275 results on '"da Silva, Carlos Alberto"'
Search Results
252. Influence of Mineral Particle Films and Biomaterials on Guava Fruits and Implications for the Oviposition of Anastrepha obliqua (Macquart) (Diptera: Tephritidae).
- Author
-
da Costa, Daniela Ribeiro, Leite, Suzany Aguiar, dos Santos, Mateus Pereira, Coelho, Beatriz Sousa, Moreira, Aldenise Alves, Domingues da Silva, Carlos Alberto, Joachim-Bravo, Iara Sordi, Castellani, Maria Aparecida, and Stelinski, Lukasz L.
- Subjects
GUAVA ,TROPICAL fruit ,FRUIT ,ANASTREPHA ,TEPHRITIDAE ,OVIPARITY - Abstract
Simple Summary: Among the main phytosanitary problems that affect the production and commercialization of fresh fruits, the occurrence of fruit flies (Diptera: Tephritidae) is one of the main obstacles. The control of these tephritids is mainly performed through the use of toxic baits. The use of mineral films and biomaterials may constitute a viable alternative in relation to the traditional insecticide method, mainly because they do not contaminate the environment and do not leave toxic residues harmful to humans and animals in treated products. Therefore, by modifying the color and texture of the fruit cuticule that covers the plant tissues, kaolin affects the perception of arthropod pests, impairing the localization process and acceptance of the host plant and, consequently, its feeding and oviposition. In this study, we hypothesized that the color changes of guava fruits because of mineral particle films and biomaterials can affect the oviposition of fruit flies. The results obtained are promising and show that mineral films and biomaterials interfering with the color of guavas inhibited the oviposition of A. obliqua. Therefore, they can be used to protect guava fruits from the damage caused by this pest. Anastrepha obliqua (Macquart, 1835) is an important pest of tropical fruits, especially Anacardiaceae and Myrtaceae, in the Americas. The objective of this study was to evaluate the influence of mineral films and biomaterials on the coloring of guava fruits (Psidium guajava L.) and implications for the oviposition of A. obliqua. Before the bioassays, color, firmness characteristics, total soluble solids, pH, and titratable acidity were determined to characterize the maturation stage of the fruits. Pieces of guava fruit covered in aluminum foil were immersed in suspensions of mineral particles (Surround
® WP kaolin; kaolins 605, 607, 608, and 611; and talc) and biomaterials (chitosan, cassava and potato starch, and guar gum) and distilled water (control). After drying, the fruits were exposed to two A. obliqua pairs for 48 h in choice and non-choice tests, and the numbers of eggs per fruit were counted. Mineral films (Surround® WP kaolin, and kaolins 605, 607, 608, and 611) and biomaterials (cassava and potato starch) interfered with the color of guava (luminosity, chroma, and hue angle), inhibiting the oviposition of A. obliqua. Talc, chitosan, and guar gum did not influence the oviposition of A. obliqua in guava. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
253. Institutional Foundations of Management of Natural Disasters: Lessons from the Recent Cyclones in Mozambique
- Author
-
Leite da Silva, Carlos Alberto, Oliveira da Silva, Eduardo Sol, Vilar-Lopes, Gills, John, Anna, Vicente, Gil Jose, Leite da Silva, Carlos Alberto, Oliveira da Silva, Eduardo Sol, Vilar-Lopes, Gills, John, Anna, and Vicente, Gil Jose
254. Institutional Foundations of Management of Natural Disasters: Lessons from the Recent Cyclones in Mozambique
- Author
-
Leite da Silva, Carlos Alberto, Oliveira da Silva, Eduardo Sol, Vilar-Lopes, Gills, John, Anna, Vicente, Gil Jose, Leite da Silva, Carlos Alberto, Oliveira da Silva, Eduardo Sol, Vilar-Lopes, Gills, John, Anna, and Vicente, Gil Jose
