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Gardens constantly change during their lifetime due to the growth and death of plants and to the effects (or lack) of management activities. The loss of garden areas, as well as the building’s collapse, can drastically impact the view and understanding of archaeological areas. The study and reconstruction of ancient lost gardens is a complex topic, and it seems that there is a lack of a general methodological approach for assessing the revitalization and valorization activities of these gardens, especially when considering the floristic features. Here, we discuss the required steps for developing the various intervention components for achieving a deep knowledge and understanding of natural, historical, and philosophical features of the place and its culture, from the past to the current conditions. Considering this knowledge, we propose a revitalization and a cultural valorization approach of the natural elements, and we discuss the limitations and issues arising in lost garden studies.

Since the nineteenth century, street trees have become a significant component of urban landscapes, with multiple ecological roles and social-economic functions. However, data about their abundance and diversity, and information about the criteria used for the species selection, are still limited. Besides providing a historical overview, we analyze and compare data on abundance, diversity and criteria of species selection of street trees in 15 Italian cities chosen considering their representa- tiveness along a bioclimatic transect across Italy. The biodiversity of urban street trees resulted quite rich with 277 species (about 60% are exotic); among these, Platanus × hispanica Mill. ex Münchh., Celtis australis L. and Quercus ilex L. resulted as the most common species. Besides the general suitability for the local climatic conditions, several factors seem to concur in the species selection, such as their aesthetic appeal and dimension. Other selection criteria based on the species’ ecology or functionality were not evident.

Identifying species involved in biodeterioration processes is helpful, however further effort is needed to assess their ecological requirements and actual activity. Black fungi (BF) represent one of the most underestimated threats to stone cultural heritage in the Mediterranean basin; they are difficult to kill or remove due to their ability to grow inside the rock and cope with several stresses. Despite this, little is known about BF and factors favoring their growth on stone surfaces. Eighteen BF species were here investigated for temperature and salt tolerance, and metabolic traits by plate assays. The relation between some highly damaged monuments and their BF settlers was assessed using X-ray diffraction analysis, mercury intrusion porosimetry, and SEM. The sensitiveness to four commonly used traditional biocides was also tested. All strains were able to grow within the range of 5–25 °C and in the presence of 3.5% NaCl. Instrumental analyses were fundamental in discovering the relation between halophilic strains and weathered marble sculptures. The acid, cellulase, esterase, and protease production recorded proved BF’s potential to produce a chemical action on carbonate stones and likely affect other materials/historical artefacts. Besides, the use of carboxymethylcellulose and Tween 20 should be evaluated in restoration practice to prevent tertiary bioreceptivity. Agar diffusion tests helped identify the most resistant species to biocides, opening the perspective of its use as reference organisms in material testing procedures.

The conservation of stone monuments is a constant concern due to their continuous weathering, in which biofouling plays a relevant role. To enhance the effectiveness of biocidal treatments and to avoid environmental issues related to their possible toxicity, this research aims at formulating and characterizing a coating charged with an eco-friendly biocide and showing hydrophobic properties. For this purpose, zosteric sodium salt—a natural biocide product—has been encapsulated into two silica nanocontainers and dispersed into a tetraethoxysilane-based (TEOS) coating also containing TiO2 nanoparticles. The coatings were applied on four different types of stone: brick, mortar, travertine, and Carrara marble. The effectiveness of the coating formulations and their compatibility concerning the properties of coated stones were assessed. The results showed that all coatings conferred a hydrophobic character to the substrate, as demonstrated by the increase of the static contact angle and the reduction in the capillary water absorption coefficient. The transmission of water vapor of the natural stones was preserved as well as their natural aspect. Furthermore, the coatings were homogeneously distributed on the surface and crack-free. Therefore, the protective capability of the coatings was successfully demonstrated.

bstract Purpose The colonisation of building material by photosynthetic organisms is highly dependent on the environmental conditions and the nature of the substrate. The growths of red-orange phototrophic biofilms have been widely reported in the literature and have commonly been associated with the order Trentepohliales, whose ecological and taxonomical information needs to be improved. Considering the recurrent presence of such biofilms throughout the Lazio region, we would identify the occurring species and define their favourable environmental conditions, through morphological, genetic and ecological analyses. Results Biofilms were collected across an altitude range of 0 to 860 m asl, occurring from the coast to 60-km inland. A dominant presence of the filamentous terrestrial green alga Trentepohlia umbrina (Kützing) Bornet was confirmed in all sites sometimes mixed with cyanobacteria of the genus Gloeocapsa. The distribution of Trentepohliales is generally given as pan-tropical, but some species, such as T. umbrina, are also distributed in temperate climates. Here, it is reported for the first time a Mediterranean occurrence of the species. Low humidity and light conditions, such as those occurring on vertical surfaces with mainly northern aspects, are the preferred environmental conditions. Coastal areas were more favourable for its growth, probably due to the higher nocturnal condensation that occurs here. Concrete and mortars were particularly bioreceptive to T. umbrina, but marble and trachyte were also colonised under favourable conditions. Conclusions The findings better define the ecological range of this species, suggesting a wider biogeographic distribution, and adding information on morphological features and genetic data.

Coupled human–environment systems and traditional agricultural landscapes can be a key element in the conservation of biodiversity, ecological functionality, and cultural heritage. Terraced landscapes are a relevant example of traditional landscapes within the Mediterranean area, but they are now threatened due to the abandonment of agricultural activities. In order to identify factors that can affect the conservation strategies needed to maintain terraced landscapes in the Mediterranean area, we performed a literature review on studies about terraces and their management, soil erosion and vegetation dynamics after abandonment, etc. We collated a total of 285 scientific papers reporting 293 case studies in 19 countries. The majority of these studies analyze dry stone terraces and their influence on soil erosion and water runoff, either in cultivated or abandoned terrace systems. Only a minority of papers suggest maintenance or conservation strategies or involve farmers, exploring their decision-making. The conservation of terraced landscapes is in the hands of local farmers, and thus they should be actively involved in decision-making to find the most suitable strategies for assuring the continuity in farming and preserve cultural landscapes in the Mediterranean area.

