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Fermented (F) and neutral (non-fermented) (N) beverages were developed to add value to cheese whey (CW) and secondary whey (SW) from goat (G) and sheep (S) cheese. All products were microbiologically safe. Regarding physical stability, sedimentation of 3–20% was observed, with higher values at the beginning of storage for SW beverages (P < 0.05) due to protein agglomeration (explained by the 22%-larger size of small particles, P < 0.05). F beverages exhibited pseudoplastic behavior (n < 0.8) and higher apparent viscosity (n app). Sensory evaluation showed that the ingredients/additives used masked the unpleasant taste of whey; however, N samples were preferred due to coffee and sweet/vanilla flavors (P < 0.05). The overall results indicated that treatment (N or F) overcame any differences observed in relation to animal source (G or S) or whey type (CW or SW) and that, for the fermented samples, the animal source was more important than the type of whey. [ABSTRACT FROM AUTHOR]

The pastoral area of internal Cilento (Campania, Southern Italy) claims many valuable cheeses and according to the oral tradition, when animal rennet was not available for goat cheeses, some wild plants were used for their curdling properties. The cheese market is in continuous evolution, looking for new cheeses but also linked to the territory and tradition. On the bases of an ethnobotanical investigation, the coagulating properties of water extracts of the wild species Teucrium chamaedrys L., Galium aparine L., Galium verum L., Silybum marianum (L.) Gaertn., Chelidonium majus L., and Urtica dioica L. were evaluated on goat milk by rheological test with a Formagraph instrument at two doses (60 μl vs. 50 μl per 10 mL goat milk) at 36 °C, and at lab-scale by micro cheesemaking test at 0.5 mL/100 mL goat milk. For all plant extracts, the best Milk Rheological Properties were found at the lowest dose of extract. In the lab-scale test, all plant extracts except C. majus showed interesting clotting behaviour. The results confirmed the pastoral tradition and opened to new perspectives to produce goat cheeses with no-animal rennet using these wild species, linked to the heritage of the geographical area. Further studies will be carried out on the chemical composition, besides the antioxidant capacity and polyphenol content, to attempt explaining the MRPs. • The request for dairy products made with non-animal rennet is increasing. • Pastoral tradition refers of wild plants used as alternative to lamb/kid rennet. • Plant extract's Milk Rheological Properties preliminarily characterize them as milk coagulant. [ABSTRACT FROM AUTHOR]

The addition of oilseeds and their cakes to the diets of lactating dairy goats is an alternative to supplemental feeding, which improves the lipid profile of goat cheeses. The objective of the present study was to evaluate the effect of a diet containing extruded linseed or pumpkin seed cake on the fatty acid profile of semi-hard cheese made from goat milk. The research was carried out with 28 French Alpine goats fed the following diets: 1—basal diet based on extruded soybean and soybean meal; 2—basal diet with 90 g/kg DM extruded linseed (ELS); and 3—basal diet with 160 g/kg DM pumpkin seed cake (PSC). Bulk milk from three separated milk tanks at three samplings was used for the manufacture of four traditional semi-hard cheeses from each milk tank at each sampling on the family farm. The ELS and PSC diets increased fat content in the cheese. The ELS feeding increased the proportion of C18:1 c9, C18:2 c9t11, and C18:3 n-3 in cheese and lowered C8:0, C6:0, and C16:0, while PSC resulted in the highest C18:2 n-6 proportions in the cheese. The health-promoting index was the highest in the cheese of ELS. The ELS had a contribution to higher nutritional and health quality of semi-hard traditional goat cheeses, thus representing a food with health-promoting properties.

The purpose of this paper is to investigate the effects of different freezing treatments on textural and sensorial characteristics of goat cheese in order to explore the possibility of storing for a longer period of time. Goat cheese samples were prepared from raw goat milk and treated under four different freezing methods:1) natural convection of air (- 200C); 2) deep freezing (-700C);3) immersion in eutectic solution of NaCl 23% and 4) cryogenic freezing using liquid nitrogen and compared with a control sample kept at 4-60C. After freezing, the samples were stored for 3 month at -18…-200C then they were thawed and stored for 14 days at 4…60C. The textural and sensory aspects were undertaken using 12 panelists. At the end, the samples were classified, depending on the score obtained from good to unsatisfactory.

