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203. Physico-chemical properties of hepatocyte plasma-membrane-bound arginase

Author

Jose M. Fuentes, Ml, Campo, and Soler G

Subjects
Hot Temperature, Arginase, Cations, Divalent, Cell Membrane, Rats, Inbred Strains, Cross Reactions, Hydrogen-Ion Concentration, Rats, Substrate Specificity, Isoenzymes, Kinetics, Liver, Enzyme Stability, Animals, Protein Binding
Abstract

Rat liver plasma-membrane-bound arginase was investigated in order to obtain data regarding its physico-chemical properties. Arginase bound to plasma membrane presented a specific activity of 0.74 +/- 0.09 IU/mg for the fully-activated enzyme, the pH of maximum activity being 9.8. Maximum stability was recorded at two pH values, 7 and 10.5 respectively. Mn2+ activated the enzyme, while Cu2+ and Zn2+, and to a lesser extend Co2+, showed a strong inhibitory effect. Ca2+ and Mg2+ had no effect at the concentrations assayed. The influence of temperature was studied in the presence and in the absence of Mn2+. The enzyme was stable up to 65 degrees C in both cases. Membrane- bound arginase showed an activation energy of 11.5 +/- 1.4 Kcal/mol between 20 and 40 degrees C, and 13.3 +/- 2.5 Kcal/mol between 40 and 60 degrees C. The Q10 for the same temperature ranges were 1.78 and 1.9 respectively. The membrane-bound enzyme presented two different Michaelis constants, one with high affinity (2.05 +/- 0.73 mM) and the other with low affinity for arginine (130 +/- 27.2 mM). Solubilized arginase showed very similar values. Among all the structural analogous assayed, only L-canavanine proved to be substrate for arginase, with and L-arginine/L-canavanine hydrolysis ratio of 5.8 +/- 0.28. No reactivity was found between plasma-membrane-bound arginase and anti-rat liver arginase antibodies raised in rabbits.

Published
1991

211. Randomized trial of adjuvant chemotherapy with mitomycin plus ftorafur versus mitomycin alone in resected locally advanced gastric cancer.

Author

Grau, J J, primary, Estapé, J, additional, Fuster, J, additional, Filella, X, additional, Visa, J, additional, Terés, J, additional, Soler, G, additional, Albiol, S, additional, García-Valdecasas, J C, additional, Grande, L, additional, Bombí, J, additional, Bordas, J, additional, and Alcobendas, F, additional

Published
1998
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224. Application of multiplex PCR approaches for shark molecular identification: feasibility and applications for fisheries management and conservation in the Eastern Tropical Pacific.

Author

CABALLERO, S., CARDEÑOSA, D., SOLER, G., and HYDE, J.

Subjects
POLYMERASE chain reaction, SHARKS, AGAROSE, TISSUES, ALOPIAS
Abstract

Here we describe the application of new and existing multiplex PCR methodologies for shark species molecular identification. Four multiplex systems (group ID, thresher sharks, hammerhead sharks and miscellaneous shark) were employed with primers previously described and some designed in this study, which allow for species identification after running PCR products through an agarose gel. This system was implemented for samples (bodies and fins) collected from unidentified sharks landed in the port of Buenaventura and from confiscated tissues obtained from illegal fishing around the Malpelo Island Marine Protected Area, Pacific Coast of Colombia. This method has allowed reliable identification, to date, of 407 samples to the genus and/or species levels, most of them (380) identified as the pelagic thresher shark ( Alopias pelagicus). Another seven samples were identified as scalloped hammerhead sharks ( Sphyrna lewini). This is an easy-to-implement and reliable identification method that could even be used locally to monitor shark captures in the main fishing ports of developed and developing countries. [ABSTRACT FROM AUTHOR]

Published
2012
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226. Lithium inhibits caspase 3 activation and dephosphorylation of PKB and GSK3 induced by K[sup +] deprivation in cerebellar granule cells.

Author

Mora, A., Sabio, G., González-Polo, R.A., Cuenda, A., Alessi, D.R., Alonso, J., Fuentes, J.M., Soler, G., and Centeno, F.

