols present ICC > 0.75, very good CV (<10%), and repeatability <25% in within- and between-day trials in both sexes.

Trunk extensor muscle fatigue typically manifests as a decline in spectral content of surface electromyography. However, previous research on the relationship of this decline with trunk extensor muscle endurance have shown inconsistent results. The decline of spectral content mainly reflects the decrease in average motor unit action potential conduction velocity (CV). We evaluated whether the rate of change in CV, as well as two approaches employing the change in spectral content, are related to trunk extensor muscle endurance. Fourteen healthy male participants without a low-back pain history performed a non-strictly controlled static forward trunk bending trial until exhaustion while standing. For 13 participants, physiologically plausible CV estimates were obtained from high-density surface electromyography bilaterally from T6 to L5. Laterally between L1 and L2, the linear rate of CV change was strongly correlated to endurance time (R 2  = 0.79), whereas analyses involving the linear rate of change in spectral measures showed a lower (R 2  = 0.38) or no correlation. For medial electrode locations, estimating CV and its relationship with endurance time was less successful, while the linear rate of change in spectral measures correlated moderately to endurance time (R 2  = 0.44; R 2  = 0.56). This study provides guidance on monitoring trunk extensor muscle fatigue development using electromyography., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Critical torque (CT) represents the highest oxidative steady state for intermittent knee extensor exercise, but the extent to which it is influenced by skeletal muscle mitochondria and sex is unclear. Vastus lateralis muscle biopsy samples were collected from 12 females and 12 males -matched for relative maximal oxygen uptake normalized to fat-free mass (FFM) (F: 57.3 (7.5) ml (kg FFM) -1  min -1 ; M: 56.8 (7.6) ml (kg FFM) -1  min -1 ; P = 0.856) - prior to CT determination and performance fatiguability trials. Males had a lower proportion of myosin heavy chain (MHC) I isoform (40.6 (18.4)%) compared to females (59.5 (18.9)%; P = 0.021), but MHC IIa and IIx isoform distributions and protein markers of mitochondrial content were not different between sexes (P > 0.05). When normalized to maximum voluntary contraction (MVC), the relative CT (F: 42.9 (8.3)%; M: 37.9 (9.0)%; P = 0.172) and curvature constant, W' (F: 26.6 (11.0) N m s (N m) -1 ; M: 26.4 (6.5) N m s (N m) -1 ; P = 0.962) were not significantly different between sexes. All protein biomarkers of skeletal muscle mitochondrial content, as well as the proportion of MHC I isoform, positively correlated with relative CT (0.48 < r < 0.70; P < 0.05), and the proportion of MHC IIx isoform correlated positively with relative W' (r = 0.57; P = 0.007). Indices of performance fatiguability were not different between males and females for MVC- and CT-controlled trials (P > 0.05). Greater mitochondrial protein abundance was associated with attenuated declines in potentiated twitch torque for exercise at 60% MVC (P < 0.05); however, the influence of mitochondrial protein abundance on performance fatiguability was reduced when exercise was prescribed relative to CT. Whether these findings translate to whole-body exercise requires additional research. KEY POINTS: The quadriceps critical torque represents the highest intensity of intermittent knee extensor exercise for which an oxidative steady state is attainable, but its relationship with skeletal muscle mitochondrial protein abundance is unknown. Matching males and females for maximal oxygen uptake relative to fat-free mass facilitates investigations of sex differences in exercise physiology, but studies that have compared critical torque and performance fatiguability during intermittent knee extensor exercise have not ensured equal aerobic fitness between sexes. Skeletal muscle mitochondrial protein abundance was correlated with critical torque and fatigue resistance for exercise prescribed relative to maximum voluntary contraction but not for exercise performed relative to the critical torque. Differences between sexes in critical torque, skeletal muscle mitochondrial protein abundance and performance fatiguability were not statistically significant. Our results suggest that skeletal muscle mitochondrial protein abundance may contribute to fatigue resistance by influencing the critical intensity of exercise., (© 2023 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.)

Objective: Muscle health and decreased muscle performance (fatigue) quantification has proven to be an invaluable tool for both athletic performance assessment and injury prevention. However, existing methods estimating muscle fatigue are infeasible for everyday use. Wearable technologies are feasible for everyday use and can enable discovery of digital biomarkers of muscle fatigue. Unfortunately, the current state-of-the-art wearable systems for muscle fatigue tracking suffer from either low specificity or poor usability., Methods: We propose using dual-frequency bioimpedance analysis (DFBIA) to non-invasively assess intramuscular fluid dynamics and thereby muscle fatigue. A wearable DFBIA system was developed to measure leg muscle fatigue of 11 individuals during a 13-day protocol consisting of exercise and unsupervised at-home portions., Results: We derived a digital biomarker of muscle fatigue, fatigue score, from the DFBIA signals that was able to estimate the percent reduction in muscle force during exercise with repeated-measures Pearson's r = 0.90 and mean absolute error (MAE) of 3.6%. This fatigue score also estimated delayed onset muscle soreness with repeated-measures Pearson's r = 0.83 and MAE = 0.83. Using at-home data, DFBIA was strongly associated with absolute muscle force of participants (n = 198, p < 0.001)., Conclusion: These results demonstrate the utility of wearable DFBIA for non-invasively estimating muscle force and pain through the changes in intramuscular fluid dynamics., Significance: The presented approach may inform development of future wearable systems for quantifying muscle health and provide a novel framework for athletic performance optimization and injury prevention.

