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The electro-oxidation of the benomyl fungicide was studied by square-wave adsorptive stripping voltammetry. The voltammetric current at a glassy carbon electrode was acquired within the pH range 1.0-10.0. The quantitation was performed using the peak generated at +1144 mV by scanning the potential from +0.00 to +1600 mV (versus an Ag/AgCl reference electrode, 3 M NaCl). Accumulation potential = 0.0 mV, accumulation time = 45 s, frequency = 75 Hz, pulse amplitude = -60 mV, and staircase step potential = 7 mV were used as square-wave parameters. The peak current versus concentrations plot were rectilinear over the range from 0.081 to 1.496 µg/mL with an LOD of 0.024 µg/mL. Mean recovery was 99.0% (0.198 ± 0.011 µg/mL), which was very close to the benomyl content spiked into river water (0.20 µg/mL). The method was efficiently applied for benomyl determination in the pesticide formulation Minelate 50WG®, and the average determined content of 49.8 ± 0.16 (n = 5) was consistent with the 50% benomyl (w/w) quoted by the manufacturer. The benomyl voltammograms recorded between days exhibited a negligible degradation into carbendazim metabolite, and therefore all results were given as the total benomyl concentration. The high recoveries and low RSD gave evidence of good accuracy and precision. [ABSTRACT FROM AUTHOR]

Thirteen flavanone racemates were successfully separated using a Chiralpak® IA column and isopropanol-hexane (50:50, v/v). The mobile phase flow rate and detection wavelength were 0.5 mL/min and 254 nm. The retention times values ranged from 5.50 and 56.45 min. The values of the retention, separation, and resolution factors ranged from 0.63 to 21.67, 1.12 to 2.45, and 0.13 to 11.94. The docking binding energies ranged from -6.2 to -8.2 kcal/mol, showing enthalpy-determined host-guest complex formation. The molecular docking results and the experimental data were agreed well. The results showed that S-enantiomers had stronger bindings with chiral selectors compared to R-enantiomers. Consequently, the R-enantiomers eluted first followed by S-enantiomers. The reported method is highly useful to determine the enantiomeric composition of the reported flavanone in any sample., (© 2024 John Wiley & Sons Ltd.)

Due to a great demand for amylose and cellulose polymeric chromatographic chiral columns, the enantiomeric separation of thiourea derivatives of naringenin was achieved on the different amylose (Chiralpak-IB) and cellulose chiral (Chiralcel-OJ and Chiralcel-OD-3R) columns with varied chromatographic conditions. The isocratic mobile phases used were ethanol and methanol, where ethanol/hexane and methanol/hexane were used as gradient mode and were prepared in volume/volume relation. The separation and resolution factors for all the enantiomers were in the range of 1.25 to 3.47 and 0.48 to 1.75, respectively. The enantiomeric resolution was obtained within 12 min making fast separation. The docking studies confirmed the chiral recognition mechanisms with binding affinities in the range of -4.7 to -5.7 kcal/mol. The reported compounds have good anticoagulant activities and may be used as anticoagulants in the future. Besides, chiral separation is fast and is useful for enantiomeric separation in any laboratory in the world., (© 2024 Wiley Periodicals LLC.)

Antibiotics are considered as the new generation water pollutants as these disturb endocrine systems if water contaminated with antibiotics is consumed. Among many antibiotics norfloxacin is present in various natural water bodies globally. This antibiotic is considered an emerging pollutant due to its low degradation in aquatic animals. Besides, it has many side effects on human vital organs. Therefore, the present article discusses the recent advances in the removal of norfloxacin by adsorption. This article describes the presence of norfloxacin in natural water, consumption, toxicity, various adsorbents for norfloxacin removal, optimization factors for norfloxacin removal, kinetics, thermodynamics, modeling, adsorption mechanism and regeneration of the adsorbents. Adsorption takes place in a monolayer following the Langmuir model. The Pseudo-second order model represents the kinetic data. The adsorption capacity ranged from 0.924 to 1282 mg g -1 . In this sense, the parameters such as the NFX concentration added to the adsorbent textural properties exerted a great influence. Besides, the fixed bed-based removal at a large scale is also included. In addition to this, the simulation studies were also discussed to describe the adsorption mechanism. Finally, the research challenges and future perspectives have also been highlighted. This article will be highly useful for academicians, researchers, industry persons, and government authorities for designing future advanced experiments., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests. Environmental remediation of the norfloxacin in water by adsorption: Advances, current status and prospects, (Copyright © 2023. Published by Elsevier B.V.)

