449 results on '"Tezuka N"'
Search Results
202. Spin Tunneling Magnetoresistive Effect In Ferromagnet/AI/sub 2/O/sub 3/Ferromagnet Junctions.
- Author
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Miyazaki, T. and Tezuka, N.
- Published
- 1995
- Full Text
- View/download PDF
203. Corrigendum: Substantial reduction of critical current for magnetization switching in an exchange-biased spin valve.
- Author
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Jiang, Y., Nozaki, T., Abe, S., Ochiai, T., Hirohata, A., Tezuka, N., and Inomata, K.
- Subjects
MAGNETIZATION - Abstract
A correction to the article about the substantial reduction of critical current for magnetization switching in an exchange-biased spin valve that was published in a previous issue is presented.
- Published
- 2005
- Full Text
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204. Reconstruction of the Aortic Arch in Invasive Thymoma Under Retrograde Cerebral Perfusion
- Author
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Fujino, S., Tezuka, N., Watarida, S., Katsuyama, K., Inoue, S., and Mori, A.
- Published
- 1998
- Full Text
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205. Giant magnetic tunneling effect in Fe/Al 2O 3/Fe junction
- Author
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Miyazaki, T. and Tezuka, N.
- Published
- 1995
- Full Text
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206. Correlation between amplitude of spin accumulation signals investigated by Hanle effect measurement and effective junction barrier height in CoFe/MgO/n{sup +}-Si junctions
- Author
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Tezuka, N. [Department of Materials Science, Graduate School of Engineering, Tohoku University, Sendai 980-8579 (Japan)]
- Published
- 2015
- Full Text
- View/download PDF
207. Local magnetoresistance through Si and its bias voltage dependence in ferromagnet/MgO/silicon-on-insulator lateral spin valves
- Author
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Tezuka, N. [Department of Materials Science, Graduate School of Engineering, Tohoku University, Sendai 980-8579 (Japan)]
- Published
- 2014
- Full Text
- View/download PDF
208. Maximum magnitude in bias-dependent spin accumulation signals of CoFe/MgO/Si on insulator devices
- Author
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Tezuka, N. [Department of Materials Science, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza-Aoba, Sendai, Miyagi 980-8579 (Japan)]
- Published
- 2013
- Full Text
- View/download PDF
209. Inhibition of cauliflower mosaic virus multiplication by actinomycin D
- Author
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Tezuka, N., Taniguchi, T., and Matsui, C.
- Published
- 1971
- Full Text
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210. Improvement of coercivity of anisotropic Nd–Fe–B HDDR powders by Ga addition
- Author
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Sugimoto, S., Murai, H., Koike, N., Nakamura, H., Book, D., Tezuka, N., Kagotani, T., Okada, M., Homma, M., and Inomata, K.
- Subjects
- *
METAL powders , *GALLIUM , *MAGNETICS , *ANISOTROPY - Abstract
The effect of Ga addition on the increase of coercivity of HDDR-treated Nd–Fe–B powders was investigated. The Ga addition suppresses grain growth and may decrease the region where magnetocrystalline anisotropy is reduced. In addition, a heat treatment in which the hydrogen pressure was decreased in steps during the recombination reaction resulted in good magnetic properties of
Br=1.44 T,jHc=0.97 MA m−1 and(BH)max=308 kJ m−3. [Copyright &y& Elsevier]- Published
- 2002
- Full Text
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211. ON THE CONSTRUCTION OF A HARBOR FOR THE FUKUSHIMA ATOMIC POWER STATION OF TOKYO POWER COMPANY.
- Author
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Tezuka, N
- Published
- 1969
212. Observation of the feeding behaviour of reared Japanese eel Anguilla japonica leptocephali fed picocyanobacteria Synechococcus spp.
- Author
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Kenzaki A, Okunishi S, Tomoda T, Shioura Y, Uchida M, Tezuka N, and Maeda H
- Subjects
- Animals, Feeding Behavior, Larva, Anguilla, Synechococcus
- Abstract
The authors observed the feeding behaviour of artificially reared Japanese eel Anguilla japonica leptocephali, 7.5-19 mm total length (10-61 days post-hatch), fed Synechococcus sp., which is considered a potential food source of anguilliform larvae. Three strains of Synechococcus sp. (NIES-972, 976 and 979) were tested as the food material. Larvae across the entire length range could effectively ingest a suspension of pico-sized cyanobacteria (1-3 μm in diameter). Video observations of the mid-hindgut of larvae under an epifluorescence microscope confirmed that the movement of microvilli of the intestinal epithelium allowed the cell particles to circulate in the mid-hindgut, before becoming solidified in the anal region. Significant differences in food intake were observed between larvae fed two strains of Synechococcus (NIES-972 and 976), and among different cell densities, which suggests feeding selectivity and density dependence. Comparisons of feeding behaviour under the light group (9L:15D) and the dark group (24D) showed significantly higher food intake (measured as an index of intestinal fullness) in the light group, although substantial and continuous ingestion was observed in the dark group, indicating continuous feeding by swallowing sea water. The authors hypothesise that the feeding ecology of anguilliform leptocephali is based on a survival strategy whereby the larvae do not compete with various higher-trophic-level fishes for food in an oligotrophic environment., (© 2021 Fisheries Society of the British Isles.)
- Published
- 2022
- Full Text
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213. Application of heavy-ion-beam irradiation to breeding large rotifer.
- Author
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Tsuneizumi K, Yamada M, Kim HJ, Ichida H, Ichinose K, Sakakura Y, Suga K, Hagiwara A, Kawata M, Katayama T, Tezuka N, Kobayashi T, Koiso M, and Abe T
- Subjects
- Animal Feed, Animals, Aquaculture, Larva growth & development, Larva radiation effects, Mutation, Radiation, Ionizing, Rotifera genetics, Rotifera growth & development, Rotifera physiology, Heavy Ions, Rotifera radiation effects
- Abstract
In larviculture facilities, rotifers are generally used as an initial food source, while a proper size of live feeds to connect rotifer and Artemia associated with fish larval growth is needed. The improper management of feed size and density induces mass mortality and abnormal development of fish larvae. To improve the survival and growth of target larvae, this study applied carbon and argon heavy-ion-beam irradiation in mutation breeding to select rotifer mutants with larger lorica sizes. The optimal irradiation conditions of heavy-ion beam were determined with lethality, reproductivity, mutant frequency, and morphometric characteristics. Among 56 large mutants, TYC78, TYC176, and TYA41 also showed active population growth. In conclusion, (1) heavy-ion-beam irradiation was defined as an efficient tool for mutagenesis of rotifers and (2) the aforementioned 3 lines that have larger lorica length and active population growth may be used as a countermeasure of live feed size gap during fish larviculcure., (© The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
- Published
- 2021
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214. [Clinical Efficacy of Monotherapy with Immune Checkpoint Inhibitors for Advanced Non-Small Cell Lung Cancer].
- Author
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Sasaki T, Tabata T, Nonomura R, Oshima Y, Mitomo H, Ishibashi N, Sugawara T, Tezuka N, Sagawa M, Kondo T, and Yoshimura N
- Subjects
- Humans, Neoplasm Recurrence, Local, Retrospective Studies, Treatment Outcome, Antineoplastic Agents, Immunological therapeutic use, Carcinoma, Non-Small-Cell Lung drug therapy, Lung Neoplasms drug therapy
- Abstract
Objective: To retrospectively assess data on immune checkpoint inhibitors(ICIs)in an actual clinical setting, examine the factors that contribute to response and survival using real-world data, and compare the effectiveness of the 3 types of ICIs for patients with non-small cell lung cancer(NSCLC)., Methods: A retrospective analysis of 127 patients with NSCLC treated with ICIs at our hospital was conducted., Results: Nivolumab(56 patients)showed a 3-year survival rate of 21.6% and a disease control rate of 57.1%. These results are consistent with the clinical trials of Nivolumab. Pembrolizumab(36 patients) showed a 2-year survival rate of 60.3%, a response rate of 50.0%, and a disease control rate of 63.9%. Atezolizumab(35 patients)displayed a particularly low response rate with a 1-year survival rate of 58.4%, response rate of 8.6%, and disease control rate of 25.7%. The treatment results for recurrence after surgery for lung cancer were comparable to those for unresectable lung cancer., Conclusion: Anti-PD-1 antibody displayed better therapeutic results than anti-PD-L1 antibody. The efficacy of ICI administration for postoperative recurrent lung cancer was also shown in this study.