The aim of this work was to study the influence of the ripening degree of natural goat cheese on texture, rheological and sensory properties of processed cheese products. Processed cheeses were formulated using goat cheeses with 10, 20, and 40 days of ripening. We obtained four different formulations by varying the proportions of these cheeses in each formulation. The variation in major α, β, and para-κ casein fractions, rheological properties, and the texture of samples were determined, and a sensorial evaluation was carried out. Cheeses from Formulation 2 (50% cheese ripened for 10 days, 25% cheese ripened for 20 days and 25% cheese ripened for 40 days) had greater values of α and β –caseins, which is related to a greater content of intact casein resulting from a cheese with short ripening time. Hardness, adhesiveness and complex modulus (G*) decreased as the degree of ripening of the natural cheese (raw material) increased. Formulation 2 presented a G* value similar to that of the commercial processed cow cheese and the greatest firmness. Formulation 2 presented the characteristics we aimed to obtain, described as spreadable, slightly acid and salty cheese.

Background: In Brazil, over the last few years there has been an increase in the production and consumption of goat cheeses. In addition, there was also a demand to create options to use the whey extracted during the production of cheeses. Whey can be used as an ingredient in the development of many products. Therefore, knowing its composition is a matter of utmost importance, considering that the reference methods of food analysis require time, trained labor and expensive reagents for its execution. Methods: Goat whey samples produced in winter and summer were submitted to proximate composition analysis (moisture, total solids, ashes, proteins, fat and carbohydrates by difference) using reference methods and near infrared spectroscopy (NIRS). The spectral data was preprocessed by baseline correction and the Savitzky-Golay derivative. The models were built using Partial Least Square Regression (PLSR) with raw and preprocessed data for each dependent variable (proximate composition parameter). Results: The average whey composition values obtained using the referenced methods were in accordance with the consulted literature. The composition did not differ significantly (p > 0.05) between the summer and winter whey samples. The PLSR models were made available using the following figures of merit: coefficients of determination of the calibration and prediction models (R2cal and R2pred, respectively) and the Root Mean Squared Error Calibration and Prediction (RMSEC and RMSEP, respectively). The best models used raw data for fat and protein determinations and the values obtained by NIRS for both parameters were consistent with their referenced methods. Consequently, NIRS can be used to determine fat and protein in goat whey. [ABSTRACT FROM AUTHOR]

Miracle fruit's potential benefit is encouraging as a powerful antioxidant and sweetness enhancer due to its novel ability to modify sour taste to sweet. However, further studies are needed to evaluate the practicality of different miracle fruit products. Thus, this study aimed to characterize the taste modification effects of different miracle fruit products on different sour foods. For this, 10 trained subjects (80% female, 50% white, Mean ± SE = 27.3 ± 3.9 years old) performed a Quantitative Descriptive Analysis using a Latin Square design with three replications. One session was composed of pre‐ and posttest for miracle fruit administration. Five food samples (green apple, goat cheese, lemonade, plain fat‐free yogurt, and cucumber pickle) and four miracle fruit products (three different brands of miracle fruit pill‐Y, G, M and one powder product‐P) were used and data were analyzed using analysis of variance and principal component analysis. The typical effects of miracle fruit, sweetness increase and sourness suppression were observed for all food samples albeit to different degrees. Y and M pills were most impactful while powder product was least, and goat cheese and yogurt had the most pronounced impact at increasing sweetness. Prior Miracle Fruit administration significantly decreased bitterness and increased creaminess in yogurt. However, it increased off‐flavor in lemonade and pickle. Results suggest that miracle fruit usage for increasing sweetness is effective but the degree of taste‐modifying effect differs according to the types of miracle fruit product and food samples applied. Practical Application: This research confirms miracle fruit's unique taste‐modifying abilities and demonstrates a high potential as a sweetness enhancer to benefit human health. The results can be used to optimize miracle fruit's application and can be applied by food industry and health care provider to develop clinical remedies or disease prevention strategies. [ABSTRACT FROM AUTHOR]

The aim of the present study was to evaluate the effect of dietary integration with dried licorice root on the chemical-nutritional qualities of goat milk and cheeses. The study was conducted for 60 d, during which 30 Saanen goats were divided into 2 groups: a control group (CG) that received a standard diet and an experimental group (LG+) whose diet was supplemented with licorice. At the end of the study, milk samples were collected to determine chemical-nutritional compositions and fatty acid (FA) profiles. Cheeses produced with CG and LG+ bulk milk were analyzed for chemical-physical parameters after 3 (T3) and 30 (T30) d of ripening. A different FA profile and a significant increase in protein and casein were observed in LG+ milk samples compared with CG milk. Regarding cheeses, an increase of proteins and fat was found in LG+ cheeses, which also were harder, more elastic, and more gummy than the CG samples after both 3 and 30 d of ripening. A different protein profile was detected in the 2 groups without significant variations in casein fractions (α S2 -casein and β-casein) during ripening. Moreover, greater oxidative stability was found in LG+ cheeses at both T3 and T30. Different families of volatile compounds were detected in T30 cheeses obtained from both groups. A significant reduction of octanoic acid and an increase in nonanal and ketones were found in LG+ T3 cheeses, whereas the LG+ T30 cheeses were characterized by a significant decrease of hexanoic acid an increase of 3-methyl-1-butanol and acetoin. We concluded that it is possible to assert that dietary integration with dried licorice root modified chemical and technological properties of goat cheeses, reducing lipid oxidation during ripening and inducing changes in texture that could improve consumer acceptability, although further studies are needed from this point of view. [ABSTRACT FROM AUTHOR]

