269 results on '"Dell'Antonio G"'
Search Results
252. "Striped" pattern of medullary ray fibrosis in allograft biopsies from kidney transplant recipients maintained on tacrolimus.
- Author
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Dell'Antonio G and Randhawa PS
- Subjects
- Adult, Biopsy, Diabetic Nephropathies surgery, Fibrosis, Humans, Kidney Failure, Chronic etiology, Middle Aged, Transplantation, Homologous, Graft Rejection pathology, Immunosuppressive Agents therapeutic use, Kidney Failure, Chronic surgery, Kidney Medulla pathology, Kidney Transplantation immunology, Kidney Transplantation pathology, Tacrolimus therapeutic use
- Abstract
Background: A striped pattern of fibrosis has been described in the kidneys of patients undergoing long-term cyclosporine or tacrolimus therapy. This lesion is frequently misconstrued as being specific for drug toxicity., Methods: We performed clinicopathologic correlation on 18 patients with striped fibrosis identified by reviewing 61 biopsies from kidney transplant recipients maintained with tacrolimus., Results: Acute rejection was identified in 14 of 18 patients, chronic rejection in 9 of 18 patients, potential diabetic microvascular injury in 8 of 18, and pre-existing donor disease in 2 of 18. In only one patient could striped fibrosis be ascribed primarily to tacrolimus. Striped fibrosis could also be demonstrated in 6 of 10 late allograft biopsy specimens from patients maintained with only azathioprine, and 8 of 10 native biopsies from patients with advanced diabetes mellitus., Conclusion: Multiple insults contribute to the pathogenesis of striped fibrosis in the kidney. This lesion can be attributed entirely to chronic drug toxicity in only a minority of allografts.
- Published
- 1999
- Full Text
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253. Effects of trimetazidine on metabolic and functional recovery of postischemic rat hearts.
- Author
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Allibardi S, Chierchia SL, Margonato V, Merati G, Neri G, Dell'Antonio G, and Samaja M
- Subjects
- Animals, Blood Pressure drug effects, Energy Metabolism drug effects, Heart Rate drug effects, Male, Myocardial Contraction drug effects, Myocardial Reperfusion Injury metabolism, Myocardial Reperfusion Injury pathology, Myocardium metabolism, Myocardium pathology, Rats, Rats, Sprague-Dawley, Myocardial Reperfusion Injury physiopathology, Trimetazidine pharmacology, Vasodilator Agents pharmacology
- Abstract
The objective of this study was to test the hypothesis that the beneficial effect of trimetazidine during reflow of ischemic hearts is mediated by energy sparing and ATP pool preservation during ischemia. Isolated rat hearts (controls and rats treated with 10(-6) M trimetazidine, n = 17 per group) underwent the following protocol: baseline perfusion at normal coronary flow (20 minutes), low-flow ischemia at 10% flow (60 minutes), and reflow (20 minutes). We measured contractile function, O2 uptake, lactate release, venous pH and PCO2, and the tissue content of high-energy phosphates and their metabolites. During baseline, trimetazidine induced higher venous pH and lower PCO2 without influencing performance and metabolism. During low-flow ischemia, trimetazidine reduced myocardial performance (P = 0.04) and ATP turnover (P = 0.02). During reflow, trimetazidine improved performance (91 +/- 6% versus. 55 +/- 6% of baseline), prevented the development of diastolic contracture and coronary resistance, and reduced myocardial depletion of adenine nucleotides and purines. ATP turnover during low-flow ischemia was inversely related to recovery of the rate-pressure product (P = 0.002), end-diastolic pressure (P = 0.007), and perfusion pressure (P = 0.05). We conclude that trimetazidine-induced protection of ischemic-reperfused hearts is also mediated by energy sparing during ischemia, which presumably preserves the ATP pool during reflow.
- Published
- 1998
- Full Text
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254. Amyloid neuropathy simulating lower motor neuron disease.
- Author
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Quattrini A, Nemni R, Sferrazza B, Ricevuti G, Dell'Antonio G, Lazzerini A, and Iannaccone S
- Subjects
- Amyloid Neuropathies physiopathology, Biopsy, Diagnosis, Differential, Humans, Male, Middle Aged, Motor Neuron Disease physiopathology, Neural Conduction physiology, Amyloid Neuropathies diagnosis, Motor Neuron Disease diagnosis
- Abstract
We report a 57-year-old man with progressive symmetric weakness and fasciculation affecting the legs. Electromyography revealed fibrillations and neurogenic motor unit potentials in the leg muscles. Biopsy of a motor branch of the obturator nerve revealed axonal degeneration, loss of myelinated nerve fibers, and amyloidosis with deposits of lambda light chains. At 6-month follow-up, the patient manifested sensory and autonomic symptoms, and lambda light chains were first detected in the serum. In this case, diagnosis of amyloidosis remained elusive until motor nerve biopsy.