Subjects
LITHIUM, CEREBELLUM, APOPTOSIS, GLYCOGEN synthesis, PHYSIOLOGY
Abstract

Lithium protects cerebellar granule cells from apoptosis induced by low potassium, and also from other apoptotic stimuli. However, the precise mechanism by which this occurs is not understood. When cerebellar granule cells were switched to low potassium medium, the activation of caspase 3 was detected within 6 h, suggesting a role of caspase 3 in mediating apoptosis under conditions of low potassium. In the same conditions, lithium (5 mm) inhibited the activation of caspase 3 induced by low potassium. As lithium did not inhibit caspase 3 activity in vitro, these results suggest that this ion inhibits an upstream component that is required for caspase 3 activation. Lithium is known to inhibit a kinase termed glycogen sythase kinase 3 (GSK3), which is implicated in the survival pathway of phosphatidylinositol 3-kinase/protein kinase B (PI3K/PKB). Here we demonstrate that low potassium in the absence of lithium induces the dephosphorylation, and therefore the activation, of GSK3. However, when lithium was present, GSK3 remained phosphorylated at the same level as observed under conditions of high potassium. Low potassium induced the dephosphorylation and inactivation of PKB, whereas when lithium was present PKB was not dephosphorylated. Our results allow us to propose a new hypothesis about the action mechanism of lithium, this ion could inhibit a serine-threonine phosphatase induced by potassium deprivation. [ABSTRACT FROM AUTHOR]

Published
2001
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229. Diagnostic performance of arginase activity in colorectal cancer

Author

del Ara Rangel, M., González-Polo, R. A., Caro, A., del Amo, E., Palomo, L., Hernández, E., Soler, G., and Fuentes, J. M.

Abstract

Abstract: Arginase activity was measured in serum and biopsy from healthy individuals and colorectal cancer patients. Arginase activity in tumor samples (87�7.7 U/g tissue) was significantly higher than in controls (40.7�3.3 U/g tissue). However, serum arginase activity did not show any significant change in both groups. Finally, the micromethod used to quantify arginase activity in this study is superior to other methods because it has increased sensitivity, requires less sample, and is less time-consuming. Arginase differences are significant, according to the t-test (P<0.05).

Published
2002
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230. LRRFIP1, a new FGFR1 partner gene associated with 8p11 myeloproliferative syndrome.

Author

Soler, G., Nusbaum, S., Varet, B., Macintyre, E. A., Vekemans, M., Romana, S. P., and Radford-Weiss, I.

Subjects
*LETTERS to the editor, *GENES, *AMINO acids, *CHROMOSOMES, *DNA probes, *DOCUMENTATION, *KARYOTYPES, *MYELOPROLIFERATIVE neoplasms, *NUCLEOTIDES, *POLYMERASE chain reaction, *PROTEINS, *REVERSE transcriptase polymerase chain reaction
Abstract

A letter to the editor is presented discussing hematological malignancies associated with FGFR1 rearrangements.

Published
2009
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231. Fusion of ZMIZ1 to ABL1 in a B-cell acute lymphoblastic leukaemia with a t(9;10)(q34;q22.3) translocation.

Author

Soler, G., Radford-Weiss, I., Ben-abdelali, R., Mahlaoui, N., Ponceau, J. F., Macintyre, E. A., Vekemans, M., Bernard, O. A., and Romana, S. P.

Subjects
*LETTERS to the editor, *LYMPHOBLASTIC leukemia
Abstract

A letter to the editor is presented in response to the article "Fusion of ZMIZ1 to ABL1 in a B-cell Acute Lymphoblastic Leukaemia With a t(9;10)(q34;q22.3) Translocation," in the November 15, 2007 issue.

Published
2008
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233. Anti-MHV3 state induced by IFN gamma in macrophages is not related to arginine metabolism

Author

Pereira, C. A., Soler, G., and Modolell, M.

Abstract

In contrast to BALB/c mouse macrophages, the A/J macrophages after activation by interferon gamma (IFN gamma) develop an anti-MHV3 effect which correlates with the resistance to virus infection. To understand the cellular basis of this antiviral effect, we studied the possible involvement of arginine metabolism through nitric oxide (NO) and arginase induction, since these metabolic pathways have been described as implicated in antiviral activities of macrophages. The studies were performed by activating macrophages with inducers of NO (IFN gamma) and arginase (IL4 IL10). NO synthase (iNOS) and arginase inhibitors (N-methyl-arginine, NMA, and hydroxyarginine, OH-ARG) were used. The results show that in both macrophage populations, no spontaneous synthesis of NO occurred and the MHV3 enhanced the NO release induced by IFN gamma. After activation with IFN gamma, BALB/c macrophages released higher amounts of NO than the A/J macrophages. The inhibition of IFN gamma-induced NO-synthesis with NMA or with arginine free medium did not affect the virus replication. In BALB/c macrophages, IL4 or IL10, induced higher amounts of arginase than in A/J macrophages. In both macrophage populations the MHV3 infection had no influence on the arginase synthesized, and the inhibition of the arginase with OH-ARG had no influence on the virus growth. The level of MHV3 replication or inhibition was also not influenced when we used macrophages from knockout mice for the iNOS gene, and as a consequence were unable of synthesizing NO. These data indicate that NO and arginase do not participate in the anti-MHV3 state induced by IFN gamma in macrophages.