- Published
- 2020
215. Lipshutz-type bis(amido)argentates for directed ortho argentation.
- Author
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Tezuka N, Hirano K, Peel AJ, Wheatley AEH, Miyamoto K, and Uchiyama M
- Abstract
Bis(amido)argentate (TMP)
2 Ag(CN)Li2 ( 3 , TMP-Ag-ate; TMP = 2,2,6,6-tetramethylpiperidido) was designed as a tool for chemoselective aromatic functionalization via unprecedented directed ortho argentation (D o Ag). X-Ray crystallographic analysis showed that 3 takes a structure analogous to that of the corresponding Lipshutz cuprate. D o Ag with this TMP-Ag-ate afforded multifunctional aromatics in high yields in processes that exhibited high chemoselectivity and compatibility with a wide range of functional groups. These included organometallics- and transition metal-susceptible substituents such as methyl ester, aldehyde, vinyl, iodo, (trifluoromethanesulfonyl)oxy and nitro groups. The arylargentates displayed good reactivity with various electrophiles. Chalcogen (S, Se, and Te) installation and azo coupling reactions also proceeded efficiently., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)- Published
- 2020
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216. Terahertz wave generation via difference frequency generation using 2D In x Ga 1-x Se crystal grown from indium flux.
- Author
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Sato Y, Tang C, Watanabe K, Ohsaki J, Yamamoto T, Tezuka N, Tanabe T, and Oyama Y
- Abstract
We demonstrate the generation of THz waves (frequency 9.7 THz) using difference frequency generation in an In
x Ga1-x Se mixed crystal grown from In flux. The amount of indium and the lattice constant of the crystal were evaluated using electron micro probe analysis and X-ray diffraction, respectively. We believe that the Ga sites were substituted by In atoms in the Inx Ga1-x Se crystal because the In content, estimated according to the Vegard's law, was similar to that measured by EPMA. The maximum power of the generated THz wave was 39 pJ and the conversion efficiency was 1.7×10-5 J-1 . This conversion efficiency was 28 times larger than that reported for undoped GaSe crystal.- Published
- 2020
- Full Text
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217. A Direct, Chemo-, and Regioselective Cross-Coupling Reaction of Arenes via Sequential Directed ortho Cuprations and Oxidation.
- Author
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Tezuka N, Hirano K, and Uchiyama M
- Abstract
Direct cross-coupling of Csp
2 -H/Csp2 -H bonds of two arenes was achieved in 30-76% yield via sequential directed ortho cuprations (D o Cu) with the cuprate base (TMP)2 Cu(CN)Li2 ( 1 , TMP = 2,2,6,6-tetramethylpiperidido), followed by oxidation. This methodology offers easy access to unsymmetric biaryls from arenes with a variety of directed metalation groups (DMGs) and ancillary functional groups, taking advantage of the highly chemoselective action of 1 .- Published
- 2019
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218. A new route for the efficient metalation of unfunctionalized aromatics.
- Author
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Peel AJ, Tezuka N, D'Rozario JM, Uchiyama M, and Wheatley AEH
- Abstract
The synthesis and isolation of a novel bimetallic species formed by reacting two equivalents of TMPLi with CuCl in the presence of Et
2 O are reported. X-ray crystallography reveals the Et2 O-free tetranuclear aggregate (TMPCu)2 (TMPLi)2 1 , which formally results from the catenation of dimers of TMPLi and TMPCu. NMR spectroscopy confirms that, upon dissolution in hydrocarbon media, the crystals fail to form a conventional Gilman cuprate dimer. Instead they exhibit a spectrum which is consistent with that recently proposed for an isomer of dimeric Gilman cuprate. Moreover, while pre-isolated Gilman cuprate is inert to benzene solvent, this new isomer smoothly affects aromatic deprotonation to give mainly Ph(TMP)3 Cu2 Li2 3 , which is formally a heterodimer of Gilman cuprate TMPCu(μ-TMP)Li 2 and PhCu(μ-TMP)Li 4 . Attempts to synthesise 3 through explicit combination of pre-isolated 2 and 4 were successful; additionally, this permitted the preparation of Ph(TMP)3 Cu3 Li 5 and Ph(TMP)3 CuLi3 7 when 4 was combined in 1 : 2 ratios with TMPCu or TMPLi, respectively. 5 was characterised as metallacyclic in the solid-state, its structural features resembling those in 3 but with reduced Li-π interactions. It also proved possible to perform Cu/Li exchange on 5 (usingt BuOCu) to give a novel mixed organo(amido)copper species Ph(TMP)3 Cu4 6 . Remarkably, the unprecedented reactivity of 1 towards benzene is reproduced by heating a 1 : 1 mixture of TMPLi and TMPCu in the same solvent; this gives predominantly 3 . On the other hand, mixtures which are rich in either Cu or Li can lead to the selective in situ formation of 5 or 7 . Though crystallographic data on 7 could not be obtained, DFT calculations accurately corroborated the observed structures of 3 and 5 and could be used to support 7 having the same structure type, albeit with enhanced Li-π interactions. This was consistent with NMR spectroscopic data. However, in contrast to 3 and 5 , for which 2D NMR spectroscopy indicated only conformational changes, 7 was additionally found to exhibit fluxionality in a manner consistent with a dissociative process.- Published
- 2019
- Full Text
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219. Clinical outcome of posterior fixation surgery in patients with vertebral metastasis of lung cancer.
- Author
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Igarashi T, Okamoto K, Teramoto K, Kaku R, Ishida K, Ueda K, Kawaguchi Y, Hori T, Hashimoto M, Kitamura S, Tezuka N, and Hanaoka J
- Abstract
Vertebral metastasis of non-small-cell lung cancer (NSCLC) often leads to neurological paralysis, with deterioration of the patients' activities of daily living (ADL). Surgical treatments for the symptoms are unlikely to be recommended due to the poor prognosis of patients with advanced NSCLC. The aim of the present study was to retrospectively evaluate the clinical outcome of posterior spinal fixation surgery in patients with neurological paralysis resulting from vertebral metastasis of NSCLC. Between April, 2007 and March, 2012, 4 patients (3 men and 1 woman; median age, 56.5 years) underwent fixation surgery at the Shiga University of Medical Science Hospital (Otsu, Japan). The mean preoperative Tokuhashi and Tomita scores of the patients were high (8.25 and 7.0, respectively). However, the Frankel grade functional score and performance status of the patients improved following fixation surgery, after which all patients received chemoradiotherapy. Postoperatively, the median paralysis-free time was 41 months (range, 17-42 months) and the median survival time was 42.5 months (range, 22-43 months). According to the functional scores, the patients had a poor prognosis, which may have been a contraindication for fixation surgery. In these cases, however, surgical treatment improved the patients' ADL and increased the likelihood of receiving anticancer therapy, contributing to the prolongation of survival. Therefore, fixation surgery may be beneficial for patients with neurological paralysis following vertebral metastasis of advanced NSCLC.
- Published
- 2017
- Full Text
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220. Simultaneous resection of pulmonary tumor following cardiovascular surgery.