Objective: This study aimed to evaluate the effect of dietary integration with extruded linseed (EL) on fatty acid (FA) and aromatic profile of goat cheese after 60 (T60) days of ripening. Methods: Thirty goats were divided in two groups. The control group (CG) was fed with conventional diet, whereas the experimental group (EL+) was fed with conventional diet supplemented with 10% of EL. Milk samples were collected on 30 and 60 days of trial to determinate chemical-nutritional composition and FA profile. At the end of experiment, six cheese-making sessions (3 for each group) were carried out using a pooled milk sample obtained from the 15 goats of each group. At 60 days of ripening, cheeses were analyzed for chemical-nutritional composition, FA and aromatic profile. Results: An increase in the milk production, protein, fat and lactose were evidenced in the EL+ goats. Conversely, a reduction of somatic cells was observed in the EL+ compared with the CG. However, no variation was observed for urea and casein levels content in milk samples, and no changes in protein and lipid content were found for cheeses in the two experimental groups. Dietary supplementation with EL modified the FA profile of milk. There was a decrease in saturated FAs and an increase in polyunsaturated FAs. Chemical composition of T60 cheese did not differ between the two groups but a different FA profile was observed. In T60 cheese obtained from EL+ milk, an increase in short-chain FA and a decrease in medium and long-chain FA were observed. The EL diet led to cheeses with butanoic acid 2 times higher compared to CG cheeses. Moreover, a greater presence of aldehyde compounds and alcohols were observed in the cheeses of experimental group. Conclusion: The present study pointed out that EL supplementation may improve the chemical and physical qualities of goat milk and cheeses. [ABSTRACT FROM AUTHOR]

The aim of this work was to study the influence of the ripening degree of natural goat cheese on texture, rheological and sensory properties of processed cheese products. Processed cheeses were formulated using goat cheeses with 10, 20, and 40 days of ripening. We obtained four different formulations by varying the proportions of these cheeses in each formulation. The variation in major α, β, and para-? casein fractions, rheological properties, and the texture of samples were determined, and a sensorial evaluation was carried out. Cheeses from Formulation 2 (50% cheese ripened for 10 days, 25% cheese ripened for 20 days and 25% cheese ripened for 40 days) had greater values of α and β -caseins, which is related to a greater content of intact casein resulting from a cheese with short ripening time. Hardness, adhesiveness and complex modulus (G*) decreased as the degree of ripening of the natural cheese (raw material) increased. Formulation 2 presented a G* value similar to that of the commercial processed cow cheese and the greatest firmness. Formulation 2 presented the characteristics we aimed to obtain, described as spreadable, slightly acid and salty cheese. [ABSTRACT FROM AUTHOR]

Robiola di Roccaverano is a Protected Designation of Origin soft cheese made with goat's milk, produced in Piedmont region (Italy). The peculiarities of this cheese are: i) the use of the raw milk, ii) the addition of a Natural Milk Starter, iii) the application of traditional techniques of production and iv) the localization of the dairies in rural area. All these aspects influence the microbial flora of final product and make interesting its investigation. Samples were collected at different moment of the cheese making process and during the different seasons. In this preliminary study, the safety and the hygiene parameters of the production were evaluated. Lactic acid bacteria, moulds and yeasts involved in cheese-making process were also enumerated. Pathogens were not found in all samples and the counts of coagulase positive staphylococci were within the standard of law. The enumeration of microorganisms of technological interest demonstrated that, nevertheless the artisanal manufacturing process applied, the dairy was able to standardize the final products. [ABSTRACT FROM AUTHOR]

The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk. The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography – Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry. A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples. A final product with recovery of 63–96% of oligosaccharides was obtained when compared with the original whey. These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition. [ABSTRACT FROM AUTHOR]

