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ObjectiveIn the present study, we aimed to assess the cognition of post-COVID-19 condition (PCC) participants in relation to their subjective sleep quality (Pittsburgh Sleep Quality Index, PSQI) and to analyse possible moderators of this effect, such as quality of life (European Quality of Life-5 Dimensions, EQ-5D), fatigue (Chadler Fatigue Questionnaire, CFQ), cognitive reserve (Cognitive Reserve Questionnaire, CRC), and subjective cognitive complaints (Memory Failures of Everyday Questionnaire, MFE-30).MethodsWe included 373 individuals with PCC and 126 healthy controls (HCs) from the NAUTILUS Project (NCT05307549 and NCT05307575) who were assessed with a comprehensive neuropsychological battery and various questionnaires.ResultsWe found that PCC participants with poor sleep quality had a 4.3% greater risk of immediate verbal memory deficits than those with good sleep quality, as indicated by the greater odds ratio (OR) of 1.043 and confidence interval (CI) of 1.023–1.063. Additionally, their risk of immediate verbal memory disorders was multiplied by 2.4 when their EQ-5D score was low (OR 0.33; CI 0.145–0.748), and they had a lower risk of delayed visual memory deficits with a greater CRC (OR 0.963; CI 0.929–0.999). With respect to processing speed, PCC participants with poor sleep quality had a 6.7% greater risk of deficits as the MFE increased (OR 1.059; CI 1.024–1.096), and the risk of slowed processing speed tripled with a lower EQ-5D (OR 0.021; CI 0.003–0.141).ConclusionThese results indicate that poor subjective sleep quality is a potential trigger for cognitive deficits. Therapeutic strategies to maximize sleep quality could include reducing sleep disturbances and perhaps cognitive impairment in PCC individuals.

Marco contextual: Durante el siglo XIX, la transformación económica derivada de la revolución industrial provocó el pauperismo en las personas vulnerables, hecho que les deterioró su salud. Objetivo: Revisar historiográficamente el pensamiento de Concepción Arenal Ponte, desde una perspectiva enfermera. Metodología: Investigación social, histórica y cualitativa, relacionada con los cuidados de enfermería proporcionados de una forma holística a la persona vulnerable, a través del pensamiento social de Concepción Arenal. Recopilación de información: en fuentes primarias y secundarias. Resultados: Su obra La beneficencia, la filantropía y la caridad es una defensa cerrada del cuidado del paciente en su domicilio. Y para evitar la repulsa que, por entonces, causaban los hospitales y el personal de enfermería, Arenal defendió que dicho cuidado fuese realizado por enfermeras profesionales bien formadas y entrenadas. Conclusión: El pensamiento de Arenal, mujer muy destacada dentro del campo del derecho, comienza a ser actualmente muy relevante dentro del campo sanitario y cobra un especial interés tanto en el área social como en el área de la salud comunitaria.

Introduction: Pediatric extreme hydrocephalus (PEH) is a complex condition with uncertain prognostic outcomes. Objectives: In this study, we analyze the long-term change in cerebral parenchymal thickness of pediatric patients with extreme hydrocephalus after shunting. Methods: A retrospective observational study was conducted on patients with PEH treated at the General Hospital of Mexico from 2009 to 2016. Cerebral parenchymal thickness was measured in computed tomography studies before surgical intervention, at 5 months, and 4 years post-surgery. The average change in thickness in millimeters was analyzed, and its normalized value was assessed using the Wilcoxon test and the R2 of a linear function. Results: Twelve patients, including 5 females and 7 males, were studied. Nine cases were congenital, while the remaining 3 cases were 6 months, 7, and 10-years-old. At 5.2 months PostOp., patients showed an average increase of 160% in cerebral parenchymal thickness, which increased to 270% at 4.3 years. However, 4 cases (33.3%) had an increase of < 10% from the original thickness. One patient at 4.3 years exhibited a 594% increase compared to the baseline. In the congenital cases, pre-operative thickness had a good correlation (R2 = 0.70) with post-operative thickness, but pre-operative age of patients did not correlate with post-operative thickness (R2 = 0.03). Conclusions: In this study, 3 out of 12 cases of PEH showed a long-term increase of > 200% compared to preoperative thickness. Even in cases of extreme hydrocephalus, significant long-term improvement can occur. Rapid treatment of these cases is crucial to increase the likelihood of improvement.