- Author
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Kaku R, Teramoto K, Ishida K, Igarashi T, Hashimoto M, Kitamura S, Tezuka N, Asai T, and Hanaoka J
- Subjects
- Aged, Aged, 80 and over, Cardiac Surgical Procedures methods, Cardiac Surgical Procedures mortality, Cardiovascular Diseases diagnosis, Cohort Studies, Combined Modality Therapy, Comorbidity, Disease-Free Survival, Female, Humans, Japan, Kaplan-Meier Estimate, Lung Neoplasms pathology, Male, Middle Aged, Pneumonectomy mortality, Prognosis, Retrospective Studies, Statistics, Nonparametric, Survival Analysis, Thoracotomy methods, Treatment Outcome, Cardiovascular Diseases epidemiology, Cardiovascular Diseases surgery, Hospital Mortality, Lung Neoplasms epidemiology, Lung Neoplasms surgery, Pneumonectomy methods
- Abstract
Background: A pulmonary tumor is occasionally detected on a chest computed tomography (CT) scan before cardiovascular surgery., Purpose: In this study, we examined clinical courses of patients who had undergone the simultaneous resection of a pulmonary tumor following cardiovascular surgery., Methods: From 2008 to 2013, 18 patients (13 men and 5 women) with a median age of 69.8 years underwent the wedge pulmonary resection for a lung tumor through a median thoracotomy following cardiovascular surgery in our hospital. Cardiovascular surgeries consisted of off-pump coronary artery bypass grafting (CABG) in six patients, aortic valve replacement and/or mitral valve plasty in 10 patients, total arch replacement in 10 patients and descending aorta replacement in 10 patients., Results: No complications associated with pulmonary resections were observed. Pathological examination revealed that 15 patients (83.3%) were diagnosed with lung cancers including 13 adenocarcinomas and two squamous cell carcinomas, with the clinical stages of 1A in 13 patients, 2A in one patient and 2B in one patient. Among them, five patients received the radical pulmonary resection subsequently, whereas 10 patients were unable to receive it due to their poor cardiopulmonary function. Kaplan-Meier analysis of patients with lung cancer revealed that the 5-year survival rate and progression-free survival (PFS) rate after 3 years from the surgery were 46.2% and 73.8%, respectively., Conclusion: The simultaneous resection of pulmonary tumor following cardiovascular surgery is safely performed, and is useful for the pathological diagnosis of the tumor. Further studies are warranted, however, this procedure may contribute to controlling the progression of lung cancer in patients with cardiovascular disease with comorbidities., (Copyright © 2017. Published by Elsevier Taiwan.)
- Published
- 2017
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221. Predictive biomarkers and effectiveness of MUC1-targeted dendritic-cell-based vaccine in patients with refractory non-small cell lung cancer.
- Author
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Teramoto K, Ozaki Y, Hanaoka J, Sawai S, Tezuka N, Fujino S, Daigo Y, and Kontani K
- Abstract
Background: The dendritic cell (DC)-based vaccine targeting the highly immunogenic tumor antigen, MUC1, has been promising for a cancer immunotherapy; however, predictive biomarkers for beneficial clinical responses of the vaccine remain to be determined., Methods: DCs loaded with MUC1-derived peptide were subcutaneously administered to patients with MUC1-positive non-small cell lung cancer (NSCLC) that was refractory to standard anticancer therapies, every 2 weeks. The effectiveness and tolerability of the vaccine were evaluated, and predictive biomarkers of clinical responses were explored., Results: Between August 2005 and May 2015, 40 patients received the vaccines. The median survival time (MST) after the initial vaccination was 7.4 months, and the 1-year survival rate was 25.0%. The MST for patients who received more than six vaccinations was 9.5 months, and the 1-year survival rate was 39.3%. In this cohort, patients who experienced immune-related adverse events, including skin reactions at the vaccination site and fever, had significantly longer survival times compared with patients without those immune-related adverse events (12.6 versus 6.7 months, p = 0.042). Longer survival times were also observed in patients whose peripheral white blood cells contained >20.0% lymphocytes (12.6 versus 4.5 months; p = 0.014). MUC1-specific cytotoxic immune responses were achieved in all of seven patients analyzed who received six vaccinations., Conclusion: The MUC1-targeted DC-based vaccine induced an antitumor immune response that promoted prolonged survival of patients with refractory NSCLC. The occurrence of immune-related adverse events and having a higher percentage of peripheral lymphocytes were predictive biomarkers of a beneficial clinical response during cancer immunotherapy for NSCLC., Competing Interests: Conflict of interest statement: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2017
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222. Direct Hydroxylation and Amination of Arenes via Deprotonative Cupration.
- Author
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Tezuka N, Shimojo K, Hirano K, Komagawa S, Yoshida K, Wang C, Miyamoto K, Saito T, Takita R, and Uchiyama M
- Abstract
Deprotonative directed ortho cupration of aromatic/heteroaromatic C-H bond and subsequent oxidation with t-BuOOH furnished functionalized phenols in high yields with high regio- and chemoselectivity. DFT calculations revealed that this hydroxylation reaction proceeds via a copper (I → III → I) redox mechanism. Application of this reaction to aromatic C-H amination using BnONH2 efficiently afforded the corresponding primary anilines. These reactions show broad scope and good functional group compatibility. Catalytic versions of these transformations are also demonstrated.
- Published
- 2016
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223. MRI-related thermal injury due to skin-to-skin contact.
- Author
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Takahashi T, Fujimoto N, Hamada Y, Tezuka N, and Tanaka T
- Subjects
- Aged, Humans, Magnetic Resonance Imaging instrumentation, Male, Burns etiology, Leg Injuries etiology, Magnetic Resonance Imaging adverse effects
- Published
- 2016
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224. Rhodium-Catalyzed (Perfluoroalkyl)olefination of Acetanilides Leading to Perfluoroalkylated Aromatics.
- Author
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Harada K, Tezuka N, Hirano K, Miyamoto K, Saito T, and Uchiyama M
- Subjects
- Catalysis, Fluorocarbons chemistry, Hydrocarbons, Aromatic chemistry, Molecular Structure, Acetanilides chemistry, Alkenes chemistry, Fluorocarbons chemical synthesis, Hydrocarbons, Aromatic chemical synthesis, Rhodium chemistry
- Abstract
We have developed an efficient Rh-catalyzed (perfluoroalkyl)olefination reaction of acetanilides, which provides a versatile synthetic entry to a range of perfluoroalkylated compounds.
- Published
- 2016
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225. "Cation-Stitching Cascade": exquisite control of terpene cyclization in cyclooctatin biosynthesis.
- Author
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Sato H, Teramoto K, Masumoto Y, Tezuka N, Sakai K, Ueda S, Totsuka Y, Shinada T, Nishiyama M, Wang C, Kuzuyama T, and Uchiyama M
- Subjects
- Cations chemistry, Cyclization, Diterpenes chemistry, Isotope Labeling, Stereoisomerism, Terpenes metabolism, Thermodynamics, Diterpenes metabolism, Terpenes chemistry
- Abstract
Terpene cyclization is orchestrated by terpene cyclases, which are involved in the biosynthesis of various cyclic natural products, but understanding the origin and mechanism of the selectivity of terpene cyclization is challenging. In this work, we describe an in-depth mechanistic study on cyclooctatin biosynthesis by means of theoretical calculations combined with experimental methods. We show that the main framework of cyclooctatin is formed through domino-type carbocation transportation along the terpene chain, which we call a "cation-stitching cascade", including multiple hydrogen-shifts and a ring rearrangement that elegantly determine the stereoselectivity.
- Published
- 2015
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226. Rocuronium and sugammadex under TOF monitoring on mECT.
- Author
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Hamaguchi S, Tezuka N, and Nagao M
- Subjects
- Female, Humans, Middle Aged, Rocuronium, Sugammadex, Androstanols administration & dosage, Electroconvulsive Therapy methods, gamma-Cyclodextrins administration & dosage
- Published
- 2015
- Full Text
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227. Cancer-associated fibroblast-targeted strategy enhances antitumor immune responses in dendritic cell-based vaccine.