Published
1997
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234. An arginine regulated γ-guanidobutyrate ureahydrolase from tench liver (Tinca tinca L.)

Author

Campo, M. L., Fuentes, J. M., and Soler, G.

Abstract

A γ-guanidobutyrate ureahydrolase isolated from tench liver has been characterized. Some of its physico-chemical properties like pH effect and thermal stability resemble those of arginases, however it shows some peculiarities that makes it different from arginases and other amidino hydrolases. Thus cation requirement is not as strong as in arginases, and the Km value for γ-guanido-butyric acid (230 ± 25 mM) is shifted to a lower value (45 ± 5 mM) by 5 mM arginine. The possible regulatory role of arginine on γ-guanidobutyrate ureahydrolase activity is discussed.

Published
1992
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235. Kinetics and inhibition by some aminoacids of lactating rat mammary gland arginase

Author

Fuentes, J. M., Campo, M. L., and Soler, G.

Abstract

Some kinetic and regulatory properties of lactating rat mammary gland arginase were studied. At pH 7.4, i.e. at near-physiological conditions, there was evidence of inhibition by excess of substrate, with a Km value of 9.5 mM, slightly lower than the value of 18 mM observed at pH 9.8 (maximum enzyme activity). A study was also made of the effects of proline, ornithine, lysine and certain branched-chain aminoacids on enzyme activity: lactating rat mammary gland arginase was strongly and competitively inhibited by lysine, ornithine and valine, with Ki values of 1.2 mM, 1.1 mM and 3.6 mM, respectively. Other aminoacids (proline, isoleucine and leucine) also inhibited lactating rat mammary gland arginase, although to a lesser extent.

Published
1994
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236. Oscillations in rat liver cytosolic enzyme activities of the urea cycle

Author

Fuentes, J. M., Pascual, M. R., Salido, G., Soler, G., and Madrid, J. A.

Abstract

Diurnal rhythms were studied in three rat liver enzymes of the urea cycle: arginase, arginosuccinate synthetase and arginosuccinase. In animals synchronized to a 12:12 h light-dark cycle these enzymes were determined at 8 different time points under three different feeding schedules: 24 h of fasting, ad libitum feeding and restricted feeding. Under the three experimental conditions maxima of enzyme activities occured during the dark period. In all cases the maximum activity of arginosuccinase preceded the one of arginase and these in turn the one of arginosuccinate synthetase. On the other hand, the hepatic protein level was maximal during the light period and decreased to its lowest level during the dark period. The restriction of food between 08.00 h and 14.00 h induced an important phase shift of hepatic protein rhythm and arginosuccinase activity.Our results suggest that the diurnal rhythms of cytosolic enzymes of the urea cycle are not only dependent on the light-dark cycle, but also on the synchronizing and masking effect of food intake.

Published
1994
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237. FIV en ciclo natural: del origen de la FIV a la práctica asistencial actual

Author

Carrillo Torres, P., Goday Cibeira, A., Hernández Dacruz, B., and Casals Soler, G.

Abstract

El principio básico de la fecundación in vitro en ciclo natural (FIV-CN) recae en el reclutamiento mediante la selección folicular espontánea, minimizando la manipulación del ciclo de la mujer. Recientemente esta técnica ha suscitado mayor interés y se han puesto en valor muchas de sus ventajas respecto a la FIV convencional (FIVc). FIV-CN y FIVc son tratamientos complementarios que permiten ampliar el espectro terapéutico en las parejas con indicación de FIV. La FIV-CN constituye un tratamiento a considerar especialmente en la paciente con mal pronóstico para la FIVc por baja reserva ovárica. La edad es su principal factor pronóstico y los resultados son aceptables hasta los 40 años. El principal factor limitante de la FIV-CN es el riesgo de cancelación por ovulación, que puede reducirse mediante diferentes estrategias. No obstante, los resultados por transferencia embrionaria son satisfactorios en diferentes series publicadas.