255. Exposição ocupacional a névoas ácidas e alterações salivares
- Author
-
Lima da Silva, Carlos Alberto, primary
- Published
- 1970
- Full Text
- View/download PDF
256. Efeito do excesso de peso sobre parâmetros espirométricos de adolescentes submetidos ao exercício.
- Author
-
De Oliveira Costa, Rayana, Pereira Silva, Juliana, Mattos Lacerda, Eliana, Dias, Rodrigo, Alexandre Pezolato, Vitor, Da Silva, Carlos Alberto, Krinski, Kleverton, De Valois Correia Junior, Marco Aurélio, and Cieslak, Fabrício
- Abstract
Objective: To evaluate effects of overweight on spirometric parameters in adolescents who underwent bronchial provocation test for exercise. Methods: We included 71 male adolescents. The diagnosis of asthma was done based on participants' clinical history and on the International Study Questionnaire Asthma and Allergies in Childhood, and the diagnosis of obesity was based on body mass index above 95th percentile. The bronchospasm induced by exercise was assessed using the run-walk test on a treadmill for eight minutes. The decrease in forced expiratory volume in one second > or equal to 10% before exercise was considered positive, and to calculate the intensity in exerciseinduced bronchospasm we measured the maximum percentage of forced expiratory volume in one second and above the curve area. Data analysis was carried out using the Mann-Whitney U test and Friedman test (ANOVA), followed by Wilcoxon test (p<0.05). In addition, we used Fisher's exact test to analyze the exercise-induced bronchospasm frequency. Results: Significant differences were observed among obese adolescents in exercise-induced bronchospasm frequency (p=0,013) and in relation to time required for recovery after exercise (p=0,007). Conclusion: Overweight can influence the increase in the exercise-induced bronchospasm frequency in non-asthmatic adolescents compared with eutrophic adolescents. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
257. Institutional Foundations of Management of Natural Disasters: Lessons from the Recent Cyclones in Mozambique
- Author
-
John, Anna, Vicente, Gil Jose, Leite da Silva, Carlos Alberto, Oliveira da Silva, Eduardo Sol, and Vilar-Lopes, Gills
- Published
- 2020
258. Oxidation of dibenzothiophene (DBT) by Serratia marcescens UCP 1549 formed biphenyl as final product
- Author
-
de Araújo Hélvia W, de Freitas Siva Marta, Lins Clarissa I, do Nascimento Aline, da Silva Carlos Alberto, and Campos-Takaki Galba M
- Subjects
Fuel ,TP315-360 ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Background The desulphurization of dibenzothiophene (DBT), a recalcitrant thiophenic fossil fuel component by Serratia marcescens (UCP 1549) in order for reducing the Sulphur content was investigated. The Study was carried out establishing the growth profile using Luria Bertani medium to different concentrations of DBT during 120 hours at 28°C, and orbital Shaker at 150 rpm. Results The results indicated that concentrations of DBT 0.5, 1.0 and 2.0 mM do not affected the growth of the bacterium. The DBT showed similar Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MCB) (3.68 mM). The desulphurization of DBT by S. marcescens was used with 96 hours of growth on 2 mM of DBT, and was determined by gas chromatography (GC) and GC-mass spectrometry. In order to study the desulphurization process by S. marcescens was observed the presence of a sulfur-free product at 16 hours of cultivation. Conclusions The data suggests the use of metabolic pathway “4S” by S. marcescens (UCP 1549) and formed biphenyl. The microbial desulphurization process by Serratia can be suggest significant reducing sulphur content in DBT, and showed promising potential for reduction of the sulfur content in diesel oil.
- Published
- 2012
- Full Text
- View/download PDF
259. Concordância entre diferentes definições operacionais de alteração vocal
- Author
-
Jesus, Luiza Braga de, Carvalho, Fernando Martins, da Silva, Carlos Alberto Lima, and Masson, Maria Lúcia Vaz
- Subjects
Avaliação Acústica ,Métodos Diagnósticos ,Avaliação Perceptivo-auditiva ,Alteração Vocal ,Professores ,Concordância ,Voz - Abstract
Submitted by Pós graduação Saúde, Ambiente e Trabalho (ppgsat4@gmail.com) on 2020-01-13T13:02:40Z No. of bitstreams: 1 Dissertacao LUIZABJ 24-04-2013 consideracoes ML.pdf: 614474 bytes, checksum: 65c18d2839e17b29b70e604acfc37db5 (MD5) Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2020-04-01T21:51:41Z (GMT) No. of bitstreams: 1 Dissertacao LUIZABJ 24-04-2013 consideracoes ML.pdf: 614474 bytes, checksum: 65c18d2839e17b29b70e604acfc37db5 (MD5) Made available in DSpace on 2020-04-01T21:51:41Z (GMT). No. of bitstreams: 1 Dissertacao LUIZABJ 24-04-2013 consideracoes ML.pdf: 614474 bytes, checksum: 65c18d2839e17b29b70e604acfc37db5 (MD5) A prevalência de alteração vocal em professores é muito variável nos estudos, a depender do método diagnóstico utilizado. OBJETIVOS: Avaliar a concordância entre três métodos diagnósticos de alteração vocal (autorreferência, avaliação perceptivo-auditiva e análise acústica (jitter, shimmer, variação de frequência e variação de amplitude) em professores. METODOLOGIA: Estudo transversal, observacional, realizado a partir de dados secundários com 430 docentes do ensino fundamental e médio de 24 escolas municipais da cidade de Salvador- Bahia. A variável “alteração autorreferida” foi obtida por meio de questionário padronizado individual, aplicado no ambiente escolar; a alteração vocal à avaliação perceptivo-auditiva foi obtida meio da escala GRBAS, realizada por fonoaudióloga; a alteração vocal à análise acústica foi avaliada por quatro parâmetros separadamente (variação da frequência, variação da amplitude, jitter e shimmer), obtidos com o programa Multi-dimensional Voice Program (MDVP) modelo 5105 da Kay Elemetrics. A estatística Kappa foi utilizada para medir a concordância entre variáveis. RESULTADOS: A prevalência de alteração vocal variou de acordo com cada definição operacional: autorreferida (62,8%), avaliação perceptivoauditiva (55,1%) e análise acústica (89,3%). A alteração do jitter foi encontrada em 51,2% da amostra, do shimmer em 21,9%, da variação da frequência fundamental em 67,9% e da variação da amplitude em 71,6%. A maior concordância foi observada entre a autorreferência e avaliação perceptivoauditiva (K=0,413; p=0,000) e a menor foi entre autorreferência e shimmer (K=0,081; p=0,016). A análise acústica e seus parâmetros separadamente concordaram mais com a avaliação perceptivo-auditiva do que com a autorreferência. Não houve concordância estatisticamente significante entre análise acústica e autorreferência (K=0,045; p=0,210) e entre variação da amplitude e autorreferência (K=0,090; p=0,057). CONCLUSÕES: A autorreferência, o jitter e a variação de frequência podem ser considerados bons preditores de alteração vocal em professores, já que apresentaram maiores valores de concordância com a avaliação perceptivo-auditiva, considerada padrão-ouro. Os diferentes métodos diagnósticos de alteração vocal são complementares na clínica fonoaudiológica, porém, para estudos populacionais sugere-se que a autorreferência de alteração vocal seja preferida aos demais métodos utilizados neste estudo.