The conservation of underground tombs is affected by several physical-chemical and biological factors, which could be reduced by insulating systems able to maintain the microclimatic stability also decreasing the biodeterioration risk. In Mediterranean areas, wild ephemeral plants, which reduce their cover during the hot season, seem unsuitable for reducing summer overheating. In this study, we wish to assess the influence of vegetation cover and of overlaying soil, after the establishment of an evergreen turf of a cultivar of Cynodon dactylon, on two tombs in the Etruscan Necropolis of Monterozzi, covered by linear-shaped tumuli. Therefore, we evaluated for 10 months the thermo-hygrometric values of these tombs, together with two tombs as controls. We also evaluated the different tumuli’s morphologies and the related received solar radiation. Results confirmed that late summer and early autumn as critical microclimatic periods for the risk factors of hypogeal paintings when peaks of superficial temperature occur. A positive influence of vegetation cover on maintaining constant humidity and internal temperatures was detected, but the mounds orientation, as well as soil depth, seems to have a relevant role. Considering the naturalistic features of the area and the related cultural ecosystem services, a careful selection of wild plants is suggested.

Plant colonization on archaeological structures is limited by the substrate conditions of stone surfaces. Despite its relevance for ecological studies and the management plans of archaeological sites, the knowledge on monuments substrate (e.g., stones, microhabitats, microsites), which could be suitable sites for plant establishment, is an underestimated topic. Here, we aim to identify the substrate preference of plants in colonizing stone surfaces as a first step in evaluating the biodeterioration risk. We selected the Pasargadae World Heritage Site (WHS) for its variety of different conditions, and we assessed the substrate preference of 142 ruderal plants occurring on nine monuments, two types of stones, and eight microsites. Our data indicated that stone typology and microsite conditions affected species composition, diversity, and richness. The porosity of the different limestone and the progressive weathering phenomena resulted as relevant factors in plant colonization. Significantly, microsites as a junction of two stone blocks (M1a) and big fractures covered by soil (M3) hosted the most favorable conditions, which needed periodic monitoring during the maintenance activities of the site. Evaluating the substrate preference of ruderal plants can contribute to the identification of the high-risk areas for plant colonization, which need to be considered in the management plans of archaeological sites.

Plant colonization in the archaeological areas, despite its relevant role for monument conservation, has not dedicated noticeable concern. For the first time in Iran, we carried out a floristic evaluation in an archaeological area and its biodeterioration hazard, choosing Pasargadae WHS. Among the 142 detected species, found in the 9 study areas of the site, 48 ruderal common species resulted widely distributed, with a dominance of grasses (mainly Therophytes and Hemicryptophytes) belonging to the Asteraceae and Poaceae families. The analysis of plant colonization identified 10 possible Micro-Sites of growth (MS = place of growth on stone observed at small scale), half of which are described for the first time. Besides the life forms of plants, plant colonization in specific MS was selected as a parameter for hazard evaluation. Then, we coupled this information with five determinant parameters for plant growths, having each one three classes of value to obtain the final evaluation. The colonization in joints and fractures of stone blocks, as well as the surface covered by soil, represent the highest hazard conditions for biodeterioration. Green-gray sandy limestone, which was used as a platform for palaces, had the highest colonization. The collected data will contribute to the site management.

The hypogeal cultural heritage due to their favourable environmental conditions can be subject to microbial attack usually controlled using biocides. Recently the number of products allowed in the restoration practice has been reduced because, in addition to their effectiveness, the biocidal product should not have effects on the treated surfaces, and it should be safe for the operator and environment. Following this field research, we propose a reconstruction experiment in which we tested 4 different natural products (Clove oil, Oregano Oil, Usnic Acid, IPA+ DMSO + Usnic Acid) on tiles, reproducing the substrate of Etruscan hypogeal tombs (Macco) of Monterozzi Necropolis, inoculated with a selection of fungal/bacterial strains. After one month from the biocide’s application, we evaluated their efficiency by image analysis and by culture analysis. The achieved results evidenced a clear gradient between the four tested biocides and gave suggestions for further improvements.

Wall paintings have been a cultural expression of human creativity throughout history. Their degradation or destruction represents a loss to the world’s cultural heritage, and fungi have been identified as a major contributor to their decay. We provide a critical review of fungi isolated from worldwide wall paintings between 1961–2021. One-hundred three scientific papers were reviewed focusing on fungal diversity, isolation protocols, and spatial distribution of data. The study sites were grouped into five environmental categories on the basis of the expected major microclimatic conditions (temperature, relative humidity, ventilation), and the possible relationship with the species found was investigated. The highest number of records were localized in Europe, with 38 sites on a total of 74, 20 of which were from Italy. A total of 378 fungal entries were obtained, consisting of 1209 records, belonging to 260 different species and 173 genera. The accuracy level in taxa determination was highly variable among different papers analyzed. Data showed a dominance of Ascomycota, mainly of orders Eurotiales and Hypocreales probably due to their wide distribution and easily air dispersed spores and due to the possible pitfalls linked to the isolation methods, favoring rapidly growing taxa. Statistical analyses revealed that fungal communities were not strictly linked to environmental categories with different ventilation, temperature, and humidity. Such findings may be due to the wide geographical area, the wide heterogeneity of the data, and/or the absence of standardized sampling and analyses protocols. They could also be the result of the dominance of some prevailing factors in the various sites that mask the influence one of each other.

