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Background The value of double filtration plasmapheresis (DFPP) in severe hypertriglyceridemia-induced pancreatitis (sHTGP) is controversial. This study aimed to investigate the efficacy of DFPP on clinical results in patients with sHTGP and the costs associated with the procedure. Methods Patients who underwent DFPP after admission between January 2016 and December 2021 were recruited. Data on lipid profile, clinical results, and costs were retrospectively collected and analyzed. Results Fifty sHTGP patients who received DFPP were enrolled. All of the lipid profile were significantly reduced and maintained a downward trend. The APACHE II score on admission was higher and the reduction after DFPP was more obvious (P P

We report an efficient strategy that combines organic photoredox and hydrogen atom transfer to deliver

BACKGROUND: Conventional radiation may either promote or inhibit tumor metastatic potential paradoxically, but the underlying mechanism remains vague, and the knowledge concerning the changes of metastatic potential after heavy ion radiation is still limited. METHODSs: we detected the changes of the metastasis capacities of two colorectal cancer (CRC) cell lines after γ- or c-ion radiation, and investigated the underlying molecular mechanisms using western blot, immunoprecipitation, LC-MS/MS and siRNA transfection. RESULTS: The change of the migration and invasion potential showed a completely reversed pattern in these two cell lines after γ-or carbon ion radiation both in vivo and vitro. Radiation induced EMT in DLD-1 cells, but MET in HCT116 cells, which was due to the opposite modulation of ANXA2/AKT/GSK3β signaling pathway in CRC cells. ANAX2 could bind directly with GSK3β and act as a negative regulator of its activation. Knocking-down ANXA2 gene not only decreased the migration in nonirradiated CRC cells, but also reversed the enhanced migration in irradiated DLD-1 cells and strengthened radiation-impaired migration in HCT116 cells. CONCLUSION: The change of cellular motility after radiation is independent of radiation type, but is correlated with the inherent of cells, and ANXA2 is applicable to be a potential target for radiotherapy.

Different organs undergo distinct transcriptional, epigenetic and physiological alterations that guarantee their functional maturation after birth. However, the roles of epitranscriptomic machineries in these processes remain elusive. Here, we show that RNA methyltransferase enzymes Mettl3 and Mettl14 highly express in murine hepatocytes at embryonic and neonatal stages, and gradually decline during the postnatal development. Liver-specific Mettl3 deficiency causes hepatocyte hypertrophy, liver injury and growth retardation. Transcriptomic and N6-methyl-adenosine (m6A) profiling identify the neutral sphingomyelinase, Smpd3, as a target of Mettl3. Decreased decay of Smpd3 transcripts due to Mettl3 deficiency results in sphingolipid metabolism rewiring, characterized by toxic ceramide accumulation and excessive sphingomyelin hydrolysis, leading to mitochondrial damage and elevated ER stress. Pharmacological inhibition of Smpd3 ameliorates the abnormality of Mettl3-deficent liver. Our findings demonstrate that Mettl3/m6A fine-tunes sphingolipid metabolism, highlighting the pivotal role of an epitranscriptomic machinery in coordinating metabolic homeostasis and functional maturation during postnatal liver development.

Maresin1 is a potent lipid mediator exhibiting potential anti-inflammatory activity in a variety of inflammatory diseases, however, the underlying mechanisms remain poorly understood. Excessive activation of NLRP3 inflammasome has been established in multiple inflammatory diseases. Here, we show that Maresin1 dose-dependently inhibited the NLRP3 inflammasome activation and subsequent caspase-1 activation and IL-1β secretion. This inhibitory effect could be reversed by KH7 and H89, the inhibitors of the cAMP-PKA signaling pathway. Activation of PKA kinase induced by Maresin1 led to the K63-linked ubiquitination of NLRP3 in macrophages. Maresin1 attenuated serum IL-1β secretion through inhibition of NLRP3 inflammasome in vivo using Nlrp3-deficient mouse models of lipopolysaccharide (LPS)-induced sepsis. Maresin1 also repressed MSU-induced peritonitis. This study suggests that Maresin1 is an inhibitor of NLRP3 inflammasome activation and can be used clinically in the treatment of NLRP3 inflammasome-driven inflammatory diseases.