- Published
- 2013
260. Qualidade e Comunicação nas Organizações de Saúde: Aplicação Prática no HCIS
- Author
-
Quintino, Paula, Margarida Saraiva, Da Silva, Carlos Alberto, Saraiva, Margarida, and Teixeira, António
- Subjects
Inovação ,Organizações de Saúde ,Qualidade ,Comunicação - Abstract
Discute-se a cada vez maior exigência de qualidade nas organizações de saúde, que deve ser encarada como uma ferramenta de gestão e de mudança, e a necessidade intrínseca de uma melhor e mais abrangente rede de comunicação, que se impõe para a transmissão de informação e conhecimento, numa sociedade que a uma velocidade atroz se encontra em constante mutação e desenvolvimento. Neste sentido, esta temática assume particular importância no contexto actual, pois a qualidade representa o garante da subsistência da organização abrindo caminho à eficiência e à eficácia organizacional. Este trabalho parte do conhecimento da percepção dos enfermeiros, que na organização de saúde estão directa e proporcionalmente relacionados com a satisfação do cliente, na medida em que asseguram a manutenção dos padrões de qualidade, que a organização pretende alcançar e comunicar, sendo fundamentais na imagem global transmitida e, consequentemente, na criação de valor da própria organização.
- Published
- 2010
261. Racial Disparities in Medication Use During Pregnancy: Results from the NISAMI Cohort.
- Author
-
de Castro CT, Leal LF, Ramos DO, Santana JDM, Cordeiro RC, Rivemales MDCC, de Araújo EM, da Silva CAL, Pereira M, and Dos Santos DB
- Abstract
Purpose: This study aimed to evaluate racial disparities in medication use and associated factors among pregnant women receiving prenatal care at Brazilian Unified Health System primary care health units in the northeast region., Patients and Methods: A total of 1058 pregnant women in the NISAMI Cohort were interviewed between June 2012 and February 2014. Medicines used during pregnancy were classified according to the Anatomical Therapeutic Chemical (ATC) classification system and ANVISA pregnancy risk categories. Prevalence ratios (crude and adjusted) and 95% confidence intervals (CIs) were estimated using Poisson regression with robust error variance. All analyses were stratified by race (Asian, black, brown/mixed, Brazilian indigenous, and white)., Results: Approximately 84% of the pregnant women used at least one medication, with a lower proportion among white women. The most reported medications were antianemic preparations (71.08%; 95% CI 68.27-73.72%), analgesics (21.74%; 95% CI 19.36-24.32%), and drugs for functional gastrointestinal disorders (18.81%; 95% CI 16.57-21.28%). Approximately 29% of women took potentially risky medications during pregnancy, with a higher prevalence among Asian and white women. Factors associated with medication use during pregnancy include a greater number of prenatal consultations, higher education levels, health problems, and smoking. In addition, maternal age above 25 years, smoking status, and two or more previous pregnancies were associated with potentially risky medication use during pregnancy., Conclusion: A high prevalence of medication use during pregnancy was found; however, this prevalence was lower among white women. Nonetheless, black and brown women used antianemic preparations less frequently. This finding suggests that race is a factor of inequity in prenatal care, demanding public policies to mitigate it., Competing Interests: The authors declare no conflicts of interest in this work., (© 2024 Castro et al.)
- Published
- 2024
- Full Text
- View/download PDF
262. Anabolic androgenic steroid-induced hypogonadism, a reversible condition in male individuals? A systematic review.
- Author
-
Vilar Neto JO, da Silva CA, Bruno da Silva CA, Pinto DV, Caminha JSR, de Matos RS, Nunes Filho JCC, Alves FR, Magalhães SC, and De Francesco Daher E
- Subjects
- Androgens adverse effects, Humans, Male, Steroids, Testosterone Congeners adverse effects, Anabolic Agents adverse effects, Hypogonadism chemically induced, Substance-Related Disorders complications
- Abstract
The anabolic-androgenic steroids (AAS) are clinically used as an androgen replacement, in hypogonadism treatment, to induce puberty, and also in the treatment of chronic degenerative diseases. The AAS use out of clinical context is becoming massively, being used merely for aesthetic reasons. AAS abuse may cause severe disarrangement on the HPG axis and generate a significant decrease in testosterone synthesis and secretion by the testes. This review aims to evaluate whether the hypogonadism induced by AAS abuse is reversible and under what circumstances the reversibility is possible. For this, PRISMA guidelines and several databases are used between July and September 2020. Altogether, this systematic review identified and analysed 179 cases of AAS users. Of these, 168 cases had the hypogonadism clearly diagnosed and proven to be linked exclusively to AAS abuse. However, between these 168 cases, only 38 cases presented fully known outcomes and among these, merely in 4, the hypogonadism was completely reversible (2 based on drug therapy) with HPG axis recovery. In conclusion, this review presents evidences that AAS-induced hypogonadism is a seriously underestimated problem, and in the majority of cases, full recovery is very difficult to succeed., (© 2021 Wiley-VCH GmbH.)