The pandemic caused by covid-19 has revealed an enormous vulnerability in health, economic and social terms. We are facing a new unprecedented environmental and socioeconomic reality, which poses a challenge for risk control and management. This forces a methodological effort to be made in all areas in the creation of models for the prevention and prediction of existing impacts, risks, and potential threats. In Social Services’ field, we propose a tool that will allow, on the one hand, to measure the impact of COVID-19 on the Social Services system and, on the other hand, to evaluate the areas with the highest socioeconomic risk in the post-COVID scenario. The modified Delphi technique has been used to reach a consensus on a proposal of 37 indicators with a group of experts, which in the final phase resulted in 34. To our knowledge, this represents the first risk assessment tool in social services designed specifically for use at local level. The proposed tool can help determine the situation of municipal social services in relation to risk, assessing weaknesses and improving the capacity of the system.

La inteligencia artificial generativa (IAG) constituye una forma compleja y efectiva de tecnología de aprendizaje automático que suscita un amplio debate social acerca de sus límites y posibilidades. En este sentido, una mirada analítica desde la ecología de medios nos ofrece un marco interesante para identificar interrelaciones entre los distintos problemas y desafíos derivados de esta tecnología emergente. Este artículo delimita los rasgos de la IA Generativa como medio inscrito en la ecología mediática y recurre a las herramientas conceptuales de las leyes de los medios que formularon Marshall y Eric McLuhan para reflexionar sobre la obra creativa en la era de la automatización: las leyes de la extensión, obsolescencia, recuperación y reversión. Entre las cuestiones abordadas destacan las implicaciones de la IA Generativa como tecnología de codificación de las representaciones, la pérdida de la referencialidad y trazabilidad de la obra, el futuro de la propiedad intelectual y su impacto en las industrias creativas, así como el retorno y revalorización de las experiencias compartidas o la performance.

Introducción: La formación del profesorado para las competencias inclusivas ha ganado importancia recientemente ya que los profesores reconocen la necesidad de estar plenamente formados para ofrecer la mejor respuesta educativa al alumnado con necesidades educativas especiales. El objetivo de esta investigación es realizar una Revisión Sistemática y analizar las publicaciones en base a sus características, temas de investigación y competencias docentes, siguiendo las directrices PRISMA. Método: Se realiza una búsqueda en las bases de datos de Scopus y WoS obteniendo 75 publicaciones entre 2016 y 2022. Se plantean 10 preguntas de investigación enmarcadas dentro de 3 áreas temáticas. Resultados: Los resultados dan respuesta a las preguntas planteadas destacando las características de los artículos, la metodología y método que se ha utilizado en cada estudio, así como las competencias estudiadas. Discusión: Se concluye que las competencias con mayor análisis de estudio están parcialmente relacionadas con las propuestas por la Agencia Nacional de Evaluación de Calidad y Acreditación (ANECA) en los Libros Blancos. A la luz de los resultados, se proponen programas formativos para los docentes que contemplen contenido vinculado con competencias específicas para favorecer la inclusión educativa del alumnado con necesidades educativas especiales.

Molecular clock estimates suggest the origin of Annelida dates back to the Ediacaran period, which is in discordance with the first appearance of this taxon in the early Cambrian, as evidenced by the fossil records of stem-group and basally branching crown-group annelids. Using new material from the early Cambrian Guanshan biota (Cambrian Series 2, Stage 4), we re-interpret Gaoloufangchaeta bifurcus Zhao, Li & Selden, 2023, as the earliest known errantian annelid. Gaoloufangchaeta has a prominent anterior end bearing three pairs of putatively sensory appendages and a pair of anterior eyes; a muscular eversible pharynx with papillae is identified. The presence of enlarged parapodia with acicula-like structures and long capillary chaetae suggests a pelagic lifestyle for this taxon. Our phylogenetic analyses recover Gaoloufangchaeta within the Phyllodocida (Pleistoannelida, Errantia), extending the origin of Errantia back to the early Cambrian. Our data are in line with the hypothesis that Annelida diverged before the Cambrian and indicate both morphological and ecological diversification of annelids in the early Cambrian.

