Search

A simple but efficient algorithm has been developed for computer analysis of eye tracking movements. The program separates smooth pursuit and saccadic eye movements. Separation of the two components is achieved using a two-step process of saccade detection. First, an AR model of the velocity of the smooth component is identified and used to determine a Kalman filter. Secondly the innovation sequence generated by this filter allows saccade detection. The precise beginning and end of each saccade are found using a Hinkley algorithm. Examples are given of analysis procedure for eye tracking of a random moving target. The method proved to be highly reliable and could be easily extended to other eye movements such as nystagmus.

P-glycoprotein (P-170) is the phenotypic marker of tumoral cells that show the phenomenon of multidrug resistance (MDR). Using an immunocytochemical approach, we employed the monoclonal antibody C219 (which recognizes an epitope of such a glycoprotein) to evaluate in cytologic samples the expression of P-170 on neoplastic cells from 52 patients affected by different hematologic malignancies and its eventual correlation to clinical outcome. Longitudinal studies were also performed in 14 patients. Results obtained demonstrated that a) the so-called "MDR phenotype" may be heterogeneously represented (from less than 1 to 100% of positive cells) in hemopoietic tumors at diagnosis (without exposure to pharmacologic agents), as well as during the course of the disease, although a more substantial presence of P-170 occurred in treated patients. There was no correlation between neoplastic kinetic activity (such as expression of Ki 67 recognized nuclear proliferation-associated antigen) and P-170-positive cells. b) Percentage of positive cells as well as intensity of staining seemed to be important in determining MDR; in general, there was a strong correlation between expression of P-170 in more than 20% of neoplastic cells and a lack of response to chemotherapy. However, some false-positive and false-negative cases were observed. c) The detection of scattered P-170-positive cells may predict a pharmacologic selection of intrinsic or mutant-resistant clones.