- Published
- 1998
- Full Text
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255. Heterogeneity and susceptibility to apoptosis of human renal carcinoma cells in vitro.
- Author
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Dell'Antonio G, Rovere P, Mangili F, and Manfredi AA
- Subjects
- Blotting, Western, Bone Neoplasms metabolism, Carcinoma, Renal Cell metabolism, Carcinoma, Renal Cell secondary, Carcinoma, Renal Cell ultrastructure, Cell Size drug effects, Fas Ligand Protein, Humans, Immunohistochemistry, Insulin pharmacology, Kidney Neoplasms metabolism, Kidney Neoplasms ultrastructure, Membrane Glycoproteins metabolism, Microscopy, Electron, Phenotype, Tretinoin pharmacology, Tumor Cells, Cultured, Apoptosis, Bone Neoplasms secondary, Carcinoma, Renal Cell pathology, Kidney Neoplasms pathology, Sacrum
- Abstract
Tumors are heterogeneous in terms of morphology and susceptibility to drugs or radiation. Among primary and metastatic cells of a human renal carcinoma, a population (type II) of larger cells with prominent nucleoli, eosinophilic globules of intermediate filaments in paranuclear bundles, margination of subcellular organelles and peripheral pools of glycogen was evident. Paranuclear structures were recognized by monoclonal antibodies specific for cytokeratin 8, 18 and 19, but not by vimentin specific antibodies. We propagated a cell line in vitro (referred to as BKR cells), and observed culture in vitro, the almost complete disappearance of the type II cells. Pharmacological agents that influence cell differentiation, such as retinoic acid, rescued the expression of type II cells in vitro. Long-term treatments with insulin or alpha-interferon, but not with the epithelial growth factor (EGF), similarly differentiated BKR cells and abated their susceptibility to spontaneous and actinomycin-D induced apoptosis. These data support the contention that differentiation of tumor cells is actively maintained in vivo and further strengthen the caveat on tumor lines stabilized in vitro, that poorly represent the morphologic and antigenic heterogeneity of neoplasms in vivo.
- Published
- 1998
256. Nonacidotic proximal tubulopathy transmitted as autosomal dominant trait.
- Author
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Vezzoli G, Zerbi S, Baragetti I, Soldati L, Mora S, Dell'Antonio G, and Bianchi G
- Subjects
- Absorption, Adolescent, Adult, Calcitriol metabolism, Calcium blood, Calcium urine, Child, Chronic Kidney Disease-Mineral and Bone Disorder complications, Diagnosis, Differential, Fanconi Syndrome diagnosis, Female, Humans, Hypophosphatemia complications, Kidney Calculi complications, Kidney Diseases diagnosis, Kidney Diseases metabolism, Kidney Tubules, Proximal metabolism, Male, Middle Aged, Pedigree, Kidney Diseases genetics
- Abstract
The family of a patient with a nonacidotic and hypercalciuric proximal tubulopathy was studied. The proband showed glycosuria, aminoaciduria, tubular proteinuria, renal hypophosphatemia, and urate tubular hyporeabsorption without bicarbonate loss. He also presented increased urine calcium excretion, plasma 1,25-dihydroxyvitamin D, and enteral calcium absorption. Clinical consequences of the tubulopathy were osteopenia and calcium kidney stones. Fifteen of the proband's relatives were studied; six of them had renal hypophosphatemia, 10 presented hypercalciuria, and three showed both hypercalciuria and hypophosphatemia. No other reabsorption defects were observed. High plasma levels of 1,25-dihydroxyvitamin D were found in 13 family members; their values correlated positively with calcium excretion and negatively with tubular phosphate reabsorption. None produced stones or had reduced mineral bone density. Hypophosphatemia and hypercalciuria occurred in the two generations studied; their transmission was independent of gender, and male-to-male transmission occurred for both defects. Our findings suggest that a genetic alteration of proximal tubular function could cause multiple reabsorption defects in the proband or renal phosphate leakage in the proband's relatives. The genotypic alteration causing the proximal dysfunctions may be monogenic, with an autosomal dominant pattern of inheritance and variable expressivity. Increased calcium excretion may be due to the proximal tubular alteration; alternatively, it may be the result of a genetic background predisposing to idiopathic hypercalciuria. Phosphate and calcium loss could stimulate 1,25-dihydroxyvitamin D synthesis in proximal tubular cells.