- Published
- 2021
- Full Text
- View/download PDF
263. Deoxyribonucleic Acid Extraction and Quantification from Human Saliva Deposited on Foods with Bitemarks.
- Author
-
Musse JO, Marques JAM, Remualdo V, Pitlovanciv AK, da Silva CAL, Corte-Real F, Vieira DN, Vieira WA, Paranhos LR, and Corte-Real AT
- Subjects
- Humans, DNA, Food, Forensic Dentistry, Saliva
- Abstract
Aim: The aim of this study is to evaluate the potential of deoxyribonucleic acid (DNA) recovery from bite marks in foods, in different collection types, from DNA quantification., Materials and Methods: The sample consisted of 80 swabs, obtained from 20 cheese pieces, bitten by the same person, using the double-swab technique in the center and the periphery of the bite. A statistical analysis was carried out using Statistical Package for the Social Sciences (SPSS) statistical software version 20.0, with values of p < 0.05 being considered statistically significant., Results: The DNA was recovered in all cheese pieces, regardless of the collection types and the bite region. However, the comparative analysis of DNA recovery potential in the four swabs allowed us to infer that the collections in the central region of the bite (DC and WC) were the ones that presented better precision, in addition to extracting a higher DNA concentration, the dry swab being in the center of the bite which presented better results., Conclusion: The results proved the effectiveness of the double-swab technique for collecting genetic materials in bite marks; however, in the food used, a single collection at the center of the bite would be enough, optimizing the resources and time needed for the analysis., Clinical Significance: Due to the difficulties of physically comparing a site of a skin lesion and the dental arches of the suspect, the evidence of DNA in saliva has been used to indicate the perpetrator of the bite. In addition, the collection, preservation, and isolation of saliva DNA can be done at low cost and provide flexibility for clinical and laboratory workflow.
- Published
- 2019
264. [Clinical-epidemiological profile of orofacial fissures in a reference center from northeast Brazil].
- Author
-
Rios Moura J, Eufrázio do Nascimento Andrade AP, Lima da Silva CA, De Andrade Santos PP, Souza Freitas V, and Costa das Mercês E
- Abstract
Objective: To describe the clinical-epidemiological profile of patients with orofacial fissures treated at a reference center from northeast Brazil., Materials and Methods: Descriptive study, based on secondary data obtained from medical records. Sociodemographic information of patients and their mothers, use of medication during pregnancy, clinical and surgical aspects related to fissures, family history of malformation, and consanguinity among the parents were investigated. Data were analyzed descriptively using the SPSS (Statistical Package for Social Sciences) statistical program to obtain frequency, mean and standard deviation measures., Results: 51.1% of the patients with orofacial fissures were female, 46.2% were under one year of age and 54.4% lived in urban areas. The majority of mothers were between the ages of 16 and 25 during the gestational period, and 59.2% reported the use of medication. Cleft lip and palate were the most prevalent types of fissures (34.4%). On admission to the Craniofacial Anomaly Rehabilitation Center, 90.5% of the individuals had not yet undergone surgical treatment. Family history of fissure was found in 29.8% of the cases studied, but inbreeding among parents was reported in only 7.1% of them., Conclusion: Socioeconomic and genetic factors can influence the development of orofacial fissures; this requires greater governmental attention as well as new studies for better investigation.
- Published
- 2019
- Full Text
- View/download PDF
265. Multiscale entropy analysis of heart rate variability in heart failure, hypertensive, and sinoaortic-denervated rats: classical and refined approaches.
- Author
-
Silva LE, Lataro RM, Castania JA, da Silva CA, Valencia JF, Murta LO Jr, Salgado HC, Fazan R Jr, and Porta A
- Subjects
- Animals, Autonomic Nervous System physiology, Denervation, Entropy, Male, Rats, Rats, Inbred SHR, Rats, Inbred WKY, Rats, Wistar, Respiratory Mechanics, Vagus Nerve, Heart Failure physiopathology, Heart Rate, Hypertension physiopathology, Sinus of Valsalva
- Abstract
The analysis of heart rate variability (HRV) by nonlinear methods has been gaining increasing interest due to their ability to quantify the complexity of cardiovascular regulation. In this study, multiscale entropy (MSE) and refined MSE (RMSE) were applied to track the complexity of HRV as a function of time scale in three pathological conscious animal models: rats with heart failure (HF), spontaneously hypertensive rats (SHR), and rats with sinoaortic denervation (SAD). Results showed that HF did not change HRV complexity, although there was a tendency to decrease the entropy in HF animals. On the other hand, SHR group was characterized by reduced complexity at long time scales, whereas SAD animals exhibited a smaller short- and long-term irregularity. We propose that short time scales (1 to 4), accounting for fast oscillations, are more related to vagal and respiratory control, whereas long time scales (5 to 20), accounting for slow oscillations, are more related to sympathetic control. The increased sympathetic modulation is probably the main reason for the lower entropy observed at high scales for both SHR and SAD groups, acting as a negative factor for the cardiovascular complexity. This study highlights the contribution of the multiscale complexity analysis of HRV for understanding the physiological mechanisms involved in cardiovascular regulation., (Copyright © 2016 the American Physiological Society.)
- Published
- 2016
- Full Text
- View/download PDF
266. [Dual contraception adherence among HIV-infected women].
