465 results on '"Wang Yuanhong"'
Search Results
202. A new algorithm for extracting center path in medical visualization.
- Author
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Peng Yanjun, Wang Yuanhong, and Shi Jiaoying
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- 2012
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203. Design and implementation of web services based GPRS automatic meter reading system.
- Author
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Wu Kehe, Zhang Xiaoliang, Wang Yuanhong, and Xu Yuhan
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- 2010
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204. Pathologic Quiz Case: A Large Pelvic Mass in a 44-Year-Old Man
- Author
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Wang, Yuanhong, primary and Sharkey, Francis E., additional
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- 2003
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205. Angiotensin II Evokes Calcium-Mediated Signaling Events in Isolated Dog Pancreatic Epithelial Cells
- Author
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Fink, Aaron S., primary, Wang, Yuanhong, additional, Mendez, Tatiana, additional, Worrell, Roger T., additional, Eaton, Douglas, additional, Nguyen, Toan D., additional, and Lee, Sum P., additional
- Published
- 2002
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206. Insulin Inhibits Secretin-Induced Pancreatic Bicarbonate Output Via Cholinergic Mechanisms
- Author
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Howard-McNatt, Marissa, primary, Simon, Timothy, additional, Wang, Yuanhong, additional, and Fink, Aaron S., additional
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- 2002
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207. Angiotensin (Angll) evokes calcium-mediated signalling events in isolated dog pancreatic duct epithelial (DPDE) cells
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Fink, Aaron S., primary, Wang, Yuanhong, additional, Worrell, Roger T., additional, Eaton, Douglas C., additional, Nguyen, Toan D., additional, and Lee, Sum P., additional
- Published
- 2001
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208. Microporous Polyoxometalates POMs/SiO2: Synthesis and Photocatalytic Degradation of Aqueous Organocholorine Pesticides
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Guo, Yihang, primary, Wang, Yuanhong, additional, Hu, Changwen, additional, Wang, Yonghui, additional, Wang, Enbo, additional, Zhou, Yongchun, additional, and Feng, Shouhua, additional
- Published
- 2000
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209. Insulin influences chloride conductance in isolated dog pancreatic duct epithelial (DPDE) cells
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Fink, Aaron S., primary, Wang, Yuanhong, additional, Savard, Chris E., additional, Nguyen, Toan D., additional, and Lee, Sum P., additional
- Published
- 2000
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210. Novel branch patterns and anticoagulant activity of glycosaminoglycan from sea cucumber Apostichopus japonicus.
- Author
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Yang, Jie, Wang, Yuanhong, Jiang, Tingfu, and Lv, Zhihua
- Subjects
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APOSTICHOPUS japonicus , *GLYCOSAMINOGLYCANS , *ANTICOAGULANTS , *SEA cucumbers , *POLYSACCHARIDES - Abstract
A novel glucosidic pattern of fucose branches was found in the glycosaminoglycan from the sea cucumber Apostichopus japonicus in China. The methylation of desulfated/carboxyl-reduced polysaccharides and analysis of unsaturated disaccharides generated from the enzymolysis of the defucosed polysaccharides demonstrated that the branch is formed by one fucopyranosyl residue, 46.5% of which is linked through the O-3 position of β- d -glucuronic acid, while 8.7% and 43.9% are linked through the O-6 and O-4 positions of the N -acetylgalactosamine moiety. The β- d -glucuronic acid, N -acetyl-β- d -galactosamine, α- l -fucose and sulfate ester with the molecular ratio of 0.97:1.00:1.13:3.85 composed the backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches. The sulfation patterns of fucose branches and the linkage pattern of the backbone structure were determined by 1/2 dimension NMR. The most abundant branch species were 2,4-di- O -sulfated and 3,4-di- O -sulfated fucose, but 4-mono- O -sulfated residue was also present. The structure of presently obtained glycosaminoglycan is different from that previously obtained from Stichopus japonicus (Kariya et al., Carbohyd. Res. 297 (1997) 273–279), which suggests that the structures of glycosaminoglycans from the same species of different regions somehow differ. The anticoagulant assay indicated that the polysaccharide possessed a high anticoagulant activity and the sulfated fucose branches were essential to the activity. [ABSTRACT FROM AUTHOR] more...
- Published
- 2015
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211. Depolymerized glycosaminoglycan and its anticoagulant activities from sea cucumber Apostichopus japonicus.
- Author
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Yang, Jie, Wang, Yuanhong, Jiang, Tingfu, Lv, Lv, Zhang, Boyuan, and Lv, Zhihua
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DEPOLYMERIZATION , *GLYCOSAMINOGLYCANS , *ANTICOAGULANTS , *SEA cucumbers , *APOSTICHOPUS japonicus , *CATALYTIC activity - Abstract
A controlled Cu 2+ catalytic free-radical depolymerization process of fucosylated chondroitin sulfate from sea cucumber Apostichopus japonicus was established. The results showed a good linear relationship between 1/ M w and time during the depolymerization. A series of fractions with different molecular weight were obtained, and the physicochemical properties of them were investigated and compared utilizing the chemical method, IR spectra and NMR spectra. The results showed no significant variations of the backbone and branches structures during the depolymerization. Furthermore, the anticoagulant activities of the depolymerized fractions were evaluated by the activated partial thromboplastin time (APTT). The APTT decreases in proportion to the molecular weight following a linear relationship and the prolongation of APTT activity requires at least oligosaccharide of 4 trisaccharide units (about 4000 Da). Their anticoagulant activity of low molecular weight fraction ( M w = 24,755 Da) is similar to LMWH with significantly less bleeding risk. The results suggest that the low molecular weight fraction could be used as a novel anticoagulant with less undesired side effects. [ABSTRACT FROM AUTHOR] more...
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- 2015
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212. Effects of sulfate chitosan derivatives on nonalcoholic fatty liver disease.
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Yu, Mingming, Wang, Yuanhong, Jiang, Tingfu, and Lv, Zhihua
- Abstract
Sulfate chitosan derivatives have good solubility and therapeutic effect on the cell model of NAFLD. The aim of this study was to examine the therapeutic effect of sulfate chitosan derivatives on NAFLD. The male Wistar rats were orally fed high fat emulsion and received sulfate chitosan derivatives for 5 weeks to determine the pre-treatment effect of sulfate chitosan derivatives on NAFLD. To evaluate the therapeutic effect of sulfate chitosan derivatives on NAFLD, the rats were orally fed with high concentration emulsion for 5 weeks, followed by sulfate chitosan derivatives for 3 weeks. Histological analysis and biomedical assays showed that sulfate chitosan derivatives can dramatically prevent the development of hepatic steatosis in hepatocyte cells. In animal studies, pre-treatment and treatment with sulfate chitosan derivatives significantly protected against hepatic steatohepatitis induced by high fat diet according to histological analysis. Furthermore, increased TC, ALT, MDA, and LEP in NAFLD were significantly ameliorated by pre-treatment and treatment with sulfate chitosan derivatives. Furthermore, increased TG, AST, and TNF-α in NAFLD were significantly ameliorated by treatment with sulfate chitosan derivatives. Sulfate chitosan derivatives have good pre-treatment and therapeutic effect on NAFLD. [ABSTRACT FROM AUTHOR] more...
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- 2014
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213. Synergetic effect of polyoxoniobate and NiS as cocatalysts for enhanced photocatalytic H2 evolution on Cd0.65Zn0.35S.
- Author
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Ma, Lifang, Li, Fengyan, Sun, Zhixia, Liu, Miaomiao, Wang, Yuanhong, and Xu, Lin
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- 2014
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214. Stability of the mitochondrial genome requires an amino-terminal domain of yeast mitochondrial RNA polymerase
- Author
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Wang, Yuanhong, primary and Shadel, Gerald S., additional
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- 1999
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215. Surface tension measurement of Ti–Zr and Ti–Hf alloys at high-temperature by the pendant drop method.
