191 results on '"Almeida, C. Marisa R."'
Search Results
152. Influence of the Sea Rush Juncus maritimus on Metal Concentration and Speciation in Estuarine Sediment Colonized by the Plant
- Author
-
Almeida, C. Marisa R., primary, Mucha, Ana P., additional, and Vasconcelos, M. Teresa S. D., additional
- Published
- 2004
- Full Text
- View/download PDF
153. Multielement Composition of Wines and Their Precursors Including Provenance Soil and Their Potentialities As Fingerprints of Wine Origin
- Author
-
Almeida, C. Marisa R., primary and Vasconcelos, M. Teresa S. D., additional
- Published
- 2003
- Full Text
- View/download PDF
154. Lead Contamination in Portuguese Red Wines from the Douro Region: from the Vineyard to the Final Product
- Author
-
Almeida, C. Marisa R., primary and Vasconcelos, M. Teresa S. D., additional
- Published
- 2003
- Full Text
- View/download PDF
155. SPE sample pre-treatment using a mixed-mode sorbent of reverse-phase and ionic exchange for determination of ALMWOAs in waters.
- Author
-
Rocha, A. Cristina S., Almeida, C. Marisa R., Basto, M. Clara P., and Vasconcelos, M. Teresa S.D.
- Subjects
- *
MARSH plants , *MOLECULAR weights , *ORGANIC acids , *SORBENTS , *ION exchange (Chemistry) , *INFRARED array detectors , *MALEIC acid - Abstract
Studies concerning the role of aliphatic low molecular weight organic acids (ALMWOAs) in physical, chemical and biological processes occurring in rhizosphere have been carried out over the last decades. However, the analytical determination of ALMWOAs concentrations in nature is difficult owing to their considerably low levels and the interference of most environmental matrices, which demands pre-concentration steps. For these reasons, a pre-treatment of the sample prior to its analysis is usually required. Herein, a solid phase extraction (SPE) method is given as a sample pre-treatment step for ALMWOAs determination in solutions with plant exudates. A SPE sorbent, with characteristics different from those used until now, was used to overcome matrix interference. High-performance liquid chromatography (HPLC) coupled with a diode array detector (DAD) was used for analysis. Acceptable ALMWOAs recovery percentages were achieved for freshwater matrices and interference of trace metals (Cu, Cd and Ni) was not observed. The developed method was applied to identify and estimate the levels of ALMWOAs released in the solution (freshwater enriched with 10 mg/L Cu, 5 mg/L Ni and 1 mg/L Cd) where roots ofHalimione portulacoidesandPhragmites australisspecimens have been exposed, for 2 hours. Both plants liberated measurable amounts of oxalic acid andP. australisalso released into the solution citric and maleic acids. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
156. UV-irradiation and MW-digestion pre-treatment of Port wine suitable for the determination of lead isotope ratios by inductively coupled plasma mass spectrometry
- Author
-
Almeida, C. Marisa R., primary and Vasconcelos, M. Teresa S. D., additional
- Published
- 1999
- Full Text
- View/download PDF
157. Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean).
- Author
-
Tomasino, Maria Paola, Aparício, Mariana, Ribeiro, Inês, Santos, Filipa, Caetano, Miguel, Almeida, C. Marisa R., de Fátima Carvalho, Maria, and Mucha, Ana P.
- Subjects
MICROBIAL communities ,MARINE sediments ,SODIUM acetate ,MICROBIAL diversity ,OIL spills ,OCEAN - Abstract
Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising problematic environmental concerns. The present work aims to contribute to the study of DSS microbial resources as biotechnological tools for bioremediation of petroleum hydrocarbon polluted environments. Four deep-sea sediment samples were collected in the Mid-Atlantic Ridge, south of the Azores (North Atlantic Ocean). Their autochthonous microbial diversity was investigated by 16S rRNA metabarcoding analysis. In addition, a total of 26 deep-sea bacteria strains with the ability to utilize crude oil as their sole carbon and energy source were isolated from the DSS samples. Eight of them were selected for a novel hydrocarbonoclastic-bacterial consortium and their potential to degrade petroleum hydrocarbons was tested in a bioremediation experiment. Bioaugmentation treatments (with inoculum pre-grown either in sodium acetate or petroleum) showed an increase in degradation of the hydrocarbons comparatively to natural attenuation. Our results provide new insights into deep-ocean oil spill bioremediation by applying DSS hydrocarbon-degrading consortium in lab-scale microcosm to simulate an oil spill in natural seawater. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
158. The Role of a Salt Marsh Plant on Trace Metal Bioavailability in Sediments: Estimation By Different Chemical Approaches.
- Author
-
Almeida, C. Marisa R., Mucha, Ana P., and Vasconcelos, M. Teresa S. D.
- Subjects
SEDIMENTS ,METALS ,MARSHES ,PLANTS ,PHYSIOLOGICAL oxidation ,BIOAVAILABILITY - Abstract
The article presents information on the role of salt marsh plant on trace metal bioavailability in sediments. It says that Results and Discussion. Plants could concentrate metals around its roots and rhizomes. In addition, they were capable of oxidizing the anaerobic medium surrounding their roots in muddy sediment. As sulphide oxidation renders metals like Cadmium, Copper, Nickel and Lead into more soluble forms, according to the proposed model, metals from muddy sites would be more available in rhizosediment than in sediment.
- Published
- 2005
- Full Text
- View/download PDF
159. Does the winemaking process influence the wine 87Sr/86Sr? A case study
- Author
-
Almeida, C. Marisa R. and Vasconcelos, M. Teresa S.D.
- Subjects
- *
SOIL composition , *STRONTIUM , *ISOTOPES , *FORTIFIED wines - Abstract
To evaluate the roles of the soil composition and vinification process on the wine strontium isotope ratio 87Sr/86Sr, two different wines (one red table and one fortified wine) from the Douro Portuguese wine-district were selected. The ratio 87Sr/86Sr was determined in the provenance soil, in the grape juices (prepared in the laboratory) and in the wines. Total strontium concentration was determined in the grape juices, skins and seeds and in the samples collected in the different steps of each winemaking process and respective produced wines. All analyses were performed by inductively coupled plasma mass spectrometry. The total strontium concentration increased during the vinification processes. The increase was attributed to the element liberation from grape seeds and skins (richer in strontium than the pulp) during the initial mechanical pressing and fermentation. The 87Sr/86Sr values were statistically identical in the provenance soil, respective grape juice and wine, which indicates that 87Sr/86Sr is a suitable tracer of the origin of the studied wines. The study deserves to be extended to many more wines/provenance soils in order to validate the suitability of that parameter as a tool for the identification of wine provenance region. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
- View/download PDF
160. Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons.
- Author
-
Perdigão, Rafaela, Almeida, C. Marisa R., Santos, Filipa, Carvalho, Maria F., and Mucha, Ana P.
- Subjects
BIOREMEDIATION ,PETROLEUM ,CONSORTIA ,OIL spills ,SEDIMENTS ,ALIPHATIC hydrocarbons ,HYDROCARBONS - Abstract
Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera Pseudomonas, Rhodococcus and Acinetobacter. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
161. Assessment of Constructed Wetlands' Potential for the Removal of Cyanobacteria and Microcystins (MC-LR).
- Author
-
Bavithra, Guna, Azevedo, Joana, Oliveira, Flávio, Morais, João, Pinto, Edgar, Ferreira, Isabel M.P.L.V.O., Vasconcelos, Vitor, Campos, Alexandre, and Almeida, C. Marisa R.