In this study the physicochemical, microbiological, and fatty acid compositions together with the specific esterase activities of semi-hard goat cheeses made from native strains as starter and adjunct cultures were evaluated and compared against those of manufactured using commercial culture cheeses. The physicochemical composition was similar among cheeses, while the lactic acid bacteria were the predominant microbiota in all samples. The highest specific esterase activities were detected in cheeses with native strains. The fatty acid profile was significantly affected by native strains during the ripening time (60 days) since the conjugated linoleic acid (CLA) level increased from 0.60 to 1.03 g 100 g-1 of fatty acids, whereas cheeses with commercial starter showed a CLA content of about 0.60 g of fatty acids. In cheeses with native strains, it was detected the highest desirable fatty acids, μ9-desaturase and CLA desaturase indexes and the lowest atherogenicity index. The native strains inoculated as starter and adjunct cultures, grew conveniently in the cheese, developed their full potential as reflected by the profile of the metabolites released during ripening and in the global sensory perception of cheeses, and contributed thus to the development of a healthier food. [ABSTRACT FROM AUTHOR]

Traces of antimicrobials in milk are of great concern for public health. The European Union has established maximum residue limits in milk; these, however, by themselves do not guarantee the absence of drug residues in milk and related products. Currently, very little information is available on the transfer of antibiotic residues from milk to other dairy products and their potential effect on food safety. This work evaluated the presence of antibiotic residues in pasteurized fluid milk and fresh cheeses from goat’s milk containing these veterinary drugs at legal safety levels (maximum residue limits) and assessed the safety margin of these dairy products for consumers. Eight antibiotics (amoxicillin, benzylpenicillin, cloxacillin, neomycin, erythromycin, ciprofloxacin, enrofloxacin, and oxytetracycline) were selected, and three batches of fresh cheese were made from pasteurized goat’s milk spiked with each of these drugs. Drug residues in milk and cheese samples were analyzed by liquid chromatography–tandem mass spectrometry. The safety margin of goat’s milk products was calculated taking into account different age groups (children, teenagers, and adults). Results showed that most antibiotics present in raw milk remained in pasteurized milk and were transferred to cheese to a high extent; retention was above 50% in most cases. The minimum safety margin in pasteurized milk was obtained for enrofloxacin, ciprofloxacin, and erythromycin for the children’s group. For fresh cheese, an elevated safety margin was obtained for all antibiotics and age groups considered. However, the large amounts of antibiotics retained in the cheese might contribute to the development and spread of antimicrobial resistance. Considering the differences in milk from different species and the great variety of cheeses, it would be advisable to continue the traceability study of antibiotics in order to increase the safety margin of dairy products. [ABSTRACT FROM AUTHOR]

A polymerase chain reaction (PCR) was applied to identify cows’ sheep's, goats’ and buffalo's milk and cheese's in Basra city by using specific primers targeting to identify the species of animals to detect the cheating in local and imported cheeses and in crude milk as a comparative point to detect accuracy of result come from amplified gene of cheeses. The present study aimed to develop a rapid PCR-based method for the detection of cows’, sheep's, goats’ and buffalo’ cheese. The current study revealed that there was no cheating in imported cheeses, whereas, reported some cheating in the local cheeses compared with the crude milk as a standard to limited the accuracy of result. [ABSTRACT FROM AUTHOR]

Semi-hard, hard, and extra-hard goats' cheeses were analysed for water mobility by nuclear magnetic resonance relaxometry (NMR) and thermal properties by differential scanning calorimetry (DSC). In extra-hard cheese, the amount of bulk water as a proportion of the total water was the lowest, which indicated that the water was strongly entrapped in the proteolipidic network and bound by the water-soluble substances arising after increased proteolysis. The bulk water fraction is mainly a component of water-in-fat emulsions. The increase in H+ ion concentration limits the mobility of bound water protons. Both the peak temperature and enthalpy progressively decreased with the lowering of moisture. A strong correlation was found between the NMR parameter T 1 (spin–lattice relaxation time) and the DSC freezable water content. [ABSTRACT FROM AUTHOR]

Eight different commercial enzyme‐modified cheeses (EMCs) were analysed, and the distinctive/common features of the products and production methods were investigated. Results showed that the total free fatty acid contents of EMC samples were 10 to 100 times higher than the values reported for the related cheese varieties. A total of 37 volatile compounds were identified, and acids were found as the most dominant group in all EMC samples. While furan compounds and 2‐acetylpyrrole were most intensively detected in the goat cheese EMC, methyl ketones were found in the highest amounts in Blue cheese EMC. [ABSTRACT FROM AUTHOR]

