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Rhodolitica occulta gen. nov. et sp. nov. (Clavulariidae) is described from Cocos Island National Park, Pacific Ocean, Costa Rica. The species was found at various islets and rocky outcrops around the island, 20-55 m in depth. The genus is characterised by tubular, single, erect anthosteles interconnected by thin basal ribbon-like stolons on the surfaces of living rhodoliths. The anthosteles are devoid of fused sclerites, which are only present in the stolons. Coenenchymal sclerites are mostly spindles of various shapes, with a characteristic cylindrical warty type in the outer layer, crosses and radiates. Anthocodiae are armed with points, lacking collarets. Colonies and sclerites are red. Using an integrative taxonomic approach, we separate the new genus from similar genera through both morphological comparison and a molecular phylogenetic analysis. This research is a contribution to the knowledge of the octocoral biodiversity in Cocos Island and marine biodiversity in the eastern tropical Pacific. [ABSTRACT FROM AUTHOR]

Eutrophication is one of the causes of the degradation of reefs worldwide. The aim of this research is to determine if sewage discharge reaches the fore reefs at northwest of Cuba using δ15N in tissues of the octocorals Eunicea flexuosa and Plexaura kuekenthali and the concentration of microbiological and physical-chemical variables. Thirteen reefs at 10-m depth were selected near river basins and far from the urban and industrial development of Havana City. Branch tips of both species were collected, the concentrations of nutrient and microorganisms in water samples were quantified, and horizontal visibility in the water (Vis) was determined. Overall, δ15N of E. flexuosa ranged from 1.5 to 6.3‰ and P. kuekenthali from 1.7 to 6.7‰. The tissue of both species was significantly enriched in 15N in reefs near polluted watersheds compared with reefs far from pollution by anthropogenic activities. The δ15N of both species showed a positive and significant correlation with the concentration of fecal and total coliform bacteria, heterotrophic bacteria, and NH4+ and a negative and significant correlation with the Vis. The δ15N of the two species and microbiological and physical-chemical variables evidenced water quality decline by sewage discharge that reached reefs near polluted watersheds. [ABSTRACT FROM AUTHOR]

In the present study, prokaryotic communities from nine sponge species, two octocoral species, sediment and seawater were assessed from Maldivian coral reefs. All host species contained prokaryotic communities, which were distinct from those found in sediment and seawater. Certain host species, however, contained relatively high abundances of environmental OTUs, that is, OTUs recorded in sediment and/or seawater. This was particularly the case for the sponge species Svenzea novo sp. and Stylissa carteri with respect to seawater and Liosina paradoxa with respect to sediment. The sponge species Svenzea novo sp., S. carteri, L. paradoxa, Jaspis splendens and Ptilocaulis cf. spiculifer all had relatively high abundances of Proteobacteria, Cyanobacteria and Planctomycetes and relatively low evenness compared to the remaining sponge species. They also housed distinct subsets of highly abundant OTUs, which were rare or absent in other hosts, a trait they shared with the octocoral species. The aforementioned compositional traits suggest LMA status for these sponge species. The remaining species (Hyrtios erectus, Luffariella variabilis, Neopetrosia chaliniformis and Plakortis cf. kenyensis) had high relative abundances of Chloroflexi, Actinobacteria, Acidobacteria, Gemmatimonadetes, PAUC34f (or SAUL; sponge‐associated unclassified lineage) and Poribacteria, high evenness and limited compositional variation among samples, traits associated with HMA status. Our results, furthermore, confirmed the previously established classification of H. erectus as an HMA and S. carteri as a LMA species. Both octocoral species (Melithaea maldivensis and Paraminabea sp.) housed prokaryotic communities, which were compositionally distinct from sponge species and included significantly discriminating OTUs assigned to the Tenericutes, Spirochaetae and Proteobacteria phyla and the genera Mycoplasma (Tenericutes), Spirochaeta 2 (Spirochaetae) and Endozoicomonas (Proteobacteria). [ABSTRACT FROM AUTHOR]

Highly abundant and widespread populations of the introduced eastern Pacific ophiuroid brittle star Opthiothela mirabilis occur in southeast Florida, extending the range of this new non-native species from southern Brazil northward to the Caribbean Sea and Florida, now spanning about 6000 km of latitude along western Atlantic tropical/subtropical shores. This brittle star species, representing two lineages, is an epizoite on shallow-occurring (3–15 m depth) plexaurid octocorals (e.g., Eunicea spp., Muricea elongata, and Plexaurella dichotoma). Overall body color (disk and arms) is commonly beige or orange; a few individuals display combinations of both colors: orange disk/beige arms or beige disk/orange arms. Population densities in situ commonly ranged between 5 and 12 individuals of O. mirabilis per 10-cm-long octocoral branch with up to six arms generally curled around their octocoral host's branches. In a 33-day experiment, M. elongata colony surface areas with reduced (contracted) polyps and reduced polyp extension lengths were not statistically different between octocorals with brittle stars versus octocorals without brittle stars. At the end of 33 days, brittle star densities demonstrated an overall decline. In a 66-day experiment, increasing brittle star densities resulted in reduced extended polyp areas in M. elongata, but no changes in Eunicea flexuosa. Polyp extension lengths decreased in both octocoral hosts with increasing densities of O. mirabilis. Overall, experimental brittle star densities did not change, with several octocoral hosts revealing increases in O. mirabilis by means of fissiparity. The presence and increasing densities of O. mirabilis causing a reduction in octocoral polyp extension were greatest in M. elongata but did not result in visible adverse effects to host condition. Black spotting on octocoral stems, a sign of tissue deterioration, was not observed during or after the two experiments. Nearly all experimental octocorals demonstrated tissue growth in the 66-day experiment, expanding over relatively large areas of the supporting pedestals. [ABSTRACT FROM AUTHOR]

In an effort to facilitate virus isolation-based studies on coral reefs, we describe here a simple holobiont virus extraction protocol that is effective at separating and concentrating virus particles from coral reef invertebrates. We demonstrate the application of this protocol by isolating and barcoding cyanophages from invertebrate holobionts associated with coral reefs in southwest Puerto Rico. Cyanophages were also isolated and barcoded from adjacent coral reef seawater. Barcoding of cyanophage isolates was carried out with the cyanomyovirus DNA polymerase (g43) or major capsid protein (g23) marker genes. We then utilized cyanophage sequences from Puerto Rico, along with those published previously associated with Rhode Island and Bermuda seawater, to assess the presence of cyanophage-like sequences in reef invertebrate virome, metagenome, and transcriptome sequencing libraries. The detailed holobiont virus extraction protocol successfully separated and concentrated virus particles from the tissue of 20 different species of coral reef invertebrate. Cyanophages were isolated and barcoded from 15 of these species: three scleractinian corals, a gorgonian, a corallimorpharian, a zoantharian, two hydrozoans, four species of sponges, two tunicates, and a nudibranch. In total, there were 146 cyanophages isolated and barcoded from seawater (n = 46) or invertebrate tissue (n = 100). The majority (69%) of cyanomyovirus sequences reported in this study were novel, sharing rather low similarity (< 98% nucleotide similarity) with publicly available sequences in the NCBI nucleotide database. Sequence library mining efforts revealed evidence of cyanophage-like sequences in 23 next-generation sequencing datasets, representing 17 species of coral reef invertebrates which included seven species of stony corals, one scyphozoan, seven species of sponges, and two species of copepods. This is the first investigation into cyanophage diversity on Puerto Rico reefs and is a relevant step in coral reef virology, ideally stimulating holobiont-associated isolation efforts to further explore virus genetic and functional diversity within invertebrate tissue on the reef. [ABSTRACT FROM AUTHOR]