Subterranean Cultural Heritage sites are frequently subject to biological colonization due to the high levels of humidity, even in conditions of low irradiance and oligotrophy. Here microorganisms form complex communities that may be dangerous through mineral precipitation, through the softening of materials or causing frequent surface discolorations. A reduction of contamination’s sources along with the control of microclimatic conditions and biocide treatments (overall performed with benzalkonium chloride) are necessary to reduce microbial growths. Dark discolorations have been recorded in the painted Etruscan tombs of Tarquinia, two of which have been analyzed to collect taxonomical, physiological, and ecological information. Eighteen dark-pigmented fungi were isolated among a wider culturable fraction: nine from blackening areas and nine from door sealings, a possible route of contamination. Isolates belonged to three major groups: Chaetothyriales, Capnodiales (Family Cladosporiaceae), and Acremonium-like fungi. Exophiala angulospora and Cyphellophora olivacea, a novelty for hypogea, were identified, while others need further investigations as possible new taxa. The metabolic skills of the detected species showed their potential dangerousness for the materials. Their tolerance to benzalkonium chloride-based products suggested a certain favouring effect through the decreasing competitiveness of less resistant species. The type of covering of the dromos may influence the risk of outer contamination. Fungal occurrence can be favoured by root penetration.

The colonisation of stone by different organisms often leaves biodeterioration patterns (BPs) on the surfaces even if their presence is no longer detectable. Peculiar weathering patterns on monuments and rocks, such as pitting phenomena, were recognised as a source of information on past colonisers and environmental conditions. The evident inhibition areas for new bio-patinas observed on the marble blocks of the Caestia Pyramid in Rome, recognisable as tracks of previous colonisations, seem a source for developing new natural products suitable for restoration activities. To hypothesise past occurring communities and species, which gave rise to such BPs, we carried out both in situ observations and analyses of the rich historical available iconography (mainly photographs). Moreover, we analysed literature on the lichen species colonising carbonate stones used in Roman sites. Considering morphology, biochemical properties and historical data on 90 lichen species already reported in Latium archaeological sites, we suppose lichen species belonging to the genus Circinaria (Aspicilia s.l.) to be the main aetiological agent of such peculiar BPs. These results seem relevant to highlight the long-lasting allelopathic properties of some lichen substances potentially applicable as a natural product to control colonisation, improving the environmental and economical sustainability of stone restoration.

In restoration and conservation practices, biocide treatments are considered one of the most practical approaches to remove biological colonization on artworks, including stone. Numerous studies have focused on the short- and long-term effects of these treatments and recently many alternative methods to reduce their potential hazards to human health and the environment have been proposed. In this study, a solvent gel containing dimethyl sulfoxide (DMSO), already used to clean paintings, was applied on colonized marble artifacts at the monumental cemetery of Bonaria (Cagliari – Italy) to remove biological patinas. The protocol efficiency was evaluated by scanning electronic microscopy, rugosimetric and colorimetric measurements and growth tests. A comparative study also was performed to validate the method using biocides currently used in conservation. The results demonstrate that DMSO solvent gel is efficient at removing patinas on stone, of low impact, easy to use, inexpensive and can be considered a more practical alternative to biocide treatments.

The Mediterranean basin has been a refugium for relict plant taxa and native laurophyllic forests. The Latium coasts and, especially, the Antica Lavinium site, host relict forest communities, whose natural importance is enriched by their cultural value. Here, we aim at investigating the ecological framework, cultural and historical values, and management over time, of relict communities that have Laurus nobilis and Celtis australis as their priority habitats. To achieve this, we performed vegetation surveys and we conducted statistical analyses (PCA, NMDS). Among the 45 vegetation surveys, 25 were characterized by the two target species. The PCA analysis highlighted how the L. nobilis formations and the mixed formations with C. australis present some differences but are not sufficient to describe different coenosis. The comparison among similar forests in central and southern Italy confirmed the wide coenological amplitude of L. nobilis with respect to other laurophyllic species. Antica Lavinium has an overall good preservation of laurel forest formations, but also of mixed formation with C. australis. In the area, historical, cultural, and natural characteristics mutually contributed to the development of human civilizations and plant communities, highlighting their deep linkage.

In the last decade, worldwide research has focused on innovative natural biocides and the development of organic and inorganic nanomaterials for long-lasting reliability. In this work, the biocide effects of two different biocides encapsulated in two different silica nanosystems for a multifunctional coating have been performed through in vitro tests, by using Chlorococcum sp. as a common stone biodeteriogen. Zosteric sodium salt (ZS), a green biocide, was compared with the commercial biocide, 2-mercaptobenzothiazole (MBT), widely used in the treatment of cultural heritage. The analyzed systems are the following: silica nanocapsules (NC) and silica nanoparticles (MNP) not loaded with biocides, two nanosystems loaded with ZS and MBT, and free biocides. The qualitative and quantitative evaluations of biocide efficiency were performed periodically, analyzing pigment autofluorescence to discriminate between active and inactive/dead cells. The analyses showed multiple differences. All the nanocontainers presented an initial reduction in chlorophyll’s autofluorescence. For the free biocide, the results highlighted higher efficiency for MBT than ZS. Finally, the nanosystems loaded with the different biocides highlighted a higher activity for nanocontainers loaded with the commercial biocide than the green product, and better efficiency for MNP in comparison with NC.