Heliotropium bacciferum plant is very important and has been used for medicinal values since ancient times. The present study deals with the extraction of essential oil (0.14 w/w) of this plant and its GC-MS analysis, anti-inflammatory, and anti-bacterial activities evaluation. GC analysis of essential oil confirmed 76 compounds with 49 terpenes and oxygenated hydrocarbons. The main components of this essential oil are agarospirol (15.15 %), rosifoliol (9.41 %), elemol (8.96 %), tau.-cadinol (8.05 %), linalool (5.37 %), shyobunol (5.36 %) cyclohexylmethyl tridecyl ester of oxalic acid (4.74 %), respectively. The essential oil showed quite good anti-inflammatory activity while this oil indicated excellent anti-bacterial activity against 3 g-positive pathogenic bacteria (Bacillus subtilusATCC 6633, Entrococcus faecalis ATCC 29212, and Bacillus cereusATCC 11778). The essential oil of Heliotropium bacciferum showed great potential as an anti-inflammatory and anti-bacterial agent. Therefore, the reported extraction and GC-MS analysis methods are useful to extract the essential oil and determine the composition of other plants. The extracted essential oil may be used to treat several diseases., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Heliotropium bacciferum essential extrcation: Compositions determination by GC-MS and anti-inflammatory and antibacterial activities evaluation., (Copyright © 2023. Published by Elsevier Inc.)

A polymer-based nanosensor and electrochemical methods were developed for the quantitative analysis of vanillin. The sample preparation was done using nano solid phase micro membrane tip extraction (NSPMMTE). A novel poly(phenylalanine)/TiO 2 /CPE sensor was built as the working electrode for the first time for the analysis of the vanillin substance. The electrochemical behavior and analytical performance of vanillin were examined in detail by cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV) techniques via the oxidation process. The optimized modules of the DPSV technique that affected the vanillin peak current and peak potential were pH, pulse amplitude, step potential, and deposition time. The electroactive surface areas of bare CPE, TiO 2 /CPE, and poly(phenylalanine)/TiO 2 /CPE electrodes were found to be 0.135 cm 2 , 0.155 cm 2 , and 0.221 cm 2 , respectively. The limit of detection (LOD) was 32.6 μg/L in the 0.25-15.0 mg/L working range at pH 7.0. The selectivity of the proposed DPSV method for the determination of vanillin on the modified electrode was investigated in the presence of various organic and inorganic substances, and the determination of vanillin with high recovery was achieved with less than 5% relative error. The analytical application was applied in chocolate samples and the DPSV method was found highly efficient, reproducible, and selective., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Coffee is a complex mixture of chemicals, which provide biologically active compounds with various health benefits. The some biologically active compounds arising from both its natural structure and formed after processing were determined as an antioxidant capacity of coffee beverages. In this study, we aimed to determine how roasting levels of Arabica coffee seed (light, medium, dark) and three brewing techniques-decoction methods (Turkish coffee), infusion method (filter coffee) and pressure methods (Espresso)-affect total antioxidant capacity in a cup of coffee beverage by electrochemical methods such as square wave stripping voltammetry (SWSV), differential pulse stripping voltammetry (DPSV) and cyclic voltammetry (CV). Antioxidant capacities of the coffee samples in terms of the equivalent amounts were determined according to standard oxidation peaks of rutin and caffeic acid. The highest antioxidant capacity was found in espresso coffee prepared at light roasting coffee seeds as equivalent the routine and caffeic at 9.4 ± 0.2 g/L and 19.7 ± 0.7 g/L, respectively with SWSV on a carbon paste electrode. As a result, SWSV, DPSV and CV voltammetric methods, fast, reliable, fully validated and without any pretreatment are alternative to conventional analytical methods to evaluation antioxidant values in any food samples., Competing Interests: Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare no conflict of interest., (© Association of Food Scientists & Technologists (India) 2022.)