Recent shifts in the presence and abundance of species on shallow Caribbean coral reefs have left octocorals as the dominant functional group on some reefs, creating an 'animal forest' with an associated canopy. This transition changes the reef profile potentially affecting flow and sedimentation. We examined the effects of an octocoral forest on the depositional environment on a shallow-water fringing reef system on the south shore of St. John, USVI. The depositional environment was characterized as canopy or non-canopy based on octocoral density. The effect of the octocoral canopy on flow and sedimentation was assessed using clod cards and sediment traps at 15 paired locations. The octocoral canopy altered flow resulting in greater levels of turbulence within the canopy. Sediment traps in areas of dense octocoral canopy accumulated greater amounts of sediment, with coarser, more rounded grains. Organic content of sediments collected in the traps was greater within the canopy than outside of it. The increase in turbulence within the canopy was likely due to wave-driven oscillatory flow interacting with the octocoral colonies. Sediment traps in the canopy likely had greater sediment accumulation due to both resuspension of sediments from within the canopy and deposition of imported sediments as flow decreased within the canopy. The presence of octocoral canopies and the reworking of sediment within them may affect the success of settling larvae and the evolution of reef structure. [ABSTRACT FROM AUTHOR]

The MeOH extract of the Vietnamese octocoral Dendronephthya mucronata afforded four bicyclic lactones (1–4), including three new compounds namely dendronephthyones A–C (1–3), after subjecting it on various chromatographic separations. The structures of the isolated compounds were established by spectroscopic experiments including 1D, 2D NMR, CD and HR-QTOF-MS. In addition, compounds 1–4 were found to exhibit selective cytotoxicity against the HeLa human cancer cell line with IC50 values of 32.48 ± 2.15, 30.12 ± 1.86, 35.14 ± 1.57 and 14.45 ± 1.34 μM, respectively. [ABSTRACT FROM AUTHOR]

In the current context of climate change, benthic cnidarians of the genus Palythoa have been suggested to be resistant owing to their intrinsic biological characteristics. In tropical regions, some species are currently proliferating in areas where environmental conditions are less suitable for other organisms, even replacing hard coral ecosystems.Considering their tropical affinities, phase‐shifts towards Palythoa‐dominated areas could become more frequent in future climate change scenarios, leading to changes in ecosystem organization. The aim of this study was to evaluate the effect of climate change stressors in two common Palythoa spp. with different habitat affinities within a subtropical region, and the effect upon their predator–prey interactions.The results of this experimental study demonstrated that colonies of P. aff. clavata and P. caribaeorum were significantly affected by exposure to temperature and pH conditions predicted for 2100 in the Canary Islands, during 62 days.Despite zoantharians' lack of carbonate in their body wall, Palythoa spp. were most affected in their growth rates by lowered pH, and colonies significantly decreased in weight and size. Although all colonies exhibited symptoms of bleaching at high temperature, a reduction in chlorophyll content was also observed at low pH.Predation by Platypodiella picta crabs decreased on P. aff. clavata exposed to acidic conditions, which may compensate for the lowered ecological performance of the species in these climate change conditions. In contrast, P. picta was able to actively feed on P. caribaeorum colonies regardless of the experimental conditions.Despite being suggested as winner species in a climate change scenario, our study demonstrated that low pH negatively impacted Palythoa spp. survival. If the species are not able to acclimatize to the new conditions, changes in their populations may be expected, although their magnitude could be ameliorated by means of a decrease in predation rates. [ABSTRACT FROM AUTHOR]

Background: In octocorals (Cnidaria Octocorallia), the functional relationship between host health and its symbiotic consortium has yet to be determined. Here, we employed comparative metagenomics to uncover the distinct functional and phylogenetic features of the microbiomes of healthy Eunicella gazella, Eunicella verrucosa, and Leptogorgia sarmentosa tissues, in contrast with the microbiomes found in seawater and sediments. We further explored how the octocoral microbiome shifts to a pathobiome state in E. gazella. Results: Multivariate analyses based on 16S rRNA genes, Clusters of Orthologous Groups of proteins (COGs), Protein families (Pfams), and secondary metabolite-biosynthetic gene clusters annotated from 20 Illumina-sequenced metagenomes each revealed separate clustering of the prokaryotic communities of healthy tissue samples of the three octocoral species from those of necrotic E. gazella tissue and surrounding environments. While the healthy octocoral microbiome was distinguished by so-far uncultivated Endozoicomonadaceae, Oceanospirillales, and Alteromonadales phylotypes in all host species, a pronounced increase of Flavobacteriaceae and Alphaproteobacteria, originating from seawater, was observed in necrotic E. gazella tissue. Increased abundances of eukaryotic-like proteins, exonucleases, restriction endonucleases, CRISPR/Cas proteins, and genes encoding for heat-shock proteins, inorganic ion transport, and iron storage distinguished the prokaryotic communities of healthy octocoral tissue regardless of the host species. An increase of arginase and nitric oxide reductase genes, observed in necrotic E. gazella tissues, suggests the existence of a mechanism for suppression of nitrite oxide production by which octocoral pathogens may overcome the host's immune system. Conclusions: This is the first study to employ primer-less, shotgun metagenome sequencing to unveil the taxonomic, functional, and secondary metabolism features of prokaryotic communities in octocorals. Our analyses reveal that the octocoral microbiome is distinct from those of the environmental surroundings, is host genus (but not species) specific, and undergoes large, complex structural changes in the transition to the dysbiotic state. Host-symbiont recognition, abiotic-stress response, micronutrient acquisition, and an antiviral defense arsenal comprising multiple restriction endonucleases, CRISPR/Cas systems, and phage lysogenization regulators are signatures of prokaryotic communities in octocorals. We argue that these features collectively contribute to the stabilization of symbiosis in the octocoral holobiont and constitute beneficial traits that can guide future studies on coral reef conservation and microbiome therapy. CGE-K3mkWRXvLuipSC6CFk Video Abstract [ABSTRACT FROM AUTHOR]

—During 79th cruise of R/V Akademik Mstislav Keldysh twelve trawl hauls were collected in Powell Basin and adjacent area at depths 370–3771 m. The structure of the benthic assemblages was highly variable when considered at the macrotaxon level. The main factors shaping the taxonomical structure of the sampled assemblages were depth, near-bottom current velocities and proportion of the hard fraction of the sediment. Hard substrate assemblages of the Antarctic peninsula slope and Philip Ridge with high percentage of filter-feeders (mainly sponges) were associated with the highest values of the near-bottom tide currents (up to 55.6 cm/s). In several trawl hauls indicator species of vulnerable marine ecosystems—mainly Demospongiae sponges, deep-sea corals Stylasteridae (Hydrozoa) and Primnoidae (Octocorallia)—were collected. [ABSTRACT FROM AUTHOR]

Coral reefs are known to have extremely high rates of primary production. However, common geochemical methods for determining bulk rates of reef metabolism cannot distinguish which organisms are responsible for primary production. Here we used a "bottom-up" approach to estimate the contribution of diverse primary producers including hard corals, octocorals, and algae to gross primary production on coral reefs by scaling up taxon-specific rates by the abundance of those taxa in the environment. Chamber-based production rates of the dominant primary producers were obtained as a function of irradiance, the primary short-term driver of photosynthesis. These rates were then combined with annotated three-dimensional (3D) reconstructions of reef sections and a simple light field model to estimate reef-scale gross and net primary production rates over time. At a degraded reef in the Florida Keys octocorals and algae were the main producers, but at a more intact site a scleractinian coral (Acropora palmata) was the most important producer. As a validation of the approach, rates of primary production estimated using the "bottom-up" approach were compared with in situ eddy covariance fluxes. The daily integrated rates agreed within 16%, though maximal production was ~ 35% lower in the "bottom- up" approach likely due to under-representation of octocorals and macroalgae in the 3D reconstructions. The "bottom up" approach yielded results that were largely consistent with the in situ measurements of primary production and irradiance with the significant benefit of providing taxon-specific and spatially-explicit primary production rates. [ABSTRACT FROM AUTHOR]

