201. A simple and pH-independent and ultrasensitive fluorescent probe for the rapid detection of Hg2+.
- Author
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Luo, Ai-Li, Gong, Yi-Jun, Yuan, Yuan, Zhang, Jing, Zhang, Cui-Cui, Zhang, Xiao-Bing, and Tan, Weihong
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FLUORESCENT probes , *HYDROGEN-ion concentration , *MERCURY , *METAL ions , *CHEMICAL research , *TOXICOLOGICAL chemistry - Abstract
Abstract: Development of fluorescent probes for Hg2+ has become a hot topic in modern chemical research due to its high toxicity. In this paper, we for the first time report the synthesis and application of a thioether spirocyclic rhodamine B derivative (TR) as an efficient fluorescent probe for Hg2+. TR was synthesized using a simple procedure under mild condition. By employing a thioether spirocycle instead of classic spirolactam as recognition unit, our proposed probe TR is acidity-insensitive, and exhibits a pH-independent and ultrasensitive response to Hg2+. The probe works well within a wide pH range from 3.5 to 11.5, and exhibits a 350-fold fluorescence enhancement upon 0.5equiv of Hg2+ triggered, with a detection limit of 2.5nM estimated for Hg2+. In virtue of the strong thiophilic characteristic of Hg2+, the response of the probe to Hg2+ is instantaneous and highly selective, which make it favorable for cellular Hg2+ imaging applications. It has been preliminarily used for highly sensitive monitoring of Hg2+ level in living cells with satisfying resolution, demonstrating its value of the practical applications in biological systems. [Copyright &y& Elsevier]
- Published
- 2013
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