The forest vegetation of the southern sector of the Monti Lattari complex (Amalfi Coast) has been investigated due to the lack of information on vegetation within an area of worldwide relevance and with a high floristic richness. This area shows several environmental peculiarities due to the marked orography, with pedologic features varying from carbonate to volcanic character, and bioclimatic conditions varying in a relatively short space from Mediterranean to temperate types. The study involved an extensive phytosociological survey and the analyses revealed clear differences among the forest communities, whose syntaxonomical queries and the biogeographic and ecological characters have been discussed. The communities varied from the coastal sclerophyllous forests (Cyclamino repandi–Quercetum ilicis) to the mixed thermophilous deciduous forests (Festuco exaltatae–Aceretum neapolitani, Seslerio autumnalis–Aceretum obtusati), and from the mountain neutral–subacid forests (Salvio glutinosae–Alnetum cordatae, Castanea sativa groupment, Asperulo taurinae–Alnetum cordatae) to the mountain beech forest (Anemono apenninae–Fagetum sylvaticae). Multivariate analysis showed the high relevance of steepness, elevation and pyroclastic presence, and a low relevance of aspect in the discrimination of forest communities. Syntaxonomical interpretations showed the affinity of the local communities with those of the southern Italy, but also some interesting peculiarity. Data highlight the area as a geographical boundary and phytogeographic hinge between central and southern Italy.

A study was carried out on biodeterioration processes at six Jewish cemeteries in north-eastern Italy, which were generally subject to very poor maintenance. Several crustose lichens, as well as ruderal wooden plants, were found growing differentially on stone types in the various cemeteries. Lichens colonizing gravestones and woody plants were examined through field observations and several microscopic analyses. Evaluations were carried out on selected species and some damage risk indices were applied. The Index of Lichen Potential Biodeteriogenic Activity (LPBA) was calculated for Nanto stone, which suffered the highest degree of deterioration. The Hazard Index (HI), which is independent from the lithotype, was calculated for all the wooden plants. We also analyzed the ecological relationships of such colonization in order to evaluate indirect conservation treatments. The various lichen species were causing differential interactions with the stone, sometimes contributing in cracking and detachment of fragments. Their potential aggressiveness is mainly attributable to the different lithotypes, but also to changes in maintenance, tree canopy cover, and eutrophication. We have made suggestions for an appropriate plant management that considers their environmental, ornamental and symbolic importance in such context. For the stone conservative treatments against lichens, we also carried out tests on selected biocides.

When the Local Cuisine Still Incorporates Wild Food Plants: The Unknown Traditions of the Monti Picentini Regional Park (Southern Italy). Wild food plants are still part of the culinary traditions of many rural areas in the Mediterranean. This study aims to document these traditions and the food uses of wild plants in an area of the Monti Picentini Regional Park. We performed an ethnobotanical survey between 2013 and 2015 using standard ethnographic methods. We analyzed the gathered data using ethnobotanical indices, and we compared our results with relevant literature pertaining to uses of wild food plants in Central and Southern Italy. We interviewed 64 key informants, whose ages ranged between 40 and 93 years, and we gathered about 1900 use citations about the traditional food uses of 84 wild plant species. Many of these species are still incorporated into traditional dishes. We recorded the previously unknown food use of four plant species (Rubus hirtus Waldst. & Kit.; Petasites hybridus [L.] G. Gaertn., B. Mey. & Scherb.; Asphodelus macrocarpus Parl.; and Oenanthe fistulosa L.). We also highlighted several rare food uses as well as many other uses that are common across Central and Southern Italy. Knowledge concerning the uses of wild food plants is part of Italy’s history and culture, and it should be preserved before modernization of lifestyles and homogenization of food habits extinguish this realm of food and culinary knowledge.

The use of traditional biocides to reduce bio-deterioration phenomena of stone monuments is increasingly deterred, due to risks for human health and the environment, as well as for potential interference with materials. Extensive research is now ongoing, aiming to find alternative and eco-friendly substances or methods to halt or reduce bio-deterioration. Here we aim to provide an assessment of the results of scientific tests, in order to evaluate the most promising substances, likewise possible deficiencies and future directions in research. We performed an extensive literature review on natural biocides for controlling bio-deterioration of stone using peer-reviewed articles between 1986 and 2018. The dataset created displays information about the experimentation of a total of 61 natural substances, mostly essential oils (23), or substances of plant origin and other compounds. The methods of application of such biocides were diverse, but the in vitro tests were the prevailing ones. The most tested organisms were fungi, followed by cyanobacteria and algae. The efficacy of the substances resulted highly variable, as did protocols and experimental doses, resulting in a lack of a robust and coherent assessment of best practices. Also, we found a few papers discussing the interference of these substances with the substrate. We believe that this area of research is very promising and necessary, although additional tests with a standardised methodology are still needed.

Wild and semi-wild plants are factual resources for a local community when they satisfy its needs. According to the bio-cultural approach, these plants and associated knowledge help define the cultural identity of each community, and ethnobotanical plants constitute a particular facet of the cultural relationships between people and nature. By referring to the concept of Cultural Keystone Species (CKS), a group of species considered in the same way within a community represents a homogeneous bio- cultural trait. We tested the hypothesis that the CKS model and the related index, the Identified Cultural Importance (ICI) of species, could be useful tools to culturally define and describe groups of species as bio-cultural traits. As a dataset to test this hypothesis we considered the wild and semi-wild plants used for food and nutraceutical purposes in 13 Aga villages in Bali. Data were collected through an ethnobotanical study in 2014. A multivariate analysis method based on the Fuzzy Set Theory was used to perform quantitative analyses to find clusters of plants. The Graph Theory was instead applied in order to detect trajectories and similarity gradients in the system of groups of species. The results confirmed that groups of species can be considered as bio- cultural traits, spreading within a cultural area in different ways and conveying information about their relationship with the native culture. The ICI index and CKS concept helped us to interpret the bio-cultural traits in terms of their cultural salience, considering them as general descriptors of the bio-cultural system of a community according to bio-cultural diversity and Traditional Ecological Knowledge. In the case of the Bali Aga villages, the partition of species showed 11 groups, and several species resulted of relevant cultural importance. Among them, Arenga pinnata (Wurmb) Merr. can be considered a CKS.