- Published
- 1997
- Full Text
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257. Cancer arising after pancreas and/or kidney transplantation in a series of 99 diabetic patients.
- Author
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Martinenghi S, Dell'Antonio G, Secchi A, Di Carlo V, and Pozza G
- Subjects
- Adult, Diabetes Mellitus, Type 1 mortality, Follow-Up Studies, Humans, Immunosuppressive Agents therapeutic use, Kidney Transplantation methods, Kidney Transplantation mortality, Middle Aged, Neoplasms classification, Neoplasms mortality, Pancreas Transplantation methods, Pancreas Transplantation mortality, Survival Analysis, Diabetes Mellitus, Type 1 complications, Diabetes Mellitus, Type 1 surgery, Kidney Transplantation adverse effects, Neoplasms etiology, Pancreas Transplantation adverse effects
- Abstract
Objective: Recipients of solid organ transplants have an increased risk of developing certain types of malignancies as compared with the general population. The majority of the literature has reported on neoplasms in kidney and heart transplant recipients., Research Design and Methods: We describe 9 neoplasms occurring in 7 out of 73 IDDM patients after simultaneous pancreas and kidney transplantation. No cases were recorded among 26 IDDM recipients of kidney transplantation., Results: Among the neoplasms found were 2 cases of posttransplant lymphoproliferative disorder (PTLD), malignant melanoma, basal-cell and squamous-cell carcinoma of the skin in the same patient, squamous-cell carcinoma in situ of the vulva, hepatocarcinoma, small-cell lung cancer, and ductal carcinoma of the breast. Four patients died. Among immunological risk factors, over-immunosuppression for steroid-resistant kidney rejection was administered only in the 2 cases of PTLD., Conclusions: Increased dosage of immunosuppressive agents may be necessary in some patients of prevent or treat rejection in view of their reduced survival on hemodialysis.
- Published
- 1997
- Full Text
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258. Nonacidotic kidney proximal tubulopathy with absorptive hypercalciuria.
- Author
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Vezzoli G, Corghi E, Edefonti A, Palazzi P, Dell'Antonio G, Elli A, Azzani T, Vallino F, and Bianchi G
- Subjects
- Amino Acids metabolism, Biopsy, Child, Preschool, Family Health, Glucose metabolism, Humans, Kidney Diseases genetics, Kidney Diseases pathology, Male, Middle Aged, Phosphates metabolism, Proteins metabolism, Uric Acid metabolism, Calcium urine, Kidney Diseases urine, Kidney Tubules, Proximal metabolism, Kidney Tubules, Proximal pathology
- Abstract
We studied three patients with proximal tubulopathy characterized by defective reabsorption of phosphate, glucose, amino acids, urate, and low molecular weight proteins. This tubulopathy differs from Fanconi syndrome in that the patients had normal plasma bicarbonate and absorptive hypercalciuria associated with increased 1,25-dihydroxyvitamin D levels. The youngest patient was rachitic and may be classified with previously described patients, whereas the other two patients presented with nonrachitic osteopenic bone disease and their tubulopathy started during adult life. Kidney defects appeared sequentially in one of the nonrachitic patients. The two brothers of the youngest patient had similar kidney and bone disturbances. One of the other two patients had a brother with similar kidney reabsorption defects; an additional brother was probably affected and a sister presented with glycosuria, but no other reabsorption defects. The findings in these two families suggest a genetic transmission of proximal tubulopathy. The third case was sporadic. Renal histology of the three patients showed a great number of giant cells in the tubular lumen. We conclude that, at least in our adult patients, tubulopathy may represent a new entity among the proximal tubular dysfunction cases described to date. The features of this proximal defect suggest that it may be caused by a selective alteration of luminal cell membrane transport of phosphate, glucose, amino acids, urate, and proteins in the presence of a normal sodium gradient across the tubular cell membrane.