- Subjects
MICROCYSTINS ,CONSTRUCTED wetlands ,MICROCYSTIS aeruginosa ,CYANOBACTERIAL blooms ,CYANOBACTERIA ,WATER purification - Abstract
Microcystis blooms and the subsequent release of hepatotoxic microcystins (MCs) pose a serious threat to the safety of water for human and livestock consumption, agriculture irrigation, and aquaculture worldwide. Microcystin-LR (MC-LR), the most toxic variant of MCs, has been widely detected in a variety of environments such as water, sediments, plants, and many aquatic organisms. Conventional solutions of water treatment are costly, requiring specific infrastructure, as well as specialized personnel and equipment. Therefore, these solutions are not feasible in many rural areas or in the treatment of large reservoirs. In this regard, low-cost and low-technology solutions, such as constructed wetlands (CWs), are attractive solutions to treat surface waters contaminated with toxic cyanobacteria blooms from lakes, ponds, reservoirs, and irrigation systems. In line with this, the main aim of this work was to evaluate the potential of CWs for the treatment of water contaminated with MC-LR produced by Microcystis aeruginosa—LEGE 91094. For that, microcosms (0.4 × 0.3 × 0.3 m) simulating CWs were assembled with Phragmites australis to treat lake water contaminated with Microcystis aeruginosa cells and MCs. Results showed removal percentages of M. aeruginosa cells above 94% and about 99% removal of MC-LR during 1 week treatment cycles. CWs maintained their functions, regardless the presence of MC-LR in the system, and also showed significant removal of nutrients (ammonium ion removal up to 86%) and organic matter (removal reaching 98%). The present work indicates that CWs have the potential for removal of cyanobacterial cells and cyanotoxins, which can be useful for the treatment of eutrophic waters and provide water of sufficient quality to be used, for instance, in agriculture. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
162. MarinEye - A tool for marine monitoring
- Author
-
Martins, Alfredo, Dias, Andre, Silva, Eduardo, Ferreira, Hugo, Dias, Ireneu, Almeida, Jose Miguel, Torgo, Luis, Goncalves, Marco, Guedes, Mauricio, Dias, Nuno, Jorge, Pedro, Mucha, Ana Paula, Magalhaes, Catarina, Carvalho, Maria Fatima, Ribeiro, Hugo, Almeida, C. Marisa R., Azevedo, Isabel, Ramos, Sandra, Borges, Teresa, Leandro, Sergio Miguel, Maranhao, Paulo, Teresa Mouga, Gamboa, Roberto, Lemos, Marco, Dos Santos, Antonina, Silva, Alexandra, Frazao E Teixeira, Brbara, Bartilotti, Catia, Marques, Raquel, Cotrim, Sonia, and IEEE
163. Methodological approaches for fractionation and speciation to estimate trace element bioavailability in engineered anaerobic digestion ecosystems: An overview
- Author
-
Luigi Frunzo, Jimmy Roussel, Gilles Guibaud, Giovanni Esposito, Eric D. van Hullebusch, Sepehr Shakeri Yekta, Markus Lenz, Cynthia Carliell-Marquet, Lars Duester, Gavin Collins, Ulf Skyllberg, Fernando G. Fermoso, Mirco Garuti, Markus Ortner, Rohan Jain, Stéphane Simon, Yoan Pechaud, Emmanuel Guillon, C. Marisa R. Almeida, Groupement de Recherche Eau, Sol, Environnement (GRESE), Université de Limoges (UNILIM), Laboratoire Géomatériaux et Environnement (LGE), Université Paris-Est Marne-la-Vallée (UPEM), Universidade do Porto, Università degli studi di Cassino e del Lazio Meridionale (UNICAS), CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, European Research Council, European Cooperation in Science and Technology, European Commission, van Hullebusch, Eric D., Guibaud, Gille, Simon, Stéphane, Lenz, Marku, Yekta, Sepehr Shakeri, Fermoso, Fernando G., Jain, Rohan, Duester, Lar, Roussel, Jimmy, Guillon, Emmanuel, Skyllberg, Ulf, Almeida, C. Marisa R., Pechaud, Yoan, Garuti, Mirco, Frunzo, Luigi, Esposito, Giovanni, Carliell Marquet, Cynthia, Ortner, Marku, Collins, Gavin, and Universidade do Porto = University of Porto
- Subjects
dissolved organic-matter ,Sequential fractionation ,trace elements ,010501 environmental sciences ,Bacterial growth ,01 natural sciences ,Biochemistry ,Analytical method ,Methodological approach ,Physicochemical reactions ,Fractionation ,Waste Management and Disposal ,Sulfur compounds ,sequential extraction procedure ,ComputingMilieux_MISCELLANEOUS ,Water Science and Technology ,media_common ,heavy-metal speciation ,Ecology ,Heavy metals ,Organic macromolecules ,Pollution ,size-exclusion chromatography ,Environmental chemistry ,Milieutechnologie ,tank biogas reactors ,Anaerobic bioprocesses ,Biotechnology ,Environmental Engineering ,x-ray microscopy ,media_common.quotation_subject ,Complex networks ,plasma-mass spectrometry ,Biology ,Analytical methods ,Anaerobic digestion ,donnan membrane technique ,Ecosystem ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,Anaerobic digestion, analytical methods, bioavailability, fractionation, speciation, trace elements ,fractionation ,0105 earth and related environmental sciences ,Trace elements ,010401 analytical chemistry ,acid volatile sulfide ,Trace element ,0104 chemical sciences ,Bioavailability ,Speciation ,analytical methods ,speciation ,13. Climate action ,bioavailability ,Environmental Technology ,field-flow fractionation - Abstract
91 Páginas, 4 Tablas, 6 Figuras, Optimal supply of trace elements (TE) is a prerequisite for microbial growth and activity in anaerobic digestion (AD) bioprocesses. However, the required concentrations and ratios of essential TE for AD biotechnologies strongly depend on prevailing operating conditions as well as feedstock composition. Furthermore, TE in AD bioreactors undergo complex physico-chemical reactions and may be present as free ions, complex bound or as precipitates depending on pH, or on the presence of sulfur compounds or organic macromolecules. To overcome TE deficiency various commercial mineral products are typically applied to AD processes. The addition of heavy metals poses the risk of overdosing operating systems, which may be toxic to microbial consortia and ultimately the environment. Adequate supplementation, therefore, requires not only appropriate knowledge about the composition, but also on the speciation and bioavailability of TE. However, very little is yet fully understood on this specific issue. Evaluations of TE typically only include the measurement of total TE concentrations but do not consider the chemical forms in which TE exist. Thus detailed information on bioavailability and potential toxicity cannot be provided. This review provides an overview of the state-of-the-art in approaches to determine bioavailable TE in anaerobic bioprocesses, including sequential fractionation and speciation techniques. Critical aspects and considerations, including with respect to sampling and analytical procedures, as well as mathematical modelling, are examined. The approaches discussed in this review are based on our experiences and on previously published studies in the context of the ‘COST Action 1302: European Network on Ecological Roles of Trace Metals in Anaerobic Biotechnologies'., This article is based upon work from COST Action 1302 (‘European Network on Ecological Roles of Trace Metals in Anaerobic Biotechnologies’) supported by COST (European Cooperation in Science and Technology). EvH and GE acknowledge support from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 643071 (‘Advanced Biological Waste-to-Energy Technologies – ABWET’). GC acknowledges funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme grant agreement No. 261330. The authors thank the two anonymous reviewers for their comments, which significantly improved the quality of the manuscript.
- Published
- 2016
164. Potential of nature-based solutions to reduce antibiotics, antimicrobial resistance, and pathogens in aquatic ecosystems. a critical review.