Objetivo. Ante la escasez de investigaciones que traten de manera conjunta el conocimiento, la calidad y la higiene del sueño en el adolescente, el objetivo del presente estudio es analizar las diferencias y las relaciones que existen entre estas variables en función de características sociodemográficas y personales. Método. A través de los instrumentos Sleep Beliefs Scale (SBS), Spanish Adolescents and Young Adults Pittsburgh Sleep Quality Index (AYA-PSQI-S) y Adolescents Sleep Hygiene Scale Revied (ASHSr), se midieron las características del sueño de 140 estudiantes (M = 16,75; DE = 0,75). Resultados. El 89,3% de los adolescentes presentaban problemas de sueño, subyacentes de la mejorable higiene del sueño por parte del 62,2% y de un escaso conocimiento general del sueño. El sexo femenino y el alumnado en cursos superiores presentaron mayores problemas para conciliar el sueño, somnolencia y una baja gestión cognitiva-emocional (p < 0,05). La higiene del sueño (β = - 0,344), la edad (β = 0,154) y el autoconcepto (β = -0,349) son los factores que predijeron significativamente (p < 0,05) la calidad del sueño del adolescente. Conclusiones. La adquisición de una adecuada higiene del sueño se vuelve fundamental para mejorar la calidad del descanso y la funcionalidad diurna en adolescentes, destacando su importancia, sobre todo, en niveles académicos avanzados y en el caso específico de las mujeres.

The utilization of a novel (systemic) biofertilizer containing Pseudomonas fluorescens, Azospirillum brasilense, and Bacillus subtilis and possessing the technology to facilitate the entry of bacteria through the stomata, was evaluated at three localities in Mexico (Potrero Nuevo, Veracruz; Ameca, Jalisco; and Champotón, Campeche) in two sugarcane varieties (NCO-310 and Mex 57–473) at different time scales. Inoculation of the systemic biofertilizer was imposed over the local agricultural management of the sugarcane; chemical fertilization of the experimental parcels at Potrero Nuevo was done using 70-20-20 and 120-80-80 at Ameca and Champotón. Three doses of the biofertilizer per hectare were applied during the annual productive cycle of sugarcane at each site; one year at Potrero Nuevo and Champotón; and six years at Ameca. The annual sugarcane yield was evaluated at each site. Additionally, sugar quality (°Brix or sucrose content) was evaluated at the three localities, while different variables of stalk performance were also measured at Ameca and Champotón. Our data provide evidence that this systemic biofertilizer consistently and reliably increased the sugarcane yield at all localities during the time of evaluation, ranging from 73.7 tons ha−1 at Potrero Nuevo (2.5 times increase; P

Although widely used for energy production, fossil fuels pose a challenge in the fight against climate change. They are used in various sectors, with construction accounting for 40% of total energy demand.. To address this issue, the article looks at alternative technologies and fuels that can effectively reduce our dependence on fossil fuel-based energy, reduce our carbon footprint, and make existing buildings more self-sufficient. Hydrogen-powered fuel cells have the potential to completely transform energy production in buildings, generating energy on-site and reducing the carbon footprint of existing constructions. This article compares four commercial fuel cell options (SOFC and PEMFC) based on their technical, regulatory, and economic viability. Moreover, the selected equipments, suitable for domestic use and scalable, will be evaluated for their integration into an existing building to provide a proportion and knowledge of space. In conclusion, Equipment B (PEMFC) was chosen for installation after carefully considering the spatial and technical requirements that had to be met. The low maintenance costs of Equipment B played a crucial role, and the use of disruptive technology was in line with the case study strategy. Some parameters, such as space, ventilation, and temperature, are future fuel cell policy benchmarks. The comparison of PEMFC shows a developing competitive sector applicable to our building.