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Long, Jialiang, Wang, Yuanhong, Zeng, Yi, Xiong, Xiang, Li, Xi, Lun, Huilin, Ye, Ziming, Hu, Jinrun, Gao, Sen, Chen, Shiyan, Yang, Ge, Li, Rui, Li, Tianyou, and Zhang, Jiancheng
- Subjects
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SURFACE tension measurement , *SURFACE tension , *BINARY metallic systems , *ALLOYS , *MELT infiltration , *ULTRA-high-temperature ceramics , *LIQUID alloys - Abstract
High-temperature alloy melts introduced into carbon/carbon (C/C) composites by reactive melt infiltration (RMI) to form ultra-high-temperature ceramics (UHTCs) is currently an important method for improving their ablation resistance. As a fundamental thermo-property, surface tension has an important impact on the infiltration of alloy melts into the C/C composites. However, the surface tension of binary alloy systems, such as Ti–Zr and Ti–Hf, used for RMI has been little studied. In this work, experimental measurements through the pendant drop method and theoretical calculations of the surface tension of Ti–Zr and Ti–Hf alloys with different compositions have been carried out. Meanwhile, inevitably, impurities are introduced during the determination of surface tension at high temperatures, mainly carbon and oxygen. The effect of impurities on the phase composition, distribution, and surface tension of the alloys is investigated. The results demonstrated that the experimental measurements are in good agreement with the theoretical values of Butler's equation. The surface tension of Ti–Zr and Ti–Hf alloys increases with the content of Zr or Hf at the same temperature. Small amounts of oxygen are mainly dissolved in the lattice and have no significant effect on the surface tension. Carbon slightly decreases the surface tension of the alloy. • Experimental measurement and theoretical calculation of surface tension of Ti–Zr and Ti–Hf alloys for the first time. • The surface tension of Ti–Zr and Ti–Hf alloys increases with the content of Zr or Hf at the same temperature. • Carbon decreases the surface tension of the alloy. [ABSTRACT FROM AUTHOR] more...
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- 2022
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216. Experience report: using design patterns in the development of JB system.
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Wang Yuanhong, Mei Hong, and Shao Weizhong
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- 1997
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217. Effect observation on fumigation first then oral administration with Chinese medicine combined with acupoint massage for improvement of dry eye in patients with dry syndrome.
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Wang Yuanhong, Li Li, and Ji Wei
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- 2016
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218. Few-layer ZrTe3 nanosheets for ultrashort pulse mode-locked laser in 1.55 μm region.
- Author
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Hui, Zhanqiang, Wang, Yuanhong, Shen, Niping, Han, Dongdong, Li, Xiaohui, and Gong, Jiamin
- Subjects
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ULTRA-short pulsed lasers , *MODE-locked lasers , *OPTICAL saturable absorption , *ULTRASHORT laser pulses , *OPTICAL fiber communication , *RING lasers - Abstract
As a new two-dimensional (2D) material, zirconium telluride (ZrTe 3) is a typical charge density wave (CDW) material with strong electrical and optical anisotropy compared with graphene, transition metal disulfides (TMDs), topological insulators (TIs), and black phosphorus (BPs). To date, the nonlinear optical properties of ZrTe 3 and its applications in information photonics aspect have not been reported. In this work, the nonlinear optical saturable absorption properties of ZrTe 3 nanosheets and their application in passively mode-locked fiber laser are systematically investigated for the first time. The results show that the modulation depth and saturation intensity are 6.5% and 89.87 MW/cm2 at the optical fiber communication band (C band), respectively. In the erbium-doped fiber (EDF) ring laser with ZrTe 3 nanosheets as saturable absorber (SA), a stable mode-locked pulse train is obtained with the central wavelength of 1562.12 nm, the 3 dB optical spectral width of 2.07 nm, the basic repetition rate of 3.377 MHz, and the pulse duration of 1.469 ps when the pump power is 65 mW. Moreover, the dynamic evolution process of the optical spectrum and pulse sequence under different pump power are illustrated. All results reveal that ZrTe 3 has excellent potential for applying in many fields, including ultra-fast all-optical sampling, optical frequency comb, optical fiber communication, supercontinuum, etc, and provides an intuition of opening new avenues toward various advanced information photonic devices. • It is the first time to focused on the nonlinear optical properties of ZrTe 3 and its application in passively mode-locked fiber laser. • The results show that the modulation depth and saturation intensity are 6.5% and 89.87 MW/cm2 at the optical fiber communication band (C band) respectively. • In the erbium-doped fiber (EDF) ring laser with ZrTe 3 nanosheets as saturable absorber (SA), a stable mode-locked pulse train is obtained with the central wavelength of 1562.12 nm, the 3 dB optical spectral width of 2.07 nm, the basic repetition rate of 3.377 MHz, and the pulse duration of 1.469 ps when the pump power is 65 mW. • All results reveal that ZrTe 3 has great potential for applying in many fields including ultra-fast all-optical sampling, optical communication, etc, and provides an intuition of opening new avenues toward advanced information photonic devices. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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219. A multiplex nanoparticle-assisted polymerase chain reaction assay for detecting three canine epidemic viruses using a dual priming oligonucleotide system.
- Author
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Wang, Yong, Wang, Yuanhong, Chen, Zongyan, Liu, Guangqing, Jiang, Shudong, and Li, Chuanfeng
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POLYMERASE chain reaction , *CANINE distemper virus , *MIXED infections , *CANINE parvovirus - Abstract
• The method is the first time that DPO system and nanoPCR assay was combined together. • The assay was 100-fold more sensitive than the conventional PCR assay. • The assay was an alternative effective method for detecting mixed infected samples. • The assay was more specific than the traditional multiplex PCR method. A rapid and accurate diagnosis of mixed viral infections is important for providing timely therapeutic interventions. The aim of this study was to develop a highly sensitive and specific method for the simultaneous detection of canine distemper virus (CDV), canine parvovirus (CPV) and canine coronavirus (CCV) in mixed infections by combining the high specificity of a dual priming oligonucleotide (DPO) primer system with the high sensitivity of a nanoparticle-assisted PCR (nanoPCR) assay. Under the optimised assay conditions, the multiplex DPO-nanoPCR assay developed using DPO primers was 100-fold more sensitive than the multiplex PCR assay using conventional primers. The detection limits of the multiplex DPO-nanoPCR assay for the recombinant plasmids containing the cloned CDV, CPV and CCV target sequences were 5.4 × 102, 6.5 × 102 and 1.6 × 102 copies in a 25 μL assay, respectively. No cross-reaction with other canine viruses was observed. This is the first reported use of a multiplex nanoPCR assay with the DPO primer system for the simultaneous detection of CDV, CPV and CCV in mixed infections. The high sensitivity and specificity of the assay indicated its potential for use in clinical diagnosis and field surveillance of animal epidemics. [ABSTRACT FROM AUTHOR] more...
- Published
- 2021
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220. Facile Route to Synthesize Cu, S, N-Doped Carbon as Highly Efficient and Durable Electrocatalyst Towards Oxygen Reduction Reaction.
- Author
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Zhao, Yiwei, Yu, Yue, Wang, Yuanhong, Ma, Jicheng, and Xing, Shuangxi
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OXYGEN reduction , *CARBON , *CATALYSTS , *ATOMS , *ELECTROCATALYSIS - Abstract
This paper reports the generation of a type of carbon-based catalyst co-doped by Cu, S and N as efficient ORR catalysts in alkaline media. The electrocatalyst performs superior activity for ORR with onset and a half-wave potential (E1/2) of 0.945 V and 0.847 V, respectively, in 0.1 M KOH, comparable with Pt/C (0.955 V and 0.88 V). Especially, a current density of 5.55 mA cm−2 is achieved, rivaling that of the commercial Pt/C (5.21 mA cm−2). The high ORR activity is attributed to the positive effects of the different doping elements that introduce amounts of active sites, including defects, and efficient bonding between Cu, S, N and C atoms that contribute to ORR. Moreover, it shows great durability and strong methanol tolerance. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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221. Construction of sandwich-structured C/C-SiC and C/C-SiC-ZrC composites with good mechanical and anti-ablation properties.
- Author
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Zhao, Rida, Hu, Chenglong, Wang, Yuanhong, Pang, Shengyang, Li, Jian, Tang, Sufang, and Cheng, Hui-Ming
- Subjects
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SANDWICH construction (Materials) , *ELECTROMAGNETIC coupling , *FLEXURAL strength - Abstract
Four kinds of sandwich-structured C/C-SiC and C/C-SiC-ZrC composites with or without a SiC interphase deposited by isothermal chemical vapor infiltration (ICVI), were designed and fabricated by a joint process of electromagnetic coupling chemical vapor infiltration (ECVI) and precursor infiltration and pyrolysis (PIP). The fabricated composites are macroscopically nonhomogeneous materials with low density, high strength and low ablation rate. The interphase and matrix constituents had remarkable effects on the mechanical and ablation properties of these composites. The C/C-SiC composites with an ICVI-SiC interphase exhibited the highest flexural strength of 306.5 MPa. While the C/C-SiC-ZrC composites with the interphase showed the best anti-ablation performance with low linear and mass ablation rates of 0.37 μm/s and 0.04 mg/cm2·s, respectively, after the ablation for 500 s under an oxyacetylene flame test at around 2000 °C. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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222. Glycosaminoglycan from Apostichopus japonicus Improves Glucose Metabolism in the Liver of Insulin Resistant Mice.
