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While roots and leaves have evolved independently in lycophytes, ferns and seed plants, there is still confusion regarding the morphological evolution of ferns, especially in whisk ferns, which lack true leaves and roots and instead only exhibit leaf-like appendages and absorptive rhizoids. In this study, analyses of comparative transcriptomics on positively selected genes were performed to provide insights into the adaptive evolution of whisk fern morphologies. Significantly clustered gene families specific to whisk ferns were mainly enriched in Gene Ontology (GO) terms “binding proteins” and “transmembrane transporter activity”, and positive selection was detected in genes involved in transmembrane transporter activities and stress response (e.g., sodium/hydrogen exchanger and heat shock proteins), which could be related to the adaptive evolution of tolerance to epiphytic environments. The analysis of TF/TR gene family sizes indicated that some rapidly evolving gene families (e.g., the GRF and the MADS-MIKC families) related to the development of morphological organs were commonly reduced in whisk ferns and ophioglossoid ferns. Furthermore, the WUS homeobox-containing (WOX) gene family and the knotted1-like homeobox (KNOX) gene family, both associated with root and leaf development, were phylogenetically conserved in whisk ferns and ophioglossoid ferns. In general, our results suggested that adaptive evolution to epiphytic environments might have occurred in whisk ferns. We propose that the simplified and reduced leaf and root system in whisk ferns is the result of reduction from the common ancestor of whisk ferns and ophioglossoid ferns, rather than an independent origin.

Despite considerable progress in recent decades in dissecting the genetic causes of natural morphological variation, there is limited understanding of how variation within species ultimately contributes to species differences. We have studied patterning of the non‐sensory hairs, commonly known as "trichomes," on the dorsal cuticle of first‐instar larvae of Drosophila. Most Drosophila species produce a dense lawn of dorsal trichomes, but a subset of these trichomes were lost in D. sechellia and D. ezoana due entirely to regulatory evolution of the shavenbaby (svb) gene. Here, we describe intraspecific variation in dorsal trichome patterns of first‐instar larvae of D. virilis that is similar to the trichome pattern variation identified previously between species. We found that a single large effect QTL, which includes svb, explains most of the trichome number difference between two D. virilis strains and that svb expression correlates with the trichome difference between strains. This QTL does not explain the entire difference between strains, implying that additional loci contribute to variation in trichome numbers. Thus, the genetic architecture of intraspecific variation exhibits similarities and differences with interspecific variation that may reflect differences in long‐term and short‐term evolutionary processes. [ABSTRACT FROM AUTHOR]

Many species possess warning colourations that signal unprofitability to predators. Warning colourations are also thought to provide prey with a 'predator-free space' and promote niche expansion. However, how such strategies release a species from environmental constraints and facilitate niche expansion is not clearly understood. Fossoriality in reptiles imposes several morphological limits on head and body size to facilitate burrowing underground, but many fossorial snakes live close to the surface and occasionally move above ground, exposing them to predators. In such cases, evolving antipredator defences that reduce predation on the surface could potentially relax the morphological constraints associated with fossoriality and promote morphological diversification. Fossorial uropeltid snakes possess varying degrees of conspicuous warning colourations that reduce avian predation when active above ground. We predicted that species with more conspicuous colourations will exhibit more robust body forms and show faster rates of morphological evolution because constraints imposed by fossoriality are relaxed. Using a comparative phylogenetic approach on the genus Uropeltis , we show that more conspicuous species tend to have more robust morphologies and have faster rates of head-shape evolution. Overall, we find that the evolution of warning colourations in Uropeltis can facilitate niche expansion by influencing rates of morphological diversification. [ABSTRACT FROM AUTHOR]

Morphologically similar species, that is cryptic species, may be similar or quasi-similar owing to the deceleration of morphological evolution and stasis. While the factors underlying the deceleration of morphological evolution or stasis in cryptic species remain unknown, decades of research in the field of paleontology on punctuated equilibrium have originated clear hypotheses. Species are expected to remain morphologically identical in scenarios of shared genetic variation, such as hybridization and incomplete lineage sorting, or in scenarios where bottlenecks reduce genetic variation and constrain the evolution of morphology. Here, focusing on three morphologically similar Stygocapitella species, we employ a whole-genome amplification method (WGA) coupled with double-digestion restriction-site associated DNA sequencing (ddRAD) to reconstruct the evolutionary history of the species complex. We explore population structure, use population-level statistics to determine the degree of connectivity between populations and species, and determine the most likely demographic scenarios which generally reject for recent hybridization. We find that the combination of WGA and ddRAD allowed us to obtain genomic-level data from microscopic eukaryotes (-1 millimetre) opening up opportunities for those working with population genomics and phylogenomics in such taxa. The three species share genetic variance, likely from incomplete lineage sorting and ancient admixture. We speculate that the degree of shared variation might underlie morphological similarity in the Atlantic species complex. [ABSTRACT FROM AUTHOR]

The concept of developmental constraints has been central to understand the role of development in morphological evolution. Developmental constraints are classically defined as biases imposed by development on the distribution of morphological variation. This opinion article argues that the concepts of developmental constraints and developmental biases do not accurately represent the role of development in evolution. The concept of developmental constraints was coined to oppose the view that natural selection is all-capable and to highlight the importance of development for understanding evolution. In the modern synthesis, natural selection was seen as the main factor determining the direction of morphological evolution. For that to be the case, morphological variation needs to be isotropic (i.e. equally possible in all directions). The proponents of the developmental constraint concept argued that development makes that some morphological variation is more likely than other (i.e. variation is not isotropic), and that, thus, development constraints evolution by precluding natural selection from being all-capable. This article adds to the idea that development is not compatible with the isotropic expectation by arguing that, in fact, it could not be otherwise: there is no actual reason to expect that development could lead to isotropic morphological variation. It is then argued that, since the isotropic expectation is untenable, the role of development in evolution should not be understood as a departure from such an expectation. The role of development in evolution should be described in an exclusively positive way, as the process determining which directions of morphological variation are possible, instead of negatively, as a process precluding the existence of morphological variation we have no actual reason to expect. This article discusses that this change of perspective is not a mere question of semantics: it leads to a different interpretation of the studies on developmental constraints and to a different research program in evolution and development. This program does not ask whether development constrains evolution. Instead it asks questions such as, for example, how different types of development lead to different types of morphological variation and, together with natural selection, determine the directions in which different lineages evolve. [ABSTRACT FROM AUTHOR]

Bare and Fe doped vanadium sulfide nanorods was synthesized by employing sol-gel method. The obtained (011) plane revealed and confirmed the orthorhombic phase of vanadium sulfide (VS). Fe doped vanadium sulfide anemone like nanorods structure were demonstrated by morphological evolution employing SEM studies. FTIR studies confirmed the vibrational frequencies of the prepared samples and identify the functional groups. Raman technique is also used to observe the vibrational modes of molecules and PL revealed the prepared samples optical-electronic nature. Additionally, electrochemical studies such as cyclic voltammetry (CV), linear sweep voltammetry (LSV), electrochemical impedance spectroscopy (EIS) and chronoampherometry (CA) was recorded for determination of specific capacitance, current density and stability. High doped vanadium sulfide has elevated 1372 F/g specific capacitance at 385 mA/g current density attained from CV and LSV curves at 10 mV/s and it has proven the excellent OER activity. Image 1 • An excellent electrochemical water oxidation was demonstrated. • Fe doped VS exhibited outstanding 385 mA/g current density. • Fe doped VS exhibited very low 154 mV overpotential. [ABSTRACT FROM AUTHOR]