Vegetation management of archaeological sites is a recurrent problem when considering the conservation of historic structures and biodiversity. In the UNESCO site of the Etruscan necropolis “La Banditaccia” (Cerveteri, Italy), several plants grow on the ancient tumuli, which are subjected to different management practices. In order to assess the influence of interventions on plant colonisation, a comparative analysis of several tumuli was performed, through structural, chorological, phytosociological and statistical evaluations. This investigation identified and defined an ecological gradient among the tumuli. The cover values of Therophytes and Hemicryptophytes decreased by half with regular cutting, while those of Geophytes and Phanerophytes increased. Mesophilic field plants (MFP), both ephemeral and perennial, represented the most abundant group. Mediterranean bushes and forests (MBF) showed a positive trend from the entrance to the last tumuli of the necropolis. This trend was clearly defined by PCA, and it seems community changes were caused by the absence of mowing activities and to a lower tourist frequentation. SIMPER analysis highlighted which species on each tumulus mainly influence their dissimilarity. This knowledge, when combined with the evaluation of the potential risk of the different plant communities growing on the tumuli, can help to identify the best management practices.

In April 2016, William Kentridge realized the bio-artwork “Triumphs and Laments” along the Tiber embankments, representing a figures procession, chosen as relevant steps of Roman history. The drawing rose in negative after applying a stencil onto the homogeneous black biopatinas and removing them through a low-pressure washer. The patinas, growing on the travertine surfaces, were dominated by cyanobacteria communities, mainly composed by Chroococcus lithophilus, Myxosarcina spectabilis, Tolypothrix byssoidea, and Synechocistis pevalekii. To collect precise data on the dynamic of biological recolonization, we monitored the figure's life by 2016–2020, analyzing the progressive darkening, which gave rise to the artwork disappearance. By image analysis and color variation values (ΔE), using a detailed photographic survey (every 3–4 months) and colorimetric sampling (once in the year), we assessed the recolonization phenomena and their seasonal dynamics. A progressive relevant increase of black intensity started from the 3rd year and usually 4–5 years were sufficient for a complete recolonization. We also collected climatic data and performed elaborations on Cold and Drought Stress, which provided evident correlation among biological colonization and climatic data, with their seasonal fluctuations. When a longer duration of such bio-artwork would be required, such information will be useful in suggesting treatments.

Ethnopharmacological relevance Neem (Azadirachta indica; Meliaceae) is widely known for its cold pressed seed oil, mainly used as insecticide, but also for cosmetic, medicinal and agricultural uses. The seed oil is widely employed in the Indian subcontinent, and the leaves seem to have a lower relevance, but the ethnobotanical information of Bali (Indonesia) considers the utilisation of leaves for medicinal properties. Aim of the study We report ethnopharmacological information about current uses of neem, in particular of the leaves, besides the insecticidal one, we discuss on the historical background of their uses. Materials and methods Ethnobotanical data were collected using both literature and scientific references and semi-structured interviews with 50 informants (ages ranged between 14 and 76 years old) through the snowball method in thirteen aga (indigenous Balinese) villages, following Ethic code procedures. The informants were asked to specify: which part of the plant was used, and how that plant part was used. Plant specimens were collected, identified and made into herbarium voucher. In consideration of the high variability and complex chemical constituent of neem, a HPTLC analysis of neem leaves coming from both the Indonesian island of Bali and the Indian subcontinent was carried out. Results The data on the medical use of traditional preparations from leaves of neem display a wide spectrum of applications. In the Indian subcontinent, neem leaves are used to treat dental and gastrointestinal disorders, malaria fevers, skin diseases, and as insects repellent, while the Balinese used neem leaves as a diuretic and for diabetes, headache, heartburn, and stimulating the appetite. Differences in utilisation cannot be related to chemical differences and other constituents besides limonoids must be investigated and related to the multipurpose activity of neem. Conclusion This study revealed that neem leaves are believed to treat diabetes in both Balinese and Indian communities. Limonoids can not be considered the only responsible of digestive properties. Further research would be the validation of this report by enzymatic tests and the identification of active constituents.

Pink patinas and rosy discolorations on stones and mural paintings are often of biological origin, although they are frequently ascribed to chemical causes. This work represents the first report of this kind of biodeterioration in Pompeii, where we found a pink dusty patina in the “Terme del Foro”. Non-invasive sampling was performed to identify the aetiology of the discoloration. Interestingly, the pink pigmented bacterial species Arthrobacter agilis was found associated to the patina, pointing to a role of this bacterial species in rosy discoloration. Both morphology of the isolate and 16S rDNA analysis were consistent with identification as A. agilis. Raman spectroscopy was carried out to compare the pigments from the pink patina with those associated with A. agilis isolates, and the reference strain A. agilis DSM 20550. A spectroscopically identical carotenoid-like pigment was identified in all the three types of sample, arguing for a biological origin of the patina. Observations of pink patina distribution suggest that low-lighting and salinity are likely to play a role in patina development.