- Author
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Chen, Yunmei, Wang, Yuanhong, Yang, Shuang, Yu, Mingming, Jiang, Tingfu, and Lv, Zhihua
- Abstract
Holothurian glycosaminoglycan isolated from Apostichopus japonicus (named AHG) can suppress hepatic glucose production in insulin resistant hepatocytes, but its effects on glucose metabolism in vivo are unknown. The present study was conducted to investigate the effects of AHG on hyperglycemia in the liver of insulin resistant mice induced by a high-fat diet (HFD) for 12 weeks. The results demonstrated that AHG supplementation apparently reduced body weight, blood glucose level, and serum insulin content in a dose-dependent manner in HFD-fed mice. The protein levels and gene expression of gluconeogenesis rate-limiting enzymes G6Pase and PEPCK were remarkedly suppressed in the insulin resistant liver. In addition, although the total expression of IRS1, Akt, and AMPK in the insulin resistant liver was not affected by AHG supplementation, the phosphorylation of IRS1, Akt, and AMPK were clearly elevated by AHG treatment. These results suggest that AHG could be a promising natural marine product for the development of an antihyperglycemic agent. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
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223. Autophagy receptor ZmNBR1 promotes the autophagic degradation of ZmBRI1a and enhances drought tolerance in maize.
- Author
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Xiang, Yang, Li, Guangdong, Li, Qian, Niu, Yingxue, Pan, Yitian, Cheng, Yuan, Bian, Xiangli, Zhao, Chongyang, Wang, Yuanhong, and Zhang, Aying
- Subjects
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DROUGHT tolerance , *AUTOPHAGY , *CORN , *BRCA genes , *POLYMERASE chain reaction , *MOLECULAR cloning - Abstract
Drought stress is a crucial environmental factor that limits plant growth, development, and productivity. Autophagy of misfolded proteins can help alleviate the damage caused in plants experiencing drought. However, the mechanism of autophagy‐mediated drought tolerance in plants remains largely unknown. Here, we cloned the gene for a maize (Zea mays) selective autophagy receptor, NEXT TO BRCA1 GENE 1 (ZmNBR1), and identified its role in the response to drought stress. We observed that drought stress increased the accumulation of autophagosomes. RNA sequencing and reverse transcription‐quantitative polymerase chain reaction showed that ZmNBR1 is markedly induced by drought stress. ZmNBR1 overexpression enhanced drought tolerance, while its knockdown reduced drought tolerance in maize. Our results established that ZmNBR1 mediates the increase in autophagosomes and autophagic activity under drought stress. ZmNBR1 also affects the expression of genes related to autophagy under drought stress. Moreover, we determined that BRASSINOSTEROID INSENSITIVE 1A (ZmBRI1a), a brassinosteroid receptor of the BRI1‐like family, interacts with ZmNBR1. Phenotype analysis showed that ZmBRI1a negatively regulates drought tolerance in maize, and genetic analysis indicated that ZmNBR1 acts upstream of ZmBRI1a in regulating drought tolerance. Furthermore, ZmNBR1 facilitates the autophagic degradation of ZmBRI1a under drought stress. Taken together, our results reveal that ZmNBR1 regulates the expression of autophagy‐related genes, thereby increasing autophagic activity and promoting the autophagic degradation of ZmBRI1a under drought stress, thus enhancing drought tolerance in maize. These findings provide new insights into the autophagy degradation of brassinosteroid signaling components by the autophagy receptor NBR1 under drought stress. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
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224. An equivalent diffusion coefficient model of the oxidation of ceramic matrix composites.
- Author
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Yu, Guoqiang, Shi, Xiaoting, Wang, Yuanhong, Du, Jinkang, Ni, Zheng, Gao, Xiguang, Wang, Fang, and Song, Yingdong
- Subjects
- *
AERODYNAMIC load , *GAS distribution , *OXIDATION kinetics , *DIFFUSION processes , *TENSILE tests , *CERAMIC-matrix composites , *DIFFUSION coefficients , *DIFFUSION - Abstract
A multiscale analysis method was developed to simulate the reaction-diffusion process of SiC/SiC composite structures in a coupled thermo-chemo-mechanical environment. For the diffusion process, the airflow channel was characterized by an equivalent diffusion coefficient model, which was established in this study. The corresponding calculation was developed using a numerical analysis method to obtain the gas concentration distribution in the structure. For the reaction process, an oxidation kinetics model was adopted to calculate morphological evolution of the airflow channel. The equivalent diffusion coefficient was then updated, and the reaction–diffusion coupling calculation was performed through an iterative scheme. To validate the model, an oxidation tensile test was conducted at 1200 °C. Finally, an adjustment sheet model was calculated as an example to analyze its reaction-diffusion process, proving that this method can be applied to SiC/SiC components under complex aerodynamic and temperature loads. [ABSTRACT FROM AUTHOR] more...
- Published
- 2021
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225. The dominant factor of C/SiC composites in ablation environment with heat and particle flow.
- Author
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Xiao, Keda, Zhang, Jiancheng, Wang, Yuanhong, Zeng, Yi, Xiong, Xiang, Hu, Jinrun, Li, Tianyou, Gao, Sen, Chen, Shiyan, Yang, Ge, Jiang, Tianxin, and Gao, Meng
- Subjects
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GRANULAR flow , *ALUMINUM oxide , *PLASMA flow , *SCRAMJET engines , *ATRIAL flutter - Abstract
As thermal structure components in scramjet engines, carbon-reinforced silicon carbide (C/SiC) composites are often subject to an ablation coupled with heat and particle flows. This study simulates the working environment of the scramjet engine's thermal structure component by coupling an Al 2 O 3 particle flow with a plasma flow. The ablation behaviour of C/SiC composites in the presence and absence of particle impact have been comprehensively analysed. The results show that the factor dominating the ablation behaviour varies with temperature. At relatively low temperatures, the particles have difficulty breaking down the dense SiO 2 layer formed from the oxidation of SiC in the presence of heat flow which is the dominant factor. As the temperature rises, the SiC begins to shift towards active oxidation. A few defects appear in the oxide layer, leading to a significant particle mechanical flaking effect. Particle flow is the dominant factor. However, a higher temperature led to the substantial active oxidation of SiC, and the oxide layer dissipated, exposing more defects. The heat flow causes a strong oxidation reaction, and the scouring effect of the particles is significant as well, both of which play a dominant role in the ablation process. • At lower ablation temperatures, SiC forms a dense oxide layer, with heat flow predominantly governing the ablation process. • As temperature rises, SiC undergoes active oxidation, resulting in defects within the oxide layer, where particle flow controls the ablation process. • At higher temperatures, both heat flow and particle flow jointly govern the ablation process. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
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226. An electrochemical approach capable of prostate specific antigen assay in human serum based on exonuclease-aided target recycling amplification.
- Author
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Miao, Peng, Jiang, Yiting, Wang, Yuanhong, Yin, Jian, and Tang, Yuguo
- Subjects
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TUMOR antigens , *ENZYME-linked immunosorbent assay , *ANTIGEN analysis , *IMMUNODIAGNOSIS , *IMMUNOENZYME technique - Abstract
In this report, an ultrasensitive electrochemical aptasensor for the detection of prostate specific antigen (PSA) in human serum is presented. DNA tetrahedron molecular layer is designed on the interface of a gold working electrode, which provides the recognition element for PSA capture. Next, exonuclease-aided target recycling amplification is performed, which determines the amount of attached silver nanoparticles (AgNPs) via silver-amino chemistry. By recording electrochemical responses from AgNPs, initial concentration of PSA can be easily evaluated. The limit of detection is pushed down to 0.11 pg mL −1 , which is attributed to the cyclic digestion effect of exonuclease and highly effective electrochemical reporters of AgNPs. The efficiency and robustness of this approach are further studied using human serum samples. The achieved results are satisfactory for future clinical applications. [ABSTRACT FROM AUTHOR] more...
- Published
- 2018
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227. Controllable silver embedding into polypyrrole.