Reports of β-lactam-resistant Staphylococcus aureus in artisanal goat cheese are increasing, and this phenomenon is relevant to public health. The objective of the present study was to determine the prevalence of S. aureus strains carrying the blaZ and mecA resistance genes, as well as the genes encoding the staphylococcal enterotoxins SEA, SEB, SEC, SED, SEE, and TSST-1 in artisanal coalho cheese made from goat milk produced in northeastern Brazil. We used biochemical and molecular tests to characterize 54 S. aureus isolates found in artisanal coalho cheese collected from commercial establishments producing animal products in 11 municipalities of Pernambuco State, Brazil. A PCR analysis revealed that 42.6% (23/54) of the isolates were positive for the blaZ gene, and 7.4% (4/54) were resistant to methicillin by phenotypic testing. We did not detect mecA or any genes encoding enterotoxins. The presence of S. aureus carriers of the blaZ gene and the identification of methicillin-resistant S. aureus strains are of concern for the health of consumers of this type of cheese. [ABSTRACT FROM AUTHOR]

Dairy goat production continues to be a socially, economically and culturally important part of the livestock industry in North, Central and South America and the Caribbean islands. Goat milk, cheese and other dairy products offer consumers food products with nutritional, health and environmental benefits. In North America, Mexico produces the greatest volume of goat milk, but most is for family or local consumption that is typical of a mixed farming system adopted by subsistence farmers in dry areas. The United States is not yet a large global goat milk producer, but the sector has expanded rapidly, with dairy goat numbers doubling between 1997 and 2012. The number of dairy goats has also increased dramatically in Canada. Commercial farms are increasingly important, driven by rising demand for good quality and locally sourced goat cheese. In South America, Brazil has the most developed dairy goat industry that includes government assistance to small-scale producers and low-income households. As of 2017, FAO identified Haiti, Peru, Jamaica, and Bolivia as having important goat milk production in the Western Hemisphere. For subsistence goat producers in the Americas on marginal land without prior history of chemical usage, organic dairy goat production can be a viable alternative for income generation, with sufficient transportation, sanitation and marketing initiatives. Production efficiency, greenhouse gas emission, waste disposal, and animal welfare are important challenges for dairy goat producers in the Americas. [ABSTRACT FROM AUTHOR]

The global goat population continues to grow and is now over one billion. The number of goats raised primarily for milk production is also growing, due to expanding demand. Most of the world dairy goat production and consumption is in Asia, but a global view of the dairy goat sector reveals important lessons about building successful modern dairy goat industries. The most organized market for goat milk is found in Europe, especially in France. The European goat sector is specialized for milk production, mostly for industrial cheesemaking, while also supporting traditional on-farm manufacturing. Government involvement is significant in sanitary regulation, research, extension, support for local producer organizations, and markets, and ensures safety and quality. Nonetheless, producers are still vulnerable to market fluctuations. New dairy goat industries are developing in countries without a long goat milk tradition, such as China, the United States, and New Zealand, due to rising consumer demand, strong prices, and climate change. The mix of policies, management and markets varies widely, but regardless of the country, the dairy goat sector thrives when producers have access to markets, and the tools and skills to sustainably manage their livestock and natural resources. These are most readily achieved through strong and inclusive producer organizations, access to technical services, and policies that enable the poor and marginalized groups to benefit from increasing demand. [ABSTRACT FROM AUTHOR]

Summary: Linear regression models were applied to predict the effect of the breed and technological factors on the sensory characteristic of cheeses. Seventy goat cheeses from forty local producers were analysed. They represented the totality of the Andalusian cheese‐making sector (different goat breeds, type of coagulant, ripening time and heat treatment of milk). Sensory attributes of cheeses were influenced by all the factors studied. Ripening affects all sensory attributes while the breed and the heat treatment are the most influential factors on the flavour and the type of coagulant on the texture attributes of these cheeses. Linear regression models were found to be suitable for studying which factors had an independent influence on the sensory attributes of these cheeses. This work could help researchers and producers to a better sensory characterisation of their products. [ABSTRACT FROM AUTHOR]

Copyright of Lucrari Stiintifice, Universitatea de Stiinte Agricole Si Medicina Veterinara Ion Ionescu de la Brad Iasi, Seria Horticultura is the property of University of Agricultural Sciences and Veterinary Medicine (Editura Ion Ionescu de la Brad) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this study, a collection of 298 wild strains of lactic acid bacteria (LAB) isolated from raw goat milk cheeses, belonging to 8 genera and 24 species, was investigated for their ability to generate volatile compounds. Sensory evaluation showed that goat milk cultures of Leuconostoc and Lactococcus strains reached the highest scores for dairy odour attributes. Fifty six LAB strains with the highest sensory evaluation scores (one per each ten, or fraction, of those belonging to the same species) were selected for gas chromatography–mass spectrometry (GC–MS) analysis. A total of 34 volatile compounds (five carboxylic acids, eleven alcohols, six aldehydes, six ketones, one ester, and five miscellaneous compounds) were detected in lactic curds made with each one of 56 selected LAB strains. The number of volatile compounds in individual curds varied from 15 to 33 while the total abundance of volatile compounds in individual curds ranged from 1.98- to 17.50-fold the total abundance in uninoculated curd. Major differences in volatile generation were related to amino acid catabolism. • Leuconostoc and Lactococcus obtained the highest scores for dairy odour attributes. • Up to 33 volatile compounds detected in lactic curds made with individual strains • Volatile production differences among members of the same genus and species • Research on volatile production by dairy-related bacteria not previously studied [ABSTRACT FROM AUTHOR]