The archaeological site of Herculaneum (Campania, Italy), which was buried as a result of the eruption of Mt. Vesuvius in the first Century C.E., was first excavated in the XVIII century. It has been included on the UNESCO World Heritage List since 1997, and in 2001 the Herculaneum Conservation Project (HCP) was started, which has carried out, among other activities, studies and conservation interventions across the site. Up until this time, little data has been available on the growth of biological agents that could cause biodeterioration of wall paintings and archaeological structures. Particularly, the presence of rosy discoloration is frequent on ancient monuments of the Vesuvian area, even if such phenomenon has so far been largely neglected. In this study, we describe, for the first time, the pink patina distribution and the microbial species isolated from the House of the Bicentenary in Herculaneum. By combining culture-based approaches with molecular and phylogenetic analyses we reliably isolated the pink-producing bacterial species and attributed them primarily to Arthrobacter agilis, and secondarily to Rhodococcus corynebacterioides, and Methylobacterium extorquens. Strains closely related to Dietzia maris and Gordonia rubripertincta were also isolated. With the exception of M. extorquens, a proteobacterium, all of the other isolates belong to the phylum Actinobacteria. All isolates produced carotenoid pigments, suggesting that they can participate in the development of such peculiar coloration. Our data indicate the presence of a large number of pink-pigmented bacterial species in the community, even if the presence of bacteria in viable but not-culturable state, such as Rubrobacter radiotolerans, is not excludible. The culture-based approach had the advantage of (i) obtaining bacterial isolates, (ii) showing their differential ability to produce pink discolorations, and (iii) testing conditions for in vitro growth. Moreover, field observations showed an association of pink patinas with dry conditions, saline efflorescence and moderate solar radiation. Some seasonal variations were also detected, with an increase in late spring and summer.

Archaeological sites can represent a refuge for plant and animal species, protecting them from the pressures of urbanisation. However, sometimes these environmental resources can lead to negative consequences for the monuments. In order to investigate such contrasting effects, we selected the UNESCO World Heritage Site Etruscan necropolis known as “La Banditaccia” (Cerveteri, Italy). We performed floristic and phytosociological analyses, and observations on roots interactions with monuments on several tombs (9 tumuli and 5 cube-shaped tombs) and applied commonly used indices to evaluate damages to the structures. The study revealed rich biodiversity of the site and the presence of rare plants, but also of some invasive species. The analysis of the phytosociological sets showed differences among vegetation types found on the tumuli as a function of the varying degrees of anthropization. Abundant plant colonisation was detected on the top of the tumuli, sometimes causing damages to the structures. The results of the Impact Index analysis showed that the dynamic evolution of vegetation constitutes a significant risk to the archaeological structures. We suggest that careful management of vegetation should achieve equilibrium between the management requirements for the monuments and conservation of naturalistic values. A deeper evaluation of the interactions between plants and monuments is however necessary.

Stone surfaces can be subjected to biodeterioration due to the growth of photosynthetic organisms where favourable conditions of the environment and specific substrate materials are present. In this work, we investigated through genetic analysis and ecological observations the widely occurring reddish patinas, which are spread on stones in particular environmental conditions. We collected patina occurrence and environmental data at sites in the Lazio region covering an altitude range of 0 to 650 m asl and distances up to 70 km from the coast to define the ecological range. The results of blast analysis of DNA sequences confirmed a dominant presence of the filamentous terrestrial green alga Trentepohlia umbrina (Kützing) Bornet in all sites, sometimes mixed to cyanobacteria of the genus Gloeocapsa. In one site we identified T. aurea (L.) C. Martius, which occurs in different micro-environmental conditions. Originally the distribution of Trentepohliales was given as pan-tropical, but some temperate taxa have now been identified, such as T. umbrina. Preferred environmental conditions of this species were a relatively low humidity, vertical (or near vertical) surfaces, temporal diurnal shade-exposure and a mainly northern aspect. Coastal areas were more favourable for its growth, as the patinas were much more developed compared to sites further inland. Cement materials and mortars resulted particularly bioreceptive, possibly due to their soluble salt content, but tuffs and marble were also colonised under favourable conditions. The findings here suggest that the ecological preference of this species is wider than previously thought and urges the need for detailed studies on its biodeteriogenic nature.

The Uzumlu Church is an unusual soft tuff formation located in central Turkey (Cappadocia) dated around the 7th century. The rocks on its external surfaces show a severe erosion with an evident surface detachment. Moreover, the widely distributed black-grey crusts mainly consisting of lichens point out the need of evaluating their deteriorative effects. This research was focused on lichen deterioration by indicating the biodeterioration processes and on selecting the most appropriate preservation treatments suitable for stone conservation. The interaction of lichens with stone was studied by evaluating through microscopic analyses, the spread and the depth of fungal hyphae penetration, and by applying the LPBA Index. The SEM analysis of all fragments shows a dramatic loss of the stone matrix and a dense network of fungal hyphae within the rock. Nevertheless, both the high penetration of fungal hyphae, and the consequent difficulty in their removal without a strong peeling effect, both the relevant loss of the stone matrix and finally the evident reduction of water penetration when crusts are present in the surfaces, advise against their removal. Further chemical treatments of consolidation should be carefully evaluated.

In this study, we support the hypothesis that wall plant species come mainly from rocky habitats, taking into account that ancient walls show similar characteristics to rocks. The occurrence of wall species in natural habitats was investigated. The main literature concerning wall and natural vegetation of central-southern Italy was viewed. Vegetation synoptic tables were analysed through statistical procedures for comparing occurrence and behaviour of wall species in ruderal habitats as archaeological sites and natural ones.The study pointed out that wall species show similar ecological and coenological features in both habitats. This confirms that the main natural habitat from which wall species come from are rocks, but secondarily also ephemeral Mediterranean meadows and garrigues. Particularly, some wall species belonging to Parietarietea judaicae class are participating in the formation of natural rocky communities of Asplenietea trichomanis. Species occurring on emerging ruins form ephemeral meadows belonging to Helianthemetea guttati, also found in natural Mediterranean environment. On wide tops of ruins, where community dynamism is higher, some perennial herbaceous and shrubby species participate in the formation of impoverished communities matching to natural aspects of Mediterranean maquis. The knowledge of the natural habitat of wall species can be useful for a better management of archaeological sites.