- Author
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Zhang, Liang, Liu, Xianchun, Wang, Yuanhong, and Xing, Shuangxi
- Subjects
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POLYPYRROLE , *OXIDATION-reduction reaction , *NANOSTRUCTURED materials , *CHEMICAL processes , *CATALYSIS - Abstract
Eccentric encapsulation of silver by polypyrrole was realized via the direct redox reaction between AgNO 3 and pyrrole in the presence of sodium dodecyl sulfate. A facile method based on the relative amount of the Ag embedded in the normalized PPy sphere was developed and utilized to compare the interface tension between Ag, PPy and the solution. Via the aid of the embedding ratio, the formation of the eccentric silver@polypyrrole (Ag@PPy) was investigated by a temporal process and tuning the surfactant concentration and reaction temperature. Furthermore, such a unique nanostructure allowed the manipulation on the Ag end to achieve mushroom-like Au/Ag-PPy via the galvanic replacement reaction between Ag and HAuCl 4 , which was also dependent on the embedding ratio. After generating the above later nanostructure, much more active sites were available and much better catalytic activity was achieved taking the reduction of 4-nitrophenol by NaBH 4 as an example reaction. [ABSTRACT FROM AUTHOR] more...
- Published
- 2017
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228. Construction of a specific binding peptide based electrochemical approach for sensitive detection of Zn2 +.
- Author
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Liu, Tao, Yin, Jian, Wang, Yuanhong, and Miao, Peng
- Subjects
- *
ELECTROCHEMICAL sensors , *GOLD nanoparticles , *BINDING agents , *ZINC ions , *CARBON electrodes , *ELECTRIC conductivity - Abstract
A simple, highly sensitive, and cost-effective electrochemical method for the detection of Zn 2 + is constructed employing unmodified gold nanoparticles (AuNPs) and a specific binding peptide. AuNPs are firstly modified on a glassy carbon electrode, which show excellent electrical conductivity and provide a large superficial area for subsequent attachment of peptide with cysteine residues. Acidic amino acids residues of the peptide contribute to large electron transfer resistance, which is recorded by electrochemical impedance spectroscopy. Nevertheless, in the presence of Zn 2 + , the recognition sequence of the peptide (CCPGC) binds to Zn 2 + with a high affinity and is therefore not able to be modified on the electrode. By analyzing the decrease in electron transfer resistance, Zn 2 + level could be determined. The sensitivity of the proposed method is comparable to atomic absorption spectrometry and higher than most fluorescent, colorimetric and electrochemical sensors for Zn 2 + assay. Moreover, negligible interference is observed when detecting other ions. Therefore, the developed method has great potential environmental monitoring and clinical applications. [ABSTRACT FROM AUTHOR] more...
- Published
- 2016
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- View/download PDF
229. Electrochemical aptasensors for detection of small molecules, macromolecules, and cells.
- Author
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Han, Kun, Liu, Tao, Wang, Yuanhong, and Miao, Peng
- Subjects
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BIOSENSOR research , *ELECTROCHEMICAL analysis , *APTAMERS , *SMALL molecules , *MACROMOLECULES - Abstract
Aptamers are a kind of special nucleic acids that have the ability to bind various targets (e.g. small molecules, macromolecules, and cells) with high affinity and specificity. As a type of efficient recognition component in analytical designs and applications, aptamers have gained intense achievements in the designs of biosensors. Especially, the past few years have witnessed the fast development of electrochemical aptasensors. In this mini-review, we summarize recent progresses in electrochemical biosensors based on aptamers. Different targets and the corresponding detection principles are introduced. The methods to enhance detectable signals are mentioned including the employment of nanomaterials. Also, some possible limitations and future perspectives are discussed. [ABSTRACT FROM AUTHOR] more...
- Published
- 2016
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230. Biocontrol yeast T‐2 improves the postharvest disease resistance of grape by stimulation of the antioxidant system.
- Author
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Wu, Chenyang, Wang, Yuci, Ai, Dan, Li, Zhuoran, and Wang, Yuanhong
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POSTHARVEST diseases , *NATURAL immunity , *TABLE grapes , *BOTRYTIS cinerea , *YEAST , *GRAPE harvesting - Abstract
Table grapes are susceptible to external pathogens during postharvest storage. The resulting continuous oxidative stress causes damage and aging, thereby reducing the defense against disease. In this study, the effect of biocontrol yeast T‐2 on the storage performance of grapes was evaluated. After T‐2 treatment, the grapefruits rot rate and lesion diameter caused by Botrytis cinerea (B. cinerea) were significantly decreased at 2–5 days after inoculation (DAI). Additionally, the browning rate and shedding rate of grapefruit during storage were significantly reduced at 2–5 DAI, and the weight loss rate was significantly reduced at 3–5 DAI. The decreased malondialdehyde (MDA) content in grapefruits at 1–5 DAI with T‐2 indicated a reduction in oxidative damage. Furthermore, the activities of antioxidant enzymes such as peroxidase (POD), catalase (CAT), phenylalanin ammonia‐lyase (PAL) were significantly increased during most storage time after being treated with T‐2. Moreover, the contents of total phenolics and flavonoids and the expression levels of key enzyme genes in metabolic pathways were increased after T‐2 treatment during most postharvest storage time, providing evidence that T‐2 changed the biological process of phenolic flavonoid metabolism. The increase in enzymatic and nonenzymatic antioxidants after treatment with T‐2 reflected the strengthening of the antioxidant system, hence postponing fruit senescence and promoting storage performance under the stress of B. cinerea. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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231. Effect of siaD on Ag-8 to improve resistance to crown gall in grapes and related mechanisms.
- Author
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Ni, Xuemei, Li, Shiyu, Yuan, Yujin, Chang, Ruokui, Liu, Quanyong, Liu, Zhenxing, Li, Zhuoran, and Wang, Yuanhong
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COMPLEMENTATION (Genetics) , *COLONIZATION (Ecology) , *GRAPE industry , *AGROBACTERIUM tumefaciens , *DELETION mutation - Abstract
Crown gall caused by Agrobacterium vitis (A. vitis) is one of the crucial issues restricting the to grape industry. In this study, Agrobacterium tumefaciens (Ag-8) was separated from the soil that could prevent the occurrence of grape crown gall. By the mutagenesis of Ag-8 transposon, the siaD gene deletion strain (Δ siaD) showed significantly lower efficacy in grape and tomato plants for controlling grape crown gall, but the relevant mechanism was not clear. The biofilm formation and motility of Δ siaD were significantly decreased, and the colonization ability of Δ siaD in tomato roots was significantly reduced. RNA-seq analysis showed that the expression of nemR significantly reduced in the Δ siaD and that the expression of nemR showed a high correlation with biofilm and motility. Further studies showed that the nemR gene deletion strain of Ag-8 (Δ nemR) showed significantly reduced motility, biofilm formation and control of grape crown gall compared to Ag-8, and the nemR gene complementary strain of Ag-8 (Δ nemR -comp) recovered to Ag-8 wild-type levels. The inoculation experiments of preventive, curative or simultaneous treatment further showed that the preferential inoculation with Ag-8 reduced the incidence of grape crown gall on tomato plants, and studies showed that the mutation of siaD affected the site competition between Ag-8 and A. vitis , and that the mutation of nemR was consistent with the previous results. This study provides a new strategy for the prevention and control of grape crown gall, which is of great significance to the grape industry to increase production and income. • Agrobacterium tumefaciens (Ag-8) could prevent the occurrence of grape crown gall. • SiaD or nemR deletion of Ag-8 was critical for controlling grapevine crown galls. • Biofilm formation and colonization ability of Δ siaD were significantly decreased. • Expression of nemR showed a high correlation with biofilm and motility. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
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232. Metschnikowia Pulcherrima yeast T-2 VOCs enhances postharvest blueberry fruit resistance to Botrytis cinerea by activating flavonoid metabolic pathways.