Published
2021
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238. Ciudad de Montevideo [Material cartográfico] : plano topográfico de la ciudad de Montevideo en la época de la independencia, año 1811

Author

Soler, G., fl. ca. 1811, Uruguay. Dirección de Topografía, and Reunión Panamericana de Consulta sobre Cartografía (4ª: 1948: Buenos Aires)

Subjects
Montevideo
Abstract

Coordenadas de la ciudad de Montevideo referidas al meridiano de Buenos Aires (E 2º 00' 03'' / S 34º 54' 48''). Orientado con rosa de ocho vientos Relación de los edificios civiles y militares así como de los baluartes indicados por clave alfabética y numérica Nota explicativa que informa de la naturaleza del documento, con datos históricos de la ciudad representada En el margen inferior "Recuerdo de la IV Reunión Panamericana de consulta sobre Cartografía, realizada en Buenos Aires del 15 de octubre al 14 de noviembre de 1948" Copia digital. Madrid : Ministerio de Cultura. Dirección General del Libro, Archivos y Bibliotecas, 2010

Published
1948

240. Obesity decreases clinical efficacy and levels of adalimumab in patients with ankylosing spondylitis

Author

Rosas J, Llinares-Tello F, José Miguel Senabre Gallego, Barber-Vallés X, Santos-Soler G, Salas-Heredia E, Pons Bas A, Cano Pérez C, García-Carrasco M, and Aire-Mb, And Group

Subjects
adalimumab, anti-adalimumab antibody, ankylosing spondylitis, body mass index, human activities
Abstract

Objective. Obesity can be a factor that affects response to anti-TNF drugs. However, studies on patients with ankylosing spondylitis (AS) are rare. We aimed to determine whether obesity affects serum levels of adalimumab (ADL), and immunogenicity and clinical efficacy of the drug in patients with AS. Methods. A cross-sectional study on 57 patients with axial AS receiving ADL was conducted. They received DMARD per standard of care at their rheumatologist's discretion. Patients' body mass index (BMI) was obtained when ADL treatment began. Clinical response was evaluated using the Spanish versions of the BASDAI index and the ASDAS ESR index. Serum concentrations of free ADL (trough level) and anti-ADL antibodies were measured using Promonitor-ADL and Promonitor Anti-ADL ELISA kits (Progenika Grifols SA, Spain), just prior to the next subcutaneous injection of ADL. Results. Patients with BMI >30 kgl nt2 (obese) as opposed to BMI

242. Fibromyalgia prevalence and related factors in a large registry of patients with systemic lupus erythematosus

Author

Torrente-Segarra, V., Salman-Monte, T. C., Rúa-Figueroa, Í, Pérez-Vicente, S., López-Longo, F. J., Galindo-Izquierdo, M., Calvo-Alén, J., Olivé-Marqués, A., Ibáñez-Ruán, J., Horcada, L., Sánchez-Atrio, A., Montilla, C., Rodríguez-Gómez, M., Díez-Álvarez, E., Martinez-Taboada, V., Andreu, J. L., Fernández-Berrizbeitia, O., Hernández-Beriain, J. A., Gantes, M., Hernández-Cruz, B., Pecondón-Español, A., Marras, C., Bonilla, G., José María Pego-Reigosa, Blanco, R., Vela-Casasempere, P., Melero-González, R., Otón-Sánchez, T., Tornero-Muriel, E., Uriarte-Isacelaya, E., Freire-González, M., Fito-Manteca, M. C., Fernández-Nebro, A., Narváez, J., Zea-Mendoza, A., Rosas-Gómez Salazar, J. C., Ibáñez-Barceló, M., Pérez-Venegas, J. J., La Torre Ortega, I., Pérez, L. C., Delgado, P. C., Rodríguez-Almaraz, E., Heredia, E. S., Soler, G. S., Ramírez, C. S., Senabre Gallego, J. M., Collado, M. A., Bernal, J. A., Vilamajó, I. R., Mas, A. J., Murillo, C., Barranco, I. C., Melchor, E. G., Fontova, J. C., Manrique, M. G., Lencastre, C. G., Martínez-Losa, M. M., Almagro, R. M., González-Gay Mantecón, M. A., Martíny, I. P., Del Carmen Bejerano, M., Blanco, I. V., Moreira, B., Aurrecoechea, E., Jimeno, T. R., Zamorano, A. A., Magro, C., Álvarez, E. R., Arruabarrena, C. E., Mérida, M. A. A., Egües Dubuc, C. A., Fanlo, J. C., Álvarez, E. D., Vitovi, C., Robles, A. L., Rodríguez, T. V., Oyarzábal, M. V. I., López, M. A. B., Romero Barco, C. M., López, J. A. M., Pernaute, O. S., Vicuña Pinedo, T. G., Expósito, M. V., La Peña, P. G., Rubio, S. R., González Martín, J. J., Gómez, A. P., Bohórquez, C., Hajkhan, A. M., Turrión Nieves, A. I., Lois Iglesias, A. J., Boteanu, A. L., Gamir, M. L. G., Alberti, P. R., Fernández, S. M., Oliva, M. R., Stoye, C., Rodríguez, I. H., Rodríguez, C. M., Aspe La Iglesia, B., González, R. L., Sarabia, F. N., Toyos Sáenz Miera, F. J., La Fuente, J. L. M., Montañés, J. U., Sánchez, R. H., Pérez, R. M., Lozano, B. R., Angulo, E. U., Lucea, M. E. R., Domínguez, L. L., Alegre Sancho, J. J., La Morena Barrio, I., Valls, E., Ruiz, J. M., Quevedo Vila, V. E., Machín, S., Nóvoa, J., and Fernández, L. S.