- Author
-
Pastor-López EJ, Escolà M, Kisielius V, Arias CA, Carvalho PN, Gorito AM, Ramos S, Freitas V, Guimarães L, Almeida CMR, Müller JA, Küster E, Kilian RM, Diawara A, Ba S, and Matamoros V
- Subjects
- Wetlands, Drug Resistance, Microbial, Waste Disposal, Fluid methods, Water Pollutants, Chemical analysis, Wastewater microbiology, Rivers microbiology, Rivers chemistry, Anti-Bacterial Agents, Ecosystem
- Abstract
This comprehensive scientific review evaluates the effectiveness of nature-based solutions (NBS) in reducing antibiotics (ABs), combating antimicrobial resistance (AMR), and controlling pathogens in various aquatic environments at different river catchment levels. It covers conventional and innovative treatment wetland configurations for wastewater treatment to reduce pollutant discharge into the aquatic ecosystems as well as exploring how river restoration and saltmarshes can enhance pollutant removal. Through the analysis of experimental studies and case examples, the review shows NBS's potential for providing sustainable and cost-effective solutions to improve the health of aquatic ecosystems. It also evaluates the use of diagnostic indicators to predict NBS effectiveness in removing specific pollutants such as ABs and AMR. The review concludes that NBS are feasible for addressing the new challenges stemming from human activities such as the presence of ABs, AMR and pathogens, contributing to a better understanding of NBS, highlighting success stories, addressing knowledge gaps, and providing recommendations for future research and implementation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
165. Metformin and lamotrigine sorption on a digestate amended soil in presence of trace metal contamination.
- Author
-
Baldasso V, Sayen S, Gomes CAR, Frunzo L, Almeida CMR, and Guillon E
- Subjects
- Soil, Lamotrigine, Anticonvulsants, Adsorption, Pharmaceutical Preparations, Metals, Heavy analysis, Metformin, Soil Pollutants analysis, Trace Elements
- Abstract
The antidiabetic drug metformin and antiepileptic drug lamotrigine are contaminants of emerging concern that have been detected in biowaste-derived amendments and in the environment, and their fate must be carefully studied. This work aimed to evaluate their sorption behaviour on soil upon digestate application. Experiments were conducted on soil and digestate-amended soil as a function of time to study kinetic processes, and at equilibrium also regarding the influence of trace metals (Pb, Ni, Cr, Co, Cu, Zn) at ratio pharmaceutical/metal 1/1, 1/10, and 1/100. Pharmaceutical desorption experiments were also conducted to assess their potential mobility to groundwater. Results revealed that digestate amendment increased metformin and lamotrigine adsorbed amounts by 210% and 240%, respectively, increasing organic matter content. Metformin adsorption kinetics were best described by Langmuir model and those of lamotrigine by Elovich and intraparticle diffusion models. Trace metals did not significantly affect the adsorption of metformin in amended soil while significantly decreased that of lamotrigine by 12-39%, with exception for Cu
2+ that increased both pharmaceuticals adsorbed amounts by 5 - 8%. This study highlighted the influence of digestate amendment on pharmaceutical adsorption and fate in soil, which must be considered in the circular economy scenario of waste-to-resource., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
166. Spatial distribution and temporal trends of butyltin compounds (TBT, DBT & MBT) in short sediment cores of the SW Portuguese Shelf (western Iberian Margin, NE Atlantic).
- Author
-
Mil-Homens M, Almeida CMR, Dias S, Soares W, van Gaever P, de Stigter H, Santos MM, Santana A, Freitas M, Abrantes F, and Caetano M
- Abstract
Spatial patterns and temporal trends of the butyltin compounds tributyltin (TBT), dibutyltin (DBT), and monobutyltin (MBT) were investigated in a set of sediment samples collected along the SW Portuguese continental shelf. This region did not reach the Good Environmental Status (GES) in accordance with the Marine Strategy Framework Directive (MSFD) during a first evaluation carried out in 2012. Overall, MBT and DBT were the predominant organotin species detected, but high concentrations of TBT were found in and around disposal sites for dredge sludge derived from the dredging in navigation channels, harbours, and shipyard facilities of the Tagus and Sado estuaries. Although Portuguese regulations for monitoring sediment quality in relation to dredging activities consider only PAH, PCB and HCB, they also dictate that other organic contaminants such as butyltin compounds (BTs) should be monitored if suspicion of high values exists, but no action limits are defined for these (MAOTDR, 2007). Without action limits, the monitoring recommendation given in the regulations is not put into practice. Considering their toxicity, BT derivates should be integrated in the legislation, because they represent an environmental threat in the relocation of dredged material, especially when derived from harbour and shipyards areas. Based on this study, we recommend giving more attention to the amounts and impacts of BTs in sediments at dredged material disposal sites (DMDS) and their surroundings. Or even better, in order to be more efficient, monitoring should be done at the source of the dredged materials and not at the sink. In case it is not done, the monitoring of concentrations of TBT (and other BTs) in sediments and organisms, including imposex studies, at all Portuguese sites for disposal of dredged material receiving slightly to strongly contaminated dredged material must be developed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationship that could have appeared to influence the work reported in this manuscript., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
167. Methodology optimization to quantify microplastic presence in planktonic copepods, chaetognaths and fish larvae.
- Author
-
Rodrigues SM, Espincho F, Elliott M, Almeida CMR, and Ramos S
- Abstract
Two of the groups most impacted by microplastics (MPs) are zooplankton and fish larvae, either through MPs ingestion or absorption. Although there has been an increase of studies focusing on MPs ingestion by these organisms, there is still no standardized methodology for the quantification of MPs present in plankton. For example, some reagents normally used to digest plankton and recover MPs appear adversely to affect some plastic characteristics. This can potentially lead to underestimating the amount and types of MPs present in the organisms analyzed. Hence, this work aimed to optimize a methodology to quantify MPs present in plankton, namely zooplankton and fish larvae, and ensuring MPs integrity. Hence, the planktonic organism tissues were digested using 30% (v/v) H
2 O2 solution at different temperatures and incubation periods while preserving the integrity and polymer characteristics of 13 types of MPs. MPs' characteristics were register before and after the tests, by visual inspection and Fourier Transform Infrared Spectroscopy (FTIR) analysis, to evaluate the integrity and features of MPs. With this methodology, MPs recovery was above 85% for all types of plastic tested. The proposed methodology is a rapid protocol, with a maximum of 7 h of incubation, that ensures simultaneously the full digestion of the organism tissues and the complete preservation of all the plastic characteristics, namely color, size and polymer type.•A methodology was optimized to quantify microplastics present in zooplankton (copepods, chaetognaths and fish larvae).•Thirteen types of microplastics (fibers and fragments of different polymers) were used to test the efficiency of the methodology ensuring the maintenance of the integrity of plastics.•With this methodology, microplastic recovery was above 85% for all the types of microplastic tested and no changes in their characteristics were observed., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors. Published by Elsevier B.V.)- Published
- 2023
- Full Text
- View/download PDF
168. Tailoring of Mesoporous Silica-Based Materials for Enhanced Water Pollutants Removal.
- Author
-
Flores D, Almeida CMR, Gomes CR, Balula SS, and Granadeiro CM
- Abstract
The adsorptive performance of mesoporous silica-based materials towards inorganic (metal ions) and organic (dyes) water pollutants was investigated. Mesoporous silica materials with different particle size, surface area and pore volume were prepared and tailored with different functional groups. These materials were then characterised by solid-state techniques, namely vibrational spectroscopy, elemental analysis, scanning electron microscopy and nitrogen adsorption-desorption isotherms, allowing the successful preparation and structural modifications of the materials to be confirmed. The influence of the physicochemical properties of the adsorbents towards the removal of metal ions (Ni
2+ , Cu2+ and Fe3+ ) and organic dyes (methylene blue and methyl green) from aqueous solutions was also investigated. The results reveal that the exceptionally high surface area and suitable ζ-potential of the nanosized mesoporous silica nanoparticles (MSNPs) seem to favour the adsorptive capacity of the material for both types of water pollutants. Kinetic studies were performed for the adsorption of organic dyes by MSNPs and large-pore mesoporous silica (LPMS), suggesting that the process follows a pseudo-second-order model. The recyclability along consecutive adsorption cycles and the stability of the adsorbents after use were also investigated, showing that the material can be reused. Current results show the potentialities of novel silica-based material as a suitable adsorbent to remove pollutants from aquatic matrices with an applicability to reduce water pollution.- Published
- 2023
- Full Text
- View/download PDF
169. Buried and surface oil degradation - Evaluating bioremediation to increase PAHs removal through linear mathematical models.