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Li, Zhuoran, Liu, Quanyong, Wu, Chenyang, Yuan, Yujin, Ma, Zhaoqing, Chang, Ruokui, and Wang, Yuanhong
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BOTRYTIS cinerea , *FLAVONOIDS , *BLUEBERRIES , *VOLATILE organic compounds , *FRUIT , *FRUIT rots - Abstract
The postharvest rot of blueberry fruits caused by Botrytis cinerea led to economic losses. Here, we found that volatile organic compounds (VOCs) emitted by Metschnikowia Pulcherrima T-2 reduced fruit rot. Nevertheless, the mechanism of VOCs induced fruit resistance remains unclear. The total flavonoids content in blueberry fruits were significantly higher by T-2 VOCs treatment. Metabolomic analysis unraveled that the contents of quercetin, kaempferol and myricetin were significantly higher with T-2 VOCs treatment. The quercetin, kaempferol and myricetin also inhibited growth of B. cinerea in vitro. According to transcriptome analysis, the expression level of VcNAC83 was significantly highly increased after T-2 VOCs treatment. Furthermore, VcNAC83 was highly correlated with rot rate and total flavonoids content, and RT-qPCR also verified that VcNAC83 was significantly up-regulated. Transient overexpression of VcNAC83 in fruits enhanced the resistance to B. cinerea , and reduced lesion diameter and rot rate. The total flavonoids content, expression levels of flavonoid-related genes such as PAL , F3H , F3′H , DFR and F3′5′H and contents of quercetin, kaempferol and myricetin were also increased in the overexpressed blueberry fruits. These results support a critical role for T-2 VOCs in blueberry immune response to B. cinerea , and provide a new method of postharvest storage. • T-2 VOCs enhanced blueberry fruit resistance to Botrytis cinerea. • T-2VOCs increased the accumulation of flavonoids in blueberry fruits. • VcNAC83 participated in flavonoid biosynthesis. • VcNAC83 transient overexpression enhanced blueberry fruit resistance to B. cinerea. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
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233. Glycosaminoglycan from Apostichopus japonicus induces immunomodulatory activity in cyclophosphamide-treated mice and in macrophages.
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Wang, Han, Xu, Lei, Yu, Mingming, Wang, Yuanhong, Jiang, Tingfu, Yang, Shuang, and Lv, Zhihua
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APOSTICHOPUS japonicus , *MITOGEN-activated protein kinases , *IMMUNE system , *DISEASE resistance of plants , *GLYCOSAMINOGLYCANS , *HUMORAL immunity - Abstract
This study was designed to systematically elucidate the immunomodulation effect of glycosaminoglycan from Apostichopus japonicus (AHG) in cyclophosphamide (CY)-induced immunosuppression model and potential mechanism responsible for the activation of macrophages. The results showed that the treatment with AHG could increase natural killer (NK) cell cytotoxicity, carbon clearance and marker enzymes activities in CY-induced immunosuppression mice, indicating that the innate immunity experienced recovery to some extent. Moreover, CY-induced reductions in thymus and spleen indices, serum levels of cytokines, immunoglobulins and hemolysin, as well as the ratio of spleen lymphocyte subsets were recovered by AHG, suggesting that AHG could improve the adaptive immunity through cellular immunity and humoral immunity. Delightedly, it was found that AHG at 10 mg/kg body weight could restore the CY-induced immunosuppression in mice to normal level on both innate and adaptive immunity. Furthermore, AHG also promoted both the expression of NO, TNF-α, IL-6, IL-1β, IL-18 and MCP-1 protein and related mRNA in macrophages. It was revealed that AHG activated macrophages through the phosphorylation of mitogen-activated protein kinase (MAPK) and nuclear factor-B (NF-κB). In conclusion, AHG exerts remarkable immunomodulatory activities in both innate and adaptive immune system. These findings should have great value for further study on the immunopotentiating mechanisms of this biomacromolecule. [ABSTRACT FROM AUTHOR] more...
- Published
- 2019
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234. MiR-155 targets PTCH1 to mediate endothelial progenitor cell dysfunction caused by high glucose.
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Gao, Jie, Zhao, Gang, Li, Wei, Zhang, Jiayuan, Che, Yanling, Song, Meiyu, Gao, Shan, Zeng, Bin, and Wang, Yuanhong
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MICRORNA , *PROGENITOR cells , *HYPERGLYCEMIA , *DIABETES complications , *WOUND healing , *LABORATORY rats - Abstract
Endothelial progenitor cells (EPCs) are involved in diabetes-associated complications, including diabetic foot ulcer (DFU). Recent reports showed that miR-155 downregulation promotes wound healing in diabetic rats and ameliorates endothelial injury induced by high glucose, but its role in DFU is unknown. We found that miR-155 was overexpressed in EPCs from patients with DFU and in high glucose–induced EPCs from healthy people. Reductions in cell viability, migration, tube formation and nitric oxide production, as well as increases in lactated hydrogenase, cell apoptosis, and reactive oxygen species induced by high glucose, were enhanced by miR-155 overexpression and restrained by miR-155 inhibition. Additionally, dual-luciferase reporter assay demonstrated that miR-155 directly targeted the 3′ untranslated region of patched-1 (PTCH1), a receptor of the sonic hedgehog signaling pathway, and downregulated the mRNA and protein expression of PTCH1. qRT-PCR and Western blot results revealed that the PTCH1 was downregulated in EPCs treated with high glucose. Silencing PTCH1 by PTCH1 siRNA alleviated the protective effect of anti-miR-155 on high glucose–induced EPC dysfunction. Our results indicate that miR-155 worsened high glucose–induced EPC function by downregulating PTCH1. These findings suggest that miR-155 may be a potential therapeutic target for DFU. [ABSTRACT FROM AUTHOR] more...
- Published
- 2018
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235. Confined polyaniline derived mesoporous carbon for oxygen reduction reaction.
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Xing, Shuangxi, Yu, Xiaodan, Wang, Guibao, Yu, Yue, Wang, Yuanhong, and Xing, Yan
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POLYANILINES , *MESOPOROUS materials , *OXYGEN reduction , *CARBONIZATION , *NANOSTRUCTURED materials - Abstract
Based on the previous generation of yolk-shell nanostructured polyaniline@SiO 2 particles, a confined carbonization process followed by etching the SiO 2 layer is carried out to achieve carbon particles. The existence of the SiO 2 shell helps to preserve the original well-dispersed morphology of the polyaniline that retains amounts of active sites for oxygen reduction reaction. Furthermore, the CeO 2 oxidant and the SiO 2 shell lead to the increased amount of oxygen existing in the product and the oxygen reduction reaction results show the well-maintained nanostructure with mesopores and the effective O doping benefit for achieving enhanced catalytic activity. [ABSTRACT FROM AUTHOR] more...
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- 2017
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236. Collaborative filtering with weighted opinion aspects.
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Yang, Chong, Yu, Xiaohui, Liu, Yang, Nie, Yanping, and Wang, Yuanhong
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SENTIMENT analysis , *INFORMATION filtering , *RATINGS of computers , *FACTORIZATION , *CALCULUS of tensors , *COMPUTER networks - Abstract
Collaborative filtering (CF) aims to produce recommendations based on other users' ratings to an item. Most existing CF methods rely on the overall ratings an item has received. However, these ratings alone sometimes cannot provide sufficient information to understand users' behaviors. For example, a user giving a high rating may indicate that he loves the item as a whole; however, it is still likely that he dislikes some particular aspects at the same time. In addition, users tend to place different emphases on different aspects when reaching an overall rating. This emphasis on aspects may even vary from users to items, and has a significant impact on a user's final decision. To make a better understanding of a user' behavior and generate a more accurate recommendation, we propose a framework that incorporates both user opinions and preferences on different aspects. This framework is composed of three components, namely, an opinion mining component, an aspect weighting computing component, and a rating inference component. The first component exploits opinion mining techniques to extract and summarize opinions on multiple aspects from reviews, and generates ratings on various aspects. The second component applies a tensor factorization strategy to automatically infer weights of different aspects in reaching an overall rating. The last one infers the overall rating of an item based on both aspect ratings and weights. Experiments on two real datasets prove that our model performs better compared with the baseline methods. [ABSTRACT FROM AUTHOR] more...
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- 2016
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237. Facile route to achieve hierarchical hollow MnO2 nanostructures.
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Gao, Li, Zhang, Linlin, Jia, Shuyan, Liu, Xianchun, Wang, Yuanhong, and Xing, Shuangxi
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MANGANESE dioxide , *NANOSTRUCTURED materials , *FABRICATION (Manufacturing) , *POLYANILINES , *CHEMICAL templates , *CHEMICAL reduction - Abstract
Hierarchical hollow nanostructured MnO 2 has been fabricated using polyaniline spheres as reactive templates. The reaction between KMnO 4 and polyaniline leads to the reduction of KMnO 4 into Mn 2+ ions that are further oxidized by excessive KMnO 4 into MnO 2 layers. Meanwhile, the polyaniline cores are over-oxidized and diffuse into the solution to form the cavity. The ratio of the KMnO 4 and polyaniline plays an important role in achieving the hollow structure via establishing a reasonable diffusion/reaction rate equilibrium. Owing to the unique nanostructure, the MnO 2 electrode exhibits a specific capacitance of 308 F g −1 at a current density of 0.2 A g −1 and maintains 91% capacitance value after 3000 cycles at a current density of 2 A g −1 . An asymmetric supercapacitor assembled by the hollow MnO 2 and activated carbon presents energy densities of 20.93∼7.23 W h kg −1 under the power densities of 180∼9000 W kg −1 and 24% capacitance fading is detected after 2000 cycles at a current density of 4 A g −1 . [ABSTRACT FROM AUTHOR] more...