244. Clinical relevance of monitoring serum levels of adalimumab in patients with rheumatoid arthritis in daily practice

Author

Rosas J, Llinares-Tello F, de la Torre I, Santos-Ramírez C, José Miguel Senabre Gallego, Valor L, Barber-Vallés X, Hernández-Flórez D, Santos-Soler G, Salas-Heredia E, Carreño L, and Aire-Mb, Group

Subjects
musculoskeletal diseases, skin and connective tissue diseases, human activities, humanities
Abstract

The aim of this paper is to assess the usefulness of measuring serum levels of adalimumab (ADL) and anti-ADL antibodies in 57 patients with rheumatoid arthritis (RA) treated with ADL for at least 3 months in daily practice.

246. Juvenile- and adult-onset systemic lupus erythematosus: A comparative study in a large cohort from the Spanish Society of Rheumatology Lupus Registry (RELESSER)

Author

Torrente-Segarra, V., Salman-Monte, T. C., Rúa-Figueroa, Í, Sánchez-Alonso, F., López-Longo, F. J., Galindo-Izquierdo, M., Calvo-Alén, J., Olivé-Marqués, A., Ibañez-Ruán, J., Horcada, L., Sánchez-Atrio, A., Montilla, C., Melero-González, R. B., Díez-Álvarez, E., Martinez-Taboada, V., Andreu, J. L., Fernández-Berrizbeitia, O., Hernández-Beriain, J., Gantes, M., Hernández-Cruz, B., Pecondón-Español, Á, Carlos Marras, Bonilla, G., Pego-Reigosa, J. M., Blanco, R., Vela-Casasempere, P., Melero-González, R., Otón-Sánchez, T., Tornero-Muriel, E., Uriarte-Isacelaya, E., Freire-González, M., Fito-Manteca, M. C., Fernández-Nebro, A., Narváez, J., Zea-Mendoza, A., Salazar, J. C. R. -G, Ibáñez-Barceló, M., Pérez-Venegas, J. J., Ortega, I. T., Pérez, L. C., Delgado, P. C., Rodríguez-Almaraz, E., Heredia, E. S., Soler, G. S., Ramírez, C. S., Gallego, J. M. S., Collado, M. A., Bernal, J. A., Vilamajó, I. R., Mas, A. J., Murillo, C., Barranco, I. C., Grados, D., Fontova, J. C., Manrique, M. G., Lencastre, C. G., Martínez-Losa, M. M., Almagro, R. M., Mantecón, M. A. G. -G, Martíny, I. P., Bejerano, M. C., Blanco, I. V., Moreira, B., Aurrecoechea, E., Jimeno, T. R., Zamorano, Á A., Magro, C., Álvarez, E. R., Arruabarrena, C. E., Mérida, M. Á A., Dubuc, C. A. E., Fanlo, J. C., Álvarez, E. D., Vitovi, C., Robles, A. L., Rodríguez, T. V., Oyarzábal, M. V. I., López, M. Á B., Barco, C. M. R., López, J. A. M., Pernaute, O. S., Pinedo, T. G. V., Expósito, M. V., La Peña, P. G., Rubio, S. R., Martín, J. J. G., Gómez, A. P., Bohórquez, C., Hajkhan, A. M., Nieves, A. I. T., Iglesias, A. J. L., Boteanu, A. L., Gamir, M. L. G., Alberti, P. R., Fernández, S. M., Oliva, M. R., Stoye, C., Rodríguez, Í H., Rodríguez, C. M., La Iglesia, B. A., González, R. L., Sarabia, F. N., Miera, F. J. T. S., La Fuente, J. L. M., Montañés, J. U., Sánchez, R. H., Pérez, R. M., Lozano, B. R., Angulo, E. Ú, Lucea, M. E. R., Domínguez, L. L., Sancho, J. J. A., Barrio, I. L. M., Valls, E., Ruiz, J. M., Vila, V. E. Q., Machín, S., Nóvoa, J., and Fernández, L. S.

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