Our aim was to compare three electrical stimulation protocols (P20, P30 and P40), with the same number of stimuli, but different stimulation frequencies (20, 30 and 40 Hz, respectively) and duty cycles [1.2:1.2 s (continuous), 0.8:1.2 s (intermittent) and 0.6:1.2 s (intermittent), respectively). Twitch force and the peak-to-peak M-wave amplitude of the thenar muscles were measured before, during and after each protocol at 1-40 Hz in random order. Twelve healthy adults (23-41 years old) were examined for each protocol in random order and in separate sessions. P20 elicited the highest mean force, and P40 the lowest decrease in percent force at the end of the protocol. Force evoked at 1 and 10 Hz decreased less after P40, compared with P20 and P30. The M-wave amplitude was significantly reduced throughout all protocols, with the largest decrease observed during P30. Although an increase in frequency typically induced earlier and greater decrement in force, this was compensated or even reversed by increasing the interval between each stimulation train, while keeping the number of pulses per stimulation cycle constant. The lesser decrease in M-wave amplitude during P40 compared with P20 indicates that longer between-train intervals may help maintaining the integrity of neuromuscular propagation., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This study investigated the effects of back muscle fatigue on the estimation of low-back loads and active low-back moments during lifting, using an EMG and kinematics based model calibrated with data from an unfatigued state. Fourteen participants performed lifting tasks in unfatigued and fatigued states. Fatigue was induced through semi-static forward bending. EMG, kinematics, and ground reaction forces were measured, and low-back loads were estimated using inverse dynamics and EMG-driven muscle model. A regression model was developed using data from a set of calibration lifts, and its accuracy was evaluated for unfatigued and fatigued lifts. During the fatigue-inducing task, the EMG amplitude increased by 2.8 %MVC, representing a 38% increase relative to the initial value. However, during the fatigued lifts, the peak EMG amplitude was found to be 1.6 %MVC higher than that observed during the unfatigued lifts, representing a mere 4% increase relative to the baseline unfatigued peak EMG amplitude. Kinematics and low-back load estimates remained unaffected. Regression model estimation errors remained unaffected for 5 kg lifts, but increased by no more than 5% of the peak active low-back moment for 15 kg lifts. We conclude that the regression-based estimation quality of active low-back moments can be maintained during periods of muscle fatigue, although errors may slightly increase for heavier loads., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Low-frequency fatigue (LFF) is usually defined as the decline in low:high-frequency force of electrically evoked isometric muscle contractions. The influence of LFF on dynamic muscle function is not well studied. Our aim was to assess the effect of LFF on the electrically evoked torque-velocity relationship in humans. Sixteen participants underwent a series of electrically evoked knee extensions in an isokinetic dynamometer to establish torque-velocity relationships at 15 and 50 Hz using isokinetic contractions. Hereafter, fatigue was induced by five sets of 10 repetitions of maximal voluntary dynamic knee extensions. After 30 min of rest, torque-velocity tests were repeated. Maximal torque (F max ) was measured, whereas maximal contraction velocity (V max ) and maximal power (P max ) were estimated using Hill's force-velocity equation, 15:50 Hz ratios were calculated for F max , V max , and P max as markers of LFF. F max decreased by 40% at 15 Hz ( P = 0.001) and by 15% at 50 Hz ( P = 0.001) in the fatigued state. No significant change was detected for V max at 15 Hz [-2%, ( P = 0.349)] or 50 Hz [+3% ( P = 0.763)], whereas 15 and 50 Hz P max decreased by 30% ( P = 0.004) and 10% ( P = 0.008), respectively. Following the fatigue protocol, the 15:50 Hz F max ratio decreased by 31% ( P < 0.001), indicating LFF. The 15:50 Hz P max ratio also decreased by 23% ( P = 0.002), whereas the 15:50 Hz V max ratio was unchanged ( P = 0.313). In conclusion, fatiguing contractions decreased F max and P max at both high and low stimulation frequencies, whereas V max appeared unaffected. Nevertheless, LFF influences power production during human dynamic contractions at a range of submaximal velocities. NEW & NOTEWORTHY Force-velocity relationships were established using either low- or high-frequency electrical stimulation before and after fatiguing voluntary eccentric/concentric contractions of the knee extensors. Low-frequency fatigue was assessed by the relative decrease in low- and high-frequency maximal torque, maximal shortening velocity, and maximal power estimated by the force-velocity relationship. Low-frequency fatigue manifests itself as a large decrease in low-frequency maximal force and power with a modest decrease in high-frequency maximal force and power. Contraction velocity does not seem to decrease in the same manner.