- Author
-
Stoichev T, Mucha AP, Bernabeu A, and Almeida CMR
- Subjects
- Humans, Biodegradation, Environmental, Sand, Linear Models, Soil Microbiology, Polycyclic Aromatic Hydrocarbons analysis, Petroleum analysis, Soil Pollutants analysis
- Abstract
A bioremediation approach with tide simulation for buried and surface oil degradation was tested for removal of two, three and four rings polycyclic aromatic hydrocarbons (PAHs). Linear models depicted degradation constants of individual PAH as simple additive function of their initial concentrations (C
0 ) in contaminated sand, hydrophobicity, sampling layer and treatment conditions. For all PAHs and treatment conditions, the degradation of oil in buried layers was faster than at the surface. Naturally-occurring microorganisms proved to be efficient for bioremediation of PAHs and were stimulated by fertilizer addition (biostimulation, BS). Bioaugmentation (BA) by addition of a slurry of a native oil-degraders pre-stimulated consortium did not show faster PAH degradation than BS. Degradation was more rapid for PAH present at low C0 and with intermediate hydrophobicity. Bioremediation of beach sand either with surface or buried crude oil is a cost-effective strategy to clean-up different hydrocarbon families, including persistent ones, such as PAHs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
170. Cadmium effects on net N2O production by the deep-sea isolate Shewanella loihica PV-4.
- Author
-
Pizarro L, Magalhães C, Almeida CMR, Carvalho MF, and Semedo M
- Subjects
- Cadmium toxicity, Denitrification, Shewanella genetics, Microbiota
- Abstract
Deep-sea mining may lead to the release of high concentrations of metals into the surrounding seabed, which can disturb important ecosystem functions provided by microbial communities. Among these, the production of N2O and its reduction to N2 is of great relevance since N2O is an important greenhouse gas. Metal impacts on net N2O production by deep-sea bacteria are, however, currently unexplored. Here, we evaluated the effects of cadmium (Cd) on net N2O production by a deep-sea isolate, Shewanella loihica PV-4. We performed a series of Cd exposure incubations in oxic conditions and determined N2O fluxes during induced anoxic conditions, as well as the relative expression of the nitrite reductase gene (nirK), preceding N2O production, and N2O reductase gene (nosZ), responsible for N2O reduction. Net N2O production by S. loihica PV-4 exposed to Cd was strongly inhibited when compared to the control treatment (no metal). Both nirK and nosZ gene expression were inhibited in reactors with Cd, but nirK inhibition was stronger, supporting the lower net N2O production observed with Cd. The Cd inhibition of net N2O production observed in this study poses the question whether other deep-sea bacteria would undergo the same effects. Future studies should address this question as well as its applicability to complex communities and other physicochemical conditions, which remain to be evaluated., (© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.)
- Published
- 2023
- Full Text
- View/download PDF
171. Removing chemical and biological pollutants from swine wastewater through constructed wetlands aiming reclaimed water reuse.
- Author
-
Bôto ML, Dias SM, Crespo RD, Mucha AP, and Almeida CMR
- Subjects
- Swine, Animals, Wastewater chemistry, Wetlands, Waste Disposal, Fluid methods, Water, Fertilizers, Bacteria, Environmental Pollutants, Water Pollutants, Chemical analysis
- Abstract
Reusing reclaimed wastewater is needed to fight water scarcity, reduce freshwater consumption and conserve water resources, but one must ensure that hazardous substances are fully removed/eliminate before that reuse. The potential of lab-scale constructed wetlands (CWs) for the removal of chemical and biological contaminants from livestock wastewater, while maintaining nutrient levels for fertilization, was assessed, evaluating changes in microbial communities, with particular focus on potential pathogens. CW microcosms with two different substrates (lava rock or light expanded clay aggregate), both planted with Phragmites australis, were tested. After 15 days of treatment, removal rates were higher than 80% for Cd, Cr, Cu, Fe, Pb and Zn, in general with no significant differences between the two different substrates. Organic matter and nutrients were also removed but their levels still allowed the used of the treated wastewater as a fertilizer Removal of bacterial contamination was estimated through enumeration of cultivable bacteria. High removal rates of fecal indicator bacteria were observed, reaching >95% for enterococci and >98% for enterobacteria after 15 days of treatment, decreasing hazardous biological contaminants initially present in the wastewater. In addition, the microbial communities in the initial and treated wastewater, and in the plant roots bed substrate, were characterized by using 16SrRNA gene amplicon sequencing. Microbial communities in the CW systems showed a clear shift comparatively with the initial wastewater showing system adaptation and removal potentialities. This also revealed an important removal of the most represented potential pathogenic genus, Clostridium, which relative abundance decreased from 33% to 1% through the treatment. Overall, CWs showed potential to be efficient in removing chemical and biological contaminants, while maintaining moderated levels of nutrients, allowing the reuse of reclaimed water in agriculture, namely as fertilizer. Current results will contribute for the optimization and use of CWs for a sustainable treatment of liquid wastes, promoting the circular economy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
172. Microplastic contamination in large migratory fishes collected in the open Atlantic Ocean.
- Author
-
Pereira R, Rodrigues SM, Silva D, Freitas V, Almeida CMR, and Ramos S
- Subjects
- Animals, Humans, Plastics, Environmental Monitoring, Atlantic Ocean, Fishes, Microplastics, Water Pollutants, Chemical analysis
- Abstract
Fishes are one of the most important components of the oceans and are exposed to several anthropogenic pressures, namely microplastic (MP), contaminants that are now ubiquitous worldwide. Taking advantage of the 2020 Circumnavigation Expedition carried by the NRP Sagres tall ship of the Portuguese Navy, fish samples from the southern Atlantic ocean were collected to evaluate possible MP contamination. In a total of 14 weeks of campaign, seven large migratory fishes of commercial interest were collected across the middle Atlantic Ocean and along the South American Atlantic coast. All individuals were contaminated with MPs, with an average of 18 ± 11 MPs/fish. In all fish sampled, both the gastrointestinal tract and gills presented MPs, indicating different contamination pathways including via their preys and from surrounding water, respectively. A total of 124 MPs were observed, where 72 % were fibers and 28 % particles, mostly of blue color (85 %), and with rayon and nylon as the most abundant polymers. This study is an important contribution to increase the scientific knowledge of MP contamination in mesopelagic fishes used for human consumption and collected in the open waters, reinforcing the need for further research regarding MP contamination in top predatory species from high trophic levels., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
173. Optimization of an Analytical Protocol for the Extraction of Microplastics from Seafood Samples with Different Levels of Fat.