The kinetic skull is a key innovation that allowed snakes to capture, manipulate, and swallow prey exclusively using their heads using the coordinated movement of eight bones. Despite these unique feeding behaviors, patterns of evolutionary integration and modularity within the feeding bones of snakes in a phylogenetic framework have yet to be addressed. Here, we use a dataset of 60 μCT‐scanned skulls and high‐density geometric morphometric methods to address the origin and patterns of variation and integration in the feeding bones of aquatic‐foraging snakes. By comparing alternate superimposition protocols allowing us to analyze the entire kinetic feeding system simultaneously, we find that the feeding bones are highly integrated, driven predominantly by functional selective pressures. The most supported pattern of modularity contains four modules, each associated with distinct functional roles: the mandible, the palatopterygoid arch, the maxilla, and the suspensorium. Further, the morphological disparity of each bone is not linked to its magnitude of integration, indicating that integration within the feeding system does not strongly constrain morphological evolution, and that adequate biomechanical solutions to a wide range of feeding ecologies and behaviors are readily evolvable within the constraint due to integration in the snake feeding system. [ABSTRACT FROM AUTHOR]

Han, Z.; Xie, H.; Li, H.; Li, W.; Wen, X., and Xie, M., 2021. Morphological evolution of the Lingding Channel in the Pearl River Estuary over the last decades. Journal of Coastal Research, 37(1), 104–112. Coconut Creek (Florida), ISSN 0749-0208. Dominated by the combined influence of fluvial and marine processes as well as human activities, estuarine regions are facing serious challenges, especially Lingding Bay in the Pearl River Estuary (PRE). The Lingding Channel is the main estuarine channel in Lingding Bay, and it connects to the outer sea, so its morphological evolution is very critical for navigation and estuarine management. Underwater topography data and remote sensing images of Lingding Bay in the PRE were employed to examine the morphological evolution of the Lingding Channel over the last decades (1965–2016). The results show that the West Shoal in Lingding Bay has extended eastward through a silting process caused by land reclamation and sediment discharge from the Pearl River; consequently, the Lingding Channel has become narrow. Additionally, the Lingding Channel has become deep because of channel regulation activities. In the last 10 years, large-scale sand-dredging activities at the Fanshi Shoal have deepened the shallow shoal near Lingding Channel, and many large and deep holes have formed with irregular shapes. In summary, drastic changes have occurred in the landforms near the Lingding Channel, which will likely influence the future stability of the channel. This represents a new and concerning challenge. Furthermore, sand-dredging activities could also produce large amounts of turbid water that could be transported into the Lingding Channel and increase the siltation rate. [ABSTRACT FROM AUTHOR]

The Neotropical region is highly diverse in fresh water teleost fishes and extends through vast regions of South America. The genus Astyanax comprises small fish found in the tributaries and ditches of the great Brazilian basins, such as the Paraná and São Francisco Rivers. This group has been ostensibly studied from a chromosomal and molecular point of view but is still involved in problems of phylogenetic location, justifying its position incertae sedis, while having a wide diversity of forms and habitats. Our study counted on the analysis of body shape variations in seven species of the genus Astyanax and in the bimaculatus, fasciatus, and scabripinnis complexes using geometric morphometrics. We obtained images of individuals from different localities in the Paraná and São Francisco River basins, delineated two-dimensional anatomical landmarks that we then subjected to the generalized Procrustes analysis, and subsequent ordination by principal component analysis and canonical variate analysis. The results showed variation between two large groups, related mainly to variations in body height, indicating the probable existence of different evolutionarily significant units morphologically shaped by the pressure of the environment with what may be incipient speciation. [ABSTRACT FROM AUTHOR]

Darwin's finches are a classic example of adaptive radiation, a process by which multiple ecologically distinct species rapidly evolve from a single ancestor. Such evolutionary diversification is typically explained by adaptation to new ecological opportunities. However, the ecological diversification of Darwin's finches following their dispersal to Galápagos was not matched on the same archipelago by other lineages of colonizing land birds, which diversified very little in terms of both species number and morphology. To better understand the causes underlying the extraordinary variation in Darwin's finches, we analyze the evolutionary dynamics of speciation and trait diversification in Thraupidae, including Coerebinae (Darwin's finches and relatives) and, their closely related clade, Sporophilinae. For all traits, we observe an early pulse of speciation and morphological diversification followed by prolonged periods of slower steady‐state rates of change. The primary exception is the apparent recent increase in diversification rate in Darwin's finches coupled with highly variable beak morphology, a potential key factor explaining this adaptive radiation. Our observations illustrate how the exploitation of ecological opportunity by contrasting means can produce clades with similarly high diversification rate yet strikingly different degrees of ecological and morphological differentiation. [ABSTRACT FROM AUTHOR]

The rodent family Echimyidae (spiny rats, hutias and coypu) is notable for its high phylogenetic and ecological diversity, encompassing ~100 living species with body mass ranging from 70 to 4500 g, including arboreal, epigean (non-arboreal or scansorial), fossorial and semi-aquatic taxa. In view of this diversity, it was hypothesized that echimyid morphological variation in the pelvis and femur should reflect: (1) allometric association with body mass; (2) morphofunctional specializations for the different locomotor habits; and (3) phylogenetic history. To test these propositions, we examined 30 echimyid species, in addition to eight species of two other octodontoid families, Abrocomidae and Octodontidae. Pelvic and femoral variation was assessed with linear morphometry, using bivariate and multivariate statistical methods, part of which was phylogenetically informed. Approximately 80% of the total variation among echimyids was explained by body mass, and some univariate measurements were found potentially to be effective as body mass estimators after simple allometric procedures, notably in the pelvis. Even considering the significant phylogenetic signal, variation in shape was largely structured by locomotor habits, mainly in the pelvis, suggesting that the echimyid hindlimb diversification was driven, in part, by selective pressures related to locomotor habits. Finally, echimyid femoral disparity was considerably greater than in other octodontoids, contrasting with their relatively modest cranial variation. Thus, this study suggests that hindlimb diversity constitutes a key factor for the exceptional echimyid ecological and phyletic diversification. [ABSTRACT FROM AUTHOR]

The Tetonius-Pseudotetonius (T-P) transition is an often-cited example of phyletic gradualism, but rates of evolution and the roles of neutral and adaptive processes across this lineage remain unclear. Linking Tetonius and Pseudotetonius, two omomyid primates, are a series of stratigraphic and morphologic intermediates revealing trends suggestive of a functional and developmental reorganization of the dentition. Notable changes involved the P3, which reduced size and became indistinguishable from the canine and I2, and the P4, which became a robust tall-cusped tooth comparable to a molar. We test two hypotheses: (1) neutral evolution can explain the observed phenotypic differences in the lineage, and (2) P4 lost developmental association with P3 and became integrated with the molars. First, we calculate the rate of evolutionary differentiation, based on the ratio between inter- and intra-species variation in length and width of P3, P4, M1, and M2 teeth, between lineage segments and over the entire lineage. We test the second hypothesis by comparing bivariate correlations between teeth within individual lineage segments. As the lineage evolved, correlations between P3 and the molars diminished, whereas correlations between P4 and the molars increased. We found evidence of varying degrees of stabilizing selection in the lengths and widths of all cheek teeth and evidence of neutral evolution in the width of P4. These results support a trend towards P4 becoming integrated into the molar morphogenetic field, and demonstrates that morphological rates of evolution, and consequently the degree of selective pressures, vary through time and between teeth. [ABSTRACT FROM AUTHOR]

The evolution of open-channel flow and morphology can be simulated by one-dimensional (ID) mathematical models. These models are typically solved by numerical or analytical methods. Because the behavior of variables can be explained by explicit mathematical determinations, compared to numerical solutions, analytical solutions provide fundamental and physical insights into flow and sediment transport mechanisms. The singular perturbation technique derives a hierarchical equation of waves and describes the evolutionary nature of disturbances in hyperbolic systems. The wave hierarchy consists of dynamic, diffusion, and kinetic waves. These three types of waves interact with each other in the process of propagation. Moreover, the Laplace transform is implemented to transform partial differential equations into ordinary differential equations. Analytical expressions in the wave front are subtracted by the approximation of kinetic and diffusion models. Moreover, an analytical solution consists of a linear combination of the kinetic wave front and the diffusion wave front expressions, pursuing to describe the physical mechanism of motion in open channels as completely as possible. Analytical solutions are presented as a combination of exponential functions, hyperbolic functions, and infinite series. The obtained analytical solution was further applied to the simulation of flood path and morphological evolution in the Lower Yellow River. The phenomenon of increased peak discharge in the downstream reach was successfully simulated. It was encouraging that the results were in good agreement with the observed data. [ABSTRACT FROM AUTHOR]

Evolutionary robot systems are usually affected by the properties of the environment indirectly through selection. In this paper, we present and investigate a system where the environment also has a direct effect—through regulation. We propose a novel robot encoding method where a genotype encodes multiple possible phenotypes, and the incarnation of a robot depends on the environmental conditions taking place in a determined moment of its life. This means that the morphology, controller, and behavior of a robot can change according to the environment. Importantly, this process of development can happen at any moment of a robot's lifetime, according to its experienced environmental stimuli. We provide an empirical proof-of-concept, and the analysis of the experimental results shows that environmental regulation improves adaptation (task performance) while leading to different evolved morphologies, controllers, and behavior.