Background This study performs an exploratory analysis of current-future sustainability challenges for ocean planning for the regional seas of Catalonia located in the Western Mediterranean (Spain). Methods To address the challenges we develop an Maritime Spatial Planning (MSP)-oriented geodatabase of maritime activities and deploy three spatial models: 1) an analysis of regional contribution to the 30% protection commitment with Biodiversity Strategy 2030; 2) a spatial Maritime Use Conflict (MUC) analysis to address current and future maritime activities interactions and 3) the StressorGenerator QGIS application to locate current and anticipate future sea areas of highest anthropogenic stress. Results & Conclusions Results show that the i) study area is one of the most protected sea areas in the Mediterranean (44–51% of sea space protected); ii) anthropogenic stressors are highest in 1–4 nautical miles coastal areas, where maritime activities agglomerate, in the Gulf of Roses and Gulf of Saint Jordi. iii) According to the available datasets commercial fishery is causing highest conflict score inside protected areas. Potential new aquaculture sites are causing highest conflict in Internal Waters and the high potential areas for energy cause comparably low to negligible spatial conflicts with other uses. We discuss the added value of performing regional MSP exercises and define five challenges for regional ocean sustainability, namely: Marine protection beyond percentage, offshore wind energy: a new space demand, crowded coastal areas, multi-level governance of the regional sea and MSP knowledge gaps.

IntroductionInertial measurement units (IMUs) are utilized to measure trunk acceleration variables related to both running performances and rehabilitation purposes. This study examined both the reliability and sex-based differences of these variables during an incremental treadmill running test.MethodsEighteen endurance runners performed a test–retest on different days, and 30 runners (15 females) were recruited to analyze sex-based differences. Mediolateral (ML) and vertical (VT) trunk displacement and root mean square (RMS) accelerations were analyzed at 9, 15, and 21 km·h−1.ResultsNo significant differences were found between test-retests [effect size (ES)

The paper analyses the degradation process of commercial ultramarine blue pigments in cementitious materials. For this purpose, two commercial pigments (with and without a protective coating) in different solutions and cement pastes are studied incrementally. The results show that pigment degradation occurs due to an ion exchange phenomenon; during hydration high ion contents are released, calcium and potassium being the most aggressive for the pigment. Calcium distorts the unit cell; between the sodium of the pigment and the potassium in the medium a cation exchange phenomenon takes place. Both processes lead to the diffusion of sulphate and sulphide ions from the pigment to the medium causing loss of colour and the formation of ettringite.

Los profesores José Sanmartín y José Alfredo Peris-Cancio han dedicado diferentes trabajos a lo largo de los últimos años a la elaboración de una propuesta metodológica de aplicación del personalismo integral de Juan Manuel Burgos y Karol Wojtyla a la reflexión filosófica y antropológica desde la experiencia cinemática. Su contribución se ha circunscrito principalmente al ámbito del Hollywood clásico y, en concreto, a la obra de directores como La Cava, Capra, McCarey, Leisen, Koster o Ford. El presente estudio parte de dicha propuesta, así como de trabajos específicamente dedicados a Ford, y tiene como objetivo descender al análisis y la lectura filosófica-fílmica de algunas de las películas “mudas” del genio de Maine, para esclarecer la eventual comparecencia de rasgos personalistas en los inicios de su obra.

IntroductionBreast cancer (BC) is the leading cause of cancer-related deaths among women, with triple-negative breast cancer (TNBC) representing one of the most aggressive and treatment-resistant subtypes. In this study, we aimed to evaluate the antitumor potential of C14 and P8 molecules in both TNBC and radioresistant TNBC cells. These compounds were chosen for their ability to stabilize the complex formed by the overactivated form of K-Ras4BG13D and its membrane transporter (PDE6δ).MethodsThe antitumor potential of C14 and P8 was assessed using TNBC cell lines, MDA-MB-231, and the radioresistant derivative MDA-MB-231RR, both carrying the K-Ras4B> G13D mutation. We investigated the compounds' effects on K-Ras signaling pathways, cell viability, and tumor growth in vivo.ResultsWestern blotting analysis determined the negative impact of C14 and P8 on the activation of mutant K-Ras signaling pathways in MDA-MB-231 and MDA-MB-231RR cells. Proliferation assays demonstrated their efficacy as cytotoxic agents against K-RasG13D mutant cancer cells and in inducing apoptosis. Clonogenic assays proven their ability to inhibit TNBC and radioresistant TNBC cell clonogenicity. In In vivo studies, C14 and P8 inhibited tumor growth and reduced proliferation, angiogenesis, and cell cycle progression markers.DiscussionThese findings suggest that C14 and P8 could serve as promising adjuvant treatments for TNBC, particularly for non-responders to standard therapies. By targeting overactivated K-Ras and its membrane transporter, these compounds offer potential therapeutic benefits against TNBC, including its radioresistant form. Further research and clinical trials are warranted to validate their efficacy and safety as novel TNBC treatments.