Carijoa riisei is a snowflake coral that has aggressively spread across many coastal habitats in the Atlantic and Pacific oceans, threatening a number of tropical ecosystems worldwide, including coral reefs. The aim of this work was to evaluate the distribution and provide an estimation of abundance of the invasive octocoral C. riisei along the Ecuadorian coast, as well as the relationship between its abundance and different environmental variables. In a field survey, high abundances of C. riisei colonies were reported growing over corals and sessile communities at 5 of 43 sampling sites. The areas with highest relative abundance were found in the Manabí province, at two sites in Jama: Bajo Londres (44.57% coverage) and Vaca Brava 1 (20.25%). Results of ordination and grouping statistical analyses showed no significant differences between invaded and not invaded sites as regards community composition or environmental characteristics, suggesting neither biotic nor abiotic factors could be limiting C. riisei dispersal along the Ecuadorian coast. Results from a bibliographic survey covering occurrence data up to 2020 were in accordance, showing that in that period C. riisei became an established species to the Ecuadorian coast, being present in at least 22 of the 43 sites, including various sites in Marine Protected Areas. Based on these findings, recommendations are made to promote urgent monitoring programs to detect C. riisei in new areas along the coast of Ecuador and in the Galapagos Marine Reserve, in order to develop a mitigation program and to take actions to conserve the ecosystems affected by this invasion. [ABSTRACT FROM AUTHOR]

The recently described deep-sea pennatulacean genus Porcupinella was previously known only by the type species, Porcupinella profunda from the equatorial eastern Atlantic to the eastern North Atlantic Ocean. New data is provided on morphology, distribution, bathymetry, and related taxa. A second species is added here as well -- a new species is described from the Tasman Sea in the southwestern Pacific. The new species, Porcupinella tasmanica, is distinguished from P. profunda by its distinctive hook-shaped growth form, laterally compressed dorsal keel, and differing regions that are occupied by siphonozooids. A key to the species of the deep-sea pennatulacean family Chunellidae is included based on comparative morphology. [ABSTRACT FROM AUTHOR]

Three new sea fan species of Leptogorgia were discovered during multiple scuba diving expeditions along the Gulf of California coast and islands. Leptogorgia iridis sp. nov. is distributed in the southern region of the gulf (Mexican Province), inhabiting tropical rocky reefs of the Islas Marías Archipelago (Nayarit) and Bahía Banderas (Jalisco). This species has small colonies (< 7 cm height) with at least five clearly distinct chromotypes. Leptogorgia martirensis sp. nov. was found exclusively on the rocky reefs of San Pedro Mártir and San Esteban Islands located in the northern region of the Gulf of California (northern region of Cortez Province). Leptogorgia enrici sp. nov. is distributed from the south to the northern region of the Gulf of California (Cortez Province), inhabiting substrates of rocky reefs, sandy and pebbly sea floors. Comprehensive ecological diving expeditions to identify and classify octocorals in the Mexican Pacific (1995-2019) indicate that L. iridis sp. nov. and L. martirensis sp. nov. are likely to be micro-endemics and L. enrici sp. nov. is endemic to the Gulf of California, which defines their currently known biogeographic distribution ranges. [ABSTRACT FROM AUTHOR]

Anthozoans exhibit great plasticity in their responses to stressful conditions, including decreasing individual size, detaching from the substratum and relocating, and releasing endosymbiotic microalgae. Another response to stress used by some colonial anthozoans is polyp bailout, in which the coenenchyme breaks down and individual polyps detach from the colony. We observed polyp bailout in the common Caribbean gorgonian Eunicea flexuosa after 8 h of aerial exposure. After 9 days, 28% of bailed-out polyps reattached, although none opened to resume feeding. Polyp bailout is a costly and high-risk escape response, but reattachment indicates that this can be a genet-saving behavior in cases where whole-colony mortality is likely. While it has been described in two octocorals, several species of scleractinians, and one black coral, we still do not know how widespread this behavior is in anthozoans. [ABSTRACT FROM AUTHOR]

Successful recruitment is critical to the maintenance and resilience of populations and may be at the core of the transition from scleractinian- to octocoral-dominated faunas on some Caribbean reefs. For sessile invertebrates, recruitment incorporates the composite effects of larval supply, settlement and survival. The relative success of these processes differs between species, and successful recruitment may be achieved through different life history strategies. Recruitment of six abundant and widespread Antillogorgia spp. was assessed at six sites on Little Bahama Bank from 2009–2012. Identification of recruits to species level, based on microsatellite analyses, revealed differences in recruitment and survival between species, sites and years. The broadcast spawning species, A. americana and A. acerosa, had low rates of early recruitment and post-settlement survival. Higher levels of recruitment success were achieved among brooding and surface brooding species following somewhat different patterns of early recruitment and survival. The internal brooder A. hystrix had the highest recruitment at five of the sites, but low survival dramatically reduced its abundance and after a year it had similar densities as the surface brooding species, A. elisabethae and A. bipinnata. The brooders have smaller colonies and produce fewer larvae than the broadcast spawning species, but they release competent larvae, which may account for their higher recruitment rates. The Antillogorgia illustrate the diversity of successful reproductive strategies exhibited by octocorals, and differences in the life history strategies among these congeners are best characterized by their mode of larval development. [ABSTRACT FROM AUTHOR]

Octocorals (Octocorallia, Alcyonacea) are distributed worldwide and their taxonomy in the Persian Gulf has been a subject of controversy among experts due to their cryptic features. Here, we present a primary review of the Alcyonacea in the Iranian Persian Gulf with molecular documentation to provide insight into the real diversity within this order. Our molecular study was based on two mitochondrial protein-coding (COI and mtMuts) and one nuclear (28S rDNA) gene sequences, which identified seven families, 14 genera, and 21 species of Octocorallia in the Persian Gulf that were well-supported by maximum likelihood and Bayesian analyses. Our results indicate consistency between morphological and molecular characteristics at the species level. We conclude the presence of some heterogeneous groups of genera creates ambiguity in our analysis. [ABSTRACT FROM AUTHOR]

Corallium rubrum has been exploited by humankind for centuries. The long-term exploitation dynamics of this species make it even more important today to increase protection and restoration efforts as it provides a significant range of ecosystem services. This becomes even more important in areas where natural recovery is hindered or unlikely. So far, only very few experiments have been carried out in the past, investigating suitable techniques for the successful transplantation of this species. For this reason, a review was conducted in order to synthesize previous results and identify the most promising methodologies. Additionally, six different transplantation techniques were tested and discussed in the context of the review. Five techniques used fragments for transplantation, while one used newly settled larvae on PVC-tiles. Shallow C. rubrum colonies often grow upside down under crevices and rims as well as in caves, making the transplantation of fragments comparatively challenging. Here, C. rubrum was transplanted upside down under crevices using a PVC-grid in combination with epoxy putty to hold fragments in place, and the results indicated the potential benefits of this technique. In a novel approach, shallow colonies, and larvae on settling plates were also transferred to deeper areas, suggesting that mesophotic populations can be restored to reconstruct pre-exploitation conditions. Attaching the colonies to the roof of crevices provided a level of survivorship consistent with conventional erect transplantations of colonies on rock bottom but had the advantage of being more removed from sedimentation and anthropogenic disturbance. Future work must develop permanent grid-mounting methods for use in the crevices before this approach can be further explored for large-scale restoration efforts.