Butterfly wing patterns have emerged as exceptional model systems with which to link the developmental and genetic processes that generate morphological variation with the ecological and evolutionary processes that shape this variation in natural populations. Among butterflies, research on species within the genus Heliconius has provided remarkable opportunities to explore how phenotypic diversity is generated within the context of an extraordinary adaptive radiation. Wing pattern diversity among the 48 species and hundreds of intraspecific variants arose within the last 12–14 million years and includes striking pattern convergence between distantly related species, as well as marked pattern divergence between closely related populations and species. Here, we synthesize recent research aimed at gaining a mechanistic understanding of how this variation is generated. This research integrates decades of controlled crossing experiments, and the discovery of major wing patterning genes (optix, aristaless1, WntA and cortex) with recent functional genetic manipulation using CRISPR/Cas9 targeted mutagenesis. The emerging data provides a rich framework with which to explore the repeatability of evolution, particularly within the context of how natural selection acts on divergent gene regulatory networks to generate both highly convergent, as well as highly divergent phenotypes. Overall, the functional data show that the gene regulatory networks underlying pattern variation diverge rapidly in Heliconius; yet these networks retain enough flexibility so that natural selection can drive the evolution of nearly identical patterns from different developmental genetic starting points. Moreover, for the first time this research is starting to illuminate the links between the genetic changes modulating pattern variation and how they influence the larger gene networks that are ultimately responsible for patterning a butterfly wing. There are still large gaps in our understanding, but current research priorities are well laid out and experimental methodologies are in place to address them. The challenge is to synthesize diverse research strategies into a cohesive picture of morphological evolution.

In the age of phylogenetic comparative methods, evolutionary biologists have been able to explore evolutionary trends in form in unique and extraordinarily diverse groups of animals. Pleuronectiformes, commonly known as flatfishes, is a diverse and specialized order of fishes that have remarkable asymmetry induced by ocular migration and a benthic life style. Although flatfishes are unique from other fishes, species within the group are morphologically diverse. The origin of ocular migration has been a primary focus of research; however, little is known about overall shape diversification among the flatfishes. In this study, we use integrative methods to examine how body shape evolved within the flatfishes. Shape was quantified from X-rays using geometric morphometrics for 389 individuals across 145 species. The most recent and robust phylogeny was overlaid onto the morphospace and phylogenetic signal was calculated to ascertain convergence in the morphospace. In addition, phylogenetic linear models were employed to determine if ecological traits were correlated with shape and if size had an effect on overall body shape. Results revealed that the majority of variation evolved recently, within the past 15–10-million-years in the middle Miocene, and is highly variable within the flatfishes. These changes are best summarized by body depth, jaw length and medial fin length. Dorsal and anal fin length are correlated, which may be due to the unique mode of locomotion used by flatfishes. A phylogenetic linear model and phylomorphospace analysis suggested that several ecological traits are correlated with shape, which indicates an ecological role in the diversification of flatfishes.

Traits that appear discontinuously along phylogenies may be explained by independent origins (homoplasy) or repeated loss (homology). While discriminating between these models is difficult, the dissection of gene regulatory networks (GRNs) which drive the development of such repeatedly occurring traits can offer a mechanistic window on this fundamental problem. The GRN responsible for the male-specific pattern of Drosophila (D.) melanogaster melanic tergite pigmentation has received considerable attention. In this system, a metabolic pathway of pigmentation enzyme genes is expressed in spatial and sex-specific (i.e., dimorphic) patterns. The dimorphic expression of several genes is regulated by the Bab transcription factors, which suppress pigmentation enzyme expression in females, by virtue of their high expression in this sex. Here, we analyzed the phylogenetic distribution of species with male-specific pigmentation and show that this dimorphism is phylogenetically widespread among fruit flies. The analysis of pigmentation enzyme gene expression in distantly related dimorphic and monomorphic species shows that dimorphism is driven by the similar deployment of a conserved metabolic pathway. However, sexually dimorphic Bab expression was found only in D. melanogaster and its close relatives. These results suggest that dimorphism evolved by parallel deployment of differentiation genes, but was derived through distinct architectures at the level of regulatory genes. This work demonstrates the interplay of constraint and flexibility within evolving GRNs, findings that may foretell the mechanisms of homoplasy more broadly.

The collaborative planning of a wind-photovoltaic (PV)-energy storage system (ESS) is an effective means to reduce the carbon emission of system operation and improve the efficiency of resource collaborative utilization. In this paper, a wind-PV-ESS collaborative planning strategy considering the morphological evolution of the transmission and distribution network is proposed. Firstly, aiming at the optimal economy of transmission and distribution network and considering the constraints of safe and stable operation of the system, the planning model of the transmission network based on DC power flow and the planning model of the distribution network based on AC power flow are constructed. Further, considering the coupling interaction between the transmission and distribution networks, a collaborative planning model of transmission and distribution networks based on second-order cone relaxation (SOCR) is constructed. Secondly, in order to reduce the computational complexity of the model and ensure the global optimality of the model solution, a fast model solution method based on heterogeneous decomposition architecture is proposed. Thirdly, the multiple driving factors of the morphological evolution of transmission and distribution network are analyzed, the morphological evolution path and typical characteristics of transmission and distribution network are determined, and a wind-PV-ESS collaborative planning strategy considering the morphological evolution of a transmission and distribution network is proposed. Finally, the results show that, compared with the sprouting period, the overall economy of the development period and maturity period is improved by 3342 k$ and 5751 k$ respectively, and the effectiveness and necessity of the collaborative planning strategy proposed in this paper is verified.

The radial sand ridges consist of more than 70 sand ridges that are spread out radially on the continental shelf of the South Yellow Sea. As a unique geomorphological feature in the world, its evolution process and characteristics are crucial to marine resource management and ecological protection. Based on the multi-source remote sensing image data from 1979 to 2019, three types of geomorphic feature lines, artificial coastlines, waterlines, and sand ridge lines were extracted. Using the GIS sequence analysis method (Digital Shoreline Analysis System (DSAS), spatial overlay analysis, standard deviational ellipse method), the evolution characteristics of the shoreline, exposed tidal flats, and underwater sand ridges from land to sea were interpreted. The results demonstrate that: (1) The coastline has been advancing towards the sea with a maximum advance rate of 348.76 m/a from Wanggang estuary to Xiaoyangkou Port. (2) The exposed tidal flats have decreased by 1484 km2 including the reclaimed area of 1414 km2 and showed a trend of erosion in the north around Xiyang channel and deposition in the southeast around the Gaoni and Jiangjiasha areas. (3) The overall sand ridge lines showed a trend of gradually moving southeast (135°), and the moving distance is nearly 4 km in the past 40 years. In particular, the sand ridge of Tiaozini has moved 11 km southward, while distances of 8 km for Liangyuesha and 5 km for Lengjiasha were also observed. For the first time, this study quantified the overall migration trend of the RSRs. The imbalance of the regional tidal wave system may be one of the main factors leading to the overall southeastward shift of the radiation sandbanks.

