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The optimum experimental condition for simultaneous UV-spectrophotometric determination of the contents of aspirin, phenacetin and caffeine in aspirin compound tablets (APC) and the basic principle and application of partial least squares method(PLS) in simultaneous multicomponent determination have been studied. Confidence intervals of the three components are 100.1 +/- 0.23% (aspirin), 100.0 +/- 0.25% (phenacetin) and 100.1 +/- 0.33% (caffeine) (confidence 95%). No information has ever been available in the literature for the application of PLS in pharmaceutical analysis. Compared with other traditional computing methods, PLS is a more perfect multicomponent determination method. It is especially applicable to analyzing samples in batches. It is faster and produces more accurate and reliable results. PLS provides a new method for in-line UV-visible spectrophotometric automation.

Laser irradiation offers the potential of removing atherosclerotic plaques from blood vessels. We used the Nd:YAG laser to treat coronary atherosclerotic obstruction in patients undergoing coronary bypass. Of the 7 patients treated, all were successfully recanalized without damage to the artery wall (a mild hematoma developed in 1 case), and follow-up examination showed patency 4 months after operation. We conclude that Nd:YAG laser shows promise for the treatment of atherosclerotic obstructions, but more study is needed before wide clinical application is justified.

Monoclonal antibodies which react specifically with the nuclei of interphase cells recognized three nuclear antigens with molecular weights of 110,000 (p110), 85,000 (p85), and 18,000 (p18). p110 and p85 were found in eight tumor cell lines but were not found in resting lymphocytes. p18 was found in resting lymphocytes as well as the tumor cell lines. Protein p85 appeared in phytohemagglutinin-stimulated lymphocytes in the G1 phase and protein p110 appeared in the S phase. p110 and p85 were localized to the extranucleolar chromatin while p18 was distributed throughout the nucleus and was determined by microscopic and DNase digestion studies to be DNA associated. The anti-p110 antibody recognized a component of the DNA polymerase alpha 2 complex. Three novel nuclear proteins were identified using monoclonal antibodies. Two of these proteins (p110 and p85) are proliferating cell nuclear and nucleolar antigen-like while the third (p18) is not cell cycle dependent.