Adoptive cell therapy (ACT) comprises different strategies to enhance the activity of T lymphocytes and other effector cells that orchestrate the antitumor immune response, including chimeric antigen receptor (CAR) T-cell therapy, T-cell receptor (TCR) gene-modified T cells, and therapy with tumor-infiltrating lymphocytes (TILs). The outstanding results of CAR-T cells in some hematologic malignancies have launched the investigation of ACT in patients with refractory solid malignancies. However, certain characteristics of solid tumors, such as their antigenic heterogeneity and immunosuppressive microenvironment, hamper the efficacy of antigen-targeted treatments. Other ACT modalities, such as TIL therapy, have emerged as promising new strategies. TIL therapy has shown safety and promising activity in certain immunogenic cancers, mainly advanced melanoma, with an exciting rationale for its combination with immune checkpoint inhibitors. However, the implementation of TIL therapy in clinical practice is hindered by several biological, logistic, and economic challenges. In this review, we aim to summarize the current knowledge, available clinical results, and potential areas of future research regarding the use of T cell therapy in patients with solid tumors

Introduction Management controversy and clinical equipoise exist in treatments of long bone fractures and traumatic hip dislocation in paediatric patients due to the lack of high-quality clinical evidence. This protocol describes the effort of a large prospective global multicentre cohort study (registry) aiming at providing quality data to assist evidence-based treatment decision-making.Methods and analysis Eligible paediatric patients (N=750–1000) with open physes suffering from proximal humerus fractures, distal humerus fractures, proximal radius fractures, forearm shaft fractures, traumatic hip dislocations, femoral neck fractures or tibial shaft fractures will be recruited over a period of 24–36 months. Hospitalisation and treatment details (including materials and implants) will be captured in a cloud-based, searchable database. Outcome measures include radiographic assessments, clinical outcomes (such as range of motion, limb length discrepancies and implant removal), patient-reported outcomes (Patient Reported Outcomes Of Fracture, Patient-Reported Outcomes Measurement Information System (PROMIS) and EuroQol-5D (EQ-5D-Y)) and adverse events.Aside from descriptive statistics on patient demographics, baseline characteristics, types of fractures and adverse event rates, research questions will be formulated based on data availability and quality. A statistical analysis plan will be prepared before the statistical analysis.Ethics and dissemination Ethics approval will be obtained before patients are enrolled at each participating site. Patient enrolment will follow an informed consent process approved by the responsible ethics committee. Peer-reviewed publication is planned to disseminate the study results.Trial registration number NCT04207892.

Abstract Alternative lengthening of telomeres (ALT) is a telomere maintenance mechanism activated in ~10–15% of cancers, characterized by telomeric damage. Telomeric damage-induced long non-coding RNAs (dilncRNAs) are transcribed at dysfunctional telomeres and contribute to telomeric DNA damage response (DDR) activation and repair. Here we observed that telomeric dilncRNAs are preferentially elevated in ALT cells. Inhibition of C-rich (teloC) dilncRNAs with antisense oligonucleotides leads to DNA replication stress responses, increased genomic instability, and apoptosis induction selectively in ALT cells. Cell death is dependent on DNA replication and is increased by DNA replication stress. Mechanistically, teloC dilncRNA inhibition reduces RAD51 and 53BP1 recruitment to telomeres, boosts the engagement of BIR machinery, and increases C-circles and telomeric sister chromatid exchanges, without increasing telomeric non-S phase synthesis. These results indicate that teloC dilncRNA is necessary for a coordinated recruitment of DDR factors to ALT telomeres and it is essential for ALT cancer cells survival.