Abstract Background A number of shelled and shell-less gastropods are known to use multiple defensive mechanisms, including internally generated or externally obtained biochemically active compounds and structures. Within Nudipleura, nudibranchs within Cladobranchia possess such a special defense: the ability to sequester cnidarian nematocysts – small capsules that can inject venom into the tissues of other organisms. This ability is distributed across roughly 600 species within Cladobranchia, and many questions still remain in regard to the comparative morphology and evolution of the cnidosac – the structure that houses sequestered nematocysts (called kleptocnides). In this paper, we describe cnidosac morphology across the main groups of Cladobranchia in which it occurs, and place variation in its structure in a phylogenetic context to better understand the evolution of nematocyst sequestration. Results Overall, we find that the length, size and structure of the entrance to the cnidosac varies more than expected based on previous work, as does the structure of the exit, the musculature surrounding the cnidosac, and the position and orientation of the kleptocnides. The sequestration of nematocysts has originated at least twice within Cladobranchia based on the phylogeny presented here using 94 taxa and 409 genes. Conclusions The cnidosac is not homologous to cnidosac-like structures found in Hancockiidae. Additionally, the presence of a sac at the distal end of the digestive gland may have originated prior to the sequestration of nematocysts. This study provides a more complete picture of variation in, and evolution of, morphological characters associated with nematocyst sequestration in Cladobranchia.

Abstract Background Populations that have repeatedly colonized novel environments are useful for studying the role of ecology in adaptive divergence – particularly if some individuals persist in the ancestral habitat. Such “contemporary ancestors” can be used to demonstrate the effects of selection by comparing phenotypic and genetic divergence between the derived population and their extant ancestors. However, evolution and demography in these “contemporary ancestors” can complicate inferences about the source (standing genetic variation, de novo mutation) and pace of adaptive divergence. Marine threespine stickleback (Gasterosteus aculeatus) have colonized freshwater environments along the Pacific coast of North America, but have also persisted in the marine environment. To what extent are marine stickleback good proxies of the ancestral condition? Results We sequenced > 5800 variant loci in over 250 marine stickleback from eight locations extending from Alaska to California, and phenotyped them for platedness and body shape. Pairwise FST varied from 0.02 to 0.18. Stickleback were divided into five genetic clusters, with a single cluster comprising stickleback from Washington to Alaska. Plate number, Eda, body shape, and candidate loci showed evidence of being under selection in the marine environment. Comparisons to a freshwater population demonstrated that candidate loci for freshwater adaptation varied depending on the choice of marine populations. Conclusions Marine stickleback are structured into phenotypically and genetically distinct populations that have been evolving as freshwater stickleback evolved. This variation complicates their usefulness as proxies of the ancestors of freshwater populations. Lessons from stickleback may be applied to other “contemporary ancestor”-derived population studies.

Abstract Background Morphological convergence triggered by trophic adaptations is a common pattern in adaptive radiations. The study of shape variation in an evolutionary context is usually restricted to well-studied fish models. We take advantage of the recently revised systematics of New World Ariidae and investigate skull shape evolution in six genera of northern Neotropical Ariidae. They constitute a lineage that diversified in the marine habitat but repeatedly adapted to freshwater habitats. 3D geometric morphometrics was applied for the first time in catfish skulls and phylogenetically informed statistical analyses were performed to test for the impact of habitat on skull diversification after habitat transition in this lineage. Results We found that skull shape is conserved throughout phylogeny. A morphospace analysis revealed that freshwater and marine species occupy extreme ends of the first principal component axis and that they exhibit similar Procrustes variances. Yet freshwater species occupy the smallest shape space compared to marine and brackish species (based on partial disparity), and marine and freshwater species have the largest Procrustes distance to each other. We observed a single case of shape convergence as derived from ‘C-metrics’, which cannot be explained by the occupation of the same habitat. Conclusions Although Ariidae occupy such a broad spectrum of different habitats from sea to freshwater, the morphospace analysis and analyses of shape and co-variation with habitat in a phylogenetic context shows that conservatism dominates skull shape evolution among ariid genera.

Wing pattern evolution in Heliconius butterflies provides some of the most striking examples of adaptation by natural selection. The genes controlling pattern variation are classic examples of Mendelian loci of large effect, where allelic variation causes large and discrete phenotypic changes and is responsible for both convergent and highly divergent wing pattern evolution across the genus. We characterize nucleotide variation, genotype-by-phenotype associations, linkage disequilibrium (LD), and candidate gene expression patterns across two unlinked genomic intervals that control yellow and red wing pattern variation among mimetic forms of Heliconius erato. Despite very strong natural selection on color pattern, we see neither a strong reduction in genetic diversity nor evidence for extended LD across either patterning interval. This observation highlights the extent that recombination can erase the signature of selection in natural populations and is consistent with the hypothesis that either the adaptive radiation or the alleles controlling it are quite old. However, across both patterning intervals we identified SNPs clustered in several coding regions that were strongly associated with color pattern phenotype. Interestingly, coding regions with associated SNPs were widely separated, suggesting that color pattern alleles may be composed of multiple functional sites, conforming to previous descriptions of these loci as "supergenes." Examination of gene expression levels of genes flanking these regions in both H. erato and its co-mimic, H. melpomene, implicate a gene with high sequence similarity to a kinesin as playing a key role in modulating pattern and provides convincing evidence for parallel changes in gene regulation across co-mimetic lineages. The complex genetic architecture at these color pattern loci stands in marked contrast to the single casual mutations often identified in genetic studies of adaptation, but may be more indicative of the type of genetic changes responsible for much of the adaptive variation found in natural populations.

Abstract Background Antennae are multi-segmented appendages and main odor-sensing organs in insects. In Lepidoptera (moths and butterflies), antennal morphologies have diversified according to their ecological requirements. While diurnal butterflies have simple, rod-shaped antennae, nocturnal moths have antennae with protrusions or lateral branches on each antennal segment for high-sensitive pheromone detection. A previous study on the Bombyx mori (silk moth) antenna, forming two lateral branches per segment, during metamorphosis has revealed the dramatic change in expression of antennal patterning genes to segmentally reiterated, branch-associated pattern and abundant proliferation of cells contributing almost all the dorsal half of the lateral branch. Thus, localized cell proliferation possibly controlled by the branch-associated expression of antennal patterning genes is implicated in lateral branch formation. Yet, actual gene function in lateral branch formation in Bombyx mori and evolutionary mechanism of various antennal morphologies in Lepidoptera remain elusive. Results We investigated the function of several genes and signaling specifically in lateral branch formation in Bombyx mori by the electroporation-mediated incorporation of siRNAs or morpholino oligomers. Knock down of aristaless, a homeobox gene expressed specifically in the region of abundant cell proliferation within each antennal segment, during metamorphosis resulted in missing or substantial shortening of lateral branches, indicating its importance for lateral branch formation. aristaless expression during metamorphosis was lost by knock down of Distal-less and WNT signaling but derepressed by knock down of Notch signaling, suggesting the strict determination of the aristaless expression domain within each antennal segment by the combinatorial action of them. In addition, analyses of pupal aristaless expression in antennae with various morphologies of several lepidopteran species revealed that the aristaless expression pattern has a striking correlation with antennal shapes, whereas the segmentally reiterated expression pattern was observed irrespective of antennal morphologies. Conclusions Our results presented here indicate the significance of aristaless function in lateral branch formation in B. mori and imply that the diversification in the aristaless expression pattern within each antennal segment during metamorphosis is one of the significant determinants of antennal morphologies. According to these findings, we propose a mechanism underlying development and evolution of lepidopteran antennae with various morphologies.