- Author
-
Brandão Kde S, Lima BG, Travassos AG, de Brito Fde O, de Souza EX, Haguihara T, and da Silva CA
- Subjects
- Adolescent, Adult, Cross-Sectional Studies, Female, Humans, Middle Aged, Young Adult, Condoms, Contraceptive Agents, Female, HIV Infections prevention & control, HIV Infections transmission, Medroxyprogesterone Acetate, Patient Compliance statistics & numerical data
- Abstract
Purpose: To determine adherence to dual contraception using depot-medroxyprogesterone acetate (DMPA) and condom among HIV-infected women., Methods: A cross-sectional study carried out from December 2013 to September 2014 at a local reference center, with application of questionnaire elaborated after Delphi panel and content validation to 114 HIV(+) women aged 15 to 49 years, using DMPA plus condom for contraception., Results: Mean age was 33.2 ± 7.2 years, mean time since HIV detection was 8.1 ± 5.2 years, mean time of antiretroviral use was 6.8 ± 5 years and mean CD4 cells/mm3 count was 737.6 ± 341.1. Sexual HIV acquisition was reported by 98.2% (112/114), antiretroviral use by 85.9% (98/114), and 77.7% (84/114) had a CD4>500/mm3 count. Having a single sex partner was reported by 78.9% (90/114), with HIV serodiscordance in 41.2% (47/114) of couples, 21.9% did not know the serological status of their partner and in 37.7% of cases (43/114) the partner was unaware of the HIV(+) status of the woman. The last pregnancy was unplanned in 71.9% of cases (82/114) and 14.9% of the women had become pregnant the year before, with pregnancy being unplanned in 70.5% (12/17) of cases. Current use of DMPA was reported by 64.9% (74/114), with genital bleeding in 48.2% (55/114) and weight gain in 67.5% (77/114). Use of a male condom was reported by 62.2% of the subjects (71/114). Three reported that they always used a female condom and ten that they eventually used it. Unprotected vaginal sex was reported by 37.7% (43/114) and unprotected anal intercourse was reported by 32.4% (37/114). Partner resistance to use a condom occurred in 30.7% of cases (35/114). Dual contraception using DMPA with condom was reported by 42.9% (49/114). A partner who resisted wearing a condom was associated with poor adhesion (PR=0.3; 95%CI 0.2-0.7; p<0.001). A partner who was unaware that a woman was infected with HIV favored adherence (PR=1.8; 95%CI 1.2-2.7; p=0.013)., Conclusion: The percentage of dual contraception using DMPA plus condom was 42.9%, maintaining unplanned pregnancies and unprotected sex. Resistance of partners to use a condom increased three times the chance of a woman not adhering to dual contraception, and the partner not knowing women's HIV infection almost doubled the chance to adhere to safe contraception., Goals: to offer new hormonal contraceptives and to involve the partners in contraception and serologic detection tests.
- Published
- 2015
- Full Text
- View/download PDF
267. Toll-like receptor 9 plays a key role in the autonomic cardiac and baroreflex control of arterial pressure.
- Author
-
Rodrigues FL, Silva LE, Hott SC, Bomfim GF, da Silva CA, Fazan R Jr, Resstel LB, Tostes RC, and Carneiro FS
- Subjects
- Animals, Atropine Derivatives, Behavior, Animal, Bradycardia chemically induced, Bradycardia genetics, Bradycardia immunology, Bradycardia physiopathology, Cardiovascular System immunology, Conditioning, Psychological, Disease Models, Animal, Fear, Heart Rate, Male, Mice, Inbred C57BL, Mice, Knockout, Propranolol, Signal Transduction, Tachycardia chemically induced, Tachycardia genetics, Tachycardia immunology, Tachycardia physiopathology, Time Factors, Toll-Like Receptor 9 deficiency, Toll-Like Receptor 9 genetics, Vagus Nerve immunology, Vagus Nerve physiopathology, Arterial Pressure, Baroreflex, Bradycardia metabolism, Cardiovascular System innervation, Immunity, Innate, Tachycardia metabolism, Toll-Like Receptor 9 metabolism, Vagus Nerve metabolism
- Abstract
The crosstalk between the immune and the autonomic nervous system may impact the cardiovascular function. Toll-like receptors are components of the innate immune system and play developmental and physiological roles. Toll-like receptor 9 (TLR9) is involved in the pathogenesis of cardiovascular diseases, such as hypertension and heart failure. Since such diseases are commonly accompanied by autonomic imbalance and lower baroreflex sensitivity, we hypothesized that TLR9 modulates cardiac autonomic and baroreflex control of arterial pressure (AP). Toll-like receptor 9 knockout (TLR9 KO) and wild-type (WT) mice were implanted with catheters into carotid artery and jugular vein and allowed to recover for 3 days. After basal recording of AP, mice received methyl-atropine or propranolol. AP and pulse interval (PI) variability were evaluated in the time and frequency domain (spectral analysis), as well as by multiscale entropy. Spontaneous baroreflex was studied by sequence technique. Behavioral and cardiovascular responses to fear-conditioning stress were also evaluated. AP was similar between groups, but TLR9 KO mice exhibited lower basal heart rate (HR). AP variability was not different, but PI variability was increased in TLR9 KO mice. The total entropy was higher in TLR9 KO mice. Moreover, baroreflex function was found higher in TLR9 KO mice. Atropine-induced tachycardia was increased in TLR9 KO mice, whereas the propranolol-induced bradycardia was similar to WT mice. TLR9 KO mice exhibit increased behavioral and decreased tachycardia responses to fear-conditioning stress. In conclusion, our findings suggest that TLR9 may negatively modulate cardiac vagal tone and baroreflex in mice., (Copyright © 2015 the American Physiological Society.)
- Published
- 2015
- Full Text
- View/download PDF
268. Cholinergic stimulation with pyridostigmine protects myocardial infarcted rats against ischemic-induced arrhythmias and preserves connexin43 protein.