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- 2016
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238. Discovery of evodiamine derivatives as potent insecticide candidates.
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Liu, Jingbo, Shi, Yabing, Chen, Shuting, Li, Fengyun, Wen, Wen, and Wang, Yuanhong
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INSECTICIDES , *STRUCTURE-activity relationships , *RYANODINE receptors , *DIAMONDBACK moth , *HELICOVERPA armigera , *NATURAL products - Abstract
Evodiamine derivative 3z exhibited excellent larvicidal activities against M. separata , P. xylostella and H. armigera, and could act on the ryanodine receptor of M. separata. [Display omitted] • A series of evodiamine derivatives were rationally designed and synthesized. • 3z exhibited excellent larvicidal activities, and could be a promising potent insecticide candidate. • F atom at the E ring of evodiamine had an important effect on the larvicidal activity. • The ryanodine receptor of Mythimna separata is the potential target of 3z. In the search for novel more effective insecticides, natural products could be used as ideal template compounds due to their good environmental compatibility, various bioactivities, unique scaffolds and mode of action. We have found that natural product evodiamine, the main active component from the fruits of Evodia rutaecarpa (Juss.) Benth , displayed obvious insecticidal activities against lepidoptera pests. To continue our research, a series of evodiamine derivatives 3a-3aa were rationally designed and synthesized. The larvicidal activities results indicated that most of target compounds displayed better efficacy than evodiamine, matrine, and rotenone against Mythimna separata, Plutella xylostella and Helicoverpa armigera, among which 3z exhibited excellent larvicidal activities (65% at 2.5 mg/L against M. separata, 75% at 1.0 mg/L against P. xylostella , and 85% 10 mg/L against H. armigera , respectively), much better than evodiamine (0%), matrine (0%), and rotenone (0%). The preliminary structure activity relationships demonstrated that the fluorine atom at the E ring of evodiamine had a positive influence on the larvicidal activity. The calcium imaging experiment studies indicated that 3z could act on the ryanodine receptor (RyR) of M. separata and was an effective calcium activator for RyR. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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239. Discovery of a polysubstituted phenyl containing novel N-phenylpyrazole scaffold as potent ryanodine receptor activator.
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Liu, Jingbo, Li, Fengyun, Hao, Zesheng, Wang, Yuanhong, Hua, Xuewen, Li, Yuxin, and Li, Zhengming
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PLUTELLIDAE , *DIAMONDBACK moth , *CHLORANTRANILIPROLE , *TRIAZINE derivatives , *CALCIUM compounds , *RYANODINE receptors , *MOLECULAR docking , *ISOXAZOLINE - Abstract
• Novel N -phenylpyrazole derivatives containing a polysubstituted phenyl ring scaffold 7a-p were designed and synthesized. • LC 50 values of 7f and 7o against oriental armyworm were 8.83 × 10−2 and 7.12 × 10−2 mg L−1, respectively close to chlorantraniliprole (6.79 × 10−2 mg L−1). • Compound 7f showed 90% larvicidal activity against diamondback moth at 0.01 mg L−1, superior to chlorantraniliprole (50%) and cyantraniliprole (40%). • Molecular docking suggested the possible binding mode of the target compounds to the insect RyRs. • Calcium imaging experiment showed that insect RyRs is the potential target of the title compounds. To develop the novel ryanodine receptors (RyRs) insecticides, encouraged by our previous research work, a series of novel N -phenylpyrazole derivatives containing a polysubstituted phenyl ring scaffold were designed and synthesized. The bioassays results indicated that some title compounds exhibited excellent insecticidal activity. For oriental armyworm (Mythimna separata), compounds 7f , 7g , 7i and 7o at 0.5 mg L−1 displayed 100% larvicidal activity, and even at 0.1 mg L−1, 7o was 30% larvicidal activity, comparable to chlorantraniliprole (30%) and better than cyantraniliprole (10%). Compounds 7f and 7o had the median lethal concentrations (LC 50) of 8.83 × 10−2 and 7.12 × 10−2 mg L−1, respectively, close to chlorantraniliprole (6.79 × 10−2 mg L−1). Additionally, for diamondback moth (Plutella xylostella), the larvicidal activity of compounds 7f and 7i were 90% and 70% at 0.01 mg L−1, respectively, better than chlorantraniliprole (50%) and cyantraniliprole (40%). More impressively, the LC 50 value of 7f was 4.2 × 10−3 mg L−1, slightly lower than that of chlorantraniliprole (5.0 × 10−3 mg L−1). The molecular docking between compound 7f and RyRs of diamondback moth validated our molecular designation. Furthermore, the calcium imaging experiment explored the influence of compound 7o on the calcium homeostasis in the central neurons of the third larvae of oriental armyworm. The results of this study indicated that 7o is a potent novel lead targeting at RyRs. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
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240. The Rlm1 transcription factor in Candida oleophila contributes to abiotic stress resistance and biocontrol efficacy against postharvest gray mold of kiwifruit.
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Sui, Yuan, Sun, Zhiqiang, Zou, Yuping, Li, Wenhua, Jiang, Mingguo, Luo, Yuzhen, Liao, Wenjian, Wang, Yuanhong, Gao, Xuewen, Liu, Jia, and Wu, Huijun
- Subjects
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ABIOTIC stress , *TRANSCRIPTION factors , *KIWIFRUIT , *CANDIDA , *BOTRYTIS cinerea , *BIOLOGICAL pest control agents , *OXIDATIVE stress - Abstract
• Candida oleophila effectively controlled postharvest gray mold of kiwifruit. • Two Rlm1 mutants (Δ Rlm1-1 and Δ Rlm1-2) of C. oleophila were generated. • The two mutants were more sensitive to a variety of stresses, compared to WT. • The two mutants exhibited lower biocontrol efficacy against gray mold. • The study shed light on the function of Rlm1 in a postharvest biocontrol yeast. Biological control utilizing antagonistic yeasts has been actively pursued as an alternative to synthetic fungicides for the management of postharvest diseases. Abiotic stress resistance is an important attribute for antagonistic yeasts, directly associated with their biocontrol efficacy. The MADS-box transcription factor, Rlm1 , has been reported to regulate the response of model yeasts to cell wall stress. Rlm1 in the antagonistic yeast, Candida oleophila , was found to play a role in resistance to salt, heat, and oxidative stress. Two Rlm1 mutants (Δ Rlm1-1 and Δ Rlm1-2) were generated. Compared to the wild-type (WT), C. oleophila I-182, Δ Rlm1-1 , and Δ Rlm1-2 were more sensitive to a variety of stresses, including heat, salt, and oxidative stress. The mutants also exhibited lower biocontrol efficacy against gray mold caused by Botrytis cinerea , and slower growth in kiwifruit wounds with respect to the WT. This study provided the information to understand the relationship between the Rlm1 transcription factor, stress resistance, and biocontrol efficacy of antagonistic yeasts used for the biocontrol of postharvest diseases. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
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241. Fabrication and characterization of zein/tea saponin composite nanoparticles as delivery vehicles of lutein.
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Ma, Mengjie, Yuan, Yongkai, Yang, Shuang, Wang, Yuanhong, and Lv, Zhihua
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LUTEIN , *IONIC strength , *TEA , *HYDROPHOBIC interactions , *ELECTROSTATIC interaction , *HYDROGEN bonding - Abstract
The application of lutein hampers due to its environmental instability, poor water solubility and bioaccessibility. The goal of this paper was to overcome these challenges by fabricating zein/tea saponin nanoparticles act as the vehicles of lutein (ZTSLNPs). The optimized encapsulation efficiency of ZTSLNPs was 92.91%, while the loading capacity was 1.19%. The ZTSLNPs were smooth spherical structures with mean particle size of 213 nm and negative zeta-potential. Electrostatic interactions, hydrophobic interactions, and hydrogen bonding were the main forces to form ZTSLNPs. The encapsulated lutein in ZTSLNPs was amorphous. The secondary structure of zein was changed by incorporating lutein or tea saponin. ZTSLNPs showed great stability at pH 4.0–9.0, and exhibited excellent ionic strength stability and thermal stability. Meanwhile, the ZTSLNPs effectively reduced the decomposition of lutein during 15 days storage. Furthermore, ZTSLNPs showed excellent redispersibility. Besides, the water solubility and bioaccessibility of lutein were improved by ZTSLNPs. These findings indicated that the vehicle designed in our work would be a promising strategy to improving the application of hydrophobic functional factor. • A novel lutein delivery vehicle was fabricated using zein and tea saponin. • The addition of lutein or tea saponin led to the conformational change in zein. • Lutein-loaded zein/tea saponin nanoparticles showed good environmental stability. • Zein/tea saponin nanoparticles improved the solubility of lutein about 60 times. • The lutein in zein/tea saponin nanoparticles exhibited good bioaccessibility. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
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242. Sensitive detection of benzophenone-type ultraviolet filters in plastic food packaging materials by sheathless capillary electrophoresis–electrospray ionization–tandem mass spectrometry.