Coastal depositional environments are the site of complex interactions between continental and marine processes. Barrier islands are highly dynamic coastal systems, typical of these transitional environments, and are affected by sea level changes and the accumulation of transgressive deposits with the landwards migration of the coast. The offshore of Marzamemi (Syracuse Province, Sicily), in the south-eastern portion of the Hyblean foreland, represents an excellent site for the study of transgressive deposits and their connection with the sea-level changes. The available dataset consisted of new high-resolution bathymetry (Multibeam), whose description and interpretation through a Digital Elevation Model (DEM) was integrated with a grid of eighteen seismic profiles (SPARKER). In the investigated bathymetric range, from about −5 m to −60 m, a sensibly different morphological setting between the northern and southern sectors was evident. Within the whole study area, three bathymetric contours (−45 m, −35 m and −20 m) were identified and assumed as the markers of the main locations of the paleo-coastlines during the recent changes in the sea level. Along the northern sector, three submerged barrier-lagoon systems developed on a calcarenite substratum, marking important steps of the Late Quaternary sea-level rise. They coexisted with numerous karst forms (poljes and dolines). In the southern sector the transgressive environmental evolution was significantly different and submerged lagoons did not form. Here the outcropping calcarenite substratum was affected by the development of paleo-rivers and karsts structures, a tract in common along with many Mediterranean carbonate coastal areas.

Rock daisies (Perityleae; Compositae) are a diverse clade of seven genera and ca. 84 minimum‐rank taxa that mostly occur as narrow endemics on sheer rock cliffs throughout the southwest United States and northern Mexico. Taxonomy of Perityleae has traditionally been based on morphology and cytogenetics. To test taxonomic hypotheses and utility of characters emphasized in past treatments, we present the first densely sampled molecular phylogenies of Perityleae and reconstruct trait and chromosome evolution. We inferred phylogenetic trees from whole chloroplast genomes, nuclear ribosomal cistrons, and hundreds of low‐copy nuclear genes using genome skimming and target capture. Discordance between sources of molecular data suggests an underappreciated history of hybridization in Perityleae. Phylogenies support the monophyly of subtribe Peritylinae, a distinctive group possessing a four‐lobed disc corolla; however, all of the phylogenetic trees generated in this study reject the monophyly of the most species‐rich genus, Perityle, as well as its sections: Perityle sect. Perityle, Perityle sect. Laphamia, and Perityle sect. Pappothrix. Using reversible jump MCMC, our results suggest that morphological characters traditionally used to classify members of Perityleae have evolved multiple times within the group. A base chromosome number x = 9 gave rise to higher base numbers in subtribe Peritylinae (x = 12, 13, 16, 17, 18, and 19) through polyploidization, followed by ascending or descending dysploidy. Most taxa constitute a monophyletic lineage with a base chromosome number of x = 17, with multiple neo‐polyploidization events. These results demonstrate the advantages and obstacles of next‐generation sequencing approaches in synantherology while laying the foundation for taxonomic revision and comparative study of the evolutionary ecology of Perityleae. [ABSTRACT FROM AUTHOR]

Aim: Morphological and lineage evolution are predicted to follow different patterns in island and mainland radiations. However, the extent to which these geographical contexts influence evolutionary trajectories remains poorly understood, in part because few studies have focused on species‐rich clades colonizing continents and archipelagos over comparable timeframes. Focusing on a diverse songbird clade radiating on the African continent and adjacent islands, we tested whether morphological evolution is best explained by adaptive or non‐adaptive processes, and whether mainland and island lineages evolved to occupy different regions or volumes of morphological space (morphospace). Location: Palaeotropics, with a particular focus on the Afrotropical region. Taxon: White‐eyes, Zosterops (Aves: Zosteropidae). Methods: We generated principal component axes from novel trait data for 120 species and combined this information with a comprehensive dated phylogeny. We then analysed the dynamics of trait and lineage diversification using comparative evolutionary methods. Results: An early burst and slowdown pattern of lineage accumulation is not mirrored by phenotypic evolution, which instead shows an apparent convergence on particular phenotypes. However, the overall signature of phenotypic convergence is strongly driven by mainland taxa, in which phenotypes appear to be highly constrained within elevational zones, while speciation events are often associated with phenotypic divergence from one body plan to the other after colonization of highland from lowland habitats, or vice versa. By contrast, island lineages have repeatedly explored novel areas of morphospace with patterns of phenotypic divergence generally not distinguishable from a random‐walk model. Main conclusions: Diversification of Zosterops highlights contrasting evolutionary trends and dynamics for continental versus island species. We suggest the different trajectory of evolution in insular lineages arises from reduced species competition leading to an increase in ecological opportunity, thereby providing a release to phenotypic constraints experienced by continental taxa. [ABSTRACT FROM AUTHOR]

Sedum, containing approximately 470 species, is by far the largest genus of Crassulaceae. Three decades of molecular phylogenetic work have provided evidence for the non‐monophyly of Sedum and many more of the 30 genera of Crassulaceae subfam. Sempervivoideae. In this study, we present a broadly sampled and dated molecular phylogeny of Sempervivoideae including 80% of all infrageneric taxa described in Sedum as well as most other genera of the subfamily. We used sequences of one nuclear (ITS) and three plastid markers (matK, rps16, trnL‐trnF). The five major lineages of Sempervivoideae (i.e., Telephium clade, Petrosedum clade, Sempervivum/Jovibarba, Aeonium clade, Leucosedum plus Acre clades) were resolved as successive sister to each other in the phylogenetic analysis of the plastid markers, while in the ITS phylogeny the Petrosedum clade is the closest relative of the Aeonium clade. Our dating analysis of ITS suggests that Sempervivoideae diversified rapidly throughout the Paleocene and Eocene, possibly in the area of the former Tethys and Paratethys archipelago. A biogeographic pattern emerges in which migration out of this ancestral area is linked to pronounced morphological evolution resulting in several distinct lineages recognized as segregate genera thought to be derived from Sedum. These segregate genera, however, have been defined on the basis of strongly homoplasious characters such as degree of petal fusion, petal colouration or flower merism. Moreover, all character states currently used for the delimitation of segregate genera seem to be homoplasious, and each of them can be found in at least one species of Sedum. Extensive literature work led to the conclusion that only few of the monophyletic clades found by us can be defined unambiguously by morphological characters. Mainly for these two reasons, we believe that combination of all 14 genera currently recognized in tribe Sedeae (= clades Leucosedum plus Acre) into Sedum might be the most stable solution of the "Sedum problem". This new Sedum s.l. would then comprise approximately 755 species. [ABSTRACT FROM AUTHOR]

Frogs and toads (Amphibia: Anura) display diverse ecologies and behaviours, which are often correlated with visual capacity in other vertebrates. Additionally, anurans exhibit a broad range of relative eye sizes, which have not previously been linked to ecological factors in this group. We measured relative investment in eye size and corneal size for 220 species of anurans representing all 55 currently recognized families and tested whether they were correlated with six natural history traits hypothesized to be associated with the evolution of eye size. Anuran eye size was significantly correlated with habitat, with notable decreases in eye investment among fossorial, subfossorial and aquatic species. Relative eye size was also associated with mating habitat and activity pattern. Compared to other vertebrates, anurans have relatively large eyes for their body size, indicating that vision is probably of high importance. Our study reveals the role that ecology and behaviour may have played in the evolution of anuran visual systems and highlights the usefulness of museum specimens, and importance of broad taxonomic sampling, for interpreting macroecological patterns. [ABSTRACT FROM AUTHOR]

Allometric trends in the degree of sexual dimorphism with body size have long fascinated evolutionary biologists. Many male‐biased clades display more prominent sexual dimorphism in larger taxa (Rensch's rule), with most examples documenting this pattern for body size dimorphism. Although sexual dimorphism in traits other than body size is equally functionally relevant, characterizing allometric patterns of sexual dimorphism in such traits is hampered by lack of an analytical framework that can accommodate multivariate phenotypes. In this article, we derive a multivariate equivalency for investigating trends in sexual dimorphism—relative to overall body size—across taxa and provide a generalized test to determine whether such allometric patterns correspond with Rensch's rule. For univariate linear traits such as body size, our approach yields equivalent results to those from standard procedures, but our test is also capable of detecting trends in multivariate datasets such as shape. Computer simulations reveal that the method displays appropriate statistical properties, and an empirical example in Mediterranean lizards provides the first demonstration of Rensch's rule in a multivariate phenotype (head shape). Our generalized procedure substantially extends the analytical toolkit for investigating macroevolutionary patterns of sexual dimorphism and seeking a better understanding of the processes that underlie them. [ABSTRACT FROM AUTHOR]