- Published
- 1995
- Full Text
- View/download PDF
259. Percutaneous microbiopsy for the diagnosis of rejection in whole bladder-diverted pancreas transplantation.
- Author
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Martinenghi S, Dell'Antonio G, Secchi A, Bernardi M, Faravelli T, Castoldi R, Angeli E, Staudacher C, Gallioli G, and Di Carlo V
- Subjects
- Biopsy, Needle methods, Blood Grouping and Crossmatching, Contraindications, Humans, Pancreas Transplantation methods, Diabetes Mellitus, Type 1 surgery, Graft Rejection pathology, Pancreas Transplantation pathology, Urinary Bladder surgery
- Published
- 1994
260. Culture of human endometrial cells: a new simple technique to completely separate epithelial glands.
- Author
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Viganò P, Di Blasio AM, Dell'Antonio G, and Vignali M
- Subjects
- Endometrium chemistry, Epithelial Cells, Female, Humans, Immunohistochemistry, Keratins analysis, Stromal Cells cytology, Vimentin analysis, Cell Separation methods, Cells, Cultured cytology, Endometrium cytology
- Abstract
In vitro studies on dispersed human endometrial cells are still difficult to perform as the techniques employed do not allow an optimal separation of the cells. In particular, epithelial glands tend to maintain their tubular structure even after enzymatic dispersion. This could be a disadvantage when monolayers of single well dispersed cells are needed. In this paper we describe a new technique to establish monolayer cultures of isolated endometrial stromal and epithelial cell populations. After a first collagenase digestion, stromal and epithelial cells were separated by differential sedimentation at unity gravity. The epithelial glands obtained were further dispersed in single cells using a short incubation with low amount of trypsin. Morphologic characterization was performed using immunohistochemistry for vimentin and cytokeratins. Compared to previous described methods, this procedure is shorter and could better preserve cell surface structures. Thus, it could be successfully employed for in vitro studies of human endometrial pathophysiology.
- Published
- 1993
- Full Text
- View/download PDF
261. Electron microscopy of fine-needle aspiration biopsy from extragonadal germ cell tumors.
- Author
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Dell'Antonio G, Taccagni GL, Terreni MR, Leone BE, and Cantaboni A
- Subjects
- Adult, Biopsy, Needle, Female, Humans, Male, Microscopy, Electron, Middle Aged, Mediastinal Neoplasms ultrastructure, Neoplasms, Germ Cell and Embryonal ultrastructure, Retroperitoneal Neoplasms ultrastructure
- Abstract
We describe five cases of extragonadal germ cell tumor (EGCT) diagnosed by the electron microscope (EM) on cytological material. The clinical diagnosis was incorrect in all cases and EGCT was suspected in two cases; cytological diagnosis by light microscopy confirmed the presence of malignant tumor cells, but did not identify the cytotype/s correctly except in one case. Ultrasonography, laparoscopy, and autopsy (in case 3) excluded a primitive germ cell tumor (GCT). Histology confirmed the EM diagnosis in all cases. EM, even of scanty or necrotic cytological material, is particularly useful for mediastinal and retroperitoneal masses. In case of EGCT, EM can identify the different cytotypes and the different ultrastructural subcellular cytotypes and demonstrates a close relation between seminomatous and nonseminomatous GCT, which could influence their classification and prognosis.
- Published
- 1992
- Full Text
- View/download PDF
262. Evaluation of histological and ultrastructural aspects of endometrium during treatment with gestrinone in women with amenorrhea or spotting.
- Author
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Fedele L, Marchini M, Baglioni A, Dell'Antonio G, and Motta T
- Subjects
- Adult, Amenorrhea etiology, Female, Gestrinone administration & dosage, Humans, Uterine Hemorrhage chemically induced, Amenorrhea pathology, Endometriosis drug therapy, Endometrium ultrastructure, Gestrinone therapeutic use, Norpregnatrienes therapeutic use, Uterine Hemorrhage pathology, Uterine Neoplasms drug therapy
- Abstract
Nineteen patients with a laparoscopic diagnosis of endometriosis were treated with gestrinone at a dosage of 2.5 mg twice a week for 6 months. In 7 who reported spotting in the first 3 months, the dose was increased to 2.5 mg three times a week during the second trimester. An endometrial sample was obtained from each patient at the time of laparoscopy (basal) and at 3 and 6 months of treatment. Endometrial structure and ultrastructure were studied. Areas of hemorrhage and of loss of surface epithelium and a lesser degree of involution of the surface epithelium were observed in the 3-month samples of 7 patients with spotting, compared with 12 with amenorrhea. It is hypothesized that incomplete endometrial involution could be due to differences in gestrinone pharmacokinetics in individual patients, in the quality and/or quantity of endometrial cytosolic receptors for sex steroids, or in endocrine compensation to administration of the drug.
