201. 间断及持续缺氧扩张条件下大鼠真皮层组织重构特点的研究.
- Author
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崔江波, 张钰, 余州, and 马显杰
- Subjects
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PROLIFERATING cell nuclear antigen , *TISSUE expansion , *VISUAL fields , *PLASTIC surgery , *ANIMAL disease models - Abstract
Objective To explore the dermal histological changes of rat expanded skin under persistent tissue hypoxic expansion and intermittent tissue hypoxic expansion. Methods From November 2016 to June 2017, a rat model was used in the Plastic Surgery Department of Xijing Hospital of the Air Force Medical University. After intermittent and continuous hypoxic tissue expansion, the skin samples of rats were stained with HE and Masson staining to observe the changes in the structure and collagen content of the dermis; Proliferating cell nuclear antigen (PCNA) and Terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining were used to determine the proliferation and apoptosis of dermal fibroblasts; CD31 staining was used to analyze the changes of vascular morphology and capillary density in the dermis. Results The dermal thicknesses in the IH group, PH group, and the normal group were(500.20±22.57)μm. (464.30±27.23)μm, and(759.40±27.23)μm respectively. The dermal thickness of the PH group was significantly smaller than the IH group (P<0.05). The collagen content in the IH group and PH group was significantly higher than that in the normal skin group (P<0.05). The densities of PCNA-positive fibroblasts in the dermis of the normal skin group, IH group, and PH group were 6.10 per high power field, 19.38 per high power field, and 15.82 per high power field respectively. The number of positive cells in the IH group was higher than that in the PH group (P<0.05). The number of TUNEL-positive fibroblasts in the dermis of the normal skin group, IH group, and PH group was 5.10 per high-power field, 16.05 per high-power field, and 13.05 per high-power field. The density of TUNEL-positive fibroblasts in the IH group was higher than that in the PH group, but no statistically significant difference was found between the two groups (P>0.05). The average numbers of capillaries per high-power visual field in the dermis of the IH group and PH group were 22.87±1.13 and 13.57± 0.43, a statistically significant difference was found between the two groups (P<0.05). Conclusion Intermittent hypoxic tissue expansion can more effectively stimulate the proliferation of dermal fibroblasts, reduce cell apoptosis, promote collagen synthesis, and reconstruct the dermal vascular network, which is more conducive to the biological growth of the expanded skin. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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