During approximate 773 K aging treatment of 100Mn13 steel, degenerate pearlite will occur and evolve into lamellar pearlite during growth process. The microstructures of degenerate pearlite and its evolutionary lamellar pearlite are observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results show that after 748 K, 773 K and 798 K aging, degenerate pearlites occur at grain boundary. At growth front of degenerate pearlite forming at 773 K and 798 K, pearlite presents a morphology of short lamellae of carbide and ferrite, indicating a trend of developing into lamellar pearlite. The higher the temperature is, the more obvious the trend is, and even a conventional lamellar pearlite has developed. However, there is no morphological evolution for degenerate pearlite forming at 748 K aging. Besides, the constituents of degenerate pearlite is identified as M23C6 and ferrite, and Kurdjumov-Sachs orientation relationship exists between them, (011̅)α//(1.11̅)M23C6, [111]α//[110]M23C6. This orientation relationship maintains in morphological evolution from degenerate pearlite to lamellar pearlite. [ABSTRACT FROM AUTHOR]

Diversification and adaptive radiations are tied to evolvability, which in turn is linked to morphological integration. Tightly integrated structures typically evolve in unison, whereas loosely integrated structures evolve separately. Highly integrated structures are therefore thought to constrain evolutionary change by limiting morphological disparity. Mounting evidence suggests that high integration may facilitate evolutionary change along a single trajectory. We used geometric morphometrics to compare cranial disparity and integration among phyllostomid bats—which exhibit the greatest dietary diversity of any mammalian family— and their sister taxa within the superfamily Noctilionoidea. Our results reveal that phyllostomids are more tightly integrated and have less disparity in cranial shape than their outgroups, despite exhibiting tenfold higher species richness and significantly increased rates of speciation. Phyllostomid cranial morphology appears to have diverged from that of other noctilionoids by evolving along a single axis of morphological variation that describes the relative length of the rostrum. We propose that phyllostomids were able to evolve to occupy a wide range of dietary niches by varying rostrum length, possibly along a line of least evolutionary resistance. This study provides a compelling empirical example of how increased integration can lead to adaptation, implying that both high and low integration can underlie diverse phenotypes in adaptive radiation. [ABSTRACT FROM AUTHOR]

Although the evolution and diversification of flowers is often attributed to pollinator‐mediated selection, interactions between co‐occurring plant species can alter patterns of selection mediated by pollinators and other agents. The extent to which both floral density and congeneric species richness affect patterns of net and pollinator‐mediated selection on multiple co‐occurring species in a community is unknown and is likely to depend on whether co‐occurring plants experience competition or facilitation for reproduction. We conducted an observational study of selection on four species of Clarkia (Onagraceae) and tested for pollinator‐mediated selection on two Clarkia species in communities differing in congeneric species richness and local floral density. When selection varied with community context, selection was generally stronger in communities with fewer species, where local conspecific floral density was higher, and where local heterospecific floral density was lower. These patterns suggest that intraspecific competition at high densities and interspecific competition at low densities may affect the evolution of floral traits. However, selection on floral traits was not pollinator mediated in Clarkia cylindrica or Clarkia xantiana, despite variation in pollinator visitation and the extent of pollen limitation across communities for C. cylindrica. As such, interactions between co‐occurring species may alter patterns of selection mediated by abiotic agents of selection. [ABSTRACT FROM AUTHOR]

Delimiting species boundaries is a complex challenge usually hindered by overlooked morphological diversification or misinterpretation of geographically structured phenotypic variability. Independent molecular data are extremely useful to characterize and understand such morphological diversity. Morphological and molecular variability of the non-phoretic and apterous, widely distributed, giant blister beetles of the genus Berberomeloe , were investigated within and between lineages across most of the distributional range of the genus. We used two mtDNA gene fragments to characterize genetic variability and to produce a time-calibrated phylogeny of the genus. Our results reveal several mitochondrial lineages, allopatrically, parapatrically and sympatrically distributed. Most clades are not distinguishable between each other based on morphometrics. However, no morphometric overlap is observed between two closely related clades, one of them occurring in sympatry with a distantly congeneric species (B. insignis), suggesting that sympatry could trigger morphological diversification. Although most species share a morphometric space, they can be morphologically identified by a combination of easily observed characteristic qualitative features. Based on the concordance between mtDNA clades and morphological units, we describe six new species of Berberomeloe (B. castuo sp. nov. B. comunero sp. nov. B. indalo sp. nov, B. yebli sp. nov. B. payoyo sp. nov. and B. tenebrosus sp. nov.), revalidate two taxa (B. maculifrons comb. nov. and B. laevigatus comb. nov.) and redefine B. majalis. [ABSTRACT FROM AUTHOR]

Background: Adaptive radiations are characterized by extreme and/or iterative phenotypic divergence; however, such variation does not accumulate evenly across an organism. Instead, it is often partitioned into sub-units, or modules, which can differentially respond to selection. While it is recognized that changing the pattern of modularity or the strength of covariation (integration) can influence the range or rate of morphological evolution, the relationship between shape variation and covariation remains unclear. For example, it is possible that rapid phenotypic change requires concomitant changes to the underlying covariance structure. Alternatively, repeated shifts between phenotypic states may be facilitated by a conserved covariance structure. Distinguishing between these scenarios will contribute to a better understanding of the factors that shape biodiversity. Here, we explore these questions using a diverse Lake Malawi cichlid species complex, Tropheops, that appears to partition habitat by depth. Results: We construct a phylogeny of Tropheops populations and use 3D geometric morphometrics to assess the shape of four bones involved in feeding (mandible, pharyngeal jaw, maxilla, pre-maxilla) in populations that inhabit deep versus shallow habitats. We next test numerous modularity hypotheses to understand whether fish at different depths are characterized by conserved or divergent patterns of modularity. We further examine rates of morphological evolution and disparity between habitats and among modules. Finally, we raise a single Tropheops species in environments mimicking deep or shallow habitats to discover whether plasticity can replicate the pattern of morphology, disparity, or modularity observed in natural populations. Conclusions: Our data support the hypothesis that conserved patterns of modularity permit the evolution of divergent morphologies and may facilitate the repeated transitions between habitats. In addition, we find the lab-reared populations replicate many trends in the natural populations, which suggests that plasticity may be an important force in initiating depth transitions, priming the feeding apparatus for evolutionary change. [ABSTRACT FROM AUTHOR]

In this study, the morphological evolution and sintering properties of the palygorskite nanofibers were studied along with the increase of temperature, using raw palygorskite as materials. The palygorskite powder was calcined at different temperatures in the range of 100°C‐1200°C, and the microstructural evolution of the palygorskite nanofibers was investigated by thermogravimetric and differential thermal analysis (TG‐DTA), X‐ray diffraction (XRD), scanning electron microscopy (SEM), and high‐resolution transmission electron microscope (HRTEM). Furthermore, the palygorskite powder was shaped to bars by dry pressing and sintered from 700°C to 1200°C. The properties of the sintered palygorskite were characterized by bending strength, mercury intrusion porosimeter (MIP), and stepwise isothermal dilatometry (SID). The results showed that the morphology of palygorskite nanofibers maintained unchanged till 1000°C. The palygorskite nanofibers molted to bind each other and formed a solid interwoven network structure at 1100°C. Correspondingly, it was shown from the sharply decrease of the sintered palygorskite porosity from 45.46% at 1000°C to 1.82% at 1100°C that the dense sintering of palygorskite started at 1100°C. With the sintering proceeding, some closed micropores fused each other to form bigger opening pores, resulting in a slight increase of porosity at 1200°C. However, the pore size distribution got more uniform and the density of the sintered body increased. So the bending strength of the sintered body reached the maximum of 176.67 Mpa and finally the main crystalline phases of the sintered sample changed to quartz, enstatite, and kyanite. The sintering activation energy of the palygorskite was measured by means of SID with a value of 906.46 kJ·mol−1. [ABSTRACT FROM AUTHOR]