More branches on severely clipped colonies (reduced to four branches; 72.4%) underwent positive growth compared to colonies that were clipped to 10 branches (65.3%) or not clipped (62.6%). The differences among colonies in total number of mother branches had only a small effect on the overall number of new branches generated on each mother branch (corrected Figure 4). Moderately clipped colonies from Abaco (clipped to 10 branches) had more new branches per mother branch in comparison to moderately clipped colonies from San Salvador and to severely clipped colonies at both sites (which had similar numbers of new branches per source branch; corrected Figure 3B). [Extracted from the article]

The interplay among environment, demography, and host-parasite interactions is a challenging frontier. In the ocean, fundamental changes are occurring due to anthropogenic pressures, including increased disease outbreaks on coral reefs. These outbreaks include multiple parasites, calling into question how host immunity functions in this complex milieu. Our work investigates the interplay of factors influencing co-infection in the Caribbean sea fan octocoral, Gorgonia ventalina , using metrics of the innate immune response: cellular immunity and expression of candidate immune genes. We used existing copepod infections and live pathogen inoculation with the Aspergillus sydowii fungus, detecting increased expression of the immune recognition gene Tachylectin 5A (T5A) in response to both parasites. Cellular immunity increased by 8.16% in copepod infections compared to controls and single Aspergillus infections. We also detected activation of cellular immunity in reef populations, with a 13.6% increase during copepod infections. Cellular immunity was similar in the field and in the lab, increasing with copepod infections and not the fungus. Amoebocyte density and the expression of T5A and a matrix metalloproteinase (MMP) gene were also positively correlated across all treatments and colonies, irrespective of parasitic infection. We then assessed the scaling of immune metrics to population-level disease patterns and found random co-occurrence of copepods and fungus across 15 reefs in Puerto Rico. The results suggest immune activation by parasites may not alter parasite co-occurrence if factors other than immunity prevail in structuring parasite infection. We assessed non-immune factors in the field and found that sea fan colony size predicted infection by the copepod parasite. Moreover, the effect of infection on immunity was small relative to that of site differences and live coral cover, and similar to the effect of reproductive status. While additional immune data would shed light on the extent of this pattern, ecological factors may play a larger role than immunity in controlling parasite patterns in the wild. Parsing the effects of immunity and ecological factors in octocoral co-infection shows how disease depends on more than one host and one parasite and explores the application of co-infection research to a colonial marine organism. [ABSTRACT FROM AUTHOR]

Background: The process of calcium carbonate biomineralization has arisen multiple times during metazoan evolution. In the phylum Cnidaria, biomineralization has mostly been studied in the subclass Hexacorallia (i.e. stony corals) in comparison to the subclass Octocorallia (i.e. red corals); the two diverged approximately 600 million years ago. The precious Mediterranean red coral, Corallium rubrum, is an octocorallian species, which produces two distinct highmagnesium calcite biominerals, the axial skeleton and the sclerites. In order to gain insight into the red coral biomineralization process and cnidarian biomineralization evolution, we studied the protein repertoire forming the organic matrix (OM) of its two biominerals. Results: We combined High-Resolution Mass Spectrometry and transcriptome analysis to study the OM composition of the axial skeleton and the sclerites. We identified a total of 102 OM proteins, 52 are found in the two red coral biominerals with scleritin being the most abundant protein in each fraction. Contrary to reef building corals, the red coral organic matrix possesses a large number of collagen-like proteins. Agrin-like glycoproteins and proteins with sugar-binding domains are also predominant. Twenty-seven and 23 proteins were uniquely assigned to the axial skeleton and the sclerites, respectively. The inferred regulatory function of these OM proteins suggests that the difference between the two biominerals is due to the modeling of the matrix network, rather than the presence of specific structural components. At least one OM component could have been horizontally transferred from prokaryotes early during Octocorallia evolution. Conclusion: Our results suggest that calcification of the red coral axial skeleton likely represents a secondary calcification of an ancestral gorgonian horny axis. In addition, the comparison with stony coral skeletomes highlighted the low proportion of similar proteins between the biomineral OMs of hexacorallian and octocorallian corals, suggesting an independent acquisition of calcification in anthozoans. [ABSTRACT FROM AUTHOR]

As coral populations decline worldwide in the face of ongoing environmental change, documenting their distribution, diversity and conservation status is now more imperative than ever. Accurate delimitation and identification of species is a critical first step. This task, however, is not trivial as morphological variation and slowly evolving molecular markers confound species identification. New approaches to species delimitation in corals are needed to overcome these challenges. Here, we test whether target enrichment of ultraconserved elements (UCEs) and exons can be used for delimiting species boundaries and population structure within species of corals by focusing on two octocoral genera, Alcyonium and Sinularia, as exemplary case studies. We designed an updated bait set (29,181 baits) to target‐capture 3,023 UCE and exon loci, recovering a mean of 1,910 ± 168 SD per sample with a mean length of 1,055 ± 208 bp. Similar numbers of loci were recovered from Sinularia (1,946 ± 227 SD) and Alcyonium (1,863 ± 177 SD). Species‐level phylogenies were highly supported for both genera. Clustering methods based on filtered single nucleotide polymorphisms delimited species and populations that are congruent with previous allozyme, DNA barcoding, reproductive and ecological data for Alcyonium, and offered further evidence of hybridization among species. For Sinularia, results were congruent with those obtained from a previous study using restriction site associated DNA sequencing. Both case studies demonstrate the utility of target‐enrichment of UCEs and exons to address a wide range of evolutionary and taxonomic questions across deep to shallow timescales in corals. [ABSTRACT FROM AUTHOR]

Background: Whole mitogenomes or short fragments (i.e., 300–700 bp of the cox1 gene) are the markers of choice for revealing within- and among-species genealogies. Protocols for sequencing and assembling mitogenomes include 'primer walking' or 'long PCR' followed by Sanger sequencing or Illumina short-read low-coverage whole genome (LC-WGS) sequencing with or without prior enrichment of mitochondrial DNA. The aforementioned strategies assemble complete and accurate mitochondrial genomes but are time consuming and/or expensive. In this study, I first tested whether mitogenomes can be sequenced from long-read nanopore sequencing data exclusively. Second, I explored the accuracy of the long-read assembled genomes by comparing them to a 'gold' standard reference mitogenome retrieved from the same individual using Illumina sequencing. Third and lastly, I tested if the long-read assemblies are useful for mitophylogenomics and barcoding research. To accomplish these goals, I used the Caribbean spiny lobster Panulirus argus, an ecologically relevant species in shallow water coral reefs and target of the most lucrative fishery in the greater Caribbean region. Results: LC-WGS using a MinION ONT device and various de-novo and reference-based assembly pipelines retrieved a complete and highly accurate mitogenome for the Caribbean spiny lobster Panulirus argus. Discordance between each of the long-read assemblies and the reference mitogenome was mostly due to indels at the flanks of homopolymer regions. Although not 'perfect', phylogenetic analyses using entire mitogenomes or a fragment of the cox1 gene demonstrated that mitogenomes assembled using long reads reliably identify the sequenced specimen as belonging to P. argus and distinguish it from other related species in the same genus, family, and superorder. Conclusions: This study serves as a proof-of-concept for the future implementation of in-situ surveillance protocols using the MinION to detect mislabeling in P. argus across its supply chain. Mislabeling detection will improve fishery management in this overexploited lobster. This study will additionally aid in decreasing costs for exploring meta-population connectivity in the Caribbean spiny lobster and will aid with the transfer of genomics technology to low-income countries. [ABSTRACT FROM AUTHOR]

