201. Peroxiredoxin 2 is required for the redox mediated adaptation to exercise
- Author
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Qin Xia, Jose C. Casas-Martinez, Eduardo Zarzuela, Javier Muñoz, Antonio Miranda-Vizuete, Katarzyna Goljanek-Whysall, and Brian McDonagh
- Subjects
Organic Chemistry ,Clinical Biochemistry ,Biochemistry - Abstract
Exercise generates a site-specific increase in Reactive Oxygen Species (ROS) within muscle required for a beneficial adaptive response by activation of specific signalling pathways. Here, we demonstrate that Peroxiredoxin 2 (Prdx2), an abundant cytoplasmic 2-Cys peroxiredoxin, is required for the adaptive beneficial hormesis response to H2O2. A short bolus addition of H2O2increases mitochondrial capacity and improves myogenesis of cultured myoblasts, this beneficial adaptive response was suppressed in myoblasts with decreased expression of cytoplasmic Prdxs. A swimming exercise protocol inC. elegansincreased mitochondrial content, fitness, survival and longevity in wild type (N2) worms. In contrast,prdx-2mutant worms had decreased fitness, disrupted mitochondria, reduced survival and lifespan following exercise. Global proteomics following exercise identified distinct changes in the proteome of N2 andprdx-2mutants. Furthermore, a redox proteomic approach to quantify reversible oxidation of individual Cysteine residues revealed a relatively more oxidised redox state following exercise in theprdx-2mutants. Our results demonstrate that conserved cytoplasmic 2-Cys Peroxiredoxins are required for the beneficial adaptive response to a physiological stress.
- Published
- 2022