How random DNA mutations have established the diverse morphology of extant vertebrates is one of the major challenges in evolutionary biology. Thanks to the recent advancement in DNA sequencing technologies, the genome sequences of many non‐model species have been determined, which allows us to address previously inaccessible questions about gene regulatory evolution in vertebrates. In particular, the genome sequences of non‐teleost ray‐finned fishes and cartilaginous fishes offer clues about when and how vertebrates gained developmental enhancers related to morphological traits that were required for the water‐to‐land transition. In this review, I examine the evolutionary origin of conserved non‐coding elements (CNEs), which often function as tissue‐specific developmental enhancers, and discuss how CNEs are related to gene regulatory changes that caused the major morphological transitions of vertebrates. [ABSTRACT FROM AUTHOR]

Domesticated animals display suites of altered morphological, behavioral, and physiological traits compared to their wild ancestors, a phenomenon known as the domestication syndrome (DS). Because these alterations are observed to co‐occur across a wide range of present day domesticates, the traits within the DS are assumed to covary within species and a single developmental mechanism has been hypothesized to cause the observed co‐occurrence. However, due to the lack of formal testing it is currently not well‐resolved if the traits within DS actually covary. Here, we test the hypothesis that the presence of the classic morphological domestication traits white pigmentation, floppy ears, and curly tails predict the strength of behavioral correlations in support of the DS in 78 dog breeds. Contrary to the expectations of covariation among DS traits, we found that morphological traits did not covary among themselves, nor did they predict the strength of behavioral correlations among dog breeds. Further, the number of morphological traits in a breed did not predict the strength of behavioral correlations. Our results thus contrast with the hypothesis that the DS arises due to a shared underlying mechanism, but more importantly, questions if the morphological traits embedded in the DS are actual domestication traits or postdomestication improvement traits. For dogs, it seems highly likely that strong selection for breed specific morphological traits only happened recently and in relation to breed formation. Present day dogs therefore have limited bearing of the initial selection pressures applied during domestication and we should reevaluate our expectations of the DS accordingly. [ABSTRACT FROM AUTHOR]

One of the most striking radiations in brachyuran evolution is the considerable morphological diversification of the external reproductive structures of primary freshwater crabs: the male first gonopod (G1) and the female vulva (FV). However, the lack of quantitative studies, especially the lack of data on female genitalia, has seriously limited our understanding of genital evolution in these lineages. Here we examined 69 species of the large Chinese potamid freshwater crab genus Sinopotamon Bott, 1967 (more than 80% of the described species). We used a landmark‐based geometric morphometric approach to analyze variation in the shape of the G1 and FV, and to compare the relative degree of variability of the genitalia with non‐reproductive structures (the third maxillipeds). We found rapid divergent evolution of the genitalia among species of Sinopotamon when compared to non‐reproductive traits. In addition, the reconstruction of ancestral groundplans, together with plotting analyses, indicated that the FV show the most rapid divergence, and that changes in FV traits correlate with changes in G1 traits. Here we provide new evidence for coevolution between the male and female external genitalia of Sinopotamon that has likely contributed to rapid divergent evolution and an associated burst of speciation in this lineage. [ABSTRACT FROM AUTHOR]

Primate limb morphology is often described as either generalized, that is, suited to a range of locomotor and positional behaviors, or specialized for unique locomotor behaviors such as brachiation or bipedalism. The evolution of highly specialized limb morphology may result in loss of evolvability, that is, in a decreased capacity of the locomotor skeleton to evolve in response to selection towards alternative ecomorphological niches. Using evolutionary simulations, I show that the highly specialized limb anatomy of hominoids is associated with a significant loss of evolvability, defined as the number of generations to reach alternative adaptive peaks, and in parallel an increased risk of extinction, particularly in simulated evolution toward generalized quadrupedal limb proportions. Loss of evolvability in apes and humans correlates with three factors: (1) decreased correlation among limb bone lengths (i.e., integration), which slows the rate of change along lines of least evolutionary resistance; (2) limb specialization, which places apes and humans in relatively remote areas of morphospace; and (3) increased skeletal size as a proxy for body size. Thus, locomotor over‐specialization can lead to evolutionary dead‐ends that significantly increase the probability of hominoid populations going extinct before evolving new adaptive morphologies. [ABSTRACT FROM AUTHOR]

The Neotropical bat genus Platyrrhinus is one of the most speciose genera in the family Phyllostomidae. It includes 20 species of frugivorous bats that are endemic to the Neotropics. Platyrrhinus chocoensis is a species distributed from southern Panama southward into northern Ecuador on the lowlands and mid elevations of the Pacific coast of northern South America, across the Chocó Biogeographic region, a hot-spot of biodiversity, extending west of the Andes. Due to its association with the Chocoan rainforest, P. chocoensis was designated as one of the two species of bats included in the Red List of Colombian mammals. Another species of Platyrrhinus, P. dorsalis, also occurs in Colombia and Ecuador along both slopes of the Andes. In contrast with P. chocoensis, P. dorsalis is considered a relatively common bat species within the Least Concern category of the International Union for Conservation of Nature. We evaluate the systematics and taxonomy of both species, P. chocoensis and P. dorsalis, using a combination of morphometric and molecular analyses. Although our morphometric analyses support a consistent morphological differentiation between these two taxa, the analyses of mtDNA sequences of Cyt-b and ND2 revealed that P. chocoensis and P. dorsalis have no differentiation at the genetic level for the analyzed markers (0.7%); therefore, we recognize P. chocoensis as a junior synonym of P. dorsalis. In addition, our results support the recognition of two subspecies within P. dorsalis: the nominate P. d. dorsalis and P. d. chocoensis. Finally, we discuss the biogeographic implications of our findings, in particular the consequences of the synonymization of P. chocoensis, that also warrants the reevaluation of the taxonomic and conservation status of P. dorsalis populations from the Chocoan domain. [ABSTRACT FROM AUTHOR]

In the age of phylogenetic comparative methods, evolutionary biologists have been able to explore evolutionary trends in form in unique and extraordinarily diverse groups of animals. Pleuronectiformes, commonly known as flatfishes, is a diverse and specialized order of fishes that have remarkable asymmetry induced by ocular migration and a benthic life style. Although flatfishes are unique from other fishes, species within the group are morphologically diverse. The origin of ocular migration has been a primary focus of research; however, little is known about overall shape diversification among the flatfishes. In this study, we use integrative methods to examine how body shape evolved within the flatfishes. Shape was quantified from X-rays using geometric morphometrics for 389 individuals across 145 species. The most recent and robust phylogeny was overlaid onto the morphospace and phylogenetic signal was calculated to ascertain convergence in the morphospace. In addition, phylogenetic linear models were employed to determine if ecological traits were correlated with shape and if size had an effect on overall body shape. Results revealed that the majority of variation evolved recently, within the past 15–10-million-years in the middle Miocene, and is highly variable within the flatfishes. These changes are best summarized by body depth, jaw length and medial fin length. Dorsal and anal fin length are correlated, which may be due to the unique mode of locomotion used by flatfishes. A phylogenetic linear model and phylomorphospace analysis suggested that several ecological traits are correlated with shape, which indicates an ecological role in the diversification of flatfishes. [ABSTRACT FROM AUTHOR]