- Author
-
Silva DM, Almeida CMR, Guardiola F, Rodrigues SM, and Ramos S
- Subjects
- Animals, Environmental Monitoring methods, Hydrogen Peroxide, Plastics, Polymers, Seafood analysis, Microplastics, Water Pollutants, Chemical analysis
- Abstract
Marine organisms are affected by the ubiquitous occurrence of microplastics (MPs) in the environment. Several protocols have been described to extract and quantify MPs in seafood, although their complex matrices, with high level of fat, can compromise the efficiency of MPs extraction. To solve this issue, the present study aimed to develop a detailed methodology suitable to process seafood samples with different levels of fat, namely fish and molluscs, from fresh and canned sources, including the immersive liquids from the cans. Sample digestion was tested using different solutions (10% KOH, 30% H
2 O2 ), temperatures (40 °C, 65 °C) and incubation times (24, 48, 72 h). For fat removal, three detergents (two laboratory surfactants and a commercial dish detergent) and 96% ethanol were tested, as well as the manual separation of fat. The methodology optimized in this study combined a digestion with 30% H2 O2 at 65 °C, during 24 to 48 h, with a manual separation of the fat remaining after the digestion. All steps from the present methodology were tested in six types of polymers (PE-LD, PET, PE, AC, PS, and lycra), to investigate if these procedures altered the integrity of MPs. Results showed that the optimized methodology will allow for the efficient processing of complex seafood samples with different fat levels, without compromising MPs integrity (recoveries rate higher than 89% for all the polymers tested).- Published
- 2022
- Full Text
- View/download PDF
174. Antibiotics removal from aquaculture effluents by ozonation: chemical and toxicity descriptors.
- Author
-
Gorito AM, Ribeiro ARL, Rodrigues P, Pereira MFR, Guimarães L, Almeida CMR, and Silva AMT
- Subjects
- Animals, Oxytetracycline toxicity, Ozone chemistry, Sulfadimethoxine toxicity, Sulfamethoxazole toxicity, Trimethoprim toxicity, Zebrafish, Anti-Bacterial Agents toxicity, Aquaculture, Water Pollutants, Chemical toxicity, Water Purification methods
- Abstract
Antibiotics are often applied in aquaculture to prevent fish diseases. These substances can cause disturbances on receiving waters, when not properly eliminated from the aquaculture effluents. In this work, ozone (O
3 ) was investigated as a possible oxidizing agent to remove fishery antibiotics from aquaculture effluents: florfenicol (FF), oxytetracycline (OTC), sulfadimethoxine (SDM), sulfamethoxazole (SMX), and trimethoprim (TMP). Batch experiments were performed using ultrapure water and aquaculture effluents spiked with a mixture of target antibiotics at relatively high concentrations (10 mg L-1 each). OTC, SMX and TMP were fully removed (< 30 min) regardless of the tested conditions, mainly by O3 direct attack. In contrast, FF was partially removed in 30 min (∼ 10 and 60%, in aquaculture effluents and ultrapure water, respectively), but only in the presence of hydroxyl radicals (HO• ), the FF concentrations reaching levels below the detection limits in ultrapure water after 60 min. In the case of SDM, its degradation was highly influenced by the selected water matrix, but with removals always higher than 68%. In continuous-flow experiments applying more environmentally relevant antibiotic concentrations (100 ng L-1 each) and low O3 doses (1.5 mg L- 1 ), ozonation highly removed (> 98%) all tested antibiotics from aquaculture effluents with a hydraulic retention time (HRT) of 10 min, except FF (68%). Although by-products were detected in treated samples, zebrafish (Danio rerio) embryotoxicity tests did not show a toxicity increase by applying this ozonation treatment. Ozonation is thus a possible solution to remove antibiotics from aquaculture effluents. Still, full-scale studies in aquaculture farms are needed, and generation of HO• may be favoured to readily oxidize the FF antibiotic., (Copyright © 2022. Published by Elsevier Ltd.)- Published
- 2022
- Full Text
- View/download PDF
175. Correction: Tomasino et al. Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean). Microorganisms 2021, 9 , 2389.
- Author
-
Tomasino MP, Aparício M, Ribeiro I, Santos F, Caetano M, Almeida CMR, de Fátima Carvalho M, and Mucha AP
- Abstract
The authors wish to make the following corrections to this paper [...].
- Published
- 2022
- Full Text
- View/download PDF
176. Advanced oxidation technologies and constructed wetlands in aquaculture farms: What do we know so far about micropollutant removal?
- Author
-
Gorito AM, Lado Ribeiro AR, Pereira MFR, Almeida CMR, and Silva AMT
- Subjects
- Animals, Aquaculture, Ecosystem, Humans, Prospective Studies, Waste Disposal, Fluid, Wastewater, Water Pollutants, Chemical analysis, Wetlands
- Abstract
Aquaculture is the fastest growing animal food-producing sector. Water is the central resource for aquaculture, and it is essential that its quality be preserved. Micropollutants (MPs) can reach aquaculture through anthropogenic addition or inlet water, and may cause harmful effects such as endocrine disruption and antibiotic resistance, adversely affecting the fish species being farmed. Furthermore, the discharge of aquaculture effluents into the environment may contribute to the deterioration of water courses. In this sense, the implementation of environmentally responsible measures in aquaculture farms is imperative for the protection of ecosystems and human health. The European Commission (EC) has recently launched a guiding document promoting ecological aquaculture practices; however, options for water treatment are still lacking. Conventional processes are not designed to deal with MPs; this review article consolidates relevant information on the application of advanced oxidation technologies (AOTs) and constructed wetlands (CWs) as potential strategies in this regard. Although 161 studies on the application of AOTs or CWs in aquaculture have already been published, only 34 focused on MPs (28 on AOTs and 6 on CWs), whereas the others reported the removal of contaminants such as bacteria, organic matter, solids and inorganic ions. No study coupling both treatments has been reported to date for the removal of MPs from aquaculture waters. AOTs and CWs are prospective alternatives for the treatment of aquacultural aqueous matrices. However, the type of aquaculture activity and the specifications of these available technologies should be considered while selecting the most suitable treatment option., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
177. Revisiting pesticide pollution: The case of fluorinated pesticides.
- Author
-
Alexandrino DAM, Almeida CMR, Mucha AP, and Carvalho MF
- Subjects
- Ecosystem, Environmental Pollution, Environmental Pollutants, Pesticides analysis, Water Pollutants, Chemical analysis
- Abstract
Fluorinated pesticides acquired a significant market share in the agrochemical sector due to the surge of new fluoroorganic ingredients approved in the last two decades. This growing trend has not been accompanied by a comprehensive scientific and regulatory framework entailing all their potential negative impacts for the environment, especially when considering the hazardous properties that may result from the incorporation of fluorine into organic molecules. This review aims to address the safe/hazardous dichotomy associated with fluorinated pesticides by providing an updated outlook on their relevancy in the agrochemical sector and how it leads to their role as environmental pollutants. Specifically, the environmental fate and distribution of these pesticides in the ecosystems is discussed, while also analysing their potential to act as toxic substances for non-target organisms., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
178. Microplastics as a vehicle of exposure to chemical contamination in freshwater systems: Current research status and way forward.
- Author
-
Eder ML, Oliva-Teles L, Pinto R, Carvalho AP, Almeida CMR, Hornek-Gausterer R, and Guimarães L
- Subjects
- Ecosystem, Environmental Monitoring, Fresh Water, Plastics toxicity, Microplastics, Water Pollutants, Chemical analysis, Water Pollutants, Chemical toxicity
- Abstract
Contamination by microplastics is increasing steadily worldwide, affecting all environments. Additionally, aquatic organisms are often exposed to mixtures of other contaminants, including various chemicals. Numerous studies reported adsorption of chemicals to microplastics, raising concern about their possible role as vehicles of exposure through transfer to biota. Nevertheless, until recently, the studies on the topic were mostly focused on the marine environment. In the past five years, however, plenty of publications contributed empirical data about freshwater ecosystems, raising the need for a critical appraisal of the information. Herein the scientific literature was reviewed and multivariate data analysis was done. The analysed studies employed widely different experimental designs, endpoints, test species, shapes and concentrations of various polymer types and chemicals, often not relevant for the freshwater environment. Our integrated analytical approach revealed unfathomable research gaps, given the theoretical knowledge available and lessons learned from research about the marine environment. Greater harmonization of laboratory studies investigating this topic is needed, as well as testing conditions reflecting real exposure scenarios. Furthermore, standardized testing protocols are urgently required to guide such experiments and improve the comparability of the results obtained., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
179. Microplastics and plankton: Knowledge from laboratory and field studies to distinguish contamination from pollution.