Biodeterioration phenomena are of great relevance in rock settlements, due to favourable environmental conditions, such as the infiltration of rainwaters, condensation phenomena and abundance of salts and organic nutrients. Rinaldi’s rock shelter in Filiano, which is located in a natural forest of mixed oaks is of great value due to the important traces of prehistoric paintings. It is an emblematic case of the delicate balance, achieved throughout the centuries, between the environment and artwork. During the plurimillenarian history of the site, a portion of the ceiling that covered the shelter collapsed, leaving signs that are still visible today, together with traces of blackening left by the fires of ancient settlements. Several of the biodeteriogens typical of rocky habitats have already been detected and include algae, cyanobacteria, mosses, lichens, vascular plants and fungi, which form macroscopic communities. Each community has an ecological preference and the mapping of their distribution is a suitable tool for understanding variations in the environmental factors that most affect them. Relating ecological data to the taxonomical characterization of the species and to the spatial distribution of each community, a site map of the humidity and of the nutrients was obtained. Among the various communities, microcolonial fungi (MCF), which appear as little black spots, here, represent the most critical risk factor, due to their low water needs. An evaluation of the biological risk for the possible future attack of such a biological community was made, suggesting indirect mitigation measures, through modification of the microclimatic and local ventilation conditions., Conservation Science in Cultural Heritage, Vol 15, No 2 (2015): Special Issue for Second Conference on Biology and Archaeobiology – from Knowledge to Preventive Conservation

The island of Bali has several aga (indigenous) villages that have survived despite the pressures of an intense tourist industry and agricultural changes. A rich ethnobotanical culture persists, but the meaning of differences in traditional ethnobotanical knowledge (TEK) remains under-explored. We analyzed information obtained from interviews of inhabitants from diverse villages on food and nutraceutical plants to identify plant patterns, i.e., relevant plant groups with species sharing a similar occurrence. Through cluster analysis, we identified 12 main groups of species and found that species were grouped based on traditional knowledge and the use each community made of plants on the whole, and not on growth forms nor on specific uses. The frequency distribution of species clusters showed a bimodal trend, with several groups present only in few villages, and a few groups present in almost all villages. The latter are defined as “core groups,” and represent the shared TEK of each aga community. Other “satellite species groups” embodied in the local TEK were related to small isolated communities. Cultural erosion caused by modernization, with the consequent fragmentation of information, was judged to be one of the main causes of increasing TEK heterogeneity.

The island of Bali has several traditional Aga villages that survive under the pressures of an intense tourist industry and agricultural changes. In order to understand possible impacts on traditional ethnobotanical knowledge (TEK) in Bali, we interviewed local people living in 13 traditional villages regarding the number of known plants and their uses. We analyzed socioeconomic factors influencing change of such knowledge at both individual (informant) and community (village) level. We identified a total of 149 food and nutraceutical plants being used in the study area. Neither gender, occupation, income, nor level of formal education had a significant effect on TEK. However, informant’s age and village status were found to play an important role in the retention of TEK at an individual level. At the village level, the use of Internet/smart phones was an important predictor of cultural erosion.

Terraces are traditional engineered ecosystems that affect the hydro-geological equilibrium, slope stability, and local communities. The aims of this paper are (i) iden- tifying environmental factors that affect terrace stability in the Amalfi Coast, (ii) defining agriculturalists’ observations on environmental changes within that system and (iii) exploring potentiality of these observations to better define conservation strategies. All available data on physical and ecological factors recognized to affect the terrace system were collected and analyzed. Interviews were conducted with agriculturalists to obtain long-term observations on environmental factors that interact with this system. Land- slides are more frequent where rainfall is high and during winter. Fires have an uneven annual distribution, with higher frequency during summers. Agriculturalists detailed com- plex interactions among environmental factors, economic elements, and terraces. These observations represent a valuable resource for defining causes and effects of aban- donment and for better addressing conservation strategies.

The need of planning more sustainable cities leads to a wider use of extensive green roofs (EGRs) as they provide significant advantages to the urban environment (e.g., energy conservation and increase of biodiversity). In Central and North Europe, as well as in North America and Asia, EGRs are generally included in new building designs, whereas they are still uncommon in Mediterranean countries. The adaptations of many Mediterranean plants to drought stress and their floristic diversity constitute, however, positive elements in finding solutions for them. This research proposes a methodological approach to select wild species for EGRs based on ecological characteristics (using natural ecosystems as templates for green roof design). An extensive bibliographic search on plants proposed for EGRs in Mediterranean countries has led to the creation of a wide database. Other plants were selected considering their synecological, structural, and autoecological characteristics. All the data were integrated in a comprehensive database of 138 taxa potentially suitable for setting EGRs according to their syntaxonomical classification and their ecological behavior (fitting both the Mediterranean and EGR environmental conditions). The selected taxa could enlarge the pool of species for EGRs in Mediterranean cities, increasing urban biodiversity.

A wide sampling for isolating highly destructive and extremotolerant black fungi was performed from Italian monuments in selected historical sites which include the “Cortile della Pigna”, Vatican Museum and the St Peter colonnade (Vatican City State), the monumental cemetery of Bonaria (Cagliari), and other monuments in the city of Cagliari. Thirty out of seventy-four strains isolated were identified basing on ITS Blastn comparison. Based on multilocus phylogeny and morphological data, one new genus and species Lithophila guttulata, five new species Knufia marmoricola, K. vaticanii, K. karalitana, K. mediterranea and Exophiala bonariae, order Chaetothyriales and one new genus and species, Saxophila tyrrhenica, and two new species Vermiconia calcicola and Devriesia sardiniae, order Capnodiales, were proposed. Ecological considerations are put forward.