Gully headcut erosion become the main cause for the longitudinal and horizontal surface, surface fragmentation and area shrinkage in the gully region of the Loess Plateau in China,threatening the development of local agriculture, economy and ecological environment. At present, the vegetation has played an important role in controlling the gully headcut erosion in this region. Most of the previous studies mainly focused on the influence of the above-ground parts of the vegetation on the basin and even the regional scale.However, little is known about the effects of the root density of vegetation on the gully headcut erosion. The effects of sediment production and its morphological evolution are also lacking, particularly on the loess. Therefore, a combined experiment of simulated rainfall and runoff scouring was conducted to explore the effects of the grass (Agropyron cristatum) root densities on the erosion process and morphological evolution during the gully headcut erosion. The experimental plot consist of the upstream area, gully head and gully bed. The length and width of the upstream area were 8.0 m and 1.5 m. The gully head wall height was 1.2 m. The length and width of the gully bed were 1.0 m and 1.5 m. The slope of the upstream area was consistent with that of the gully bed, being set as 3°.Compared to the bare land experiment area, this paper presents the process of soil loss, gully head retreat, gully cutting and development, in the different root density around the experiment area of Agropyron cristatum (planting space: 20 cm×20 cm, C1; 15 cm×15 cm, C2; 10 cm×10 cm, C3). The results showed that: 1) Compared with the control plot, the sediment yield of grass-cover plot (C1-C3) decreased by 64.32%, 70.31%and 69.92%, respectively. When the plant spacing of the Agropyron cristatum plants was 15 cm×15 cm, the sand reduction benefit was the largest. 2) In the control plot, the gully headcut erosion mainly included the following steps: the upstream flow incision, head wall erosion by the wall flow, plunge pool erosion by the jet flow, and finally the gully bank collapse,whereas,in the grass-covered plot,the gully headcut erosion was determined by the wall flow, plunge pool erosion by the jet flow, and the collapse of the hanging soil-root matrix at the gully head. The collapse was also the main reason for the gully headcut erosion of grassland. Alternatively, the accumulation distance of gully head in the grass-covered plot was shortened by 75.61% and 78.87%, and the accumulation distance of gully head in both plots showed a significant power function with time. 3) The longitudinal section of erosion gully in the control plot was in ladder-shaped, and the gully head was rectangular; while in the grass-covered plot was trapezoid and arc-shaped. The average incision depth of the gully channel in the grass-covered was 1.64-1.92 times as deep as that in the control plot. However, the developed area of the gully channel decreased with the increase of root density, and the area of the gully in the grass-covered plot decreased by 68.0%-74.0% compared with that in the control plot. The findings can provide a promising approach for the implementation of the gully stabilization and loess-tableland protection in the gully region of Loess Plateau in China. [ABSTRACT FROM AUTHOR]

Evolutionary innovations and ecological competition are factors often cited as drivers of adaptive diversification. Yet many innovations result in stabilizing rather than diversifying selection on morphology, and morphological disparity among coexisting species can reflect competitive exclusion (species sorting) rather than sympatric adaptive divergence (character displacement). We studied the innovation of gliding in dragons (Agamidae) and squirrels (Sciuridae) and its effect on subsequent body size diversification. We found that gliding either had no impact (squirrels) or resulted in strong stabilizing selection on body size (dragons). Despite this constraining effect in dragons, sympatric gliders exhibit greater size disparity compared with allopatric gliders, a pattern consistent with, although not exclusively explained by, ecological competition changing the adaptive landscape of body size evolution to induce character displacement. These results show that innovations do not necessarily instigate further differentiation among species, as is so often assumed, and suggest that competition can be a powerful force generating morphological divergence among coexisting species, even in the face of strong stabilizing selection. [ABSTRACT FROM AUTHOR]

Morphological stasis or the absence of morphological change is a well‐known phenomenon in the paleontological record, yet it is poorly integrated with neontological evidence. Recent evidence suggests that cryptic species complexes may remain morphologically identical due to morphological stasis. Here, we describe a case of long‐term stasis in the Stygocapitella cryptic species complex (Parergodrilidae, Orbiniida, Annelida). Using phylogenetic methods and morphological data, we find that rates of morphological evolution in Stygocapitella are significantly slower than in closely related taxa (Nerillidae, Orbiniidae). Assessment of quantitative and qualitative morphology revealed the presence of four morphotypes with only subtle differences, whereas molecular data supports 10 reproductively isolated clades. Notably, estimates for the time of Stygocapitella species divergence range from ∼275 million years to ∼18 million years, including one case of two morphologically similar species that have diverged about 140 million years ago. These findings provide evidence for morphological deceleration and long‐term morphological stasis in Stygocapitella, and that speciation is not necessarily accompanied by morphological changes. The deceleration of morphological divergence in Stygocapitella can be potentially linked to niche conservatism and tracking, coupled with the fluctuating dynamics of the interstitial environment, or genetic constraints due to progenetic evolution. Finally, we conclude that failing to integrate speciation without morphological evolution in paleontology may bias estimates of rates of speciation and morphological evolution. [ABSTRACT FROM AUTHOR]

In this study, natural illite is introduced to support nanoscale zero valent iron (NZVI). The chemical composition and the physical properties of illite supported nanoscale zero valent iron (I-NZVI) are systematically investigated, and I-NZVI is found to significantly reduce the agglomeration of the NZVI particles. A comparison of the U removal capacity between I-NZVI and NZVI over various reaction times is then conducted. With an initial concentration of U at 200 μg/L, the I-NZVI removal capacity of U is as high as 3.41 mg U/g Fe, in contrast to 2.01 mg U/g Fe by NZVI at a dosage of 0.1 g/L. The initial pH of the reaction system determines the U removal capacity of I-NZVI, since it controls the speciation of U and the electron transfer processes during the reaction. Overall, based on the comprehensive understandings of the morphological change, variations in the crystalline structure, and the valence states of U and Fe, the removal mechanisms of U by I-NZVI can be concluded as the following processes: (1) the adsorption and incorporation of U(VI) onto the surface of I-NZVI, (2) the incorporation and reduction of U(V) into Fe(II), and (3) the reduction and precipitation of U(IV) with iron. [ABSTRACT FROM AUTHOR]

It is not a new concept that marsupials and placentals are distant and distinct clades among mammals. In South America, these animals coexist, occupy similar niches and, in some cases, are similar in appearance. This is especially true with respect to the locomotor categories of smaller rodents belonging to the family Cricetidae or, more specifically, the subfamily Sigmodontinae, compared with the marsupials of the Didelphidae family. In this study, we have investigated both the similarities and the differences between the two clades by examining locomotion-dependent adaptation, a crucial survival mechanism that has affected the morphology of both clades. We applied geometric morphometrics to quantify the shape of the scapula, which is a very adaptable structure. We found similar morphological adaptations between the clades, especially with respect to adaptation to life in trees. Moreover, Didelphidae are influenced by phylogenetic history to a greater extent than Sigmodontinae with regard to variation of scapula shape and allometry. These differences can be explained by the greater degree of body size variation that exists within the Didelphidae. Didelphidae have an ancient evolutionary history in South America compared with the Sigmodontinae, which have undergone a very successful and rapid diversification more recently. [ABSTRACT FROM AUTHOR]

The study of modularity is paramount for understanding trends of phenotypic evolution, and for determining the extent to which covariation patterns are conserved across taxa and levels of biological organization. However, biologists currently lack quantitative methods for statistically comparing the strength of modular signal across datasets, and a robust approach for evaluating alternative modular hypotheses for the same dataset. As a solution to these challenges, we propose an effect size measure (ZCR) derived from the covariance ratio, and develop hypothesis‐testing procedures for their comparison. Computer simulations demonstrate that ZCR displays appropriate statistical properties and low levels of mis‐specification, implying that it correctly identifies modular signal, when present. By contrast, alternative methods based on likelihood (EMMLi) and goodness of fit (MINT) suffer from high false positive rates and high model mis‐specification rates. An empirical example in sigmodontine rodent mandibles is provided to illustrate the utility of ZCR for comparing modular hypotheses. Overall, we find that covariance ratio effect sizes are useful for comparing patterns of modular signal across datasets or for evaluating alternative modular hypotheses for the same dataset. Finally, the statistical philosophy for pairwise model comparisons using effect sizes should accommodate any future analytical developments for characterizing modular signal. [ABSTRACT FROM AUTHOR]