251. Cloning of two hypothalamic cDNAs encoding tissue-specific transcripts in the preoptic area and testis.
- Author
-
Nowak FV
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, DNA isolation & purification, Male, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Messenger analysis, Rats, Rats, Inbred Strains, Regulatory Sequences, Nucleic Acid, Transcription, Genetic, Cloning, Molecular, DNA genetics, Gonadotropin-Releasing Hormone genetics, Hypothalamus chemistry, Preoptic Area chemistry, Testis chemistry
- Abstract
Neuropeptides are central to the regulation of normal sexual development and reproduction. Two new hypothalamic cDNAs have been identified by Northern blot analysis and molecular cloning. Each potentially encodes a precursor for a unique GnRH-like decapeptide. Northern blot analysis reveals tissue-specific transcripts from each gene in the hypothalamus and testis. These preoptic area regulatory factor genes, PORF-1 and PORF-2, may thus encode members of a family of GnRH-related peptides which have been described in these tissues.
- Published
- 1990
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