Defining and understanding the long-term social and ecological evolution of rural cultural landscapes can provide insights into complex dynamics of landscape and environment changes. Land cover changes (LCCs) in Mediterranean-type ecosystems are mainly due to human-induced landscape transformations. Multi-scale spatial analysis can provide useful information in the interpretation of LCCs data and contribute to identifying underlying drivers of landscape change. In the present study, we analyze eight diachronic land cover maps and perform statistical data assessments of human pressure in the Tolfa–Cerite district (Northern Latium, central Italy) to investigate potential changes in the cultural landscape. The Tolfa– Cerite district is a generally dry area with subhumid–humid sites and an interesting mosaic of Mediterranean-temperate vegetation, agricultural and pastoral land, and a millenarian human presence. LCCs were assessed over a period of 57 years (1949–2006) using maps at both low- resolution (1:100.000) and high-resolution (1:25.000) with different class nomenclature sys- tems. Three primary land cover changes have been observed during the investigated period: (i) urbanization, (ii) land abandonment, and (iii) deforestation. While the former two classes of landscape change are particularly common in the northern Mediterranean region, forest conversion to pastures and shrub lands due to intensive grazing, fires, climate aridity, and increasing human pressure is, nowadays, rarely observed in Italy. Better understanding the influence of population dynamics at the local scale and other drivers of LCCs can help fine- tuning conservation policies looking at landscape quality, diversity, and fragmentation.

Ivy (Hedera helix L.) growing on monuments is a common issue in the study of nature’s relationship with artefacts in the Euro-Mediterranean area. This is due to its pervasiveness and its double role as protective and as deteriogen. We studied the effect of ivy colonization within the archaeological site of Villa of Massentius. We applied a quantitative method derived from monument mapping method by Fitzner, to evaluate deterioration due to ivy colonization on vertical surfaces. Instead, the capping ivy effects was investigated through a multi-parameter evaluation. We recognized three different damage categories: the superficial loss of surface (W), ivy residues on wall after the removal event (I), and the loss of compact stone fragments (P/O). Mean W corresponded to 58% and P/O to 14%. The total damage rate was positively correlated to the amount of ivy cover and to favourable shady conditions. For the capping ivy, we calculated an average loss of 4.34 cm3/m2 and a high level of soil deposit, which led to a secondary ivy colonization process. This paper proposes a contribution to the identification and quantification of ivy deteriogen activity. The application of the proposed method also provides a useful tool for planning restoration activity.

The delineation of bioclimatic areas is a key tool in informing sustainable land management strategies, especially in the light of climate change. It requires an in-depth analysis of climate data but also of the physical and biological factors that affect the distribution of natural vegetation. The present study applies an exploratory data analysis aimed at defining bioclimatic conditions in an area characterized by steep slopes and prone to soil erosion (Amalfi Coast, southern Italy). To this end, we analyzed long-term (1959–1999) monthly rainfall and temperature data measured at 28 gauging stations within the study area using a geographic information system and multivariate statistical tools. Then, we analyzed how the spatial distribution of rainfall and temperature data relates to topography, vegetation cover, and soil erosion. We also derived bioclimatic indicators from the geostatistical analysis. We then identify and comment on drawbacks found in the procedure used in the present study, discussing possible solutions to overcome such problems. We finally discuss the potential contribution of this study to the development of sustainable land management policies for socio-ecological systems with complex topography.

Despite the wide spectrum of literature on stone biodeterioration associated with pitting, there has not been sufficient description of some general topics related to stone biopitting in terrestrial conditions, nor of the taxonomy of the organisms supporting it. In order to synthesize the information available in the literature, and to give a critical analysis of the bibliographic data, an interactive on-line database has been created. Among the about 800 papers selected in the first step of creating this database. Only 24 studies reported on biopitting, giving information on the object, material composition and associated organisms. These first data concern 83 different sites, for a total of 249 samples, coming mainly from the Mediterranean bioclimatic area, even though the biopitting phenomenon is not exclusive to this climate. The most commonly occurring organisms are cyanobacteria, and the associated environmental conditions are dryness, arising from various factors, such as the low porosity of the stone; the exposure conditions; and the bioclimate. These factors explain very high appearance of organisms and especially cyanobacteria in marble, their preference for vertical or subvertical surfaces, and their high occurrence in Mediterranean and desert climate. The lack of information describing the entire phenomenon i.e., type of stone, exposure conditions, and all biodeteriogens present, doesn't permit the full use of the database's interactive potential.

This study analyses the plant colonization of the stone embankments along the Tiber River (the Lungotevere) in relation to changes of lithotype (travertine, tuff, cement), inclination (80°, 60°, 45°), stone size, and exposure. The surveys of plant communities were carried out according to the phytosociological method and considering the variability of the ecological conditions. The vegetation data were analyzed using multivariate statistical analysis (cluster analysis) in order to highlight the affinity among the various groups of sampling areas. The ecological features of the vegetation, according to Ellenberg indicator values, were considered for the ecological interpretation. Results showed that many environmental factors interact in giving rise to a differential plant cover and species richness, but that some are more relevant than others, such as the lithotype nature, especially in relation to the differential stone porosity. Also, changes in inclination of the walls cause variations in plant colonization, especially in the case of travertine and less in the case of tuff, as a consequence of the differing porosity of these materials. The study also confirms the subordinate role of exposure with respect to other factors.