- Author
-
Santos-Almeida FM, Girão H, da Silva CA, Salgado HC, and Fazan R Jr
- Subjects
- Animals, Arrhythmias, Cardiac prevention & control, Atropine Derivatives pharmacology, Blood Pressure, Cholinesterase Inhibitors pharmacology, Male, Myocardial Infarction metabolism, Myocardial Infarction physiopathology, Propranolol pharmacology, Pyridostigmine Bromide pharmacology, Rats, Rats, Wistar, Vagus Nerve drug effects, Vagus Nerve physiology, Arrhythmias, Cardiac drug therapy, Cholinesterase Inhibitors therapeutic use, Connexin 43 metabolism, Heart Rate drug effects, Myocardial Infarction drug therapy, Pyridostigmine Bromide therapeutic use
- Abstract
We investigated the effects of acute pyridostigmine (PYR) treatment, an acetylcholinesterase inhibitor, on arterial pressure (AP), heart rate (HR), cardiac sympathovagal balance, and the incidence of arrhythmias during the first 4 h after myocardial infarction (MI) in anesthetized rats. Male Wistar rats were implanted with catheters into the femoral artery and vein for AP recordings and drug administration. Rats received the autonomic receptor blockers methyl-atropine (1 mg/kg iv) and propranolol (2 mg/kg iv) at intervals of 15 min, 1 h after saline (n=16) or PYR (0.25 mg/kg iv, n=18), to indirectly assess sympathovagal balance. Acute treatment with PYR increased cardiac vagal (86±7 vs. 44±5 beats/min) and decreased sympathetic tone (-31±8 vs. -69±7 beats/min). Different animals were implanted with ECG electrodes and catheters. A large MI was induced via left coronary artery ligation after basal recordings. Rats received PYR (n=14) or saline (n=14) 10-15 min after MI, and the recordings lasted up to 4 h. In part of the animals, hearts were removed for connexin43 quantification after all procedures. MI elicited a fall in AP (-45±5 mmHg), a progressive rise in HR (26±14 beats/min), and an increase in corrected QT interval (33±13 ms). PYR elicited a prompt bradycardia (-50±14 beats/min) that returned to basal levels over time, and it prevented the lengthening of the corrected QT interval. Treatment with PYR increased by ∼20% the occurrence of rats free of arrhythmias after MI. MI markedly decreased connexin43 in left ventricles, and PYR treatment partially prevented this decrease., (Copyright © 2015 the American Physiological Society.)
- Published
- 2015
- Full Text
- View/download PDF
269. The Effect of Protein Restriction in the In Vitro Metabolism of Albendazole in Rats.
- Author
-
Belaz KR, de O Cardoso J, da Silva CA, and Oliveira RV
- Subjects
- Albendazole administration & dosage, Albendazole analogs & derivatives, Animal Nutritional Physiological Phenomena, Animals, Biotransformation, Disease Models, Animal, Male, Microsomes, Liver metabolism, Nutritional Status, Oxidation-Reduction, Protein-Energy Malnutrition etiology, Protein-Energy Malnutrition physiopathology, Rats, Wistar, Albendazole pharmacokinetics, Diet, Protein-Restricted, Liver metabolism, Protein-Energy Malnutrition metabolism
- Abstract
This work presents an in vitro investigation of the effect of protein restriction on the metabolism of albendazole (ABZ). This study was conducted using liver microsomal fractions obtained from Wistar rats. For the quantitative analysis, a multidimensional High Performance Liquid Chromatography (2D HPLC) method was fully validated for the determination of the ABZ metabolites: albendazole sulfoxide, albendazole sulfone and albendazole 2-aminesulfone. The target compounds were directly extracted using a C8-RAM-BSA column (5.0x0.46 cm i.d.) and analyzed on a chromatographic chiral column containing amylose tris(3,5-dimethylphenylcarbamate) (150x4.6 mm i.d.). The in vitro biotransformation results showed that the protein restriction influenced the oxidative metabolism of ABZ. The production of R-(+)-ABZ-SO (1309 nmol/L) and S-(-)-ABZ-SO (1456 nmol/L) was higher in the control animals than in the animals fed with a diet containing 6% protein, which produced 778.7 nmol/L and 709.5 nmol/L for R-(+) and S-(-)-ABZ-SO enantiomers, respectively. These results were statistically inspected by Student´s t test and the results showed a significant difference between the two means (p<0.05). Moreover, the production of ABZ-SO enantiomers was enantioselective where the S-(-)-ABZ-SO was formed in greater amounts than the R-(+)-ABZ-SO in control animals (p=0.0231). However, the enantioselectivity was not observed when the in vitro biotransformation of ABZ was conducted using the microsomal fractions obtained from protein restriction animals (p>0.05). Furthermore, animal nutritional condition could affect the pattern of ABZ sulphoxidation indicating that the protein nutrition affect primarily the formation of R-(+)-ABZSO and S-(-)-ABZ-SO enantiomers.
- Published
- 2015
- Full Text
- View/download PDF
270. High-intensity aerobic training improves endothelium-dependent vasodilation in patients with metabolic syndrome and type 2 diabetes mellitus.