- Author
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Ohshio Y, Teramoto K, Hanaoka J, Tezuka N, Itoh Y, Asai T, Daigo Y, and Ogasawara K
- Subjects
- Animals, CD8-Positive T-Lymphocytes drug effects, CD8-Positive T-Lymphocytes immunology, Cell Line, Tumor, Dendritic Cells drug effects, Female, Fibroblasts drug effects, Immunity, Cellular drug effects, Immunity, Humoral drug effects, Immunotherapy methods, Killer Cells, Natural drug effects, Killer Cells, Natural immunology, Lymph Nodes drug effects, Lymph Nodes immunology, Mice, Mice, Inbred C57BL, Mice, SCID, Neoplasms drug therapy, T-Lymphocytes, Regulatory drug effects, T-Lymphocytes, Regulatory immunology, Tumor Microenvironment drug effects, Tumor Microenvironment immunology, ortho-Aminobenzoates pharmacology, Antineoplastic Agents immunology, Cancer Vaccines immunology, Dendritic Cells immunology, Fibroblasts immunology, Immunity, Cellular immunology, Immunity, Humoral immunology, Neoplasms immunology
- Abstract
Given the close interaction between tumor cells and stromal cells in the tumor microenvironment (TME), TME-targeted strategies would be promising for developing integrated cancer immunotherapy. Cancer-associated fibroblasts (CAFs) are the dominant stromal component, playing critical roles in generation of the pro-tumorigenic TME. We focused on the immunosuppressive trait of CAFs, and systematically explored the alteration of tumor-associated immune responses by CAF-targeted therapy. C57BL/6 mice s.c. bearing syngeneic E.G7 lymphoma, LLC1 Lewis lung cancer, or B16F1 melanoma were treated with an anti-fibrotic agent, tranilast, to inhibit CAF function. The infiltration of immune suppressor cell types, including regulatory T cells and myeloid-derived suppressor cells, in the TME was effectively decreased through reduction of stromal cell-derived factor-1, prostaglandin E2 , and transforming growth factor-β. In tumor-draining lymph nodes, these immune suppressor cell types were significantly decreased, leading to activation of tumor-associated antigen-specific CD8(+) T cells. In addition, CAF-targeted therapy synergistically enhanced multiple types of systemic antitumor immune responses such as the cytotoxic CD8(+) T cell response, natural killer activity, and antitumor humoral immunity in combination with dendritic cell-based vaccines; however, the suppressive effect on tumor growth was not observed in tumor-bearing SCID mice. These data indicate that systemic antitumor immune responses by various immunologic cell types are required to bring out the efficacy of CAF-targeted therapy, and these effects are enhanced when combined with effector-stimulatory immunotherapy such as dendritic cell-based vaccines. Our mouse model provides a novel rationale with TME-targeted strategy for the development of cell-based cancer immunotherapy., (© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.)
- Published
- 2015
- Full Text
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228. Mucinous bronchioloalveolar carcinoma with K-ras mutation arising in type 1 congenital cystic adenomatoid malformation: a case report with review of the literature.
- Author
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Ishida M, Igarashi T, Teramoto K, Hanaoka J, Iwai M, Yoshida K, Kagotani A, Tezuka N, and Okabe H
- Subjects
- Adenocarcinoma, Bronchiolo-Alveolar pathology, Adenocarcinoma, Mucinous pathology, Cell Transformation, Neoplastic pathology, Child, Codon, Cystic Adenomatoid Malformation of Lung, Congenital pathology, DNA Mutational Analysis, Female, Genetic Predisposition to Disease, Humans, Lung Neoplasms pathology, Phenotype, Proto-Oncogene Proteins p21(ras), Tomography, X-Ray Computed, Adenocarcinoma, Bronchiolo-Alveolar genetics, Adenocarcinoma, Mucinous genetics, Cell Transformation, Neoplastic genetics, Cystic Adenomatoid Malformation of Lung, Congenital genetics, Lung Neoplasms genetics, Mutation, Proto-Oncogene Proteins genetics, ras Proteins genetics
- Abstract
Congenital cystic adenomatoid malformation (CCAM) of the lung is a rare hamartomatous cystic lesion, characterized by the presence of large cysts, which are histopathologically lined by pseudostratified ciliated cells. It has been recognized that rare cases of type 1 CCAM show malignant transformation, usually bronchioloalveolar carcinoma (BAC) or adenocarcinoma. Herein, we describe a case of BAC arising in type 1 CCAM with K-ras mutation. A 9-year-old Japanese girl presented with fever. Computed tomography demonstrated large cystic lesions in her right lower lung. Histopathological study of the resected specimen revealed multiple cysts, which were lined by pseudostratified ciliated cells, and occasionally interspersed with mucous cells without atypia. A small focus of proliferation of columnar cells showing lepidic growth pattern was present. These columnar cells had abundant mucin in the cytoplasm and mildly to moderately enlarged nuclei. Accordingly, a diagnosis of BAC arising in type 1 CCAM was made. Polymerase chain reaction analysis revealed K-ras mutation at codon 12 in the BAC component. The presence of mucous cell/goblet cell hyperplasia and atypical adenomatous hyperplasia is a well known phenomenon in type 1 CCAM. A recent study clearly demonstrated K-ras mutation in these lesions, which are thought to be precursors of BAC. Therefore, the concept of malignant transformation in the sequence from type 1 CCAM to mucous cell hyperplasia to atypical adenomatous hyperplasia to BAC and invasive adenocarcinoma due to K-ras mutation has been proposed. Careful histopathological analysis is important for evaluation of malignant lesions in type 1 CCAM.
- Published
- 2013
229. Thymic papillo-tubular adenocarcinoma containing a cyst: report of a case.
- Author
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Teramoto K, Kawaguchi Y, Hori T, Ishida M, Hashimoto M, Kitamura S, Motoishi M, Hanaoka J, Tezuka N, and Okabe H
- Subjects
- Adenocarcinoma complications, Humans, Male, Mediastinal Cyst complications, Middle Aged, Thymus Neoplasms complications, Adenocarcinoma diagnosis, Mediastinal Cyst diagnosis, Thymus Neoplasms diagnosis
- Abstract
We report a case of thymic papillo-tubular adenocarcinoma in a 55-year-old man, who had no symptoms. Sternotomy revealed a tumor in the anterior mediastinum, tightly adhered to the pericardium. It was resected completely. Interestingly, the tumor contained a unilocular cyst filled with mucinous fluid, suggesting that it originated from a pre-existing thymic cyst. Pathological examination of the tumor revealed a primary thymic papillo-tubular adenocarcinoma resembling a tumor of gut origin. Thymic adenocarcinomas, particularly of the tubular subtype, are extremely rare.
- Published
- 2012
- Full Text
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230. Preparation and photophysical and photoelectrochemical properties of a covalently fixed porphyrin-chemically converted graphene composite.
- Author
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Umeyama T, Mihara J, Tezuka N, Matano Y, Stranius K, Chukharev V, Tkachenko NV, Lemmetyinen H, Noda K, Matsushige K, Shishido T, Liu Z, Hirose-Takai K, Suenaga K, and Imahori H
- Abstract
Chemically converted graphene (CCG) covalently linked with porphyrins has been prepared by a Suzuki coupling reaction between iodophenyl-functionalized CCG and porphyrin boronic ester. The covalently linked CCG-porphyrin composite was designed to possess a short, rigid phenylene spacer between the porphyrin and the CCG. The composite material formed stable dispersions in DMF and the structure was characterized by spectroscopic, thermal, and microscopic measurements. In steady-state photoluminescence spectra, the emission from the porphyrin linked to the CCG was quenched strongly relative to that of the porphyrin reference. Fluorescence lifetime and femtosecond transient absorption measurements of the porphyrin-linked CCG revealed a short-lived porphyrin singlet excited state (38 ps) without yielding the porphyrin radical cation, thereby substantiating the occurrence of energy transfer from the porphyrin excited state to the CCG and subsequent rapid decay of the CCG excited state to the ground state. Consistently, the photocurrent action spectrum of a photoelectrochemical device with a SnO(2) electrode coated with the porphyrin-linked CCG exhibited no photocurrent response from the porphyrin absorption. The results obtained here provide deep insight into the interaction between graphenes and π-conjugated systems in the excited and ground states., (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2012
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231. Carbon nanotube wiring of donor-acceptor nanograins by self-assembly and efficient charge transport.
- Author
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Umeyama T, Tezuka N, Kawashima F, Seki S, Matano Y, Nakao Y, Shishido T, Nishi M, Hirao K, Lehtivuori H, Tkachenko NV, Lemmetyinen H, and Imahori H
- Subjects
- Models, Molecular, Molecular Structure, Particle Size, Porphyrins chemistry, Surface Properties, Nanotubes, Carbon chemistry
- Published
- 2011
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232. Formation of single-walled carbon nanotube thin films enriched with semiconducting nanotubes and their application in photoelectrochemical devices.