- Author
-
Rodrigues SM, Elliott M, Almeida CMR, and Ramos S
- Subjects
- Ecosystem, Environmental Monitoring, Laboratories, Plankton, Plastics, Microplastics, Water Pollutants, Chemical analysis, Water Pollutants, Chemical toxicity
- Abstract
Due to their ubiquitous presence, size and characteristics as ability to adsorb pollutants, microplastics are hypothesized as causing a major impact on smaller organisms, such as plankton. Despite this, there is a need to determine whether these impacts just relate to the environmental presence of the materials or their effects on biological processes. Therefore, we aimed to 1) review current research on plankton and microplastics; 2) compare field and laboratory experimental findings, and 3) identify knowledge gaps. The systematic review showed that 70% of the 147 relevant scientific publications were from laboratory studies and microplastics interactions with plankton were recorded in 88 taxa. Field study publications were relatively scarce and the characteristics of microplastics collected in the field were very different from those used in laboratory experiments thereby limiting the comparison between studies. Our systematic review highlighted knowledge gaps in: 1) the number of field studies; 2) the non-comparability between laboratory and field conditions, and 3) the low diversity of plankton species studied. Furthermore, this review indicated that while there are many studies on contamination by microplastics, the effects of this contamination (i.e., pollution per se) have been less well-studied, especially in the field at population, community, and ecosystem levels., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
180. Microbial degradation of two highly persistent fluorinated fungicides - epoxiconazole and fludioxonil.
- Author
-
Alexandrino DAM, Mucha AP, Almeida CMR, and Carvalho MF
- Subjects
- Biodegradation, Environmental, Sodium Acetate metabolism, Soil Microbiology, Water Purification methods, Epoxy Compounds metabolism, Fungicides, Industrial metabolism, Microbial Consortia physiology, Persistent Organic Pollutants metabolism, Triazoles metabolism, Water Pollutants, Chemical metabolism
- Abstract
Biodegradation of two highly persistent fluorinated fungicides, epoxiconazole (EPO) and fludioxonil (FLU), by microbial consortia enriched from estuarine sediment and agricultural soil is reported. After an enrichment period of 6 months, four microbial consortia were able to completely remove and defluorinate the fungicides in co-metabolic conditions. Defluorination was biologically mediated and results suggest it is not a primary catabolic step, as fungicide removal was always faster than its defluorination. Three of the four enriched consortia had similar biodegradation performances in the absence of a co-substrate. Biodegradation kinetics revealed that microbial degradation followed a first-order kinetics, with cultures being capable of biodegrading concentrations up to 10 mg L
-1 of EPO or FLU, in a maximum of 21 days. Estimated half-life values for these compounds were significantly lower than those reported in literature, highlighting the unique metabolic performance of the obtained consortia. Analysis of their microbial composition revealed that they integrate several bacterial species belonging to the Proteobacteria phylum, with the most common genera being Pseudomonas, Ochrobactrum and Comamonas. This is the first study providing clear evidence on the biodegradation of EPO and FLU, opening doors for the design of bioremediation technologies for the recovery of ecosystems polluted with such recalcitrant compounds., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
181. Seasonal effect in nutritional quality and safety of the wild sea urchin Paracentrotus lividus harvested in the European Atlantic shores.
- Author
-
Rocha F, Rocha AC, Baião LF, Gadelha J, Camacho C, Carvalho ML, Arenas F, Oliveira A, Maia MRG, Cabrita AR, Pintado M, Nunes ML, Almeida CMR, and Valente LMP
- Subjects
- Animals, Atlantic Ocean, Carotenoids analysis, Europe, Fatty Acids, Unsaturated analysis, Gonads chemistry, Gonads metabolism, Sea Urchins metabolism, Seasons, Nutritive Value, Paracentrotus metabolism
- Abstract
Paracentrotus lividus gonads are gastronomic delicacies widely appreciated in Europe. Two commercially exploited populations of sea urchins were characterized, for the first time, in terms of gonad quality (market-related traits such as colour, texture and nutritional value) and safety (contaminants levels) to define the best season for harvesting this valuable resource. Spawning occurred between spring-summer, yet gonad index peaked only in one population during winter. In both populations, the strongest yellow colouration and highest gonad firmness, protein, lipid, polyunsaturated fatty acids and carotenoid contents as well as the lowest contents of mineral were registered in fall. Inorganic and organic contaminants levels were, in general, similar among sites and seasons, being found at trace levels in seawater and gonads, with low risk for consumers. For these populations, high-quality gonads can be harvested during fall, despite being safe for human consumption all year round., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
182. Biodegradation of enrofloxacin by microbial consortia obtained from rhizosediments of two estuarine plants.
- Author
-
Santos F, Mucha AP, Alexandrino DAM, Almeida CMR, and Carvalho MF
- Subjects
- Biodegradation, Environmental, Enrofloxacin, Phylogeny, Wetlands, Microbial Consortia, Water Pollutants, Chemical
- Abstract
This study aimed to investigate the potential of microbial communities from the rhizosediment of two plants - Phragmites australis and Juncus maritimus - occurring in an estuarine area subjected to a high anthropogenic impact, to biodegrade ENR, a commonly used veterinary antibiotic. An enrichment process with 1 mgL
-1 of ENR was conducted during ca. 9 months, using acetate as a co-substrate. After this, the enriched microbial consortia were challenged with higher ENR concentrations of 2 and 3 mgL-1 . Microbial cultures enriched with 1 mgL-1 of ENR were capable of biodegrading this antibiotic, though not completely. By the end of the enrichment phase, microbial cultures were defluorinating an average of 50% of the ENR supplemented. Higher ENR concentrations led to lower biodegradation performances, suggesting a possible toxic/inhibitory effect in the microbial cultures. Phylogenetic identification of the microorganisms isolated from microbial cultures enriched with ENR revealed a high taxonomical diversity, with microorganisms belonging mainly to Proteobacteria and Bacteroidetes phyla. Assemblage of the obtained isolated strains (according to the enriched cultures from which they were isolated) revealed that the resulting consortia were also capable of degrading ENR, indicating that the main microbial players in the biodegradation of this antibiotic were isolated. These consortia also showed to be more robust to degrade higher concentrations of ENR than the corresponding enriched cultures. This study shows that microorganisms derived from rhizosediments of the selected plants, exhibit capacity to biodegrade ENR, though not completely for the concentrations tested, and may be further explored for the development of bioremediation strategies for the treatment of this antibiotic., (Copyright © 2018 Elsevier Ltd. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
183. Bacterial community dynamic associated with autochthonous bioaugmentation for enhanced Cu phytoremediation of salt-marsh sediments.