- Author
-
da Silva CA, Ribeiro JP, Canto JC, da Silva RE, Silva Junior GB, Botura E, and Malschitzky MA
- Subjects
- Adult, Aged, Brachial Artery diagnostic imaging, Brazil epidemiology, Diabetes Mellitus, Type 2 drug therapy, Diabetes Mellitus, Type 2 epidemiology, Electrocardiography, Endothelium, Vascular diagnostic imaging, Exercise Test, Female, Humans, Male, Metabolic Syndrome drug therapy, Metabolic Syndrome epidemiology, Middle Aged, Ultrasonography, Brachial Artery physiopathology, Diabetes Mellitus, Type 2 physiopathology, Endothelium, Vascular physiopathology, Endothelium-Dependent Relaxing Factors pharmacology, Exercise, Metabolic Syndrome physiopathology, Vasodilation
- Abstract
Background: The aim of this study is to compare the effect of physical exercise program on the endothelial function of patients with metabolic syndrome and type 2 diabetes mellitus., Methods: Patients were randomized for high intensity aerobic training (HI: 80% maximum heart rate, n=10), low intensity aerobic training (LI: 55% of maximum heart rate, n=10) and control (n=11). Before and after 6 weeks of training, subjects performed the maximal exercise test and a study of the endothelial function, through a high resolution ultrasound of the brachial artery, which was assessed after reactive hyperemia (endothelium dependent vasodilation) and nitrate administration (endothelium independent vasodilation)., Results: A total of 31 patients with metabolic syndrome and type 2 diabetes mellitus were studied, with mean age of 58±6 years, The percentage diameter difference of the vessel after hyperemia was significantly higher for the high intensity group (HI before 2.52±2.85% and after 31.81±12.21%; LI before 3.23±3.52% and after 20.61±7.76%; controls before 3.56±2.33% and after 2.43±2.14%; p<0.05)., Conclusions: High intensity aerobic training improved the functional capability and endothelium dependent vasodilator response, but it does not improve the endothelium independent vasodilation in patients with metabolic syndrome and type 2 diabetes mellitus., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
271. Role of the morphology and polyphosphate in Trichoderma harzianum related to cadmium removal.
- Author
-
Lima Ade F, de Moura GF, de Lima MA, de Souza PM, da Silva CA, Takaki GM, and do Nascimento AE
- Subjects
- Biomass, Microscopy, Electron, Transmission, Pest Control, Biological, Trichoderma ultrastructure, Cadmium isolation & purification, Polyphosphates metabolism, Trichoderma metabolism
- Abstract
This study concerns the metabolism of polyphosphate in Trichoderma harzianum, a biocontrol agent with innate resistance against most chemicals used in agriculture, including metals, when grown in the presence of different concentrations of cadmium. The biomass production was affected by the concentration of metal used. Control cultures were able to accumulate polyphosphate under the conditions used. Moreover, the presence of cadmium induced a reduction in polyphosphate content related to the concentration used. The morphological/ultrastructural aspects were characterized by using optical and scanning electron microscopy, and were affected by the heavy metal presence and concentration. The efficiency of cadmium removal revealed the potential of the microorganism for use in remediation. The data indicate the potential for polyphosphate accumulation by the fungus, as well as its degradation related to tolerance/survival in the presence of cadmium ions.
- Published
- 2011
- Full Text
- View/download PDF
272. Resistance training associated with the administration of anabolic-androgenic steroids improves insulin sensitivity in ovariectomized rats.
- Author
-
Urtado CB, Pereira GB, Urtado MB, de Carvalho EB, Leite Gdos S, Donatto FF, de Oliveira Assumpção C, Leite RD, da Silva CA, de Sales MM, Tibana RA, Alves SC, and Prestes J
- Abstract
The aim of the present study was to investigate the effects of anabolic-androgenic steroids and resistance training (RT) on insulin sensitivity in ovariectomized rats. Adult female Wistar rats were divided into ten experimental groups (n = 5 animals per group): (1) sedentary (Sed-Intact); (2) sedentary ovariectomized (Sed-Ovx); (3) sedentary nandrolone (Sed-Intact-ND); (4) sedentary ovariectomized plus nandrolone (Sed-Ovx-ND); (5) trained (TR-Intact); (6) trained nandrolone (TR-Intact-ND); (7) trained ovariectomized (TR-Ovx); (8) trained ovariectomized plus nandrolone; (9) trained sham; and (10) trained ovariectomized plus sham. Four sessions of RT were used, during which the animals climbed a 1.1 m vertical ladder with weights attached to their tails. The sessions were performed once every 3 days, with between four and nine climbs and with eight to twelve dynamic movements per climb. To test the sensitivity of insulin in the pancreas, glucose and insulin tolerance tests were performed. For insulin sensitivity, there was a statistically significant interaction for the TR-Ovx group, which presented higher sensitivity than the Sed-Intact, Sed-Ovx, and TR-Intact groups. Sed-Intact-ND and TR-Intact-ND groups exhibited higher values of insulin sensitivity than the Sed-Intact group. Except for the TR-Intact group, sensitivity was greater in trained groups than in the Sed-Intact group. There was higher insulin sensitivity in the TR-Intact-ND group than in the Sed-Intact and Sed-Intact-ND groups (P < 0.05). In conclusion, ovariectomy and short-term RT alone induced no change on insulin action. Administration of nandrolone decanoate improved insulin action, mainly when it was associated with RT.