Since all forms of mimicry are based on perceptual deception, the sensory ecology of the intended receiver is of paramount importance to test the necessary precondition for mimicry to occur, that is, model‐mimic misidentification, and to gain insight in the origin and evolutionary trajectory of the signals. Here we test the potential for aggressive mimicry by a group of coral reef fishes, the color polymorphic Hypoplectrus hamlets, from the point of view of their most common prey, small epibenthic gobies and mysid shrimp. We build visual models based on the visual pigments and spatial resolution of the prey, the underwater light spectrum and color reflectances of putative models and their hamlet mimics. Our results are consistent with one mimic‐model relationship between the butter hamlet H. unicolor and its model the butterflyfish Chaetodon capistratus but do not support a second proposed mimic‐model pair between the black hamlet H. nigricans and the dusky damselfish Stegastes adustus. We discuss our results in the context of color morphs divergence in the Hypoplectrus species radiation and suggest that aggressive mimicry in H. unicolor might have originated in the context of protective (Batesian) mimicry by the hamlet from its fish predators rather than aggressive mimicry driven by its prey. [ABSTRACT FROM AUTHOR]

Degradation of marine and coastal ecosystem can be seen everywhere and Goa is no exception. Biodiversity provide important services to mankind and needs to be protected. Goa is endowed with varied microhabitats along its 105 long coast line. These habitats are home to diverse forms of life about which little is known. The coastal waters serve as nursery to fishes and other animals which are exploited commercially. The present communication reviews the current status on the taxonomic knowledge of the coastal biodiversity of Goa. The coastal water of Goa abound in flora and fauna. The information on major fauna and flora of Goa is sufficiently available. There is, however, some lacunae in our knowledge on invertebrate subgroups, such as sponges, octocorals, ctenophores, and tunicates and minor phyla. The biodiversity of specialized ecosystems continued to be inadequately known and remains a challenge to specialists from Goa coast. Threat to coastal and marine biodiversity due to climate change and environmental degradation are intensifying with time. The anthropogenic activities are causing damage to pristine coastal ecosystems. The loss of biodiversity will be felt in greater strength by future generation. The present knowledge on coastal biodiversity warrants continued taxonomic research in the least-studied or unknown groups of hot spot areas in the light of existing threats to marine biodiversity. It is suggested that further research on new species occurrences in the least studied groups should be continued to update the data and improve our knowledge. The appropriate measures will be required to protect the rich biodiversity resources of Goa. [ABSTRACT FROM AUTHOR]

Cnidarians are known to undergo reverse development as a survival mechanism against adverse environmental conditions. Polyp bail-out consists in the polyps' detachment from the mother colony due to stressful conditions, followed by a complete tissue and cells rearrangement and in some cases in a regression into a simple, ciliated form. Here we describe a massive polyp bail-out event occurred in the mesophotic black coral Antipathella subpinnata in reared conditions. This is the first report of a bail-out event in this species providing new insights into the life cycle and ecology of black corals. [ABSTRACT FROM AUTHOR]

A 6‐year‐old, intact male, brindled, 30‐Lb English Bulldog presented to the Purdue University Veterinary Teaching Hospital with a recurrent history of hematuria, periuria, and lethargy that responded temporarily to antibiotic therapy. The work‐up included a complete blood count, serum biochemical profile, complete urinalysis, diagnostic imaging (abdominal radiographs and ultrasound with contrast urography), and exploratory laparotomy. The diagnostic imaging findings and subsequent exploratory revealed a unilateral, intraluminal, right‐sided, 3‐cm ureteral mass extending from the proximal ureter into the renal pelvis. Subsequently, a unilateral right‐sided ureteronephrectomy followed by biopsy with cytopathology/cytology (impression smears) and histopathology of the ureteral mass was performed. The cytopathologic interpretation was benign mesenchymal proliferation with mildly atypical urothelial cells. The association of this mass with vascular tissue and a benign nuclear appearance on cytology is similar to reports of fibroepithelial polyps (FEPs) and myxomatous tumors. Histopathology diagnosed the mass as an FEP. Cytopathology proved useful in the presumptive diagnosis of this benign urothelial polyp. To the authors' knowledge, this is the first report using cytopathology to depict and characterize FEPs in veterinary and human medical literature. [ABSTRACT FROM AUTHOR]

Explorations of seamounts in the Western Pacific Ocean and South China Sea resulted in collecting 18 specimens of golden gorgonians. Based on the morphology and the genetic analysis of mtMutS, they are described as one new species, Chrysogorgia carolinensis sp. nov., and four known species, including Chrysogorgia dendritica Xu, Zhan & Xu, 2020, Metallogorgia melanotrichos (Wright & Studer, 1889), Metallogorgia macrospina Kükenthal, 1919, and Pseudochrysogorgia bellona Pante & France, 2010. Chrysogorgia carolinensis belongs to the Chrysogorgia "group A, Spiculosae" with rods or spindles distributed in the polyp- body wall and tentacles, and differs from all of its congeners except C. dendritica by the 1/3L branching sequence and amoeba-shaped sclerites at the basal polyp body. The mtMutS sequence of C. carolinensis sp. nov. has six deletion mutations compared to those of its congeners, supporting the establishment of the new species. Although no genetic variability was observed between the closely related species C. dendritica and C. abludo Pante & Watling, 2012, the former is different from the latter by the apparently irregular sclerites in the polyp body wall. The two specimens of Metallogorgia melanotrichos match well with the original description except for relatively larger polyps, while the M. macrospina specimens have slightly smaller polyps than the holotype. The juvenile of Metallogorgia has an obvious morphological difference with the adults in the colony shape and branches, but they can be unified by the same polyps and sclerites as well as mitochondrial MutS sequences. Thus, the generic diagnosis of Metallogorgia is slightly extended to include the morphology of juveniles. The morphology of Pseudochrysogorgia bellona Pante & France, 2010, as a new record for the South China Sea, matches well with that of the original description. In the phylogenetic trees, the Chrysogorgia species are separated into two clades, and while Metallogorgia and Pseudochrysogorgia formed a sister clade. [ABSTRACT FROM AUTHOR]

Background: Pulmonary manifestations are regularly reported in both human and animal filariasis. In human filariasis, the main known lung manifestations are the tropical pulmonary eosinophilia syndrome. Its duration and severity are correlated with the presence of microfilariae. Litomosoides sigmodontis is a filarial parasite residing in the pleural cavity of rodents. This model is widely used to understand the immune mechanisms that are established during infection and for the screening of therapeutic molecules. Some pulmonary manifestations during the patent phase of infection with L. sigmodontis have been described in different rodent hosts more or less permissive to infection. Methods: Here, the permissive Mongolian gerbil (Meriones unguiculatus) was infected with L. sigmodontis. Prevalence and density of microfilariae and adult parasites were evaluated. Lungs were analyzed for pathological signatures using immunohistochemistry and 3D imaging techniques (two-photon and light sheet microscopy). Results: Microfilaremia in gerbils was correlated with parasite load, as amicrofilaremic individuals had fewer parasites in their pleural cavities. Fibrotic polypoid structures were observed on both pleurae of infected gerbils. Polyps were of variable size and developed from the visceral mesothelium over the entire pleura. The larger polyps were vascularized and strongly infiltrated by immune cells such as eosinophils, macrophages or lymphocytes. The formation of these structures was induced by the presence of adult filariae since small and rare polyps were observed before patency, but they were exacerbated by the presence of gravid females and microfilariae. Conclusions: Altogether, these data emphasize the role of host-specific factors in the pathogenesis of filarial infections. [ABSTRACT FROM AUTHOR]