- Published
- 1990
- Full Text
- View/download PDF
263. Heterogeneous subcellular morphology of lung adenocarcinoma cells: identification of different cytotypes on cytological material.
- Author
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Taccagni G, Dell'Antonio G, Terreni MR, and Cantaboni A
- Subjects
- Adult, Aged, Biopsy, Needle, Cell Nucleus ultrastructure, Cytoplasm ultrastructure, Cytoplasmic Granules ultrastructure, Female, Glycogen analysis, Humans, Lysosomes ultrastructure, Male, Microscopy, Electron, Middle Aged, Neoplasm Staging, Pleural Effusion pathology, Vacuoles ultrastructure, Adenocarcinoma ultrastructure, Lung Neoplasms ultrastructure
- Abstract
We performed an electron microscopic study of cytologic material from 20 cases of primary lung adenocarcinoma (pleural effusion, 13 cases; fine needle aspiration biopsy, 7 cases). Ultrastructural characteristics related to secretory and storing activity of adenocarcinoma cells were evaluated semiquantitatively. Data analysis identified three basic cell types (secretory, well or poorly differentiated; storing; and indifferent). We could classify our cases in five groups of pure or mixed cytotypes. Our results demonstrated a subcellular morphological heterogeneity manifested by the presence of different basic cell types: secretory, storing or indifferent features (or both); and different secretory and storage products in the same cell. This heterogeneity of lung adenocarcinoma cells suggests that the neoplasm could arise from a single cell type capable of differentiating along different lines. Cases lacking secretory differentiation seemed to be characterized by more aggressive biologic behavior. A clear correlation between the ultrastructural cytotypes identified and the clinical and prognostic data on the patients was not observed. This may be due to the fact that 75% of the patients were in clinical stage III at the time of diagnosis; also, in this series only five cases did not have characteristics that indicated secretory activity.
- Published
- 1990
- Full Text
- View/download PDF
264. Chromogranin A as a marker of neuroendocrine histogenesis of tumours: an immunoelectron microscopic study with considerations about the influence of fixation and embedding media on immunolabelling.
- Author
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Leone BE, Taccagni GL, Dell'Antonio G, and Cantaboni A
- Subjects
- Adrenal Gland Neoplasms pathology, Adrenal Gland Neoplasms ultrastructure, Biomarkers, Tumor analysis, Cell Transformation, Neoplastic pathology, Cell Transformation, Neoplastic ultrastructure, Chromogranin A, Colonic Neoplasms pathology, Colonic Neoplasms ultrastructure, Humans, Immunohistochemistry methods, Lung Neoplasms pathology, Lung Neoplasms ultrastructure, Microscopy, Electron methods, Pancreatic Neoplasms pathology, Pancreatic Neoplasms ultrastructure, Parathyroid Neoplasms pathology, Parathyroid Neoplasms ultrastructure, Thyroid Neoplasms pathology, Thyroid Neoplasms ultrastructure, Adrenal Gland Neoplasms metabolism, Cell Transformation, Neoplastic metabolism, Chromogranins metabolism, Colonic Neoplasms metabolism, Lung Neoplasms metabolism, Pancreatic Neoplasms metabolism, Parathyroid Neoplasms metabolism, Thyroid Neoplasms metabolism
- Abstract
The ultrastructural localization of chromogranin A (Chr A) was studied in eleven neoplasias of the diffuse neuroendocrine system (3 pancreatic islet-cell tumours, 1 medullary carcinoma of the thyroid, 1 large bowel and 1 small bowel carcinoid tumours, 2 carcinoid tumours of the lung, 1 adenoma of the parathyroid gland, 2 pheochromocytomas of the adrenal gland). On account of the great influence of the technical treatment of the samples on the immunolocalization of Chr A, the effect of the following variables was studied in a case of pheochromocytoma: fixation in glutaraldehyde versus paraformaldehyde, postfixation in osmium tetroxide versus omission, embedding in epoxy resin versus acrylic resin. The method of choice for the better preservation of the antigenic character of the tissue was found to be fixation in 4% paraformaldehyde, omission of osmium postfixation and embedding in LRWhite acrylic resin; by this procedure we were able to find Chr A in the neurosecretory granules of all the studied cases, using three commercially available antibodies directed against Chr A. These findings further confirm that Chr A is a reliable marker for the study of neuroendocrine neoplasias by electron microscopy.
- Published
- 1990
265. [Tumors of the smooth muscles of the uterus with epithelioid and star-like sclerotic appearance].