- Published
- 2011
- Full Text
- View/download PDF
273. Sympathetic activity is not increased in L-NAME hypertensive rats.
- Author
-
Dos Santos FM, Martins Dias DP, da Silva CA, Fazan R Jr, and Salgado HC
- Subjects
- Animals, Baroreflex physiology, Blood Pressure physiology, Consciousness physiology, Disease Models, Animal, Enzyme Inhibitors adverse effects, Heart Rate physiology, Hypertension metabolism, Kidney metabolism, Male, Norepinephrine metabolism, Rats, Rats, Wistar, Time Factors, Hypertension physiopathology, Kidney innervation, NG-Nitroarginine Methyl Ester pharmacology, Sympathetic Nervous System physiopathology
- Abstract
The role played by the sympathetic drive in the development of N(G)-nitro-l-arginine methyl ester (l-NAME)-induced hypertension is not firmly established. Therefore, the present study was undertaken in conscious rats in which hypertension was induced by treatment with l-NAME over the course of either 2 or 14 days. Mean arterial pressure (MAP) was measured via a catheter placed in the femoral artery, drugs were administered via a cannula placed in the femoral vein, and renal sympathetic nerve activity (RSNA) was monitored using an implanted electrode. Despite the remarkable increase in arterial pressure, heart rate did not change after treatment with l-NAME. RSNA was similar in l-NAME-induced hypertensive rats treated over the course of 2 or 14 days, as well as in normotensive rats. It was also demonstrated that l-NAME-induced hypertensive rats displayed a resetting of the baroreflex control of RSNA to hypertensive levels, with decreased sensitivity over the course of 2 or 14 days. Furthermore, the sympathetic-vagal balance examined in the time and frequency domain and the renal and plasma norepinephrine content did not differ between groups. In conclusion, the evaluation of the sympathetic drive in conscious rats demonstrated that the arterial hypertension induced by l-NAME treatment over the course of 2 and 14 days does not show sympathetic overactivity.
- Published
- 2010
- Full Text
- View/download PDF
274. The effect of chemical therapy with bleomycin sulfate on the functional parameters of the endocrine pancreas.
- Author
-
da Silva CA, Cancelliero KM, and Costa D
- Subjects
- Animals, Cyclic AMP-Dependent Protein Kinases metabolism, Disease Models, Animal, Glucose Tolerance Test, Glucose Transporter Type 2 metabolism, Homeostasis drug effects, Insulin blood, Islets of Langerhans metabolism, Male, Pancreatic Diseases metabolism, Protein Kinase C metabolism, Rats, Rats, Wistar, Antibiotics, Antineoplastic toxicity, Bleomycin toxicity, Islets of Langerhans drug effects, Pancreatic Diseases chemically induced
- Abstract
Objective: The objective of the present study was to evaluate the effect of bleomycin sulfate on parameters related to the functionality of pancreatic tissue, with emphasis on the glucose tolerance test, insulin tolerance test, insulinemia and static secretion of insulin as well as the insulin receptor, and PKA, PKC and GLUT2 concentrations in the pancreatic islets., Design: Twenty-four male rats were divided into 2 groups: control and treated with bleomycin (2.5 mg/kg, intratracheal mode). After 7 days, the animals were euthanized and the analyses were carried out., Statistics: The normality and the homoscedasticity of the data distribution were tested and ANOVA was applied. The Tukey post hoc test followed ANOVA for the comparison of the static insulin secretion test at different glucose concentrations., Results: In the glucose tolerance test, the bleomycin group showed a larger area (17,306+/-539 mg/dL x 60min) than that of the control group (9,151+/-517 mg/dL x 60 min) and in the insulin tolerance test, there was a greater percentage fall in glycemia (8.08+/-0.56%) in the bleomycin than in the control group (3.87+/-1.14%). The bleomycin group also presented a reduction in insulin secretion and an increase in plasmatic insulin concentration in the static insulin secretion test. With respect to the concentrations of the insulin receptor, GLUT2, PKC and PKA in the pancreatic islets of the bleomycin group, there was an increase in GLUT2 (48.4%) and PKC (70.8%) and a reduction in PKA (38.5%)., Conclusion: During treatment with bleomycin, innumerable chemical-metabolic alterations were unleashed in the tissues which were not primary targets of the chemical therapy and which could compromise the homeostasis of the systems taking part in the glycemic adjustment, predisposing the organism to the development of a pre-diabetic pattern whose degree of incidence or reversibility is still unknown to the scientific community.
- Published
- 2009
275. [Quali-quantitative analysis of influence of sports activities in the health of children and young people in a Program of Sports Education in Natal-Brasil].
- Author
-
Accioly Júnior H, Liparotti JR, Schneider LC, da Silva CA, and Cavalcanti SG
- Subjects
- Adolescent, Body Constitution, Brazil, Chi-Square Distribution, Child, Child Welfare, Humans, Income, Physical Fitness physiology, Program Evaluation, Range of Motion, Articular, Adolescent Development physiology, Child Development physiology, Parents, Physical Education and Training, Poverty, Sports
- Abstract
Purpose: Verify in a specific social totality of lower socioeconomical level, what would be the main interest for parents to place their children in an Educational Program Through Sport. To evaluate and classify children and young people as to physical aptitude related to health through scientifically validated indicators and to relate them to socioeconomical condition. To verify compatibility between qualitative and quantitative researches., Methods: The sample of the qualitative study is composed of 22 subjects, characterizing itself as a non probabilistic sample of the causal type. Semi structured interview techniques in an individual situation and free evocation of words were used, based on the presuppositions of the Theory of Social Representations. A mixed sample, of the quantitative study, is composed of 67 children, where the possibility of relationship of the family income variable was confirmed with the variables: Body Composition and Flexibility, that compose the physical aptitude indicator related to health and the social representation of the parents., Results: The qualitative results refer to biological aspects of health that constitute the central nucleus of the social representation of sport. In the quantitative aspect, it is verified that there was no correlation statistically, significant between the indexes of physical aptitude and the family income., Conclusion: The reason attributed to the physical-sport activities by the parents or relatives of the children, is based on the benefits to biological health and is shown by the children's good performance in the applied aptitude tests. There is not significant correlation between the index of physical aptitude applied to health, which reinforces the possibility of the parents social representation and the children's behavior.
- Published
- 2005
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.