Experiments were carried out using acid mine drainage (AMD) from three abandoned adits that drain active mining zones of the Cerro Rico silver deposit, as well as raw municipal wastewater from the adjacent city of Potosi and locally available limestone. Alkalinity production, acidity neutralisation and metal removal were studied using cubitainers for incubation of AMD in the presence of limestone, a 1:1 mix of AMD and municipal wastewater and a 1:1 mix of AMD and wastewater in the presence of limestone. Mixing AMD with wastewater had relatively little effect on the final alkalinity achieved by limestone dissolution. Dissolved concentrations of Ag, Al, As, Cd, Cr, Fe, Pb, Sb, Se, Sn, V and Zn were reduced by AMD and wastewater incubation, with particularly efficient As removal. AMD mixed and unmixed with wastewater then incubated with limestone generally reduced REE concentrations, but Pr and Nd concentrations increased under some limestone exposures. Incubation with wastewater alone generally reduced dissolved concentrations of REE, but La, Pr, Eu and Nd concentrations increased. The results indicate that cubitainer incubations have broader application than previously exploited and that passive treatment can be expanded to target more constituents of interest than traditionally applied. Co-treatment of AMD with wastewater is a promising new application of passive treatment. Although locally sourced limestone can increase final alkalinity up to 397 mg/l as CaCO3, it is a potential source for Mn and a few other possibly undesirable elements., Experiments were carried out using acid mine drainage (AMD) from three abandoned adits that drain active mining zones of the Cerro Rico silver deposit, as well as raw municipal wastewater from the adjacent city of Potosi and locally available limestone. Alkalinity production, acidity neutralisation and metal removal were studied using cubitainers for incubation of AMD in the presence of limestone, a 1:1 mix of AMD and municipal wastewater and a 1:1 mix of AMD and wastewater in the presence of limestone. Mixing AMD with wastewater had relatively little effect on the final alkalinity achieved by limestone dissolution. Dissolved concentrations of Ag, Al, As, Cd, Cr, Fe, Pb, Sb, Se, Sn, V and Zn were reduced by AMD and wastewater incubation, with particularly efficient As removal. AMD mixed and unmixed with wastewater then incubated with limestone generally reduced REE concentrations, but Pr and Nd concentrations increased under some limestone exposures. Incubation with wastewater alone generally reduced dissolved concentrations of REE, but La, Pr, Eu and Nd concentrations increased. The results indicate that cubitainer incubations have broader application than previously exploited and that passive treatment can be expanded to target more constituents of interest than traditionally applied. Co-treatment of AMD with wastewater is a promising new application of passive treatment. Although locally sourced limestone can increase final alkalinity up to 397 mg/l as CaCO3, it is a potential source for Mn and a few other possibly undesirable elements.