Parasites play significant roles in the function of ecosystems and can make up a large proportion of overall biomass. Yet, fundamental aspects of their ecology are often understudied relative to other organisms. Gnathiid isopods are the primary ectoparasites of fishes in coral reef ecosystems. While some studies have investigated their host-detection capabilities, the means by which they seek hosts are largely unknown. Gnathiids are benthic and live on the bottom, and all collection efforts involving live hosts have thus far involved traps set at or near the reef substrate. We investigated the distance gnathiids will travel vertically in the water column to attach to a fish host. The majority of gnathiids collected were at or less than 1 m above the reef substrate, and gnathiids were collected in serially diminishing numbers at 2 and 3 m above it. No gnathiids were collected from any fish hosts set more than 3.5 m above the reef substrate. Results suggest that gnathiids will actively seek host fishes in situ and will travel further from their benthic habitat than previously known. By swimming into the water column, gnathiids can exploit larger, highly-mobile fishes, which can serve as a major source of dispersal. [ABSTRACT FROM AUTHOR]

• A novel mathematical model is developed. • The model is tested on the test problems. • Interval search ant colony algorithm is developed. • The results are compared with random search, improved ACO and GA • The proposed algorithm outperforms other algorithms. The use of robots is significantly increasing day by day in manufacturing systems, and especially improving the efficiency of the lines. Robots can be used to complete disassembly tasks, and each of the robots can need different operation times to perform the tasks. In this paper, the balancing of the robotic disassembly line problem has been studied to develop efficient solution techniques. Firstly, a mixed-integer linear mathematical model is proposed to determine and solve the problem optimally. A case study from literature is addressed to assess and show the efficiency and effectiveness of the model to minimize cycle time. Secondly, a heuristic algorithm based on ant colony optimization is also proposed to discover a solution for especially the large-size test problems due to the complexity of the problem. The performance of the proposed heuristic algorithm is verified and compared with the different heuristic on data sets. The computational results indicate that the proposed mathematical model and the algorithms are promising for the small and large-size test problems, respectively. Finally, it should be stated that robots have great potential to use in the area of disassembly line and useful solutions provide according to test results. [ABSTRACT FROM AUTHOR]

Covering: 2010 to 2020 The class of 2,11-cyclized cembranoids is particularly widespread in marine Octocorallia, especially Alcyonacea and Gorgonacea, and has been extensively studied. Following a previous review published in 2010, the accumulated knowledge from the past decade will be presented here. All 2,11-cyclized cembranoids share a bicyclo[8.4.0]tetradecane core that is in most of the known compounds oxidatively modified to result in the 15-oxatricyclo[6.6.1.02,7]pentadecane system. Multiple further oxidation and acylation patterns can be observed, while halogenated compounds are surprisingly rare. One new sulfur-containing and a few seco-compounds have also been reported. The biosynthetic pathways to this fascinating class of natural products have not been studied to date, but a plausible biosynthetic hypothesis is presented that allows for a structured discussion of the compounds according to their carbon skeletons and oxidation patterns. Biological activities towards 2,11-cyclized diterpenes are also included. [ABSTRACT FROM AUTHOR]

We present an integrative study with molecular phylogenetic reconstructions and morphological assessment across the three Ceriantharia families: Arachnactidae, Botrucnidiferidae and Cerianthidae. The Arachnactidae specimens (Isarachnanthus spp.) form a well-supported clade, whereas Cerianthidae and Botrucnidiferidae are not recovered as monophyletic. Consequently, the validity of the suborder Spirularia is questioned. Cerianthus was recovered as polyphyletic and Ceriantheomorphe may prove to be a junior synonym of Cerianthus. The taxonomic position of Cerianthus cf. mortenseni is also discussed. All specimens identified on morphology as belonging to Pachycerianthus are recovered as a clade. Further revision of taxa within Ceriantharia is necessary. Molecular phylogenetic analyses based on six mitochondrial or nuclear loci place Ceriantharia as sister to Hexacorallia s.s. , but with no significant support relative to an alternative hypothesis that it is the sister taxon to Octocorallia. Further molecular sequence data and taxon sampling will be needed to resolve the position of Ceriantharia. [ABSTRACT FROM AUTHOR]

Background and Objective: Xenia is an octocoral genus of family Xeniidae. It contains 98 species and is rich of xenican-type diterpenoids. These compounds play an important role in the biological activity of Xenia. Different bioactivities were reported, particularly, anticancer effects. Materials and Methods: A specimen of a Xenia umbellata was exhausted with organic solvents. Then, the organic extract has been fractionated and purified employing different chromatographic procedures. The spectral information obtained from different nuclear magnetic resonance experiments, mass spectroscopy, infrared, and ultraviolet was the key to elucidate the chemical structures. The anti-proliferative activities of all compounds have been evaluated against hepatocellular carcinoma (HepG2), prostate adenocarcinoma (PC-3), and colorectal adenocarcinoma (HT-29) cells. Results: Two new xeniolide-type diterpenes, xeniolide L (1) and xeniolide M (2), along with two known diterpenes, xeniolide K (3) and xeniumbellal (4) were isolated. Compounds 1–4 exhibited significant cytotoxic effect with IC50 values ranged from 0.17 ± 0.01 to 64.7 ± 0.40 μg/mL. Compound 1 displayed late apoptotic and necrotic effects in both HepG2 and PC-3, while 2 exhibited late apoptosis in HepG2 cells. Conclusion: The isolated xeniolide diterpenes displayed antiproliferative effects against tumor cells (HepG2, PC-3, and HT-29). The new compounds showed late apoptotic and necrotic effects in HepG2 cells. [ABSTRACT FROM AUTHOR]

Increasing abundance of arborescent octocorals (often referred to as gorgonians) on Caribbean reefs raises the question of whether habitat structure provided by octocorals can mediate a transition between coral- and algal- dominated states by increasing fish abundance and herbivory. This study tested the hypotheses that feeding rates and densities of demersal reef fishes are affected by the habitat structure provided by dense octocoral communities. Surveys of fishes on coral reefs in St John, US Virgin Islands, found 1.7-fold higher densities, and 2.4-fold higher feeding rates within versus outside of dense octocoral canopies. This difference, however, was only seen at sites with octocoral densities > 8 colonies m−2. Furthemore, the proximity of octocoral colonies to fish had an effect on the grazing rate of key herbivores (surgeonfishes and parrotfishes), with a 53% higher feeding rate (1.90 ± 0.11 bites min−1 m−2) near octocorals (< 20 or 30 cm, depending on the site) versus farther from them (1.24 ± 0.09 bites min−1 m−2). Finally, within the canopy of dense octocoral communities (17 colonies m−2), reef fishes fed at a rate that was 2.2-fold higher within the community than at the edge of the community that faced an adjacent sand patch. Fish abundance, however, was not uniformly higher within versus at the edge of the octocoral community, as ecotone specialists such as gobiids, blennioids, ostraciids, holocentrids, labrids, and pomacentrids were 1.3—2.3 times more abundant at the edge. In contrast, other taxa of demersal fishes, notably herbivores, were twice as abundant within octocoral communities than at the edges. Together, these results reveal an association between habitat structure created by octocorals on shallow reefs and increased feeding rates of demersal fishes (including those of herbivores). The potential of octocorals to increase herbivory that could mediate stony coral recovery is therefore worthy of further study. [ABSTRACT FROM AUTHOR]

