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968 results on '"Wang, Yiyan"'

216. Recoding Rare Codons to Unnatural Amino Acids in Bacteria

Author

Wang, Yiyan

Subjects
Molecular biology, Biochemistry, Chemistry, aminoacyl-tRNA synthetase, protein engineering, protein evolution, sense codon, translation, unnatural amino acid
Abstract

The ability to introduce unnatural amino acids (UAAs) with novel chemical, physical and biological properties into proteins adds a new dimension to study the structure and function of proteins. In order to site-specifically incorporate UAAs into proteins in vivo, one unique 'blank' codon designated for UAAs and an orthogonal aminoacyl-tRNA synthetase (aaRS) /tRNA pair are required. However there are very few 'blank' codons, which dramatically limits the efficiency and diversity of UAA incorporation. Till now, only three stop codons and few frame-shift quadruplets, such as AGGA, were reported to be used for UAAs. In order to provide more blank codons, we reassigned rare sense codons to unnatural amino acids. We created new UAA-RS/tRNA pairs from TyrRS/tRNA to recode two sense codons (AGG or AGG) from arginine to UAAs. Also, multiple UAAs were incorporated into one protein simultaneously in response to a sense codon and the amber codon UAG by using the new UAA-RS/tRNA pair and PylRS/tRNACUA. To overcome the difficulties during sense codon reassignment, we adopted the following strategies. First, a sensitive probe was created for the detection of a single amino acid mutation due to the success of codon reassignment. Second, new UAA-RS/tRNA pairs were evolved to effectively and orthogonally recode the sense codon. Third, the UAA incorporation conditions were optimized to maximize the UAA incorporation efficiency and to minimize the toxicity caused by global suppressing of the sense codon. Mass spectrometry results showed several UAAs were introduced into proteins in response to AGG or AGA at a single position by using the newly evolved UAA-RS/tRNA pair, and the arginine suppression rates were over 90%. Further, multiple UAAs were simultaneously incorporated into proteins in responding to AGG and UAG, respectively. Double UAA incorporation rates were about 80% or above. In addition, fluorescent dye tagging, and PEGylation were used to confirm the successful incorporation of target UAAs. In this work, for the first time the resource of 'blank' codons was successfully expanded to sense codons. The same strategies can be applied to reassign many other sense codons which contain 61 potential candidate codons for 20 canonical amino acids.

Published
2013

218. Equalization Filter Algorithm of Music Signal Based on Time-Frequency Domain Analysis

Author

Wang Yiyan

Subjects
Computer science, Noise (signal processing), Process (computing), Equalization (audio), 020206 networking & telecommunications, 02 engineering and technology, 01 natural sciences, Signal, Transmission (telecommunications), Frequency domain, 0103 physical sciences, 0202 electrical engineering, electronic engineering, information engineering, Time domain, 010301 acoustics, Algorithm, Filter algorithm
Abstract

In order to effectively remove the noise existing in the transmission process of music signal, this paper discusses the music signal filtering algorithm from two different angles of time domain and frequency domain, and designs two equalization filters with adjustable parameters. And through the experimental simulation algorithm, the corresponding first-order and second-order low-frequency signal filtering cases are discussed. The effectiveness of the proposed method is demonstrated. At the same time, the method can be generalized to the case of high-order equalization filtering.

Published
2019
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223. Educational attainment could be a protective factor against obstructive sleep apnea: a study based on Mendelian randomization

Author

Song, Lijuan, primary, Li, Hao, additional, Wang, Jia, additional, Xie, Junyang, additional, Chen, Gui, additional, Liang, Tianhao, additional, Wang, Yiyan, additional, Ye, Liyao, additional, Wang, Xiaofen, additional, Kuang, Xiaoxuan, additional, Ren, Mei, additional, Ye, Jinfeng, additional, Tang, Yaqian, additional, Ji, Ketong, additional, Liao, Wenjing, additional, and Zhang, Xiaowen, additional

Published
2021
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228. Electronic structure and open-orbit Fermi surface topology in isostructural semimetals NbAs2 and W2As3 with extremely large magnetoresistance.

Author

Lou, Rui, Wang, Yiyan, Zhao, Lingxiao, Xu, Chenchao, Li, Man, Chen, Xiaoyang, Zhang, Anmin, Huang, Yaobo, Cao, Chao, Chen, Genfu, Xia, Tianlong, Zhang, Qingming, Ding, Hong, and Wang, Shancai

Subjects
*FERMI surfaces, *ELECTRONIC structure, *PHOTOELECTRON spectroscopy, *SEMIMETALS, *TOPOLOGY, *SPACE groups, *MAGNETORESISTANCE
Abstract

In transition-metal dipnictides TmPn2 (Tm = Ta and Nb; Pn = P, As, and Sb), the origin of extremely large magnetoresistance (XMR) is yet to be studied by the direct visualization of the experimental band structures. Here, using angle-resolved photoemission spectroscopy, we map out the three-dimensional electronic structure of NbAs2. The open-orbit topology contributes to a non-negligible part of the Fermi surfaces (FSs), like that of the isostructural compound MoAs2, where the open FS is proposed to likely explain the origin of XMR. We further demonstrate the observation of open characters in the overall FSs of W2As3, which is also a XMR semimetal with the same space group of C12/m1 as the TmPn2 family and MoAs2. Our results suggest that the open-orbit FS topology may be a shared feature between XMR materials with the space group of C12/m1 and, thus, could possibly play a role in determining the corresponding XMR effect together with the electron–hole compensation. [ABSTRACT FROM AUTHOR]

Published
2022
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243. Superconducting Interfaces between Weyl Semimetal and Normal Metal

Author

Zhu, Wenliang, primary, Wang, Yiyan, additional, Li, Jing, additional, Huang, Yifei, additional, Dong, Qingxin, additional, Hou, Xingyuan, additional, Zhao, Lingxiao, additional, Zhang, Shuai, additional, Yang, Huaixin, additional, Ren, Zhian, additional, Shan, Lei, additional, and Chen, Genfu, additional

Published
2020
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247. How do CD8+ T cells complete DNA replication in less than 4 hours?

Author

Sugitani, Norie, primary, Moiseeva, Tatiana, additional, Wang, Yiyan, additional, Palmer, Drake, additional, Ford, Rhodes, additional, Schamus, Sandra, additional, Singh, Ankita, additional, Lemon, Kathryn, additional, Kammula, Udai, additional, Vendetti, Frank P, additional, Poholek, Amanda, additional, Osmanbeyoglu, Hatice, additional, Delgoffe, Greg M., additional, and Bakkenist, Christopher J, additional

Published
2020
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