One new spirocyclic lactone, terreinlactone C (1), and one new benzopyran derivative, 2,2-dimethyl-3-hydroxychroman-6-aldehyde (2), were discovered from the fungus Aspergillus terreus. The chemical structures of compounds 1 and 2 were elucidated by detailedly analyzing NMR and HRESIMS data. Compound 1 is the first natural product with a 1-oxaspiro[4.5]decan-2-one ring system and a possible biogenetic pathway is proposed. Two compounds were tested for their cytotoxic activities against five human cancer cell lines.[Formula: see text].

A highly regioselective approach to access amide enol carbamates and carbonates 5a-5c', 7a-7h, and 9 was developed through Cu(OTf)2-catalyzed reactions of ynamides 4 with t-butyl carbamates 2 and 8 and t-butyl carbonates 6. Moreover, this strategy was successfully applied to generate amide enol carbamates 11a-11s and 14a-14f from imides 10 and 13 with ynamides through an N-Boc cleavage-addition ring-opening process. A range of substituents was amenable to this transformation, and the desired amide enol carbamates and carbonates were obtained in moderate to good yields.

In this work, direct numerical simulation (DNS) is used to investigate how airfoil shape affects wake structure and performance during a pitching-heaving motion. First, a class-shape transformation (CST) method is used to generate airfoil shapes. CST coefficients are then varied in a parametric study to create geometries that are simulated in a pitching and heaving motion via an immersed boundary method-based numerical solver. The results show that most coefficients have little effect on the propulsive efficiency, but the second coefficient does have a very large effect. Looking at the CST basis functions shows that the effect of this coefficient is concentrated near the 25% mark of the foils chord length. By observing the thrust force and hydrodynamic power through a period of motion it is shown that the effect of the foil shape change is realized near the middle of each flapping motion. Through further inspection of the wake structures, we conclude that this is due to the leading-edge vortex attaching better to the foil shapes with a larger thickness around 25% of the chord length. This is verified by the pressure contours, which show a lower pressure along the leading edge of the better performing foils. The more favorable pressure gradient generated allows for higher efficiency motion.

Background: Total percutaneous closure of a femoral artery access site using Perclose ProGlide has become prevalent after percutaneous endovascular aortic repair and veno-arterial extracorporeal membrane oxygenation. Objective: To evaluate the safety and efficacy of total percutaneous closure of the femoral artery access site after veno-arterial extracorporeal membrane oxygenation compared with that after endovascular aortic repair. Methods: This was a retrospective observational study conducted over an almost 4-year period and included 88patients who underwent endovascular aortic repair (64 patients) and veno-arterial extracorporeal membrane oxygenation (24 patients). The femoral artery puncture sites of the patients were closed percutaneously using Perclose ProGlide devices. Technical success was defined as successful arterial closure of the common femoral artery, without additional surgical or endovascular procedures. Access site complications, such as major bleeding requiring transfusion or surgical intervention, minor bleeding, groin infection, pseudoaneurysm, and lymphocele, were recorded 24 h and 30 days after arterial closure. Results: Each group's technical success rates were 95.8% (veno-arterial extracorporeal membrane oxygenation) and 92.2% endovascular aortic repair, respectively. There were no differences in the periprocedural complications of major bleeding, pseudoaneurysm, minor bleeding, acute limb ischemia, and groin infection. Furthermore, no arterial thrombosis, dissection, stenosis, arteriovenous fistula, hematoma, groin infection, or lymphocele was observed at the patients' access site who underwent follow-up ultrasound examination. There was no significant difference in the technical success rate of percutaneous closure by the Perclose ProGlide device in the endovascular aortic repair and veno-arterial extracorporeal membrane oxygenation groups. Also, no periprocedural or 30-day complications were observed at the access site of the endovascular aortic repair and veno-arterial extracorporeal membrane oxygenation patients.