- Author
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Wei L, Tezuka N, Umeyama T, Imahori H, and Chen Y
- Subjects
- Equipment Design, Equipment Failure Analysis, Conductometry instrumentation, Membranes, Artificial, Nanotechnology instrumentation, Nanotubes, Carbon chemistry, Nanotubes, Carbon ultrastructure, Photometry instrumentation, Semiconductors
- Abstract
Single-walled carbon nanotube (SWCNT) thin films, containing a high-density of semiconducting nanotubes, were obtained by a gel-centrifugation method. The agarose gel concentration and centrifugation force were optimized to achieve high semiconducting and metallic nanotube separation efficiency at 0.1 wt% agarose gel and 18,000g. The thickness of SWCNT films can be precisely controlled from 65 to 260 nm with adjustable transparency. These SWCNT films were applied in photoelectrochemical devices. Photocurrents generated by semiconducting SWCNT enriched films are 15-35% higher than those by unsorted SWCNT films. This is because of reducing exciton recombination channels as a result of the removal of metallic nanotubes. Thinner films generate higher photocurrents because charge carriers have less chances going in metallic nanotubes for recombination, before they can reach electrodes. Developing more scalable and selective methods for high purity semiconducting SWCNTs is important to further improve the photocurrent generation efficiency by using SWCNT-based photoelectrochemical devices.
- Published
- 2011
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233. [Liver dysfunction during treatment of latent tuberculosis infection].
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Nakazono T, Tezuka N, Tagawa H, Takayanagi K, Sugita H, Takase A, Yamaguchi T, and Shimao T
- Subjects
- Adolescent, Adult, Female, Humans, Male, Middle Aged, Chemical and Drug Induced Liver Injury etiology, Isoniazid adverse effects, Latent Tuberculosis drug therapy
- Abstract
Purpose: The indications for treatment for latent tuberculosis infection were revised in 2007 to reflect that any subject with a higher risk of tuberculosis regardless of age should be treated. We worried about the incidence of liver dysfunction due to isoniazid (INH) in patients older than 30 yrs. of age. We evaluated the frequency of liver dysfunction due to INH according to age and discussed the possibility of its prevention., Methods: We reviewed the clinical records of 99 patients younger than 29 yrs. and 229 patients older than 30 yrs. who were treated for latent tuberculosis infection from August 2007 to December 2008 at our clinic. The liver function tests (AST and ALT) were performed before the treatment, one and a half months after the start of the treatment, and almost every month during the treatment. We defined liver dysfunction as an AST and/or ALT greater than 100 IU/L., Results: Seven out of the 99 younger patients (7.1%) and 42 out of the 229 (18.3%) older patients developed liver dysfunction. The difference between the two age groups was statistically significant according to the chi-square test (p < 0.01). After the occurrence of liver dysfunction, 35 out of 49 patients (71%) completed the treatment by maintaining the same or a decreased dose of INH, while the medication was discontinued in 9 patients who were then followed up by chest X-ray examination. Two of these 49 patients discontinued the medication by themselves., Conclusions: The frequency of liver damage due to INH was higher in persons older than 30 yrs. In this group, 3 persons developed severe liver damage with ALT and/or AST higher than 1000 IU/L. Early detection is required to avoid serious damage. Thus, we decided to perform liver function tests more often, i.e., at 2 weeks after the onset of treatment and every month thereafter.
- Published
- 2011
234. Good solvent effects of C70 cluster formations and their electron-transporting and photoelectrochemical properties.
- Author
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Tezuka N, Umeyama T, Matano Y, Shishido T, Kawasaki M, Nishi M, Hirao K, Lehtivuori H, Tkachenko NV, Lemmetyinen H, Honsho Y, Seki S, and Imahori H
- Subjects
- Absorption, Acetonitriles chemistry, Electrochemistry, Electron Transport, Electrophoresis, Microwaves, Spectrophotometry, Ultraviolet, Surface Properties, Carbon chemistry, Photochemical Processes, Solvents chemistry
- Abstract
Good solvent effects of C(70) cluster formations and their electron-transporting and photoelectrochemical properties have been systematically examined for the first time. Nano-to-micrometer scale assemblies of C(70) with different morphologies were prepared by rapidly injecting poor solvent (i.e., acetonitrile) into a solution of C(70) dissolved in various good solvents (i.e., benzene, toluene, chlorobenzene, etc). The cluster morphology engineering was successfully achieved by changing the good solvent, yielding the spherical, rodlike, or platelike clusters in the mixed solvents. The clusters of C(70) were electrophoretically deposited onto a nanostructured SnO(2) electrode to examine the photoelectrochemical properties under the white light or monochromatic light illumination. The maximum incident photon-to-current efficiency (IPCE) varied from 0.8 to 10% depending on the combinations of the poor-good solvents. The differences in the IPCE values are discussed in terms of the surface area, thickness, and electron mobility of the deposited cluster films. The electron mobility is found to be the most predominant factor for the IPCE, indicating the importance of the electron-transporting process in the overall photocurrent generation. In addition, the electron mobility is closely correlated with the underlying molecular alignment and the resultant cluster structure. Thus, these results will provide basic clue for the design of C(70)-based molecular devices including the organic photovoltaics.
- Published
- 2010
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235. Dispersion of carbon nanotubes by photo- and thermal-responsive polymers containing azobenzene unit in the backbone.
- Author
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Umeyama T, Kawabata K, Tezuka N, Matano Y, Miyato Y, Matsushige K, Tsujimoto M, Isoda S, Takano M, and Imahori H
- Subjects
- Isomerism, Solvents chemistry, Spectroscopy, Near-Infrared, Temperature, Azo Compounds chemistry, Nanotubes, Carbon chemistry, Polymers chemistry
- Abstract
Photo- and thermal-responsive polymers containing azobenzene units in the main chain have been utilized as removable dispersants for single-walled carbon nanotubes (SWNTs) in organic solvents. Intermolecular interactions between SWNTs and the polymers are reversibly controllable by tuning the trans-cis composition.
- Published
- 2010
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236. Differences in left ventricular dyssynchrony between high septal pacing and apical pacing in patients with normal left ventricular systolic function.
- Author
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Yoshikawa H, Suzuki M, Tezuka N, Otsuka T, and Sugi K
- Subjects
- Echocardiography, Female, Humans, Male, Middle Aged, Ventricular Dysfunction, Left diagnostic imaging, Cardiac Pacing, Artificial methods, Ventricular Dysfunction, Left physiopathology, Ventricular Function, Left physiology
- Abstract
Background and Purpose: Permanent right ventricular apical pacing can result in heart failure due to ventricular mechanical dyssynchrony. The purpose of the study was to define differences in left ventricular dyssynchrony between high septal pacing and apical pacing using tissue Doppler imaging (TDI) and two-dimensional (2D) speckle-tracking echocardiography (STE)., Methods: The subjects were 60 patients with normal left ventricular systolic function who underwent implantation of a permanent pacemaker. Patients were divided into two groups with high septal pacing (n=36) and conventional right apical pacing (n=24). Left ventricular dyssynchrony was measured using TDI and 2D-STE. The time difference (TD) between the earliest and latest activated segments obtained from each systolic velocity curve by TDI was defined as TD-TDI. The time differences obtained from systolic strain curves obtained by 2D-STE were defined as TD-RS for radial strain, TD-CS for circumferential strain, and TD-LS for longitudinal strain., Results: The high septal pacing group had significantly shorter TD-TDI (20.0+/-24.3ms vs. 59.7+/-43.0ms, p<0.0001), TD-RS (13.5+/-19.9ms vs. 45.8+/-24.6ms, p<0.0001), and TD-LS (42.7+/-22.0ms vs. 66.6+/-26.8ms, p=0.001) values compared to the apical pacing group. There was no significant difference in TD-CS between the two groups., Conclusion: Left ventricular dyssynchrony was smaller in patients with high septal pacing. The results show that 2D-STE is useful for detection of differences in left ventricular mechanical dyssynchrony in patients with permanent pacemaker implantation, in addition to TDI., (Copyright 2010 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2010
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237. Selective formation and efficient photocurrent generation of [70]fullerene-single-walled carbon nanotube composites.