Mepivacaine, an amide-type local anesthetic drug widely used in regional anesthesia, was studied by some aspects such as electrochemical behavior, mechanism illumination, and analytical applications by cyclic voltammetry (CV) and different pulse voltammetry (DPV) methods. In this study, a novel, fast, simple, portable, and the inexpensive electrochemical sensor was developed for the determination of mepivacaine. This study was carried out by mepivacaine anodic direction detection for the first time. The modified sensor was fabricated with silver nanoparticles (AgNP) and multiwalled carbon nanotubes paste electrode (MWCNTPE) by using the drop-dry method. Different experimental parameters, such as pulse amplitude, step potential, and scanning rate in the DPV application module, were optimized. Under optimal operation conditions, the limit of detection (LOD) as low as 31 μg L -1 was found over the dynamic range (0.1-8.0 mg L -1 ). In contrast to its high response towards mepivacaine, the DPV exhibits negligible responses on modified AgNP/MWCNTPE when exposed to interfering species such as dopamine, uric acid, glucose, ascorbic acid, and some heavy metals. Exceptionally, the proposed DPV method on modified AgNP/MWCNTPE was successfully applied to pharmaceutical dosage form and synthetic human serum with a low relative standard deviation (RSD) of 1.35% and 2.02%, respectively., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Brown HT and carmoisine, which are the most used dyestuffs in pharmaceuticals, textiles, cosmetics and foods, are important components of the Azo family. Although the Azo group is not toxic or carcinogenic under normal conditions, these dyestuffs require great care due to the reduction of the Azo functional group to amines. In particular, fast, reliable, easy, on-site and precise determinations of these substances are extremely necessary and important. In this review, the properties, applications, and electrochemical determinations of brown HT and carmoisine, which are used as synthetic food colorants, are discussed in detail. Up to now, sensor types, detection limits (LOD and LOQ), and analytical applications in the developed electrochemical strategies for both substances were compared. In addition, the validation parameters such as the variety of the sensors, sensitivity, selectivity and electrochemical technique in these studies were clarified one by one. While the electrochemical techniques recommended for brown HT were mostly used for the removal of dyestuff, for carmoisine they included fully quantitative centered studies. The percentiles of voltammetric techniques, which are the most widely used among these electroanalytical methods, were determined. The benefits of a robust electrochemical strategy for the determination of both food colors are summed up in this review. Finally, the brown HT and carmoisine suggestions for future perspectives in electrochemical strategy are given according to all their applications., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

This study provides a comprehensive review of the latest developments in the electrochemical impressions of the important dyestuffs including amaranth and carminic acid. Food colors are organic substances that have important effects on human health and food safety. While these substances do not pose a problem when used in the daily intake (ADI) amounts, they harm human health when consumed excessively. Amaranth and carminic acid are synthetic and natural food colors ingredients, respectively. Analysis of these substances in food, pharmaceutical, cosmetic and textile samples is extremely important because of their genotoxicity, cytostatic and cytotoxic effects. Electroanalytical methods, which have great advantages over traditional analytical methods, shed light on the scientific world. Electrochemical monitoring modules, which are fast, simple, accurate, reliable, and highly selective, are promising for the determination of both substances. Until now, amaranth and carminic acid food determinations have been carried out successfully with electrochemical monitoring techniques in many numbers in the literature. Voltammetric techniques are the most widely used among these electroanalytical methods. In particular, square wave and differential pulse voltammetric techniques, which have extraordinary properties, have been heavily preferred. Limits of detection (LOD) comparable to the standard analytical method have been achieved using these methods, which have very quick analysis durations, high precision and accuracy, do not require long preprocessing, and have great selectivity. In addition, more sensitive and selective analyses of amaranth and carminic acid in natural samples were carried out with numerous indicator electrodes. The merits of powerful electrochemical monitoring studies for the determination of both food colors during the last decade are presented in this study. Moreover, parameters such as analytical applications, detection limits, electrochemical methods, selectivity, working electrodes, and working ranges are summarized in detail., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Increasing evidence suggests that diabetes also targets lung tissues resulting in structural and physiological abnormalities. The present study evaluated the impact of pristine C60 fullerene (C60) against diabetes-induced lung damage for the first time. The objective was to evaluate the impacts of Curcumin (Cur), C60 and C60 fullerene+Curcumin (C60 + Cur) combination on oxidative stress (MDA, GSH, CAT, GST, Retinol, α-tocopherol), apoptosis (Caspase-3, Bcl-2), cholesterol and fatty acid profile (16:0,18:0,18:1,18:2, 22:4, 22:6) against changes in the lung tissue of diabetic rats. Streptozotocin (STZ) was used for inducing diabetes with Cur, C60 and C60 + Cur combination administered for eight weeks to treat diabetic and control rats. Increased oxidative stress, apoptosis and significant changes in cell structure were observed in the lung tissues of diabetic rats. The combination of Cur, C60 and C60 + Cur reduced oxidative stress in the lung tissue of diabetic rats while increasing the antioxidant defense capacity of the tissue, exhibiting tissue protective properties against apoptosis. The diabetic rats displayed favorable properties against lipotoxicity-induced tissue damage due to the increase in the fatty acid and cholesterol levels in lung tissue. It was observed that Cur, C60 and C60 + Cur combination displays protective effects against hyperglycemia induced oxidative damage to lung tissue. Oxidative stress, prevention of lipid and cholesterol accumulation, and weakening of lung apoptosis may be associated with these effects.