Abstract Endothelial dysfunction is a leading cause of corneal blindness in developed countries and the only available treatment is the endothelial transplantation. However, the limited availability of suitable donors remains a significant challenge, driving the exploration of alternative regenerative therapies. Advanced Therapy Medicinal Products show promise but must adhere to strict regulations that prohibit the use of animal-derived substances. This study investigates a novel culture methodology using Plasma Rich in Growth Factors (PRGF) as the only source of growth factors for primary cultures of human corneal endothelial cells (CECs). CECs were obtained from discarded corneas or endothelial rings and cultured in two different media: one supplemented with xenogeneic factors and other xenogeneic-free, using PRGF. Comprehensive characterization through immunofluorescence, morphological analyses, trans-endothelial electrical resistance measurements, RNA-seq, and qPCR was conducted on the two groups. Results demonstrate that CECs cultured in the xenogeneic-free medium exhibit comparable gene expression, morphology, and functionality to those cultured in the xenogeneic medium. Notably, PRGF-expanded CECs share 46.9% of the gene expression profile with native endothelium and express all studied endothelial markers. In conclusion, PRGF provides an effective source of xenogeneic-free growth factors for the culture of CECs from discarded corneal tissue. Further studies will be necessary to demonstrate the applicability of these cultures to cell therapies that make clinical translation possible.

Abstract Benthic annelids belonging to the family Syllidae (Annelida, Errantia, Phyllodocida) exhibit a unique reproduction mode called “schizogamy” or “stolonization”, in which the posterior body part filled with gametes detaches from the original body, as a reproductive unit (stolon) that autonomously swims and spawns. In this study, morphological and histological observations on the developmental processes during stolonization were carried out in Megasyllis nipponica. Results suggest that the stolon formation started with maturation of gonads, followed by the formation of a head ganglion in the anteriormost segment of the developing stolon. Then, the detailed stolon-specific structures such as stolon eyes and notochaetae were formed. Furthermore, expression profiles of genes involved in the anterior–posterior identity (Hox genes), head determination, germ-line, and hormone regulation were compared between anterior and posterior body parts during the stolonization process. The results reveal that, in the posterior body part, genes for gonadal development were up-regulated, followed by hormone-related genes and head-determination genes. Unexpectedly, Hox genes known to identify body parts along the anterior–posterior axis showed no significant temporal expression changes. These findings suggest that during stolonization, gonad development induces the head formation of a stolon, without up-regulation of anterior Hox genes.

Introducción. La implicación de los médicos asistenciales en tareas de investigación clínica presenta grandes diferencias entre las diversas instituciones Material y método. Estudio transversal basado en una encuesta enviada a los miembros del Grupo de Trabajo de Enfermedades Infecciosas (GTei) de la Sociedad Española de Medicina Interna (SEMI) durante el mes de mayo de 2022 Resultados. De 1.789 miembros del GTei, 169 miembros (9,45%) cumplimentaron la encuesta. La percepción de la conveniencia de participación de cada facultativo en una o varias líneas de investigación fue de 8 puntos (P25:7; P75: 9 puntos). La percepción sobre el estímulo para investigar del sistema sanitario o de la dirección del hospital fue de 2 puntos (1-4), respectivamente. El apoyo a la investigación fue valorado con de 5 (2-7) y 6 (3-7) puntos en relación con el jefe de servicio y los compañeros del departamento, respectivamente. Otros factores evaluados fueron la falta de tiempo por no poder reducir la actividad asistencial (9; 7-10 puntos), la priorización de las actividades de ocio durante el tiempo libre disponible (7; 5-8 puntos), la organización de la carga asistencial (6; 3-9 puntos), las dificultades en la coordinación con otros servicios clínicos o centrales (6; 5-7 puntos y 6; 5-8 puntos, respectivamente) Conclusiones. La investigación clínica es muy bien valorada por los internistas dedicados a la patología infecciosa. Las principales necesidades percibidas son un mayor apoyoinstitucional y de la dirección del hospital, una mejor organización del departamento, lacoordinación interdepartamental y disponer de más tiempo para esta actividad. Background. The involvement of attending physicians in clinical research activities differs greatly among institutions. Method. Cross-sectional study based on a survey submitted to the members of the Working Group on Infectious Diseases (GTei) of the Spanish Society of Internal Medicine (SEMI) during the month of May 2022. Results. Out of 1,789 members of the GTei, 169 members (9.45%) completed the survey. The perception of the convenience of participation of each physician in one or more lines of research was 8 points (P25:7; P75: 9 points). The perception of encouragement to do research by the health system or hospital management was 2 points (1-4), respectively. Support for research was rated at 5 (2-7) and 6 (3-7) points inrelation to the head of service and colleagues in the department, respectively. Other factors evaluated were the lack of time due to not being able to reduce the care activity (9; 7-10 points), prioritization of leisure activities during available free time (7; 5-8 points), organization of the care load (6; 3-9 points), difficulties in coordinating with other clinical or central services (6; 5-7 points and 6; 5-8 points, respectively). Conclusions. Clinical research is highly valued by internists dedicated to infectious diseases. The main perceived needs are greater institutional and hospital managementsupport, better organization of the department, interdepartmental coordination and more time for this activity.