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Umeyama T, Tezuka N, Seki S, Matano Y, Nishi M, Hirao K, Lehtivuori H, Tkachenko NV, Lemmetyinen H, Nakao Y, Sakaki S, and Imahori H
- Subjects
- Chlorobenzenes chemistry, Nanotubes, Carbon ultrastructure, Photochemical Processes, Solar Energy, Spectrophotometry, Ultraviolet, Tin Compounds chemistry, Fullerenes chemistry, Nanotubes, Carbon chemistry
- Published
- 2010
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238. Inhibition of transforming growth factor-beta-mediated immunosuppression in tumor-draining lymph nodes augments antitumor responses by various immunologic cell types.
- Author
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Fujita T, Teramoto K, Ozaki Y, Hanaoka J, Tezuka N, Itoh Y, Asai T, Fujino S, Kontani K, and Ogasawara K
- Subjects
- Animals, Antigens, Neoplasm immunology, DNA, Female, Mice, Mice, Inbred C57BL, Transforming Growth Factor beta genetics, Transforming Growth Factor beta physiology, Immunosuppression Therapy, Lymph Nodes immunology, Transforming Growth Factor beta antagonists & inhibitors
- Abstract
Tumor-draining lymph nodes (DLN) are the most important priming sites for generation of antitumor immune responses. They are also the location where an immunosuppressive cytokine, transforming growth factor-beta (TGF-beta), plays a critical role in suppressing these antitumor immune responses. We focused on TGF-beta-mediated immunosuppression in DLNs and examined whether local inhibition of TGF-beta augmented antitumor immune responses systemically in tumor-bearing mice models. For inhibition of TGF-beta-mediated immunosuppression in DLNs, C57BL/6 mice subcutaneously bearing E.G7 tumors were administered plasmid DNA encoding the extracellular domain of TGF-beta type II receptor fused to the human IgG heavy chain (TGFR DNA) i.m. near the established tumor. In DLNs, inhibition of TGF-beta suppressed the proliferation of regulatory T cells and increased the number of tumor antigen-specific CD4(+) or CD8(+) cells producing IFN-gamma. Enhancement of antitumor immune responses in DLNs were associated with augmented tumor antigen-specific cytotoxic and natural killer activity in spleen as well as elevated levels of tumor-specific antibody in sera. The growth of the established metastatic as well as primary tumors was effectively suppressed via augmented antitumor immune responses. Inhibition of TGF-beta-mediated immunosuppression in DLNs is significantly associated with augmented antitumor responses by various immunocompetent cell types. This animal model provides a novel rationale for molecular cancer therapeutics targeting TGF-beta.
- Published
- 2009
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239. Ultra-low-dose computed tomography system with a flat panel detector: assessment of radiation dose reduction and spatial and low contrast resolution.
- Author
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Nagatani Y, Nitta N, Takahashi M, Tezuka N, Nakano Y, Ikeda M, Kirino Y, Hashimoto K, Otani H, Murakami Y, and Murata K
- Subjects
- Aged, Algorithms, Data Display, Equipment Design, Female, Humans, Image Processing, Computer-Assisted methods, Lung blood supply, Lung diagnostic imaging, Male, Middle Aged, Observer Variation, Phantoms, Imaging, Posture, Radiographic Image Interpretation, Computer-Assisted methods, Sensitivity and Specificity, Tomography, X-Ray Computed methods, X-Ray Intensifying Screens, Cone-Beam Computed Tomography instrumentation, Cone-Beam Computed Tomography methods, Radiation Dosage, Radiographic Image Enhancement instrumentation
- Abstract
Purpose: The aim of this study was to introduce a prototype cone-beam computed tomography system equipped with a flat panel detector (FPD-CT system) and measure its radiation dose and spatial and lowcontrast resolution., Materials and Methods: A patient was rotated in a sitting position, and cone beam data were acquired with the flat panel detector from a fixed X-ray tube. Absorbed dose, spatial and low-contrast resolution, and variation in the CT attenuation value were assessed quantitatively in the acrylic phantom. The visibility of normal blood vessels in clinical images of seven patients was analyzed qualitatively by five board-certified radiologists. These quantitative and qualitative data were compared between the FPD-CT system and multidetector row CT (MDCT)., Results: Minimal low-contrast sensitivity and a moderate spatial resolution were demonstrated in images of central lung fields acquired by FPD-CT. The absorbed dose in the FPD-CT system decreased to approximately 2.5% of the dose in the MDCT system., Conclusion: Considering crossover structures in normal blood vessels and bronchi in the central areas of lung fields, this result implies that fairly acceptable spatial resolution can be realized with FPD-CT for detection and frequent follow-up of pulmonary abnormalities in the central lung fields.
- Published
- 2008
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240. Highly spin-polarized materials and devices for spintronics ∗ .
- Author
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Inomata K, Ikeda N, Tezuka N, Goto R, Sugimoto S, Wojcik M, and Jedryka E
- Abstract
The performance of spintronics depends on the spin polarization of the current. In this study half-metallic Co-based full-Heusler alloys and a spin filtering device (SFD) using a ferromagnetic barrier have been investigated as highly spin-polarized current sources. The multilayers were prepared by magnetron sputtering in an ultrahigh vacuum and microfabricated using photolithography and Ar ion etching. We investigated two systems of Co-based full-Heusler alloys, Co
2 Cr1 - x Fex Al (CCFA( x )) and Co2 FeSi1 - x Alx (CFSA( x )) and revealed the structure and magnetic and transport properties. We demonstrated giant tunnel magnetoresistance (TMR) of up to 220% at room temperature and 390% at 5 K for the magnetic tunnel junctions (MTJs) using Co2 FeSi0.5 Al0.5 (CFSA(0.5)) Heusler alloy electrodes. The 390% TMR corresponds to 0.81 spin polarization for CFSA(0.5) at 5 K. We also investigated the crystalline structure and local structure around Co atoms by x-ray diffraction (XRD) and nuclear magnetic resonance (NMR) analyses, respectively, for CFSA films sputtered on a Cr-buffered MgO (001) substrate followed by post-annealing at various temperatures in an ultrahigh vacuum. The disordered structures in CFSA films were clarified by NMR measurements and the relationship between TMR and the disordered structure was discussed. We clarified that the TMR of the MTJs with CFSA(0.5) electrodes depends on the structure, and is significantly higher for L21 than B2 in the crystalline structure. The second part of this paper is devoted to a SFD using a ferromagnetic barrier. The Co ferrite is investigated as a ferromagnetic barrier because of its high Curie temperature and high resistivity. We demonstrate the strong spin filtering effect through an ultrathin insulating ferrimagnetic Co-ferrite barrier at a low temperature. The barrier was prepared by the surface plasma oxidization of a CoFe2 film deposited on a MgO (001) single crystal substrate, wherein the spinel structure of CoFe2 O4 (CFO) and an epitaxial relationship of MgO(001)[100]/CoFe2 (001)]110]/CFO(001)[100] were induced. A SFD consisting of CoFe2 /CFO/Ta on a MgO (001) substrate exhibits the inverse TMR of - 124% at 10 K when the configuration of the magnetizations of CFO and CoFe2 changes from parallel to antiparallel. The inverse TMR suggests the negative spin polarization of CFO, which is consistent with the band structure of CFO obtained by first principle calculation. The - 124% TMR corresponds to the spin filtering efficiency of 77% by the CFO barrier.- Published
- 2008
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241. HER1-4 expression status correlates with the efficacy of gefitinib treatment and tumor cell proliferative activity in non-small cell lung cancer.
- Author
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Fujita M, Ishida M, Tezuka N, Fujino S, Asai T, and Okabe H
- Abstract
The expression of human epidermal growth factor receptor (HER) family proteins was examined in specimens of 52 non-small cell lung cancers to evaluate their significance in tumor cell proliferation and their response to gefitinib. Epidermal growth factor receptor (EGFR; HER1) protein was expressed most frequently (43/52 cases). HER2 and HER3 were detected in 13 and 18 cases, respectively. HER4 was detected in only one case of squamous cell carcinoma. HER3 was expressed preferentially in adenocarcinomas of the papillary type. In most HER3-positive cases with or without other HER family proteins, the Ki-67 labelling index (18.2%) was significantly lower than in HER3-negative cases (30.6%) (P<0.05). Adenocarcinomas co-expressing EGFR, HER2 and HER3 frequently showed both a good response to gefitinib (6/7 cases) and a mutation of EGFR (4/6 cases). However, cases with a favourable response to gefitinib did not always express EGFR. Gefitinib appears to suppress growth signal pathways other than EGFR-tyrosine kinase activation in non-small cell lung cancers.