Neoparmulella periperiensis gen. et sp. nov. is a new siphonostomatoid copepod found associated with an endemic octocoral Phyllogorgia dilatata (Esper) in Bahia State, northeastern region of Brazil. The new genus belongs to the subfamily Parmulodinae which is recorded for the first time in the South Atlantic Ocean. The new genus differs from all other entomolepidids once it shows the fourth leg biramous and 3-segmented. The third endopodal segment of leg 4 also bears reduced setation. Modifications on the family diagnosis are proposed in order to accommodate the new genus and recent species described. The validity of the subfamilies is also discussed. MS - Genus - species - [ABSTRACT FROM AUTHOR]

Corals are the ecosystem engineers of coral reefs, one of the most biodiverse marine ecosystems. The ability of corals to form reefs depends on the precipitation of calcium carbonate (CaCO3) under biological control. However, several mechanisms underlying coral biomineralization remain elusive, for example, whether corals employ different molecular machineries to deposit different CaCO3 polymorphs (i.e. aragonite or calcite). Here, we used tandem mass spectrometry (MS/MS) to compare the proteins occluded in the skeleton of three octocoral and one scleractinian species: Tubipora musica and Sinularia cf. cruciata (calcite sclerites), the blue coral Heliopora coerulea (aragonitic skeleton), and the scleractinian aragonitic Montipora digitata. Reciprocal Blast analysis revealed extremely low overlap between aragonitic and calcitic species, while a core set of proteins is shared between octocorals producing calcite sclerites. However, the carbonic anhydrase CruCA4 is present in the skeletons of both polymorphs. Phylogenetic analysis highlighted several possible instances of protein co-option in octocorals. These include acidic proteins and scleritin, which appear to have been secondarily recruited for calcification and likely derive from proteins playing different functions. Similarities between octocorals and scleractinians included presence of a galaxin-related protein, carbonic anhydrases, and one hephaestin-like protein. Although the first two appear to have been independently recruited, the third appear to share a common origin. This work represents the first attempt to identify and compare proteins associated with coral skeleton polymorph diversity, providing several new research targets and enabling both future functional and evolutionary studies aimed at elucidating the origin and evolution of coral biomineralization. [ABSTRACT FROM AUTHOR]

Octocorals are important zoobenthic organisms, contributing to structural heterogeneity and species diversity on hardgrounds. Their persistence amidst global coral reef degradation and ocean acidification, has prompted renewed interest in this taxon. Octocoral assemblages at 52 sites in continental Ecuador and Galápagos (23 species, 3742 colonies) were examined for composition, size distributions within and among populations, and connectivity patterns based on ocean current models. Species richness varied from 1 to 14 species per site, with the richest sites on the continent. Three assemblage clusters were recognised based on species richness and population size, one with a mix of sites from the mainland and Galápagos (defined by Muricea fruticosa and Leptogorgia alba, Muricea plantaginea and Pacifigorgia darwinii), the second from Santa Elena in southern Ecuador (defined by M. plantaginea and L. alba) and the third from the northernmost sites on the continent, in Esmeraldas (defined by Muricea fruticosa, Heterogorgia hickmani, Leptogorgia manabiensis). Based on biophysical larval flow models with 30, 60, 90-day Pelagic Larval Duration, good connectivity existed along the South American mainland, and from the continent to Galápagos. Connectivity between Galápagos, Cocos, Malpelo and the Colombian mainland may explain the wide distribution of L. alba. Muricea plantaginea had the densest populations with the largest colonies and therewith was an important habitat provider both in continental Ecuador and Galápagos. Continental Ecuador harbours the most speciose populations of octocorals so far recorded in the southern Eastern Tropical Pacific (ETP). Most species were uncommon and possibly vulnerable to local extirpation. The present study may serve as a base line to determine local and regional impacts of future disturbances on ETP octocorals. [ABSTRACT FROM AUTHOR]

Coral reefs throughout the tropics have experienced large declines in the abundance of scleractinian corals over the last few decades, and some reefs are becoming functionally dominated by animal taxa other than scleractinians. This phenomenon is striking on many shallow reefs in the tropical western Atlantic, where arborescent octocorals now are numerically and functionally dominant. Octocorals are one of several taxa that have been overlooked for decades in analyses of coral reef community dynamics, and our understanding of why octocorals are favoured (whereas scleractinians are not) on some modern reefs, and how they will affect the function of future reef communities, is not commensurate with the task of scientifically responding to the coral reef crisis. We summarize the biological and ecological features predisposing octocorals for success under contemporary conditions, and focus on those features that could have generated resistance and resilience of octocoral populations to environmental change on modern reefs. There is a rich set of opportunities for rapid advancement in understanding the factors driving the success of octocorals on modern reefs, but we underscore three lines of inquiry: (1) the functional implications of strongly mixotrophic, polytrophic, and plastic nutrition, (2) the capacity to recruit at high densities and maintain rapid initial rates of vertical growth, and (3) the emergent properties associated with dense animal forests at high colony densities. [ABSTRACT FROM AUTHOR]

The attractiveness of natural and cultured pearls is related, in part, to their colour. Raman spectroscopy was applied to natural and cultured pearls and the shells from 23 species of pearl producing molluscs, thus covering the majority of the natural and cultured pearls and their host animals found in the market today, in order to better understand their colouration and eventually to be able to identify the host animal. Pigments with a polyenic chain were identified in samples from 18 species. The vast majority were found to be a mixture of partially methylated polyenes, similar to those observed in octocorals. Samples with a similar colour but from different animal species can be sometimes related to a significantly different mixtures of such pigments. Only natural pearls from Pinna nobilis were found to be coloured by carotenoids. Raman spectra of other pigments were also observed on samples from four species, possibly linked with pigments from the porphyrin group. [ABSTRACT FROM AUTHOR]

The octocoral, Paramuricea clavata, is a habitat-forming anthozoan with a key ecological role in rocky benthic and biodiversity-rich communities in the Mediterranean and Eastern Atlantic. Shallow populations of P. clavata in the North-Western Mediterranean are severely affected by warming-induced mass mortality events (MMEs). These MMEs have differentially impacted individuals and populations of P. clavata (i.e., varied levels of tissue necrosis and mortality rates) over thousands of kilometers of coastal areas. The eco-evolutionary processes, including genetic factors, contributing to these differential responses remain to be characterized. Here, we sequenced a P. clavata individual with short and long read technologies, producing 169.98 Gb of Illumina paired-end and 3.55 Gb of Oxford Nanopore Technologies (ONT) reads. We obtained a de novo genome assembly accounting for 607 Mb in 64,145 scaffolds. The contig and scaffold N50s are 19.15 Kb and 23.92 Kb, respectively. Despite of the low contiguity of the assembly, its gene completeness is relatively high, including 75.8% complete and 9.4% fragmented genes out of the 978 metazoan genes contained in the metazoa_odb9 database. A total of 62,652 protein-coding genes have been annotated. This assembly is one of the few octocoral genomes currently available. This is undoubtedly a valuable resource for characterizing the genetic bases of the differential responses to thermal stress and for the identification of thermo-resistant individuals and populations. Overall, having the genome of P. clavata will facilitate studies of various aspects of its evolutionary ecology and elaboration of effective conservation plans such as active restoration to overcome the threats of global change. [ABSTRACT FROM AUTHOR]