To produce a wide variety of cheeses, it is necessary to control the ripening process. To do that, artisanal goat cheeses were ripened to evaluate the effects of temperature (10 and 14°C) and relative humidity (RH; 88 and 98%) on (1) 16 physicochemical characteristics throughout ripening and (2) 19 sensory characteristics at the end of ripening (d 12). Whatever the ripening time, the physicochemical characteristics were strongly dependent on the daily productions, which affected the sensory perception of the cheeses. Both physicochemical and sensory characteristics were strongly reliant on RH, whereas only a few of the characteristics were influenced by temperature changes. On d 12, whatever the ripening temperature, an RH increase from 88% to 98% modified many cheese characteristics (core pH, lactate consumption, underrind thickening, dry matter content, and hardness). As a result of these physicochemical properties, changes in perception were observed: the cheeses ripened under 88% RH were dry and hard compared with those ripened under 98% RH. An RH of 98% led to an acceleration of the ripening process, inducing a slightly ammonia and milky flavor and a sticky and creamy texture in the mouth. However, cheeses ripened under 14°C and 98% RH were also indicative of overripened cheeses: a temperature of 14°C induced an acceleration of the ripening process due to physicochemical modifications compared with a temperature of 10°C. Nevertheless, when the cheeses on d 0 were still very humid and soft, those ripened under 98% RH collapsed and were overripened with a liquid underrind. This study provides a means for achieving a better and more rational control of the ripening process in artisanal lactic goat cheeses. [ABSTRACT FROM AUTHOR]

Background: Monitoring the acidity of cheese is an important control mechanism in various stages of manufacture, including aging. Acid development in cheesemaking is essential to cheese flavor, texture, and safety. Objective: The aim of the work was to develop and validate calibration models by using NIR spectroscopy, which allows for the monitoring of changes in cheese acidity (pH and titration acidity) during cheese ripening. Methods: Cheeses were analyzed by an FT-NIR spectrometer. Each of the samples was analyzed three times, and for calibration, an average spectrum was used. A partial least-squares regression was used to develop calibration models. The constructed calibration models were validating by full cross-validation. Results: Calibration models were created with a high correlation coefficient for the following cheese pH levels: blue cheese (0.966), Olomouc curd read smear cheese (0.984), and fresh goat cheese (0.980). Results of the calibration of titratable acidity are functional for fresh goat cheese (0.953) and mozzarella (0.999). Conclusions: The results of these new calibration methods showed the possibility of NIR technology for the fast determination of pH and titratable acidity. Highlights: Detection of cheese acidity using FT-NIR spectrometry enables rapid evaluation of the process of lactic acidification in particular cheese technological operations, including the maturing of cheeses. [ABSTRACT FROM AUTHOR]

In the main purpose of this study is to determine chemical and microbiological properties of cheese produced from goat milk, and to identify the antioxidant properties of peptides during proteolysis. For this aim; Cheese samples were produced from goat raw milk by traditional method. Chemical and microbiological properties of cheeses were determined 0th, 3rd, 6th, 9th months of ripening. To determine the level of proteolysis during ripening period of the cheeses, total protein, water soluble nitrogen, 12 % TCA soluble nitrogen 5% PTA soluble nitrogen, and total free amino acid analysis were carried out. The peptide profile of the cheese samples was determined by RP-HPLC during ripening period. Peptide fractions were collected and lyophilized. Antioxidant act ivity of the lyophilized fractions was determined. Antioxidant activity of goat cheese were found 29.34-55.12 mM Trolox/g. Antioxidant activity were found in the F3, F4, F5 fractions in 6 month 112.13mM Trolox/g, 841.04 mM Trolox/g, 416.95mM Troloks/g, and in the F3, F4, F6 fractions of 9.month 895.44 mM Trolox/g, 841.14 mM Trolox/g, 595.88 mM Trolox/g respectively. [ABSTRACT FROM AUTHOR]