- Author
-
Almeida CMR, Oliveira T, Reis I, Gomes CR, and Mucha AP
- Subjects
- Bacteria classification, Copper metabolism, Estuaries, Geologic Sediments chemistry, Geologic Sediments microbiology, Microbial Consortia, Poaceae, Water Pollutants, Chemical metabolism, Wetlands, Bacteria growth & development, Biodegradation, Environmental, Copper analysis, Water Microbiology, Water Pollutants, Chemical analysis
- Abstract
Autochthonous bioaugmentation for metal phytoremediation is still little explored, particularly its application to estuarine salt marshes, but results obtained so far are promising. Nevertheless, understanding the behaviour of the microbial communities in the process of bioaugmentation and their role in improving metal phytoremediation is very important to fully validate the application of this biological technology. This study aimed to characterize the bacterial community dynamic associated with the application of autochthonous bioaugmentation in an experimentation which showed that Phragmites australis rhizosphere microorganisms could increase this salt marsh plant potential to phytoremediate Cu contaminated sediments. Bacterial communities present in the autochthonous microbial consortium resistant to Cu added to the medium and in the sediment at the beginning and at the end of the experiment were characterized by ARISA. Complementarily, the consortium and the sediment used for its production were characterized by next generation sequencing using the pyrosequencing platform 454. The microbial consortium resistant to Cu obtained from non-vegetated sediment was dominated by the genus Lactococcus (46%), Raoultella (25%), Bacillus (12%) and Acinetobacter (11%), whereas the one obtained form rhizosediment was dominated by the genus Gluconacetobacter (77%), Bacillus (17%) and Dyella (3%). Results clearly showed that, after two months of experiment, Cu caused a shift in the bacterial community structure of sediments, an effect that was observed either with or without addition of the metal resistant microbial consortium. Therefore, bioaugmentation application improved the process of phytoremediation (metal translocation by the plant was increased) without inducing long term changes in the bacterial community structure of the sediments. So, phytoremediation combined with autochthonous bioaugmentation can be a suitable technology for the recovery of estuarine areas, contributing for an efficient risk management strategy of these coastal zones., (Copyright © 2017. Published by Elsevier Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
184. Impacts of Silver Nanoparticles on a Natural Estuarine Plankton Community.
- Author
-
Baptista MS, Miller RJ, Halewood ER, Hanna SK, Almeida CM, Vasconcelos VM, Keller AA, and Lenihan HS
- Subjects
- Animals, Fluorescence, Heterotrophic Processes drug effects, Photosynthesis drug effects, Phototrophic Processes drug effects, Phytoplankton drug effects, Pigments, Biological metabolism, Zooplankton drug effects, Ecosystem, Estuaries, Metal Nanoparticles toxicity, Plankton drug effects, Silver toxicity
- Abstract
Potential effects of metal nanoparticles on aquatic organisms and food webs are hard to predict from the results of single-species tests under controlled laboratory conditions, and more realistic exposure experiments are rarely conducted. We tested whether silver nanoparticles (Ag NPs) had an impact on zooplankton grazing on their prey, specifically phytoplankton and bacterioplankton populations. If Ag NPs directly reduced the abundance of prey, thereby causing the overall rate of grazing by their predators to decrease, a cascading effect on a planktonic estuarine food web would be seen. Our results show that the growth rates of both phytoplankton and bacterioplankton populations were significantly reduced by Ag NPs at concentrations of ≥500 μg L(-1). At the same time, grazing rates on these populations tended to decline with exposure to Ag NPs. Therefore, Ag NPs did not cause a cascade of effects through the food web but impacted a specific trophic level. Photosynthetic efficiency of the phytoplankton was significantly reduced at Ag NPs concentrations of ≥500 μg L(-1). These effects did not occur at relatively low concentrations of Ag that are often toxic to single species of bacteria and other organisms, suggesting that the impacts of Ag NP exposure may not be apparent at environmentally relevant concentrations due to compensatory processes at the community level.
- Published
- 2015
- Full Text
- View/download PDF
185. Microbial community dynamics associated with veterinary antibiotics removal in constructed wetlands microcosms.
- Author
-
Fernandes JP, Almeida CMR, Pereira AC, Ribeiro IL, Reis I, Carvalho P, Basto MCP, and Mucha AP
- Subjects
- Anti-Bacterial Agents isolation & purification, Anti-Bacterial Agents pharmacology, Biodegradation, Environmental, Biodiversity, Enrofloxacin, Fluoroquinolones metabolism, Poaceae, Tetracycline metabolism, Tetracycline pharmacology, Veterinary Drugs isolation & purification, Veterinary Drugs pharmacology, Wastewater chemistry, Wastewater toxicity, Anti-Bacterial Agents metabolism, Microbial Consortia drug effects, Veterinary Drugs metabolism, Water Pollutants, Chemical metabolism, Water Purification methods, Wetlands
- Abstract
This study aimed to evaluate the response of the microbial community from CWs microcosms tested for the removal of two veterinary antibiotics, enrofloxacin (ENR) and tetracycline (TET), from livestock industry wastewater. Three treatments were tested (control, ENR or TET (100 μg L(-1))) over 12 weeks in microcosms unplanted and planted with Phragmites australis. CWs removal efficiency was relatively stable along time, with removals higher than 98% for ENR and 94% for TET. In addition, CWs were able to reduce wastewater toxicity, independently of antibiotics presence. Despite no significant differences were observed in terms of microbial abundance, bacterial richness or diversity, analysis of similarities (two-way crossed ANOSIM) showed a significant effect of both time and treatments in bacterial community structure. This study points to CWs applicability for veterinary antibiotics removal from livestock wastewaters, showing that CWs microbial communities were able to adapt without significant changes in their diversity or depuration capacity., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
186. Salt marsh sediment characteristics as key regulators on the efficiency of hydrocarbons bioremediation by Juncus maritimus rhizospheric bacterial community.
- Author
-
Ribeiro H, Almeida CM, Magalhães C, Bordalo AA, and Mucha AP
- Subjects
- Biodegradation, Environmental, Estuaries, Hydrocarbons analysis, Magnoliopsida growth & development, Magnoliopsida metabolism, Microbiota genetics, Petroleum Pollution, Phylogeny, Plant Roots metabolism, Plant Roots microbiology, Plants metabolism, Plants microbiology, Rhizosphere, Sodium Chloride metabolism, Soil Microbiology, Soil Pollutants analysis, Wetlands, Geologic Sediments chemistry, Hydrocarbons metabolism, Magnoliopsida microbiology, Soil Pollutants metabolism
- Abstract
Mitigation of petroleum hydrocarbons was investigated during a 5-month greenhouse experiment, to assess the rhizoremediation (RR) potential in sediments with different characteristics colonized by Juncus maritimus, a salt marsh plant commonly found in temperate estuaries. Furthermore, the efficiency of two bioremediation treatments namely biostimulation (BS) by the addition of nutrients, and bioaugmentation (BA) by addition of indigenous microorganisms, was tested in combination with RR. The effect of the distinct treatments on hydrocarbon degradation, root biomass weight, and bacterial community structure was assessed. Our result showed higher potential for hydrocarbon degradation (evaluated by total petroleum hydrocarbon analysis) in coarse rhizosediments with low organic matter (OM), than rhizosediments with high OM, and small size particles. Moreover, the bacterial community structure was shaped according to the rhizosediment characteristics, highlighting the importance of specific microbe-particle associations to define the structure of rhizospheric bacterial communities, rather than external factors, such as hydrocarbon contamination or the applied treatments. The potential for hydrocarbon RR seems to depend on root system development and bacterial diversity, since biodegradation efficiencies were positively related with these two parameters. Treatments with higher root biomass, and concomitantly with higher bacterial diversity yielded higher hydrocarbon degradation. Moreover, BS and BA did not enhance hydrocarbons RR. In fact, it was observed that higher nutrient availability might interfere with root growth and negatively influence hydrocarbon degradation performance. Therefore, our results suggested that to conduct appropriate hydrocarbon bioremediation strategies, the effect of sediment characteristics on root growth/exploration should be taken into consideration, a feature not explored in previous studies. Furthermore, strategies aiming for the recovery of bacterial diversity after oil spills may improve the efficiency of hydrocarbon biodegradation in contaminated salt marsh sediments.