Despite only being its 3rd edition, the QUANTUM MATTER international conference & expo (QUANTUMatter) has already become a reference event for communities involved in the study of quantum materials, quantum information and quantum technologies at large. This article reports on this meeting, which took place in Madrid, in June 2023, with 420 participants and keynote speakers from all areas of quantum technologies and quantum materials.

El presente trabajo parte de un estudio de análisis histórico y de opinión política que permitió comprender el cambio en las relaciones internacionales entre Irán y Estados Unidos, esto se logró aplicando el rigor metodológico de un estudio analítico, a partir de la teoría del realismo de un contexto históricamente antagónico. Siempre que pensamos en Medio Oriente, lo primero que nos viene a la mente son imágenes de guerra, terrorismo y conflictos entre países y grupos étnicos. Esto se debe a que la situación de conflicto en la región no se ha detenido, debido al enfrentamiento de dos naciones. La primera, y seguramente la más conocida, es Israel, que desde su fundación lucha por sobrevivir contra grupos terroristas y otros países; y la segunda es Irán, que, al menos desde 1979, encabeza la lista de ser el más grande y acérrimo enemigo no solo de Israel, sino de Estados Unidos y de la mayoría de las potencias occidentales. La República Islámica de Irán, Rusia y China son los países antagónicos por excelencia de Occidente. Irán es el país que más apoyo brinda a los enemigos de Israel y el que más duramente combate la injerencia occidental en Medio Oriente, pero siempre se deja de lado los motivos por los que Irán pasó de ser uno de los mayores aliados de Occidente a ser el mayor villano y rival. Estos motivos pueden ser religiosos, políticos, territoriales o incluso económicos.

Arcane (Netflix, 2021-) se ha convertido en un referente del género distópico televisivo. Su animación cuidada, la profundidad y diversidad de sus personajes, la complejidad de las tramas narrativas, la crítica sistémica y la apuesta transmedia convierten esta serie en un ejemplo de calidad. Por ello, este artículo busca examinar cómo se representan las relaciones de poder en un mundo estructurado en torno al binomio Piltóver/Zaun y cómo lo habitan unos personajes construidos desde distintos marcadores sociales —clase social, género, origen, racialización, etc.—. Un análisis que no solo nos permite identificar críticas sistémicas propias de la distopía, sino reflexionar sobre las posibles respuestas a las injusticias e imaginar salidas alternativas desde unos márgenes diversos.

ALK, ROS1, and RET fusions and MET∆ex14 variant associate with response to targeted therapies in non‐small‐cell lung cancer (NSCLC). Technologies for fusion testing in tissue must be adapted to liquid biopsies, which are often the only material available. In this study, circulating‐free RNA (cfRNA) and extracellular vesicle RNA (EV‐RNA) were purified from liquid biopsies. Fusion and MET∆ex14 transcripts were analyzed by nCounter (Nanostring) and digital PCR (dPCR) using the QuantStudio® System (Applied Biosystems). We found that nCounter detected ALK, ROS1, RET, or MET∆ex14 aberrant transcripts in 28/40 cfRNA samples from positive patients and 0/16 of control individuals (70% sensitivity). Regarding dPCR, aberrant transcripts were detected in the cfRNA of 25/40 positive patients. Concordance between the two techniques was 58%. Inferior results were obtained when analyzing EV‐RNA, where nCounter often failed due to a low amount of input RNA. Finally, results of dPCR testing in serial liquid biopsies of five patients correlated with response to targeted therapy. We conclude that nCounter can be used for multiplex detection of fusion and MET∆ex14 transcripts in liquid biopsies, showing a performance comparable with next‐generation sequencing platforms. dPCR could be employed for disease follow‐up in patients with a known alteration. cfRNA should be preferred over EV‐RNA for these analyses.