- Published
- 2008
242. Enhanced tunnel magnetoresistance due to spin dependent quantum well resonance in specific symmetry states of an ultrathin ferromagnetic electrode.
- Author
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Niizeki T, Tezuka N, and Inomata K
- Abstract
Spin dependent quantum well resonance has been investigated in fully epitaxial magnetic tunnel junctions with Fe(001)/MgO(001)/ultrathin Fe(001)/Cr(001) structure. The dI/dV spectra clearly show the resonant peaks which shift systematically depending on the thickness of an ultrathin electrode as predicted in ab initio calculation [Zhong-Yi Lu et al, Phys. Rev. Lett. 94, 207210 (2005)]. The magnetotransport is strongly modulated at the same bias voltage as the resonant peaks. This control of the magnetotransport in magnetic tunnel junctions at a specific bias voltage will contribute to the development of active spintronic devices.
- Published
- 2008
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243. Pulmonary infarction associated with bronchogenic carcinoma.
- Author
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Takahashi M, Murakami Y, Nitta N, Murata K, Tezuka N, Fujino S, and Okabe H
- Subjects
- Adenocarcinoma complications, Adenocarcinoma therapy, Carcinoma, Bronchogenic complications, Carcinoma, Bronchogenic therapy, Carcinoma, Small Cell complications, Carcinoma, Small Cell therapy, Contrast Media administration & dosage, Diagnosis, Differential, Humans, Incidental Findings, Lung diagnostic imaging, Lung surgery, Lung Neoplasms complications, Lung Neoplasms therapy, Male, Middle Aged, Pulmonary Infarction etiology, Pulmonary Infarction surgery, Radiographic Image Enhancement methods, Tomography, X-Ray Computed, Adenocarcinoma diagnosis, Carcinoma, Bronchogenic diagnosis, Carcinoma, Small Cell diagnosis, Lung Neoplasms diagnosis, Pulmonary Infarction diagnosis
- Abstract
Computed tomography findings of pathologically proven pulmonary infarction associated with bronchogenic carcinoma are reported for two patients. In one case, the infarction was demonstrated as a well-defined pleura-based large nodule in the peripheral portion of the same lobe of the tumor. The nodule had a smooth, convex border and a linear strand from the apex of the lesion toward the hilum. The obstruction of the subsegmental pulmonary artery due to tumor invasion was considered the cause of pulmonary infarction. In the second case, the infarction was demonstrated as a rapidly appeared, pleura-based consolidation in the same lobe of the tumor with a blurred border. Obstruction of the pulmonary vein by a tumor might have played an important role in the development of the pulmonary infarction in association with a large pulmonary artery obstruction. We conclude that pulmonary infarction should be considered as a differential diagnosis when peripheral pulmonary nodules or masses are located in the same lobe as the primary cancer.
- Published
- 2008
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244. Clusterization, electrophoretic deposition, and photoelectrochemical properties of fullerene-functionalized carbon nanotube composites.
- Author
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Umeyama T, Tezuka N, Fujita M, Hayashi S, Kadota N, Matano Y, and Imahori H
- Abstract
We have successfully developed a new methodology for the self-organization of C(60) molecules on the sidewall of carbon nanotubes for use in photoelectrochemical devices. Novel nanocarbon composites of fullerene (e.g., C(60)) and highly soluble, chemically functionalized single-walled carbon nanotubes (f-SWNT) have been prepared by the rapid injection of a poor solvent (e.g., acetonitrile) into a mixed solution of C(60) and f-SWNT in o-dichlorobenzene. Measurements by using scanning electron microscopy of cast samples revealed that the composites are categorized into three groups; i) f-SWNT bundles covered with layers of C(60) molecules, ii) round, large C(60) clusters (sizes of 500-1000 nm) containing f-SWNT, and iii) typical, round C(60) clusters (sizes of 150-250 nm). The electrophoretic deposition of the composites onto a nanostructured SnO(2) electrode yielded the hierarchical film with a gradient composition depending on the difference in the mobilities of C(60) and f-SWNT during the electrophoretic process. The composite film exhibited an incident photon-to-photocurrent efficiency as high as 18 % at lambda=400 nm under an applied potential of 0.05 V vs. SCE. The photocurrent generation efficiency is the highest value among carbon nanotube-based photoelectrochemical devices in which carbon nanotubes are deposited electrophoretically, electrostatically or covalently onto semiconducting electrodes. The highly aligned structure of C(60) molecules on f-SWNT can rationalize the efficient photocurrent generation. The results obtained here will provide valuable information on the design of carbon nanotube-based molecular devices.
- Published
- 2008
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245. Good syndrome coexisting with leukopenia.
- Author
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Ohuchi M, Inoue S, Hanaoka J, Igarashi T, Tezuka N, Ozaki Y, and Teramoto K
- Subjects
- Humans, Male, Middle Aged, Syndrome, Thymectomy, Thymoma immunology, Thymoma surgery, Thymus Neoplasms immunology, Thymus Neoplasms surgery, Agammaglobulinemia complications, Immunologic Deficiency Syndromes complications, Leukopenia complications, Thymoma complications, Thymus Neoplasms complications
- Abstract
A 61-year-old man was admitted to our hospital for further examinations of a mediastinal mass. He had underwent an extended thymothymectomy, and had a tumor that was diagnosed as a type B1 thymoma, according to the World Health Organization. One year after surgery he was admitted again for recurrent diarrhea and pneumonia. Laboratory data revealed severe hypogammaglobulinemia with leukopenia. He was diagnosed with Good syndrome with leukopenia. Regular gamma globulin and figrastim injections were successful in keeping the patient symptom free. The prognosis of patients with Good syndrome and leukopenia is very poor; therefore, immediate diagnosis is important. The development of infectious diseases in a patient with thymoma or after the resection of thymoma mandates early and comprehensive immunologic investigation.
- Published
- 2007
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246. GRB10 binds to LRP6, the Wnt co-receptor and inhibits canonical Wnt signaling pathway.
- Author
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Tezuka N, Brown AM, and Yanagawa S
- Subjects
- Animals, Axin Protein, Binding Sites, Humans, Low Density Lipoprotein Receptor-Related Protein-6, Mice, RNA Interference, Rats, Repressor Proteins antagonists & inhibitors, Signal Transduction drug effects, beta Catenin antagonists & inhibitors, src Homology Domains physiology, GRB10 Adaptor Protein metabolism, Receptors, LDL metabolism, Wnt Proteins physiology
- Abstract
Low-density lipoprotein receptor-related protein 6 (LRP6) is a component of cell-surface receptors for Wnt proteins and Wnt is known to promote recruitment of Axin by LRP6 thereby inhibiting beta-catenin's degradation. We show here that growth factor receptor-bound protein10 (GRB10), a multi-modular adaptor protein that is known to associate with several transmembrane tyrosine kinase receptors, binds to the intracellular portion of LRP6 and negatively regulates Wnt signaling. GRB10 overexpression suppressed Wnt3a-, and LRP6-induced but not beta-catenin-induced TCF-dependent-reporter activities in HEK293T cells, suggesting that GRB10 functions upstream of beta-catenin. Actually, GRB10 overexpression attenuated the Wnt3a-induced accumulation of beta-catenin. In addition, RNAi-mediated down-regulation of endogenous GRB10 stimulated Wnt3a-induced reporter activities, indicating that GRB10 is indeed a novel negative regulator of the Wnt signaling pathway. The finding that GRB10 interferes with the binding of Axin to LRP6 indicated a possible molecular mechanism by which the overexpression of GRB10 suppresses Wnt signaling.
- Published
- 2007
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247. Assessment of diaphragmatic motion after lung resection using magnetic resonance imaging.