- Published
- 2015
- Full Text
- View/download PDF
187. Potential of phytoremediation for the removal of petroleum hydrocarbons in contaminated salt marsh sediments.
- Author
-
Ribeiro H, Mucha AP, Almeida CM, and Bordalo AA
- Subjects
- Biodegradation, Environmental, Estuaries, Geologic Sediments microbiology, Magnoliopsida microbiology, Petroleum, Petroleum Pollution, Plant Roots metabolism, Plant Roots microbiology, Wetlands, Hydrocarbons metabolism, Magnoliopsida metabolism, Water Pollutants, Chemical metabolism
- Abstract
Degradation of petroleum hydrocarbons in colonized and un-colonized sediments by salt marsh plants Juncus maritimus and Phragmites australis collected in a temperate estuary was investigated during a 5-month greenhouse experiment. The efficiency of two bioremediation treatments namely biostimulation (BS) by the addition of nutrients, and bioaugmentation (BA) by addition of indigenous microorganisms was tested in comparison with hydrocarbon natural attenuation in un-colonized and with rhizoremediation in colonized sediments. Hydrocarbon degrading microorganisms and root biomass were assessed as well as hydrocarbon degradation levels. During the study, hydrocarbon degradation in un-colonized sediments was negligible regardless of treatments. Rhizoremediation proved to be an effective strategy for hydrocarbon removal, yielding high rates in most experiments. However, BS treatments showed a negative effect on the J. maritimus potential for hydrocarbon degradation by decreasing the root system development that lead to lower degradation rates. Although both plants and their associated microorganisms presented a potential for rhizoremediation of petroleum hydrocarbons in contaminated salt marsh sediments, results highlighted that nutrient requirements may be distinct among plant species, which should be accounted for when designing cleanup strategies., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
188. A strategy to potentiate Cd phytoremediation by saltmarsh plants - autochthonous bioaugmentation.
- Author
-
Nunes da Silva M, Mucha AP, Rocha AC, Teixeira C, Gomes CR, and Almeida CM
- Subjects
- Biodegradation, Environmental, Estuaries, Microbial Consortia, Rhizosphere, Cadmium metabolism, Magnoliopsida metabolism, Water Pollutants, Chemical metabolism
- Abstract
The recovery of estuarine environments is in need. Phytoremediation could be a valid option to reduce pollution while preserving natural biodiversity. In this work, estuarine sediments colonized by Juncus maritimus or Phragmites australis were spiked with cadmium in the absence and in the presence of an autochthonous microbial consortium resistant to the metal. The aim of this study was to increase the potential for cadmium phytoremediation that these two halophyte plants have shown. Experiments were carried out in greenhouses with an automatic irrigation system that simulated estuarine tidal cycles. After 2 months, Cd concentration in P. australis stems increased up to 7 times when the rhizosphere was inoculated with the microbial consortium. So, P. australis phytoextraction potential was increased through autochthonous bioaugmentation. As for J. maritimus, up to 48% more Cd (total amount) was observed in its belowground tissues after being subjected to autochthonous bioaugmentation. Therefore, the phytostabilization potential of this plant was promoted. For both plants this increase in cadmium uptake did not cause significant signs of toxicity. Therefore, the addition of autochthonous microorganisms resistant to cadmium seems to be a valuable strategy to potentiate phytoremediation of this metal in saltmarshes, being useful for the recovery of moderately impacted estuaries. This will contribute for an effective management of these areas. Research on this topic regarding estuarine ecosystems, especially saltmarshes, is, to our knowledge, inexistent., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
189. Bacterial community response to petroleum contamination and nutrient addition in sediments from a temperate salt marsh.
- Author
-
Ribeiro H, Mucha AP, Almeida CM, and Bordalo AA
- Subjects
- Analysis of Variance, Biodegradation, Environmental, Cell Count, DNA Primers genetics, Petroleum metabolism, Plant Roots metabolism, Portugal, RNA, Ribosomal genetics, Bacteria drug effects, Biota drug effects, Geologic Sediments analysis, Petroleum toxicity, Plant Roots microbiology, Poaceae metabolism, Wetlands
- Abstract
Microbial communities play an important role in the biodegradation of organic pollutants in sediments, including hydrocarbons. The aim of this study was to evaluate the response of temperate salt marsh microbial communities to petroleum contamination, in terms of community structure, abundance and capacity to degrade hydrocarbons. Sediments un-colonized and colonized (rhizosediments) by Juncus maritimus, Phragmites australis and Triglochin striata were collected in a temperate estuary (Lima, NW Portugal), spiked with petroleum under variable nutritional conditions, and incubated for 15 days. Results showed that plant speciation emerged as the major factor for shaping the rhizosphere community structure, overriding the petroleum influence. Moreover, when exposed to petroleum contamination, the distinct salt marsh microbial communities responded similarly with (i) increased abundance, (ii) changes in structure, and (iii) decreased diversity. Communities, particularly those associated to J. maritimus and P. australis roots displayed a potential to degrade petroleum hydrocarbons, with degradation percentages between 15% and 41%, depending on sediment type and nutritional conditions. In conclusion, distinct salt marsh microbial communities responded similarly to petroleum contamination, but presented different pace, nutritional requirements, and potential for its biodegradation, which should be taken into account when developing bioremediation strategies., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
190. Funding decisions: Romania needs overseas reviewers.
- Author
-
Altosaar I, Oehmen A, Almeida CM, De Pascale S, de Tommasi N, Domínguez de María P, Orzaez D, Zervos A, and Xu C
- Subjects
- Peer Review, Research trends, Research Support as Topic economics, Romania, Peer Review, Research standards, Research Support as Topic trends
- Published
- 2012
- Full Text
- View/download PDF
191. ICP-MS multi-element analysis of wine samples - a comparative study of the methodologies used in two laboratories.
- Author
-
Almeida CM, Vasconcelos MT, Barbaste M, and Medina B
- Subjects
- Mass Spectrometry methods, Metals analysis, Wine analysis
- Abstract
A comparison of the performance of the methodologies used in two distinct laboratories (Lab A and Lab B) for multi-element analysis in different wines was carried out. ICP-MS apparatus (quadrupole mass analyzers) of different brands as well as different wine pre-treatments were used. At Lab A, a pre-treatment by UV-irradiation was performed. At Lab B, a micro-concentric nebulizer was used for direct analysis of the wine. Twenty-six elements (Li, V, Co, Ni, Cu, Zn, Ga, As, Rb, Sr, Ba, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, and Pb) were measured in common at the two labs in three different wine samples (red and white Bordeaux table wines and Port wine) and the results were compared. The two methodologies provided similar LODs and similar precisions, with RSDs of 0.5-5%, for most of the elements. The recovery percentages were 85-120% at Lab A for the three wines, and 78-119% at Lab B for the Bordeaux wines, validating the accuracy of the methods used. Comparable results were obtained at both labs for ten elements (Li, V, Co, Ni, Cu, Zn, Rb, Sr, Ba, and Pb) in the three selected wines; the differences were lower than 10% in most cases. For REEs, the differences observed were slightly higher, but still in the acceptable range due to the sub-ppb levels involved. The results obtained for As and Ga were not comparable, due to methodological influence. A comparison through linear least-squares adjustment indicated that the results obtained by the two labs were linearly correlated (correlation coefficient =0.997) but statistically different as the slope was slightly, but significantly different from one, for a confidence level of 95% (the intercept was statistically identical to zero in any case). In the future, strictly more identical results can be achieved by using a reference wine sample.
- Published
- 2002
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.