The paper deals with microbiological parameters of goat cheeses produced on two farms during several years. The first farm used conventional farming method with about 130 dairy goats and the second one applied organic farming method with approximately 500 dairy goats. In samples of fresh goat cheese taken in four years, there were determined the following groups of microorganisms: total count of microorganisms, lactic acid bacteria, coliform bacteria, psychrotrophic microorganisms, enterococci and micromycetes. The comparison of the average numbers of individual groups of microorganisms in cheeses showed a statistically significant difference between the farms. Microbiological quality was found worse at cheeses manufactured at the farm using organic farming method, compared with cheeses from the conventional farm. Higher numbers of coliforms, psychrotrophic microorganisms, enterococci and fungi were more frequently detected in cheeses from the organic farm. Worse microbiological quality of the cheese was also reflected in sensory properties, especially the smell, colour and consistency which was evident when preparing individual samples. [ABSTRACT FROM AUTHOR]

Abstract The present study focuses on the effect of gas permeability on dry matter, colour, inner- and outer texture, and sensory properties of foil-ripened 0.5 kg semi-hard goat cheese during 3 weeks of ripening (13 °C; 88% relative humidity). The characteristics of the packaging foils applied were made of different materials and varied in thickness, gas permeability (CO 2 , O 2) and water vapour permeability. All ripening foils decreased the inner lightness of samples after 3 weeks; however, yellowness was influenced by the gas permeation. The equalisation of outer- and inner hardness was measurable after 1 week of ripening; gas permeability contributed to the speeding up of this process. The difference between inner and outer elasticity declined until 1.5 weeks. Sensory evaluation revealed that the foil with permeabilities (cm3 m−2 24 h−1 1 atm−1) for O 2 , CO 2 and water vapour of 400, 1250 and 15, respectively, contributed to best appearance, taste and texture. [ABSTRACT FROM AUTHOR]

Aim: The research was conducted to determine the effect of temperature and storage duration on the physicochemical, lipolytic, microbiological, and proteolytic characteristics of goat cheese made using Lactobacillus plantarum TW14 and Lactobacillus rhamnosus TW2 bacteria. Materials and Methods: The cheese was stored at 4°C and -20°C for 0, 15, 30, 45, and 60 days. Observations were made on its physicochemical, lipolysis, and microbiological characteristics. The proteolysis pattern was measured with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results: The protein, fat, ash and total solids levels of cold-stored cheese were higher than the frozen-stored one. The frozen-stored cheese's free fatty acids (FFA) and acid degree value (ADV) levels are lower than those of the cold-stored cheese as indicated by the partial lipolysis event. The total yeast in the frozen-stored cheese is lower than that in the frozenstored cheese. Finally, the electrophoresis profile indicates that proteolysis of the frozen-stored cheese is formed since there have been detected αs1-casein, αs2-casein, β-casein, and κ-casein in the casein breakdown during the 60-day storage. Conclusion: The physicochemical characteristics of cold-stored cheese are better than the cheese stored at frozen temperature. However, frozen-stored cheese produces lower FFA and ADV than cold-stored cheese and lipolysis occurs only partially. [ABSTRACT FROM AUTHOR]

HiSorb and solid-phase microextraction (SPME), two environmentally friendly micro-extraction techniques based on the same fundamental principles, were evaluated for their extraction efficiency of volatile organic compounds (VOCs) from goat cheese whey wastewater. For this purpose, a sample preparation method based on the headspace-HiSorb technique was developed and evaluated for its efficiency in terms of the amount of extracted compounds and reproducibility of results. Thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) and GC/MS analytical methods were used to perform the wastewater analysis, respectively. The experimental parameters of HiSorb were evaluated in terms of probe coating, extraction time, stirring speed, sample volume, extraction temperature and salt addition. Under optimal extraction conditions, it was observed that the use of the divinylbenzene/carbon wide range/polydimethylsiloxane (DVB/CWR/PDMS) triple coating for HiSorb and DVB/Carboxen (CAR)/PDMS for SPME, was best suited to extract a broader range of VOCs with higher peak intensities. A total of 34 VOCs were extracted and determined with the DVB/CWR/PDMS HiSorb probe, while only 23 VOCs were determined with the conventional DVB/CAR/PDMS SPME fiber. The DVB/CWR/PDMS HiSorb probe has a higher adsorbent capacity which results in a higher sensitivity for VOCs compared to the DVB/CAR/PDMS SPME fiber. Furthermore, the HiSorb technique exhibits better reproducibility, as indicated by the lower relative standard deviation (RSD) of 3.7% compared to 7.1% for SPME. Therefore, the HiSorb technique is an effective method for detecting VOCs in complex matrices, such as wastewater. [Display omitted] • The aroma profile of goat cheese whey wastewater effluent was demonstrated. • HiSorb and SPME sample preparation micro-extraction techniques were investigated. • Coating material, extraction time, temperature and salting effect were optimized. • Three-phase coating material was better for the extraction of respective analytes. • HiSorb sorptive is suitable for the extraction of goat cheese wastewater volatiles. [ABSTRACT FROM AUTHOR]