- Author
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Baglioni A, Taccagni GL, Dell'Antonio G, Marchini M, Grassi R, Uber E, Fedele L, and Carinelli SG
- Subjects
- Aged, Female, Humans, Leiomyoma ultrastructure, Sclerosis, Uterine Neoplasms ultrastructure, Leiomyoma pathology, Muscle, Smooth pathology, Uterine Neoplasms pathology
- Published
- 1986
266. Pure apocrine carcinoma of the female breast presenting as a cyst: a case report.
- Author
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Taccagni GL, Sironi M, Dell'Antonio G, and Cantaboni A
- Subjects
- Aged, Apocrine Glands pathology, Breast Neoplasms diagnostic imaging, Carcinoma, Intraductal, Noninfiltrating pathology, Female, Humans, Radiography, Breast Neoplasms pathology, Fibrocystic Breast Disease pathology
- Published
- 1985
267. [Primary neuroendocrine carcinoma of the skin: histologic, immunohistochemical and ultrastructural study of a case with highly aggressive biological behavior].
- Author
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Taccagni GL, Saccomanno K, Dell'Antonio G, Sironi M, and Vicari A
- Subjects
- Abscess complications, Apudoma etiology, Buttocks, Carcinoma, Merkel Cell etiology, Female, Hormones, Ectopic analysis, Humans, Injections, Intramuscular adverse effects, Middle Aged, Neoplasm Proteins analysis, Skin Neoplasms etiology, Apudoma pathology, Carcinoma, Merkel Cell pathology, Skin Neoplasms pathology
- Abstract
We report a histological, immunohistochemical and ultrastructural study of a case of neuroendocrine carcinoma of the skin, which occurred in a 52 years old woman in the dermal, subcutis and soft tissues of the left buttock. Clinically this neoplasm, which was related to intramuscular injections and a to a following abscess, had rapidly reached unusual dimension compared with other cases of neuroendocrine carcinoma of the skin reported in the literature. The patient died three months after presentation with distant metastases. A variable percentage of the neoplastic cells was positive for low molecular weight cytokeratins (CK), neurofilaments (NF), neuron specific enolase (NSE) and occasionally for vaso-intestinal polypeptide (VIP). Ultrastructural investigations showed two types of neoplastic cells, identified for some features of the nucleus and of the cytoplasm; these two groups of cells are, in our opinion, related to different stages of cellular development. Our results, in agreement with some observations reported in the literature, give evidence of a possible origin of neuroendocrine carcinoma of the skin from an undifferentiated cell which is capable of neuroendocrine or ectodermal differentiation.
- Published
- 1989
268. [Epithelial sarcoma. Immunohistochemical and ultrastructural study of a case].
- Author
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Taccagni GL, Sironi M, Dell'Antonio G, Leone BE, and Parafioriti A
- Subjects
- Adult, Axilla, Humans, Immunohistochemistry, Intermediate Filament Proteins analysis, Male, Microscopy, Electron, Sarcoma ultrastructure, Skin Neoplasms ultrastructure
- Published
- 1988
269. Mixed germ cell tumour of the mediastinum (seminoma, embryonal carcinoma, choriocarcinoma and teratoma). Light and electron microscopic cytology and histological investigation.
- Author
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Taccagni GL, Parafioriti A, Dell'Antonio G, and Crespi G
- Subjects
- Adult, Humans, Male, Microscopy, Electron, Neoplasms, Germ Cell and Embryonal ultrastructure
- Abstract
A mixed germ cell tumour of the mediastinum was found in a 27-year-old subject. It had four components: seminoma, embryonal carcinoma, choriocarcinoma and teratoma. Material for light microscopy (L.M.) and ultrastructural studies were obtained with computerized tomography (CT) guided fine needle aspiration. Three histotypes were identified with ultrastructural studies while only seminoma was identified with L.M. The slides prepared for L.M. were reexamined in the light of findings with electron microscopy (E.M.) and previously unidentified cellular elements were found to be characteristic of choriocarcinoma and teratoma. Histological examination of surgical and autopsy material confirmed the cytological findings. Because of the singularity of this case, the rarity of this type of neoplasm and the difficulty in arriving at a differential diagnosis from other neoplastic and non neoplastic lesions, only the ultrastructural cytological examination allowed us to identify the mixed nature of the tumour. Such an identification is not always possible with L.M. but it is a determining factor in the prognosis and therapy.
- Published
- 1989
- Full Text
- View/download PDF
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