The present study aims to examine the protective effects of C60 fullerene (C60), Curcumin (CUR; Curcuma longa), C60 + CUR combination against oxidative stress, apoptosis, and changes in cellular level in kidney tissue of diabetic rats. Treatment practices were administered separately to groups for 8 weeks following the approval of diabetes induction. It was observed that the treatment groups had increased antioxidant potential, decreased oxidative stress levels, decreased cholesterol, alpha tocopherol, retinol levels along with improved important changes in fatty acid metabolism compared with the diabetic group. C60, CUR, and C60 + CUR were also determined to act in the direction of reducing kidney damage by activating apoptotic pathways. It can be concluded based on these findings that C60, CUR, and especially C60 + CUR combination has beneficial properties in maintaining kidney tissue and function by effectively preventing oxidative stress, apoptotic changes, and changes at the cellular level in kidney tissue under hyperglycemia conditions. PRACTICAL APPLICATIONS: C60 and CUR have various biological activities which can be indicated as antioxidant, anti-inflammatory, anticancer, neuroprotective, and hepatoprotective. It has been reported that C60 and CUR protect the cells against oxidative injury brought about by reactive oxygen species (ROS). Data acquired from the present study puts forth that C60 and C60 + CUR may be promising agents to prevent damage induced by hyperglycemic conditions in kidney tissue., (© 2020 Wiley Periodicals LLC.)

Diabetes mellitus is a prevalent metabolic disorder associated with multiple complications including neuropathy, memory loss and cognitive decline. Despite a long history of studies on diabetic complications, there are no effective therapeutic strategies for neuroprotection in diabetes. Hyperglycemia-induced imbalance in programmed cell death could initiate a decline in neural tissue cells viability. Various nanomaterials can induce either cell death or cell survival dependent on the type and surface features. Pristine C 60 fullerene is a nontoxic nanomaterial, which exhibits antioxidant and cytoprotective properties. However, the precise molecular mechanism with which the C 60 nanoparticle exerts cytoprotective effect in diabetic subjects has not yet been fully addressed. Thus, this study aimed to determine whether C 60 fullerene prevents oxidative stress impairment and to explore the effects of C 60 fullerene on apoptosis and autophagy in diabetes mellitus to clarify its potential mechanisms. These effects have been examined for olive oil extracted C 60 fullerene on the hippocampus of STZ diabetic rats. Up-regulation of Caspase-3, Beclin-1 and oxidative stress indexes and down-regulation of Bcl-2 were observed in the brain of STZ-diabetic rats. The exposure to C 60 fullerene for a period of 12 weeks ameliorate redox imbalance, hyperglycemia-induced disturbances in apoptosis and autophagy flux via modulation of Caspase-3, Bcl-2, Beclin-1 and LC3I/II contents. Furthermore, C 60 fullerene ameliorated the LC3I/II ratio and prevented extremely increased autophagy flux. Contrarily, pristine C 60 fullerene had no modulatory effect on all studied apoptotic and autophagy markers in non-diabetic groups. Therefore, oil extracted C 60 fullerene exhibits cytoprotective effect in hyperglycemia-stressed hippocampal cells. The presented results confirm that pristine C 60 fullerene nanoparticles can protect hippocampal cells against hyperglycemic stress via anti-oxidant, anti-apoptotic effects and amelioration of autophagy flux. Moreover, C 60 fullerene regulates a balance of autophagy via BCL-2/Beclin-1 reciprocal expression that could prevent functional disturbances in hippocampus.

Proparacaine, one of the most common local anesthetics to facilitate diagnosis and treatment of eye diseases, was assayed by square wave voltammetry using a paste electrode prepared with carbon nanotubes. In cyclic voltammetric studies, proparacaine has exhibited a single irreversible anodic peak at around + 900 mV vs. Ag/AgCl in pH 6.0 Britton-Robinson buffer solution. It was suggested that the peak had appeared due to the oxidation of the NH 2 group on the proparacaine molecule. Prior to the determination of the proparacaine by square wave stripping voltammetry (SWSV) on the fabricated multi-walled carbon nanotube paste electrode (MWCNTPE), the accumulation potential (E acc ), accumulation time (t acc ), pulse amplitude (ΔE), step potential (ΔE s ) and frequency (f ) parameters were optimized. The peak currents plotted in the range of 0.5 - 12.5 mg/L proparacaine exhibited two linear sections with a detection limit of 0.11 mg/L. The results for the determination of proparacaine on a pharmaceutical local anesthetic (Alcaine ® ) showed that relative standard deviation (RSD) and relative error (RE) were 4.1 and -2.0%, respectively. Selectivity has also been investigated and results showed recoveries of 5.0 mg/L proparacaine in the presence of 5.0 mg/L dopamine, ascorbic acid and uric acid as 106.9 ± 0.8, 99.9 ± 1.2 and 94.1 ± 0.7, respectively.