Indo-Pacific lionfish have become invasive throughout the western Atlantic. Their predatory effects have been the focus of much research and are suggested to cause declines in native fish abundance and diversity across the invaded range. However, little is known about their non-consumptive effects, or their effects on invertebrates. Lionfish use shelters on the reef, thus there is potential for competition with other shelter-dwelling organisms. We demonstrate similar habitat associations between invasive lionfish, native spiny lobsters (Panulirus argus) and native long-spined sea urchins (Diadema antillarum), indicating the potential for competition. We then used a laboratory experiment to compare activity and shelter use of each species when alone and when lionfish were paired with each native species. Spiny lobsters increased their activity but did not change their shelter use in the presence of a lionfish, whilst long-spined sea urchins changed neither their activity nor shelter use. However, lionfish reduced their shelter use in the presence of spiny lobsters and long-spined sea urchins. This study highlights the importance not only of testing for the non-consumptive effects of invasive species, but also exploring whether native species exert non-consumptive effects on the invasive. [ABSTRACT FROM AUTHOR]

The species of the genus Psammogorgia Verrill, 1868 from the shallow waters of the tropical eastern Pacific were mainly described from 1846 to 1870. Very few contributions were published subsequently. Recently, the genus was revisited with the addition of two new species. However, a comprehensive generic study is still missing for the eastern Pacific. Psammogorgia is characterised by having axes cores without mineralisation, mainly coarse irregular spindles and thorny, leafy or tuberculate clubs coenenchymal sclerites and the anthocodial armature with distinct collaret and points arrangements. Herein a taxonomic revision of the genus is presented based on type material which was morphologically analysed and illustrated using optical and scanning electron microscopy. Comparative character tables are provided for comparison among species in the genus, along with a taxonomic key. Moreover, the taxonomic status of each species was analysed. The genus Psammogorgia comprises six valid species and two varieties, and three lectotypes and a new combination are proposed to establish the taxonomic status of these species. [ABSTRACT FROM AUTHOR]

A 15‐year old, female miniature dachshund was presented for a 5‐year history of progressive dyspnoea. A laryngeal mass was detected on cervical radiographs. Surgery was performed 5 days later to reduce the volume of the mass and to obtain biopsy samples; the dyspnoea improved. Based on the histopathological findings, the mass was diagnosed as inflammatory polyp. This case of inflammatory polyp in the larynx suggests that it should be listed in the differential diagnoses for laryngeal masses in dogs and that surgical treatment may be curative. [ABSTRACT FROM AUTHOR]

Due to difficulties with surveying, cryptobenthic fishes are understudied, which limits assessments of their extinction risk. Using a novel survey method (a combination of underwater visual census and underwater biofluorescence census), we surveyed the densities of two gorgonian seafan-associated pygmy seahorses (Hippocampus bargibanti and H. denise) in 63 sites across Indonesia. Densities were low for both seahorses (≤ 0.4 seahorses/host) and their hosts (≤ 0.25 seafans/200 m2). These low densities, combined with habitat specialisation, increase extinction risk. We failed to detect regional or depth patterns in seahorse abundance per host or group size in occupied hosts. Host occupancy rates were low (< 13.7%), suggesting that other factors than habitat availability constrain pygmy seahorse population size. The low densities found in this study highlight the high extinction vulnerability for habitat specialists on coral reefs. [ABSTRACT FROM AUTHOR]

An increasing number of studies have shown that anthropogenic noise can negatively affect aspects of the anti-predator behaviour of reef fishes, potentially affecting fitness and survival. However, it has been suggested that effects could differ among noise sources. The present study compared two common sources of anthropogenic noise and investigated its effects on behavioural traits critical for fish survival. In a tank-based experiment we examined the effects of noise from 4-stroke motorboats and ships (bulk carriers > 50,000 tonnes) on the routine swimming and escape response of a coral reef fish, the whitetail damselfish (Pomacentrus chrysurus). Both 4-stroke boat and ship noise playbacks affected the fast-start response and routine swimming of whitetail damselfish, however the magnitude of the effects differed. Fish exposed to ship noise moved shorter distances and responded more slowly (higher response latency) to the startle stimulus compared to individuals under the 4-stroke noise treatment. Our study suggests that 4-stroke and ship noise can affect activity and escape response of individuals to a simulated predation threat, potentially compromising their anti-predator behaviour. [ABSTRACT FROM AUTHOR]

In the present study, we assessed prokaryotic communities of demosponges, a calcareous sponge, octocorals, sediment and seawater in coral reef habitat of the central Red Sea, including endemic species and species new to science. Goals of the study were to compare the prokaryotic communities of demosponges with the calcareous sponge and octocorals and to assign preliminary high microbial abundance (HMA) or low microbial abundance (LMA) status to the sponge species based on compositional trait data. Based on the compositional data, we were able to assign preliminary LMA or HMA status to all sponge species. Certain species, however, had traits of both LMA and HMA species. For example, the sponge Ectyoplasia coccinea, which appeared to be a LMA species, had traits, including a relatively high abundance of Chloroflexi members, that were more typical of HMA species. This included dominant OTUs assigned to two different classes within the Chloroflexi. The calcareous sponge clustered together with seawater, the known LMA sponge Stylissa carteri and other presumable LMA species. The two dominant OTUs of this species were assigned to the Deltaproteobacteria and had no close relatives in the GenBank database. The octocoral species in the present study had prokaryotic communities that were distinct from sediment, seawater and all sponge species. These were characterised by OTUs assigned to the orders Rhodospirillales, Cellvibrionales, Spirochaetales and the genus Endozoicomonas, which were rare or absent in samples from other biotopes. [ABSTRACT FROM AUTHOR]

A rare case of uterine leiomyosarcoma associated with chondriod metaplasia, cystic endometrial polyps and uterine horn intussusception in a greater cane rat was macroscopically, histopathologically, immuno-histochemically and ultrastructurally evaluated. The histopathological findings for this tumour were similar to those for leiomyosarcomas described in other species. Immunohistochemical examination demonstrated positive immunoreactivity of neoplastic cells with a-smooth muscle actin, desmin and vimentin. Ultrastructurally, nuclear and cytoplasmic features were consistent with leiomyosarcoma. These results revealed the tumour to be of smooth muscle origin. To our knowledge, this is the first reported case of uterine leiomyosarcoma associated with cystic endometrial polyps, chondriod metaplasia and uterine horn intussusception in a greater cane rat. [ABSTRACT FROM AUTHOR]

Sea pens are ecologically important habitats for associated marine organisms, serving as ecosystem engineers in sandy or muddy seafloor environments. In such areas, sea pens can form habitats with high population densities known as "sea pen fields". However, the presence and importance of sea pen fields have not been well studied in shallow waters in East Asia. Here, we report a sea pen field of Virgularia sp. aff. gustaviana in the shallow waters of Ushibuka Marine Park, in the Amakusa Islands of southern Japan. The average colony numbers of the field across all depths (7-20 m) was 10.3 colonies/m2 (live colonies) to 13.6 colonies/m2 (all: live+dead colonies+holes), and the area of the sea pen field was at least 50,000 m2. At a depth of 15 m, the substratum consisted of sand and fallen leaves of terrestrial origin, and the highest sea pen density was observed (averages=17.2 live colonies/m2, =25.8 total (live+dead+holes) colonies/m2). At a depth of 20 m, the substratum consisted of broken shells and rocks and had the lowest density (live colonies: average=0.8 colonies/m2, all: average=1.0 colonies/m2). There were significant differences in colony number of Virgularia sp. aff. gustaviana between the "sand", "sand+leaves", and "broken shells/rocks" substrates. We hypothesize that the strength of the water currents caused by local geographic features and tidal movements produce suitable sedimentation and habitat for this species of sea pen. Therefore, we suggest that preserving the natural coastline is crucial to protect this and other sea pen fields in shallow waters and their benthic marine communities. [ABSTRACT FROM AUTHOR]