- Author
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Hu, Lihuan, Tian, Miaomiao, Feng, Wenxia, He, Huiyu, Wang, Yuanhong, and Yang, Li
- Subjects
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ULTRAVIOLET filters , *FOOD packaging , *PLASTICS in packaging , *PACKAGING materials , *MASS spectrometry , *ELECTROSPRAY ionization mass spectrometry , *TANDEM mass spectrometry - Abstract
• Sheathless CE–ESI–MS/MS analysis of BP-type UV filters in food packaging. • LOD as low as 7 pg/mL with accelerated solvent extraction and solid phase extraction. • Successful analysis of six BP-type UV filters in eight plastic food packaging films. While they are commonly used as ultraviolet (UV) filters in plastic food packaging materials, benzophenones (BPs) are reported as environmental endocrine disruptors, and some of them possess significant estrogenic activity. Therefore sensitive determination of the content of those UV filters in plastic polymers is of vital importance in safety assessment of food packaging materials. Here, the sheathless capillary electrophoresis–electrospray ionization–tandem mass spectrometry (CE–ESI–MS/MS) method is applied for the first time to sensitively detect BP-type UV filters in plastic food packaging materials. We investigate and optimize a variety of factors that may affect ESI–MS efficiency and CE separation. Sensitive detection of six BP-type UV filters is achieved using sheathless CE–ESI–MS/MS in conjunction with accelerated solvent extraction and solid phase extraction, with the limit-of-detection of 7 pg/mL–2.4 ng/mL. The method exhibits excellent inter/intra-day repeatability along with the advantages of efficient separation, rapid analysis, low sample consumption and high sensitivity. Six BP-type UV filters in eight different brands of plastic films obtained from supermarkets are successfully analyzed using the method. Good recoveries of 81.3–104.1% at three levels of spiked concentrations are achieved with low RSDs (n = 5) of 2.5–8.7%. Our study shows that the sheathless CE–ESI–MS/MS is a robust and reliable method for sensitive and rapid analysis of UV filters, which would be of potential application in safety assessment of plastic food packaging materials. [ABSTRACT FROM AUTHOR] more...
- Published
- 2019
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243. Therapeutic potential and pharmacological insights of total glucosides of paeony in dermatologic diseases: a comprehensive review.
- Author
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Wang H, Yu W, Wang T, Fang D, Wang Z, and Wang Y
- Abstract
Total glucosides of paeony (TGP) are a group of monoterpenes extracted from Paeonia lactiflora Pall. , primarily including metabolites such as paeoniflorin and oxypaeoniflorin. Modern pharmacological studies have shown that TGP possesses a variety of biological effects, including immunomodulatory, anti-inflammatory, hepatoprotective, nephroprotective, antidepressant, and cell proliferation regulatory activities. In recent years, clinical research has demonstrated favorable therapeutic effects of TGP on disorders of the liver, cardiovascular, nervous, endocrine, and skeletal systems. Particularly in dermatological treatments, TGP has been found to significantly improve clinical symptoms and shorten the course of the disease. However, there are still certain limitations in the scientific rigor of existing studies and in its clinical application. To assess the potential of TGP in treating dermatologic diseases, this article provides a review of its botanical sources, preparation and extraction processes, quality control, and major chemical metabolites, as well as its pharmacological research and clinical applications in dermatology. Additionally, the mechanisms of action, research gaps, and future directions for TGP in the treatment of dermatologic diseases are discussed, offering valuable guidance for future clinical research on TGP in dermatology., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Wang, Yu, Wang, Fang, Wang and Wang.) more...
- Published
- 2025
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244. Searches for exotic spin-dependent interactions with spin sensors.
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Jiang M, Su H, Chen Y, Jiao M, Huang Y, Wang Y, Rong X, Peng X, and Du J
- Abstract
Numerous theories have postulated the existence of exotic spin-dependent interactions beyond the Standard Model of particle physics. Spin-based quantum sensors, which utilize the quantum properties of spins to enhance measurement precision, emerge as powerful tools for probing these exotic interactions. These sensors encompass a wide range of technologies, such as optically pumped magnetometers, atomic comagnetometers, spin masers, nuclear magnetic resonance, spin amplifiers, and nitrogen-vacancy centers. These technologies stand out for their ultrahigh sensitivity, compact tabletop design, and cost-effectiveness, offering complementary approaches to the large-scale particle colliders and astrophysical observations. This article reviews the underlying physical principles of various spin sensors and highlights the recent theoretical and experimental progress in the searches for exotic spin-dependent interactions with these quantum sensors. Investigations covered include the exotic interactions of spins with ultralight dark matter, exotic spin-dependent forces, electric dipole moment, spin-gravity interactions, and among others. Ongoing and forthcoming experiments using advanced spin-based sensors to investigate exotic spin-dependent interactions are discussed., (Creative Commons Attribution license.) more...
- Published
- 2024
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245. A method of identification and localization of tea buds based on lightweight improved YOLOV5.
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Wang Y, Lu J, Wang Q, and Gao Z
- Abstract
The low degree of intelligence and standardization of tea bud picking, as well as laborious and time-consuming manual harvesting, bring significant challenges to the sustainable development of the high-quality tea industry. There is an urgent need to investigate the critical technologies of intelligent picking robots for tea. The complexity of the model requires high hardware computing resources, which limits the deployment of the tea bud detection model in tea-picking robots. Therefore, in this study, we propose the YOLOV5M-SBSD tea bud lightweight detection model to address the above issues. The Fuding white tea bud image dataset was established by collecting Fuding white tea images; then the lightweight network ShuffleNetV2 was used to replace the YOLOV5 backbone network; the up-sampling algorithm of YOLOV5 was optimized by using CARAFE modular structure, which increases the sensory field of the network while maintaining the lightweight; then BiFPN was used to achieve more efficient multi-scale feature fusion; and the introduction of the parameter-free attention SimAm to enhance the feature extraction ability of the model while not adding extra computation. The improved model was denoted as YOLOV5M-SBSD and compared and analyzed with other mainstream target detection models. Then, the YOLOV5M-SBSD recognition model is experimented on with the tea bud dataset, and the tea buds are recognized using YOLOV5M-SBSD. The experimental results show that the recognition accuracy of tea buds is 88.7%, the recall rate is 86.9%, and the average accuracy is 93.1%, which is 0.5% higher than the original YOLOV5M algorithm's accuracy, the average accuracy is 0.2% higher, the Size is reduced by 82.89%, and the Params, and GFlops are reduced by 83.7% and 85.6%, respectively. The improved algorithm has higher detection accuracy while reducing the amount of computation and parameters. Also, it reduces the dependence on hardware, provides a reference for deploying the tea bud target detection model in the natural environment of the tea garden, and has specific theoretical and practical significance for the identification and localization of the intelligent picking robot of tea buds., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Wang, Lu, Wang and Gao.) more...
- Published
- 2024
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246. New Constraints on Axion-Mediated Spin Interactions Using Magnetic Amplification.
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Su H, Jiang M, Wang Y, Huang Y, Kang X, Ji W, Peng X, and Budker D
- Abstract
Axions are highly motivated hypothetical particles beyond the standard model that can be dark matter candidates and address the strong CP problem. Here we search for axion-mediated interactions generated between two separated ^{129}Xe gas ensembles, monitoring and polarizing the ^{129}Xe nuclear spins through spin-exchange interactions with Rb vapor. Our method exploits the magnetic amplification through effective fields from Rb-Xe collisions, increasing the sensitivity for axion-mediated interactions by up to 145-fold relative to conventional methods. Moreover, we employ template filtering to extract exotic interactions with a maximum signal-to-noise ratio. By combining two techniques, axion-mediated interactions are constrained to be less than 10^{-5} of normal magnetic interactions on a length scale of 60 mm. We establish new constraints on the neutron-neutron pseudoscalar couplings for a mass range that expands into the well-motivated "axion window" (10 μeV-1 meV), improving previous constraints by up to 50-fold within it and 118-fold outside it. We further discuss promising applications in searches for other axion-nucleon interactions, including axion dark matter and black hole axion bursts with sensitivity well beyond astrophysical limits by several orders of magnitude. more...