- Author
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Takazakura R, Takahashi M, Nitta N, Sawai S, Tezuka N, Fujino S, and Murata K
- Subjects
- Adult, Aged, Analysis of Variance, Female, Follow-Up Studies, Humans, Image Processing, Computer-Assisted methods, Male, Middle Aged, Postoperative Period, Posture, Respiration, Supine Position, Time Factors, Diaphragm physiopathology, Lung surgery, Lung Neoplasms surgery, Magnetic Resonance Imaging methods, Motion
- Abstract
Purpose: The aim of this study was to assess quantitatively the impairment of diaphragmatic motion after lung resection, with special reference to the location of the resected lobe, duration of the postoperative period, and patient posture. We used magnetic resonance imaging to make the assessments., Materials and Methods: In 44 patients (29 men, 15 women; mean age 62.2 years) with lung cancer, diaphragmatic motion was measured during maximum deep, slow breathing using a spoiled gradient-recalled echo sequence before and after lung resection. The study group consisted of 34 patients who were examined using a 1.5-T unit in the supine position and 10 patients using a vertically open 0.5-T unit in both the sitting and supine positions. The influence of surgery site and patient posture on diaphragmatic motion after lung resection was investigated., Results: In all cases after lung resection, diaphragmatic motion on the operated side was significantly decreased (P < 0.001), and that on the nonoperated side was significantly increased (P = 0.045). After left upper lobectomy and right bilobectomy, the diaphragmatic motion on the operated side was significantly decreased (P < 0.001), and that of the other side was significantly increased (P < 0.001). The diaphragmatic motion was not significantly changed after right middle lobectomy. The diaphragmatic motion on the operated side was impaired significantly more (P = 0.035) in the supine position than in the sitting position., Conclusion: After lobe resection, diaphragmatic motion was impaired more significantly in the supine than in the sitting position; and it differed according to the location of the resected lobe. The improvement in diaphragmatic function after lobectomy was observed over a period of 3-24 months.
- Published
- 2007
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248. Preparation of fully activated dendritic cells capable of priming tumor-specific cytotoxic T lymphocytes in patients with metastatic cancer using penicillin-killed streptococcus pyogenes (OK432) and anti-CD40 antibody.
- Author
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Kontani K, Teramoto K, Ozaki Y, Sawai S, Tezuka N, Ishida H, Kajino K, Fujino S, Yamauchi A, Taguchi O, Kannagi R, Yokomise H, and Ogasawara K
- Subjects
- Aged, Antibodies pharmacology, Antigen Presentation, Antigen-Presenting Cells chemistry, Antigen-Presenting Cells transplantation, Antigens, CD analysis, CD40 Antigens immunology, Cytokines metabolism, Cytotoxicity, Immunologic, Dendritic Cells chemistry, Dendritic Cells transplantation, Female, Humans, Interferon-gamma metabolism, Lung Neoplasms pathology, Male, Middle Aged, Neoplasm Metastasis, Penicillins pharmacology, Streptococcus pyogenes drug effects, Antigen-Presenting Cells immunology, Dendritic Cells immunology, Immunotherapy, Adoptive methods, Lung Neoplasms therapy, Lymphocytes, Tumor-Infiltrating immunology, Streptococcus pyogenes immunology, T-Lymphocytes, Cytotoxic immunology
- Abstract
In order to achieve sufficient therapeutic potency, it has been proposed that vaccine therapy with dendritic cells needs to be combined with manipulation of immunological checkpoints, such as inhibition of regulatory T cells and blockade of negative signals, and enhancement of T cell trafficking to tumor sites. In the combinatorial cancer immunotherapy, use of matured/activated dendritic cells (DCs) with more potent antigen presenting capacity seems to be essential for eliciting anti-tumor immune responses. We herein established an ex vivo induction strategy for activated DCs capable of eliciting efficient tumor antigen-specific cytotoxic T lymphocytes (CTLs) from patients with metastatic cancer as well as healthy donors. Immature DCs were matured by 48-h culture in the presence of anti-CD40 antibody and penicillin-killed streptococcus pyogenes (OK432). Supplementation with both anti-CD40 and OK432 resulted in induction of activated DCs with higher surface expression of CD80, CD83, CD86 and major histocompatibility complex class II antigens, compared with other mature DCs that were induced by the combination of anti-CD40 with tumor necrosis factor-alpha or lipopolysaccharide. In analysis of the produced cytokine profiles, the activated DCs produced the highest T-helper 1-type cytokines for at least 72 h. Furthermore, the activated DCs, pulsed with tumor-associated antigen peptide, elicited in vitro tumor-specific CTLs, but DCs activated with other combinations did not in cancer patients. Therefore, we suggest that the activated DCs studied here might be used as a basic element for the combinatorial cancer immunotherapy.
- Published
- 2007
249. Successful tumor eradication was achieved by collaboration of augmented cytotoxic activity and anti-angiogenic effects following therapeutic vaccines containing helper-activating analog-loaded dendritic cells and tumor antigen DNA.
- Author
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Teramoto K, Kontani K, Fujita T, Ozaki Y, Sawai S, Tezuka N, Fujino S, Itoh Y, Taguchi O, Kannagi R, and Ogasawara K
- Subjects
- Animals, Cancer Vaccines administration & dosage, Cancer Vaccines immunology, Cell Line, Tumor, Cell Proliferation drug effects, Columbidae, Cytochromes c immunology, Dendritic Cells drug effects, Dendritic Cells immunology, Disease Models, Animal, Epitopes immunology, Female, Flow Cytometry, Immunohistochemistry, Interferon-gamma analysis, Lymphocytes immunology, Mice, Mice, Inbred C57BL, Neoplasms pathology, Ovalbumin immunology, Peptide Fragments administration & dosage, Peptide Fragments immunology, Spleen immunology, Antigens, Neoplasm immunology, Cancer Vaccines therapeutic use, DNA, Neoplasm immunology, Dendritic Cells transplantation, Neoplasms immunology, Neoplasms therapy, Peptide Fragments therapeutic use
- Abstract
We reported previously that pigeon cytochrome c-derived peptides (Pan-IA), which bind broad ranges of MHC class II molecules efficiently, activate T helper (Th) function in mice. In an experimental model, Pan-IA DNA vaccines augmented antitumor immunity in tumor antigen-immunized mice. To elicit more potent antitumor immunity and to eradicate tumors in a therapeutic setting, Pan-IA-loaded dendritic cells (DCs) were inoculated in combination with vaccines including ovalbumin (OVA) antigen DNA in tumor-bearing mice. Seventy percent of the immunized mice survived tumor-free for at least 4 months after treatment. In contrast, mice vaccinated with OVA DNA, either with or without naïve DCs, did not eliminate the tumors and died within 5 weeks. Only in mice vaccinated with OVA DNA and Pan-IA-loaded DCs were both cytotoxic and helper responses specific for OVA induced at the spleen and tumor sites as well as at the vaccination sites. Furthermore, accumulation of OVA-specific CD4(+) and CD8(+) T lymphocytes and interferon-gamma-mediated anti-angiogenesis were observed in the tumors of these mice. Thus, the combined vaccination primed both tumor-specific cytotoxicity and helper immunity resulting in augmented tumor lysis ability and anti-angiogenic effects. This is the first report to show that most established tumors were successfully eradicated by collaboration of potent antitumor immunity and anti-angiogenic effects by vaccination with tumor antigens and helper-activating analogs. This novel vaccination strategy is broadly applicable, regardless of identifying helper epitopes in target molecules, and contributes to the development of therapeutic cancer vaccines.
- Published
- 2007
- Full Text
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250. Quantum oscillation of the tunneling conductance in fully epitaxial double barrier magnetic tunnel junctions.
- Author
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Nozaki T, Tezuka N, and Inomata K
- Abstract
We investigated spin-dependent tunneling conductance properties in fully epitaxial double MgO barrier magnetic tunnel junctions with layered nanoscale Fe islands as a middle layer. Clear oscillations of the tunneling conductance were observed as a function of the bias voltage. The oscillation, which depends on the middle layer thickness and the magnetization configuration, is interpreted by the modulation of tunneling conductance due to the spin-polarized quantum well states created in the middle Fe layer. This first observation of the quantum size effect in the fully epitaxial double barrier magnetic tunnel junction indicates great potential for the development of the spin-dependent resonant tunneling effect in coherent tunneling regime.
- Published
- 2006
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