The valorization of sheep and goat cheese whey are important from economic, social and environmental perspectives. However, their low microbiological and physical stabilities are major challenges, especially for artisanal productions. In this study, the effectiveness of nisin addition, bioprotective culture (Lacticaseibacillus casei) inoculation and direct acidification (pH 2.5–4.5) was evaluated to improve the stability of pasteurized (75 °C/5 min) sheep and goat cheese whey. All strategies were effective in controlling the native microbial population, with minimal or no change in the pH or acidity during 28 days/7 °C, whereas samples only pasteurized reached counts of up to 8 log CFU/mL. Phase separation occurred in all samples, but at different rates and intensities. Acidification at pH 3.5 and 4.5 resulted in higher creaming rates (up to 30%), even without change in fat globule size, and sedimentation (up to 6%), due to protein particle agglomeration - that increased D [3,2] values by up to 27% and reduced the specific surface area by around 20%. Conversely, acidification at pH 2.5 or the addition of nisin or L. casei resulted in samples with smaller particle size and better stability to creaming and sedimentation. The results indicated that nisin addition or L. casei inoculation are possible strategies to improve the shelf life of sheep and goat pasteurized whey, which can be used even by artisanal producers, and thus contributing for their valorization. • We develop strategies for sheep (SCW) and goat whey (GCW) valorization. • Nisin, L. casei and direct acidification (pH 2.5–4.5) inhibited SCW/GCW contaminants. • Acidification increased particle size and caused cream separation and sedimentation. • Nisin and L. casei were the best strategies to stabilize SCW and GCW. • The developed solutions can be directly applied in artisanal production. [ABSTRACT FROM AUTHOR]

A procedure to produce a hard cheese made from goat milk set to approach Manchego cheese was developed. The effects on cheese characteristics of two scalding temperatures, 38 °C and 40 °C, during cheesemaking were evaluated in an experimental design with three replicate blocks. The cheese was analysed for volatiles, organic acids, proteolysis (as measured by capillary electrophoresis) and sensory properties. After 18 weeks of ripening, sensory descriptive analysis showed that the cheeses had a high total flavour and odour intensity, and a low degree of oxidised flavour. A consumer acceptability test showed that the cheese was liked. Cheese scalded at 40 °C obtained higher concentrations of volatiles and was described as more mature, umami, sweet and sour compared with cheese scalded at 38 °C, which had the highest concentration of organic acids and was described to be slightly more oxidised, bitter, and acidic. [ABSTRACT FROM AUTHOR]

Cheese production has increased more than 3.5-fold since 1960, while animal rennet production has declined, thus other proteolytic enzymes have been developed. Cheese quality depend on many factors, some of them are linked to milk composition (genetic aspects: species, breed and animal, lactation stage and number, type of kidding, animal feeding and health, offspring season, etc.) and other due to cheesemaking aspects and storage processing (raw or pasteurized milk, coagulation enzymes and coagulation parameters (temperature and time), moulding, drainage, salting, ripening, smoking, etc.). All these factors interact and only under experimental conditions it is possible to analyse the effect of any particular feature by itself. Between all these factors, milk coagulation process is one of the most important steps for the cheese final characteristics. The differences in protein matrix degradation due the origin of coagulant agents affect cheese texture, odour, flavour and taste and consequently consumer's preference. This last assertion is especially important for the economic choices of the dairy firm. In the past, cheesemakers used animal rennet for curdling milk; it was produced by macerating the stomachs obtained from suckling ruminants according to the local uses, although plant coagulant was used in some typical specific cheesemaking processes. Nowadays, several types of coagulant are available, with different origin and variability in proteases for milk coagulation: animal origin rennet and vegetable coagulants than can be both artisan or commercial, microbial proteases and recombinant from genetically modified microorganism with cDNA for chymosin. In this paper the state of the art of these enzymes used in goat´s cheese industry is presented, also focused on artisan cheeses. Coagulant effect in physical-chemical and sensory profile of cheeses is analysed, also under an ethical and religious point of view. Finally, the importance of the market target in producing new cheese with vegetable coagulant is met, with an overview on the trends on cheese labelling issue. • The market for dairy products made with non-animal rennet is increasing. • Several types of coagulant are available, different for origin and proteases. • Effect of coagulant on cheese quality must be taken into account when planning dairy products with non-animal rennet. [ABSTRACT FROM AUTHOR]