- Published
- 2024
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247. First Report of Lelliottia amnigena Causing Soft Rot on Purple Stem Mustards in China.
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Li J, Qin S, Shi Y, Xie X, Chai AL, Wang Y, Li B, and Li L
- Abstract
The purple stem mustards (Brassica rapa subsp. chinensis var. purpuraria) (Govaerts R, 2003) are widely cultivated along Yangtze River Valley in China, which is famous for its flavorful and nutritious edible stalks (Wang et al., 2022). In February 2023, a disease of soft rot was observed in multiple purple stem mustards planting fields in Wuhan city, Hubei province, China (30.41°N, 114.22°E). Disease incidence rates were almost 20 to 30% in the planting area (5 ha in size), causing severe economic loss. Infected plants displayed water-soaked symptoms at the base of the petioles, emitting a foul soft rot odor. The severely infected petioles, stems and roots exhibit pus symptoms leading to plant death. To identify the causal agent, small pieces of soft rot symptomatic tissue were cut from the margin of necrotic lesions and surface disinfected with 75% (v/v) ethanol for 30 seconds, followed by three successive rinses with sterile distilled water. The exudates from the clipped tissues were serially diluted and then incubated onto nutrient agar (NA) plates to obtain purified strains at 28°C for 48 hours (Koike et al., 2002). After incubation, 15 strains were obtained and the colonies of all strains were Gram-negative, aerobic, small, round, convex, whitish to dull white, and had smooth slimy edges. Three single bacterial strains CT020801 - CT020803, which were individually isolated from three different diseased samples, were selected as representative strains for further study. Biochemical tests using the BIOLOG GENIII microplate system (Biolog, Hayward, CA, USA) revealed that these strains were positive for methyl red, pectin, dextrin, D-Cellobiose, β-galactosidase, citrate, and maltose, but negative for indole, arginine dihydrolase, urease, ornithine decarboxylase, and gelatinase tests. The 16S ribosomal RNA gene and the three housekeeping genes, atpD, rpoB, and recN were amplified using genomic DNA of Lelliottia amnigena NCTC12124T as the template, with specially designed primers. All amplified fragments were sequenced and deposited in GenBank with accession numbers OQ954706-OQ954707, OQ954713, and OQ953873-OQ953881. BLAST alignments of the 16S rRNA, atpD, rpoB and recN sequences revealed that the sequences of Strain CT020801-03 exhibited the highest identity (100%, ≥97.97%, ≥98.85% and ≥94.52%, respectively) with L. amnigena (Figure S2). Phylogenetic tree analysis based on multilocus sequence joint 16S rRNA - atpD - rpoB - recN revealed that CT020801 - CT020803 and L. amnigena clustered together in the same clade (Carrie et al., 2013). These results were consistent with those reported for Lelliottia amnigena (Osei et al., 2022). To confirm pathogenicity, healthy base petioles of three-week-old purple stem mustards seedlings were stab inoculated with 20 μL bacterial suspensions (approximately 108 CFU/mL) and then incubated at 28°C and 80% relative humidity in a growth chamber. A sterile liquid NB medium served as the negative control. The test was repeated thrice with each test consisting of five seedlings per treatment. After three days, soft rot symptoms appeared on the stem bases of the inoculated plants, consistent with the initial symptoms observed in the field. Control plants showed no symptoms. The strains were successfully re-isolated from symptomatic plants and identified as L. amnigena, fulfilling Koch's postulates. L. amnigena, a member of the Enterobacter genus (Birlutiu et al., 2023; Brady et al., 2013; Izard et al., 1981), is commonly found in soil and has been identified as an opportunistic pathogen responsible for plant soft rot disease (Reyes-García et al., 2020; Schroeder et al., 2009; Wu et al., 2023). Previous studies have linked L. amnigena to soft rot disease in potato tubers in China. However, this study marks the first documented case of L. amnigena causing soft rot disease in purple stem mustards in China. This discovery expands the known host range of the pathogenic bacteria and will help to provide essential information for making effective measures to manage this disease. more...
- Published
- 2024
- Full Text
- View/download PDF
248. Enhancing Quality of Life in Rheumatoid Arthritis Patients: A Combined Approach of Methotrexate and Pain-specific Nursing Intervention.
- Author
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Xu T, Liu Y, Chen D, Wu L, and Wang Y
- Subjects
- Humans, Female, Male, Middle Aged, Prospective Studies, Antirheumatic Agents therapeutic use, Adult, Aged, Pain Management methods, Pain Measurement, Cohort Studies, Arthritis, Rheumatoid drug therapy, Arthritis, Rheumatoid psychology, Arthritis, Rheumatoid complications, Methotrexate therapeutic use, Quality of Life psychology
- Abstract
Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder affecting joint health and patients' overall well-being. While methotrexate (MTX) is a standard therapeutic intervention, enhancing its efficacy with pain-specific nursing remains underexplored., Objective: This study aims to assess the impact of combining MTX with pain-specific nursing on patients with RA, providing valuable insights for clinical practice and offering an improved therapeutic approach to alleviate pain and enhance the overall quality of life for affected individuals., Methods: We conducted a prospective cohort study, choosing a cohort of 86 RA patients admitted to our hospital from March 2021 to March 2023. After treatment, we compared the number of swollen and painful joints, duration of morning stiffness, and scores on the Visual Analogue Scale (VAS), Pittsburgh Sleep Quality Index (PSQI), and Self-rating Anxiety Scale/Self-rating Depression Scale (SAS/SDS) between the two groups. Nursing satisfaction was surveyed upon discharge, and patient quality of life was assessed using the 36-item Short Form Health Survey (SF-36)., Results: The research group exhibited a notable decrease in the number of swollen and painful joints, significantly shorter morning stiffness duration, and marked reductions in VAS, PSQI, SAS, and SDS scores compared to the control group (P < .05). Additionally, nursing satisfaction and SF-36 scores were higher in the research group (P < .05)., Conclusions: The combination of MTX and pain-specific nursing effectively alleviated pain and improved the quality of life and nursing satisfaction among RA patients. more...
- Published
- 2024
249. Cooperative Spin Amplifier for Enhanced Quantum Sensing.
- Author
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Xu M, Jiang M, Wang Y, Su H, Huang Y, and Peng X
- Abstract
Quantum sensing is crucial for precision measurements, yet quantum sensor sensitivity is often limited by the coherence time of the quantum system. Here, we demonstrate a method to enhance coherence time through cooperative spins. Using a tunable feedback circuit, we induce cooperation among noble-gas ^{129}Xe spins, resulting in an impressive 18-fold coherence enhancement. Moreover, we show that the cooperative ^{129}Xe spins can significantly amplify magnetic signals by at least 3 orders of magnitude. Magnetic field sensing assisted with such a cooperative spin amplifier realizes the sensitivity of 4 fT/Hz^{1/2} and surpasses the spin-projection noise of the embedded ^{87}Rb spin gas magnetometer. These results pave the way for a new class of "cooperative quantum sensors," and open up exciting prospects in fundamental physics. more...
- Published
- 2024
- Full Text
- View/download PDF
250. New Classes of Magnetic Noise Self-Compensation Effects in Atomic Comagnetometer.
- Author
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Qin Y, Shao Z, Hong T, Wang Y, Jiang M, and Peng X
- Abstract
Precision measurements of anomalous spin-dependent interactions are often hindered by magnetic noise and other magnetic systematic effects. Atomic comagnetometers use the distinct spin precession of two species and have emerged as important tools for effectively mitigating the magnetic noise. Nevertheless, the operation of existing comagnetometers is limited to very low-frequency noise commonly below 1 Hz. Here, we report a new type of atomic comagnetometer based on a magnetic noise self-compensation mechanism originating from the destructive interference between alkali-metal and noble-gas spins. Our comagnetometer employing K-^{3}He system remarkably suppresses magnetic noise exceeding 2 orders of magnitude at higher frequencies up to 160 Hz. Moreover, we discover that the capability of our comagnetometer to suppress magnetic noise is spatially dependent on the orientation of the noise and can be conveniently controlled by adjusting the applied bias magnetic field. Our findings open up new possibilities for precision measurements, including enhancing the search sensitivity of spin-dark matter particles interactions into unexplored parameter space. more...
- Published
- 2024
- Full Text
- View/download PDF
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